MD08G1245000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010894
Physical Location & Seq
Forward (+)
30986312 .. 30989324
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1245000.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGCCCCTGACGAGAATTGCTGCCGATACACTCGGTGTGTTGACCATATGTCTAGTTGCCCTCTTGACCCTTCTTGGTCTCTTCTGCATTATTTACCTGTTTTACTTTCGCTCTCGCATTCGTAATCAGGGTTTTATTCAGCTGAGTTATTTCAGTGGCCCTTGGATTATCCGAATCACTTTCATCTTGTTTGCAATCTGGTGGGGTTTTGGTGAAGTTGTCCGTTTAAGTCTGTTGAGACGTCAGGGGAGAGTGTTGAACGCCCTTAACTTCAAATGGCAGGAGAATCTTTGCAAGTGTTACATTGTCTCAAACCTTGGGTTTGCGGAACCATGCCTATTCCTGACCCTTGTGTTCCTACTTCGTGCGCCTTTGCAGACAATGGAGTCAGGAATTCTAAGCCGAAAGTGGAATGGGAAAACAGCTGGTTACATTCTTTTCTTTTGCCTCCCATTGGTTGCTCTTCAGCTCTTTGTTATTTTAATTGGACCCGAACTCCACAAGAAAGACAGTTTACATAAATTGCCTGACTACTTCACAAGTACAGTGAACAAGACTGATCATGTTGCCTTCTGCACTTACCCTTTGCTGAGCACCATCGTGCTTGGGATTTTTGCCATCATTCTAACTGCCTACTTGTTTTGGCTTGGAAGGCGGATTTTAAAATTGGTCATCAACAAAGGTCTGCAGAAGAGAGTTTATACATTGATATTCTCGGTTTCAAGTTTCCTTCCGTTAAGAGTTACTTTGCTTGGTTTCTCTGTTTTATCTAAACCAGAGCACGTTCAGTTTGAAGCTCTTGTTTTCTTGGCGTTTCTTGCACTTTTATGTTGTGCCGGGGTGTGTATATGCATGCTTGTTTACTGCCCAGTTGCGGATTCTTTAGCCCTTGGGAGTTTACAGGACTTGGAGAGTAGGAGAGTTAATGATGATCAGAATGACACCATTTCTCTCATTGCTAACCAGAGTCATATGGAGGAAAGTTCTGGAATCAGCCCTTGTAGAGACTCCAATGTATCAGCCAAGCGTGGATCAATTTCTTTTCGTACTATGGAAAAGGAGGAGGGTTCTTCTAGGGTACCCTTCGTGGAACTGAGCCTCTTCTCTCCTAGTCGAGATGCATCACCCCCAGATTCGCCTCCGCTCCTTGGCTGGCCTATGCGTCCCCATCCATCGTCTCAGTCACCGGTCGTTCAAGTTCATCGAACCGCAGCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

45.65

Weight (kDa)

9.25

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57680 AT1G57680 AT1G57680
fragaria_vesca FvH4_5g28140 FvH4_5g28140
malus_domestica MD08G1245000.v1.1 MD15G1440100.v1.1
prunus_persica Prupe.1G582400_v2.0.a1
pyrus_communis pycom08g21250 pycom15g38800
rosa_chinensis RchiOBHm_Chr7g0220291
rosa_laevigata RLG00000002242
rosa_multiflora Rmu_sc0001136.1_g000030
rosa_roxburghii Rroxscaffold_3G00239640
rosa_rugosa Rorug07G0191900.1
rosa_samantha Rh7AG333900 Rh7BG323200 Rh7CG351600 Rh7DG330200
rosa_wichuraiana Rw7G028170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 385
AatII GACGTC 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 1078
AccB1I GGYRCC 1 cut(s) 1078
AccBSI CCGCTC 1 cut(s) 1144
AciI CCGC 5 cut(s) 326, 655, 875, 1142, 1209
AclWI GGATC 1 cut(s) 1039
AcsI RAATTY 1 cut(s) 393
AcuI CTGAAG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 241
AdeI CACNNNGTG 1 cut(s) 35
AfaI GTAC 3 cut(s) 544, 1048, 1080
AfiI CCNNNNNNNGG 3 cut(s) 454, 874, 1148
AgeI ACCGGT 1 cut(s) 1186
AgsI TTSAA 5 cut(s) 259, 274, 723, 794, 1196
AhdI GACNNNNNGTC 1 cut(s) 48
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 5 cut(s) 142, 425, 469, 797, 1214
AluI AGCT 5 cut(s) 142, 425, 469, 797, 1214
Alw21I GWGCWC 2 cut(s) 596, 783
Alw26I GTCTC 5 cut(s) 83, 232, 313, 999, 1182
AlwI GGATC 1 cut(s) 1039
AoxI GGCC 2 cut(s) 157, 1154
ApeKI GCWGC 2 cut(s) 20, 1211
ApoI RAATTY 1 cut(s) 393
AsiGI ACCGGT 1 cut(s) 1186
Asp718I GGTACC 1 cut(s) 1078
AspLEI GCGC 1 cut(s) 370
AspS9I GGNCC 2 cut(s) 158, 488
AsuC2I CCSGG 1 cut(s) 838
AsuHPI GGTGA 3 cut(s) 224, 1116, 1176
AvaII GGWCC 1 cut(s) 488
BanI GGYRCC 1 cut(s) 1078
Bbv12I GWGCWC 2 cut(s) 596, 783
BbvI GCAGC 1 cut(s) 7
BccI CCATC 4 cut(s) 605, 626, 1176, 1180
BclI TGATCA 2 cut(s) 559, 931
BcnI CCSGG 1 cut(s) 838
BcoDI GTCTC 5 cut(s) 83, 232, 313, 999, 1182
BfaI CTAG 4 cut(s) 53, 1074, 1110, 1215
BfmI CTRYAG 1 cut(s) 686
BisI GCNGC 2 cut(s) 21, 1212
BlpI GCTNAGC 1 cut(s) 590
BlsI GCNGC 2 cut(s) 22, 1213
Bme1390I CCNGG 1 cut(s) 838
Bme18I GGWCC 1 cut(s) 488
BmeRI GACNNNNNGTC 1 cut(s) 48
BmgT120I GGNCC 2 cut(s) 158, 488
BmiI GGNNCC 3 cut(s) 330, 490, 1080
BmrFI CCNGG 1 cut(s) 838
BmrI ACTGGG 1 cut(s) 863
BmsI GCATC 2 cut(s) 1108, 1130
BmuI ACTGGG 1 cut(s) 863
Bpu1102I GCTNAGC 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 838
BsaHI GRCGYC 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 5 cut(s) 161, 316, 837, 889, 1147
BsaWI WCCGGW 1 cut(s) 1186
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 874, 1148
Bse118I RCCGGY 1 cut(s) 1186
Bse1I ACTGG 1 cut(s) 869
Bse3DI GCAATG 1 cut(s) 954
BseDI CCNNGG 5 cut(s) 161, 316, 837, 889, 1147
BseGI GGATG 1 cut(s) 1168
BseLI CCNNNNNNNGG 3 cut(s) 454, 874, 1148
BseMI GCAATG 1 cut(s) 954
BseMII CTCAG 4 cut(s) 134, 581, 1085, 1193
BseNI ACTGG 1 cut(s) 869
BseRI GAGGAG 1 cut(s) 1076
BseXI GCAGC 1 cut(s) 7
BsgI GTGCAG 1 cut(s) 559
Bsh1285I CGRYCG 1 cut(s) 1191
BshFI GGCC 2 cut(s) 159, 1156
BshNI GGYRCC 1 cut(s) 1078
BshTI ACCGGT 1 cut(s) 1186
BsiEI CGRYCG 1 cut(s) 1191
BsiHKAI GWGCWC 2 cut(s) 596, 783
BsiSI CCGG 2 cut(s) 837, 1187
BslFI GGGAC 1 cut(s) 1149
BslI CCNNNNNNNGG 3 cut(s) 454, 874, 1148
BsmAI GTCTC 5 cut(s) 83, 232, 313, 999, 1182
BsmBI CGTCTC 2 cut(s) 232, 1182
BsmFI GGGAC 1 cut(s) 1149
BsmI GAATGC 1 cut(s) 117
BsnI GGCC 2 cut(s) 159, 1156
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 596, 783
Bsp143I GATC 3 cut(s) 559, 931, 1031
Bsp1720I GCTNAGC 1 cut(s) 590
BspACI CCGC 5 cut(s) 326, 655, 875, 1142, 1209
BspANI GGCC 2 cut(s) 159, 1156
BspCNI CTCAG 4 cut(s) 135, 582, 1086, 1192
BspLI GGNNCC 3 cut(s) 330, 490, 1080
BspMAI CTGCAG 1 cut(s) 690
BspPI GGATC 1 cut(s) 1039
BspQI GCTCTTC 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 1078
BspTNI GGTCTC 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 1144
BsrDI GCAATG 1 cut(s) 954
BsrFI RCCGGY 1 cut(s) 1186
BsrI ACTGG 1 cut(s) 869
BssAI RCCGGY 1 cut(s) 1186
BssECI CCNNGG 5 cut(s) 161, 316, 837, 889, 1147
BssMI GATC 3 cut(s) 559, 931, 1031
BssNI GRCGYC 1 cut(s) 241
BssT1I CCWWGG 4 cut(s) 161, 316, 889, 1147
Bst4CI ACNGT 2 cut(s) 512, 547
Bst6I CTCTTC 4 cut(s) 86, 468, 686, 1106
BstACI GRCGYC 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 854, 1154
BstDEI CTNAG 5 cut(s) 143, 398, 590, 1094, 1179
BstF5I GGATG 1 cut(s) 1168
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 3 cut(s) 562, 934, 1034
BstMAI GTCTC 5 cut(s) 83, 232, 313, 999, 1182
BstMBI GATC 3 cut(s) 559, 931, 1031
BstMCI CGRYCG 1 cut(s) 1191
BstMWI GCNNNNNNNGC 2 cut(s) 652, 818
BstNSI RCATGY 1 cut(s) 856
BstSCI CCNGG 1 cut(s) 836
BstSFI CTRYAG 1 cut(s) 686
BstV1I GCAGC 1 cut(s) 7
BsuRI GGCC 2 cut(s) 159, 1156
BtsCI GGATG 1 cut(s) 1168
BtsIMutI CAGTG 2 cut(s) 160, 552
Cac8I GCNNGC 2 cut(s) 854, 1154
CfoI GCGC 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 1186
Cfr13I GGNCC 2 cut(s) 158, 488
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 3 cut(s) 543, 1047, 1079
CspAI ACCGGT 1 cut(s) 1186
CviAII CATG 3 cut(s) 333, 563, 853
CviQI GTAC 3 cut(s) 543, 1047, 1079
DdeI CTNAG 5 cut(s) 143, 398, 590, 1094, 1179
DpnI GATC 3 cut(s) 561, 933, 1033
DpnII GATC 3 cut(s) 559, 931, 1031
DraI TTTAAA 1 cut(s) 663
DraIII CACNNNGTG 1 cut(s) 35
DrdI GACNNNNNNGTC 1 cut(s) 385
DriI GACNNNNNGTC 1 cut(s) 48
DseDI GACNNNNNNGTC 1 cut(s) 385
Eam1104I CTCTTC 4 cut(s) 86, 468, 686, 1106
Eam1105I GACNNNNNGTC 1 cut(s) 48
EarI CTCTTC 4 cut(s) 86, 468, 686, 1106
EciI GGCGGA 1 cut(s) 670
Eco130I CCWWGG 4 cut(s) 161, 316, 889, 1147
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 488
Eco57I CTGAAG 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 393
EcoT14I CCWWGG 4 cut(s) 161, 316, 889, 1147
EcoT22I ATGCAT 2 cut(s) 854, 1123
ErhI CCWWGG 4 cut(s) 161, 316, 889, 1147
Esp3I CGTCTC 2 cut(s) 232, 1182
FaeI CATG 3 cut(s) 336, 566, 856
FalI AAGNNNNNCTT 2 cut(s) 730, 762
FaqI GGGAC 1 cut(s) 1149
FatI CATG 3 cut(s) 332, 562, 852
FauNDI CATATG 2 cut(s) 47, 972
FbaI TGATCA 2 cut(s) 559, 931
Fnu4HI GCNGC 2 cut(s) 21, 1212
FokI GGATG 1 cut(s) 1155
Fsp4HI GCNGC 2 cut(s) 21, 1212
FspBI CTAG 4 cut(s) 53, 1074, 1110, 1215
GlaI GCGC 1 cut(s) 369
GluI GCNGC 2 cut(s) 21, 1212
HaeIII GGCC 2 cut(s) 159, 1156
HapII CCGG 2 cut(s) 837, 1187
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 1 cut(s) 370
Hin1I GRCGYC 1 cut(s) 241
Hin1II CATG 3 cut(s) 336, 566, 856
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HinfI GANTC 8 cut(s) 174, 286, 386, 878, 967, 990, 1007, 1133
HpaII CCGG 2 cut(s) 837, 1187
HphI GGTGA 3 cut(s) 224, 1116, 1176
Hpy166II GTNNAC 5 cut(s) 42, 515, 550, 862, 899
Hpy188I TCNGA 2 cut(s) 173, 936
Hpy188III TCNNGA 5 cut(s) 64, 343, 390, 987, 1115
Hpy8I GTNNAC 5 cut(s) 42, 515, 550, 862, 899
HpyAV CCTTC 5 cut(s) 80, 580, 645, 740, 1093
HpyCH4III ACNGT 2 cut(s) 512, 547
HpyCH4IV ACGT 2 cut(s) 241, 783
HpyCH4V TGCA 9 cut(s) 87, 194, 294, 376, 576, 688, 821, 852, 1121
HpyF10VI GCNNNNNNNGC 2 cut(s) 652, 818
HpyF3I CTNAG 5 cut(s) 143, 398, 590, 1094, 1179
HpySE526I ACGT 2 cut(s) 241, 783
Hsp92I GRCGYC 1 cut(s) 241
Hsp92II CATG 3 cut(s) 336, 566, 856
HspAI GCGC 1 cut(s) 368
KpnI GGTACC 1 cut(s) 1082
Ksp22I TGATCA 2 cut(s) 559, 931
Kzo9I GATC 3 cut(s) 559, 931, 1031
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 1 cut(s) 1149
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 2 cut(s) 1108, 1130
MaeI CTAG 4 cut(s) 53, 1074, 1110, 1215
MaeII ACGT 2 cut(s) 241, 783
MaeIII GTNAC 4 cut(s) 299, 428, 742, 1182
MalI GATC 3 cut(s) 561, 933, 1033
MbiI CCGCTC 1 cut(s) 1144
MboI GATC 3 cut(s) 559, 931, 1031
MboII GAAGA 5 cut(s) 73, 455, 703, 1062, 1093
MhlI GDGCHC 2 cut(s) 596, 783
MluCI AATT 6 cut(s) 15, 393, 483, 521, 665, 1035
MlyI GAGTC 3 cut(s) 395, 976, 1001
MnlI CCTC 7 cut(s) 71, 458, 970, 1054, 1057, 1109, 1149
Mph1103I ATGCAT 2 cut(s) 854, 1123
MseI TTAA 6 cut(s) 227, 267, 482, 662, 737, 924
MslI CAYNNNNRTG 2 cut(s) 599, 826
MspA1I CMGCKG 2 cut(s) 142, 425
MspI CCGG 2 cut(s) 837, 1187
MspR9I CCNGG 1 cut(s) 838
Mva1269I GAATGC 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 652, 818
NciI CCSGG 1 cut(s) 838
NdeI CATATG 2 cut(s) 47, 972
NdeII GATC 3 cut(s) 559, 931, 1031
NlaIII CATG 3 cut(s) 336, 566, 856
NlaIV GGNNCC 3 cut(s) 330, 490, 1080
NmuCI GTSAC 1 cut(s) 1182
NsiI ATGCAT 2 cut(s) 854, 1123
NspI RCATGY 1 cut(s) 856
OliI CACNNNNGTG 1 cut(s) 599
PaeI GCATGC 1 cut(s) 856
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 1 cut(s) 117
PfeI GAWTC 5 cut(s) 174, 286, 878, 990, 1133
PinAI ACCGGT 1 cut(s) 1186
PkrI GCNGC 2 cut(s) 22, 1213
PleI GAGTC 3 cut(s) 394, 975, 1001
PpsI GAGTC 3 cut(s) 394, 975, 1001
PspN4I GGNNCC 3 cut(s) 330, 490, 1080
PspPI GGNCC 2 cut(s) 158, 488
PstI CTGCAG 1 cut(s) 690
PvuII CAGCTG 2 cut(s) 142, 425
RsaI GTAC 3 cut(s) 544, 1048, 1080
RsaNI GTAC 3 cut(s) 543, 1047, 1079
RseI CAYNNNNRTG 2 cut(s) 599, 826
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 6 cut(s) 227, 267, 482, 662, 737, 924
SatI GCNGC 2 cut(s) 21, 1212
Sau3AI GATC 3 cut(s) 559, 931, 1031
Sau96I GGNCC 2 cut(s) 158, 488
SchI GAGTC 3 cut(s) 395, 976, 1001
ScrFI CCNGG 1 cut(s) 838
SduI GDGCHC 2 cut(s) 596, 783
SfaNI GCATC 2 cut(s) 1108, 1130
SfcI CTRYAG 1 cut(s) 686
SinI GGWCC 1 cut(s) 488
SmiMI CAYNNNNRTG 2 cut(s) 599, 826
SphI GCATGC 1 cut(s) 856
Sse9I AATT 6 cut(s) 15, 393, 483, 521, 665, 1035
SsiI CCGC 5 cut(s) 326, 655, 875, 1142, 1209
SspMI CTAG 4 cut(s) 53, 1074, 1110, 1215
StyD4I CCNGG 1 cut(s) 836
StyI CCWWGG 4 cut(s) 161, 316, 889, 1147
TaaI ACNGT 2 cut(s) 512, 547
TaiI ACGT 2 cut(s) 244, 786
TaqI TCGA 2 cut(s) 1114, 1204
TasI AATT 6 cut(s) 15, 393, 483, 521, 665, 1035
TatI WGTACW 1 cut(s) 542
TfiI GAWTC 5 cut(s) 174, 286, 878, 990, 1133
Tru1I TTAA 6 cut(s) 227, 267, 482, 662, 737, 924
Tru9I TTAA 6 cut(s) 227, 267, 482, 662, 737, 924
TscAI CASTG 2 cut(s) 160, 552
TseFI GTSAC 1 cut(s) 1182
TseI GCWGC 2 cut(s) 20, 1211
Tsp45I GTSAC 1 cut(s) 1182
TspDTI ATGAA 2 cut(s) 172, 1190
TspGWI ACGGA 2 cut(s) 212, 723
TspRI CASTG 2 cut(s) 160, 552
VpaK11BI GGWCC 1 cut(s) 488
XapI RAATTY 1 cut(s) 393
XceI RCATGY 1 cut(s) 856
XspI CTAG 4 cut(s) 53, 1074, 1110, 1215
ZraI GACGTC 1 cut(s) 242
Zsp2I ATGCAT 2 cut(s) 854, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.