Rmu_sc0003302.1_g000002

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003302.1
OG0012914
Physical Location & Seq
Forward (+)
3382 .. 7502
4121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003302.1_g000002.1.cds

Sequence Viewer

Length: 2022 bp
atggcgggaaaggaggaaccgaactccggcgaagaggtctgctggagaaagcaagtcggcgataacgtaaaccgccttcagtcccttctcttcggagccgaactcgccgccgagagaaatgacttcttctccgctcagatattctctcttcgtctcctcggctttctcgactcacagtctcactctcagctcgacggcgctttcgtcagccctattcgccgcgaagtcgtctccaagctcggagaggcccgtcgtgccctcacttctgattccgatcgcaaagctttcgagcaagcgaaaaaagatcctggttttgttttcagcacaaagggagatattgacattgggaggattaaacagtcaaagtactttcaggctcttcttcagcagcctaacaatggaaaggttgtgaatgatttgggagatcaactggagaggcgggacaaattggctagcaagttatcaaaagctatgacacaaactaagttggcatcattgtacaaaaacaacattgggacggcaagtaaggacacatcccgtagttcttgggctcctaaaagtaacagctctgaggactgcactattgtggaaaagcgtcactctcatcacaaccgtaacagcagtcatactacctcatcttttcaaaaagttgaagaggaagaaagagctcatgggtatcccttaggccctaaacgtgtacacagggaatttaatagccccattattgataatatgaagtccccatcagccaatgaagatgctaaagccgatgtttctggcaatggatttgttactgccaaagccaaattggaaatggatgtgaggcaaaggcgagggttagctgcatcatcaaatacttctgtctctccacaaagtgataacagtgttgctaataggggttttagcacgagatcatatggtgtttcacgtcgaggtgtccgcggtaattttattcctcccgtcagatctaatgggggcaatgttgggaatatgacttcaaaaattggtggaaagtcggatgatgcattagatgactcaactaggagctgtatggaaatgctcagcggtcctgatggggagctcccagaaaaattaaggaacttggaacctcgtcttattgaacatgtcagtaatgagattatggacaaggatccaaatgttcgatggaatgatattgctggcttggatcatgctaaaaagtgtgtgacggagatggttatatggcccctgttacgtcccgacattttcaaaggatgtcgttctcctggaaagggtcttcttctcttcggtccaccaggaacaggtaaaacaatgattgggaaagccatagctggagaggcaaaagcaacgttcttctacatctctgccagttcattgacgagcaaatggattggtgagggtgaaaagttagttcgggccctttttggagtagccagttgtcggcagccagctgtaatttttgttgatgaaatcgactcacttctctcacaggtaagacttgctctagctcttgaagttaaactggacatgcggaagtcagaaggtgaacatgaatcaagtaggcgactcaaaacacagtttcttattgaaatggaaggctttgacagtggcagggcaacaaatcgaccccaagagcttgatgaagcagcacgtaggcgacttactaagagactttacattccgctgcctgcatcagaagcgagagcctggatagtggagaatctcctggagaaggatggtctattcaaactttcaagggaagatattgataacatatgcaaattaacagaagggtactcaggatcagacatgaaaaacttagtgaaggatgcctctatgggtcctctaagagaagctctgagacaaggcatagaaataacaaatcttaagaaggaggatatgcgcccggtaacttttcaggattttgagagtgcattacatgaggtgaggccctctgtttccttgaatgagcttggtacctatgatgattggaacaagcaatttggaagtttgtccctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000228 GO:0000280 GO:0000287 GO:0001503 GO:0001649 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006163 GO:0006282 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007140 GO:0007276 GO:0008150 GO:0008152 GO:0008283 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009314 GO:0009628 GO:0009892 GO:0009987 GO:0010212 GO:0010520 GO:0010564 GO:0010569 GO:0010605 GO:0010639 GO:0010941 GO:0010948 GO:0016043 GO:0016787 GO:0017144 GO:0019219 GO:0019222 GO:0019637 GO:0019693 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0033687 GO:0034641 GO:0040020 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045128 GO:0045786 GO:0045835 GO:0045910 GO:0045934 GO:0046034 GO:0046483 GO:0046872 GO:0048232 GO:0048285 GO:0048471 GO:0048519 GO:0048523 GO:0048583 GO:0048609 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051321 GO:0051445 GO:0051447 GO:0051704 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0055086 GO:0060255 GO:0060548 GO:0060631 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0071840 GO:0072521 GO:0080090 GO:0080134 GO:0080135 GO:0104004 GO:0110029 GO:0140013 GO:1901135 GO:1901360 GO:1901564 GO:1903046 GO:2000241 GO:2000242 GO:2000779 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Vps4_C

Protein Analysis

673

Amino Acids

74.69

Weight (kDa)

8.88

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012914)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27120
fragaria_vesca FvH4_6g53320
malus_domestica MD09G1011500.v1.1 MD17G1012000.v1.1
prunus_persica Prupe.3G305500_v2.0.a1
pyrus_communis pycom111g00980 pycom17g00970
rosa_chinensis RchiOBHm_Chr2g0175481
rosa_laevigata RLG00000022331
rosa_multiflora Rmu_sc0003302.1_g000002 Rmu_sc0021955.1_g000001
rosa_roxburghii Rroxscaffold_2G00077280
rosa_rugosa Rorug02G0585100
rosa_samantha Rh2AG664600 Rh2BG675300 Rh2CG638500 Rh2DG689300
rosa_wichuraiana Rw2G054470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1976
AccB1I GGYRCC 1 cut(s) 1976
AccBSI CCGCTC 1 cut(s) 134
AccII CGCG 2 cut(s) 222, 942
AclI AACGTT 1 cut(s) 1349
AclWI GGATC 5 cut(s) 299, 1145, 1158, 1194, 1810
AcsI RAATTY 1 cut(s) 707
AcuI CTGAAG 2 cut(s) 62, 368
AdeI CACNNNGTG 1 cut(s) 875
AfaI GTAC 5 cut(s) 368, 500, 699, 1796, 1978
AfiI CCNNNNNNNGG 6 cut(s) 26, 398, 1271, 1301, 1440, 1732
AflII CTTAAG 1 cut(s) 1886
AflIII ACRYGT 2 cut(s) 694, 1123
AhdI GACNNNNNGTC 1 cut(s) 175
AjiI CACGTC 1 cut(s) 929
AjnI CCWGG 5 cut(s) 307, 1264, 1294, 1706, 1725
AleI CACNNNNGTG 1 cut(s) 584
Alw21I GWGCWC 2 cut(s) 670, 1083
Alw26I GTCTC 6 cut(s) 158, 183, 235, 868, 1663, 1855
AlwI GGATC 5 cut(s) 299, 1145, 1158, 1194, 1810
AoxI GGCC 5 cut(s) 246, 685, 1223, 1416, 1949
ApaI GGGCCC 1 cut(s) 1420
ApeKI GCWGC 5 cut(s) 388, 842, 1444, 1646, 1684
ApoI RAATTY 1 cut(s) 707
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
Asp718I GGTACC 1 cut(s) 1976
AspLEI GCGC 2 cut(s) 200, 1905
AspS9I GGNCC 9 cut(s) 247, 686, 1067, 1224, 1289, 1416, 1417, 1841, 1950
AsuC2I CCSGG 1 cut(s) 1907
AsuHPI GGTGA 4 cut(s) 1406, 1412, 1556, 1957
AsuNHI GCTAGC 1 cut(s) 452
AvaII GGWCC 3 cut(s) 1067, 1289, 1841
AxyI CCTNAGG 1 cut(s) 682
BaeGI GKGCMC 2 cut(s) 259, 1420
BamHI GGATCC 1 cut(s) 1150
BanI GGYRCC 1 cut(s) 1976
BanII GRGCYC 4 cut(s) 553, 670, 1083, 1420
BarI GAAGNNNNNNTAC 2 cut(s) 60, 92
BauI CACGAG 1 cut(s) 907
BbsI GAAGAC 1 cut(s) 1268
Bbv12I GWGCWC 2 cut(s) 670, 1083
BbvI GCAGC 5 cut(s) 400, 829, 1456, 1658, 1671
BccI CCATC 5 cut(s) 751, 1067, 1158, 1207, 1730
BceAI ACGGC 2 cut(s) 211, 534
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 5 cut(s) 309, 1266, 1296, 1708, 1727
BciVI GTATCC 1 cut(s) 687
BcnI CCSGG 1 cut(s) 1907
BcoDI GTCTC 6 cut(s) 158, 183, 235, 868, 1663, 1855
BfaI CTAG 3 cut(s) 453, 1041, 1505
BfoI RGCGCY 1 cut(s) 201
BfrI CTTAAG 1 cut(s) 1886
BfuI GTATCC 1 cut(s) 687
BglII AGATCT 1 cut(s) 965
BisI GCNGC 7 cut(s) 108, 220, 389, 843, 1445, 1647, 1685
BlpI GCTNAGC 1 cut(s) 1061
BlsI GCNGC 7 cut(s) 109, 221, 390, 844, 1446, 1648, 1686
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 6 cut(s) 309, 1266, 1296, 1708, 1727, 1907
Bme18I GGWCC 3 cut(s) 1067, 1289, 1841
BmeRI GACNNNNNGTC 1 cut(s) 175
BmgBI CACGTC 1 cut(s) 929
BmgT120I GGNCC 9 cut(s) 247, 686, 1067, 1224, 1289, 1416, 1417, 1841, 1950
BmiI GGNNCC 9 cut(s) 18, 97, 552, 1107, 1152, 1226, 1418, 1842, 1978
BmrFI CCNGG 6 cut(s) 309, 1266, 1296, 1708, 1727, 1907
BmsI GCATC 6 cut(s) 500, 748, 854, 1012, 1700, 1819
BmtI GCTAGC 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 1268
BplI GAGNNNNNCTC 2 cut(s) 87, 119
BpmI CTGGAG 4 cut(s) 64, 452, 1353, 1748
Bpu1102I GCTNAGC 1 cut(s) 1061
BpuMI CCSGG 1 cut(s) 1907
BsaAI YACGTR 1 cut(s) 1652
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 157, 940
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 398, 1271, 1301, 1440, 1732
Bse1I ACTGG 4 cut(s) 435, 1368, 1434, 1527
Bse21I CCTNAGG 1 cut(s) 682
Bse3DI GCAATG 2 cut(s) 787, 985
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 5 cut(s) 309, 1266, 1296, 1708, 1727
BseDI CCNNGG 2 cut(s) 157, 940
BseGI GGATG 6 cut(s) 533, 823, 1024, 1259, 1741, 1834
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 6 cut(s) 26, 398, 1271, 1301, 1440, 1732
BseMI GCAATG 2 cut(s) 787, 985
BseMII CTCAG 6 cut(s) 149, 200, 561, 1075, 1812, 1850
BseNI ACTGG 4 cut(s) 435, 1368, 1434, 1527
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 2 cut(s) 259, 1420
BseXI GCAGC 5 cut(s) 400, 829, 1456, 1658, 1671
BsgI GTGCAG 1 cut(s) 562
Bsh1236I CGCG 2 cut(s) 222, 942
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 5 cut(s) 248, 687, 1225, 1418, 1951
BshNI GGYRCC 1 cut(s) 1976
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 2 cut(s) 670, 1083
BsiSI CCGG 2 cut(s) 27, 1907
BslFI GGGAC 6 cut(s) 67, 455, 529, 724, 1221, 1999
BslI CCNNNNNNNGG 6 cut(s) 26, 398, 1271, 1301, 1440, 1732
BsmAI GTCTC 6 cut(s) 158, 183, 235, 868, 1663, 1855
BsmBI CGTCTC 2 cut(s) 158, 235
BsmFI GGGAC 6 cut(s) 67, 455, 529, 724, 1221, 1999
BsnI GGCC 5 cut(s) 248, 687, 1225, 1418, 1951
Bsp120I GGGCCC 1 cut(s) 1416
Bsp1286I GDGCHC 5 cut(s) 259, 553, 670, 1083, 1420
Bsp1407I TGTACA 2 cut(s) 498, 697
Bsp143I GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
Bsp1720I GCTNAGC 1 cut(s) 1061
BspANI GGCC 5 cut(s) 248, 687, 1225, 1418, 1951
BspCNI CTCAG 6 cut(s) 148, 199, 562, 1074, 1811, 1851
BspFNI CGCG 2 cut(s) 222, 942
BspLI GGNNCC 9 cut(s) 18, 97, 552, 1107, 1152, 1226, 1418, 1842, 1978
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 5 cut(s) 299, 1145, 1158, 1194, 1810
BspQI GCTCTTC 1 cut(s) 384
BspT107I GGYRCC 1 cut(s) 1976
BspTI CTTAAG 1 cut(s) 1886
BsrBI CCGCTC 1 cut(s) 134
BsrDI GCAATG 2 cut(s) 787, 985
BsrGI TGTACA 2 cut(s) 498, 697
BsrI ACTGG 4 cut(s) 435, 1368, 1434, 1527
BssECI CCNNGG 2 cut(s) 157, 940
BssMI GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
BssSI CACGAG 1 cut(s) 907
Bst2BI CACGAG 1 cut(s) 907
Bst2UI CCWGG 5 cut(s) 309, 1266, 1296, 1708, 1727
Bst4CI ACNGT 6 cut(s) 177, 360, 614, 884, 1578, 1607
Bst6I CTCTTC 6 cut(s) 27, 95, 153, 384, 648, 1289
BstAFI CTTAAG 1 cut(s) 1886
BstAUI TGTACA 2 cut(s) 498, 697
BstBAI YACGTR 1 cut(s) 1652
BstC8I GCNNGC 5 cut(s) 294, 454, 1180, 1449, 1689
BstDSI CCRYGG 1 cut(s) 940
BstENI CCTNNNNNAGG 1 cut(s) 1730
BstF5I GGATG 6 cut(s) 533, 823, 1024, 1259, 1741, 1834
BstFNI CGCG 2 cut(s) 222, 942
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 2 cut(s) 200, 1905
BstKTI GATC 8 cut(s) 277, 307, 427, 914, 968, 1153, 1189, 1805
BstMAI GTCTC 6 cut(s) 158, 183, 235, 868, 1663, 1855
BstMBI GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 5 cut(s) 104, 216, 254, 1337, 1697
BstNI CCWGG 5 cut(s) 309, 1266, 1296, 1708, 1727
BstNSI RCATGY 2 cut(s) 1127, 1531
BstSCI CCNGG 6 cut(s) 307, 1264, 1294, 1706, 1725, 1905
BstSLI GKGCMC 2 cut(s) 259, 1420
BstUI CGCG 2 cut(s) 222, 942
BstV1I GCAGC 5 cut(s) 400, 829, 1456, 1658, 1671
BstV2I GAAGAC 1 cut(s) 1268
BstX2I RGATCY 3 cut(s) 304, 965, 1150
BstYI RGATCY 3 cut(s) 304, 965, 1150
Bsu36I CCTNAGG 1 cut(s) 682
BsuI GTATCC 1 cut(s) 687
BsuRI GGCC 5 cut(s) 248, 687, 1225, 1418, 1951
BtgI CCRYGG 1 cut(s) 940
BtrI CACGTC 1 cut(s) 929
BtsCI GGATG 6 cut(s) 533, 823, 1024, 1259, 1741, 1834
BtsIMutI CAGTG 2 cut(s) 889, 1612
Cac8I GCNNGC 5 cut(s) 294, 454, 1180, 1449, 1689
CfoI GCGC 2 cut(s) 200, 1905
Cfr13I GGNCC 9 cut(s) 247, 686, 1067, 1224, 1289, 1416, 1417, 1841, 1950
Cfr42I CCGCGG 1 cut(s) 943
CseI GACGC 1 cut(s) 584
Csp6I GTAC 5 cut(s) 367, 499, 698, 1795, 1977
CviAII CATG 7 cut(s) 671, 1124, 1190, 1528, 1550, 1810, 1940
CviQI GTAC 5 cut(s) 367, 499, 698, 1795, 1977
DpnI GATC 8 cut(s) 276, 306, 426, 913, 967, 1152, 1188, 1804
DpnII GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
DraIII CACNNNGTG 1 cut(s) 875
DriI GACNNNNNGTC 1 cut(s) 175
Eam1104I CTCTTC 6 cut(s) 27, 95, 153, 384, 648, 1289
Eam1105I GACNNNNNGTC 1 cut(s) 175
EarI CTCTTC 6 cut(s) 27, 95, 153, 384, 648, 1289
Ecl136II GAGCTC 2 cut(s) 668, 1081
Eco24I GRGCYC 4 cut(s) 553, 670, 1083, 1420
Eco47I GGWCC 3 cut(s) 1067, 1289, 1841
Eco53kI GAGCTC 2 cut(s) 668, 1081
Eco57I CTGAAG 2 cut(s) 62, 368
Eco81I CCTNAGG 1 cut(s) 682
EcoICRI GAGCTC 2 cut(s) 668, 1081
EcoNI CCTNNNNNAGG 1 cut(s) 1730
EcoO109I RGGNCCY 4 cut(s) 686, 1417, 1841, 1950
EcoRII CCWGG 5 cut(s) 307, 1264, 1294, 1706, 1725
EcoT22I ATGCAT 1 cut(s) 1027
EcoT38I GRGCYC 4 cut(s) 553, 670, 1083, 1420
Esp3I CGTCTC 2 cut(s) 158, 235
FaeI CATG 7 cut(s) 674, 1127, 1193, 1531, 1553, 1813, 1943
FaqI GGGAC 6 cut(s) 67, 455, 529, 724, 1221, 1999
FatI CATG 7 cut(s) 670, 1123, 1189, 1527, 1549, 1809, 1939
FauI CCCGC 1 cut(s) 432
FauNDI CATATG 2 cut(s) 916, 1775
Fnu4HI GCNGC 7 cut(s) 108, 220, 389, 843, 1445, 1647, 1685
FokI GGATG 6 cut(s) 520, 830, 1031, 1266, 1748, 1841
FriOI GRGCYC 4 cut(s) 553, 670, 1083, 1420
Fsp4HI GCNGC 7 cut(s) 108, 220, 389, 843, 1445, 1647, 1685
FspBI CTAG 3 cut(s) 453, 1041, 1505
GlaI GCGC 2 cut(s) 199, 1904
GluI GCNGC 7 cut(s) 108, 220, 389, 843, 1445, 1647, 1685
GsuI CTGGAG 4 cut(s) 64, 452, 1353, 1748
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 5 cut(s) 248, 687, 1225, 1418, 1951
HapII CCGG 2 cut(s) 27, 1907
HgaI GACGC 1 cut(s) 584
HhaI GCGC 2 cut(s) 200, 1905
Hin1II CATG 7 cut(s) 674, 1127, 1193, 1531, 1553, 1813, 1943
Hin6I GCGC 2 cut(s) 198, 1903
HinP1I GCGC 2 cut(s) 198, 1903
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 7 cut(s) 170, 269, 1034, 1475, 1553, 1566, 1720
HpaII CCGG 2 cut(s) 27, 1907
HphI GGTGA 4 cut(s) 1406, 1412, 1556, 1957
Hpy166II GTNNAC 5 cut(s) 70, 698, 700, 1292, 1547
Hpy188III TCNNGA 6 cut(s) 167, 1070, 1238, 1511, 1800, 1919
Hpy8I GTNNAC 5 cut(s) 70, 698, 700, 1292, 1547
Hpy99I CGWCG 3 cut(s) 197, 255, 933
HpyAV CCTTC 8 cut(s) 86, 95, 1535, 1589, 1726, 1784, 1819, 1885
HpyCH4III ACNGT 6 cut(s) 177, 360, 614, 884, 1578, 1607
HpyCH4IV ACGT 6 cut(s) 66, 694, 928, 1234, 1349, 1651
HpyCH4V TGCA 6 cut(s) 579, 845, 1025, 1691, 1779, 1934
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 216, 254, 1337, 1697
HpySE526I ACGT 6 cut(s) 66, 694, 928, 1234, 1349, 1651
Hsp92II CATG 7 cut(s) 674, 1127, 1193, 1531, 1553, 1813, 1943
HspAI GCGC 2 cut(s) 198, 1903
KpnI GGTACC 1 cut(s) 1980
KspI CCGCGG 1 cut(s) 943
Kzo9I GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 5 cut(s) 95, 556, 1044, 1078, 1086
Lsp1109I GCAGC 5 cut(s) 400, 829, 1456, 1658, 1671
LweI GCATC 6 cut(s) 500, 748, 854, 1012, 1700, 1819
MaeI CTAG 3 cut(s) 453, 1041, 1505
MaeII ACGT 6 cut(s) 66, 694, 928, 1234, 1349, 1651
MaeIII GTNAC 7 cut(s) 560, 596, 614, 790, 1204, 1230, 1909
MalI GATC 8 cut(s) 276, 306, 426, 913, 967, 1152, 1188, 1804
MbiI CCGCTC 1 cut(s) 134
MboI GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
MflI RGATCY 3 cut(s) 304, 965, 1150
MhlI GDGCHC 5 cut(s) 259, 553, 670, 1083, 1420
MluCI AATT 9 cut(s) 446, 707, 806, 946, 1002, 1091, 1455, 1781, 2000
MlyI GAGTC 4 cut(s) 164, 1028, 1469, 1560
MmeI TCCRAC 1 cut(s) 996
Mph1103I ATGCAT 1 cut(s) 1027
MseI TTAA 6 cut(s) 354, 711, 1094, 1518, 1784, 1887
MslI CAYNNNNRTG 1 cut(s) 584
MspA1I CMGCKG 4 cut(s) 942, 1065, 1451, 1684
MspCI CTTAAG 1 cut(s) 1886
MspI CCGG 2 cut(s) 27, 1907
MspR9I CCNGG 6 cut(s) 309, 1266, 1296, 1708, 1727, 1907
MvaI CCWGG 5 cut(s) 309, 1266, 1296, 1708, 1727
MvnI CGCG 2 cut(s) 222, 942
MwoI GCNNNNNNNGC 5 cut(s) 104, 216, 254, 1337, 1697
NciI CCSGG 1 cut(s) 1907
NdeI CATATG 2 cut(s) 916, 1775
NdeII GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 7 cut(s) 674, 1127, 1193, 1531, 1553, 1813, 1943
NlaIV GGNNCC 9 cut(s) 18, 97, 552, 1107, 1152, 1226, 1418, 1842, 1978
NmeAIII GCCGAG 2 cut(s) 136, 138
NmuCI GTSAC 2 cut(s) 596, 1204
NsiI ATGCAT 1 cut(s) 1027
NspI RCATGY 2 cut(s) 1127, 1531
OliI CACNNNNGTG 1 cut(s) 584
PciI ACATGT 1 cut(s) 1123
PciSI GCTCTTC 1 cut(s) 384
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 165, 201
PfeI GAWTC 3 cut(s) 269, 1553, 1720
PfoI TCCNGGA 2 cut(s) 1264, 1725
PkrI GCNGC 7 cut(s) 109, 221, 390, 844, 1446, 1648, 1686
Ple19I CGATCG 1 cut(s) 277
PleI GAGTC 4 cut(s) 164, 1028, 1469, 1560
PpsI GAGTC 4 cut(s) 164, 1028, 1469, 1560
Ppu21I YACGTR 1 cut(s) 1652
PpuMI RGGWCCY 1 cut(s) 1841
PscI ACATGT 1 cut(s) 1123
Psp124BI GAGCTC 2 cut(s) 670, 1083
Psp1406I AACGTT 1 cut(s) 1349
Psp5II RGGWCCY 1 cut(s) 1841
Psp6I CCWGG 5 cut(s) 307, 1264, 1294, 1706, 1725
PspGI CCWGG 5 cut(s) 307, 1264, 1294, 1706, 1725
PspN4I GGNNCC 9 cut(s) 18, 97, 552, 1107, 1152, 1226, 1418, 1842, 1978
PspOMI GGGCCC 1 cut(s) 1416
PspPI GGNCC 9 cut(s) 247, 686, 1067, 1224, 1289, 1416, 1417, 1841, 1950
PspPPI RGGWCCY 1 cut(s) 1841
PsuI RGATCY 3 cut(s) 304, 965, 1150
PvuI CGATCG 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 1451
RsaI GTAC 5 cut(s) 368, 500, 699, 1796, 1978
RsaNI GTAC 5 cut(s) 367, 499, 698, 1795, 1977
RseI CAYNNNNRTG 1 cut(s) 584
SacI GAGCTC 2 cut(s) 670, 1083
SacII CCGCGG 1 cut(s) 943
SapI GCTCTTC 1 cut(s) 384
SaqAI TTAA 6 cut(s) 354, 711, 1094, 1518, 1784, 1887
SatI GCNGC 7 cut(s) 108, 220, 389, 843, 1445, 1647, 1685
Sau3AI GATC 8 cut(s) 274, 304, 424, 911, 965, 1150, 1186, 1802
Sau96I GGNCC 9 cut(s) 247, 686, 1067, 1224, 1289, 1416, 1417, 1841, 1950
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 4 cut(s) 164, 1028, 1469, 1560
ScrFI CCNGG 6 cut(s) 309, 1266, 1296, 1708, 1727, 1907
SduI GDGCHC 5 cut(s) 259, 553, 670, 1083, 1420
SfaNI GCATC 6 cut(s) 500, 748, 854, 1012, 1700, 1819
Sfr303I CCGCGG 1 cut(s) 943
SgrBI CCGCGG 1 cut(s) 943
SinI GGWCC 3 cut(s) 1067, 1289, 1841
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 1886
SmoI CTYRAG 1 cut(s) 1886
Sse9I AATT 9 cut(s) 446, 707, 806, 946, 1002, 1091, 1455, 1781, 2000
SspMI CTAG 3 cut(s) 453, 1041, 1505
SstI GAGCTC 2 cut(s) 670, 1083
StyD4I CCNGG 6 cut(s) 307, 1264, 1294, 1706, 1725, 1905
TaaI ACNGT 6 cut(s) 177, 360, 614, 884, 1578, 1607
TaiI ACGT 6 cut(s) 69, 697, 931, 1237, 1352, 1654
TaqI TCGA 7 cut(s) 168, 192, 288, 931, 1162, 1473, 1624
TaqII GACCGA 1 cut(s) 1277
TasI AATT 9 cut(s) 446, 707, 806, 946, 1002, 1091, 1455, 1781, 2000
TatI WGTACW 3 cut(s) 366, 498, 697
TauI GCSGC 2 cut(s) 110, 222
TfiI GAWTC 3 cut(s) 269, 1553, 1720
Tru1I TTAA 6 cut(s) 354, 711, 1094, 1518, 1784, 1887
Tru9I TTAA 6 cut(s) 354, 711, 1094, 1518, 1784, 1887
TscAI CASTG 2 cut(s) 889, 1612
TseFI GTSAC 2 cut(s) 596, 1204
TseI GCWGC 5 cut(s) 388, 842, 1444, 1646, 1684
Tsp45I GTSAC 2 cut(s) 596, 1204
TspDTI ATGAA 7 cut(s) 749, 768, 1362, 1482, 1566, 1656, 1826
TspGWI ACGGA 1 cut(s) 1223
TspRI CASTG 2 cut(s) 889, 1612
Vha464I CTTAAG 1 cut(s) 1886
VpaK11BI GGWCC 3 cut(s) 1067, 1289, 1841
XagI CCTNNNNNAGG 1 cut(s) 1730
XapI RAATTY 1 cut(s) 707
XceI RCATGY 2 cut(s) 1127, 1531
XcmI CCANNNNNNNNNTGG 3 cut(s) 805, 811, 1161
XspI CTAG 3 cut(s) 453, 1041, 1505
ZrmI AGTACT 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.