RLG00000022234

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014170
Physical Location & Seq
Forward (+)
81852684 .. 81853259
576 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022234

Sequence Viewer

Length: 576 bp
ATGAAGAGAGAGGGTCGCCAACACGGAATGGTCCGGACATATCGGGTTCTACCTACCGAATTGAACCCGAGGCCCGAGACCCGACACGTCAACAAGTTCGACTCACTCCCCACGGCTGGGTTGTTCACTAAAGTGCCCAACAAGCCCAACAACCACTCCAAATACACGAGCAAGTGCACCAAGCCTAGGTGCACCGAGTGTCACATCCACCCGGCTAGTAAGTCCAAGGACAAGACCAAAGGGAACCAAAAGCTCAAGTCAAGCGATGTTTTGGCGAATCACAGGTTGGTCACATGGCGTGTTGTGGACGGACGATCCGGATTTAATTTTTCTGGATCGTCCGCCACGGGAATTTTGGATAATTTGTCTAGTGATTACCATGATAATAGTTGTGATAGTGACGATGAGGATGATGAAATCGATGATAATGAATCGAAGGTGGATTTCGATGATGTTGATGAAAAGGGAAGTAACGGTGAAGAGTCAGAAGAGGTGATGGGTTTCTGTAATGTGGATCATATGTTTGATTATCAAATTGAGGAAGATGGAGGTTGGTGCCTAGTGCCTTTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.56

Weight (kDa)

5.24

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40690
fragaria_vesca FvH4_6g52330
malus_domestica MD09G1018600.v1.1 MD17G1019100.v1.1
prunus_persica Prupe.3G298500_v2.0.a1 Prupe.3G298600_v2.0.a1
pyrus_communis pycom111g01550 pycom17g01590
rosa_chinensis RchiOBHm_Chr2g0173921
rosa_laevigata RLG00000022234
rosa_multiflora Rmu_co8413491.1_g000001
rosa_rugosa Rorug02G0573800
rosa_samantha Rh2AG652700 Rh2BG663900 Rh2CG628500 Rh2DG678800
rosa_wichuraiana Rw2G053620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 555
AccIII TCCGGA 2 cut(s) 33, 317
AciI CCGC 1 cut(s) 342
AclWI GGATC 3 cut(s) 309, 343, 522
AcsI RAATTY 1 cut(s) 351
AdeI CACNNNGTG 1 cut(s) 198
AfiI CCNNNNNNNGG 3 cut(s) 116, 117, 186
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 1 cut(s) 64
AjiI CACGTC 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AleI CACNNNNGTG 1 cut(s) 131
AluBI AGCT 1 cut(s) 253
AluI AGCT 1 cut(s) 253
Alw21I GWGCWC 2 cut(s) 179, 194
Alw26I GTCTC 1 cut(s) 71
Alw44I GTGCAC 2 cut(s) 175, 190
AlwI GGATC 3 cut(s) 309, 343, 522
Ama87I CYCGRG 2 cut(s) 67, 74
Aor13HI TCCGGA 2 cut(s) 33, 317
AoxI GGCC 1 cut(s) 71
ApaLI GTGCAC 2 cut(s) 175, 190
ApoI RAATTY 1 cut(s) 351
AspA2I CCTAGG 1 cut(s) 185
AspS9I GGNCC 2 cut(s) 31, 72
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 488, 505
AvaI CYCGRG 2 cut(s) 67, 74
AvaII GGWCC 1 cut(s) 31
AvrII CCTAGG 1 cut(s) 185
BaeGI GKGCMC 3 cut(s) 138, 179, 194
BanI GGYRCC 1 cut(s) 555
BauI CACGAG 1 cut(s) 166
Bbv12I GWGCWC 2 cut(s) 179, 194
BccI CCATC 2 cut(s) 490, 539
BceAI ACGGC 1 cut(s) 129
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 4 cut(s) 186, 216, 369, 560
BlnI CCTAGG 1 cut(s) 185
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 2 cut(s) 67, 74
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 31, 72
BmiI GGNNCC 2 cut(s) 245, 557
BmrFI CCNGG 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 212
Bsa29I ATCGAT 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 5 cut(s) 68, 111, 185, 225, 345
BsaWI WCCGGW 2 cut(s) 33, 317
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 117, 186
BseAI TCCGGA 2 cut(s) 33, 317
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 5 cut(s) 68, 111, 185, 225, 345
BseGI GGATG 2 cut(s) 204, 415
BseLI CCNNNNNNNGG 3 cut(s) 116, 117, 186
BseSI GKGCMC 3 cut(s) 138, 179, 194
BseYI CCCAGC 1 cut(s) 116
BshFI GGCC 1 cut(s) 73
BshNI GGYRCC 1 cut(s) 555
BshVI ATCGAT 1 cut(s) 420
BsiHKAI GWGCWC 2 cut(s) 179, 194
BsiHKCI CYCGRG 2 cut(s) 67, 74
BsiSI CCGG 3 cut(s) 34, 212, 318
BslI CCNNNNNNNGG 3 cut(s) 116, 117, 186
BsmAI GTCTC 1 cut(s) 71
BsnI GGCC 1 cut(s) 73
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 2 cut(s) 67, 74
Bsp1286I GDGCHC 3 cut(s) 138, 179, 194
Bsp13I TCCGGA 2 cut(s) 33, 317
Bsp143I GATC 3 cut(s) 314, 335, 514
BspACI CCGC 1 cut(s) 342
BspANI GGCC 1 cut(s) 73
BspDI ATCGAT 1 cut(s) 420
BspEI TCCGGA 2 cut(s) 33, 317
BspLI GGNNCC 2 cut(s) 245, 557
BspPI GGATC 3 cut(s) 309, 343, 522
BspT107I GGYRCC 1 cut(s) 555
BspTNI GGTCTC 1 cut(s) 71
BssECI CCNNGG 5 cut(s) 68, 111, 185, 225, 345
BssMI GATC 3 cut(s) 314, 335, 514
BssSI CACGAG 1 cut(s) 166
BssT1I CCWWGG 2 cut(s) 185, 225
Bst2BI CACGAG 1 cut(s) 166
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 2 cut(s) 474, 483
BstDSI CCRYGG 2 cut(s) 111, 345
BstF5I GGATG 2 cut(s) 204, 415
BstKTI GATC 3 cut(s) 317, 338, 517
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 3 cut(s) 314, 335, 514
BstMWI GCNNNNNNNGC 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 210
BstSLI GKGCMC 3 cut(s) 138, 179, 194
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 1 cut(s) 73
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 2 cut(s) 111, 345
BtgZI GCGATG 1 cut(s) 279
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 2 cut(s) 204, 415
Cfr13I GGNCC 2 cut(s) 31, 72
ClaI ATCGAT 1 cut(s) 420
CviAII CATG 2 cut(s) 294, 380
CviJI RGCY 6 cut(s) 73, 116, 145, 184, 215, 253
CviKI_1 RGCY 6 cut(s) 73, 116, 145, 184, 215, 253
DpnI GATC 3 cut(s) 316, 337, 516
DpnII GATC 3 cut(s) 314, 335, 514
DraIII CACNNNGTG 1 cut(s) 198
Eam1104I CTCTTC 2 cut(s) 474, 483
EarI CTCTTC 2 cut(s) 474, 483
EciI GGCGGA 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 185, 225
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 31
Eco88I CYCGRG 2 cut(s) 67, 74
EcoT14I CCWWGG 2 cut(s) 185, 225
ErhI CCWWGG 2 cut(s) 185, 225
FaeI CATG 2 cut(s) 297, 383
FaiI YATR 5 cut(s) 40, 295, 381, 519, 521
FatI CATG 2 cut(s) 293, 379
FauNDI CATATG 1 cut(s) 519
FokI GGATG 2 cut(s) 191, 422
FspBI CTAG 4 cut(s) 186, 216, 369, 560
GsaI CCCAGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 73
HapII CCGG 3 cut(s) 34, 212, 318
Hin1II CATG 2 cut(s) 297, 383
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 4 cut(s) 101, 277, 431, 482
HpaII CCGG 3 cut(s) 34, 212, 318
HphI GGTGA 2 cut(s) 488, 505
Hpy166II GTNNAC 5 cut(s) 91, 126, 177, 192, 307
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 3 cut(s) 34, 318, 333
Hpy8I GTNNAC 5 cut(s) 91, 126, 177, 192, 307
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 177, 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 142
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 2 cut(s) 297, 383
Kpn2I TCCGGA 2 cut(s) 33, 317
Kzo9I GATC 3 cut(s) 314, 335, 514
LpnPI CCDG 6 cut(s) 47, 102, 225, 268, 318, 331
MaeI CTAG 4 cut(s) 186, 216, 369, 560
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 4 cut(s) 200, 289, 398, 470
MalI GATC 3 cut(s) 316, 337, 516
MboI GATC 3 cut(s) 314, 335, 514
MboII GAAGA 4 cut(s) 16, 491, 500, 554
MhlI GDGCHC 3 cut(s) 138, 179, 194
MluCI AATT 5 cut(s) 59, 325, 351, 361, 534
MlyI GAGTC 2 cut(s) 95, 491
MnlI CCTC 6 cut(s) 4, 63, 400, 484, 532, 542
MroI TCCGGA 2 cut(s) 33, 317
MseI TTAA 2 cut(s) 324, 569
MslI CAYNNNNRTG 1 cut(s) 131
MspI CCGG 3 cut(s) 34, 212, 318
MspR9I CCNGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 142
NciI CCSGG 1 cut(s) 212
NdeI CATATG 1 cut(s) 519
NdeII GATC 3 cut(s) 314, 335, 514
NlaIII CATG 2 cut(s) 297, 383
NlaIV GGNNCC 2 cut(s) 245, 557
NmuCI GTSAC 3 cut(s) 200, 289, 398
OliI CACNNNNGTG 1 cut(s) 131
PfeI GAWTC 2 cut(s) 277, 431
PleI GAGTC 2 cut(s) 95, 490
PpsI GAGTC 2 cut(s) 95, 490
PspFI CCCAGC 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 245, 557
PspPI GGNCC 2 cut(s) 31, 72
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 2 cut(s) 324, 569
Sau3AI GATC 3 cut(s) 314, 335, 514
Sau96I GGNCC 2 cut(s) 31, 72
SchI GAGTC 2 cut(s) 95, 491
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 3 cut(s) 138, 179, 194
SetI ASST 8 cut(s) 55, 90, 191, 255, 287, 441, 495, 553
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 5 cut(s) 59, 325, 351, 361, 534
SsiI CCGC 1 cut(s) 342
SspMI CTAG 4 cut(s) 186, 216, 369, 560
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 2 cut(s) 185, 225
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 1 cut(s) 90
TaqI TCGA 4 cut(s) 99, 420, 434, 447
TasI AATT 5 cut(s) 59, 325, 351, 361, 534
TfiI GAWTC 2 cut(s) 277, 431
Tru1I TTAA 2 cut(s) 324, 569
Tru9I TTAA 2 cut(s) 324, 569
TseFI GTSAC 3 cut(s) 200, 289, 398
Tsp45I GTSAC 3 cut(s) 200, 289, 398
TspDTI ATGAA 4 cut(s) 17, 429, 444, 474
TspGWI ACGGA 2 cut(s) 39, 324
VneI GTGCAC 2 cut(s) 175, 190
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 1 cut(s) 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XmaJI CCTAGG 1 cut(s) 185
XspI CTAG 4 cut(s) 186, 216, 369, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.