MD09G1018600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014170
Physical Location & Seq
Reverse (-)
1151487 .. 1152080
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1018600.v1.1.491

Sequence Viewer

Length: 594 bp
ATGAAGAGAGAGGGTCGCCAGCATGGGATGGTCCGGACACACCGGATCCTACCGGAGCCGTTGAACCCGAGACCGGCAACCCGACACGTCAACAAGTTCGAGTCTCCGCCCACGGCCGGATTGTTCGCCAAGGTGCCAACCAAGCCCACCAACCACTCCAAGTTCACCGGCAAGTGCGGCAAGCCCAAGTGCACCGAGTGTCACATTCACCCGGCTTCCAAGGCCAAGGACAAGACCAAGGGCAACCGAAAGCTCAAGTCTTCCGACGTCGTTTCCAATCATCGGTTGGTCACTTGGCGGGTCGTGGACGGGCAACCCGGCTTGAATAGTCAATTGGGTTTGTCCGCCACGGGGTTGTTGGATCGTCTCTCTAGGGATTATGAGAATGACTATGATGATGGTGACGATGATCATGAAATCAATGGTTATGCAGTTGGATATTATGATAGAGTTGATGATCGGACGGTGGAAAATAATGTTGATGATGTTGAGCATCATGGTGATTACGAAGAGATGGGTTTCTTTAATGTGGATTATGTGATTGATCATCAAATCGAGGAAGATGATGGTTGGTGCTTAGTGCCACAAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.35

Weight (kDa)

5.98

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40690
fragaria_vesca FvH4_6g52330
malus_domestica MD09G1018600.v1.1 MD17G1019100.v1.1
prunus_persica Prupe.3G298500_v2.0.a1 Prupe.3G298600_v2.0.a1
pyrus_communis pycom111g01550 pycom17g01590
rosa_chinensis RchiOBHm_Chr2g0173921
rosa_laevigata RLG00000022234
rosa_multiflora Rmu_co8413491.1_g000001
rosa_rugosa Rorug02G0573800
rosa_samantha Rh2AG652700 Rh2BG663900 Rh2CG628500 Rh2DG678800
rosa_wichuraiana Rw2G053620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 133
AccIII TCCGGA 1 cut(s) 33
AciI CCGC 4 cut(s) 107, 177, 298, 345
AclWI GGATC 3 cut(s) 40, 53, 369
AcoI YGGCCR 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 198
AfiI CCNNNNNNNGG 4 cut(s) 73, 116, 282, 351
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 2 cut(s) 64, 325
AjiI CACGTC 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AleI CACNNNNGTG 1 cut(s) 589
AluBI AGCT 1 cut(s) 253
AluI AGCT 1 cut(s) 253
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 3 cut(s) 64, 108, 371
Alw44I GTGCAC 1 cut(s) 190
AlwI GGATC 3 cut(s) 40, 53, 369
Ama87I CYCGRG 1 cut(s) 67
Aor13HI TCCGGA 1 cut(s) 33
AoxI GGCC 2 cut(s) 114, 222
ApaLI GTGCAC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 31
AsuC2I CCSGG 2 cut(s) 212, 318
AsuHPI GGTGA 4 cut(s) 157, 200, 413, 512
AvaI CYCGRG 1 cut(s) 67
AvaII GGWCC 1 cut(s) 31
BaeGI GKGCMC 1 cut(s) 194
BamHI GGATCC 1 cut(s) 45
BanI GGYRCC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 194
BccI CCATC 4 cut(s) 22, 392, 508, 560
BceAI ACGGC 2 cut(s) 43, 129
BclI TGATCA 2 cut(s) 409, 544
BcnI CCSGG 2 cut(s) 212, 318
BcoDI GTCTC 3 cut(s) 64, 108, 371
BfaI CTAG 1 cut(s) 372
BisI GCNGC 1 cut(s) 178
BlsI GCNGC 1 cut(s) 179
Bme1390I CCNGG 2 cut(s) 212, 318
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 67
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 3 cut(s) 47, 57, 135
BmrFI CCNGG 2 cut(s) 212, 318
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 212, 318
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 6 cut(s) 111, 129, 219, 225, 237, 348
BsaWI WCCGGW 3 cut(s) 33, 42, 52
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 116, 282, 351
Bse118I RCCGGY 2 cut(s) 73, 167
BseAI TCCGGA 1 cut(s) 33
BseDI CCNNGG 6 cut(s) 111, 129, 219, 225, 237, 348
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 4 cut(s) 73, 116, 282, 351
BseSI GKGCMC 1 cut(s) 194
BseX3I CGGCCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 2 cut(s) 116, 224
BshNI GGYRCC 1 cut(s) 133
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 67
BsiSI CCGG 8 cut(s) 34, 43, 53, 74, 117, 168, 212, 318
BslI CCNNNNNNNGG 4 cut(s) 73, 116, 282, 351
BsmAI GTCTC 3 cut(s) 64, 108, 371
BsmBI CGTCTC 1 cut(s) 371
BsnI GGCC 2 cut(s) 116, 224
Bso31I GGTCTC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 194
Bsp13I TCCGGA 1 cut(s) 33
Bsp143I GATC 5 cut(s) 45, 361, 409, 457, 544
BspACI CCGC 4 cut(s) 107, 177, 298, 345
BspANI GGCC 2 cut(s) 116, 224
BspEI TCCGGA 1 cut(s) 33
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 47, 57, 135
BspPI GGATC 3 cut(s) 40, 53, 369
BspT107I GGYRCC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 64
BsrFI RCCGGY 2 cut(s) 73, 167
BssAI RCCGGY 2 cut(s) 73, 167
BssECI CCNNGG 6 cut(s) 111, 129, 219, 225, 237, 348
BssMI GATC 5 cut(s) 45, 361, 409, 457, 544
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 4 cut(s) 129, 219, 225, 237
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 504
BstACI GRCGYC 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 20, 182
BstDEI CTNAG 1 cut(s) 577
BstDSI CCRYGG 2 cut(s) 111, 348
BstF5I GGATG 1 cut(s) 33
BstKTI GATC 5 cut(s) 48, 364, 412, 460, 547
BstMAI GTCTC 3 cut(s) 64, 108, 371
BstMBI GATC 5 cut(s) 45, 361, 409, 457, 544
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 3 cut(s) 142, 177, 221
BstSCI CCNGG 2 cut(s) 210, 316
BstSLI GKGCMC 1 cut(s) 194
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BstZI CGGCCG 1 cut(s) 114
BsuRI GGCC 2 cut(s) 116, 224
BtgI CCRYGG 2 cut(s) 111, 348
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 1 cut(s) 33
Cac8I GCNNGC 2 cut(s) 20, 182
CciI TCATGA 1 cut(s) 412
Cfr10I RCCGGY 2 cut(s) 73, 167
Cfr13I GGNCC 1 cut(s) 31
CviAII CATG 3 cut(s) 23, 413, 497
CviJI RGCY 8 cut(s) 58, 116, 145, 184, 215, 224, 253, 321
CviKI_1 RGCY 8 cut(s) 58, 116, 145, 184, 215, 224, 253, 321
DdeI CTNAG 1 cut(s) 577
DpnI GATC 5 cut(s) 47, 363, 411, 459, 546
DpnII GATC 5 cut(s) 45, 361, 409, 457, 544
DraIII CACNNNGTG 1 cut(s) 198
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
EciI GGCGGA 2 cut(s) 96, 334
EclXI CGGCCG 1 cut(s) 114
Eco130I CCWWGG 4 cut(s) 129, 219, 225, 237
Eco31I GGTCTC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 31
Eco52I CGGCCG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 67
EcoT14I CCWWGG 4 cut(s) 129, 219, 225, 237
ErhI CCWWGG 4 cut(s) 129, 219, 225, 237
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 3 cut(s) 26, 416, 500
FaiI YATR 8 cut(s) 24, 381, 393, 414, 429, 444, 498, 537
FatI CATG 3 cut(s) 22, 412, 496
FauI CCCGC 1 cut(s) 291
FbaI TGATCA 2 cut(s) 409, 544
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 1 cut(s) 372
GluI GCNGC 1 cut(s) 178
HaeIII GGCC 2 cut(s) 116, 224
HapII CCGG 8 cut(s) 34, 43, 53, 74, 117, 168, 212, 318
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 26, 416, 500
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 1 cut(s) 101
HpaII CCGG 8 cut(s) 34, 43, 53, 74, 117, 168, 212, 318
HphI GGTGA 4 cut(s) 157, 200, 413, 512
Hpy166II GTNNAC 4 cut(s) 91, 165, 192, 307
Hpy188I TCNGA 2 cut(s) 265, 462
Hpy188III TCNNGA 2 cut(s) 34, 413
Hpy8I GTNNAC 4 cut(s) 91, 165, 192, 307
Hpy99I CGWCG 2 cut(s) 269, 272
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 3 cut(s) 87, 267, 590
HpyCH4V TGCA 2 cut(s) 192, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 177, 221
HpyF3I CTNAG 1 cut(s) 577
HpySE526I ACGT 3 cut(s) 87, 267, 590
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 26, 416, 500
Kpn2I TCCGGA 1 cut(s) 33
Ksp22I TGATCA 2 cut(s) 409, 544
Kzo9I GATC 5 cut(s) 45, 361, 409, 457, 544
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 9 cut(s) 32, 47, 56, 66, 87, 130, 181, 225, 331
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 372
MaeII ACGT 3 cut(s) 87, 267, 590
MaeIII GTNAC 3 cut(s) 200, 289, 401
MalI GATC 5 cut(s) 47, 363, 411, 459, 546
MboI GATC 5 cut(s) 45, 361, 409, 457, 544
MboII GAAGA 4 cut(s) 16, 252, 521, 572
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 1 cut(s) 332
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 3 cut(s) 288, 339, 415
MnlI CCTC 2 cut(s) 4, 550
MroI TCCGGA 1 cut(s) 33
MseI TTAA 1 cut(s) 525
MslI CAYNNNNRTG 2 cut(s) 498, 589
MspI CCGG 8 cut(s) 34, 43, 53, 74, 117, 168, 212, 318
MspR9I CCNGG 2 cut(s) 212, 318
MunI CAATTG 1 cut(s) 332
MwoI GCNNNNNNNGC 3 cut(s) 142, 177, 221
NciI CCSGG 2 cut(s) 212, 318
NdeII GATC 5 cut(s) 45, 361, 409, 457, 544
NlaIII CATG 3 cut(s) 26, 416, 500
NlaIV GGNNCC 3 cut(s) 47, 57, 135
NmuCI GTSAC 3 cut(s) 200, 289, 401
OliI CACNNNNGTG 1 cut(s) 589
PagI TCATGA 1 cut(s) 412
PkrI GCNGC 1 cut(s) 179
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PspN4I GGNNCC 3 cut(s) 47, 57, 135
PspPI GGNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 45
RseI CAYNNNNRTG 2 cut(s) 498, 589
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 5 cut(s) 45, 361, 409, 457, 544
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 212, 318
SduI GDGCHC 1 cut(s) 194
SetI ASST 5 cut(s) 90, 135, 255, 270, 593
SfaNI GCATC 1 cut(s) 502
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 2 cut(s) 498, 589
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 332
SsiI CCGC 4 cut(s) 107, 177, 298, 345
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 210, 316
StyI CCWWGG 4 cut(s) 129, 219, 225, 237
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 3 cut(s) 90, 270, 593
TaqI TCGA 2 cut(s) 99, 555
TasI AATT 1 cut(s) 332
TauI GCSGC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TseFI GTSAC 3 cut(s) 200, 289, 401
Tsp45I GTSAC 3 cut(s) 200, 289, 401
TspDTI ATGAA 2 cut(s) 17, 429
VneI GTGCAC 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 31
XcmI CCANNNNNNNNNTGG 2 cut(s) 283, 355
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.