Rh7AG433600

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004911
Physical Location & Seq
Reverse (-)
58385722 .. 58389655
3934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG433600.1

Sequence Viewer

Length: 1071 bp
ATGTCACAACCACTTCTCGGTGCTGGGGAGGAGGAAGGTAGGAGTGATGTTACAGCATTATCTTCCTCACTTTCTATGGAGAGTGTATGTCCTAACAGCTCAGATTTGAAGGAGCGAAATTACATGGGATTATCTGATTGCTCTTCGGTAGACAGTTCAAAAGTCTCCCATGTAGATGGAAGTAAAAGTAGACTGAACCTGAAGGCTACGGAACTTAGGCTTGGGCTTCCTGGATCCCAGTCTCCTGACAGAGACTCAGAACTGAGGCTAGTCTCCACTGAAGTTGATGAGAAACCCCTTTTCCCATTACATCCTTTAAAGGATTCATTGCAGAAAACAGTTGTTTCAGGCAACAAGAGAGGGTTCTCTGATGCTATGGATGAGTTCTCAGAGGGCAAATATGCTAATACAGAGGTAAACATGTTGCTGTCACCCAGGCCTTCTCCAAACTTGGGATTGAAAACTGGTTCTGTACTTGAGAACCTTGGGAGTCAGGAGCCCAAAATGAAAGAGATAGCACCACCAAAGATAATGCAAGAGAGGCCTCATGCTGCCAAAGAAACTAGGCCAAATCATTCTAGTACTAACAGTGCACCTGCTACCAAGGCGCAGGTTGTTGGTTGGCCACCTATAAGATCATTTAGGAAGAACTCATTGGCCACTGTCTCAAAGAACGTGGAGGAAGTAGATGGAAAAGCAGGGCCTGGTGCTCTATATGTCAAAGTCAGCATGGATGGTGCTCCTTATTTGAGGAAAGTAGATTTGAAACATTACTCTGCATACCAGGAACTTTCTTCTGCTCTTGAAAAGATGTTTAGCTGTTTTACTATAGGTCAATATGGATCTCATGGAGCTCTGGGCAGAGAGATTAGTGAAAGCAAGCTGAAGGATCTCCTTCATGGCTCAGAATATGTCCTCACCTACGAGGACAAAGATGGTGACTGGATGCTTGTGGGTGATGTCCCTTGGGAGATGTTTATCGATACCTGCAAAAGGATGAGGATAATGAAAAGCTCAGATGCCATTGGCCTTGCTCCAAGAGCCATGGAGAAGTGCAGGAACAGGAACTAG

Protein Analysis

356

Amino Acids

39.06

Weight (kDa)

6.68

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 69 - 347 2.3e-88 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
Acc36I ACCTGC 3 cut(s) 601, 604, 995
AccI GTMKAC 2 cut(s) 150, 190
AclWI GGATC 4 cut(s) 228, 241, 850, 897
AcoI YGGCCR 2 cut(s) 623, 657
AcuI CTGAAG 3 cut(s) 221, 300, 905
AfaI GTAC 2 cut(s) 474, 583
AfiI CCNNNNNNNGG 3 cut(s) 17, 452, 993
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 5 cut(s) 109, 159, 460, 766, 806
AjnI CCWGG 4 cut(s) 229, 434, 703, 783
AjuI GAANNNNNNNTTGG 4 cut(s) 204, 236, 638, 670
AluBI AGCT 5 cut(s) 99, 819, 854, 883, 1014
AluI AGCT 5 cut(s) 99, 819, 854, 883, 1014
Alw21I GWGCWC 4 cut(s) 595, 712, 742, 856
Alw26I GTCTC 5 cut(s) 169, 246, 246, 277, 670
Alw44I GTGCAC 1 cut(s) 591
AlwI GGATC 4 cut(s) 228, 241, 850, 897
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 7 cut(s) 437, 542, 566, 623, 657, 701, 1027
ApaLI GTGCAC 1 cut(s) 591
ApeKI GCWGC 1 cut(s) 551
AspLEI GCGC 1 cut(s) 610
AspS9I GGNCC 1 cut(s) 701
AsuHPI GGTGA 4 cut(s) 423, 910, 950, 968
BaeGI GKGCMC 1 cut(s) 595
BalI TGGCCA 2 cut(s) 625, 659
BamHI GGATCC 1 cut(s) 233
BanII GRGCYC 2 cut(s) 501, 856
Bbv12I GWGCWC 4 cut(s) 595, 712, 742, 856
BbvI GCAGC 1 cut(s) 538
BccI CCATC 4 cut(s) 170, 683, 728, 929
BciT130I CCWGG 4 cut(s) 231, 436, 705, 785
BcoDI GTCTC 5 cut(s) 169, 246, 246, 277, 670
BfaI CTAG 4 cut(s) 269, 564, 579, 1069
BfmI CTRYAG 1 cut(s) 828
BfuAI ACCTGC 3 cut(s) 601, 604, 995
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
BmcAI AGTACT 1 cut(s) 583
Bme1390I CCNGG 4 cut(s) 231, 436, 705, 785
BmgT120I GGNCC 1 cut(s) 701
BmiI GGNNCC 2 cut(s) 235, 498
BmrFI CCNGG 4 cut(s) 231, 436, 705, 785
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 3 cut(s) 361, 936, 1009
BmuI ACTGGG 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 497
Bsa29I ATCGAT 1 cut(s) 981
BsaBI GATNNNNATC 1 cut(s) 977
BsaJI CCNNGG 5 cut(s) 434, 484, 603, 965, 1044
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 452, 993
Bse1I ACTGG 3 cut(s) 238, 469, 947
Bse3DI GCAATG 1 cut(s) 326
Bse8I GATNNNNATC 1 cut(s) 977
BseBI CCWGG 4 cut(s) 231, 436, 705, 785
BseCI ATCGAT 1 cut(s) 981
BseDI CCNNGG 5 cut(s) 434, 484, 603, 965, 1044
BseGI GGATG 5 cut(s) 310, 385, 739, 951, 1002
BseJI GATNNNNATC 1 cut(s) 977
BseLI CCNNNNNNNGG 3 cut(s) 17, 452, 993
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 6 cut(s) 114, 254, 270, 402, 918, 1029
BseNI ACTGG 3 cut(s) 238, 469, 947
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 595
BseXI GCAGC 1 cut(s) 538
BseYI CCCAGC 1 cut(s) 23
BshFI GGCC 7 cut(s) 439, 544, 568, 625, 659, 703, 1029
BshVI ATCGAT 1 cut(s) 981
BsiHKAI GWGCWC 4 cut(s) 595, 712, 742, 856
BslFI GGGAC 1 cut(s) 947
BslI CCNNNNNNNGG 3 cut(s) 17, 452, 993
BsmAI GTCTC 5 cut(s) 169, 246, 246, 277, 670
BsmFI GGGAC 1 cut(s) 947
BsnI GGCC 7 cut(s) 439, 544, 568, 625, 659, 703, 1029
Bsp1286I GDGCHC 5 cut(s) 501, 595, 712, 742, 856
Bsp143I GATC 4 cut(s) 233, 635, 842, 889
Bsp19I CCATGG 1 cut(s) 1044
BspANI GGCC 7 cut(s) 439, 544, 568, 625, 659, 703, 1029
BspCNI CTCAG 6 cut(s) 113, 255, 269, 401, 917, 1028
BspDI ATCGAT 1 cut(s) 981
BspLI GGNNCC 2 cut(s) 235, 498
BspMI ACCTGC 3 cut(s) 601, 604, 995
BspPI GGATC 4 cut(s) 228, 241, 850, 897
BspQI GCTCTTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 326
BsrI ACTGG 3 cut(s) 238, 469, 947
BssECI CCNNGG 5 cut(s) 434, 484, 603, 965, 1044
BssMI GATC 4 cut(s) 233, 635, 842, 889
BssT1I CCWWGG 4 cut(s) 484, 603, 965, 1044
Bst2UI CCWGG 4 cut(s) 231, 436, 705, 785
Bst4CI ACNGT 4 cut(s) 155, 340, 590, 664
Bst6I CTCTTC 1 cut(s) 148
BstC8I GCNNGC 1 cut(s) 881
BstDEI CTNAG 7 cut(s) 100, 215, 256, 263, 388, 904, 1015
BstDSI CCRYGG 1 cut(s) 1044
BstENI CCTNNNNNAGG 1 cut(s) 991
BstF5I GGATG 5 cut(s) 310, 385, 739, 951, 1002
BstHHI GCGC 1 cut(s) 610
BstKTI GATC 4 cut(s) 236, 638, 845, 892
BstMAI GTCTC 5 cut(s) 169, 246, 246, 277, 670
BstMBI GATC 4 cut(s) 233, 635, 842, 889
BstMWI GCNNNNNNNGC 3 cut(s) 541, 605, 1040
BstNI CCWGG 4 cut(s) 231, 436, 705, 785
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 4 cut(s) 229, 434, 703, 783
BstSFI CTRYAG 1 cut(s) 828
BstSLI GKGCMC 1 cut(s) 595
BstV1I GCAGC 1 cut(s) 538
BstX2I RGATCY 3 cut(s) 233, 842, 889
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 3 cut(s) 233, 842, 889
Bsu15I ATCGAT 1 cut(s) 981
BsuRI GGCC 7 cut(s) 439, 544, 568, 625, 659, 703, 1029
BsuTUI ATCGAT 1 cut(s) 981
BtgI CCRYGG 1 cut(s) 1044
BtsCI GGATG 5 cut(s) 310, 385, 739, 951, 1002
BtsIMutI CAGTG 3 cut(s) 276, 595, 660
BveI ACCTGC 3 cut(s) 601, 604, 995
Cac8I GCNNGC 1 cut(s) 881
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 1 cut(s) 610
Cfr13I GGNCC 1 cut(s) 701
ClaI ATCGAT 1 cut(s) 981
Csp6I GTAC 2 cut(s) 473, 582
CspCI CAANNNNNGTGG 4 cut(s) 265, 300, 657, 692
CviAII CATG 8 cut(s) 124, 170, 421, 548, 730, 848, 899, 1045
CviQI GTAC 2 cut(s) 473, 582
DdeI CTNAG 7 cut(s) 100, 215, 256, 263, 388, 904, 1015
DpnI GATC 4 cut(s) 235, 637, 844, 891
DpnII GATC 4 cut(s) 233, 635, 842, 889
DraI TTTAAA 1 cut(s) 318
EaeI YGGCCR 2 cut(s) 623, 657
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 854
Eco130I CCWWGG 4 cut(s) 484, 603, 965, 1044
Eco147I AGGCCT 2 cut(s) 439, 544
Eco24I GRGCYC 2 cut(s) 501, 856
Eco53kI GAGCTC 1 cut(s) 854
Eco57I CTGAAG 3 cut(s) 221, 300, 905
EcoICRI GAGCTC 1 cut(s) 854
EcoNI CCTNNNNNAGG 1 cut(s) 991
EcoO109I RGGNCCY 1 cut(s) 701
EcoRII CCWGG 4 cut(s) 229, 434, 703, 783
EcoT14I CCWWGG 4 cut(s) 484, 603, 965, 1044
EcoT38I GRGCYC 2 cut(s) 501, 856
ErhI CCWWGG 4 cut(s) 484, 603, 965, 1044
FaeI CATG 8 cut(s) 127, 173, 424, 551, 733, 851, 902, 1048
FaqI GGGAC 1 cut(s) 947
FatI CATG 8 cut(s) 123, 169, 420, 547, 729, 847, 898, 1044
FblI GTMKAC 2 cut(s) 150, 190
Fnu4HI GCNGC 1 cut(s) 552
FokI GGATG 5 cut(s) 297, 392, 746, 958, 1009
FriOI GRGCYC 2 cut(s) 501, 856
Fsp4HI GCNGC 1 cut(s) 552
FspBI CTAG 4 cut(s) 269, 564, 579, 1069
GlaI GCGC 1 cut(s) 609
GluI GCNGC 1 cut(s) 552
GsaI CCCAGC 1 cut(s) 27
HaeIII GGCC 7 cut(s) 439, 544, 568, 625, 659, 703, 1029
HhaI GCGC 1 cut(s) 610
Hin1II CATG 8 cut(s) 127, 173, 424, 551, 733, 851, 902, 1048
Hin6I GCGC 1 cut(s) 608
HinP1I GCGC 1 cut(s) 608
HinfI GANTC 3 cut(s) 254, 323, 490
HphI GGTGA 4 cut(s) 423, 910, 950, 968
Hpy166II GTNNAC 4 cut(s) 151, 191, 418, 593
Hpy188I TCNGA 7 cut(s) 103, 136, 259, 370, 391, 907, 1018
Hpy188III TCNNGA 3 cut(s) 245, 494, 803
Hpy8I GTNNAC 4 cut(s) 151, 191, 418, 593
HpyAV CCTTC 6 cut(s) 29, 103, 196, 450, 880, 905
HpyCH4III ACNGT 4 cut(s) 155, 340, 590, 664
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 6 cut(s) 331, 535, 593, 779, 990, 1056
HpyF10VI GCNNNNNNNGC 3 cut(s) 541, 605, 1040
HpyF3I CTNAG 7 cut(s) 100, 215, 256, 263, 388, 904, 1015
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 8 cut(s) 127, 173, 424, 551, 733, 851, 902, 1048
HspAI GCGC 1 cut(s) 608
Kzo9I GATC 4 cut(s) 233, 635, 842, 889
LguI GCTCTTC 1 cut(s) 148
LmnI GCTCC 5 cut(s) 112, 496, 745, 851, 1039
Lsp1109I GCAGC 1 cut(s) 538
LweI GCATC 3 cut(s) 361, 936, 1009
MaeI CTAG 4 cut(s) 269, 564, 579, 1069
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 4 cut(s) 3, 49, 429, 938
MalI GATC 4 cut(s) 235, 637, 844, 891
MboI GATC 4 cut(s) 233, 635, 842, 889
MboII GAAGA 4 cut(s) 54, 135, 658, 786
MflI RGATCY 3 cut(s) 233, 842, 889
MhlI GDGCHC 5 cut(s) 501, 595, 712, 742, 856
MlsI TGGCCA 2 cut(s) 625, 659
MluCI AATT 1 cut(s) 118
MluNI TGGCCA 2 cut(s) 625, 659
MlyI GAGTC 2 cut(s) 248, 499
Mox20I TGGCCA 2 cut(s) 625, 659
MscI TGGCCA 2 cut(s) 625, 659
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 2 cut(s) 625, 659
MspR9I CCNGG 4 cut(s) 231, 436, 705, 785
MvaI CCWGG 4 cut(s) 231, 436, 705, 785
MwoI GCNNNNNNNGC 3 cut(s) 541, 605, 1040
NcoI CCATGG 1 cut(s) 1044
NdeII GATC 4 cut(s) 233, 635, 842, 889
NlaIII CATG 8 cut(s) 127, 173, 424, 551, 733, 851, 902, 1048
NlaIV GGNNCC 2 cut(s) 235, 498
NmuCI GTSAC 3 cut(s) 3, 429, 938
NspI RCATGY 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 604
PceI AGGCCT 2 cut(s) 439, 544
PciI ACATGT 1 cut(s) 420
PciSI GCTCTTC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 229
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 2 cut(s) 248, 498
PpsI GAGTC 2 cut(s) 248, 498
PscI ACATGT 1 cut(s) 420
Psp124BI GAGCTC 1 cut(s) 856
Psp6I CCWGG 4 cut(s) 229, 434, 703, 783
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 4 cut(s) 229, 434, 703, 783
PspN4I GGNNCC 2 cut(s) 235, 498
PspPI GGNCC 1 cut(s) 701
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 3 cut(s) 233, 842, 889
RsaI GTAC 2 cut(s) 474, 583
RsaNI GTAC 2 cut(s) 473, 582
RseI CAYNNNNRTG 1 cut(s) 174
SacI GAGCTC 1 cut(s) 856
SapI GCTCTTC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 4 cut(s) 233, 635, 842, 889
Sau96I GGNCC 1 cut(s) 701
ScaI AGTACT 1 cut(s) 583
SchI GAGTC 2 cut(s) 248, 499
ScrFI CCNGG 4 cut(s) 231, 436, 705, 785
SduI GDGCHC 5 cut(s) 501, 595, 712, 742, 856
SfaNI GCATC 3 cut(s) 361, 936, 1009
SfcI CTRYAG 1 cut(s) 828
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 1 cut(s) 118
SseBI AGGCCT 2 cut(s) 439, 544
SspMI CTAG 4 cut(s) 269, 564, 579, 1069
SstI GAGCTC 1 cut(s) 856
StuI AGGCCT 2 cut(s) 439, 544
StyD4I CCNGG 4 cut(s) 229, 434, 703, 783
StyI CCWWGG 4 cut(s) 484, 603, 965, 1044
TaaI ACNGT 4 cut(s) 155, 340, 590, 664
TaiI ACGT 1 cut(s) 678
TaqI TCGA 1 cut(s) 981
TasI AATT 1 cut(s) 118
TatI WGTACW 2 cut(s) 472, 581
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 3 cut(s) 283, 595, 667
TseFI GTSAC 3 cut(s) 3, 429, 938
TseI GCWGC 1 cut(s) 551
Tsp45I GTSAC 3 cut(s) 3, 429, 938
TspDTI ATGAA 4 cut(s) 315, 521, 887, 1022
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 3 cut(s) 283, 595, 667
VneI GTGCAC 1 cut(s) 591
XagI CCTNNNNNAGG 1 cut(s) 991
XceI RCATGY 1 cut(s) 424
XmiI GTMKAC 2 cut(s) 150, 190
XspI CTAG 4 cut(s) 269, 564, 579, 1069
ZrmI AGTACT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.