MD09G1020100.v1.1

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014375
Physical Location & Seq
Forward (+)
1222327 .. 1225132
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1020100.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGGCATCTCCGCGCCGACGACTGTCTTCTCTCTTATATTCTGTGTCTCCTCTCCTTCTCTCTCGCTCCTCCTATTCCTATTGCTCTCTCTTCCACGCATTACCCTTTTGCCCTCGTCTCTCTCTCTCCTCTTCATCCATGGCTCCAGAACTTCAGGAGGAAAATTACACTACTGGCCCTGCTGAACCTCCCCAGAAAACCAGAGTCACCGTCGTAGGCAGTGGCAACTGGGGCAGTGTTGCCGCCAAGCTCATTGCCTCCAACACCCTCAGGCTCTCTTCTTTCCACGATGAAGTGAGAATGTGGGTGTTTGAGGAAACGTTACCAAGTGGTGAGAAGCTCACAGATGCCATCAACCGCAACAATGAGAATGTGAAATATCTGCCTGGCATTAAGCTGGGGAAAAATGTTGTTGCAGACCCGGACCTTGAGAATGCAGTGAACGGTGCGAACATGTTGGTGTTTGTTACTCCACATCAATTTATGGAGGGTATATGCAGGAGGCTTGTTGGGAAGGTAAAAGCAGATGTGGAGGCGATTTCCCTCATCAAAGGAATGGAGGTCAAGATGGAAGGCCCATGCATGATCTCGACACTCATCTCAGATCAATTGGGTATTAATTGTTGTGTTCTGATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGTGAAGCCACGGTTGGATACAGAGAGAAAAAAGAGATTGCACAGAAATGGGTTCAACTGTTTAGTACTTCCTACTTCATAGTCACACCTGTCCAAGACGTGGAAGGAGTAGAACTGTGTGGAACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGATGGATTGGAGATGGGAAATAACACGAAGGCTGCAATTATGAGAATTGGTCTTAGGGAGATGAAGGCATTTTCCAAGATGTTATTTTCATCTGTCAAGGACACCACCTTCTTCGAAAGCTGTGGCGTCGCTGATCTCATCACAACATGCCTGGGAGGAAGAAACAGGAAAGTGGCAGAAGCTTTTGCAAGGAGTGAAGGGAAAAGATCGTTTGATGAGCTCGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTCTCAACCGCAAAAGAGGTTTACGAAGTTTTAAGCCACCGTGGGTGGCTAGATTTCTTCCCACTTTTTGCAACAGTTCATGAGATCTGCATTGGCACTCTTCCTCCAACAGCCATAGTTGAACACAGTGAGCGCACGCCTAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

415

Amino Acids

45.49

Weight (kDa)

6.37

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 69 - 236 4.6e-41 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 258 - 406 2e-45 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 818
AccB7I CCANNNNNTGG 1 cut(s) 775
AccII CGCG 1 cut(s) 13
AciI CCGC 4 cut(s) 11, 243, 358, 1104
AclI AACGTT 1 cut(s) 320
AcoI YGGCCR 1 cut(s) 816
AcsI RAATTY 1 cut(s) 671
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 963
AfaI GTAC 1 cut(s) 742
AfiI CCNNNNNNNGG 1 cut(s) 775
AflIII ACRYGT 1 cut(s) 453
AgsI TTSAA 2 cut(s) 731, 1217
AjiI CACGTC 1 cut(s) 775
AjnI CCWGG 2 cut(s) 385, 987
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
AluBI AGCT 7 cut(s) 250, 340, 397, 650, 957, 1019, 1057
AluI AGCT 7 cut(s) 250, 340, 397, 650, 957, 1019, 1057
Alw21I GWGCWC 1 cut(s) 1059
Alw26I GTCTC 3 cut(s) 51, 122, 1102
AoxI GGCC 3 cut(s) 175, 574, 816
ApeKI GCWGC 3 cut(s) 824, 869, 1072
ApoI RAATTY 1 cut(s) 671
AseI ATTAAT 1 cut(s) 618
AspLEI GCGC 2 cut(s) 15, 1230
AspS9I GGNCC 3 cut(s) 176, 424, 575
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 199, 344
AsuII TTCGAA 1 cut(s) 951
AvaII GGWCC 1 cut(s) 424
AxyI CCTNAGG 1 cut(s) 269
BalI TGGCCA 1 cut(s) 818
BanII GRGCYC 1 cut(s) 1059
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 1059
BbvI GCAGC 3 cut(s) 836, 856, 1059
BccI CCATC 5 cut(s) 359, 562, 628, 833, 844
BciT130I CCWGG 2 cut(s) 387, 989
BciVI GTATCC 1 cut(s) 686
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 3 cut(s) 51, 122, 1102
BfaI CTAG 1 cut(s) 1145
BfmI CTRYAG 1 cut(s) 1073
BfuAI ACCTGC 1 cut(s) 818
BfuI GTATCC 1 cut(s) 686
BglII AGATCT 1 cut(s) 1179
BisI GCNGC 4 cut(s) 243, 825, 870, 1073
BlsI GCNGC 4 cut(s) 244, 826, 871, 1074
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 3 cut(s) 387, 422, 989
Bme18I GGWCC 1 cut(s) 424
BmgBI CACGTC 1 cut(s) 775
BmgT120I GGNCC 3 cut(s) 176, 424, 575
BmiI GGNNCC 2 cut(s) 144, 799
BmrFI CCNGG 3 cut(s) 387, 422, 989
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 14, 337
BmuI ACTGGG 1 cut(s) 238
BoxI GACNNNNGTC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 129
Bpu14I TTCGAA 1 cut(s) 951
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 963
BsaJI CCNNGG 4 cut(s) 138, 684, 988, 1135
BsaXI ACNNNNNCTCC 2 cut(s) 1183, 1213
Bsc4I CCNNNNNNNGG 1 cut(s) 775
Bse1I ACTGG 2 cut(s) 178, 233
Bse21I CCTNAGG 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 2 cut(s) 387, 989
BseDI CCNNGG 4 cut(s) 138, 684, 988, 1135
BseGI GGATG 2 cut(s) 134, 844
BseLI CCNNNNNNNGG 1 cut(s) 775
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 283, 615
BseNI ACTGG 2 cut(s) 178, 233
BseRI GAGGAG 3 cut(s) 39, 58, 118
BseXI GCAGC 3 cut(s) 836, 856, 1059
BseYI CCCAGC 1 cut(s) 397
Bsh1236I CGCG 1 cut(s) 13
BshFI GGCC 3 cut(s) 177, 576, 818
BsiHKAI GWGCWC 1 cut(s) 1059
BsiSI CCGG 1 cut(s) 422
BslI CCNNNNNNNGG 1 cut(s) 775
BsmAI GTCTC 3 cut(s) 51, 122, 1102
BsmBI CGTCTC 1 cut(s) 122
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 3 cut(s) 177, 576, 818
Bsp119I TTCGAA 1 cut(s) 951
Bsp1286I GDGCHC 1 cut(s) 1059
Bsp143I GATC 5 cut(s) 585, 604, 970, 1043, 1179
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 4 cut(s) 11, 243, 358, 1104
BspANI GGCC 3 cut(s) 177, 576, 818
BspCNI CTCAG 2 cut(s) 282, 614
BspFNI CGCG 1 cut(s) 13
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 2 cut(s) 144, 799
BspMAI CTGCAG 1 cut(s) 1077
BspMI ACCTGC 1 cut(s) 818
BspT104I TTCGAA 1 cut(s) 951
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 2 cut(s) 178, 233
BssECI CCNNGG 4 cut(s) 138, 684, 988, 1135
BssMI GATC 5 cut(s) 585, 604, 970, 1043, 1179
BssNI GRCGYC 1 cut(s) 963
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 2 cut(s) 387, 989
Bst4CI ACNGT 9 cut(s) 24, 211, 446, 688, 735, 792, 1136, 1171, 1223
Bst6I CTCTTC 4 cut(s) 95, 136, 283, 1200
BstACI GRCGYC 1 cut(s) 963
BstBI TTCGAA 1 cut(s) 951
BstC8I GCNNGC 1 cut(s) 1232
BstDEI CTNAG 3 cut(s) 269, 601, 890
BstDSI CCRYGG 3 cut(s) 138, 684, 1135
BstF5I GGATG 2 cut(s) 134, 844
BstFNI CGCG 1 cut(s) 13
BstHHI GCGC 2 cut(s) 15, 1230
BstKTI GATC 5 cut(s) 588, 607, 973, 1046, 1182
BstMAI GTCTC 3 cut(s) 51, 122, 1102
BstMBI GATC 5 cut(s) 585, 604, 970, 1043, 1179
BstMWI GCNNNNNNNGC 3 cut(s) 231, 647, 824
BstNI CCWGG 2 cut(s) 387, 989
BstNSI RCATGY 2 cut(s) 457, 987
BstPAI GACNNNNGTC 1 cut(s) 22
BstSCI CCNGG 3 cut(s) 385, 420, 987
BstSFI CTRYAG 1 cut(s) 1073
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 3 cut(s) 836, 856, 1059
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 1179
BstYI RGATCY 1 cut(s) 1179
Bsu36I CCTNAGG 1 cut(s) 269
BsuI GTATCC 1 cut(s) 686
BsuRI GGCC 3 cut(s) 177, 576, 818
BtgI CCRYGG 3 cut(s) 138, 684, 1135
BtrI CACGTC 1 cut(s) 775
BtsCI GGATG 2 cut(s) 134, 844
BtsI GCAGTG 3 cut(s) 226, 241, 444
BtsIMutI CAGTG 4 cut(s) 226, 241, 444, 1228
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 1 cut(s) 1232
CciI TCATGA 1 cut(s) 1174
CfoI GCGC 2 cut(s) 15, 1230
Cfr13I GGNCC 3 cut(s) 176, 424, 575
CseI GACGC 1 cut(s) 952
Csp6I GTAC 1 cut(s) 741
CviAII CATG 6 cut(s) 139, 454, 579, 583, 984, 1175
CviQI GTAC 1 cut(s) 741
DdeI CTNAG 3 cut(s) 269, 601, 890
DpnI GATC 5 cut(s) 587, 606, 972, 1045, 1181
DpnII GATC 5 cut(s) 585, 604, 970, 1043, 1179
EaeI YGGCCR 1 cut(s) 816
Eam1104I CTCTTC 4 cut(s) 95, 136, 283, 1200
EarI CTCTTC 4 cut(s) 95, 136, 283, 1200
Ecl136II GAGCTC 1 cut(s) 1057
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 1059
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 1057
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 1057
EcoRII CCWGG 2 cut(s) 385, 987
EcoT14I CCWWGG 1 cut(s) 138
EcoT22I ATGCAT 1 cut(s) 584
EcoT38I GRGCYC 1 cut(s) 1059
ErhI CCWWGG 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 6 cut(s) 142, 457, 582, 586, 987, 1178
FatI CATG 6 cut(s) 138, 453, 578, 582, 983, 1174
Fnu4HI GCNGC 4 cut(s) 243, 825, 870, 1073
FokI GGATG 2 cut(s) 121, 851
FriOI GRGCYC 1 cut(s) 1059
Fsp4HI GCNGC 4 cut(s) 243, 825, 870, 1073
FspBI CTAG 1 cut(s) 1145
GlaI GCGC 2 cut(s) 14, 1229
GluI GCNGC 4 cut(s) 243, 825, 870, 1073
GsaI CCCAGC 1 cut(s) 401
GsuI CTGGAG 1 cut(s) 129
HaeIII GGCC 3 cut(s) 177, 576, 818
HapII CCGG 1 cut(s) 422
HgaI GACGC 1 cut(s) 952
HhaI GCGC 2 cut(s) 15, 1230
Hin1I GRCGYC 1 cut(s) 963
Hin1II CATG 6 cut(s) 142, 457, 582, 586, 987, 1178
Hin6I GCGC 2 cut(s) 13, 1228
HinP1I GCGC 2 cut(s) 13, 1228
HindIII AAGCTT 1 cut(s) 1017
HinfI GANTC 1 cut(s) 204
HpaII CCGG 1 cut(s) 422
HphI GGTGA 2 cut(s) 199, 344
Hpy166II GTNNAC 2 cut(s) 442, 1117
Hpy188I TCNGA 2 cut(s) 604, 633
Hpy188III TCNNGA 5 cut(s) 146, 155, 565, 589, 1175
Hpy8I GTNNAC 2 cut(s) 442, 1117
Hpy99I CGWCG 3 cut(s) 21, 215, 968
HpyAV CCTTC 8 cut(s) 65, 508, 566, 773, 859, 895, 955, 1028
HpyCH4III ACNGT 9 cut(s) 24, 211, 446, 688, 735, 792, 1136, 1171, 1223
HpyCH4IV ACGT 2 cut(s) 320, 774
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 647, 824
HpyF3I CTNAG 3 cut(s) 269, 601, 890
HpySE526I ACGT 2 cut(s) 320, 774
Hsp92I GRCGYC 1 cut(s) 963
Hsp92II CATG 6 cut(s) 142, 457, 582, 586, 987, 1178
HspAI GCGC 2 cut(s) 13, 1228
Kzo9I GATC 5 cut(s) 585, 604, 970, 1043, 1179
LmnI GCTCC 3 cut(s) 71, 148, 638
Lsp1109I GCAGC 3 cut(s) 836, 856, 1059
LweI GCATC 2 cut(s) 14, 337
MaeI CTAG 1 cut(s) 1145
MaeII ACGT 2 cut(s) 320, 774
MaeIII GTNAC 4 cut(s) 205, 321, 466, 757
MalI GATC 5 cut(s) 587, 606, 972, 1045, 1181
MboI GATC 5 cut(s) 585, 604, 970, 1043, 1179
MboII GAAGA 8 cut(s) 18, 82, 123, 270, 940, 1008, 1144, 1187
MfeI CAATTG 1 cut(s) 608
MflI RGATCY 1 cut(s) 1179
MhlI GDGCHC 1 cut(s) 1059
MlsI TGGCCA 1 cut(s) 818
MluCI AATT 8 cut(s) 163, 479, 608, 619, 671, 873, 882, 1085
MluNI TGGCCA 1 cut(s) 818
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 3 cut(s) 285, 670, 1226
Mox20I TGGCCA 1 cut(s) 818
Mph1103I ATGCAT 1 cut(s) 584
MscI TGGCCA 1 cut(s) 818
MseI TTAA 3 cut(s) 393, 618, 1127
MslI CAYNNNNRTG 2 cut(s) 458, 721
Msp20I TGGCCA 1 cut(s) 818
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 3 cut(s) 387, 422, 989
MunI CAATTG 1 cut(s) 608
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 2 cut(s) 387, 989
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 3 cut(s) 231, 647, 824
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 1 cut(s) 138
NdeII GATC 5 cut(s) 585, 604, 970, 1043, 1179
NlaIII CATG 6 cut(s) 142, 457, 582, 586, 987, 1178
NlaIV GGNNCC 2 cut(s) 144, 799
NmuCI GTSAC 2 cut(s) 205, 757
NsiI ATGCAT 1 cut(s) 584
NspI RCATGY 2 cut(s) 457, 987
NspV TTCGAA 1 cut(s) 951
PagI TCATGA 1 cut(s) 1174
PciI ACATGT 1 cut(s) 453
PctI GAATGC 1 cut(s) 439
PflMI CCANNNNNTGG 1 cut(s) 775
PkrI GCNGC 4 cut(s) 244, 826, 871, 1074
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
PscI ACATGT 1 cut(s) 453
PshAI GACNNNNGTC 1 cut(s) 22
PshBI ATTAAT 1 cut(s) 618
Psp124BI GAGCTC 1 cut(s) 1059
Psp1406I AACGTT 1 cut(s) 320
Psp6I CCWGG 2 cut(s) 385, 987
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 2 cut(s) 385, 987
PspN4I GGNNCC 2 cut(s) 144, 799
PspPI GGNCC 3 cut(s) 176, 424, 575
PstI CTGCAG 1 cut(s) 1077
PsuI RGATCY 1 cut(s) 1179
RsaI GTAC 1 cut(s) 742
RsaNI GTAC 1 cut(s) 741
RseI CAYNNNNRTG 2 cut(s) 458, 721
SacI GAGCTC 1 cut(s) 1059
SaqAI TTAA 3 cut(s) 393, 618, 1127
SatI GCNGC 4 cut(s) 243, 825, 870, 1073
Sau3AI GATC 5 cut(s) 585, 604, 970, 1043, 1179
Sau96I GGNCC 3 cut(s) 176, 424, 575
ScaI AGTACT 1 cut(s) 742
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 3 cut(s) 387, 422, 989
SduI GDGCHC 1 cut(s) 1059
SfaNI GCATC 2 cut(s) 14, 337
SfcI CTRYAG 1 cut(s) 1073
SfuI TTCGAA 1 cut(s) 951
SinI GGWCC 1 cut(s) 424
SmiMI CAYNNNNRTG 2 cut(s) 458, 721
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 8 cut(s) 163, 479, 608, 619, 671, 873, 882, 1085
SsiI CCGC 4 cut(s) 11, 243, 358, 1104
SspMI CTAG 1 cut(s) 1145
SstI GAGCTC 1 cut(s) 1059
StyD4I CCNGG 3 cut(s) 385, 420, 987
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 9 cut(s) 24, 211, 446, 688, 735, 792, 1136, 1171, 1223
TaiI ACGT 2 cut(s) 323, 777
TaqI TCGA 3 cut(s) 590, 951, 1059
TasI AATT 8 cut(s) 163, 479, 608, 619, 671, 873, 882, 1085
TatI WGTACW 1 cut(s) 740
TauI GCSGC 1 cut(s) 245
Tru1I TTAA 3 cut(s) 393, 618, 1127
Tru9I TTAA 3 cut(s) 393, 618, 1127
TscAI CASTG 4 cut(s) 226, 241, 444, 1228
TseFI GTSAC 2 cut(s) 205, 757
TseI GCWGC 3 cut(s) 824, 869, 1072
Tsp45I GTSAC 2 cut(s) 205, 757
TspDTI ATGAA 6 cut(s) 123, 306, 742, 914, 915, 1163
TspRI CASTG 4 cut(s) 226, 241, 444, 1228
Van91I CCANNNNNTGG 1 cut(s) 775
VpaK11BI GGWCC 1 cut(s) 424
VspI ATTAAT 1 cut(s) 618
XapI RAATTY 1 cut(s) 671
XceI RCATGY 2 cut(s) 457, 987
XspI CTAG 1 cut(s) 1145
ZrmI AGTACT 1 cut(s) 742
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.