Rh7DG422000

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004911
Physical Location & Seq
Reverse (-)
59634010 .. 59640043
6034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG422000.1

Sequence Viewer

Length: 867 bp
ATGTCACAACCACTTCTCGGTGCTGGGGAGGAGGAAGGTAGGAGTGATGTTACAGCATTATCTTCCTCACTTTCTATGGAGAGTGTATGTCCTAACAGCTCAGATTTGAAGGAGCGAAATTACATGGGATTATCTGATTGCTCTTCGGTAGACAGTTCAAAAGTCTCCCATGTAGATGGAAGTAAAAGTAGACTGAACCTGAAGGCTACGGAACTTAGGCTTGGGCTTCCTGGATCCCAGTCTCCTGACAGAGACTCAGAACTGAGGCTAGTCTCCACTAAACTTGATGAGAAACCCCTTTTCCCATTACATCCTTTAAAGGATTCATTGCAGAAAACAGTTGTTTCTGGCAACAAGAGAGGGTTCTCTGATGCTATGGATGAGTTCTCAGAGGGCAAATATGCTAATACAGAGGTAAACATGTTGCTGTCACCCAGGCCTTCTCCAAACTTGGGATTGAAAACTGGTTCTGTACTTGAGAACCTTGGGAGTCAGGAGCCCAAAATGAAAGAGATAGCACCACCAAAGATAATGCAAGAGAGGCCTCATGCTGCCAAAGAAACTAGGCCAAATCATTCTAGTACTAACAGTGCACCTGCTACCAAGGCGCAGGTTGTTGGTTGGCCACCTATAAGATCATTTAGGAAGAACTCATTGGCCACTGTCTCAAAGAACGTGGAGGAAGTAGATGGAAAAGCAGGGCCTGGTGCTCTATATGTCAAAGTCAGCATGGATGGTGCTCCTTATTTGAGGAAAGTAGATTTGAAACATTACTCTGCATACCAGGAACTTTCTTCTGCTCTTGAAAAGATGTTTAGCTGTTTTACTATAGGTGAGCTATTGCCAAGATTCAATTTCTATACATAA

Protein Analysis

288

Amino Acids

31.42

Weight (kDa)

8.29

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 69 - 279 3.8e-46 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
Acc36I ACCTGC 2 cut(s) 601, 604
AccI GTMKAC 2 cut(s) 150, 190
AclWI GGATC 2 cut(s) 228, 241
AcoI YGGCCR 2 cut(s) 623, 657
AcuI CTGAAG 1 cut(s) 221
AfaI GTAC 2 cut(s) 474, 583
AfiI CCNNNNNNNGG 2 cut(s) 17, 452
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 6 cut(s) 109, 159, 460, 766, 806, 853
AjnI CCWGG 4 cut(s) 229, 434, 703, 783
AjuI GAANNNNNNNTTGG 4 cut(s) 204, 236, 638, 670
AluBI AGCT 3 cut(s) 99, 819, 838
AluI AGCT 3 cut(s) 99, 819, 838
Alw21I GWGCWC 3 cut(s) 595, 712, 742
Alw26I GTCTC 5 cut(s) 169, 246, 246, 277, 670
Alw44I GTGCAC 1 cut(s) 591
AlwI GGATC 2 cut(s) 228, 241
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 6 cut(s) 437, 542, 566, 623, 657, 701
ApaLI GTGCAC 1 cut(s) 591
ApeKI GCWGC 1 cut(s) 551
AspLEI GCGC 1 cut(s) 610
AspS9I GGNCC 1 cut(s) 701
AsuHPI GGTGA 2 cut(s) 423, 845
BaeGI GKGCMC 1 cut(s) 595
BalI TGGCCA 2 cut(s) 625, 659
BamHI GGATCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 501
Bbv12I GWGCWC 3 cut(s) 595, 712, 742
BbvI GCAGC 1 cut(s) 538
BccI CCATC 3 cut(s) 170, 683, 728
BciT130I CCWGG 4 cut(s) 231, 436, 705, 785
BcoDI GTCTC 5 cut(s) 169, 246, 246, 277, 670
BfaI CTAG 3 cut(s) 269, 564, 579
BfmI CTRYAG 1 cut(s) 828
BfuAI ACCTGC 2 cut(s) 601, 604
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
BmcAI AGTACT 1 cut(s) 583
Bme1390I CCNGG 4 cut(s) 231, 436, 705, 785
BmgT120I GGNCC 1 cut(s) 701
BmiI GGNNCC 2 cut(s) 235, 498
BmrFI CCNGG 4 cut(s) 231, 436, 705, 785
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 361
BmuI ACTGGG 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 434, 484, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 452
Bse1I ACTGG 2 cut(s) 238, 469
Bse3DI GCAATG 1 cut(s) 326
BseBI CCWGG 4 cut(s) 231, 436, 705, 785
BseDI CCNNGG 3 cut(s) 434, 484, 603
BseGI GGATG 3 cut(s) 310, 385, 739
BseLI CCNNNNNNNGG 2 cut(s) 17, 452
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 4 cut(s) 114, 254, 270, 402
BseNI ACTGG 2 cut(s) 238, 469
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 595
BseXI GCAGC 1 cut(s) 538
BseYI CCCAGC 1 cut(s) 23
BshFI GGCC 6 cut(s) 439, 544, 568, 625, 659, 703
BsiHKAI GWGCWC 3 cut(s) 595, 712, 742
BslI CCNNNNNNNGG 2 cut(s) 17, 452
BsmAI GTCTC 5 cut(s) 169, 246, 246, 277, 670
BsnI GGCC 6 cut(s) 439, 544, 568, 625, 659, 703
Bsp1286I GDGCHC 4 cut(s) 501, 595, 712, 742
Bsp143I GATC 2 cut(s) 233, 635
BspANI GGCC 6 cut(s) 439, 544, 568, 625, 659, 703
BspCNI CTCAG 4 cut(s) 113, 255, 269, 401
BspLI GGNNCC 2 cut(s) 235, 498
BspMI ACCTGC 2 cut(s) 601, 604
BspPI GGATC 2 cut(s) 228, 241
BspQI GCTCTTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 326
BsrI ACTGG 2 cut(s) 238, 469
BssECI CCNNGG 3 cut(s) 434, 484, 603
BssMI GATC 2 cut(s) 233, 635
BssT1I CCWWGG 2 cut(s) 484, 603
Bst2UI CCWGG 4 cut(s) 231, 436, 705, 785
Bst4CI ACNGT 4 cut(s) 155, 340, 590, 664
Bst6I CTCTTC 1 cut(s) 148
BstDEI CTNAG 5 cut(s) 100, 215, 256, 263, 388
BstF5I GGATG 3 cut(s) 310, 385, 739
BstHHI GCGC 1 cut(s) 610
BstKTI GATC 2 cut(s) 236, 638
BstMAI GTCTC 5 cut(s) 169, 246, 246, 277, 670
BstMBI GATC 2 cut(s) 233, 635
BstMWI GCNNNNNNNGC 2 cut(s) 541, 605
BstNI CCWGG 4 cut(s) 231, 436, 705, 785
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 4 cut(s) 229, 434, 703, 783
BstSFI CTRYAG 1 cut(s) 828
BstSLI GKGCMC 1 cut(s) 595
BstV1I GCAGC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 233
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 1 cut(s) 233
BsuRI GGCC 6 cut(s) 439, 544, 568, 625, 659, 703
BtsCI GGATG 3 cut(s) 310, 385, 739
BtsIMutI CAGTG 2 cut(s) 595, 660
BveI ACCTGC 2 cut(s) 601, 604
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 1 cut(s) 610
Cfr13I GGNCC 1 cut(s) 701
Csp6I GTAC 2 cut(s) 473, 582
CspCI CAANNNNNGTGG 4 cut(s) 265, 300, 657, 692
CviAII CATG 5 cut(s) 124, 170, 421, 548, 730
CviQI GTAC 2 cut(s) 473, 582
DdeI CTNAG 5 cut(s) 100, 215, 256, 263, 388
DpnI GATC 2 cut(s) 235, 637
DpnII GATC 2 cut(s) 233, 635
DraI TTTAAA 1 cut(s) 318
EaeI YGGCCR 2 cut(s) 623, 657
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 484, 603
Eco147I AGGCCT 2 cut(s) 439, 544
Eco24I GRGCYC 1 cut(s) 501
Eco57I CTGAAG 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 701
EcoRII CCWGG 4 cut(s) 229, 434, 703, 783
EcoT14I CCWWGG 2 cut(s) 484, 603
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 2 cut(s) 484, 603
FaeI CATG 5 cut(s) 127, 173, 424, 551, 733
FatI CATG 5 cut(s) 123, 169, 420, 547, 729
FblI GTMKAC 2 cut(s) 150, 190
Fnu4HI GCNGC 1 cut(s) 552
FokI GGATG 3 cut(s) 297, 392, 746
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 1 cut(s) 552
FspBI CTAG 3 cut(s) 269, 564, 579
GlaI GCGC 1 cut(s) 609
GluI GCNGC 1 cut(s) 552
GsaI CCCAGC 1 cut(s) 27
HaeIII GGCC 6 cut(s) 439, 544, 568, 625, 659, 703
HhaI GCGC 1 cut(s) 610
Hin1II CATG 5 cut(s) 127, 173, 424, 551, 733
Hin6I GCGC 1 cut(s) 608
HinP1I GCGC 1 cut(s) 608
HinfI GANTC 4 cut(s) 254, 323, 490, 849
HphI GGTGA 2 cut(s) 423, 845
Hpy166II GTNNAC 4 cut(s) 151, 191, 418, 593
Hpy188I TCNGA 5 cut(s) 103, 136, 259, 370, 391
Hpy188III TCNNGA 3 cut(s) 245, 494, 803
Hpy8I GTNNAC 4 cut(s) 151, 191, 418, 593
HpyAV CCTTC 4 cut(s) 29, 103, 196, 450
HpyCH4III ACNGT 4 cut(s) 155, 340, 590, 664
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 4 cut(s) 331, 535, 593, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 541, 605
HpyF3I CTNAG 5 cut(s) 100, 215, 256, 263, 388
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 5 cut(s) 127, 173, 424, 551, 733
HspAI GCGC 1 cut(s) 608
Kzo9I GATC 2 cut(s) 233, 635
LguI GCTCTTC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 112, 496, 745
Lsp1109I GCAGC 1 cut(s) 538
LweI GCATC 1 cut(s) 361
MaeI CTAG 3 cut(s) 269, 564, 579
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 3 cut(s) 3, 49, 429
MalI GATC 2 cut(s) 235, 637
MboI GATC 2 cut(s) 233, 635
MboII GAAGA 4 cut(s) 54, 135, 658, 786
MflI RGATCY 1 cut(s) 233
MhlI GDGCHC 4 cut(s) 501, 595, 712, 742
MlsI TGGCCA 2 cut(s) 625, 659
MluCI AATT 2 cut(s) 118, 853
MluNI TGGCCA 2 cut(s) 625, 659
MlyI GAGTC 2 cut(s) 248, 499
Mox20I TGGCCA 2 cut(s) 625, 659
MscI TGGCCA 2 cut(s) 625, 659
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 2 cut(s) 625, 659
MspR9I CCNGG 4 cut(s) 231, 436, 705, 785
MvaI CCWGG 4 cut(s) 231, 436, 705, 785
MwoI GCNNNNNNNGC 2 cut(s) 541, 605
NdeII GATC 2 cut(s) 233, 635
NlaIII CATG 5 cut(s) 127, 173, 424, 551, 733
NlaIV GGNNCC 2 cut(s) 235, 498
NmuCI GTSAC 2 cut(s) 3, 429
NspI RCATGY 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 604
PceI AGGCCT 2 cut(s) 439, 544
PciI ACATGT 1 cut(s) 420
PciSI GCTCTTC 1 cut(s) 148
PfeI GAWTC 2 cut(s) 323, 849
PfoI TCCNGGA 1 cut(s) 229
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 2 cut(s) 248, 498
PpsI GAGTC 2 cut(s) 248, 498
PscI ACATGT 1 cut(s) 420
Psp6I CCWGG 4 cut(s) 229, 434, 703, 783
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 4 cut(s) 229, 434, 703, 783
PspN4I GGNNCC 2 cut(s) 235, 498
PspPI GGNCC 1 cut(s) 701
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 233
RsaI GTAC 2 cut(s) 474, 583
RsaNI GTAC 2 cut(s) 473, 582
RseI CAYNNNNRTG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 148
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 2 cut(s) 233, 635
Sau96I GGNCC 1 cut(s) 701
ScaI AGTACT 1 cut(s) 583
SchI GAGTC 2 cut(s) 248, 499
ScrFI CCNGG 4 cut(s) 231, 436, 705, 785
SduI GDGCHC 4 cut(s) 501, 595, 712, 742
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 1 cut(s) 828
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 2 cut(s) 118, 853
SseBI AGGCCT 2 cut(s) 439, 544
SspMI CTAG 3 cut(s) 269, 564, 579
StuI AGGCCT 2 cut(s) 439, 544
StyD4I CCNGG 4 cut(s) 229, 434, 703, 783
StyI CCWWGG 2 cut(s) 484, 603
TaaI ACNGT 4 cut(s) 155, 340, 590, 664
TaiI ACGT 1 cut(s) 678
TasI AATT 2 cut(s) 118, 853
TatI WGTACW 2 cut(s) 472, 581
TfiI GAWTC 2 cut(s) 323, 849
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 2 cut(s) 595, 667
TseFI GTSAC 2 cut(s) 3, 429
TseI GCWGC 1 cut(s) 551
Tsp45I GTSAC 2 cut(s) 3, 429
TspDTI ATGAA 2 cut(s) 315, 521
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 2 cut(s) 595, 667
VneI GTGCAC 1 cut(s) 591
XceI RCATGY 1 cut(s) 424
XmiI GTMKAC 2 cut(s) 150, 190
XspI CTAG 3 cut(s) 269, 564, 579
ZrmI AGTACT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.