Rmu_sc0001136.1_g000030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001136.1
OG0010894
Physical Location & Seq
Reverse (-)
143328 .. 145535
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001136.1_g000030.1.cds

Sequence Viewer

Length: 1209 bp
atgctcggacttcgccagatcacgattctcggctttgtggctggcgtttacgctacccgaggatcttttgtatccatgctggcagagagcatgcgtggaatctgtgcttcgggtaatgccccagacgagaattgctgccgatacactcgtcagggttttgttcaactcagttatttcagtggcccttggataatccgaataactttcattttgtttgcaatctggtggggaattggtgaaattatccggttaagtttgttgagacgggaaggaagactgttgaatacccttaactggaaatggcaggagactgtctgcaaatgctatattgtctcaaacctggggtttgcagaaccttgcctattcctgacccttgtgttcctacttcgtgctcctttgcatacaatggagtcgggtattctaagccgacaatggaatgggaaaacagctggttacattcttttctactgcctcccaatggttcttcttcagcttgttgtcatagtaattggacccacgctgcacaataataaaaagtttccttcctactttaccagtacaatgaacaagactgatcagatggccctctgcacttaccctttactgagtacgttacttcttgggatttttgccatcattctaactgcctacgtgttttggcttggaaggcagattttaaagatggtcatcaataagggtctgcagaaaagagtttatacattgatattctcagtttcaagtttccttccattgagggttactttccttggtttctctgttttatctaaaccagagcactttcagtttgaagctcttgctttcttggcttttcttgcccttttatgttgtgcgggtgtgtgtatatgcatgcttgtttactgcccggttgcggattctttagcgctggggagtttacaggacttggagggtaggaggagacttgatgatggtcaaaatgacaccatttctcttattgctaaccagagtcatatggaagaaagtgctggatttagtcccagtagaagctctgatgtgtcaacaaagcagcgtggatcaatttctttccgtgccttgggaaaggaggatgggtcttctaggggaccatttgtggaattgagcctcttctctcctagtgaagatgcaacacccccagaatcacctccgcttcttggctggcctatgcgtccctctccttcacctcaccagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

44.84

Weight (kDa)

8.95

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57680 AT1G57680 AT1G57680
fragaria_vesca FvH4_5g28140 FvH4_5g28140
malus_domestica MD08G1245000.v1.1 MD15G1440100.v1.1
prunus_persica Prupe.1G582400_v2.0.a1
pyrus_communis pycom08g21250 pycom15g38800
rosa_chinensis RchiOBHm_Chr7g0220291
rosa_laevigata RLG00000002242
rosa_multiflora Rmu_sc0001136.1_g000030
rosa_roxburghii Rroxscaffold_3G00239640
rosa_rugosa Rorug07G0191900.1
rosa_samantha Rh7AG333900 Rh7BG323200 Rh7CG351600 Rh7DG330200
rosa_wichuraiana Rw7G028170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 851, 890, 1163
AclWI GGATC 2 cut(s) 70, 1060
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 2 cut(s) 559, 610
AfeI AGCGCT 1 cut(s) 903
AfiI CCNNNNNNNGG 5 cut(s) 294, 478, 889, 1072, 1169
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 4 cut(s) 164, 283, 738, 809
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 4 cut(s) 449, 493, 812, 1026
AluI AGCT 4 cut(s) 449, 493, 812, 1026
Alw21I GWGCWC 2 cut(s) 394, 798
Alw26I GTCTC 4 cut(s) 256, 302, 337, 931
AlwI GGATC 2 cut(s) 70, 1060
Ama87I CYCGRG 1 cut(s) 57
Aor51HI AGCGCT 1 cut(s) 903
AoxI GGCC 3 cut(s) 181, 582, 1175
ApeKI GCWGC 3 cut(s) 135, 520, 1045
AspLEI GCGC 1 cut(s) 904
AspS9I GGNCC 4 cut(s) 182, 512, 583, 1100
AsuC2I CCSGG 1 cut(s) 884
AsuHPI GGTGA 4 cut(s) 248, 1149, 1188, 1193
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 2 cut(s) 512, 1100
BaeI ACNNNNGTAYC 2 cut(s) 133, 166
BbsI GAAGAC 2 cut(s) 280, 1083
Bbv12I GWGCWC 2 cut(s) 394, 798
BbvI GCAGC 3 cut(s) 122, 507, 1057
BccI CCATC 5 cut(s) 574, 641, 676, 941, 1079
BciT130I CCWGG 1 cut(s) 341
BciVI GTATCC 1 cut(s) 82
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 884
BcoDI GTCTC 4 cut(s) 256, 302, 337, 931
BfaI CTAG 2 cut(s) 1095, 1131
BfmI CTRYAG 1 cut(s) 701
BfoI RGCGCY 1 cut(s) 905
BfuI GTATCC 1 cut(s) 82
BisI GCNGC 3 cut(s) 136, 521, 1046
BlsI GCNGC 3 cut(s) 137, 522, 1047
Bme1390I CCNGG 2 cut(s) 341, 884
Bme18I GGWCC 2 cut(s) 512, 1100
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 4 cut(s) 182, 512, 583, 1100
BmiI GGNNCC 2 cut(s) 514, 1101
BmrFI CCNGG 2 cut(s) 341, 884
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 1 cut(s) 1129
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 2 cut(s) 280, 1083
BpuMI CCSGG 1 cut(s) 884
BsaAI YACGTR 1 cut(s) 652
BsaBI GATNNNNATC 1 cut(s) 686
BsaJI CCNNGG 5 cut(s) 58, 185, 340, 766, 1071
BsaWI WCCGGW 1 cut(s) 246
BsaXI ACNNNNNCTCC 2 cut(s) 1073, 1103
Bsc4I CCNNNNNNNGG 5 cut(s) 294, 478, 889, 1072, 1169
Bse1I ACTGG 4 cut(s) 299, 555, 1017, 1204
Bse8I GATNNNNATC 1 cut(s) 686
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 5 cut(s) 58, 185, 340, 766, 1071
BseGI GGATG 1 cut(s) 1090
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 5 cut(s) 294, 478, 889, 1072, 1169
BseMII CTCAG 3 cut(s) 181, 596, 744
BseNI ACTGG 4 cut(s) 299, 555, 1017, 1204
BseRI GAGGAG 1 cut(s) 949
BseXI GCAGC 3 cut(s) 122, 507, 1057
BseYI CCCAGC 1 cut(s) 904
BsgI GTGCAG 2 cut(s) 506, 574
BshFI GGCC 3 cut(s) 183, 584, 1177
BsiHKAI GWGCWC 2 cut(s) 394, 798
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 2 cut(s) 247, 884
BslFI GGGAC 3 cut(s) 999, 1113, 1170
BslI CCNNNNNNNGG 5 cut(s) 294, 478, 889, 1072, 1169
BsmAI GTCTC 4 cut(s) 256, 302, 337, 931
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 3 cut(s) 999, 1113, 1170
BsnI GGCC 3 cut(s) 183, 584, 1177
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 394, 798
Bsp143I GATC 4 cut(s) 18, 62, 574, 1052
BspACI CCGC 3 cut(s) 851, 890, 1163
BspANI GGCC 3 cut(s) 183, 584, 1177
BspCNI CTCAG 3 cut(s) 180, 597, 743
BspLI GGNNCC 2 cut(s) 514, 1101
BspMAI CTGCAG 1 cut(s) 705
BspPI GGATC 2 cut(s) 70, 1060
BsrI ACTGG 4 cut(s) 299, 555, 1017, 1204
BssECI CCNNGG 5 cut(s) 58, 185, 340, 766, 1071
BssMI GATC 4 cut(s) 18, 62, 574, 1052
BssT1I CCWWGG 3 cut(s) 185, 766, 1071
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 2 cut(s) 279, 313
Bst6I CTCTTC 1 cut(s) 1127
BstBAI YACGTR 1 cut(s) 652
BstC8I GCNNGC 5 cut(s) 43, 81, 92, 869, 1175
BstDEI CTNAG 4 cut(s) 167, 422, 605, 730
BstF5I GGATG 1 cut(s) 1090
BstH2I RGCGCY 1 cut(s) 905
BstHHI GCGC 1 cut(s) 904
BstKTI GATC 4 cut(s) 21, 65, 577, 1055
BstMAI GTCTC 4 cut(s) 256, 302, 337, 931
BstMBI GATC 4 cut(s) 18, 62, 574, 1052
BstMWI GCNNNNNNNGC 3 cut(s) 667, 824, 833
BstNI CCWGG 1 cut(s) 341
BstNSI RCATGY 2 cut(s) 94, 871
BstSCI CCNGG 2 cut(s) 339, 882
BstSFI CTRYAG 1 cut(s) 701
BstV1I GCAGC 3 cut(s) 122, 507, 1057
BstV2I GAAGAC 2 cut(s) 280, 1083
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuI GTATCC 1 cut(s) 82
BsuRI GGCC 3 cut(s) 183, 584, 1177
BtsCI GGATG 1 cut(s) 1090
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 5 cut(s) 43, 81, 92, 869, 1175
CfoI GCGC 1 cut(s) 904
Cfr13I GGNCC 4 cut(s) 182, 512, 583, 1100
CseI GACGC 1 cut(s) 1172
Csp6I GTAC 2 cut(s) 558, 609
CviAII CATG 3 cut(s) 76, 91, 868
CviQI GTAC 2 cut(s) 558, 609
DdeI CTNAG 4 cut(s) 167, 422, 605, 730
DpnI GATC 4 cut(s) 20, 64, 576, 1054
DpnII GATC 4 cut(s) 18, 62, 574, 1052
DraI TTTAAA 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 1127
EarI CTCTTC 1 cut(s) 1127
Eco130I CCWWGG 3 cut(s) 185, 766, 1071
Eco47I GGWCC 2 cut(s) 512, 1100
Eco47III AGCGCT 1 cut(s) 903
Eco57I CTGAAG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 339
EcoT14I CCWWGG 3 cut(s) 185, 766, 1071
EcoT22I ATGCAT 1 cut(s) 869
ErhI CCWWGG 3 cut(s) 185, 766, 1071
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 3 cut(s) 79, 94, 871
FaqI GGGAC 3 cut(s) 999, 1113, 1170
FatI CATG 3 cut(s) 75, 90, 867
FauI CCCGC 1 cut(s) 844
FauNDI CATATG 1 cut(s) 990
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 3 cut(s) 136, 521, 1046
FokI GGATG 1 cut(s) 1097
Fsp4HI GCNGC 3 cut(s) 136, 521, 1046
FspBI CTAG 2 cut(s) 1095, 1131
GlaI GCGC 1 cut(s) 903
GluI GCNGC 3 cut(s) 136, 521, 1046
GsaI CCCAGC 1 cut(s) 908
HaeII RGCGCY 1 cut(s) 905
HaeIII GGCC 3 cut(s) 183, 584, 1177
HapII CCGG 2 cut(s) 247, 884
HgaI GACGC 1 cut(s) 1172
HhaI GCGC 1 cut(s) 904
Hin1II CATG 3 cut(s) 79, 94, 871
Hin6I GCGC 1 cut(s) 902
HinP1I GCGC 1 cut(s) 902
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HinfI GANTC 6 cut(s) 25, 99, 410, 893, 985, 1154
HpaII CCGG 2 cut(s) 247, 884
HphI GGTGA 4 cut(s) 248, 1149, 1188, 1193
Hpy166II GTNNAC 4 cut(s) 49, 877, 914, 1038
Hpy188I TCNGA 4 cut(s) 8, 197, 579, 1030
Hpy188III TCNNGA 2 cut(s) 22, 367
Hpy8I GTNNAC 4 cut(s) 49, 877, 914, 1038
HpyAV CCTTC 5 cut(s) 263, 552, 660, 755, 1203
HpyCH4III ACNGT 2 cut(s) 279, 313
HpyCH4IV ACGT 2 cut(s) 611, 651
HpyCH4V TGCA 9 cut(s) 218, 318, 350, 400, 523, 591, 703, 867, 1142
HpyF10VI GCNNNNNNNGC 3 cut(s) 667, 824, 833
HpyF3I CTNAG 4 cut(s) 167, 422, 605, 730
HpySE526I ACGT 2 cut(s) 611, 651
Hsp92II CATG 3 cut(s) 79, 94, 871
HspAI GCGC 1 cut(s) 902
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 4 cut(s) 18, 62, 574, 1052
LmnI GCTCC 1 cut(s) 397
Lsp1109I GCAGC 3 cut(s) 122, 507, 1057
LweI GCATC 1 cut(s) 1129
MaeI CTAG 2 cut(s) 1095, 1131
MaeII ACGT 2 cut(s) 611, 651
MaeIII GTNAC 3 cut(s) 452, 612, 757
MalI GATC 4 cut(s) 20, 64, 576, 1054
MboI GATC 4 cut(s) 18, 62, 574, 1052
MboII GAAGA 7 cut(s) 285, 476, 479, 1007, 1083, 1114, 1148
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 2 cut(s) 394, 798
MluCI AATT 6 cut(s) 130, 231, 240, 507, 1056, 1112
MlyI GAGTC 2 cut(s) 419, 994
Mph1103I ATGCAT 1 cut(s) 869
MseI TTAA 3 cut(s) 251, 291, 677
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 2 cut(s) 247, 884
MspR9I CCNGG 2 cut(s) 341, 884
MvaI CCWGG 1 cut(s) 341
MwoI GCNNNNNNNGC 3 cut(s) 667, 824, 833
NciI CCSGG 1 cut(s) 884
NdeI CATATG 1 cut(s) 990
NdeII GATC 4 cut(s) 18, 62, 574, 1052
NlaIII CATG 3 cut(s) 79, 94, 871
NlaIV GGNNCC 2 cut(s) 514, 1101
NmeAIII GCCGAG 1 cut(s) 9
NsiI ATGCAT 1 cut(s) 869
NspI RCATGY 2 cut(s) 94, 871
PaeI GCATGC 2 cut(s) 94, 871
PfeI GAWTC 4 cut(s) 25, 99, 893, 1154
PkrI GCNGC 3 cut(s) 137, 522, 1047
PleI GAGTC 2 cut(s) 418, 993
PpsI GAGTC 2 cut(s) 418, 993
Ppu21I YACGTR 1 cut(s) 652
Psp6I CCWGG 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 904
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 514, 1101
PspPI GGNCC 4 cut(s) 182, 512, 583, 1100
PstI CTGCAG 1 cut(s) 705
PsuI RGATCY 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 2 cut(s) 559, 610
RsaNI GTAC 2 cut(s) 558, 609
SaqAI TTAA 3 cut(s) 251, 291, 677
SatI GCNGC 3 cut(s) 136, 521, 1046
Sau3AI GATC 4 cut(s) 18, 62, 574, 1052
Sau96I GGNCC 4 cut(s) 182, 512, 583, 1100
SchI GAGTC 2 cut(s) 419, 994
ScrFI CCNGG 2 cut(s) 341, 884
SduI GDGCHC 2 cut(s) 394, 798
SfaNI GCATC 1 cut(s) 1129
SfcI CTRYAG 1 cut(s) 701
SinI GGWCC 2 cut(s) 512, 1100
SphI GCATGC 2 cut(s) 94, 871
Sse9I AATT 6 cut(s) 130, 231, 240, 507, 1056, 1112
SsiI CCGC 3 cut(s) 851, 890, 1163
SspMI CTAG 2 cut(s) 1095, 1131
StyD4I CCNGG 2 cut(s) 339, 882
StyI CCWWGG 3 cut(s) 185, 766, 1071
TaaI ACNGT 2 cut(s) 279, 313
TaiI ACGT 2 cut(s) 614, 654
TasI AATT 6 cut(s) 130, 231, 240, 507, 1056, 1112
TatI WGTACW 1 cut(s) 557
TfiI GAWTC 4 cut(s) 25, 99, 893, 1154
Tru1I TTAA 3 cut(s) 251, 291, 677
Tru9I TTAA 3 cut(s) 251, 291, 677
TscAI CASTG 1 cut(s) 184
TseI GCWGC 3 cut(s) 135, 520, 1045
TspDTI ATGAA 2 cut(s) 196, 578
TspGWI ACGGA 1 cut(s) 1055
TspRI CASTG 1 cut(s) 184
VpaK11BI GGWCC 2 cut(s) 512, 1100
XceI RCATGY 2 cut(s) 94, 871
XspI CTAG 2 cut(s) 1095, 1131
Zsp2I ATGCAT 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.