Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003643

Physical Location & Seq

Genomic Coordinates Forward (+)

24542301 .. 24548030

Gene Structure 5730 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1191800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGTGGTCATTGGCACGAAACACCAGAAAAATGACACGTCTCCTCTTCCTAAACCATTCACTCTCTTCCAATCCCATTCCCAAACCCACCATTTCCAACGCTTACACCTCTTTCCACATCCCTCCCTCCAGTCTTTCTACCTCTGCATTTCTTCAAACAGTTCGGAGCCCTGCAATTCGTGCGTTTTGTTCGAATCCCAGCGGTGCAGCGAGGGTTGGGTCAGTACACGCAGCACCCAGCATGCAGAATCCGGGGGATATATCGTACCTGACGCAGCGTGAGGCTGCTGAGATCGATGAGGTTCTCATGGGTCCTCTCGGCTTCAGCGTCGACCAGTTAATGGAATTGGCTGGTTTGAGCGTCGCCACTTCCATTGCAGAGGTCTATAAACCGAGTGAGTATAACCGTGTCCTCACAATTTGTGGCCCTGGAAACAACGGTGGTGATGGTCTTGTGGCTGCTCGTCACCTGCATCACTTTGGATATAAGCCGTCTGTTTGTAATCCAAAGCGTACTGCTAAGCCTCTTTATGCTGGTCTTGTTACGCAACTGGAATCACTCTCAGTCCCTTTCATCTCAGTGGAAGATCTGCCGTTGGACTTGTCAAAGGACTTTGACATTATTGTAGATGCAATGTTTGGTTTCTCATTCCATGGTTCCCCAAGGCCTCCCTTTGATAACCTGATCCAAAAGCTTGTTTGTGTAAATAAATATGACCAAACTCGCGAAAAGTTGTCTGTTATTGTTTCCATAGATATTCCATCTGGGTGGCATGTCGAAGGGGATGGTGGTATTGAAGGCATTAAGCCTGACATGTTGGTTTCTCTGACTGCTCCAAAGTTGTGTGCAAAGAAATTTTTTGGTCCACACCATTTTCTGGGTGGTAGATTTGTCCCACCATCTATTGCCGATAAATATAAGCTTCGCCTTCCACCATATCCAGGCACTTCTATGTGTGTCCGTATTGGAAGGCCTCCACAAGTTGATATATCAGCTCTTAGAGAGAACTATATTTCTCCTGAGTTTCTTGAGGAACAGGTGGAGGCTGACCCCTTTGATCAGTTCCGCAAATGGTTCAACGATGTAGTGGCTGCTGGTTTGAGGGAACCAAATGCTATGGCATTGTCAACGGCTGCCAAGAACGGAAAAATCTCATCACGAATGGTATTGCTCAAAGGATTTGATAAGAACGGTTTTGTCTGGTACACAAACTATGAAAGTCGAAAGGCACATGAATTGTCTGGAAATCCTCATGCATCTCTTCTTTTCTACTGGGATAGTCTTAATCGGCAGGTAAGAGTAGAAGGGTCTGTGCAGAAAGTTTCTGATGAGGAATCTGATCAATACTTCCATAGCCGTCCTCGAGGCAGTCAGATTGGAGCAATAGTTAGCAAGCAGAGCACTGTTGTTCCCGGAAGGCATGTTCTGTATGACCAATACAAAGAACTAGAAGAAAAGTTCTCTGATGGAAGTTTAATTCCAAAACCTAAAAACTGGGGAGGATACAGACTTAAGCCAGAGATGTTTGAATTTTGGCAAGGCCAAAAATCTCGTTTGCATGACAGGCTACGATATTCTCCTGAAGAGATCAATGGACAAAAAGTGTGGAAAATTGAGCGGTTGGCGCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004733 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006081 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006739 GO:0006753 GO:0006766 GO:0006767 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009117 GO:0009165 GO:0009435 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016638 GO:0016641 GO:0016853 GO:0016854 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019637 GO:0019674 GO:0031974 GO:0031981 GO:0034356 GO:0034641 GO:0034654 GO:0042364 GO:0042816 GO:0042819 GO:0042822 GO:0042823 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044421 GO:0044422 GO:0044424 GO:0044428 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046184 GO:0046483 GO:0046496 GO:0051186 GO:0051188 GO:0052856 GO:0052857 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0072524 GO:0072525 GO:0090407 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00750 ko01100 ko01120 map00750 map01100 map01120

Pfam Domains

Protein Families

PNP_phzG_C Putative_PNPOx YjeF_N

Protein Analysis

544

Amino Acids

60.61

Weight (kDa)

8.87

Isoelectric Point (pI)

33.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YjeF_N	PF03853	111 - 282	5.7e-34	YjeF-related protein N-terminus
PNPOx_N	PF01243	361 - 484	4.7e-28	Pyridoxamine 5'-phosphate oxidase
PNP_phzG_C	PF10590	499 - 543	2.9e-20	Pyridoxine 5'-phosphate oxidase C-terminal dimerisation region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49970 AT5G49970
fragaria_vesca	FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940 FvH4_5g35940
malus_domestica	MD08G1191800.v1.1 MD15G1380600.v1.1
prunus_persica	Prupe.1G526800_v2.0.a1 Prupe.1G526800_v2.0.a1 Prupe.1G526800_v2.0.a1 Prupe.1G526800_v2.0.a1
pyrus_communis	pycom08g16530 pycom15g34090
rosa_chinensis	RchiOBHm_Chr7g0237681
rosa_laevigata	RLG00000000977
rosa_multiflora	Rmu_co8471315.1_g000001 Rmu_sc0007826.1_g000003
rosa_roxburghii	Rroxscaffold_3G00223890
rosa_rugosa	Rorug07G0306200 Rorug07G0306300 Rorug07G0306400 Rorug07G0306500
rosa_samantha	Rh7AG462900 Rh7BG433000 Rh7CG479900 Rh7DG448900
rosa_wichuraiana	Rw7G038290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	477
AbsI	CCTCGAGG	1 cut(s)	1362
Acc36I	ACCTGC	2 cut(s)	477, 1282
AccB1I	GGYRCC	1 cut(s)	1624
AccB7I	CCANNNNNTGG	2 cut(s)	340, 877
AccBSI	CCGCTC	1 cut(s)	1618
AccI	GTMKAC	1 cut(s)	330
AccII	CGCG	1 cut(s)	726
AciI	CCGC	3 cut(s)	201, 1066, 1618
AclWI	GGATC	1 cut(s)	679
AcsI	RAATTY	2 cut(s)	854, 1529
AcuI	CTGAAG	2 cut(s)	307, 1602
AcyI	GRCGYC	1 cut(s)	1625
AfaI	GTAC	4 cut(s)	225, 266, 514, 1205
AfiI	CCNNNNNNNGG	3 cut(s)	340, 877, 941
AflII	CTTAAG	1 cut(s)	1511
AflIII	ACRYGT	2 cut(s)	35, 813
AgsI	TTSAA	4 cut(s)	155, 797, 1078, 1529
AjiI	CACGTC	1 cut(s)	38
AjnI	CCWGG	2 cut(s)	427, 940
AloI	GAACNNNNNNTCC	2 cut(s)	1407, 1439
AluBI	AGCT	3 cut(s)	694, 922, 995
AluI	AGCT	3 cut(s)	694, 922, 995
Alw21I	GWGCWC	1 cut(s)	1403
Alw26I	GTCTC	1 cut(s)	44
AlwI	GGATC	1 cut(s)	679
Ama87I	CYCGRG	1 cut(s)	1362
AoxI	GGCC	4 cut(s)	424, 665, 971, 1540
ApeKI	GCWGC	7 cut(s)	206, 230, 274, 284, 458, 1091, 1133
ApoI	RAATTY	2 cut(s)	854, 1529
AspLEI	GCGC	1 cut(s)	1627
AspS9I	GGNCC	3 cut(s)	311, 425, 863
AsuC2I	CCSGG	2 cut(s)	252, 1413
AsuHPI	GGTGA	2 cut(s)	455, 458
AsuII	TTCGAA	1 cut(s)	191
AvaI	CYCGRG	1 cut(s)	1362
AvaII	GGWCC	2 cut(s)	311, 863
BanI	GGYRCC	1 cut(s)	1624
BanII	GRGCYC	1 cut(s)	170
Bbv12I	GWGCWC	1 cut(s)	1403
BbvI	GCAGC	7 cut(s)	218, 242, 271, 286, 445, 1078, 1120
BccI	CCATC	5 cut(s)	440, 769, 779, 907, 1460
BceAI	ACGGC	4 cut(s)	475, 577, 1146, 1341
BciT130I	CCWGG	2 cut(s)	429, 942
BciVI	GTATCC	1 cut(s)	1496
BclI	TGATCA	2 cut(s)	1057, 1339
BcnI	CCSGG	2 cut(s)	252, 1413
BcoDI	GTCTC	1 cut(s)	44
BfaI	CTAG	1 cut(s)	1448
BfoI	RGCGCY	1 cut(s)	1628
BfrI	CTTAAG	1 cut(s)	1511
BfuAI	ACCTGC	2 cut(s)	477, 1282
BfuI	GTATCC	1 cut(s)	1496
BglII	AGATCT	1 cut(s)	586
BisI	GCNGC	7 cut(s)	207, 231, 275, 285, 459, 1092, 1134
BlpI	GCTNAGC	1 cut(s)	519
BlsI	GCNGC	7 cut(s)	208, 232, 276, 286, 460, 1093, 1135
Bme1390I	CCNGG	4 cut(s)	252, 429, 942, 1413
Bme18I	GGWCC	2 cut(s)	311, 863
BmeT110I	CYCGRG	1 cut(s)	1362
BmgBI	CACGTC	1 cut(s)	38
BmgT120I	GGNCC	3 cut(s)	311, 425, 863
BmiI	GGNNCC	5 cut(s)	167, 312, 658, 1107, 1626
BmrFI	CCNGG	4 cut(s)	252, 429, 942, 1413
BmrI	ACTGGG	2 cut(s)	1282, 1504
BmsI	GCATC	3 cut(s)	481, 619, 1265
BmuI	ACTGGG	2 cut(s)	1282, 1504
BpmI	CTGGAG	1 cut(s)	112
Bpu1102I	GCTNAGC	1 cut(s)	519
Bpu14I	TTCGAA	1 cut(s)	191
BpuEI	CTTGAG	1 cut(s)	1049
BpuMI	CCSGG	2 cut(s)	252, 1413
Bsa29I	ATCGAT	1 cut(s)	294
BsaHI	GRCGYC	1 cut(s)	1625
BsaJI	CCNNGG	4 cut(s)	251, 427, 652, 662
BsaXI	ACNNNNNCTCC	2 cut(s)	1371, 1401
Bsc4I	CCNNNNNNNGG	3 cut(s)	340, 877, 941
Bse1I	ACTGG	5 cut(s)	129, 334, 555, 1277, 1499
Bse3DI	GCAATG	2 cut(s)	372, 639
BseBI	CCWGG	2 cut(s)	429, 942
BseCI	ATCGAT	1 cut(s)	294
BseDI	CCNNGG	4 cut(s)	251, 427, 652, 662
BseGI	GGATG	2 cut(s)	117, 790
BseLI	CCNNNNNNNGG	3 cut(s)	340, 877, 941
BseMI	GCAATG	2 cut(s)	372, 639
BseMII	CTCAG	4 cut(s)	279, 576, 591, 1011
BseNI	ACTGG	5 cut(s)	129, 334, 555, 1277, 1499
BseRI	GAGGAG	1 cut(s)	32
BseXI	GCAGC	7 cut(s)	218, 242, 271, 286, 445, 1078, 1120
BseYI	CCCAGC	2 cut(s)	197, 236
BsgI	GTGCAG	2 cut(s)	225, 1334
Bsh1236I	CGCG	1 cut(s)	726
BshFI	GGCC	4 cut(s)	426, 667, 973, 1542
BshNI	GGYRCC	1 cut(s)	1624
BshVI	ATCGAT	1 cut(s)	294
BsiHKAI	GWGCWC	1 cut(s)	1403
BsiHKCI	CYCGRG	1 cut(s)	1362
BsiSI	CCGG	2 cut(s)	251, 1413
BslFI	GGGAC	2 cut(s)	551, 878
BslI	CCNNNNNNNGG	3 cut(s)	340, 877, 941
BsmAI	GTCTC	1 cut(s)	44
BsmBI	CGTCTC	1 cut(s)	44
BsmFI	GGGAC	2 cut(s)	551, 878
BsnI	GGCC	4 cut(s)	426, 667, 973, 1542
BsoBI	CYCGRG	1 cut(s)	1362
Bsp119I	TTCGAA	1 cut(s)	191
Bsp1286I	GDGCHC	2 cut(s)	170, 1403
Bsp143I	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
Bsp1720I	GCTNAGC	1 cut(s)	519
Bsp19I	CCATGG	1 cut(s)	652
Bsp68I	TCGCGA	1 cut(s)	726
BspACI	CCGC	3 cut(s)	201, 1066, 1618
BspANI	GGCC	4 cut(s)	426, 667, 973, 1542
BspCNI	CTCAG	4 cut(s)	280, 575, 590, 1012
BspDI	ATCGAT	1 cut(s)	294
BspFNI	CGCG	1 cut(s)	726
BspLI	GGNNCC	5 cut(s)	167, 312, 658, 1107, 1626
BspMI	ACCTGC	2 cut(s)	477, 1282
BspPI	GGATC	1 cut(s)	679
BspT104I	TTCGAA	1 cut(s)	191
BspT107I	GGYRCC	1 cut(s)	1624
BspTI	CTTAAG	1 cut(s)	1511
BsrBI	CCGCTC	1 cut(s)	1618
BsrDI	GCAATG	2 cut(s)	372, 639
BsrI	ACTGG	5 cut(s)	129, 334, 555, 1277, 1499
BssECI	CCNNGG	4 cut(s)	251, 427, 652, 662
BssMI	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
BssNI	GRCGYC	1 cut(s)	1625
BssT1I	CCWWGG	2 cut(s)	652, 662
Bst2UI	CCWGG	2 cut(s)	429, 942
Bst4CI	ACNGT	5 cut(s)	160, 407, 440, 1193, 1405
Bst6I	CTCTTC	4 cut(s)	50, 70, 1266, 1578
BstACI	GRCGYC	1 cut(s)	1625
BstAFI	CTTAAG	1 cut(s)	1511
BstAPI	GCANNNNNTGC	1 cut(s)	179
BstBI	TTCGAA	1 cut(s)	191
BstC8I	GCNNGC	2 cut(s)	242, 1394
BstDEI	CTNAG	6 cut(s)	288, 519, 562, 577, 998, 1020
BstDSI	CCRYGG	1 cut(s)	652
BstF5I	GGATG	2 cut(s)	117, 790
BstFNI	CGCG	1 cut(s)	726
BstH2I	RGCGCY	1 cut(s)	1628
BstHHI	GCGC	1 cut(s)	1627
BstKTI	GATC	6 cut(s)	294, 589, 687, 1060, 1342, 1590
BstMAI	GTCTC	1 cut(s)	44
BstMBI	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
BstMWI	GCNNNNNNNGC	4 cut(s)	179, 1398, 1564, 1624
BstNI	CCWGG	2 cut(s)	429, 942
BstNSI	RCATGY	4 cut(s)	244, 776, 817, 1424
BstSCI	CCNGG	4 cut(s)	250, 427, 940, 1411
BstUI	CGCG	1 cut(s)	726
BstV1I	GCAGC	7 cut(s)	218, 242, 271, 286, 445, 1078, 1120
BstX2I	RGATCY	1 cut(s)	586
BstXI	CCANNNNNNTGG	1 cut(s)	768
BstYI	RGATCY	1 cut(s)	586
Bsu15I	ATCGAT	1 cut(s)	294
BsuI	GTATCC	1 cut(s)	1496
BsuRI	GGCC	4 cut(s)	426, 667, 973, 1542
BsuTUI	ATCGAT	1 cut(s)	294
BtgI	CCRYGG	1 cut(s)	652
BtrI	CACGTC	1 cut(s)	38
BtsCI	GGATG	2 cut(s)	117, 790
BtsIMutI	CAGTG	2 cut(s)	585, 1401
BtuMI	TCGCGA	1 cut(s)	726
BveI	ACCTGC	2 cut(s)	477, 1282
Cac8I	GCNNGC	2 cut(s)	242, 1394
CfoI	GCGC	1 cut(s)	1627
Cfr13I	GGNCC	3 cut(s)	311, 425, 863
ClaI	ATCGAT	1 cut(s)	294
CseI	GACGC	3 cut(s)	280, 316, 349
Csp6I	GTAC	4 cut(s)	224, 265, 513, 1204
CspCI	CAANNNNNGTGG	4 cut(s)	355, 390, 1586, 1621
CviAII	CATG	9 cut(s)	241, 307, 653, 773, 814, 1232, 1253, 1421, 1559
CviQI	GTAC	4 cut(s)	224, 265, 513, 1204
DdeI	CTNAG	6 cut(s)	288, 519, 562, 577, 998, 1020
DinI	GGCGCC	1 cut(s)	1626
DpnI	GATC	6 cut(s)	293, 588, 686, 1059, 1341, 1589
DpnII	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
Eam1104I	CTCTTC	4 cut(s)	50, 70, 1266, 1578
EarI	CTCTTC	4 cut(s)	50, 70, 1266, 1578
Eco130I	CCWWGG	2 cut(s)	652, 662
Eco147I	AGGCCT	2 cut(s)	667, 973
Eco24I	GRGCYC	1 cut(s)	170
Eco47I	GGWCC	2 cut(s)	311, 863
Eco57I	CTGAAG	2 cut(s)	307, 1602
Eco88I	CYCGRG	1 cut(s)	1362
EcoO109I	RGGNCCY	1 cut(s)	311
EcoRII	CCWGG	2 cut(s)	427, 940
EcoT14I	CCWWGG	2 cut(s)	652, 662
EcoT22I	ATGCAT	1 cut(s)	1258
EcoT38I	GRGCYC	1 cut(s)	170
EgeI	GGCGCC	1 cut(s)	1626
EheI	GGCGCC	1 cut(s)	1626
ErhI	CCWWGG	2 cut(s)	652, 662
Esp3I	CGTCTC	1 cut(s)	44
FaeI	CATG	9 cut(s)	244, 310, 656, 776, 817, 1235, 1256, 1424, 1562
FaqI	GGGAC	2 cut(s)	551, 878
FatI	CATG	9 cut(s)	240, 306, 652, 772, 813, 1231, 1252, 1420, 1558
FbaI	TGATCA	2 cut(s)	1057, 1339
FblI	GTMKAC	1 cut(s)	330
Fnu4HI	GCNGC	7 cut(s)	207, 231, 275, 285, 459, 1092, 1134
FokI	GGATG	2 cut(s)	104, 797
FriOI	GRGCYC	1 cut(s)	170
Fsp4HI	GCNGC	7 cut(s)	207, 231, 275, 285, 459, 1092, 1134
FspBI	CTAG	1 cut(s)	1448
GlaI	GCGC	1 cut(s)	1626
GluI	GCNGC	7 cut(s)	207, 231, 275, 285, 459, 1092, 1134
GsaI	CCCAGC	2 cut(s)	201, 240
GsuI	CTGGAG	1 cut(s)	112
HaeII	RGCGCY	1 cut(s)	1628
HaeIII	GGCC	4 cut(s)	426, 667, 973, 1542
HapII	CCGG	2 cut(s)	251, 1413
HgaI	GACGC	3 cut(s)	280, 316, 349
HhaI	GCGC	1 cut(s)	1627
Hin1I	GRCGYC	1 cut(s)	1625
Hin1II	CATG	9 cut(s)	244, 310, 656, 776, 817, 1235, 1256, 1424, 1562
Hin6I	GCGC	1 cut(s)	1625
HinP1I	GCGC	1 cut(s)	1625
HincII	GTYRAC	2 cut(s)	331, 1128
HindII	GTYRAC	2 cut(s)	331, 1128
HindIII	AAGCTT	2 cut(s)	692, 920
HinfI	GANTC	4 cut(s)	193, 247, 554, 1334
HpaII	CCGG	2 cut(s)	251, 1413
HphI	GGTGA	2 cut(s)	455, 458
Hpy166II	GTNNAC	5 cut(s)	226, 331, 866, 1128, 1206
Hpy188I	TCNGA	6 cut(s)	165, 828, 1327, 1339, 1374, 1465
Hpy188III	TCNNGA	6 cut(s)	725, 1019, 1028, 1158, 1242, 1580
Hpy8I	GTNNAC	5 cut(s)	226, 331, 866, 1128, 1206
Hpy99I	CGWCG	2 cut(s)	332, 365
HpyAV	CCTTC	6 cut(s)	773, 791, 938, 963, 1298, 1410
HpyCH4III	ACNGT	5 cut(s)	160, 407, 440, 1193, 1405
HpyCH4IV	ACGT	1 cut(s)	37
HpyF10VI	GCNNNNNNNGC	4 cut(s)	179, 1398, 1564, 1624
HpyF3I	CTNAG	6 cut(s)	288, 519, 562, 577, 998, 1020
HpySE526I	ACGT	1 cut(s)	37
Hsp92I	GRCGYC	1 cut(s)	1625
Hsp92II	CATG	9 cut(s)	244, 310, 656, 776, 817, 1235, 1256, 1424, 1562
HspAI	GCGC	1 cut(s)	1625
KasI	GGCGCC	1 cut(s)	1624
Ksp22I	TGATCA	2 cut(s)	1057, 1339
Kzo9I	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
LmnI	GCTCC	3 cut(s)	165, 838, 1379
Lsp1109I	GCAGC	7 cut(s)	218, 242, 271, 286, 445, 1078, 1120
LweI	GCATC	3 cut(s)	481, 619, 1265
MaeI	CTAG	1 cut(s)	1448
MaeII	ACGT	1 cut(s)	37
MaeIII	GTNAC	2 cut(s)	464, 541
MalI	GATC	6 cut(s)	293, 588, 686, 1059, 1341, 1589
MbiI	CCGCTC	1 cut(s)	1618
MboI	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
MboII	GAAGA	7 cut(s)	37, 57, 143, 596, 1253, 1463, 1595
MflI	RGATCY	1 cut(s)	586
MhlI	GDGCHC	2 cut(s)	170, 1403
MluCI	AATT	8 cut(s)	174, 344, 417, 854, 1235, 1476, 1529, 1611
Mly113I	GGCGCC	1 cut(s)	1625
MmeI	TCCRAC	2 cut(s)	120, 576
Mph1103I	ATGCAT	1 cut(s)	1258
MseI	TTAA	5 cut(s)	338, 804, 1284, 1475, 1512
MslI	CAYNNNNRTG	3 cut(s)	578, 766, 950
MspA1I	CMGCKG	1 cut(s)	201
MspCI	CTTAAG	1 cut(s)	1511
MspI	CCGG	2 cut(s)	251, 1413
MspR9I	CCNGG	4 cut(s)	252, 429, 942, 1413
MvaI	CCWGG	2 cut(s)	429, 942
MvnI	CGCG	1 cut(s)	726
MwoI	GCNNNNNNNGC	4 cut(s)	179, 1398, 1564, 1624
NarI	GGCGCC	1 cut(s)	1625
NciI	CCSGG	2 cut(s)	252, 1413
NcoI	CCATGG	1 cut(s)	652
NdeII	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
NlaIII	CATG	9 cut(s)	244, 310, 656, 776, 817, 1235, 1256, 1424, 1562
NlaIV	GGNNCC	5 cut(s)	167, 312, 658, 1107, 1626
NmeAIII	GCCGAG	1 cut(s)	297
NmuCI	GTSAC	1 cut(s)	464
NruI	TCGCGA	1 cut(s)	726
NsiI	ATGCAT	1 cut(s)	1258
NspI	RCATGY	4 cut(s)	244, 776, 817, 1424
NspV	TTCGAA	1 cut(s)	191
PaeI	GCATGC	1 cut(s)	244
PaeR7I	CTCGAG	1 cut(s)	1362
PaqCI	CACCTGC	1 cut(s)	477
PceI	AGGCCT	2 cut(s)	667, 973
PciI	ACATGT	1 cut(s)	813
PcsI	WCGNNNNNNNCGW	1 cut(s)	324
PfeI	GAWTC	4 cut(s)	193, 247, 554, 1334
PflMI	CCANNNNNTGG	2 cut(s)	340, 877
PfoI	TCCNGGA	1 cut(s)	1411
PkrI	GCNGC	7 cut(s)	208, 232, 276, 286, 460, 1093, 1135
PluTI	GGCGCC	1 cut(s)	1628
PpuMI	RGGWCCY	1 cut(s)	311
PscI	ACATGT	1 cut(s)	813
Psp5II	RGGWCCY	1 cut(s)	311
Psp6I	CCWGG	2 cut(s)	427, 940
PspFI	CCCAGC	2 cut(s)	197, 236
PspGI	CCWGG	2 cut(s)	427, 940
PspN4I	GGNNCC	5 cut(s)	167, 312, 658, 1107, 1626
PspPI	GGNCC	3 cut(s)	311, 425, 863
PspPPI	RGGWCCY	1 cut(s)	311
PspXI	VCTCGAGB	1 cut(s)	1362
PsuI	RGATCY	1 cut(s)	586
RruI	TCGCGA	1 cut(s)	726
RsaI	GTAC	4 cut(s)	225, 266, 514, 1205
RsaNI	GTAC	4 cut(s)	224, 265, 513, 1204
RseI	CAYNNNNRTG	3 cut(s)	578, 766, 950
SalI	GTCGAC	1 cut(s)	329
SaqAI	TTAA	5 cut(s)	338, 804, 1284, 1475, 1512
SatI	GCNGC	7 cut(s)	207, 231, 275, 285, 459, 1092, 1134
Sau3AI	GATC	6 cut(s)	291, 586, 684, 1057, 1339, 1587
Sau96I	GGNCC	3 cut(s)	311, 425, 863
ScrFI	CCNGG	4 cut(s)	252, 429, 942, 1413
SduI	GDGCHC	2 cut(s)	170, 1403
SfaNI	GCATC	3 cut(s)	481, 619, 1265
SfoI	GGCGCC	1 cut(s)	1626
Sfr274I	CTCGAG	1 cut(s)	1362
SfuI	TTCGAA	1 cut(s)	191
SinI	GGWCC	2 cut(s)	311, 863
SlaI	CTCGAG	1 cut(s)	1362
SmiMI	CAYNNNNRTG	3 cut(s)	578, 766, 950
SmlI	CTYRAG	3 cut(s)	1028, 1362, 1511
SmoI	CTYRAG	3 cut(s)	1028, 1362, 1511
SphI	GCATGC	1 cut(s)	244
Sse9I	AATT	8 cut(s)	174, 344, 417, 854, 1235, 1476, 1529, 1611
SseBI	AGGCCT	2 cut(s)	667, 973
SsiI	CCGC	3 cut(s)	201, 1066, 1618
SspDI	GGCGCC	1 cut(s)	1624
SspMI	CTAG	1 cut(s)	1448
StuI	AGGCCT	2 cut(s)	667, 973
StyD4I	CCNGG	4 cut(s)	250, 427, 940, 1411
StyI	CCWWGG	2 cut(s)	652, 662
TaaI	ACNGT	5 cut(s)	160, 407, 440, 1193, 1405
TaiI	ACGT	1 cut(s)	40
TaqI	TCGA	6 cut(s)	191, 294, 330, 777, 1222, 1363
TasI	AATT	8 cut(s)	174, 344, 417, 854, 1235, 1476, 1529, 1611
TatI	WGTACW	1 cut(s)	223
TfiI	GAWTC	4 cut(s)	193, 247, 554, 1334
Tru1I	TTAA	5 cut(s)	338, 804, 1284, 1475, 1512
Tru9I	TTAA	5 cut(s)	338, 804, 1284, 1475, 1512
TscAI	CASTG	2 cut(s)	585, 1408
TseFI	GTSAC	1 cut(s)	464
TseI	GCWGC	7 cut(s)	206, 230, 274, 284, 458, 1091, 1133
Tsp45I	GTSAC	1 cut(s)	464
TspDTI	ATGAA	3 cut(s)	562, 1230, 1248
TspGWI	ACGGA	2 cut(s)	950, 1158
TspRI	CASTG	2 cut(s)	585, 1408
Van91I	CCANNNNNTGG	2 cut(s)	340, 877
Vha464I	CTTAAG	1 cut(s)	1511
VpaK11BI	GGWCC	2 cut(s)	311, 863
XapI	RAATTY	2 cut(s)	854, 1529
XceI	RCATGY	4 cut(s)	244, 776, 817, 1424
XcmI	CCANNNNNNNNNTGG	1 cut(s)	878
XhoI	CTCGAG	1 cut(s)	1362
XmiI	GTMKAC	1 cut(s)	330
XspI	CTAG	1 cut(s)	1448
Zsp2I	ATGCAT	1 cut(s)	1258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.