pycom17g00970

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012914
Physical Location & Seq
Reverse (-)
702091 .. 706253
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g00970.5

Sequence Viewer

Length: 2064 bp
ATGTTGACCAAAGTAAGAGGAAAAGGGAAAATGGCGGGAAAGGAGGAAGCGAAGGGGCGGCAAGGAGCGACCGGCGGCGAACAGGTCTGCTGGAGAAAGCAAGTGGACGATAACCTAAACAGGCTCCATTCTCTTTCCTTCGGAGCCGAGCTCGCCCTCGAGAGGCACGACTTCTCCTCCGCTCAGATCCTCGGCCTTCGTCTCCTCGGCTTCCTCGACTCCCAATCTCACTCTCCGCTCGACCACGCCTTCATCCGCCCCATCCGCCGCGAAACCGTCGCCAAACTCCACGCTGCCCGCCGTGCCCTCATTCCTCTTTCCGATCGCAAAGTCTTTGAGCAAGCAAAGAAGGCGCCCGGCCATGTTTTCAGCACAAATGGCGATATTGACATTGAGAAGATTAAGCAGTCAAAGTACTTTCAGGCTCTTCTTCAGCAGTCTAATAACGGAAGGCCTGCTAATGATATGGGAGATCGATTGGAGAGGCAGGACAAGTTGACTAGCAAGTTATCGAAACCTATGGCACAAACAAAGTTGACATCGTTGTACAAACACAACTCGATAGTGGAAAGTAATGGCAATTCCAAGGATTCTTGGGCCTGTAGAAGTAACAGTTCCGAGGACTGCATTGTTGTAGAAAAGCTTCACACTTACGAAAACCATAATGGCAATCTTAGCGCCTCATCTTTTCAAAATGTTGAAGAGGAAGGAACTTATGGGAATACCTTTAGGGCTAAACGTGCACACAAGGAATTTGCTAGCCCCATCATTGATACAGCAAAGTCACCATCAGCCAACGAAGATGCTAAAGCTGATGGTTCTGGAAATAGCTTTGTTACTGCTAAAGCCAAATTGGAAATGGATGTGAGGCAAAGAAGAGGATTAGCGCCAGTGGGATCACCAAATTCTTGTATCTCCCCTCAAAGCGATAACAATGCTGCTAATAGGGGTTATGGTATGAGATCATATGGCTATTTACGTCGCGGTGCCCGTGGTAATTTTGTTCCCCCTATTAAATCTAATGAGGGAAATGTTGGGAATGTGACTTCACAAGTCGCTGGAAAGTCAGATGATGCATTAGGTGACTCAACTAAGAGATGTTTGGAATTGCTCTGTGGTCCTGATGGAGAGCTCCCAGAAAAATTAAGGAACTTGGAACCTCGTCTTCTTGAACATGTTAGTAATGAGATTATGGACAAGGATCCGAATGTTCGATGGAATGATATTGCTGGTTTGGAGCATGCTAAGAAATGTGTAACCGAGATGGTCATATGGCCTCTACTACGTCCTGACATTTTTAAAGGTTGCCGTTCCCCTGGGAAAGGCCTTCTTCTCTTTGGTCCACCAGGAACTGGAAAAACAATGATCGGGAAAGCCATAGCTGGAGAAGCAAAAGCAACCTTTTTCTACATATCTGCCAGTTCATTAACAAGCAAATGGATTGGCGAGGGCGAAAAGCTAGTGCGAGCACTGTTTGGAGTTGCAAGTTGTCGTCAGCCTGCTGTAATTTTTGTTGATGAAATTGATTCACTTCTCTCACAGCGTAAGTCAGAAGGTGAGCATGAATCAAGTAGACGACTCAAAACACAGTTCCTTATTGAGATGGAAGGCTTTGACAGTGGCAGTGAGCAAATTCTTCTTATAGGAGCAACGAATCGACCCCAAGAACTTGATGAAGCAGCGAGGAGGCGACTTACCAAGAGGCTTTACATTCCCCTGCCTTCATCAGAAGCAAGATCGATCGATAGTTGGATCATACGGAATCTCTTAGAAAAAGATGGGCTGTTCAAACTTTCAAGGGAGGACATTGATAGCATATGCAATTTGACCGAAGGGTACTCAGGATCAGACATGAAAAACTTGGTGAAGGATGCCTCAATGGGTCCACTAAGAGAAGCTCTGAAACAAGGCATCGAAATAACAAAGCTGAAAAAAGAGGATATGCGTCCGGTAACTGTTCAGGACTTTGAGAGTGCATTGCAAGAAGTGAGGCCTTCTGTTTCGGTGAACGAGCTTGGCACGTATGAGGAATGGAACAAACAATTTGGAAGCTTGTCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000228 GO:0000280 GO:0000287 GO:0001503 GO:0001649 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006163 GO:0006282 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007140 GO:0007276 GO:0008150 GO:0008152 GO:0008283 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009314 GO:0009628 GO:0009892 GO:0009987 GO:0010212 GO:0010520 GO:0010564 GO:0010569 GO:0010605 GO:0010639 GO:0010941 GO:0010948 GO:0016043 GO:0016787 GO:0017144 GO:0019219 GO:0019222 GO:0019637 GO:0019693 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0033687 GO:0034641 GO:0040020 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045128 GO:0045786 GO:0045835 GO:0045910 GO:0045934 GO:0046034 GO:0046483 GO:0046872 GO:0048232 GO:0048285 GO:0048471 GO:0048519 GO:0048523 GO:0048583 GO:0048609 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051321 GO:0051445 GO:0051447 GO:0051704 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0055086 GO:0060255 GO:0060548 GO:0060631 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0071840 GO:0072521 GO:0080090 GO:0080134 GO:0080135 GO:0104004 GO:0110029 GO:0140013 GO:1901135 GO:1901360 GO:1901564 GO:1903046 GO:2000241 GO:2000242 GO:2000779 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Vps4_C

Protein Analysis

688

Amino Acids

76.02

Weight (kDa)

8.9

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012914)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27120
fragaria_vesca FvH4_6g53320
malus_domestica MD09G1011500.v1.1 MD17G1012000.v1.1
prunus_persica Prupe.3G305500_v2.0.a1
pyrus_communis pycom111g00980 pycom17g00970
rosa_chinensis RchiOBHm_Chr2g0175481
rosa_laevigata RLG00000022331
rosa_multiflora Rmu_sc0003302.1_g000002 Rmu_sc0021955.1_g000001
rosa_roxburghii Rroxscaffold_2G00077280
rosa_rugosa Rorug02G0585100
rosa_samantha Rh2AG664600 Rh2BG675300 Rh2CG638500 Rh2DG689300
rosa_wichuraiana Rw2G054470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 352, 986
AccB7I CCANNNNNTGG 1 cut(s) 1352
AccBSI CCGCTC 2 cut(s) 182, 238
AccI GTMKAC 1 cut(s) 1573
AccII CGCG 2 cut(s) 270, 984
AclWI GGATC 6 cut(s) 181, 904, 1196, 1209, 1760, 1852
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 3 cut(s) 752, 904, 1632
AcuI CTGAAG 1 cut(s) 416
AcyI GRCGYC 1 cut(s) 353
AfaI GTAC 3 cut(s) 416, 548, 1838
AfiI CCNNNNNNNGG 3 cut(s) 162, 1322, 1352
AflIII ACRYGT 1 cut(s) 1174
AgsI TTSAA 5 cut(s) 692, 701, 1172, 1789, 1797
AjnI CCWGG 2 cut(s) 1315, 1345
Alw21I GWGCWC 4 cut(s) 153, 745, 1134, 1471
Alw26I GTCTC 1 cut(s) 206
Alw44I GTGCAC 1 cut(s) 741
AlwI GGATC 6 cut(s) 181, 904, 1196, 1209, 1760, 1852
AlwNI CAGNNNCTG 1 cut(s) 1352
Ama87I CYCGRG 1 cut(s) 158
AoxI GGCC 7 cut(s) 193, 358, 452, 597, 1274, 1324, 1991
ApaLI GTGCAC 1 cut(s) 741
ApeKI GCWGC 3 cut(s) 293, 938, 1679
ApoI RAATTY 3 cut(s) 752, 904, 1632
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 3 cut(s) 355, 680, 889
AspS9I GGNCC 4 cut(s) 597, 1118, 1340, 1883
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 6 cut(s) 777, 891, 1094, 1568, 1876, 2017
AsuNHI GCTAGC 1 cut(s) 758
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 3 cut(s) 1118, 1340, 1883
BaeGI GKGCMC 3 cut(s) 307, 745, 991
BamHI GGATCC 1 cut(s) 1201
BanI GGYRCC 2 cut(s) 352, 986
BanII GRGCYC 2 cut(s) 153, 1134
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 4 cut(s) 153, 745, 1134, 1471
BbvI GCAGC 3 cut(s) 280, 925, 1691
BccI CCATC 9 cut(s) 269, 773, 796, 809, 1118, 1209, 1258, 1597, 1772
BceAI ACGGC 2 cut(s) 285, 1293
BcgI CGANNNNNNTGC 2 cut(s) 917, 951
BciT130I CCWGG 2 cut(s) 1317, 1347
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 3 cut(s) 501, 759, 1460
BfmI CTRYAG 1 cut(s) 601
BfoI RGCGCY 3 cut(s) 356, 681, 890
BisI GCNGC 6 cut(s) 59, 76, 268, 294, 939, 1680
BlsI GCNGC 6 cut(s) 60, 77, 269, 295, 940, 1681
BmcAI AGTACT 1 cut(s) 416
Bme1390I CCNGG 3 cut(s) 357, 1317, 1347
Bme18I GGWCC 3 cut(s) 1118, 1340, 1883
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 4 cut(s) 597, 1118, 1340, 1883
BmiI GGNNCC 7 cut(s) 125, 145, 354, 988, 1158, 1203, 1884
BmrFI CCNGG 3 cut(s) 357, 1317, 1347
BmsI GCATC 4 cut(s) 793, 1063, 1861, 1920
BmtI GCTAGC 1 cut(s) 762
BpiI GAAGAC 1 cut(s) 1157
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 2 cut(s) 112, 1404
BpuMI CCSGG 1 cut(s) 357
Bsa29I ATCGAT 3 cut(s) 475, 1739, 1743
BsaAI YACGTR 1 cut(s) 2022
BsaHI GRCGYC 1 cut(s) 353
BsaJI CCNNGG 7 cut(s) 190, 205, 585, 618, 991, 1315, 1316
BsaWI WCCGGW 1 cut(s) 1948
BsaXI ACNNNNNCTCC 4 cut(s) 158, 161, 188, 191
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 1322, 1352
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 890, 1357, 1419
Bse3DI GCAATG 1 cut(s) 1976
BseBI CCWGG 2 cut(s) 1317, 1347
BseCI ATCGAT 3 cut(s) 475, 1739, 1743
BseDI CCNNGG 7 cut(s) 190, 205, 585, 618, 991, 1315, 1316
BseGI GGATG 4 cut(s) 252, 261, 868, 1876
BseLI CCNNNNNNNGG 3 cut(s) 162, 1322, 1352
BseMI GCAATG 1 cut(s) 1976
BseMII CTCAG 2 cut(s) 197, 1854
BseNI ACTGG 3 cut(s) 890, 1357, 1419
BseRI GAGGAG 3 cut(s) 166, 194, 1699
BseSI GKGCMC 3 cut(s) 307, 745, 991
BseXI GCAGC 3 cut(s) 280, 925, 1691
Bsh1236I CGCG 2 cut(s) 270, 984
Bsh1285I CGRYCG 3 cut(s) 72, 325, 1743
BshFI GGCC 7 cut(s) 195, 360, 454, 599, 1276, 1326, 1993
BshNI GGYRCC 2 cut(s) 352, 986
BshVI ATCGAT 3 cut(s) 475, 1739, 1743
BsiEI CGRYCG 3 cut(s) 72, 325, 1743
BsiHKAI GWGCWC 4 cut(s) 153, 745, 1134, 1471
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 3 cut(s) 72, 357, 1949
BslI CCNNNNNNNGG 3 cut(s) 162, 1322, 1352
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsnI GGCC 7 cut(s) 195, 360, 454, 599, 1276, 1326, 1993
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 6 cut(s) 153, 307, 745, 991, 1134, 1471
Bsp1407I TGTACA 1 cut(s) 546
BspANI GGCC 7 cut(s) 195, 360, 454, 599, 1276, 1326, 1993
BspCNI CTCAG 2 cut(s) 196, 1853
BspDI ATCGAT 3 cut(s) 475, 1739, 1743
BspFNI CGCG 2 cut(s) 270, 984
BspLI GGNNCC 7 cut(s) 125, 145, 354, 988, 1158, 1203, 1884
BspOI GCTAGC 1 cut(s) 762
BspPI GGATC 6 cut(s) 181, 904, 1196, 1209, 1760, 1852
BspQI GCTCTTC 1 cut(s) 432
BspT107I GGYRCC 2 cut(s) 352, 986
BsrBI CCGCTC 2 cut(s) 182, 238
BsrDI GCAATG 1 cut(s) 1976
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 3 cut(s) 890, 1357, 1419
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 7 cut(s) 190, 205, 585, 618, 991, 1315, 1316
BssNI GRCGYC 1 cut(s) 353
BssT1I CCWWGG 1 cut(s) 585
Bst2UI CCWGG 2 cut(s) 1317, 1347
Bst4CI ACNGT 7 cut(s) 277, 614, 1473, 1590, 1619, 1957, 2061
Bst6I CTCTTC 3 cut(s) 432, 696, 871
BstACI GRCGYC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 546
BstBAI YACGTR 1 cut(s) 2022
BstC8I GCNNGC 8 cut(s) 153, 298, 342, 456, 760, 1242, 1467, 1500
BstDEI CTNAG 7 cut(s) 183, 674, 1092, 1245, 1768, 1840, 1889
BstDSI CCRYGG 1 cut(s) 991
BstENI CCTNNNNNAGG 1 cut(s) 1320
BstF5I GGATG 4 cut(s) 252, 261, 868, 1876
BstFNI CGCG 2 cut(s) 270, 984
BstH2I RGCGCY 3 cut(s) 356, 681, 890
BstHHI GCGC 3 cut(s) 355, 680, 889
BstMAI GTCTC 1 cut(s) 206
BstMCI CGRYCG 3 cut(s) 72, 325, 1743
BstMWI GCNNNNNNNGC 8 cut(s) 152, 264, 302, 350, 378, 675, 740, 1388
BstNI CCWGG 2 cut(s) 1317, 1347
BstNSI RCATGY 2 cut(s) 1178, 1244
BstSCI CCNGG 3 cut(s) 355, 1315, 1345
BstSFI CTRYAG 1 cut(s) 601
BstSLI GKGCMC 3 cut(s) 307, 745, 991
BstUI CGCG 2 cut(s) 270, 984
BstV1I GCAGC 3 cut(s) 280, 925, 1691
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 2 cut(s) 186, 1201
BstYI RGATCY 2 cut(s) 186, 1201
Bsu15I ATCGAT 3 cut(s) 475, 1739, 1743
BsuRI GGCC 7 cut(s) 195, 360, 454, 599, 1276, 1326, 1993
BsuTUI ATCGAT 3 cut(s) 475, 1739, 1743
BtgI CCRYGG 1 cut(s) 991
BtsCI GGATG 4 cut(s) 252, 261, 868, 1876
BtsI GCAGTG 1 cut(s) 1630
BtsIMutI CAGTG 5 cut(s) 897, 1469, 1624, 1630, 2057
Cac8I GCNNGC 8 cut(s) 153, 298, 342, 456, 760, 1242, 1467, 1500
CaiI CAGNNNCTG 1 cut(s) 1352
CfoI GCGC 3 cut(s) 355, 680, 889
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 4 cut(s) 597, 1118, 1340, 1883
ClaI ATCGAT 3 cut(s) 475, 1739, 1743
CseI GACGC 1 cut(s) 1934
Csp6I GTAC 3 cut(s) 415, 547, 1837
CviAII CATG 5 cut(s) 362, 1175, 1241, 1562, 1852
CviQI GTAC 3 cut(s) 415, 547, 1837
DdeI CTNAG 7 cut(s) 183, 674, 1092, 1245, 1768, 1840, 1889
DinI GGCGCC 1 cut(s) 354
DraI TTTAAA 1 cut(s) 1300
EaeI YGGCCR 1 cut(s) 358
Eam1104I CTCTTC 3 cut(s) 432, 696, 871
EarI CTCTTC 3 cut(s) 432, 696, 871
EciI GGCGGA 2 cut(s) 245, 254
Ecl136II GAGCTC 2 cut(s) 151, 1132
Eco130I CCWWGG 1 cut(s) 585
Eco147I AGGCCT 3 cut(s) 454, 1326, 1993
Eco24I GRGCYC 2 cut(s) 153, 1134
Eco47I GGWCC 3 cut(s) 1118, 1340, 1883
Eco53kI GAGCTC 2 cut(s) 151, 1132
Eco57I CTGAAG 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 2 cut(s) 151, 1132
EcoNI CCTNNNNNAGG 1 cut(s) 1320
EcoRII CCWGG 2 cut(s) 1315, 1345
EcoT14I CCWWGG 1 cut(s) 585
EcoT22I ATGCAT 1 cut(s) 1078
EcoT38I GRGCYC 2 cut(s) 153, 1134
EgeI GGCGCC 1 cut(s) 354
EheI GGCGCC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 5 cut(s) 365, 1178, 1244, 1565, 1855
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FatI CATG 5 cut(s) 361, 1174, 1240, 1561, 1851
FauI CCCGC 2 cut(s) 28, 305
FauNDI CATATG 3 cut(s) 967, 1271, 1817
FblI GTMKAC 1 cut(s) 1573
Fnu4HI GCNGC 6 cut(s) 59, 76, 268, 294, 939, 1680
FokI GGATG 4 cut(s) 239, 248, 875, 1883
FriOI GRGCYC 2 cut(s) 153, 1134
Fsp4HI GCNGC 6 cut(s) 59, 76, 268, 294, 939, 1680
FspBI CTAG 3 cut(s) 501, 759, 1460
GlaI GCGC 3 cut(s) 354, 679, 888
GluI GCNGC 6 cut(s) 59, 76, 268, 294, 939, 1680
GsuI CTGGAG 2 cut(s) 112, 1404
HaeII RGCGCY 3 cut(s) 356, 681, 890
HaeIII GGCC 7 cut(s) 195, 360, 454, 599, 1276, 1326, 1993
HapII CCGG 3 cut(s) 72, 357, 1949
HgaI GACGC 1 cut(s) 1934
HhaI GCGC 3 cut(s) 355, 680, 889
Hin1I GRCGYC 1 cut(s) 353
Hin1II CATG 5 cut(s) 365, 1178, 1244, 1565, 1855
Hin6I GCGC 3 cut(s) 353, 678, 887
HinP1I GCGC 3 cut(s) 353, 678, 887
HincII GTYRAC 3 cut(s) 6, 498, 537
HindII GTYRAC 3 cut(s) 6, 498, 537
HindIII AAGCTT 2 cut(s) 641, 2050
HinfI GANTC 8 cut(s) 218, 590, 1085, 1526, 1565, 1578, 1654, 1762
HpaII CCGG 3 cut(s) 72, 357, 1949
HphI GGTGA 6 cut(s) 777, 891, 1094, 1568, 1876, 2017
Hpy166II GTNNAC 9 cut(s) 6, 106, 498, 537, 743, 1343, 1574, 1886, 2008
Hpy188III TCNNGA 8 cut(s) 160, 822, 1121, 1169, 1289, 1369, 1842, 1961
Hpy8I GTNNAC 9 cut(s) 6, 106, 498, 537, 743, 1343, 1574, 1886, 2008
Hpy99I CGWCG 2 cut(s) 281, 984
HpyCH4III ACNGT 7 cut(s) 277, 614, 1473, 1590, 1619, 1957, 2061
HpyCH4IV ACGT 4 cut(s) 739, 979, 1285, 2021
HpyCH4V TGCA 7 cut(s) 627, 743, 1076, 1484, 1821, 1976, 1981
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 264, 302, 350, 378, 675, 740, 1388
HpyF3I CTNAG 7 cut(s) 183, 674, 1092, 1245, 1768, 1840, 1889
HpySE526I ACGT 4 cut(s) 739, 979, 1285, 2021
Hsp92I GRCGYC 1 cut(s) 353
Hsp92II CATG 5 cut(s) 365, 1178, 1244, 1565, 1855
HspAI GCGC 3 cut(s) 353, 678, 887
KasI GGCGCC 1 cut(s) 352
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 6 cut(s) 65, 129, 143, 1137, 1237, 1646
Lsp1109I GCAGC 3 cut(s) 280, 925, 1691
LweI GCATC 4 cut(s) 793, 1063, 1861, 1920
MaeI CTAG 3 cut(s) 501, 759, 1460
MaeII ACGT 4 cut(s) 739, 979, 1285, 2021
MaeIII GTNAC 8 cut(s) 608, 783, 835, 1042, 1082, 1255, 1951, 2055
MbiI CCGCTC 2 cut(s) 182, 238
MboII GAAGA 9 cut(s) 409, 419, 422, 713, 812, 888, 1157, 1322, 1628
MflI RGATCY 2 cut(s) 186, 1201
MhlI GDGCHC 6 cut(s) 153, 307, 745, 991, 1134, 1471
Mly113I GGCGCC 1 cut(s) 353
MlyI GAGTC 3 cut(s) 212, 1079, 1572
MmeI TCCRAC 1 cut(s) 1730
Mph1103I ATGCAT 1 cut(s) 1078
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 5 cut(s) 402, 1014, 1145, 1299, 1427
MspI CCGG 3 cut(s) 72, 357, 1949
MspR9I CCNGG 3 cut(s) 357, 1317, 1347
MvaI CCWGG 2 cut(s) 1317, 1347
MvnI CGCG 2 cut(s) 270, 984
MwoI GCNNNNNNNGC 8 cut(s) 152, 264, 302, 350, 378, 675, 740, 1388
NarI GGCGCC 1 cut(s) 353
NciI CCSGG 1 cut(s) 357
NdeI CATATG 3 cut(s) 967, 1271, 1817
NheI GCTAGC 1 cut(s) 758
NlaIII CATG 5 cut(s) 365, 1178, 1244, 1565, 1855
NlaIV GGNNCC 7 cut(s) 125, 145, 354, 988, 1158, 1203, 1884
NmeAIII GCCGAG 3 cut(s) 171, 172, 186
NmuCI GTSAC 4 cut(s) 783, 1042, 1082, 2055
NsiI ATGCAT 1 cut(s) 1078
NspI RCATGY 2 cut(s) 1178, 1244
PaeI GCATGC 1 cut(s) 1244
PaeR7I CTCGAG 1 cut(s) 158
PasI CCCWGGG 1 cut(s) 1316
PceI AGGCCT 3 cut(s) 454, 1326, 1993
PciI ACATGT 1 cut(s) 1174
PciSI GCTCTTC 1 cut(s) 432
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 213, 988
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 5 cut(s) 590, 1526, 1565, 1654, 1762
PflMI CCANNNNNTGG 1 cut(s) 1352
PkrI GCNGC 6 cut(s) 60, 77, 269, 295, 940, 1681
Ple19I CGATCG 2 cut(s) 325, 1743
PleI GAGTC 3 cut(s) 212, 1079, 1572
PluTI GGCGCC 1 cut(s) 356
PpsI GAGTC 3 cut(s) 212, 1079, 1572
Ppu21I YACGTR 1 cut(s) 2022
PscI ACATGT 1 cut(s) 1174
Psp124BI GAGCTC 2 cut(s) 153, 1134
Psp6I CCWGG 2 cut(s) 1315, 1345
PspGI CCWGG 2 cut(s) 1315, 1345
PspN4I GGNNCC 7 cut(s) 125, 145, 354, 988, 1158, 1203, 1884
PspPI GGNCC 4 cut(s) 597, 1118, 1340, 1883
PstNI CAGNNNCTG 1 cut(s) 1352
PsuI RGATCY 2 cut(s) 186, 1201
PvuI CGATCG 2 cut(s) 325, 1743
RsaI GTAC 3 cut(s) 416, 548, 1838
RsaNI GTAC 3 cut(s) 415, 547, 1837
SacI GAGCTC 2 cut(s) 153, 1134
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 5 cut(s) 402, 1014, 1145, 1299, 1427
SatI GCNGC 6 cut(s) 59, 76, 268, 294, 939, 1680
Sau96I GGNCC 4 cut(s) 597, 1118, 1340, 1883
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 3 cut(s) 212, 1079, 1572
ScrFI CCNGG 3 cut(s) 357, 1317, 1347
SduI GDGCHC 6 cut(s) 153, 307, 745, 991, 1134, 1471
SfaNI GCATC 4 cut(s) 793, 1063, 1861, 1920
SfcI CTRYAG 1 cut(s) 601
SfoI GGCGCC 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 158
SinI GGWCC 3 cut(s) 1118, 1340, 1883
SlaI CTCGAG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SphI GCATGC 1 cut(s) 1244
SseBI AGGCCT 3 cut(s) 454, 1326, 1993
SspDI GGCGCC 1 cut(s) 352
SspMI CTAG 3 cut(s) 501, 759, 1460
SstI GAGCTC 2 cut(s) 153, 1134
StuI AGGCCT 3 cut(s) 454, 1326, 1993
StyD4I CCNGG 3 cut(s) 355, 1315, 1345
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 7 cut(s) 277, 614, 1473, 1590, 1619, 1957, 2061
TaiI ACGT 4 cut(s) 742, 982, 1288, 2024
TaqII GACCGA 1 cut(s) 1844
TatI WGTACW 2 cut(s) 414, 546
TauI GCSGC 3 cut(s) 61, 78, 270
TfiI GAWTC 5 cut(s) 590, 1526, 1565, 1654, 1762
Tru1I TTAA 5 cut(s) 402, 1014, 1145, 1299, 1427
Tru9I TTAA 5 cut(s) 402, 1014, 1145, 1299, 1427
TscAI CASTG 5 cut(s) 897, 1476, 1624, 1630, 2064
TseFI GTSAC 4 cut(s) 783, 1042, 1082, 2055
TseI GCWGC 3 cut(s) 293, 938, 1679
Tsp45I GTSAC 4 cut(s) 783, 1042, 1082, 2055
TspDTI ATGAA 7 cut(s) 241, 1413, 1533, 1578, 1689, 1713, 1868
TspGWI ACGGA 2 cut(s) 462, 1774
TspRI CASTG 5 cut(s) 897, 1476, 1624, 1630, 2064
Van91I CCANNNNNTGG 1 cut(s) 1352
VneI GTGCAC 1 cut(s) 741
VpaK11BI GGWCC 3 cut(s) 1118, 1340, 1883
XagI CCTNNNNNAGG 1 cut(s) 1320
XapI RAATTY 3 cut(s) 752, 904, 1632
XceI RCATGY 2 cut(s) 1178, 1244
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XhoI CTCGAG 1 cut(s) 158
XmiI GTMKAC 1 cut(s) 1573
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 3 cut(s) 501, 759, 1460
ZrmI AGTACT 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.