MD15G1381100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012630
Physical Location & Seq
Reverse (-)
46984815 .. 46985978
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1381100.v1.1.491

Sequence Viewer

Length: 663 bp
ATGACAACGAGTAGCAAAGGAAACAACAACGCATCATCAGTAGTCCAAGTCCAAGAGCAAGCAGCAGGCAGCCATGAGTGCTGCATGTGTGGGGATTTTGGCTTCTCCTATGAGCTCTTTGTTTGCAAGGTTTGCCAATTCAGATCTCAGCACAGGTACTGCAGCAATCTTTACCCAAATGCTGAGTCCTACAGAATCTGCAATTGGTGCCTCACCCAGAAGGAGGACACCCAAAACTCCTCAAATTCTTCATCCTCATGCAAAAAGGAATCCCAAGATCAAGACCACCCAATTACCAAGATCAGCATCAAGAAAAAGATTTCATCAAAAAACAGTCATAATCCATATGGTGGGTGTCTGAGGGGCACTATGCAGCTGCAACCAAGCGGGCCCATCAAGAAACAGAGATCACTTGATCTTCATCATCTTCATCGTCAATCATCAGCAGTATCTCCATCACCATCACCATCTCCAAAATCTGCACCAGTTGTTACCAGAAAGAGGATCATCACAAATGCGGCAAAGGAGGCTGAGAGGATCAAGAGGACAAGGTCAGAGGACATATCAAACATAAAAAACACAAGTACTATTGGGATCACAAGGCAAGTCTTCAGGAACAAGGTCAGAAGGTACAAGCTTTTGGATGAAGTTTCAAGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.69

Weight (kDa)

9.65

Isoelectric Point (pI)

60.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD_pln PF25054 28 - 70 1.1e-21 PHD-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 207
AccB7I CCANNNNNTGG 1 cut(s) 350
AciI CCGC 2 cut(s) 387, 518
AclWI GGATC 3 cut(s) 512, 545, 602
AcsI RAATTY 1 cut(s) 244
AcuI CTGAAG 1 cut(s) 595
AfaI GTAC 3 cut(s) 158, 586, 632
AfiI CCNNNNNNNGG 3 cut(s) 223, 350, 501
AgsI TTSAA 1 cut(s) 654
AjuI GAANNNNNNNTTGG 2 cut(s) 187, 219
AluBI AGCT 3 cut(s) 115, 376, 637
AluI AGCT 3 cut(s) 115, 376, 637
Alw21I GWGCWC 1 cut(s) 117
AlwI GGATC 3 cut(s) 512, 545, 602
AlwNI CAGNNNCTG 2 cut(s) 159, 198
AoxI GGCC 1 cut(s) 389
ApaI GGGCCC 1 cut(s) 393
ApeKI GCWGC 6 cut(s) 62, 69, 81, 162, 373, 376
ApoI RAATTY 1 cut(s) 244
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspS9I GGNCC 2 cut(s) 389, 390
AsuHPI GGTGA 3 cut(s) 205, 450, 456
BaeGI GKGCMC 2 cut(s) 368, 393
BanI GGYRCC 1 cut(s) 207
BanII GRGCYC 2 cut(s) 117, 393
BbsI GAAGAC 1 cut(s) 601
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 6 cut(s) 68, 74, 81, 174, 363, 385
BccI CCATC 4 cut(s) 401, 463, 469, 475
BfmI CTRYAG 2 cut(s) 160, 190
BglII AGATCT 1 cut(s) 143
BisI GCNGC 7 cut(s) 63, 70, 82, 163, 374, 377, 519
BlsI GCNGC 7 cut(s) 64, 71, 83, 164, 375, 378, 520
BmcAI AGTACT 1 cut(s) 586
BmgT120I GGNCC 2 cut(s) 389, 390
BmiI GGNNCC 2 cut(s) 209, 391
BmsI GCATC 2 cut(s) 41, 315
BpiI GAAGAC 1 cut(s) 601
BsaBI GATNNNNATC 2 cut(s) 305, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 350, 501
Bse1I ACTGG 1 cut(s) 485
Bse8I GATNNNNATC 2 cut(s) 305, 420
BseGI GGATG 2 cut(s) 251, 649
BseJI GATNNNNATC 2 cut(s) 305, 420
BseLI CCNNNNNNNGG 3 cut(s) 223, 350, 501
BseMII CTCAG 4 cut(s) 161, 174, 350, 522
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 1 cut(s) 229
BseSI GKGCMC 2 cut(s) 368, 393
BseXI GCAGC 6 cut(s) 68, 74, 81, 174, 363, 385
BsgI GTGCAG 1 cut(s) 465
BshFI GGCC 1 cut(s) 391
BshNI GGYRCC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 117
BslI CCNNNNNNNGG 3 cut(s) 223, 350, 501
BsnI GGCC 1 cut(s) 391
Bsp120I GGGCCC 1 cut(s) 389
Bsp1286I GDGCHC 3 cut(s) 117, 368, 393
Bsp143I GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
BspACI CCGC 2 cut(s) 387, 518
BspANI GGCC 1 cut(s) 391
BspCNI CTCAG 4 cut(s) 160, 175, 351, 523
BspLI GGNNCC 2 cut(s) 209, 391
BspMAI CTGCAG 1 cut(s) 164
BspPI GGATC 3 cut(s) 512, 545, 602
BspT107I GGYRCC 1 cut(s) 207
BsrI ACTGG 1 cut(s) 485
BssMI GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
Bst4CI ACNGT 1 cut(s) 335
BstAPI GCANNNNNTGC 2 cut(s) 132, 207
BstC8I GCNNGC 3 cut(s) 60, 67, 389
BstDEI CTNAG 4 cut(s) 147, 183, 359, 531
BstF5I GGATG 2 cut(s) 251, 649
BstKTI GATC 8 cut(s) 146, 280, 303, 410, 418, 507, 540, 597
BstMBI GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
BstMWI GCNNNNNNNGC 4 cut(s) 78, 132, 207, 527
BstNSI RCATGY 1 cut(s) 88
BstSFI CTRYAG 2 cut(s) 160, 190
BstSLI GKGCMC 2 cut(s) 368, 393
BstV1I GCAGC 6 cut(s) 68, 74, 81, 174, 363, 385
BstV2I GAAGAC 1 cut(s) 601
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 1 cut(s) 391
BtsCI GGATG 2 cut(s) 251, 649
Cac8I GCNNGC 3 cut(s) 60, 67, 389
CaiI CAGNNNCTG 2 cut(s) 159, 198
Cfr13I GGNCC 2 cut(s) 389, 390
Csp6I GTAC 3 cut(s) 157, 585, 631
CviAII CATG 3 cut(s) 74, 85, 258
CviJI RGCY 7 cut(s) 72, 102, 115, 376, 391, 530, 637
CviKI_1 RGCY 7 cut(s) 72, 102, 115, 376, 391, 530, 637
CviQI GTAC 3 cut(s) 157, 585, 631
DdeI CTNAG 4 cut(s) 147, 183, 359, 531
DpnI GATC 8 cut(s) 145, 279, 302, 409, 417, 506, 539, 596
DpnII GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
Ecl136II GAGCTC 1 cut(s) 115
Eco24I GRGCYC 2 cut(s) 117, 393
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 595
EcoICRI GAGCTC 1 cut(s) 115
EcoT38I GRGCYC 2 cut(s) 117, 393
FaeI CATG 3 cut(s) 77, 88, 261
FatI CATG 3 cut(s) 73, 84, 257
FauI CCCGC 1 cut(s) 380
FauNDI CATATG 1 cut(s) 346
Fnu4HI GCNGC 7 cut(s) 63, 70, 82, 163, 374, 377, 519
FokI GGATG 2 cut(s) 238, 656
FriOI GRGCYC 2 cut(s) 117, 393
Fsp4HI GCNGC 7 cut(s) 63, 70, 82, 163, 374, 377, 519
GluI GCNGC 7 cut(s) 63, 70, 82, 163, 374, 377, 519
HaeIII GGCC 1 cut(s) 391
Hin1II CATG 3 cut(s) 77, 88, 261
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 3 cut(s) 185, 195, 269
HphI GGTGA 3 cut(s) 205, 450, 456
Hpy188I TCNGA 4 cut(s) 143, 360, 556, 626
Hpy188III TCNNGA 5 cut(s) 281, 310, 397, 541, 613
HpyAV CCTTC 2 cut(s) 214, 621
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4V TGCA 8 cut(s) 84, 126, 162, 201, 261, 373, 379, 482
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 132, 207, 527
HpyF3I CTNAG 4 cut(s) 147, 183, 359, 531
Hsp92II CATG 3 cut(s) 77, 88, 261
Kzo9I GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
LpnPI CCDG 6 cut(s) 51, 139, 230, 498, 508, 598
Lsp1109I GCAGC 6 cut(s) 68, 74, 81, 174, 363, 385
LweI GCATC 2 cut(s) 41, 315
MaeIII GTNAC 1 cut(s) 490
MalI GATC 8 cut(s) 145, 279, 302, 409, 417, 506, 539, 596
MboI GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
MboII GAAGA 4 cut(s) 240, 410, 419, 601
MfeI CAATTG 1 cut(s) 202
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 3 cut(s) 117, 368, 393
MluCI AATT 4 cut(s) 137, 202, 244, 291
MlyI GAGTC 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 256
MspA1I CMGCKG 1 cut(s) 376
MunI CAATTG 1 cut(s) 202
MwoI GCNNNNNNNGC 4 cut(s) 78, 132, 207, 527
NdeI CATATG 1 cut(s) 346
NdeII GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
NlaIII CATG 3 cut(s) 77, 88, 261
NlaIV GGNNCC 2 cut(s) 209, 391
NspI RCATGY 1 cut(s) 88
PfeI GAWTC 2 cut(s) 195, 269
PflFI GACNNNGTC 1 cut(s) 550
PflMI CCANNNNNTGG 1 cut(s) 350
PkrI GCNGC 7 cut(s) 64, 71, 83, 164, 375, 378, 520
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
Psp124BI GAGCTC 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 209, 391
PspOMI GGGCCC 1 cut(s) 389
PspPI GGNCC 2 cut(s) 389, 390
PstI CTGCAG 1 cut(s) 164
PstNI CAGNNNCTG 2 cut(s) 159, 198
PsuI RGATCY 1 cut(s) 143
PsyI GACNNNGTC 1 cut(s) 550
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 3 cut(s) 158, 586, 632
RsaNI GTAC 3 cut(s) 157, 585, 631
RseI CAYNNNNRTG 1 cut(s) 256
SacI GAGCTC 1 cut(s) 117
SatI GCNGC 7 cut(s) 63, 70, 82, 163, 374, 377, 519
Sau3AI GATC 8 cut(s) 143, 277, 300, 407, 415, 504, 537, 594
Sau96I GGNCC 2 cut(s) 389, 390
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 1 cut(s) 194
SduI GDGCHC 3 cut(s) 117, 368, 393
SetI ASST 8 cut(s) 117, 132, 158, 378, 554, 624, 632, 639
SfaNI GCATC 2 cut(s) 41, 315
SfcI CTRYAG 2 cut(s) 160, 190
SmiMI CAYNNNNRTG 1 cut(s) 256
Sse9I AATT 4 cut(s) 137, 202, 244, 291
SsiI CCGC 2 cut(s) 387, 518
SstI GAGCTC 1 cut(s) 117
TaaI ACNGT 1 cut(s) 335
TasI AATT 4 cut(s) 137, 202, 244, 291
TatI WGTACW 1 cut(s) 584
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 2 cut(s) 195, 269
TseI GCWGC 6 cut(s) 62, 69, 81, 162, 373, 376
TspDTI ATGAA 5 cut(s) 240, 312, 410, 419, 660
Tth111I GACNNNGTC 1 cut(s) 550
Van91I CCANNNNNTGG 1 cut(s) 350
XapI RAATTY 1 cut(s) 244
XceI RCATGY 1 cut(s) 88
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.