pycom111g01550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0014170
Physical Location & Seq
Reverse (-)
971282 .. 971962
681 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01550.1

Sequence Viewer

Length: 681 bp
ATGCAGTATATAAAAGAGAAGCAAAATATTAACAACTCCATCATTTTTTCGTTCTTTGCTTTTAGCCTACAACCCAATTTGTCAACAATGAAGAGAGAGGGTCGCCAGCATGGAATGGTCCGGACACACCGGATCCTACCGGAGCCGTTGAACCCGAGACCGGCAACCCGACACGTCAACAAGTTCGAGTCTCCGCCCACGGCCGGATTATTCACCAAGGTGTCAACCAAGCCCACCAACCACTCTAAGTTCACCGGCAAGTGCGGCAAGCCCAAGTGCACGGAGTGTCACATTCACCCGGCTTCCAAGGCCAAGGACAAGACCAAGGGCAACCGAAAGCTCAAGTCATCCGACGTCGTTTCCAATCATCGGTTGGTCACTTGGCGGGTCGTGGACGGGCAACCCGGCTTGAATAGTCAATTGGGTTTGTCCGCCACGGGGATGTTGGATCGTCTCTCTAGGGATTATGAGAATGAGTATGATGATGGTGACGATGATCATGAAATCAATGGTTCTGCAATTGGATATTATTATAGAGATGATGATCGGACGGTGGAAAATAATGTTGATGACATTGAGCATCGTGGTGATGATGAAGAGATGGGTTTATTTAATGTGGATTGTGTGATTGATCATCAAATCGAGGAAGATGATGGTTGGTGTTTAGTGCCACAAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.7

Weight (kDa)

6.06

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40690
fragaria_vesca FvH4_6g52330
malus_domestica MD09G1018600.v1.1 MD17G1019100.v1.1
prunus_persica Prupe.3G298500_v2.0.a1 Prupe.3G298600_v2.0.a1
pyrus_communis pycom111g01550 pycom17g01590
rosa_chinensis RchiOBHm_Chr2g0173921
rosa_laevigata RLG00000022234
rosa_multiflora Rmu_co8413491.1_g000001
rosa_rugosa Rorug02G0573800
rosa_samantha Rh2AG652700 Rh2BG663900 Rh2CG628500 Rh2DG678800
rosa_wichuraiana Rw2G053620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
AccIII TCCGGA 1 cut(s) 120
AciI CCGC 4 cut(s) 194, 264, 385, 432
AclWI GGATC 3 cut(s) 127, 140, 456
AcoI YGGCCR 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 354
AdeI CACNNNGTG 1 cut(s) 285
AfiI CCNNNNNNNGG 4 cut(s) 160, 203, 369, 438
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 2 cut(s) 151, 412
AjiI CACGTC 1 cut(s) 175
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AleI CACNNNNGTG 2 cut(s) 218, 676
AluBI AGCT 1 cut(s) 340
AluI AGCT 1 cut(s) 340
Alw21I GWGCWC 1 cut(s) 281
Alw26I GTCTC 3 cut(s) 151, 195, 458
Alw44I GTGCAC 1 cut(s) 277
AlwI GGATC 3 cut(s) 127, 140, 456
Ama87I CYCGRG 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 120
AoxI GGCC 2 cut(s) 201, 309
ApaLI GTGCAC 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 118
AsuC2I CCSGG 2 cut(s) 299, 405
AsuHPI GGTGA 5 cut(s) 205, 244, 287, 500, 599
AvaI CYCGRG 1 cut(s) 154
AvaII GGWCC 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 281
BamHI GGATCC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 281
BccI CCATC 4 cut(s) 47, 479, 595, 647
BceAI ACGGC 2 cut(s) 130, 216
BclI TGATCA 2 cut(s) 496, 631
BcnI CCSGG 2 cut(s) 299, 405
BcoDI GTCTC 3 cut(s) 151, 195, 458
BfaI CTAG 1 cut(s) 459
BisI GCNGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 266
Bme1390I CCNGG 2 cut(s) 299, 405
Bme18I GGWCC 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 154
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 2 cut(s) 134, 144
BmrFI CCNGG 2 cut(s) 299, 405
BmsI GCATC 1 cut(s) 589
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 2 cut(s) 299, 405
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 6 cut(s) 198, 216, 306, 312, 324, 435
BsaWI WCCGGW 3 cut(s) 120, 129, 139
Bsc4I CCNNNNNNNGG 4 cut(s) 160, 203, 369, 438
Bse118I RCCGGY 2 cut(s) 160, 254
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 120
BseDI CCNNGG 6 cut(s) 198, 216, 306, 312, 324, 435
BseGI GGATG 2 cut(s) 347, 447
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 4 cut(s) 160, 203, 369, 438
BseSI GKGCMC 1 cut(s) 281
BseX3I CGGCCG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 203, 311
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 281
BsiHKCI CYCGRG 1 cut(s) 154
BsiSI CCGG 8 cut(s) 121, 130, 140, 161, 204, 255, 299, 405
BslI CCNNNNNNNGG 4 cut(s) 160, 203, 369, 438
BsmAI GTCTC 3 cut(s) 151, 195, 458
BsmBI CGTCTC 1 cut(s) 458
BsnI GGCC 2 cut(s) 203, 311
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 281
Bsp13I TCCGGA 1 cut(s) 120
Bsp143I GATC 5 cut(s) 132, 448, 496, 544, 631
BspACI CCGC 4 cut(s) 194, 264, 385, 432
BspANI GGCC 2 cut(s) 203, 311
BspEI TCCGGA 1 cut(s) 120
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 2 cut(s) 134, 144
BspPI GGATC 3 cut(s) 127, 140, 456
BspTNI GGTCTC 1 cut(s) 151
BsrFI RCCGGY 2 cut(s) 160, 254
BssAI RCCGGY 2 cut(s) 160, 254
BssECI CCNNGG 6 cut(s) 198, 216, 306, 312, 324, 435
BssMI GATC 5 cut(s) 132, 448, 496, 544, 631
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 4 cut(s) 216, 306, 312, 324
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 2 cut(s) 86, 591
BstACI GRCGYC 1 cut(s) 354
BstC8I GCNNGC 2 cut(s) 107, 269
BstDEI CTNAG 1 cut(s) 246
BstDSI CCRYGG 2 cut(s) 198, 435
BstF5I GGATG 2 cut(s) 347, 447
BstKTI GATC 5 cut(s) 135, 451, 499, 547, 634
BstMAI GTCTC 3 cut(s) 151, 195, 458
BstMBI GATC 5 cut(s) 132, 448, 496, 544, 631
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 264, 308
BstSCI CCNGG 2 cut(s) 297, 403
BstSLI GKGCMC 1 cut(s) 281
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
BstZI CGGCCG 1 cut(s) 201
BsuRI GGCC 2 cut(s) 203, 311
BtgI CCRYGG 2 cut(s) 198, 435
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 2 cut(s) 347, 447
Cac8I GCNNGC 2 cut(s) 107, 269
CciI TCATGA 1 cut(s) 499
Cfr10I RCCGGY 2 cut(s) 160, 254
Cfr13I GGNCC 1 cut(s) 118
CviAII CATG 2 cut(s) 110, 500
CviJI RGCY 9 cut(s) 66, 145, 203, 232, 271, 302, 311, 340, 408
CviKI_1 RGCY 9 cut(s) 66, 145, 203, 232, 271, 302, 311, 340, 408
DdeI CTNAG 1 cut(s) 246
DpnI GATC 5 cut(s) 134, 450, 498, 546, 633
DpnII GATC 5 cut(s) 132, 448, 496, 544, 631
DraIII CACNNNGTG 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 201
EagI CGGCCG 1 cut(s) 201
Eam1104I CTCTTC 2 cut(s) 86, 591
EarI CTCTTC 2 cut(s) 86, 591
EciI GGCGGA 2 cut(s) 183, 421
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 4 cut(s) 216, 306, 312, 324
Eco31I GGTCTC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 118
Eco52I CGGCCG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 154
EcoT14I CCWWGG 4 cut(s) 216, 306, 312, 324
ErhI CCWWGG 4 cut(s) 216, 306, 312, 324
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 2 cut(s) 113, 503
FaiI YATR 7 cut(s) 9, 11, 111, 468, 480, 501, 534
FatI CATG 2 cut(s) 109, 499
FauI CCCGC 1 cut(s) 378
FbaI TGATCA 2 cut(s) 496, 631
Fnu4HI GCNGC 1 cut(s) 265
FokI GGATG 2 cut(s) 334, 454
Fsp4HI GCNGC 1 cut(s) 265
FspBI CTAG 1 cut(s) 459
GluI GCNGC 1 cut(s) 265
HaeIII GGCC 2 cut(s) 203, 311
HapII CCGG 8 cut(s) 121, 130, 140, 161, 204, 255, 299, 405
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 2 cut(s) 113, 503
HincII GTYRAC 3 cut(s) 84, 178, 225
HindII GTYRAC 3 cut(s) 84, 178, 225
HinfI GANTC 1 cut(s) 188
HpaII CCGG 8 cut(s) 121, 130, 140, 161, 204, 255, 299, 405
HphI GGTGA 5 cut(s) 205, 244, 287, 500, 599
Hpy166II GTNNAC 6 cut(s) 84, 178, 225, 252, 279, 394
Hpy188I TCNGA 2 cut(s) 352, 549
Hpy188III TCNNGA 2 cut(s) 121, 500
Hpy8I GTNNAC 6 cut(s) 84, 178, 225, 252, 279, 394
Hpy99I CGWCG 2 cut(s) 356, 359
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4IV ACGT 3 cut(s) 174, 354, 677
HpyCH4V TGCA 3 cut(s) 4, 279, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 308
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 3 cut(s) 174, 354, 677
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 2 cut(s) 113, 503
Kpn2I TCCGGA 1 cut(s) 120
Ksp22I TGATCA 2 cut(s) 496, 631
Kzo9I GATC 5 cut(s) 132, 448, 496, 544, 631
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 9 cut(s) 119, 134, 143, 153, 174, 217, 268, 312, 418
LweI GCATC 1 cut(s) 589
MaeI CTAG 1 cut(s) 459
MaeII ACGT 3 cut(s) 174, 354, 677
MaeIII GTNAC 3 cut(s) 287, 376, 488
MalI GATC 5 cut(s) 134, 450, 498, 546, 633
MboI GATC 5 cut(s) 132, 448, 496, 544, 631
MboII GAAGA 3 cut(s) 103, 608, 659
MfeI CAATTG 2 cut(s) 419, 519
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 281
MluCI AATT 3 cut(s) 76, 419, 519
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 375, 426
MnlI CCTC 2 cut(s) 91, 637
MroI TCCGGA 1 cut(s) 120
MseI TTAA 2 cut(s) 30, 612
MslI CAYNNNNRTG 4 cut(s) 218, 440, 585, 676
MspI CCGG 8 cut(s) 121, 130, 140, 161, 204, 255, 299, 405
MspR9I CCNGG 2 cut(s) 299, 405
MunI CAATTG 2 cut(s) 419, 519
MwoI GCNNNNNNNGC 2 cut(s) 264, 308
NciI CCSGG 2 cut(s) 299, 405
NdeII GATC 5 cut(s) 132, 448, 496, 544, 631
NlaIII CATG 2 cut(s) 113, 503
NlaIV GGNNCC 2 cut(s) 134, 144
NmuCI GTSAC 3 cut(s) 287, 376, 488
OliI CACNNNNGTG 2 cut(s) 218, 676
PagI TCATGA 1 cut(s) 499
PkrI GCNGC 1 cut(s) 266
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PspN4I GGNNCC 2 cut(s) 134, 144
PspPI GGNCC 1 cut(s) 118
PsuI RGATCY 1 cut(s) 132
RseI CAYNNNNRTG 4 cut(s) 218, 440, 585, 676
SaqAI TTAA 2 cut(s) 30, 612
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 5 cut(s) 132, 448, 496, 544, 631
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 299, 405
SduI GDGCHC 1 cut(s) 281
SetI ASST 5 cut(s) 177, 222, 342, 357, 680
SfaNI GCATC 1 cut(s) 589
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 4 cut(s) 218, 440, 585, 676
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 3 cut(s) 76, 419, 519
SsiI CCGC 4 cut(s) 194, 264, 385, 432
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 297, 403
StyI CCWWGG 4 cut(s) 216, 306, 312, 324
TaaI ACNGT 1 cut(s) 553
TaiI ACGT 3 cut(s) 177, 357, 680
TaqI TCGA 2 cut(s) 186, 642
TasI AATT 3 cut(s) 76, 419, 519
TauI GCSGC 1 cut(s) 267
Tru1I TTAA 2 cut(s) 30, 612
Tru9I TTAA 2 cut(s) 30, 612
TseFI GTSAC 3 cut(s) 287, 376, 488
Tsp45I GTSAC 3 cut(s) 287, 376, 488
TspDTI ATGAA 3 cut(s) 104, 516, 609
TspGWI ACGGA 1 cut(s) 296
VneI GTGCAC 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 118
XcmI CCANNNNNNNNNTGG 2 cut(s) 370, 442
XspI CTAG 1 cut(s) 459
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.