MD08G1192700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012630
Physical Location & Seq
Reverse (-)
24805098 .. 24806171
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1192700.v1.1.491

Sequence Viewer

Length: 672 bp
ATGACAACAAGCAGCAAAGGAAACACCACCACCACACCATCAGTGGTCCAAGGGGAAGCAGCAGGCAGCCGTGAGTGCTGCATGTGTGGGGATTTTGGCTTCTCCTATGAGCTCTTCCTTTGCAAGGTTTGCCAATTCAGATCTCAGCACAGGTACTGCAGCAATCTTTACCCAAATGCTGAGTCCTGCAGAATTTGCAATTGGTGCCTCACCCAGAAGGAGGACACAAAGGAGAAGTCCCAAAACTCCTCAAATTCTTCCTCCTCATGCAAAAAGGAAACCCAAGATCAAGACCATCTAATTACCAAGATCACCAAGAAAAAAAATAATCCATCAAAAAATAGTAATACTTCATTTGGTGGGTGTCTGCGGGGTACTATGCAGCTGCAACCGAGTGGGCCCATCAAGAAACAGAGATCACTTGATCTTCATCACCTTCATCGTCATTCGGCAGCAATATCTCCATCACCTTCACCATCTCCAAAATCTGCACCAGTTGTTACCAGAAAGAGGATCATCACAAAAGGGGAAAAGGAGGCTGAAAGGACCAGGAGGACACGGTCAGAGGACATATCAAACATAAACAACACTAGTACTATTGGGATCACAAGGCAAGTCTTCAGGAACAAGGTCAGAAGATACAAGCTTCTGGATGAAGTTTCAAGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

25.01

Weight (kDa)

9.68

Isoelectric Point (pI)

58.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD_pln PF25054 27 - 69 2.2e-20 PHD-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 204
AciI CCGC 1 cut(s) 370
AclWI GGATC 2 cut(s) 521, 611
AcsI RAATTY 2 cut(s) 192, 253
AcuI CTGAAG 1 cut(s) 604
AfaI GTAC 3 cut(s) 155, 376, 595
AfiI CCNNNNNNNGG 3 cut(s) 124, 220, 510
AgsI TTSAA 1 cut(s) 663
AhlI ACTAGT 1 cut(s) 590
AjnI CCWGG 1 cut(s) 548
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AluBI AGCT 3 cut(s) 112, 385, 646
AluI AGCT 3 cut(s) 112, 385, 646
Alw21I GWGCWC 1 cut(s) 114
AlwI GGATC 2 cut(s) 521, 611
AlwNI CAGNNNCTG 1 cut(s) 156
AoxI GGCC 1 cut(s) 398
ApaI GGGCCC 1 cut(s) 402
ApeKI GCWGC 8 cut(s) 12, 59, 66, 78, 159, 382, 385, 452
ApoI RAATTY 2 cut(s) 192, 253
ArsI GACNNNNNNTTYG 4 cut(s) 167, 199, 221, 253
AspS9I GGNCC 4 cut(s) 46, 398, 399, 546
AsuHPI GGTGA 5 cut(s) 202, 304, 425, 459, 465
AvaII GGWCC 2 cut(s) 46, 546
BaeGI GKGCMC 1 cut(s) 402
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 2 cut(s) 114, 402
BbsI GAAGAC 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 8 cut(s) 24, 65, 71, 78, 171, 372, 394, 464
BccI CCATC 6 cut(s) 46, 303, 340, 410, 472, 484
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 550
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 1 cut(s) 591
BfmI CTRYAG 2 cut(s) 157, 187
BglII AGATCT 1 cut(s) 140
BisI GCNGC 8 cut(s) 13, 60, 67, 79, 160, 383, 386, 453
BlsI GCNGC 8 cut(s) 14, 61, 68, 80, 161, 384, 387, 454
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 1 cut(s) 550
Bme18I GGWCC 2 cut(s) 46, 546
BmgT120I GGNCC 4 cut(s) 46, 398, 399, 546
BmiI GGNNCC 2 cut(s) 206, 400
BmrFI CCNGG 1 cut(s) 550
BpiI GAAGAC 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 429
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 220, 510
Bse1I ACTGG 1 cut(s) 494
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 1 cut(s) 550
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 658
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 124, 220, 510
BseMII CTCAG 2 cut(s) 158, 171
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 2 cut(s) 238, 253
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 8 cut(s) 24, 65, 71, 78, 171, 372, 394, 464
BsgI GTGCAG 1 cut(s) 474
BshFI GGCC 1 cut(s) 400
BshNI GGYRCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 114
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 3 cut(s) 124, 220, 510
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 1 cut(s) 400
Bsp120I GGGCCC 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 114, 402
Bsp143I GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
BspACI CCGC 1 cut(s) 370
BspANI GGCC 1 cut(s) 400
BspCNI CTCAG 2 cut(s) 157, 172
BspLI GGNNCC 2 cut(s) 206, 400
BspMAI CTGCAG 2 cut(s) 161, 191
BspPI GGATC 2 cut(s) 521, 611
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 494
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 550
Bst4CI ACNGT 1 cut(s) 561
Bst6I CTCTTC 1 cut(s) 119
BstAPI GCANNNNNTGC 3 cut(s) 129, 195, 204
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 2 cut(s) 144, 180
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 1 cut(s) 658
BstKTI GATC 7 cut(s) 143, 289, 312, 419, 427, 516, 606
BstMBI GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
BstMWI GCNNNNNNNGC 4 cut(s) 75, 129, 195, 204
BstNI CCWGG 1 cut(s) 550
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 548
BstSFI CTRYAG 2 cut(s) 157, 187
BstSLI GKGCMC 1 cut(s) 402
BstV1I GCAGC 8 cut(s) 24, 65, 71, 78, 171, 372, 394, 464
BstV2I GAAGAC 1 cut(s) 610
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
BsuRI GGCC 1 cut(s) 400
BtsCI GGATG 1 cut(s) 658
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 64
CaiI CAGNNNCTG 1 cut(s) 156
Cfr13I GGNCC 4 cut(s) 46, 398, 399, 546
Csp6I GTAC 3 cut(s) 154, 375, 594
CviAII CATG 2 cut(s) 82, 267
CviJI RGCY 7 cut(s) 69, 99, 112, 385, 400, 539, 646
CviKI_1 RGCY 7 cut(s) 69, 99, 112, 385, 400, 539, 646
CviQI GTAC 3 cut(s) 154, 375, 594
DdeI CTNAG 2 cut(s) 144, 180
DpnI GATC 7 cut(s) 142, 288, 311, 418, 426, 515, 605
DpnII GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 49
Eco24I GRGCYC 2 cut(s) 114, 402
Eco47I GGWCC 2 cut(s) 46, 546
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 604
EcoICRI GAGCTC 1 cut(s) 112
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 548
EcoT14I CCWWGG 1 cut(s) 49
EcoT38I GRGCYC 2 cut(s) 114, 402
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 85, 270
FaiI YATR 6 cut(s) 83, 108, 268, 380, 572, 581
FaqI GGGAC 1 cut(s) 223
FatI CATG 2 cut(s) 81, 266
FauI CCCGC 1 cut(s) 363
Fnu4HI GCNGC 8 cut(s) 13, 60, 67, 79, 160, 383, 386, 453
FokI GGATG 1 cut(s) 665
FriOI GRGCYC 2 cut(s) 114, 402
Fsp4HI GCNGC 8 cut(s) 13, 60, 67, 79, 160, 383, 386, 453
FspBI CTAG 1 cut(s) 591
GluI GCNGC 8 cut(s) 13, 60, 67, 79, 160, 383, 386, 453
HaeIII GGCC 1 cut(s) 400
Hin1II CATG 2 cut(s) 85, 270
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 1 cut(s) 182
HphI GGTGA 5 cut(s) 202, 304, 425, 459, 465
Hpy188I TCNGA 3 cut(s) 140, 565, 635
Hpy188III TCNNGA 4 cut(s) 290, 406, 622, 650
HpyAV CCTTC 3 cut(s) 211, 446, 480
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4V TGCA 9 cut(s) 81, 123, 159, 189, 198, 270, 382, 388, 491
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 129, 195, 204
HpyF3I CTNAG 2 cut(s) 144, 180
Hsp92II CATG 2 cut(s) 85, 270
Kzo9I GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
LguI GCTCTTC 1 cut(s) 119
Lsp1109I GCAGC 8 cut(s) 24, 65, 71, 78, 171, 372, 394, 464
MaeI CTAG 1 cut(s) 591
MaeIII GTNAC 1 cut(s) 499
MalI GATC 7 cut(s) 142, 288, 311, 418, 426, 515, 605
MboI GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
MboII GAAGA 5 cut(s) 106, 249, 419, 610, 648
MfeI CAATTG 1 cut(s) 199
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 2 cut(s) 114, 402
MluCI AATT 5 cut(s) 134, 192, 199, 253, 300
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 9 cut(s) 214, 218, 259, 271, 274, 504, 529, 546, 559
MspA1I CMGCKG 1 cut(s) 385
MspR9I CCNGG 1 cut(s) 550
MunI CAATTG 1 cut(s) 199
MvaI CCWGG 1 cut(s) 550
MwoI GCNNNNNNNGC 4 cut(s) 75, 129, 195, 204
NdeII GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
NlaIII CATG 2 cut(s) 85, 270
NlaIV GGNNCC 2 cut(s) 206, 400
NspI RCATGY 1 cut(s) 85
PciSI GCTCTTC 1 cut(s) 119
PflFI GACNNNGTC 1 cut(s) 559
PkrI GCNGC 8 cut(s) 14, 61, 68, 80, 161, 384, 387, 454
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 548
PspGI CCWGG 1 cut(s) 548
PspN4I GGNNCC 2 cut(s) 206, 400
PspOMI GGGCCC 1 cut(s) 398
PspPI GGNCC 4 cut(s) 46, 398, 399, 546
PstI CTGCAG 2 cut(s) 161, 191
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 1 cut(s) 140
PsyI GACNNNGTC 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 385
RsaI GTAC 3 cut(s) 155, 376, 595
RsaNI GTAC 3 cut(s) 154, 375, 594
SacI GAGCTC 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 119
SatI GCNGC 8 cut(s) 13, 60, 67, 79, 160, 383, 386, 453
Sau3AI GATC 7 cut(s) 140, 286, 309, 416, 424, 513, 603
Sau96I GGNCC 4 cut(s) 46, 398, 399, 546
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 550
SduI GDGCHC 2 cut(s) 114, 402
SetI ASST 8 cut(s) 114, 129, 155, 387, 438, 472, 633, 648
SfcI CTRYAG 2 cut(s) 157, 187
SinI GGWCC 2 cut(s) 46, 546
SpeI ACTAGT 1 cut(s) 590
Sse9I AATT 5 cut(s) 134, 192, 199, 253, 300
SsiI CCGC 1 cut(s) 370
SspMI CTAG 1 cut(s) 591
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 548
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 561
TasI AATT 5 cut(s) 134, 192, 199, 253, 300
TatI WGTACW 1 cut(s) 593
TscAI CASTG 1 cut(s) 48
TseI GCWGC 8 cut(s) 12, 59, 66, 78, 159, 382, 385, 452
TspDTI ATGAA 4 cut(s) 342, 419, 428, 669
TspRI CASTG 1 cut(s) 48
Tth111I GACNNNGTC 1 cut(s) 559
VpaK11BI GGWCC 2 cut(s) 46, 546
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 2 cut(s) 192, 253
XceI RCATGY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XspI CTAG 1 cut(s) 591
ZrmI AGTACT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.