Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004911

Physical Location & Seq

Genomic Coordinates Reverse (-)

26957415 .. 26962300

Gene Structure 4886 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1207300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGTCTCCGCCACTGCTTGGTGTTGATGAGGAGGAGGGTCAGAGCGATGTTACATTATTGGCTTCTTCGGGTTCTATGGAGAGTGTATGCCAGAACAGTTTGGAACTGAAGGAGCGAAATTACATGGGATTGTCTGATTGCTCTTCGGTAGACAGTTCGAAGGTCTCTGCTGTCTCTGATGGAAGTAGAAGTAGTCTGCATTTGAAGGCTACAGAACTTAGACTTGGGCTTCCTGGATCCCAGTCTCCTGAGAGAGACTCAGAGGCGAGGGTAATCTCCACTCAACTTGATGAGAAGCCCTTATTCCCGTTGCATCCTTTAAAGGATGGCCACTATTCTTCATTGCAGAAAACAGTTGTTTCGGGAAACAAGAGAGGTTTCTCCGATGCTATGGATGAGTTCTCAGAGGGGAAATATGCTAATTCAGAGGTAAACCTGTTACTGTCACCCAGACCTTCTCCAAACTTTGGACTGAAATCTGGTTCTGCACTTGAGAACCCTGGGACTCAACCACCCAAAACAAAAGAGGTGGCACCAGCAAAGGTAGTACAAGAGAGGCCTCATGCTGTCAACGAAAGCAGACCGAATCATAATGAAAATAGTACTAGCGGTGCACCTGCTTCCAAGGCGCAGGTTGTGGGTTGGCCACCTATAAGATCATTTAGGAAGAACTCATTGGCCACTACATCAAAAAACACTGAGGAAGTAGATGGAAAATCGGGCCCTGGTGCTCTGTTTGTCAAGGTCAGCTTGGATGGTGCACCTTATTTGAGGAAAGTAGATTTGAAAAATTACTCTGCATACCAGGAACTTTCTTCTGCTCTTGAAAAGATGTTCCGCTGTTTTACCATAGGTCAATATGGATCTCATGGAGCTCCAGGCAGAGAGATTAGCGAAAGCAAGCTGAAAGATCTGCTTCATGGCTCAGAATATGTTCTAACTTACGAGGACAAAGATGGTGACTGGATGCTCGTGGGCGATGTTCCTTGGGACATGTTCATTGATACCTGCAAAAGGATGAGGATTATGAAGAGCTCAGATGCCATCGGCCTAGCACCAAGGGCCATGGAAAAGTGCAGGAACAGGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA

Protein Analysis

364

Amino Acids

39.57

Weight (kDa)

6.22

Isoelectric Point (pI)

46.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AUX_IAA	PF02309	70 - 354	2e-88	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22670 AT2G22670 AT2G22670 AT2G22670 AT2G22670
fragaria_vesca	FvH4_5g33790 FvH4_5g33790 FvH4_5g33790 FvH4_5g33790 FvH4_5g33790 FvH4_5g33790 FvH4_5g33790 FvH4_5g33790
malus_domestica	MD08G1207300.v1.1 MD15G1391700.v1.1
prunus_persica	Prupe.1G540700_v2.0.a1
pyrus_communis	pycom08g17960 pycom15g35260
rosa_chinensis	RchiOBHm_Chr7g0233101
rosa_laevigata	RLG00000001299
rosa_multiflora	Rmu_co8377091.1_g000001 Rmu_sc0001362.1_g000019
rosa_roxburghii	Rroxscaffold_3G00227660
rosa_rugosa	Rorug07G0278100 Rorug07G0278200
rosa_samantha	Rh7AG433600 Rh7BG406400 Rh7CG452800 Rh7DG422000
rosa_wichuraiana	Rw7G035740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	625
Acc36I	ACCTGC	3 cut(s)	622, 625, 1016
AccB1I	GGYRCC	1 cut(s)	532
AccB7I	CCANNNNNTGG	2 cut(s)	17, 467
AccI	GTMKAC	1 cut(s)	150
AciI	CCGC	3 cut(s)	8, 609, 838
AclWI	GGATC	3 cut(s)	231, 244, 871
AcoI	YGGCCR	3 cut(s)	328, 644, 678
AcuI	CTGAAG	1 cut(s)	128
AfaI	GTAC	2 cut(s)	549, 604
AfiI	CCNNNNNNNGG	3 cut(s)	17, 467, 1014
AflIII	ACRYGT	1 cut(s)	993
AgsI	TTSAA	3 cut(s)	205, 787, 827
AjnI	CCWGG	5 cut(s)	232, 499, 724, 804, 877
AjuI	GAANNNNNNNTTGG	4 cut(s)	207, 239, 659, 691
AluBI	AGCT	4 cut(s)	750, 875, 904, 1035
AluI	AGCT	4 cut(s)	750, 875, 904, 1035
Alw21I	GWGCWC	5 cut(s)	616, 733, 763, 877, 1037
Alw26I	GTCTC	5 cut(s)	9, 169, 178, 249, 249
Alw44I	GTGCAC	2 cut(s)	612, 759
AlwI	GGATC	3 cut(s)	231, 244, 871
AoxI	GGCC	7 cut(s)	328, 557, 644, 678, 721, 1048, 1062
ApaI	GGGCCC	1 cut(s)	725
ApaLI	GTGCAC	2 cut(s)	612, 759
Asp700I	GAANNNNTTC	1 cut(s)	933
AspLEI	GCGC	1 cut(s)	631
AspS9I	GGNCC	3 cut(s)	721, 722, 1062
AsuHPI	GGTGA	2 cut(s)	438, 971
AsuII	TTCGAA	1 cut(s)	158
BaeGI	GKGCMC	3 cut(s)	616, 725, 763
BalI	TGGCCA	3 cut(s)	330, 646, 680
BamHI	GGATCC	1 cut(s)	236
BanI	GGYRCC	1 cut(s)	532
BanII	GRGCYC	3 cut(s)	725, 877, 1037
BauI	CACGAG	1 cut(s)	971
Bbv12I	GWGCWC	5 cut(s)	616, 733, 763, 877, 1037
BccI	CCATC	6 cut(s)	173, 320, 704, 749, 950, 1052
BciT130I	CCWGG	5 cut(s)	234, 501, 726, 806, 879
BcoDI	GTCTC	5 cut(s)	9, 169, 178, 249, 249
BfaI	CTAG	3 cut(s)	606, 1052, 1090
BfmI	CTRYAG	1 cut(s)	210
BfuAI	ACCTGC	3 cut(s)	622, 625, 1016
BglII	AGATCT	1 cut(s)	910
BmcAI	AGTACT	1 cut(s)	604
Bme1390I	CCNGG	5 cut(s)	234, 501, 726, 806, 879
BmgT120I	GGNCC	3 cut(s)	721, 722, 1062
BmiI	GGNNCC	3 cut(s)	238, 534, 723
BmrFI	CCNGG	5 cut(s)	234, 501, 726, 806, 879
BmrI	ACTGGG	1 cut(s)	235
BmsI	GCATC	4 cut(s)	322, 376, 957, 1030
BmuI	ACTGGG	1 cut(s)	235
BplI	GAGNNNNNCTC	2 cut(s)	242, 274
BpmI	CTGGAG	1 cut(s)	861
Bpu14I	TTCGAA	1 cut(s)	158
BpuEI	CTTGAG	1 cut(s)	512
BsaI	GGTCTC	1 cut(s)	169
BsaJI	CCNNGG	7 cut(s)	499, 500, 624, 724, 986, 1058, 1065
Bsc4I	CCNNNNNNNGG	3 cut(s)	17, 467, 1014
Bse1I	ACTGG	2 cut(s)	241, 968
Bse3DI	GCAATG	1 cut(s)	341
BseBI	CCWGG	5 cut(s)	234, 501, 726, 806, 879
BseDI	CCNNGG	7 cut(s)	499, 500, 624, 724, 986, 1058, 1065
BseGI	GGATG	6 cut(s)	313, 331, 400, 760, 972, 1023
BseLI	CCNNNNNNNGG	3 cut(s)	17, 467, 1014
BseMI	GCAATG	1 cut(s)	341
BseMII	CTCAG	6 cut(s)	240, 273, 417, 690, 939, 1050
BseNI	ACTGG	2 cut(s)	241, 968
BseRI	GAGGAG	2 cut(s)	44, 47
BseSI	GKGCMC	3 cut(s)	616, 725, 763
BsgI	GTGCAG	1 cut(s)	471
BshFI	GGCC	7 cut(s)	330, 559, 646, 680, 723, 1050, 1064
BshNI	GGYRCC	1 cut(s)	532
BsiHKAI	GWGCWC	5 cut(s)	616, 733, 763, 877, 1037
BslFI	GGGAC	2 cut(s)	517, 1004
BslI	CCNNNNNNNGG	3 cut(s)	17, 467, 1014
BsmAI	GTCTC	5 cut(s)	9, 169, 178, 249, 249
BsmFI	GGGAC	2 cut(s)	517, 1004
BsnI	GGCC	7 cut(s)	330, 559, 646, 680, 723, 1050, 1064
Bso31I	GGTCTC	1 cut(s)	169
Bsp119I	TTCGAA	1 cut(s)	158
Bsp120I	GGGCCC	1 cut(s)	721
Bsp1286I	GDGCHC	6 cut(s)	616, 725, 733, 763, 877, 1037
Bsp143I	GATC	4 cut(s)	236, 656, 863, 910
Bsp19I	CCATGG	1 cut(s)	1065
BspACI	CCGC	3 cut(s)	8, 609, 838
BspANI	GGCC	7 cut(s)	330, 559, 646, 680, 723, 1050, 1064
BspCNI	CTCAG	6 cut(s)	241, 272, 416, 691, 938, 1049
BspLI	GGNNCC	3 cut(s)	238, 534, 723
BspMI	ACCTGC	3 cut(s)	622, 625, 1016
BspPI	GGATC	3 cut(s)	231, 244, 871
BspQI	GCTCTTC	2 cut(s)	148, 1025
BspT104I	TTCGAA	1 cut(s)	158
BspT107I	GGYRCC	1 cut(s)	532
BspTNI	GGTCTC	1 cut(s)	169
BsrDI	GCAATG	1 cut(s)	341
BsrI	ACTGG	2 cut(s)	241, 968
BssECI	CCNNGG	7 cut(s)	499, 500, 624, 724, 986, 1058, 1065
BssMI	GATC	4 cut(s)	236, 656, 863, 910
BssSI	CACGAG	1 cut(s)	971
BssT1I	CCWWGG	4 cut(s)	624, 986, 1058, 1065
Bst2BI	CACGAG	1 cut(s)	971
Bst2UI	CCWGG	5 cut(s)	234, 501, 726, 806, 879
Bst4CI	ACNGT	4 cut(s)	98, 155, 355, 444
Bst6I	CTCTTC	2 cut(s)	148, 1025
BstBI	TTCGAA	1 cut(s)	158
BstC8I	GCNNGC	1 cut(s)	902
BstDEI	CTNAG	7 cut(s)	218, 249, 259, 403, 699, 925, 1036
BstDSI	CCRYGG	1 cut(s)	1065
BstENI	CCTNNNNNAGG	1 cut(s)	1012
BstF5I	GGATG	6 cut(s)	313, 331, 400, 760, 972, 1023
BstHHI	GCGC	1 cut(s)	631
BstKTI	GATC	4 cut(s)	239, 659, 866, 913
BstMAI	GTCTC	5 cut(s)	9, 169, 178, 249, 249
BstMBI	GATC	4 cut(s)	236, 656, 863, 910
BstMWI	GCNNNNNNNGC	2 cut(s)	626, 1061
BstNI	CCWGG	5 cut(s)	234, 501, 726, 806, 879
BstNSI	RCATGY	1 cut(s)	997
BstSCI	CCNGG	5 cut(s)	232, 499, 724, 804, 877
BstSFI	CTRYAG	1 cut(s)	210
BstSLI	GKGCMC	3 cut(s)	616, 725, 763
BstX2I	RGATCY	3 cut(s)	236, 863, 910
BstYI	RGATCY	3 cut(s)	236, 863, 910
BsuRI	GGCC	7 cut(s)	330, 559, 646, 680, 723, 1050, 1064
BtgI	CCRYGG	1 cut(s)	1065
BtgZI	GCGATG	2 cut(s)	60, 993
BtsCI	GGATG	6 cut(s)	313, 331, 400, 760, 972, 1023
BtsI	GCAGTG	1 cut(s)	11
BtsIMutI	CAGTG	2 cut(s)	11, 696
BveI	ACCTGC	3 cut(s)	622, 625, 1016
Cac8I	GCNNGC	1 cut(s)	902
CfoI	GCGC	1 cut(s)	631
Cfr13I	GGNCC	3 cut(s)	721, 722, 1062
Csp6I	GTAC	2 cut(s)	548, 603
CspCI	CAANNNNNGTGG	4 cut(s)	268, 303, 510, 545
CviAII	CATG	6 cut(s)	124, 563, 869, 920, 994, 1066
CviQI	GTAC	2 cut(s)	548, 603
DdeI	CTNAG	7 cut(s)	218, 249, 259, 403, 699, 925, 1036
DpnI	GATC	4 cut(s)	238, 658, 865, 912
DpnII	GATC	4 cut(s)	236, 656, 863, 910
DraI	TTTAAA	1 cut(s)	321
EaeI	YGGCCR	3 cut(s)	328, 644, 678
Eam1104I	CTCTTC	2 cut(s)	148, 1025
EarI	CTCTTC	2 cut(s)	148, 1025
Ecl136II	GAGCTC	2 cut(s)	875, 1035
Eco130I	CCWWGG	4 cut(s)	624, 986, 1058, 1065
Eco147I	AGGCCT	1 cut(s)	559
Eco24I	GRGCYC	3 cut(s)	725, 877, 1037
Eco31I	GGTCTC	1 cut(s)	169
Eco53kI	GAGCTC	2 cut(s)	875, 1035
Eco57I	CTGAAG	1 cut(s)	128
EcoICRI	GAGCTC	2 cut(s)	875, 1035
EcoNI	CCTNNNNNAGG	1 cut(s)	1012
EcoO109I	RGGNCCY	1 cut(s)	722
EcoRII	CCWGG	5 cut(s)	232, 499, 724, 804, 877
EcoT14I	CCWWGG	4 cut(s)	624, 986, 1058, 1065
EcoT38I	GRGCYC	3 cut(s)	725, 877, 1037
ErhI	CCWWGG	4 cut(s)	624, 986, 1058, 1065
FaeI	CATG	6 cut(s)	127, 566, 872, 923, 997, 1069
FalI	AAGNNNNNCTT	4 cut(s)	734, 766, 900, 932
FaqI	GGGAC	2 cut(s)	517, 1004
FatI	CATG	6 cut(s)	123, 562, 868, 919, 993, 1065
FblI	GTMKAC	1 cut(s)	150
FokI	GGATG	6 cut(s)	300, 338, 407, 767, 979, 1030
FriOI	GRGCYC	3 cut(s)	725, 877, 1037
FspBI	CTAG	3 cut(s)	606, 1052, 1090
GlaI	GCGC	1 cut(s)	630
GsuI	CTGGAG	1 cut(s)	861
HaeIII	GGCC	7 cut(s)	330, 559, 646, 680, 723, 1050, 1064
HhaI	GCGC	1 cut(s)	631
Hin1II	CATG	6 cut(s)	127, 566, 872, 923, 997, 1069
Hin6I	GCGC	1 cut(s)	629
HinP1I	GCGC	1 cut(s)	629
HincII	GTYRAC	1 cut(s)	571
HindII	GTYRAC	1 cut(s)	571
HinfI	GANTC	3 cut(s)	257, 505, 586
HphI	GGTGA	2 cut(s)	438, 971
Hpy166II	GTNNAC	5 cut(s)	151, 433, 571, 614, 761
Hpy188I	TCNGA	9 cut(s)	42, 136, 178, 262, 385, 406, 427, 928, 1039
Hpy188III	TCNNGA	3 cut(s)	248, 363, 824
Hpy8I	GTNNAC	5 cut(s)	151, 433, 571, 614, 761
HpyAV	CCTTC	4 cut(s)	103, 154, 199, 465
HpyCH4III	ACNGT	4 cut(s)	98, 155, 355, 444
HpyCH4V	TGCA	9 cut(s)	199, 313, 346, 488, 614, 761, 800, 1011, 1077
HpyF10VI	GCNNNNNNNGC	2 cut(s)	626, 1061
HpyF3I	CTNAG	7 cut(s)	218, 249, 259, 403, 699, 925, 1036
Hsp92II	CATG	6 cut(s)	127, 566, 872, 923, 997, 1069
HspAI	GCGC	1 cut(s)	629
Kzo9I	GATC	4 cut(s)	236, 656, 863, 910
LguI	GCTCTTC	2 cut(s)	148, 1025
LmnI	GCTCC	3 cut(s)	112, 872, 880
LweI	GCATC	4 cut(s)	322, 376, 957, 1030
MaeI	CTAG	3 cut(s)	606, 1052, 1090
MaeIII	GTNAC	4 cut(s)	49, 438, 444, 959
MalI	GATC	4 cut(s)	238, 658, 865, 912
MboI	GATC	4 cut(s)	236, 656, 863, 910
MboII	GAAGA	6 cut(s)	57, 135, 330, 679, 807, 1042
MflI	RGATCY	3 cut(s)	236, 863, 910
MhlI	GDGCHC	6 cut(s)	616, 725, 733, 763, 877, 1037
MlsI	TGGCCA	3 cut(s)	330, 646, 680
MluCI	AATT	3 cut(s)	118, 421, 790
MluNI	TGGCCA	3 cut(s)	330, 646, 680
MlyI	GAGTC	2 cut(s)	251, 499
Mox20I	TGGCCA	3 cut(s)	330, 646, 680
MroXI	GAANNNNTTC	1 cut(s)	933
MscI	TGGCCA	3 cut(s)	330, 646, 680
MseI	TTAA	1 cut(s)	320
Msp20I	TGGCCA	3 cut(s)	330, 646, 680
MspA1I	CMGCKG	1 cut(s)	840
MspR9I	CCNGG	5 cut(s)	234, 501, 726, 806, 879
MvaI	CCWGG	5 cut(s)	234, 501, 726, 806, 879
MwoI	GCNNNNNNNGC	2 cut(s)	626, 1061
NcoI	CCATGG	1 cut(s)	1065
NdeII	GATC	4 cut(s)	236, 656, 863, 910
NlaIII	CATG	6 cut(s)	127, 566, 872, 923, 997, 1069
NlaIV	GGNNCC	3 cut(s)	238, 534, 723
NmuCI	GTSAC	2 cut(s)	444, 959
NspI	RCATGY	1 cut(s)	997
NspV	TTCGAA	1 cut(s)	158
PaqCI	CACCTGC	1 cut(s)	625
PasI	CCCWGGG	1 cut(s)	500
PceI	AGGCCT	1 cut(s)	559
PciI	ACATGT	1 cut(s)	993
PciSI	GCTCTTC	2 cut(s)	148, 1025
PdmI	GAANNNNTTC	1 cut(s)	933
PfeI	GAWTC	1 cut(s)	586
PflMI	CCANNNNNTGG	2 cut(s)	17, 467
PfoI	TCCNGGA	1 cut(s)	232
PleI	GAGTC	2 cut(s)	251, 499
PpsI	GAGTC	2 cut(s)	251, 499
PscI	ACATGT	1 cut(s)	993
Psp124BI	GAGCTC	2 cut(s)	877, 1037
Psp6I	CCWGG	5 cut(s)	232, 499, 724, 804, 877
PspGI	CCWGG	5 cut(s)	232, 499, 724, 804, 877
PspN4I	GGNNCC	3 cut(s)	238, 534, 723
PspOMI	GGGCCC	1 cut(s)	721
PspPI	GGNCC	3 cut(s)	721, 722, 1062
PsuI	RGATCY	3 cut(s)	236, 863, 910
RsaI	GTAC	2 cut(s)	549, 604
RsaNI	GTAC	2 cut(s)	548, 603
SacI	GAGCTC	2 cut(s)	877, 1037
SapI	GCTCTTC	2 cut(s)	148, 1025
SaqAI	TTAA	1 cut(s)	320
Sau3AI	GATC	4 cut(s)	236, 656, 863, 910
Sau96I	GGNCC	3 cut(s)	721, 722, 1062
ScaI	AGTACT	1 cut(s)	604
SchI	GAGTC	2 cut(s)	251, 499
ScrFI	CCNGG	5 cut(s)	234, 501, 726, 806, 879
SduI	GDGCHC	6 cut(s)	616, 725, 733, 763, 877, 1037
SfaNI	GCATC	4 cut(s)	322, 376, 957, 1030
SfcI	CTRYAG	1 cut(s)	210
SfuI	TTCGAA	1 cut(s)	158
SmlI	CTYRAG	1 cut(s)	491
SmoI	CTYRAG	1 cut(s)	491
Sse9I	AATT	3 cut(s)	118, 421, 790
SseBI	AGGCCT	1 cut(s)	559
SsiI	CCGC	3 cut(s)	8, 609, 838
SspMI	CTAG	3 cut(s)	606, 1052, 1090
SstI	GAGCTC	2 cut(s)	877, 1037
StuI	AGGCCT	1 cut(s)	559
StyD4I	CCNGG	5 cut(s)	232, 499, 724, 804, 877
StyI	CCWWGG	4 cut(s)	624, 986, 1058, 1065
TaaI	ACNGT	4 cut(s)	98, 155, 355, 444
TaqI	TCGA	1 cut(s)	158
TaqII	GACCGA	1 cut(s)	598
TasI	AATT	3 cut(s)	118, 421, 790
TatI	WGTACW	2 cut(s)	547, 602
TfiI	GAWTC	1 cut(s)	586
Tru1I	TTAA	1 cut(s)	320
Tru9I	TTAA	1 cut(s)	320
TscAI	CASTG	2 cut(s)	18, 703
TseFI	GTSAC	2 cut(s)	444, 959
Tsp45I	GTSAC	2 cut(s)	444, 959
TspDTI	ATGAA	5 cut(s)	330, 609, 908, 988, 1043
TspRI	CASTG	2 cut(s)	18, 703
Van91I	CCANNNNNTGG	2 cut(s)	17, 467
VneI	GTGCAC	2 cut(s)	612, 759
XagI	CCTNNNNNAGG	1 cut(s)	1012
XceI	RCATGY	1 cut(s)	997
XmiI	GTMKAC	1 cut(s)	150
XmnI	GAANNNNTTC	1 cut(s)	933
XspI	CTAG	3 cut(s)	606, 1052, 1090
ZrmI	AGTACT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.