MD08G1239300.v1.1

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011644
Physical Location & Seq
Reverse (-)
30593260 .. 30596743
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1239300.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGAAGAAGCTCTTGATTTTCACCTTCTCTCTCATCACCTCCTTCTCATTGTCATCTGGGTTCGATCCATTAGATCCATATGCCAACATAACCGTCACTTGGGATTTTTTGCAGCAAAGCGAAAGCACATCTGACATAAAAGTATCGATATACAACTTCCAGCAATTTCGGCATGTGGATCGCCCCGGTTGGAAGCTGGGTTGGAGATGGCAAGGTGATGAAGTGATATGGAACATGTGGGGAGCTGAGGCCATTTATCAAGGAAACTGCTCTCGGTTCAAAGGCGCACCAGAGCGTCCTCATTGCTGTGAAAAGGAGCCGGTGATCATCGATCTCCTCCCCGGAACCCCTTTTAACAAACAGTTCTCCAATTGCTGCAAGGGAGGAGAGCTTTCTTCCATGGTCCAAGACTCTTCCAAGTTTCTATCTGCCTTTCGGATGAATGTTGGAGCATTTTCCAACAATAGTACAAATATAAATATGCCCGACAATTTCACCCTTGGACTCCCTGGTTACACCTGCGGCGCGCCTGTCCAAGTCCAACCGACTAAGACTAGCCAAGATGGCCGGCGATGGACAGAAACTCTCGAGACATGGAATGTGACTTGTATGTACTCCCAGTTCAGAGCATCACCTTCTCCAAAGTGCTGTGTTTCCTTGTCTTCATTCTACAATAGCACAATTGCTAAGTGCCCCCGCTGCAGCTGCGGCTGCCAAGGACTTCCCGGAGCAAAATGCGTGAAGCCAGGTGATGAGTTGCAACTTCCTGGTGAGCGCGAGACTGAAGAACCATCACCTTTGGTGACTTGTACACGACATATGTGCCCGATAGGAATACATTGGCATGTTAAGGAAAGTTACAAGGAGTACTGGAGGGTTAAGATCACAATCACCAATTATAATTTTGTTAAAAACTATTCTGACTGGACCTTAGTCGTTCAACACCCGAATTTGCAAAGTCTAACGCAGTTGTTCAGCTTCAACTACCACCCTCTCGGTCAGTATGGAAATATAAATGATACTGGAATGTTTTGGGGGATCAAATATTACAACGACATGTTACTCAGTACGGGAAAGAATGGGAACGTGCAAACTGAGATGGTACTGCATAAAGATGAAGGGGTTTTCACTTTCAGAGAGGGATGGACTTTTCCCAGGAGAATTTCATTCAACGGGGATGAATGCGTGATGCCCTCTCCTGACGAGTACCCTAGACTTCCCAATAGGGCTCATAGGGCAGCAGCATCAAGGCCATCCATCGTAGTTTTTGCCTCGTTGTTGATCCTTGCAGCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020 GO:0031224 GO:0031225 GO:0044425
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

433

Amino Acids

49.02

Weight (kDa)

8.06

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 47 - 206 1.7e-57 COBRA-like protein
COB_C PF25079 215 - 404 5.4e-48 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011644)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09790 AT1G09790
fragaria_vesca FvH4_1g08930 FvH4_5g28720
malus_domestica MD08G1239300.v1.1 MD15G1430900.v1.1
prunus_persica Prupe.1G575900_v2.0.a1 Prupe.1G575900_v2.0.a1
pyrus_communis pycom08g20760
rosa_chinensis RchiOBHm_Chr7g0220881
rosa_laevigata RLG00000002195 RLG00000015287
rosa_multiflora Rmu_ssc0000286.1_g000004
rosa_roxburghii Rroxscaffold_3G00238910
rosa_rugosa Rorug07G0198100
rosa_samantha Rh7AG338300 Rh7BG329100 Rh7CG356400 Rh7DG337200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 900
AarI CACCTGC 1 cut(s) 527
Acc36I ACCTGC 1 cut(s) 527
AccII CGCG 2 cut(s) 527, 777
AciI CCGC 3 cut(s) 522, 697, 708
AclWI GGATC 5 cut(s) 59, 68, 186, 1046, 1276
AcoI YGGCCR 1 cut(s) 565
AcsI RAATTY 2 cut(s) 949, 1161
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 7 cut(s) 469, 614, 811, 869, 1069, 1104, 1208
AfiI CCNNNNNNNGG 1 cut(s) 99
AflIII ACRYGT 2 cut(s) 234, 1056
AgsI TTSAA 4 cut(s) 280, 941, 982, 1171
AjnI CCWGG 4 cut(s) 508, 745, 766, 1154
AluBI AGCT 7 cut(s) 10, 196, 245, 391, 705, 978, 1292
AluI AGCT 7 cut(s) 10, 196, 245, 391, 705, 978, 1292
Alw26I GTCTC 2 cut(s) 584, 773
AlwI GGATC 5 cut(s) 59, 68, 186, 1046, 1276
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 3 cut(s) 249, 565, 1250
ApeKI GCWGC 9 cut(s) 112, 375, 699, 702, 705, 711, 1238, 1241, 1289
ApoI RAATTY 2 cut(s) 949, 1161
AscI GGCGCGCC 1 cut(s) 525
AspLEI GCGC 4 cut(s) 287, 527, 529, 777
AspS9I GGNCC 2 cut(s) 403, 927
AsuC2I CCSGG 3 cut(s) 186, 342, 726
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 2 cut(s) 403, 927
BaeGI GKGCMC 2 cut(s) 695, 827
BanII GRGCYC 1 cut(s) 1231
BbsI GAAGAC 1 cut(s) 654
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 8 cut(s) 124, 362, 686, 692, 698, 714, 1250, 1253
BccI CCATC 8 cut(s) 201, 557, 567, 799, 1093, 1137, 1261, 1265
BcgI CGANNNNNNTGC 4 cut(s) 161, 195, 804, 838
BciT130I CCWGG 4 cut(s) 510, 747, 768, 1156
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 3 cut(s) 186, 342, 726
BcoDI GTCTC 2 cut(s) 584, 773
BfaI CTAG 2 cut(s) 555, 1212
BfmI CTRYAG 1 cut(s) 700
BfuAI ACCTGC 1 cut(s) 527
BglI GCCNNNNNGGC 1 cut(s) 564
BmcAI AGTACT 1 cut(s) 869
Bme1390I CCNGG 7 cut(s) 186, 342, 510, 726, 747, 768, 1156
Bme18I GGWCC 2 cut(s) 403, 927
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 2 cut(s) 403, 927
BmiI GGNNCC 2 cut(s) 318, 346
BmrFI CCNGG 7 cut(s) 186, 342, 510, 726, 747, 768, 1156
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 3 cut(s) 638, 1179, 1253
BmuI ACTGGG 1 cut(s) 613
BoxI GACNNNNGTC 1 cut(s) 932
BpiI GAAGAC 1 cut(s) 654
BpmI CTGGAG 1 cut(s) 892
Bpu10I CCTNAGC 1 cut(s) 246
BpuMI CCSGG 3 cut(s) 186, 342, 726
Bsa29I ATCGAT 2 cut(s) 146, 330
BsaBI GATNNNNATC 1 cut(s) 887
BsaJI CCNNGG 7 cut(s) 184, 340, 399, 499, 508, 715, 1154
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse118I RCCGGY 2 cut(s) 319, 567
Bse1I ACTGG 4 cut(s) 619, 875, 929, 1027
Bse3DI GCAATG 1 cut(s) 301
Bse8I GATNNNNATC 1 cut(s) 887
BseBI CCWGG 4 cut(s) 510, 747, 768, 1156
BseCI ATCGAT 2 cut(s) 146, 330
BseDI CCNNGG 7 cut(s) 184, 340, 399, 499, 508, 715, 1154
BseGI GGATG 4 cut(s) 444, 1148, 1183, 1253
BseJI GATNNNNATC 1 cut(s) 887
BseLI CCNNNNNNNGG 1 cut(s) 99
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 3 cut(s) 237, 1078, 1086
BseNI ACTGG 4 cut(s) 619, 875, 929, 1027
BsePI GCGCGC 1 cut(s) 525
BseRI GAGGAG 2 cut(s) 326, 399
BseSI GKGCMC 2 cut(s) 695, 827
BseXI GCAGC 8 cut(s) 124, 362, 686, 692, 698, 714, 1250, 1253
BseYI CCCAGC 1 cut(s) 196
Bsh1236I CGCG 2 cut(s) 527, 777
BshFI GGCC 3 cut(s) 251, 567, 1252
BshVI ATCGAT 2 cut(s) 146, 330
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 5 cut(s) 186, 320, 342, 568, 726
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 2 cut(s) 584, 773
BsmI GAATGC 1 cut(s) 1187
BsnI GGCC 3 cut(s) 251, 567, 1252
BsoBI CYCGRG 1 cut(s) 587
Bsp1286I GDGCHC 3 cut(s) 695, 827, 1231
Bsp1407I TGTACA 1 cut(s) 809
Bsp143I GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 3 cut(s) 522, 697, 708
BspANI GGCC 3 cut(s) 251, 567, 1252
BspCNI CTCAG 3 cut(s) 238, 1077, 1087
BspDI ATCGAT 2 cut(s) 146, 330
BspFNI CGCG 2 cut(s) 527, 777
BspLI GGNNCC 2 cut(s) 318, 346
BspMAI CTGCAG 1 cut(s) 704
BspMI ACCTGC 1 cut(s) 527
BspPI GGATC 5 cut(s) 59, 68, 186, 1046, 1276
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 2 cut(s) 319, 567
BsrGI TGTACA 1 cut(s) 809
BsrI ACTGG 4 cut(s) 619, 875, 929, 1027
BssAI RCCGGY 2 cut(s) 319, 567
BssECI CCNNGG 7 cut(s) 184, 340, 399, 499, 508, 715, 1154
BssHII GCGCGC 1 cut(s) 525
BssMI GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
BssT1I CCWWGG 3 cut(s) 399, 499, 715
Bst2UI CCWGG 4 cut(s) 510, 747, 768, 1156
Bst4CI ACNGT 2 cut(s) 94, 363
Bst6I CTCTTC 1 cut(s) 418
BstAUI TGTACA 1 cut(s) 809
BstC8I GCNNGC 2 cut(s) 527, 569
BstDEI CTNAG 6 cut(s) 246, 549, 687, 931, 1064, 1095
BstDSI CCRYGG 1 cut(s) 399
BstF5I GGATG 4 cut(s) 444, 1148, 1183, 1253
BstFNI CGCG 2 cut(s) 527, 777
BstHHI GCGC 4 cut(s) 287, 527, 529, 777
BstKTI GATC 8 cut(s) 67, 76, 181, 327, 334, 885, 1041, 1284
BstMAI GTCTC 2 cut(s) 584, 773
BstMBI GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
BstMWI GCNNNNNNNGC 7 cut(s) 169, 564, 699, 705, 708, 711, 1235
BstNI CCWGG 4 cut(s) 510, 747, 768, 1156
BstNSI RCATGY 4 cut(s) 176, 238, 848, 1060
BstPAI GACNNNNGTC 1 cut(s) 932
BstSCI CCNGG 7 cut(s) 184, 340, 508, 724, 745, 766, 1154
BstSFI CTRYAG 1 cut(s) 700
BstSLI GKGCMC 2 cut(s) 695, 827
BstUI CGCG 2 cut(s) 527, 777
BstV1I GCAGC 8 cut(s) 124, 362, 686, 692, 698, 714, 1250, 1253
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
Bsu15I ATCGAT 2 cut(s) 146, 330
BsuRI GGCC 3 cut(s) 251, 567, 1252
BsuTUI ATCGAT 2 cut(s) 146, 330
BtgI CCRYGG 1 cut(s) 399
BtgZI GCGATG 1 cut(s) 586
BtsCI GGATG 4 cut(s) 444, 1148, 1183, 1253
BveI ACCTGC 1 cut(s) 527
Cac8I GCNNGC 2 cut(s) 527, 569
CfoI GCGC 4 cut(s) 287, 527, 529, 777
Cfr10I RCCGGY 2 cut(s) 319, 567
Cfr13I GGNCC 2 cut(s) 403, 927
ClaI ATCGAT 2 cut(s) 146, 330
CseI GACGC 1 cut(s) 284
Csp6I GTAC 7 cut(s) 468, 613, 810, 868, 1068, 1103, 1207
CviAII CATG 6 cut(s) 173, 235, 400, 594, 845, 1057
CviQI GTAC 7 cut(s) 468, 613, 810, 868, 1068, 1103, 1207
DdeI CTNAG 6 cut(s) 246, 549, 687, 931, 1064, 1095
DpnI GATC 8 cut(s) 66, 75, 180, 326, 333, 884, 1040, 1283
DpnII GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 418
Eco130I CCWWGG 3 cut(s) 399, 499, 715
Eco24I GRGCYC 1 cut(s) 1231
Eco47I GGWCC 2 cut(s) 403, 927
Eco57I CTGAAG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 587
EcoRII CCWGG 4 cut(s) 508, 745, 766, 1154
EcoT14I CCWWGG 3 cut(s) 399, 499, 715
EcoT38I GRGCYC 1 cut(s) 1231
ErhI CCWWGG 3 cut(s) 399, 499, 715
FaeI CATG 6 cut(s) 176, 238, 403, 597, 848, 1060
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 6 cut(s) 172, 234, 399, 593, 844, 1056
FauI CCCGC 1 cut(s) 704
FauNDI CATATG 2 cut(s) 79, 819
FbaI TGATCA 1 cut(s) 324
FokI GGATG 4 cut(s) 451, 1155, 1190, 1240
FriOI GRGCYC 1 cut(s) 1231
FspBI CTAG 2 cut(s) 555, 1212
GlaI GCGC 4 cut(s) 286, 526, 528, 776
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 892
HaeIII GGCC 3 cut(s) 251, 567, 1252
HapII CCGG 5 cut(s) 186, 320, 342, 568, 726
HgaI GACGC 1 cut(s) 284
HhaI GCGC 4 cut(s) 287, 527, 529, 777
Hin1II CATG 6 cut(s) 176, 238, 403, 597, 848, 1060
Hin6I GCGC 4 cut(s) 285, 525, 527, 775
HinP1I GCGC 4 cut(s) 285, 525, 527, 775
HinfI GANTC 2 cut(s) 410, 504
HpaII CCGG 5 cut(s) 186, 320, 342, 568, 726
Hpy166II GTNNAC 1 cut(s) 812
Hpy188I TCNGA 5 cut(s) 133, 438, 626, 922, 1136
Hpy188III TCNNGA 4 cut(s) 13, 587, 589, 1199
Hpy8I GTNNAC 1 cut(s) 812
HpyAV CCTTC 4 cut(s) 34, 52, 645, 1112
HpyCH4III ACNGT 2 cut(s) 94, 363
HpyCH4IV ACGT 1 cut(s) 1086
HpyCH4V TGCA 8 cut(s) 112, 378, 702, 760, 955, 1090, 1108, 1289
HpyF10VI GCNNNNNNNGC 7 cut(s) 169, 564, 699, 705, 708, 711, 1235
HpyF3I CTNAG 6 cut(s) 246, 549, 687, 931, 1064, 1095
HpySE526I ACGT 1 cut(s) 1086
Hsp92II CATG 6 cut(s) 176, 238, 403, 597, 848, 1060
HspAI GCGC 4 cut(s) 285, 525, 527, 775
KroI GCCGGC 1 cut(s) 567
KroNI GCCGGC 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
LmnI GCTCC 4 cut(s) 242, 316, 449, 728
Lsp1109I GCAGC 8 cut(s) 124, 362, 686, 692, 698, 714, 1250, 1253
LweI GCATC 3 cut(s) 638, 1179, 1253
MaeI CTAG 2 cut(s) 555, 1212
MaeII ACGT 1 cut(s) 1086
MaeIII GTNAC 6 cut(s) 94, 512, 601, 802, 857, 1059
MalI GATC 8 cut(s) 66, 75, 180, 326, 333, 884, 1040, 1283
MboI GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
MboII GAAGA 5 cut(s) 16, 387, 405, 654, 797
MfeI CAATTG 2 cut(s) 370, 681
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 3 cut(s) 695, 827, 1231
MluCI AATT 8 cut(s) 164, 370, 490, 681, 895, 901, 949, 1161
MlyI GAGTC 2 cut(s) 404, 498
MmeI TCCRAC 5 cut(s) 170, 182, 427, 483, 565
MroNI GCCGGC 1 cut(s) 567
MseI TTAA 4 cut(s) 354, 849, 879, 909
MslI CAYNNNNRTG 3 cut(s) 306, 843, 1113
MspA1I CMGCKG 2 cut(s) 699, 705
MspI CCGG 5 cut(s) 186, 320, 342, 568, 726
MspR9I CCNGG 7 cut(s) 186, 342, 510, 726, 747, 768, 1156
MunI CAATTG 2 cut(s) 370, 681
Mva1269I GAATGC 1 cut(s) 1187
MvaI CCWGG 4 cut(s) 510, 747, 768, 1156
MvnI CGCG 2 cut(s) 527, 777
MwoI GCNNNNNNNGC 7 cut(s) 169, 564, 699, 705, 708, 711, 1235
NaeI GCCGGC 1 cut(s) 569
NciI CCSGG 3 cut(s) 186, 342, 726
NcoI CCATGG 1 cut(s) 399
NdeI CATATG 2 cut(s) 79, 819
NdeII GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
NgoMIV GCCGGC 1 cut(s) 567
NlaIII CATG 6 cut(s) 176, 238, 403, 597, 848, 1060
NlaIV GGNNCC 2 cut(s) 318, 346
NmuCI GTSAC 3 cut(s) 94, 601, 802
NspI RCATGY 4 cut(s) 176, 238, 848, 1060
PaeR7I CTCGAG 1 cut(s) 587
PalAI GGCGCGCC 1 cut(s) 525
PaqCI CACCTGC 1 cut(s) 527
PauI GCGCGC 1 cut(s) 525
PciI ACATGT 2 cut(s) 234, 1056
PctI GAATGC 1 cut(s) 1187
PdiI GCCGGC 1 cut(s) 569
PfoI TCCNGGA 1 cut(s) 724
PleI GAGTC 2 cut(s) 404, 498
PpsI GAGTC 2 cut(s) 404, 498
PscI ACATGT 2 cut(s) 234, 1056
PshAI GACNNNNGTC 1 cut(s) 932
PsiI TTATAA 1 cut(s) 900
Psp6I CCWGG 4 cut(s) 508, 745, 766, 1154
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 4 cut(s) 508, 745, 766, 1154
PspN4I GGNNCC 2 cut(s) 318, 346
PspPI GGNCC 2 cut(s) 403, 927
PsrI GAACNNNNNNTAC 2 cut(s) 605, 637
PstI CTGCAG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 73
PteI GCGCGC 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 705
RsaI GTAC 7 cut(s) 469, 614, 811, 869, 1069, 1104, 1208
RsaNI GTAC 7 cut(s) 468, 613, 810, 868, 1068, 1103, 1207
RseI CAYNNNNRTG 3 cut(s) 306, 843, 1113
SaqAI TTAA 4 cut(s) 354, 849, 879, 909
Sau3AI GATC 8 cut(s) 64, 73, 178, 324, 331, 882, 1038, 1281
Sau96I GGNCC 2 cut(s) 403, 927
ScaI AGTACT 1 cut(s) 869
SchI GAGTC 2 cut(s) 404, 498
ScrFI CCNGG 7 cut(s) 186, 342, 510, 726, 747, 768, 1156
SduI GDGCHC 3 cut(s) 695, 827, 1231
SfaNI GCATC 3 cut(s) 638, 1179, 1253
SfcI CTRYAG 1 cut(s) 700
Sfr274I CTCGAG 1 cut(s) 587
SgsI GGCGCGCC 1 cut(s) 525
SinI GGWCC 2 cut(s) 403, 927
SlaI CTCGAG 1 cut(s) 587
SmiMI CAYNNNNRTG 3 cut(s) 306, 843, 1113
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 8 cut(s) 164, 370, 490, 681, 895, 901, 949, 1161
SsiI CCGC 3 cut(s) 522, 697, 708
SspI AATATT 1 cut(s) 1046
SspMI CTAG 2 cut(s) 555, 1212
StyD4I CCNGG 7 cut(s) 184, 340, 508, 724, 745, 766, 1154
StyI CCWWGG 3 cut(s) 399, 499, 715
TaaI ACNGT 2 cut(s) 94, 363
TaiI ACGT 1 cut(s) 1089
TaqI TCGA 4 cut(s) 63, 146, 330, 588
TaqII GACCGA 1 cut(s) 986
TasI AATT 8 cut(s) 164, 370, 490, 681, 895, 901, 949, 1161
TatI WGTACW 4 cut(s) 467, 612, 809, 867
TauI GCSGC 2 cut(s) 525, 711
Tru1I TTAA 4 cut(s) 354, 849, 879, 909
Tru9I TTAA 4 cut(s) 354, 849, 879, 909
TseFI GTSAC 3 cut(s) 94, 601, 802
TseI GCWGC 9 cut(s) 112, 375, 699, 702, 705, 711, 1238, 1241, 1289
Tsp45I GTSAC 3 cut(s) 94, 601, 802
TspDTI ATGAA 7 cut(s) 17, 234, 455, 654, 1131, 1155, 1194
VpaK11BI GGWCC 2 cut(s) 403, 927
XapI RAATTY 2 cut(s) 949, 1161
XceI RCATGY 4 cut(s) 176, 238, 848, 1060
XhoI CTCGAG 1 cut(s) 587
XspI CTAG 2 cut(s) 555, 1212
ZrmI AGTACT 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.