Rorug07G0306500

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S- specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003643
Physical Location & Seq
Reverse (-)
30784532 .. 30790196
5665 bp
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UTR
Exon/CDS
Intron
Rorug07G0306500.1

Sequence Viewer

Length: 654 bp
ATGATCCTCTCCTGCTTCAAGTTAAATCCCTTCGAATATCTTAACTTACCATTTGATGCAACCGAAGAAGATGTTAAAAAACAGTATCGAAAGTTGTCCTTGATGGTTCACCCTGATAAATGCAAGCATCCACAGGCTAAGGAGGCATTTGGAGCATTGGCGAAAGCCCACCAACTTTTACTCGATCAACAAGAAAGGGACTATATCATTAGCCAAGTCACTGCTGCCAAAGACGAACTCAGAGCAAAAAGGAAGAAGCAGTTGAAGAAAGATACAGCTTCTAAAATAAAGTCATTGGTCGATGAGGGAAAATATGAGCAACAGTATGAGCAGTCTGAAGAGTTTCAGCAAGAACTAAAAATGAAGGTTCGAGAAATATTAACAGAACAAGAATGGCGGAGGAGAAAAATGCAAATGAGGATATCAGAAGAGGAAGGAAGATTGAAAAAAGATGAAGAAGAAGCCAAAGAGATGTGGAAAAGAAAACGAGATCATGAGGCAGAATGGGAAGGAACAAGAGAAAAAAGGGTTTCAAGCTGGAGAGATTTTATGAAGGGTGGAAAGAAGGCCAAGAAAGGAGAACTTCGACCTCCCAAACTGAAAACTGAAGATCCTAACAAGTCTTATGTTCAAAGGCCAGTAAAACGAGGCTGA

Protein Analysis

217

Amino Acids

26.0

Weight (kDa)

9.53

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 66 2.9e-16 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 397
AclWI GGATC 1 cut(s) 605
AcuI CTGAAG 2 cut(s) 357, 627
AgsI TTSAA 5 cut(s) 19, 265, 445, 534, 632
AluBI AGCT 2 cut(s) 278, 537
AluI AGCT 2 cut(s) 278, 537
AlwI GGATC 1 cut(s) 605
AoxI GGCC 2 cut(s) 567, 635
ApeKI GCWGC 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 101
AsuII TTCGAA 1 cut(s) 33
BbvI GCAGC 1 cut(s) 211
BccI CCATC 1 cut(s) 97
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmsI GCATC 2 cut(s) 46, 136
BpmI CTGGAG 1 cut(s) 559
Bpu10I CCTNAGC 1 cut(s) 138
Bpu14I TTCGAA 1 cut(s) 33
Bse1I ACTGG 1 cut(s) 638
BseGI GGATG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 253
BseNI ACTGG 1 cut(s) 638
BseRI GAGGAG 1 cut(s) 415
BseXI GCAGC 1 cut(s) 211
BshFI GGCC 2 cut(s) 569, 637
BslFI GGGAC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 2 cut(s) 569, 637
Bsp119I TTCGAA 1 cut(s) 33
Bsp143I GATC 4 cut(s) 3, 184, 490, 610
BspACI CCGC 1 cut(s) 397
BspANI GGCC 2 cut(s) 569, 637
BspCNI CTCAG 1 cut(s) 252
BspHI TCATGA 1 cut(s) 493
BspPI GGATC 1 cut(s) 605
BspT104I TTCGAA 1 cut(s) 33
BsrI ACTGG 1 cut(s) 638
BssMI GATC 4 cut(s) 3, 184, 490, 610
Bst4CI ACNGT 2 cut(s) 84, 324
Bst6I CTCTTC 2 cut(s) 333, 423
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 138, 239
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 4 cut(s) 6, 187, 493, 613
BstMBI GATC 4 cut(s) 3, 184, 490, 610
BstMWI GCNNNNNNNGC 2 cut(s) 143, 152
BstV1I GCAGC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 2 cut(s) 569, 637
BtsCI GGATG 1 cut(s) 127
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 125
CciI TCATGA 1 cut(s) 493
CviAII CATG 1 cut(s) 494
CviJI RGCY 9 cut(s) 137, 167, 213, 278, 464, 537, 569, 637, 651
CviKI_1 RGCY 9 cut(s) 137, 167, 213, 278, 464, 537, 569, 637, 651
DdeI CTNAG 2 cut(s) 138, 239
DpnI GATC 4 cut(s) 5, 186, 492, 612
DpnII GATC 4 cut(s) 3, 184, 490, 610
Eam1104I CTCTTC 2 cut(s) 333, 423
EarI CTCTTC 2 cut(s) 333, 423
EciI GGCGGA 1 cut(s) 412
Eco32I GATATC 1 cut(s) 423
Eco57I CTGAAG 2 cut(s) 357, 627
EcoRV GATATC 1 cut(s) 423
FaeI CATG 1 cut(s) 497
FaiI YATR 6 cut(s) 204, 315, 327, 495, 551, 627
FalI AAGNNNNNCTT 4 cut(s) 83, 115, 567, 599
FaqI GGGAC 1 cut(s) 212
FatI CATG 1 cut(s) 493
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 1 cut(s) 114
Fsp4HI GCNGC 1 cut(s) 225
GluI GCNGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 559
HaeIII GGCC 2 cut(s) 569, 637
Hin1II CATG 1 cut(s) 497
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 242, 337, 427
Hpy188III TCNNGA 2 cut(s) 371, 494
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 6 cut(s) 40, 358, 428, 503, 547, 559
HpyCH4III ACNGT 2 cut(s) 84, 324
HpyCH4V TGCA 3 cut(s) 59, 123, 412
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 152
HpyF3I CTNAG 2 cut(s) 138, 239
Hsp92II CATG 1 cut(s) 497
Kzo9I GATC 4 cut(s) 3, 184, 490, 610
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 4 cut(s) 25, 119, 126, 523
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 46, 136
MaeIII GTNAC 1 cut(s) 217
MalI GATC 4 cut(s) 5, 186, 492, 612
MboI GATC 4 cut(s) 3, 184, 490, 610
MflI RGATCY 1 cut(s) 610
MnlI CCTC 9 cut(s) 17, 136, 298, 393, 411, 424, 490, 600, 641
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 4 cut(s) 23, 42, 75, 380
MwoI GCNNNNNNNGC 2 cut(s) 143, 152
NdeII GATC 4 cut(s) 3, 184, 490, 610
NlaIII CATG 1 cut(s) 497
NmuCI GTSAC 1 cut(s) 217
NspV TTCGAA 1 cut(s) 33
PagI TCATGA 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 342
PkrI GCNGC 1 cut(s) 226
PsuI RGATCY 1 cut(s) 610
SaqAI TTAA 4 cut(s) 23, 42, 75, 380
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 4 cut(s) 3, 184, 490, 610
SetI ASST 4 cut(s) 280, 369, 539, 592
SfaNI GCATC 2 cut(s) 46, 136
SfuI TTCGAA 1 cut(s) 33
SsiI CCGC 1 cut(s) 397
SspI AATATT 1 cut(s) 378
TaaI ACNGT 2 cut(s) 84, 324
TaqI TCGA 6 cut(s) 33, 88, 183, 300, 370, 586
Tru1I TTAA 4 cut(s) 23, 42, 75, 380
Tru9I TTAA 4 cut(s) 23, 42, 75, 380
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 3 cut(s) 377, 468, 566
TspRI CASTG 1 cut(s) 226
XmnI GAANNNNTTC 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.