MD17G1020700.v1.1

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014375
Physical Location & Seq
Forward (+)
1560237 .. 1562888
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1020700.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGAAAAACATAAAAGGTGTAGTATATGGTAGTAATTTTAATTTATTAGATCATAGAGGAGATCGCCATATTTGTCTTGGAGTTCAATACAATTTAGGCATCGTTTGTGACGAAACGTGTATGGCATATCCGCCCCGACGGGTGTCTTCCCTCTTATATTCTCTGTCTCCTCTCCTTCTCTCTCGCTCCTCCTATACCTTTTCCTCTATCATCTACGCGTTGCCCTTTTGCCCTCCTCTCTTTCTCTCTCCTTTCCTCTCTCTCTCCTCTTCATCCATGGCTCCAGAACTCCAACAGGAGGAAGATTACACTACTGGCCCTGAAGCTCCTCACAAAACCAGAGTCACCGTCGTCGGCAGTGGCAACTGGGGTAGTGTTGCCGCCAAACTCATTGCCTCCAACACCCTCGGGCTCTCTTCCTTCCACGATGAAGTGAGAATGTGGGTGTTTGAGGAAACATTACCAAGTGGTGAGAAGCTCACAGATGCCATCAACCGCAACAATGAAAATGTGAAATACCTGCCCGGCATTAAGCTGGGGAAAAATGTTGTTGCAGACCCAGACCTTGAGAATGCAGTGAACGGTGCGAACATGTTGGTATTTGTTACTCCACATCAATTTATGGAGGGTATATGCGGGAGGCTTGTTGGGAAGGTAAAAGCGGATGTGGAGGCAATTTCCCTCATCAAAGGAATGGAGGTCAAGATGGAAGGCCCGTGCATGATCTCGACACTCATCTCACAGCAGTTGGGTATTAATTGTTGTGTTCTGATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGCGAAGCCACGGTTGGATACAGAGAGAACAAAGAGATTGCACAGAAATGGGTTGAGCTCTTTAGTACTTCCTACTTCATAGTCACACCTATCCAAGATGTGGAAGGGGTAGAACTATGTGGAACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGATGGGTTGGAGATGGGAAATAACACAAAGGCTGCAATTATGAGAATCGGTCTAAGAGAGATGAAGGCATTTTCCAAGATGTTATTTTCATCTGTCAAGGACACCACCTTCTTTGAAAGCTGCGGTGTTGCTGATCTCATCACGACATGCCTGGGAGGAAGAAACAGGAAAGTTGCGGAAGCTTTTGCAAGGAGTGGAGGGAAAAGATCGTTCGATGAGCTTGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTCTCAACAGCAAAAGAGGTTTACGAGGTTCTAAGCCACCGCGGGTGGCTAGATTTCTTCCCACTTTTTGCAACAGTTCATGAGATCTGCATTGGCACTCTTCCTCCATCAGCCATAGTTGAACACAGTGAGCGCACGCCTAAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

461

Amino Acids

50.36

Weight (kDa)

5.74

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 115 - 282 2.4e-40 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 304 - 452 2.1e-45 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 528, 956
AccB7I CCANNNNNTGG 1 cut(s) 913
AccII CGCG 2 cut(s) 218, 1275
AciI CCGC 9 cut(s) 131, 381, 496, 636, 662, 1098, 1151, 1273, 1275
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 1 cut(s) 809
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 1 cut(s) 880
AfiI CCNNNNNNNGG 2 cut(s) 298, 913
AflIII ACRYGT 3 cut(s) 116, 216, 591
AgsI TTSAA 4 cut(s) 86, 1091, 1199, 1355
AjnI CCWGG 1 cut(s) 1125
AjuI GAANNNNNNNTTGG 2 cut(s) 810, 842
AluBI AGCT 8 cut(s) 326, 478, 535, 788, 871, 1095, 1157, 1195
AluI AGCT 8 cut(s) 326, 478, 535, 788, 871, 1095, 1157, 1195
Alw21I GWGCWC 1 cut(s) 873
Alw26I GTCTC 2 cut(s) 171, 1240
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 3 cut(s) 316, 712, 954
ApeKI GCWGC 4 cut(s) 962, 1007, 1095, 1210
ApoI RAATTY 1 cut(s) 809
ArsI GACNNNNNNTTYG 2 cut(s) 327, 359
AseI ATTAAT 1 cut(s) 756
AspLEI GCGC 1 cut(s) 1368
AspS9I GGNCC 2 cut(s) 317, 713
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 337, 482
AvaI CYCGRG 1 cut(s) 407
BalI TGGCCA 1 cut(s) 956
BanII GRGCYC 2 cut(s) 414, 873
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 873
BbvI GCAGC 4 cut(s) 974, 994, 1082, 1197
BccI CCATC 6 cut(s) 497, 700, 766, 971, 982, 1348
BciT130I CCWGG 1 cut(s) 1127
BciVI GTATCC 1 cut(s) 824
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 171, 1240
BfaI CTAG 2 cut(s) 1283, 1381
BfmI CTRYAG 1 cut(s) 1211
BfuAI ACCTGC 2 cut(s) 528, 956
BfuI GTATCC 1 cut(s) 824
BglII AGATCT 1 cut(s) 1317
BisI GCNGC 5 cut(s) 381, 963, 1008, 1096, 1211
BlsI GCNGC 5 cut(s) 382, 964, 1009, 1097, 1212
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 2 cut(s) 525, 1127
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 317, 713
BmiI GGNNCC 2 cut(s) 282, 937
BmrFI CCNGG 2 cut(s) 525, 1127
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 2 cut(s) 108, 475
BmuI ACTGGG 1 cut(s) 376
BoxI GACNNNNGTC 1 cut(s) 142
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 587
BpuMI CCSGG 1 cut(s) 525
BsaJI CCNNGG 5 cut(s) 276, 406, 822, 1126, 1273
BsaXI ACNNNNNCTCC 2 cut(s) 1321, 1351
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 913
Bse1I ACTGG 2 cut(s) 319, 371
Bse3DI GCAATG 1 cut(s) 390
BseBI CCWGG 1 cut(s) 1127
BseDI CCNNGG 5 cut(s) 276, 406, 822, 1126, 1273
BseGI GGATG 3 cut(s) 272, 670, 982
BseLI CCNNNNNNNGG 2 cut(s) 298, 913
BseMI GCAATG 1 cut(s) 390
BseNI ACTGG 2 cut(s) 319, 371
BseRI GAGGAG 6 cut(s) 72, 159, 178, 225, 256, 318
BseXI GCAGC 4 cut(s) 974, 994, 1082, 1197
BseYI CCCAGC 1 cut(s) 535
Bsh1236I CGCG 2 cut(s) 218, 1275
BshFI GGCC 3 cut(s) 318, 714, 956
BsiHKAI GWGCWC 1 cut(s) 873
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 1 cut(s) 525
BslI CCNNNNNNNGG 2 cut(s) 298, 913
BsmAI GTCTC 2 cut(s) 171, 1240
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 3 cut(s) 318, 714, 956
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 414, 873
Bsp143I GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 9 cut(s) 131, 381, 496, 636, 662, 1098, 1151, 1273, 1275
BspANI GGCC 3 cut(s) 318, 714, 956
BspFNI CGCG 2 cut(s) 218, 1275
BspHI TCATGA 1 cut(s) 1312
BspLI GGNNCC 2 cut(s) 282, 937
BspMAI CTGCAG 1 cut(s) 1215
BspMI ACCTGC 2 cut(s) 528, 956
BsrDI GCAATG 1 cut(s) 390
BsrI ACTGG 2 cut(s) 319, 371
BssECI CCNNGG 5 cut(s) 276, 406, 822, 1126, 1273
BssMI GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 1127
Bst4CI ACNGT 5 cut(s) 349, 584, 826, 1309, 1361
Bst6I CTCTTC 3 cut(s) 274, 421, 1338
BstC8I GCNNGC 1 cut(s) 1370
BstDEI CTNAG 2 cut(s) 1028, 1265
BstDSI CCRYGG 3 cut(s) 276, 822, 1273
BstF5I GGATG 3 cut(s) 272, 670, 982
BstFNI CGCG 2 cut(s) 218, 1275
BstHHI GCGC 1 cut(s) 1368
BstKTI GATC 6 cut(s) 52, 64, 726, 1111, 1184, 1320
BstMAI GTCTC 2 cut(s) 171, 1240
BstMBI GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
BstMWI GCNNNNNNNGC 2 cut(s) 785, 962
BstNI CCWGG 1 cut(s) 1127
BstNSI RCATGY 2 cut(s) 595, 1125
BstPAI GACNNNNGTC 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 523, 1125
BstSFI CTRYAG 1 cut(s) 1211
BstUI CGCG 2 cut(s) 218, 1275
BstV1I GCAGC 4 cut(s) 974, 994, 1082, 1197
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 1317
BstYI RGATCY 1 cut(s) 1317
BsuI GTATCC 1 cut(s) 824
BsuRI GGCC 3 cut(s) 318, 714, 956
BtgI CCRYGG 3 cut(s) 276, 822, 1273
BtsCI GGATG 3 cut(s) 272, 670, 982
BtsI GCAGTG 2 cut(s) 364, 582
BtsIMutI CAGTG 3 cut(s) 364, 582, 1366
BveI ACCTGC 2 cut(s) 528, 956
Cac8I GCNNGC 1 cut(s) 1370
CciI TCATGA 1 cut(s) 1312
CfoI GCGC 1 cut(s) 1368
Cfr13I GGNCC 2 cut(s) 317, 713
Cfr42I CCGCGG 1 cut(s) 1276
Csp6I GTAC 1 cut(s) 879
CspCI CAANNNNNGTGG 2 cut(s) 1069, 1104
CviAII CATG 5 cut(s) 277, 592, 721, 1122, 1313
CviQI GTAC 1 cut(s) 879
DdeI CTNAG 2 cut(s) 1028, 1265
DpnI GATC 6 cut(s) 51, 63, 725, 1110, 1183, 1319
DpnII GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
EaeI YGGCCR 1 cut(s) 954
Eam1104I CTCTTC 3 cut(s) 274, 421, 1338
EarI CTCTTC 3 cut(s) 274, 421, 1338
EciI GGCGGA 1 cut(s) 120
Ecl136II GAGCTC 1 cut(s) 871
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 2 cut(s) 414, 873
Eco53kI GAGCTC 1 cut(s) 871
Eco57I CTGAAG 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 871
EcoRII CCWGG 1 cut(s) 1125
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 2 cut(s) 414, 873
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 5 cut(s) 280, 595, 724, 1125, 1316
FatI CATG 5 cut(s) 276, 591, 720, 1121, 1312
FauI CCCGC 2 cut(s) 629, 1268
Fnu4HI GCNGC 5 cut(s) 381, 963, 1008, 1096, 1211
FokI GGATG 3 cut(s) 259, 677, 989
FriOI GRGCYC 2 cut(s) 414, 873
Fsp4HI GCNGC 5 cut(s) 381, 963, 1008, 1096, 1211
FspBI CTAG 2 cut(s) 1283, 1381
GlaI GCGC 1 cut(s) 1367
GluI GCNGC 5 cut(s) 381, 963, 1008, 1096, 1211
GsaI CCCAGC 1 cut(s) 539
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 3 cut(s) 318, 714, 956
HapII CCGG 1 cut(s) 525
HhaI GCGC 1 cut(s) 1368
Hin1II CATG 5 cut(s) 280, 595, 724, 1125, 1316
Hin6I GCGC 1 cut(s) 1366
HinP1I GCGC 1 cut(s) 1366
HindIII AAGCTT 1 cut(s) 1155
HinfI GANTC 2 cut(s) 342, 1020
HpaII CCGG 1 cut(s) 525
HphI GGTGA 2 cut(s) 337, 482
Hpy166II GTNNAC 2 cut(s) 580, 1255
Hpy188I TCNGA 1 cut(s) 771
Hpy188III TCNNGA 5 cut(s) 284, 703, 727, 1117, 1313
Hpy8I GTNNAC 2 cut(s) 580, 1255
Hpy99I CGWCG 3 cut(s) 141, 353, 356
HpyAV CCTTC 7 cut(s) 185, 430, 646, 704, 911, 1033, 1093
HpyCH4III ACNGT 5 cut(s) 349, 584, 826, 1309, 1361
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 9 cut(s) 554, 575, 720, 854, 1010, 1163, 1213, 1304, 1323
HpyF10VI GCNNNNNNNGC 2 cut(s) 785, 962
HpyF3I CTNAG 2 cut(s) 1028, 1265
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 5 cut(s) 280, 595, 724, 1125, 1316
HspAI GCGC 1 cut(s) 1366
KspI CCGCGG 1 cut(s) 1276
Kzo9I GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
LmnI GCTCC 4 cut(s) 191, 286, 331, 776
Lsp1109I GCAGC 4 cut(s) 974, 994, 1082, 1197
LweI GCATC 2 cut(s) 108, 475
MaeI CTAG 2 cut(s) 1283, 1381
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 4 cut(s) 107, 343, 604, 895
MalI GATC 6 cut(s) 51, 63, 725, 1110, 1183, 1319
MboI GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
MboII GAAGA 7 cut(s) 138, 261, 314, 408, 1146, 1282, 1325
MflI RGATCY 1 cut(s) 1317
MhlI GDGCHC 2 cut(s) 414, 873
MlsI TGGCCA 1 cut(s) 956
MluCI AATT 9 cut(s) 34, 40, 91, 617, 675, 757, 809, 1011, 1223
MluI ACGCGT 1 cut(s) 216
MluNI TGGCCA 1 cut(s) 956
MlyI GAGTC 1 cut(s) 351
MmeI TCCRAC 4 cut(s) 316, 423, 808, 963
Mox20I TGGCCA 1 cut(s) 956
MscI TGGCCA 1 cut(s) 956
MseI TTAA 3 cut(s) 39, 531, 756
MslI CAYNNNNRTG 1 cut(s) 859
Msp20I TGGCCA 1 cut(s) 956
MspA1I CMGCKG 1 cut(s) 1275
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 2 cut(s) 525, 1127
Mva1269I GAATGC 1 cut(s) 577
MvaI CCWGG 1 cut(s) 1127
MvnI CGCG 2 cut(s) 218, 1275
MwoI GCNNNNNNNGC 2 cut(s) 785, 962
NciI CCSGG 1 cut(s) 525
NcoI CCATGG 1 cut(s) 276
NdeII GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
NlaIII CATG 5 cut(s) 280, 595, 724, 1125, 1316
NlaIV GGNNCC 2 cut(s) 282, 937
NmuCI GTSAC 3 cut(s) 107, 343, 895
NspI RCATGY 2 cut(s) 595, 1125
PagI TCATGA 1 cut(s) 1312
PciI ACATGT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 577
PfeI GAWTC 1 cut(s) 1020
PflMI CCANNNNNTGG 1 cut(s) 913
PkrI GCNGC 5 cut(s) 382, 964, 1009, 1097, 1212
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
PscI ACATGT 1 cut(s) 591
PshAI GACNNNNGTC 1 cut(s) 142
PshBI ATTAAT 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 873
Psp6I CCWGG 1 cut(s) 1125
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 1 cut(s) 1125
PspN4I GGNNCC 2 cut(s) 282, 937
PspPI GGNCC 2 cut(s) 317, 713
PstI CTGCAG 1 cut(s) 1215
PsuI RGATCY 1 cut(s) 1317
RsaI GTAC 1 cut(s) 880
RsaNI GTAC 1 cut(s) 879
RseI CAYNNNNRTG 1 cut(s) 859
SacI GAGCTC 1 cut(s) 873
SacII CCGCGG 1 cut(s) 1276
SaqAI TTAA 3 cut(s) 39, 531, 756
SatI GCNGC 5 cut(s) 381, 963, 1008, 1096, 1211
Sau3AI GATC 6 cut(s) 49, 61, 723, 1108, 1181, 1317
Sau96I GGNCC 2 cut(s) 317, 713
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 1 cut(s) 351
ScrFI CCNGG 2 cut(s) 525, 1127
SduI GDGCHC 2 cut(s) 414, 873
SfaNI GCATC 2 cut(s) 108, 475
SfcI CTRYAG 1 cut(s) 1211
Sfr303I CCGCGG 1 cut(s) 1276
SgrBI CCGCGG 1 cut(s) 1276
SmiMI CAYNNNNRTG 1 cut(s) 859
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 9 cut(s) 34, 40, 91, 617, 675, 757, 809, 1011, 1223
SsiI CCGC 9 cut(s) 131, 381, 496, 636, 662, 1098, 1151, 1273, 1275
SspMI CTAG 2 cut(s) 1283, 1381
SstI GAGCTC 1 cut(s) 873
StyD4I CCNGG 2 cut(s) 523, 1125
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 5 cut(s) 349, 584, 826, 1309, 1361
TaiI ACGT 1 cut(s) 119
TaqI TCGA 2 cut(s) 728, 1188
TaqII GACCGA 1 cut(s) 1013
TasI AATT 9 cut(s) 34, 40, 91, 617, 675, 757, 809, 1011, 1223
TatI WGTACW 1 cut(s) 878
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 1 cut(s) 1020
Tru1I TTAA 3 cut(s) 39, 531, 756
Tru9I TTAA 3 cut(s) 39, 531, 756
TscAI CASTG 3 cut(s) 364, 582, 1366
TseFI GTSAC 3 cut(s) 107, 343, 895
TseI GCWGC 4 cut(s) 962, 1007, 1095, 1210
Tsp45I GTSAC 3 cut(s) 107, 343, 895
TspDTI ATGAA 8 cut(s) 17, 261, 444, 519, 880, 1052, 1053, 1301
TspRI CASTG 3 cut(s) 364, 582, 1366
Van91I CCANNNNNTGG 1 cut(s) 913
VspI ATTAAT 1 cut(s) 756
XapI RAATTY 1 cut(s) 809
XceI RCATGY 2 cut(s) 595, 1125
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 2 cut(s) 1283, 1381
ZrmI AGTACT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.