MD09G1011500.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012914
Physical Location & Seq
Reverse (-)
756530 .. 760747
4218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1011500.v1.1.491

Sequence Viewer

Length: 2028 bp
ATGGCGGGAAAGGAGGAAGTGAAGGGGCGGCGAGGAGCGACCGGCGCCGGCGGCGAAGAGGTCTGCTGGAGAAAGAAAGTGGACGACAACCTAAACAGGCTCCATTCTCTTTCCTTCGGAGCCGAGCTCGCCCTCGAGAGGCACGACTTCTCCTCCGCTCAGATTCTCGGCCTTCGTCTCCTTGGCTTCCTCGACTCCCAATCTCAGTCTTCGCTCGACCACGCCTTCATCCGCCCCATTCGTCGAGAAACCGAAGCCAAACTCCACGCCGCCCGCCGCGAGCTCATTCCTCTTTCCGATCGCAAAGTCTTTGAGCAAGCAAAGAAGGCGCCGGGCTGTGTTTTTGGCACAAATGGAGATATTGACATTGAGAAGATTAAGCAGTCAAAGTACTTTCAGGCTCTTCTTCAGCAATCTAGTAGCGGAAGGGCTGTGAATGATTTGGGAGATCAATTGGAGAGGCAGGACAAGTTGACTAGCAAGTTTTCAAAACCTATGACACAAACAAAGTTGACATCGTTGTATAAACAGAACTCGATGGTGGAAAGTAATGGAAAATCCAAGGGTTCTTTGGCCTCTAGAAGTAACGGCTCTGAGGACTGCGTTGGTGTAGAAAAGCTTCACACTCTCCACAACCATAATGGTGGTCATAGTGCCTCTTCTTTTCAAAAAGTTGAAGAGGAAGGAGCTTATGGGAATACCTTTAGGGCTAAACGTGCACACAAGGAATTTACTAGCCCCATCATTGATATAGCTAAGTCCCCATCAGCCAACGAAGATGCTAAAGCTGATGGTTCTGGAAATAGCTTTGTTACTGCCAAAGCCAAATTGGAAATGGATGTGAGGCAAAGAAGAGGGTTAGCGCCAGCAAGATCACCAAATTCTTGTGTCTCTCCTCAAAGTGATAACAATGCTACTAATAGGGGTTATGGTATGAGATCATATGGTTATTCACATCGGGGAGCCCGTGGTAATTTTGTCCCCCCTATTAAATCTAATGAGGGAAACGTTGGGAATGTGACTTCACGAGTCGCTGGAAAGTCAGACGATGCATTAGGTGACTCAACTAAGAGATGTTTGGAACTGCTCTGTGGTCCTGATGGAGAGCTTCCAGAAAAACTAAGGAACTTGGAACCTCGTCTTCTTGAGCATGTTAGTAATGAGATTATGGACAAGGATCCTAATGTTCGATGGAATGATATAGCTGGTTTGGAGCATGCTAAAAAATGTGTAACCGAGATGGTCATATGGCCTCTACTACGTCCTGACATTTTTAAGGGCTGTCGTTCTCCTGGGAAAGGCCTTCTTCTCTTTGGTCCACCAGGAACAGGAAAAACAATGATTGGGAAAGCCATAGCTGGAGAGGCAAAAGCAACCTTTTTCTACATATCTGCCAGTTCATTAACAAGCAAATGGATTGGCGAGGGTGAAAAGCTAGTGCGAGCACTGTTTGGAGTCGCAAGTTGTCGCCAGCCAGCTGTTATTTTTGTTGATGAAATTGATTCACTTCTCTCACAGCGTAAGTCAGAAGGTGAGCACGAATCAAGTAGACGACTCAAAACGCAGTTCCTTATTGAGATGGAAGGCTTTGACAGTGGGAGTGAGCAAATTCTTCTTATAGGAGCAACGAACCGACCCCAAGAACTTGATGAAGCAGCACGGAGGCGACTTACCAAGAGGCTTTACATTCCCCTCCCTTCATTAGCGAGAGCCTGGATCATACGGAATCTCTTAGAAAAGGATGGACTGTTCAAACTTTCAAGGGAGGACATTGATAGCATATGCAATTTAACCCAAGGTTACTCAGGATCGGACATGAAAAACTTGGTGAAGGATGCCTCAATGGGTCCACTGAGGGAAGCTCTGAAACAAGGTATCGAAATAACAAAGCTCAAAAAAGAGGATATGCGTCCAGTAACTGTTCAGGACTTTGAGAGTGCATTGCAAGAAGTGAGGCCTTCTGTTTCCTTGAACGAGCTCGGTACGTACGAGGAATGGAACAAGCAATTTGGAAGCTTGTCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000228 GO:0000280 GO:0000287 GO:0001503 GO:0001649 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006163 GO:0006282 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007140 GO:0007276 GO:0008150 GO:0008152 GO:0008283 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009314 GO:0009628 GO:0009892 GO:0009987 GO:0010212 GO:0010520 GO:0010564 GO:0010569 GO:0010605 GO:0010639 GO:0010941 GO:0010948 GO:0016043 GO:0016787 GO:0017144 GO:0019219 GO:0019222 GO:0019637 GO:0019693 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0033687 GO:0034641 GO:0040020 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045128 GO:0045786 GO:0045835 GO:0045910 GO:0045934 GO:0046034 GO:0046483 GO:0046872 GO:0048232 GO:0048285 GO:0048471 GO:0048519 GO:0048523 GO:0048583 GO:0048609 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051321 GO:0051445 GO:0051447 GO:0051704 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0055086 GO:0060255 GO:0060548 GO:0060631 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0071840 GO:0072521 GO:0080090 GO:0080134 GO:0080135 GO:0104004 GO:0110029 GO:0140013 GO:1901135 GO:1901360 GO:1901564 GO:1903046 GO:2000241 GO:2000242 GO:2000779 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Vps4_C

Protein Analysis

676

Amino Acids

74.48

Weight (kDa)

9.02

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FIGL1_N PF24347 20 - 94 1.6e-36 FIGL1 N-terminal domain
AAA PF00004 435 - 565 2.5e-39 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 589 - 630 6.1e-09 AAA+ lid domain
Vps4_C PF09336 637 - 671 1.7e-08 Vps4 C terminal oligomerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012914)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27120
fragaria_vesca FvH4_6g53320
malus_domestica MD09G1011500.v1.1 MD17G1012000.v1.1
prunus_persica Prupe.3G305500_v2.0.a1
pyrus_communis pycom111g00980 pycom17g00970
rosa_chinensis RchiOBHm_Chr2g0175481
rosa_laevigata RLG00000022331
rosa_multiflora Rmu_sc0003302.1_g000002 Rmu_sc0021955.1_g000001
rosa_roxburghii Rroxscaffold_2G00077280
rosa_rugosa Rorug02G0585100
rosa_samantha Rh2AG664600 Rh2BG675300 Rh2CG638500 Rh2DG689300
rosa_wichuraiana Rw2G054470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 44, 328
AccBSI CCGCTC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 1549
AccII CGCG 1 cut(s) 279
AciI CCGC 9 cut(s) 5, 28, 51, 156, 232, 270, 274, 277, 423
AclI AACGTT 1 cut(s) 1008
AclWI GGATC 4 cut(s) 1172, 1185, 1724, 1816
AcsI RAATTY 3 cut(s) 728, 880, 1608
AcuI CTGAAG 1 cut(s) 392
AcyI GRCGYC 2 cut(s) 45, 329
AfaI GTAC 3 cut(s) 392, 1984, 1988
AfiI CCNNNNNNNGG 3 cut(s) 138, 1298, 1328
AgsI TTSAA 6 cut(s) 489, 668, 677, 1753, 1761, 1972
AjnI CCWGG 3 cut(s) 1291, 1321, 1712
AloI GAACNNNNNNTCC 2 cut(s) 1733, 1765
Alw21I GWGCWC 6 cut(s) 129, 285, 721, 1447, 1539, 1980
Alw26I GTCTC 2 cut(s) 182, 895
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 4 cut(s) 1172, 1185, 1724, 1816
AlwNI CAGNNNCTG 1 cut(s) 1919
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 5 cut(s) 169, 573, 1250, 1300, 1955
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 1 cut(s) 1655
ApoI RAATTY 3 cut(s) 728, 880, 1608
Asp700I GAANNNNTTC 1 cut(s) 618
AspLEI GCGC 3 cut(s) 47, 331, 865
AspS9I GGNCC 3 cut(s) 1094, 1316, 1847
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 5 cut(s) 867, 1070, 1439, 1544, 1840
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 3 cut(s) 1094, 1316, 1847
BaeGI GKGCMC 1 cut(s) 721
BamHI GGATCC 1 cut(s) 1177
BanI GGYRCC 2 cut(s) 44, 328
BanII GRGCYC 4 cut(s) 129, 285, 967, 1980
BarI GAAGNNNNNNTAC 2 cut(s) 603, 635
BauI CACGAG 1 cut(s) 1026
BbsI GAAGAC 2 cut(s) 201, 1133
Bbv12I GWGCWC 6 cut(s) 129, 285, 721, 1447, 1539, 1980
BbvI GCAGC 1 cut(s) 1667
BccI CCATC 9 cut(s) 532, 749, 772, 785, 1094, 1185, 1234, 1573, 1736
BceAI ACGGC 1 cut(s) 604
BciT130I CCWGG 3 cut(s) 1293, 1323, 1714
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 182, 895
BfaI CTAG 5 cut(s) 417, 477, 579, 735, 1436
BfoI RGCGCY 3 cut(s) 48, 332, 866
BisI GCNGC 5 cut(s) 29, 52, 270, 277, 1656
BlsI GCNGC 5 cut(s) 30, 53, 271, 278, 1657
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 4 cut(s) 333, 1293, 1323, 1714
Bme18I GGWCC 3 cut(s) 1094, 1316, 1847
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 3 cut(s) 1094, 1316, 1847
BmiI GGNNCC 8 cut(s) 46, 101, 121, 330, 964, 1134, 1179, 1848
BmrFI CCNGG 4 cut(s) 333, 1293, 1323, 1714
BmsI GCATC 3 cut(s) 769, 1039, 1825
BpiI GAAGAC 2 cut(s) 201, 1133
BplI GAGNNNNNCTC 4 cut(s) 111, 143, 1846, 1878
BpmI CTGGAG 2 cut(s) 88, 1380
BpuEI CTTGAG 1 cut(s) 1166
BpuMI CCSGG 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 1986
BsaHI GRCGYC 2 cut(s) 45, 329
BsaJI CCNNGG 5 cut(s) 181, 561, 967, 1292, 1795
BsaXI ACNNNNNCTCC 4 cut(s) 134, 137, 164, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 1298, 1328
Bse118I RCCGGY 2 cut(s) 41, 47
Bse1I ACTGG 2 cut(s) 1395, 1913
Bse3DI GCAATG 1 cut(s) 1940
BseBI CCWGG 3 cut(s) 1293, 1323, 1714
BseDI CCNNGG 5 cut(s) 181, 561, 967, 1292, 1795
BseGI GGATG 4 cut(s) 228, 844, 1747, 1840
BseLI CCNNNNNNNGG 3 cut(s) 138, 1298, 1328
BseMI GCAATG 1 cut(s) 1940
BseMII CTCAG 5 cut(s) 173, 218, 585, 1818, 1844
BseNI ACTGG 2 cut(s) 1395, 1913
BseRI GAGGAG 3 cut(s) 48, 142, 885
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 1 cut(s) 1667
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 2 cut(s) 42, 301
BshFI GGCC 5 cut(s) 171, 575, 1252, 1302, 1957
BshNI GGYRCC 2 cut(s) 44, 328
BsiEI CGRYCG 2 cut(s) 42, 301
BsiHKAI GWGCWC 6 cut(s) 129, 285, 721, 1447, 1539, 1980
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 3 cut(s) 42, 48, 332
BsiWI CGTACG 1 cut(s) 1986
BslFI GGGAC 2 cut(s) 745, 965
BslI CCNNNNNNNGG 3 cut(s) 138, 1298, 1328
BsmAI GTCTC 2 cut(s) 182, 895
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 745, 965
BsnI GGCC 5 cut(s) 171, 575, 1252, 1302, 1957
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 7 cut(s) 129, 285, 721, 967, 1447, 1539, 1980
Bsp143I GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
BspACI CCGC 9 cut(s) 5, 28, 51, 156, 232, 270, 274, 277, 423
BspANI GGCC 5 cut(s) 171, 575, 1252, 1302, 1957
BspCNI CTCAG 5 cut(s) 172, 217, 586, 1817, 1845
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 8 cut(s) 46, 101, 121, 330, 964, 1134, 1179, 1848
BspPI GGATC 4 cut(s) 1172, 1185, 1724, 1816
BspQI GCTCTTC 1 cut(s) 408
BspT107I GGYRCC 2 cut(s) 44, 328
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 1940
BsrFI RCCGGY 2 cut(s) 41, 47
BsrI ACTGG 2 cut(s) 1395, 1913
BssAI RCCGGY 2 cut(s) 41, 47
BssECI CCNNGG 5 cut(s) 181, 561, 967, 1292, 1795
BssMI GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
BssNI GRCGYC 2 cut(s) 45, 329
BssSI CACGAG 1 cut(s) 1026
BssT1I CCWWGG 3 cut(s) 181, 561, 1795
Bst2BI CACGAG 1 cut(s) 1026
Bst2UI CCWGG 3 cut(s) 1293, 1323, 1714
Bst4CI ACNGT 5 cut(s) 1449, 1595, 1749, 1921, 2025
Bst6I CTCTTC 5 cut(s) 51, 408, 664, 672, 847
BstACI GRCGYC 2 cut(s) 45, 329
BstBAI YACGTR 1 cut(s) 1986
BstDEI CTNAG 9 cut(s) 159, 204, 594, 756, 1068, 1121, 1732, 1804, 1853
BstDSI CCRYGG 1 cut(s) 967
BstENI CCTNNNNNAGG 1 cut(s) 1296
BstF5I GGATG 4 cut(s) 228, 844, 1747, 1840
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 3 cut(s) 48, 332, 866
BstHHI GCGC 3 cut(s) 47, 331, 865
BstKTI GATC 7 cut(s) 301, 451, 875, 941, 1180, 1719, 1811
BstMAI GTCTC 2 cut(s) 182, 895
BstMBI GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
BstMCI CGRYCG 2 cut(s) 42, 301
BstMWI GCNNNNNNNGC 6 cut(s) 44, 51, 128, 326, 716, 1364
BstNI CCWGG 3 cut(s) 1293, 1323, 1714
BstNSI RCATGY 2 cut(s) 1154, 1220
BstSCI CCNGG 4 cut(s) 331, 1291, 1321, 1712
BstSLI GKGCMC 1 cut(s) 721
BstSNI TACGTA 1 cut(s) 1986
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 1667
BstV2I GAAGAC 2 cut(s) 201, 1133
BstX2I RGATCY 1 cut(s) 1177
BstXI CCANNNNNNTGG 1 cut(s) 644
BstYI RGATCY 1 cut(s) 1177
BsuRI GGCC 5 cut(s) 171, 575, 1252, 1302, 1957
BtgI CCRYGG 1 cut(s) 967
BtsCI GGATG 4 cut(s) 228, 844, 1747, 1840
BtsIMutI CAGTG 3 cut(s) 1445, 1600, 1850
CaiI CAGNNNCTG 1 cut(s) 1919
CfoI GCGC 3 cut(s) 47, 331, 865
Cfr10I RCCGGY 2 cut(s) 41, 47
Cfr13I GGNCC 3 cut(s) 1094, 1316, 1847
CseI GACGC 1 cut(s) 1898
Csp6I GTAC 3 cut(s) 391, 1983, 1987
CviAII CATG 3 cut(s) 1151, 1217, 1816
CviQI GTAC 3 cut(s) 391, 1983, 1987
DdeI CTNAG 9 cut(s) 159, 204, 594, 756, 1068, 1121, 1732, 1804, 1853
DinI GGCGCC 2 cut(s) 46, 330
DpnI GATC 7 cut(s) 300, 450, 874, 940, 1179, 1718, 1810
DpnII GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
Eam1104I CTCTTC 5 cut(s) 51, 408, 664, 672, 847
EarI CTCTTC 5 cut(s) 51, 408, 664, 672, 847
EciI GGCGGA 1 cut(s) 221
Ecl136II GAGCTC 3 cut(s) 127, 283, 1978
Eco105I TACGTA 1 cut(s) 1986
Eco130I CCWWGG 3 cut(s) 181, 561, 1795
Eco147I AGGCCT 2 cut(s) 1302, 1957
Eco24I GRGCYC 4 cut(s) 129, 285, 967, 1980
Eco47I GGWCC 3 cut(s) 1094, 1316, 1847
Eco53kI GAGCTC 3 cut(s) 127, 283, 1978
Eco57I CTGAAG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 3 cut(s) 127, 283, 1978
EcoNI CCTNNNNNAGG 1 cut(s) 1296
EcoRII CCWGG 3 cut(s) 1291, 1321, 1712
EcoT14I CCWWGG 3 cut(s) 181, 561, 1795
EcoT22I ATGCAT 1 cut(s) 1054
EcoT38I GRGCYC 4 cut(s) 129, 285, 967, 1980
EgeI GGCGCC 2 cut(s) 46, 330
EheI GGCGCC 2 cut(s) 46, 330
ErhI CCWWGG 3 cut(s) 181, 561, 1795
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 3 cut(s) 1154, 1220, 1819
FalI AAGNNNNNCTT 2 cut(s) 1290, 1322
FaqI GGGAC 2 cut(s) 745, 965
FatI CATG 3 cut(s) 1150, 1216, 1815
FauI CCCGC 1 cut(s) 281
FauNDI CATATG 3 cut(s) 943, 1247, 1781
FblI GTMKAC 1 cut(s) 1549
Fnu4HI GCNGC 5 cut(s) 29, 52, 270, 277, 1656
FokI GGATG 4 cut(s) 215, 851, 1754, 1847
FriOI GRGCYC 4 cut(s) 129, 285, 967, 1980
Fsp4HI GCNGC 5 cut(s) 29, 52, 270, 277, 1656
FspBI CTAG 5 cut(s) 417, 477, 579, 735, 1436
GlaI GCGC 3 cut(s) 46, 330, 864
GluI GCNGC 5 cut(s) 29, 52, 270, 277, 1656
GsuI CTGGAG 2 cut(s) 88, 1380
HaeII RGCGCY 3 cut(s) 48, 332, 866
HaeIII GGCC 5 cut(s) 171, 575, 1252, 1302, 1957
HapII CCGG 3 cut(s) 42, 48, 332
HgaI GACGC 1 cut(s) 1898
HhaI GCGC 3 cut(s) 47, 331, 865
Hin1I GRCGYC 2 cut(s) 45, 329
Hin1II CATG 3 cut(s) 1154, 1220, 1819
Hin6I GCGC 3 cut(s) 45, 329, 863
HinP1I GCGC 3 cut(s) 45, 329, 863
HincII GTYRAC 2 cut(s) 474, 513
HindII GTYRAC 2 cut(s) 474, 513
HindIII AAGCTT 2 cut(s) 617, 2014
HinfI GANTC 9 cut(s) 163, 194, 1029, 1061, 1455, 1502, 1541, 1554, 1726
HpaII CCGG 3 cut(s) 42, 48, 332
HphI GGTGA 5 cut(s) 867, 1070, 1439, 1544, 1840
Hpy166II GTNNAC 7 cut(s) 82, 474, 513, 719, 1319, 1550, 1850
Hpy188I TCNGA 8 cut(s) 119, 162, 298, 595, 1045, 1528, 1813, 1866
Hpy8I GTNNAC 7 cut(s) 82, 474, 513, 719, 1319, 1550, 1850
Hpy99I CGWCG 1 cut(s) 246
HpyCH4III ACNGT 5 cut(s) 1449, 1595, 1749, 1921, 2025
HpyCH4IV ACGT 4 cut(s) 715, 1008, 1261, 1985
HpyCH4V TGCA 5 cut(s) 719, 1052, 1785, 1940, 1945
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 51, 128, 326, 716, 1364
HpyF3I CTNAG 9 cut(s) 159, 204, 594, 756, 1068, 1121, 1732, 1804, 1853
HpySE526I ACGT 4 cut(s) 715, 1008, 1261, 1985
Hsp92I GRCGYC 2 cut(s) 45, 329
Hsp92II CATG 3 cut(s) 1154, 1220, 1819
HspAI GCGC 3 cut(s) 45, 329, 863
KasI GGCGCC 2 cut(s) 44, 328
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Kzo9I GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 7 cut(s) 35, 105, 119, 686, 962, 1213, 1622
Lsp1109I GCAGC 1 cut(s) 1667
LweI GCATC 3 cut(s) 769, 1039, 1825
MaeI CTAG 5 cut(s) 417, 477, 579, 735, 1436
MaeII ACGT 4 cut(s) 715, 1008, 1261, 1985
MaeIII GTNAC 8 cut(s) 584, 811, 1018, 1058, 1231, 1799, 1915, 2019
MalI GATC 7 cut(s) 300, 450, 874, 940, 1179, 1718, 1810
MbiI CCGCTC 1 cut(s) 158
MboI GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
MfeI CAATTG 1 cut(s) 452
MflI RGATCY 1 cut(s) 1177
MhlI GDGCHC 7 cut(s) 129, 285, 721, 967, 1447, 1539, 1980
MluCI AATT 9 cut(s) 452, 728, 827, 880, 973, 1497, 1608, 1786, 2006
Mly113I GGCGCC 2 cut(s) 45, 329
MlyI GAGTC 5 cut(s) 188, 1038, 1055, 1464, 1548
Mph1103I ATGCAT 1 cut(s) 1054
MreI CGCCGGCG 1 cut(s) 47
MroNI GCCGGC 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 618
MseI TTAA 5 cut(s) 378, 990, 1275, 1403, 1790
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 1478
MspI CCGG 3 cut(s) 42, 48, 332
MspR9I CCNGG 4 cut(s) 333, 1293, 1323, 1714
MunI CAATTG 1 cut(s) 452
MvaI CCWGG 3 cut(s) 1293, 1323, 1714
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 6 cut(s) 44, 51, 128, 326, 716, 1364
NaeI GCCGGC 1 cut(s) 49
NarI GGCGCC 2 cut(s) 45, 329
NciI CCSGG 1 cut(s) 333
NdeI CATATG 3 cut(s) 943, 1247, 1781
NdeII GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 3 cut(s) 1154, 1220, 1819
NlaIV GGNNCC 8 cut(s) 46, 101, 121, 330, 964, 1134, 1179, 1848
NmeAIII GCCGAG 2 cut(s) 147, 148
NmuCI GTSAC 3 cut(s) 1018, 1058, 2019
NsiI ATGCAT 1 cut(s) 1054
NspI RCATGY 2 cut(s) 1154, 1220
PaeI GCATGC 1 cut(s) 1220
PaeR7I CTCGAG 1 cut(s) 134
PceI AGGCCT 2 cut(s) 1302, 1957
PciSI GCTCTTC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 3 cut(s) 141, 964, 1986
PdiI GCCGGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 4 cut(s) 163, 1502, 1541, 1726
Pfl23II CGTACG 1 cut(s) 1986
PkrI GCNGC 5 cut(s) 30, 53, 271, 278, 1657
Ple19I CGATCG 1 cut(s) 301
PleI GAGTC 5 cut(s) 188, 1037, 1055, 1463, 1548
PluTI GGCGCC 2 cut(s) 48, 332
PpsI GAGTC 5 cut(s) 188, 1037, 1055, 1463, 1548
Ppu21I YACGTR 1 cut(s) 1986
Psp124BI GAGCTC 3 cut(s) 129, 285, 1980
Psp1406I AACGTT 1 cut(s) 1008
Psp6I CCWGG 3 cut(s) 1291, 1321, 1712
PspGI CCWGG 3 cut(s) 1291, 1321, 1712
PspLI CGTACG 1 cut(s) 1986
PspN4I GGNNCC 8 cut(s) 46, 101, 121, 330, 964, 1134, 1179, 1848
PspPI GGNCC 3 cut(s) 1094, 1316, 1847
PstNI CAGNNNCTG 1 cut(s) 1919
PsuI RGATCY 1 cut(s) 1177
PvuI CGATCG 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 1478
RsaI GTAC 3 cut(s) 392, 1984, 1988
RsaNI GTAC 3 cut(s) 391, 1983, 1987
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 3 cut(s) 129, 285, 1980
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 5 cut(s) 378, 990, 1275, 1403, 1790
SatI GCNGC 5 cut(s) 29, 52, 270, 277, 1656
Sau3AI GATC 7 cut(s) 298, 448, 872, 938, 1177, 1716, 1808
Sau96I GGNCC 3 cut(s) 1094, 1316, 1847
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 5 cut(s) 188, 1038, 1055, 1464, 1548
ScrFI CCNGG 4 cut(s) 333, 1293, 1323, 1714
SduI GDGCHC 7 cut(s) 129, 285, 721, 967, 1447, 1539, 1980
SfaNI GCATC 3 cut(s) 769, 1039, 1825
SfoI GGCGCC 2 cut(s) 46, 330
Sfr274I CTCGAG 1 cut(s) 134
SgrAI CRCCGGYG 1 cut(s) 47
SinI GGWCC 3 cut(s) 1094, 1316, 1847
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 134, 1145
SmoI CTYRAG 2 cut(s) 134, 1145
SnaBI TACGTA 1 cut(s) 1986
SphI GCATGC 1 cut(s) 1220
Sse9I AATT 9 cut(s) 452, 728, 827, 880, 973, 1497, 1608, 1786, 2006
SseBI AGGCCT 2 cut(s) 1302, 1957
SsiI CCGC 9 cut(s) 5, 28, 51, 156, 232, 270, 274, 277, 423
SspDI GGCGCC 2 cut(s) 44, 328
SspMI CTAG 5 cut(s) 417, 477, 579, 735, 1436
SstI GAGCTC 3 cut(s) 129, 285, 1980
StuI AGGCCT 2 cut(s) 1302, 1957
StyD4I CCNGG 4 cut(s) 331, 1291, 1321, 1712
StyI CCWWGG 3 cut(s) 181, 561, 1795
TaaI ACNGT 5 cut(s) 1449, 1595, 1749, 1921, 2025
TaiI ACGT 4 cut(s) 718, 1011, 1264, 1988
TaqI TCGA 7 cut(s) 135, 192, 216, 244, 536, 1189, 1878
TasI AATT 9 cut(s) 452, 728, 827, 880, 973, 1497, 1608, 1786, 2006
TatI WGTACW 1 cut(s) 390
TauI GCSGC 4 cut(s) 31, 54, 272, 279
TfiI GAWTC 4 cut(s) 163, 1502, 1541, 1726
Tru1I TTAA 5 cut(s) 378, 990, 1275, 1403, 1790
Tru9I TTAA 5 cut(s) 378, 990, 1275, 1403, 1790
TscAI CASTG 3 cut(s) 1452, 1600, 1857
TseFI GTSAC 3 cut(s) 1018, 1058, 2019
TseI GCWGC 1 cut(s) 1655
Tsp45I GTSAC 3 cut(s) 1018, 1058, 2019
TspDTI ATGAA 6 cut(s) 217, 1389, 1509, 1665, 1689, 1832
TspGWI ACGGA 2 cut(s) 1675, 1738
TspRI CASTG 3 cut(s) 1452, 1600, 1857
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 3 cut(s) 1094, 1316, 1847
XagI CCTNNNNNAGG 1 cut(s) 1296
XapI RAATTY 3 cut(s) 728, 880, 1608
XbaI TCTAGA 1 cut(s) 578
XceI RCATGY 2 cut(s) 1154, 1220
XcmI CCANNNNNNNNNTGG 4 cut(s) 568, 638, 826, 832
XhoI CTCGAG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 1549
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 5 cut(s) 417, 477, 579, 735, 1436
ZrmI AGTACT 1 cut(s) 392
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.