RLG00000022206

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014375
Physical Location & Seq
Reverse (-)
81697208 .. 81699774
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022206

Sequence Viewer

Length: 1116 bp
ATGGCTCCACAGGAAGCAGCAGCTAGTGTGGCCCAGAAGAGCTCCTCCATTGATGAAGCTGCTCCTCTAAGAAACAGAGTCACAGTTGTGGGCAGTGGCAACTGGGGTAGTGTGGCTGCTAAGCTCATTGCCTCCAACACCATTAGGCTTCCTACTTTTCATGATGAAGTGAGAATGTGGGTGTTTGAGGAGACATTGCCAAGTGGGGAGAAGCTCACAGATGCCATCAACACAAGAAATGAAAATGTAAAATATCTGCCTGGCATAAAACTTGGGAGAAATGTTGTTGCAGACCCAGACCTTGTTAATGCAGTGACTGGTTCAAACATGTTGGTGTTTGTTACTCCACATCAATTCATGGAGGGTATATGCAAAAGACTAGCGGGGAAGATAAGTGGAGATGTGGAGGCTATTTCCCTTATTAAAGGAATGGAGGTCAAGATGGAAGGCCCATGCATGATCTCTACACTCATCACTGAGCAACTGGGTATCAATTGTTGTGTTCTAATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGTGAAGCCACAGTTGGATACAGAGAAATCAAAGAGGTTGCAGAGAAATGGGTTCAACTGTTTAGTACCTCCTACTTCATAGTCACACCTATCCAAGATGTGGAAGGCGTAGAACTGTGTGGTACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGATGGCTTGGACATGGGAAATAACACAAAGGCTGCAATTATGAGAATTGGTCTAAGAGAGATGAAGGCATTTTCCAAAATGTTATTTTCATCTGTCAAGGACAGCACCTTCTTTGAAAGCTGCGGTGTTGCTGATCTCATCACAACATGCTTGGGAGGAAGAAACAGGAAAGTTGCAGAAGCTTTCGCAAAGAATGGAGGGAAAAGATCGTTCGACGAGCTTGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTGTCAACAGCAAAAGAGGTTTATGCAGTTTTAAGCCACCGTGGGTGGTTACAGTTCTTTCCGCTTTTTGCAACAGTTCATGAGATCTGCATTGGCACTCTTCCTCCATCAGCCATAGTCGAACACAGTGAGCGCACACCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

372

Amino Acids

40.23

Weight (kDa)

5.87

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 27 - 192 1.4e-40 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 216 - 364 2.1e-45 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 692
Acc65I GGTACC 1 cut(s) 671
AccB1I GGYRCC 1 cut(s) 671
AccB7I CCANNNNNTGG 1 cut(s) 649
AciI CCGC 3 cut(s) 383, 834, 1031
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 1 cut(s) 545
AfaI GTAC 2 cut(s) 616, 673
AfiI CCNNNNNNNGG 1 cut(s) 649
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 4 cut(s) 324, 605, 827, 935
AjnI CCWGG 1 cut(s) 259
AjuI GAANNNNNNNTTGG 2 cut(s) 546, 578
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 9 cut(s) 23, 42, 59, 124, 214, 524, 831, 893, 931
AluI AGCT 9 cut(s) 23, 42, 59, 124, 214, 524, 831, 893, 931
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 185
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 3 cut(s) 30, 448, 690
ApeKI GCWGC 8 cut(s) 17, 20, 59, 116, 698, 743, 831, 946
ApoI RAATTY 1 cut(s) 545
Asp718I GGTACC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 1104
AspS9I GGNCC 2 cut(s) 31, 449
BalI TGGCCA 1 cut(s) 692
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 8 cut(s) 29, 32, 46, 103, 710, 730, 818, 933
BccI CCATC 4 cut(s) 233, 436, 707, 1084
BciT130I CCWGG 1 cut(s) 261
BciVI GTATCC 1 cut(s) 560
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 2 cut(s) 24, 380
BfmI CTRYAG 1 cut(s) 947
BfuAI ACCTGC 1 cut(s) 692
BfuI GTATCC 1 cut(s) 560
BglII AGATCT 1 cut(s) 1053
BisI GCNGC 8 cut(s) 18, 21, 60, 117, 699, 744, 832, 947
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 8 cut(s) 19, 22, 61, 118, 700, 745, 833, 948
Bme1390I CCNGG 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 31, 449
BmiI GGNNCC 2 cut(s) 6, 673
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 2 cut(s) 112, 494
BmsI GCATC 1 cut(s) 211
BmuI ACTGGG 2 cut(s) 112, 494
Bpu1102I GCTNAGC 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 1009
BsaXI ACNNNNNCTCC 2 cut(s) 1057, 1087
Bsc4I CCNNNNNNNGG 1 cut(s) 649
Bse1I ACTGG 3 cut(s) 107, 322, 489
Bse3DI GCAATG 2 cut(s) 126, 194
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 1009
BseGI GGATG 1 cut(s) 718
BseLI CCNNNNNNNGG 1 cut(s) 649
BseMI GCAATG 2 cut(s) 126, 194
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 3 cut(s) 107, 322, 489
BseRI GAGGAG 3 cut(s) 34, 54, 203
BseXI GCAGC 8 cut(s) 29, 32, 46, 103, 710, 730, 818, 933
BshFI GGCC 3 cut(s) 32, 450, 692
BshNI GGYRCC 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 649
BsmAI GTCTC 1 cut(s) 185
BsnI GGCC 3 cut(s) 32, 450, 692
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 4 cut(s) 459, 844, 917, 1053
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 3 cut(s) 383, 834, 1031
BspANI GGCC 3 cut(s) 32, 450, 692
BspCNI CTCAG 1 cut(s) 469
BspHI TCATGA 2 cut(s) 160, 1048
BspLI GGNNCC 2 cut(s) 6, 673
BspMAI CTGCAG 1 cut(s) 951
BspMI ACCTGC 1 cut(s) 692
BspQI GCTCTTC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 671
BsrDI GCAATG 2 cut(s) 126, 194
BsrI ACTGG 3 cut(s) 107, 322, 489
BssECI CCNNGG 1 cut(s) 1009
BssMI GATC 4 cut(s) 459, 844, 917, 1053
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 8 cut(s) 85, 562, 609, 666, 1010, 1023, 1045, 1097
Bst6I CTCTTC 2 cut(s) 32, 1074
BstDEI CTNAG 4 cut(s) 68, 120, 477, 764
BstDSI CCRYGG 1 cut(s) 1009
BstF5I GGATG 1 cut(s) 718
BstHHI GCGC 1 cut(s) 1104
BstKTI GATC 4 cut(s) 462, 847, 920, 1056
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 4 cut(s) 459, 844, 917, 1053
BstMWI GCNNNNNNNGC 3 cut(s) 29, 521, 698
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 2 cut(s) 331, 861
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 947
BstV1I GCAGC 8 cut(s) 29, 32, 46, 103, 710, 730, 818, 933
BstX2I RGATCY 1 cut(s) 1053
BstYI RGATCY 1 cut(s) 1053
BsuI GTATCC 1 cut(s) 560
BsuRI GGCC 3 cut(s) 32, 450, 692
BtgI CCRYGG 1 cut(s) 1009
BtsCI GGATG 1 cut(s) 718
BtsI GCAGTG 2 cut(s) 100, 318
BtsIMutI CAGTG 4 cut(s) 100, 318, 474, 1102
BveI ACCTGC 1 cut(s) 692
CaiI CAGNNNCTG 1 cut(s) 317
CciI TCATGA 2 cut(s) 160, 1048
CfoI GCGC 1 cut(s) 1104
Cfr13I GGNCC 2 cut(s) 31, 449
Csp6I GTAC 2 cut(s) 615, 672
CviAII CATG 8 cut(s) 161, 328, 358, 453, 457, 724, 858, 1049
CviQI GTAC 2 cut(s) 615, 672
DdeI CTNAG 4 cut(s) 68, 120, 477, 764
DpnI GATC 4 cut(s) 461, 846, 919, 1055
DpnII GATC 4 cut(s) 459, 844, 917, 1053
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 2 cut(s) 32, 1074
EarI CTCTTC 2 cut(s) 32, 1074
Ecl136II GAGCTC 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 259
EcoT22I ATGCAT 1 cut(s) 458
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 8 cut(s) 164, 331, 361, 456, 460, 727, 861, 1052
FatI CATG 8 cut(s) 160, 327, 357, 452, 456, 723, 857, 1048
FauI CCCGC 1 cut(s) 376
Fnu4HI GCNGC 8 cut(s) 18, 21, 60, 117, 699, 744, 832, 947
FokI GGATG 1 cut(s) 725
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 8 cut(s) 18, 21, 60, 117, 699, 744, 832, 947
FspBI CTAG 2 cut(s) 24, 380
GlaI GCGC 1 cut(s) 1103
GluI GCNGC 8 cut(s) 18, 21, 60, 117, 699, 744, 832, 947
HaeIII GGCC 3 cut(s) 32, 450, 692
HhaI GCGC 1 cut(s) 1104
Hin1II CATG 8 cut(s) 164, 331, 361, 456, 460, 727, 861, 1052
Hin6I GCGC 1 cut(s) 1102
HinP1I GCGC 1 cut(s) 1102
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HindIII AAGCTT 1 cut(s) 891
HinfI GANTC 1 cut(s) 78
Hpy166II GTNNAC 1 cut(s) 975
Hpy188III TCNNGA 3 cut(s) 161, 439, 1049
Hpy8I GTNNAC 1 cut(s) 975
Hpy99I CGWCG 1 cut(s) 929
HpyAV CCTTC 4 cut(s) 440, 647, 769, 829
HpyCH4III ACNGT 8 cut(s) 85, 562, 609, 666, 1010, 1023, 1045, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 521, 698
HpyF3I CTNAG 4 cut(s) 68, 120, 477, 764
Hsp92II CATG 8 cut(s) 164, 331, 361, 456, 460, 727, 861, 1052
HspAI GCGC 1 cut(s) 1102
KpnI GGTACC 1 cut(s) 675
Kzo9I GATC 4 cut(s) 459, 844, 917, 1053
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 4 cut(s) 10, 47, 67, 512
Lsp1109I GCAGC 8 cut(s) 29, 32, 46, 103, 710, 730, 818, 933
LweI GCATC 1 cut(s) 211
MaeI CTAG 2 cut(s) 24, 380
MaeIII GTNAC 5 cut(s) 79, 313, 340, 631, 1017
MalI GATC 4 cut(s) 461, 846, 919, 1055
MboI GATC 4 cut(s) 459, 844, 917, 1053
MboII GAAGA 4 cut(s) 49, 400, 882, 1061
MfeI CAATTG 1 cut(s) 493
MflI RGATCY 1 cut(s) 1053
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 6 cut(s) 353, 493, 545, 747, 756, 959
MluNI TGGCCA 1 cut(s) 692
MlyI GAGTC 1 cut(s) 87
MmeI TCCRAC 2 cut(s) 159, 544
Mox20I TGGCCA 1 cut(s) 692
Mph1103I ATGCAT 1 cut(s) 458
MscI TGGCCA 1 cut(s) 692
MseI TTAA 3 cut(s) 306, 423, 1001
MslI CAYNNNNRTG 2 cut(s) 86, 332
Msp20I TGGCCA 1 cut(s) 692
MspR9I CCNGG 1 cut(s) 261
MunI CAATTG 1 cut(s) 493
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 3 cut(s) 29, 521, 698
NdeII GATC 4 cut(s) 459, 844, 917, 1053
NlaIII CATG 8 cut(s) 164, 331, 361, 456, 460, 727, 861, 1052
NlaIV GGNNCC 2 cut(s) 6, 673
NmuCI GTSAC 3 cut(s) 79, 313, 631
NsiI ATGCAT 1 cut(s) 458
NspI RCATGY 2 cut(s) 331, 861
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 2 cut(s) 160, 1048
PciI ACATGT 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 649
PkrI GCNGC 8 cut(s) 19, 22, 61, 118, 700, 745, 833, 948
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PscI ACATGT 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 6, 673
PspPI GGNCC 2 cut(s) 31, 449
PstI CTGCAG 1 cut(s) 951
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 1 cut(s) 1053
RsaI GTAC 2 cut(s) 616, 673
RsaNI GTAC 2 cut(s) 615, 672
RseI CAYNNNNRTG 2 cut(s) 86, 332
SacI GAGCTC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 3 cut(s) 306, 423, 1001
SatI GCNGC 8 cut(s) 18, 21, 60, 117, 699, 744, 832, 947
Sau3AI GATC 4 cut(s) 459, 844, 917, 1053
Sau96I GGNCC 2 cut(s) 31, 449
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 1 cut(s) 211
SfcI CTRYAG 1 cut(s) 947
SmiMI CAYNNNNRTG 2 cut(s) 86, 332
Sse9I AATT 6 cut(s) 353, 493, 545, 747, 756, 959
SsiI CCGC 3 cut(s) 383, 834, 1031
SspMI CTAG 2 cut(s) 24, 380
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 259
TaaI ACNGT 8 cut(s) 85, 562, 609, 666, 1010, 1023, 1045, 1097
TaqI TCGA 2 cut(s) 924, 1089
TasI AATT 6 cut(s) 353, 493, 545, 747, 756, 959
Tru1I TTAA 3 cut(s) 306, 423, 1001
Tru9I TTAA 3 cut(s) 306, 423, 1001
TscAI CASTG 4 cut(s) 100, 318, 481, 1102
TseFI GTSAC 3 cut(s) 79, 313, 631
TseI GCWGC 8 cut(s) 17, 20, 59, 116, 698, 743, 831, 946
Tsp45I GTSAC 3 cut(s) 79, 313, 631
TspDTI ATGAA 9 cut(s) 69, 149, 180, 255, 346, 616, 788, 789, 1037
TspRI CASTG 4 cut(s) 100, 318, 481, 1102
Van91I CCANNNNNTGG 1 cut(s) 649
XapI RAATTY 1 cut(s) 545
XceI RCATGY 2 cut(s) 331, 861
XspI CTAG 2 cut(s) 24, 380
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.