Rroxscaffold_2G00078740

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014375
Physical Location & Seq
Forward (+)
1964836 .. 1967646
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078740.1

Sequence Viewer

Length: 1272 bp
ATGGCATCTCCGCCCCGACGAGTGTCTTCTCCCCTCATATATTCTCTCGCCTCTCTCCCTCTCTTCCACGCATTGCCCATTTACTCTTCTTCATTTCTCTCTCTCCTCCTCTCTCGCTCTCTCTCCTCTTCTTCTTCTTCTTCTTCATCATCATCAATGGCTCCACAGGAAGCAGCAGCTAGTGTGGCCCAGAAGAGCTCCTCCATTGATGAACCTGCTCCTCTAAGAAACAGAGTCACAGTCGTGGGCAGTGGCAACTGGGGTAGTGTGGCTGCTAAGCTCATTGCCTCCAACACCATTAGGCTTCCTTCTTTTCATGATGAAGTGAGAATGTGGGTGTTTGAGGAGACATTGCCAAGTGGTGAGAAGCTCACAGATGCCATCAACACAAGAAATGAAAATGTAAAATACCTGCCGGGCATAAAACTTGGGAGAAATGTTGTTGCAGACCCAGACCTTGTTAAAGCAGTGACTGGTTCAAACATGTTGGTGTTTGTTACTCCACATCAATTCATGGAGGGTATATGCAAAAGACTAGCGGGGAAGATAAGTGGGGATGTGGAGGCTATTTCCCTTATTAAAGGAATGGAGGTCAAGATGGAAGGCCCATGCATGATCTCTACACTCATCACTGAGCAACTGGGTATCAATTGTTGTGTTCTAATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGTGAAGCCACAGTTGGATACAGAGAAAACAAAGAGGTTGCAGAGAAATGGGTTCAACTGTTTAGTACCTCCTACTTCATAGTCACACCTATCCAAGACGTGGAAGGCGTAGAACTGTGTGGTACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGATGGCTTGGACATGGGAAATAACACAAAGGCTGCAATTATGAGAATTGGTCTAAGAGAGATGAAGGCATTTTCCAAAATGTTATTTTCATCTGTCAAGGACAGCACCTTCTTTGAAAGCTGCGGTGTTGCTGATCTCATCACAACATGCTTGGGAGGAAGAAACAGGAAAGTTGCAGAAGCTTTCGCAAAGAATGGAGGGAAAAGATCGTTCGATGAGCTTGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTGTCAACAGCAAAAGAGGTTTATGCAGTTTTAAGCCACCGTGGGTGGTTACAGTTCTTTCCGCTTTTTGCAACAGTTCATGAGATCTGCATTGGCACTCTTCCTCCATCAGCCATAGTTGAACACAGTGAGCGCACACCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

423

Amino Acids

45.69

Weight (kDa)

7.01

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 79 - 246 7.2e-41 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 268 - 416 2.7e-45 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 223, 420, 848
Acc65I GGTACC 1 cut(s) 827
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 805
AciI CCGC 4 cut(s) 11, 539, 990, 1187
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 1 cut(s) 701
AfaI GTAC 2 cut(s) 772, 829
AfiI CCNNNNNNNGG 1 cut(s) 805
AflIII ACRYGT 1 cut(s) 483
AgsI TTSAA 5 cut(s) 480, 761, 983, 1091, 1247
AjiI CACGTC 1 cut(s) 805
AjuI GAANNNNNNNTTGG 2 cut(s) 702, 734
AleI CACNNNNGTG 1 cut(s) 242
AluBI AGCT 8 cut(s) 179, 198, 280, 370, 680, 987, 1049, 1087
AluI AGCT 8 cut(s) 179, 198, 280, 370, 680, 987, 1049, 1087
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 341
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 3 cut(s) 186, 604, 846
ApeKI GCWGC 7 cut(s) 173, 176, 272, 854, 899, 987, 1102
ApoI RAATTY 1 cut(s) 701
Asp718I GGTACC 1 cut(s) 827
AspLEI GCGC 1 cut(s) 1260
AspS9I GGNCC 2 cut(s) 187, 605
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 1 cut(s) 374
BalI TGGCCA 1 cut(s) 848
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 7 cut(s) 185, 188, 259, 866, 886, 974, 1089
BccI CCATC 4 cut(s) 389, 592, 863, 1240
BciVI GTATCC 1 cut(s) 716
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 341
BfaI CTAG 2 cut(s) 180, 536
BfmI CTRYAG 1 cut(s) 1103
BfuAI ACCTGC 3 cut(s) 223, 420, 848
BfuI GTATCC 1 cut(s) 716
BglII AGATCT 1 cut(s) 1209
BisI GCNGC 7 cut(s) 174, 177, 273, 855, 900, 988, 1103
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 7 cut(s) 175, 178, 274, 856, 901, 989, 1104
Bme1390I CCNGG 1 cut(s) 417
BmgBI CACGTC 1 cut(s) 805
BmgT120I GGNCC 2 cut(s) 187, 605
BmiI GGNNCC 2 cut(s) 162, 829
BmrFI CCNGG 1 cut(s) 417
BmrI ACTGGG 2 cut(s) 268, 650
BmsI GCATC 2 cut(s) 14, 367
BmuI ACTGGG 2 cut(s) 268, 650
BoxI GACNNNNGTC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 18
Bpu1102I GCTNAGC 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 1165
BsaXI ACNNNNNCTCC 2 cut(s) 1213, 1243
Bsc4I CCNNNNNNNGG 1 cut(s) 805
Bse1I ACTGG 3 cut(s) 263, 478, 645
Bse3DI GCAATG 3 cut(s) 71, 282, 350
BseDI CCNNGG 1 cut(s) 1165
BseGI GGATG 2 cut(s) 562, 874
BseLI CCNNNNNNNGG 1 cut(s) 805
BseMI GCAATG 3 cut(s) 71, 282, 350
BseMII CTCAG 1 cut(s) 624
BseNI ACTGG 3 cut(s) 263, 478, 645
BseRI GAGGAG 6 cut(s) 95, 98, 115, 190, 210, 359
BseXI GCAGC 7 cut(s) 185, 188, 259, 866, 886, 974, 1089
BshFI GGCC 3 cut(s) 188, 606, 848
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 416
BslI CCNNNNNNNGG 1 cut(s) 805
BsmAI GTCTC 1 cut(s) 341
BsnI GGCC 3 cut(s) 188, 606, 848
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 4 cut(s) 615, 1000, 1073, 1209
Bsp1720I GCTNAGC 1 cut(s) 276
BspACI CCGC 4 cut(s) 11, 539, 990, 1187
BspANI GGCC 3 cut(s) 188, 606, 848
BspCNI CTCAG 1 cut(s) 625
BspHI TCATGA 2 cut(s) 316, 1204
BspLI GGNNCC 2 cut(s) 162, 829
BspMAI CTGCAG 1 cut(s) 1107
BspMI ACCTGC 3 cut(s) 223, 420, 848
BspQI GCTCTTC 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 827
BsrDI GCAATG 3 cut(s) 71, 282, 350
BsrI ACTGG 3 cut(s) 263, 478, 645
BssECI CCNNGG 1 cut(s) 1165
BssMI GATC 4 cut(s) 615, 1000, 1073, 1209
Bst4CI ACNGT 8 cut(s) 241, 718, 765, 822, 1166, 1179, 1201, 1253
Bst6I CTCTTC 5 cut(s) 68, 91, 133, 188, 1230
BstDEI CTNAG 4 cut(s) 224, 276, 633, 920
BstDSI CCRYGG 1 cut(s) 1165
BstF5I GGATG 2 cut(s) 562, 874
BstHHI GCGC 1 cut(s) 1260
BstKTI GATC 4 cut(s) 618, 1003, 1076, 1212
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 4 cut(s) 615, 1000, 1073, 1209
BstMWI GCNNNNNNNGC 3 cut(s) 185, 677, 854
BstNSI RCATGY 2 cut(s) 487, 1017
BstPAI GACNNNNGTC 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 415
BstSFI CTRYAG 1 cut(s) 1103
BstV1I GCAGC 7 cut(s) 185, 188, 259, 866, 886, 974, 1089
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 1209
BstYI RGATCY 1 cut(s) 1209
BsuI GTATCC 1 cut(s) 716
BsuRI GGCC 3 cut(s) 188, 606, 848
BtgI CCRYGG 1 cut(s) 1165
BtrI CACGTC 1 cut(s) 805
BtsCI GGATG 2 cut(s) 562, 874
BtsI GCAGTG 2 cut(s) 256, 474
BtsIMutI CAGTG 4 cut(s) 256, 474, 630, 1258
BveI ACCTGC 3 cut(s) 223, 420, 848
CaiI CAGNNNCTG 1 cut(s) 473
CciI TCATGA 2 cut(s) 316, 1204
CfoI GCGC 1 cut(s) 1260
Cfr13I GGNCC 2 cut(s) 187, 605
Csp6I GTAC 2 cut(s) 771, 828
CviAII CATG 8 cut(s) 317, 484, 514, 609, 613, 880, 1014, 1205
CviQI GTAC 2 cut(s) 771, 828
DdeI CTNAG 4 cut(s) 224, 276, 633, 920
DpnI GATC 4 cut(s) 617, 1002, 1075, 1211
DpnII GATC 4 cut(s) 615, 1000, 1073, 1209
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 5 cut(s) 68, 91, 133, 188, 1230
EarI CTCTTC 5 cut(s) 68, 91, 133, 188, 1230
Ecl136II GAGCTC 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 200
Eco53kI GAGCTC 1 cut(s) 198
EcoICRI GAGCTC 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 614
EcoT38I GRGCYC 1 cut(s) 200
FaeI CATG 8 cut(s) 320, 487, 517, 612, 616, 883, 1017, 1208
FatI CATG 8 cut(s) 316, 483, 513, 608, 612, 879, 1013, 1204
FauI CCCGC 1 cut(s) 532
Fnu4HI GCNGC 7 cut(s) 174, 177, 273, 855, 900, 988, 1103
FokI GGATG 2 cut(s) 569, 881
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 7 cut(s) 174, 177, 273, 855, 900, 988, 1103
FspBI CTAG 2 cut(s) 180, 536
GlaI GCGC 1 cut(s) 1259
GluI GCNGC 7 cut(s) 174, 177, 273, 855, 900, 988, 1103
HaeIII GGCC 3 cut(s) 188, 606, 848
HapII CCGG 1 cut(s) 416
HhaI GCGC 1 cut(s) 1260
Hin1II CATG 8 cut(s) 320, 487, 517, 612, 616, 883, 1017, 1208
Hin6I GCGC 1 cut(s) 1258
HinP1I GCGC 1 cut(s) 1258
HincII GTYRAC 1 cut(s) 1131
HindII GTYRAC 1 cut(s) 1131
HindIII AAGCTT 1 cut(s) 1047
HinfI GANTC 1 cut(s) 234
HpaII CCGG 1 cut(s) 416
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 1131
Hpy188III TCNNGA 3 cut(s) 317, 595, 1205
Hpy8I GTNNAC 1 cut(s) 1131
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 5 cut(s) 318, 596, 803, 925, 985
HpyCH4III ACNGT 8 cut(s) 241, 718, 765, 822, 1166, 1179, 1201, 1253
HpyCH4IV ACGT 1 cut(s) 804
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 677, 854
HpyF3I CTNAG 4 cut(s) 224, 276, 633, 920
HpySE526I ACGT 1 cut(s) 804
Hsp92II CATG 8 cut(s) 320, 487, 517, 612, 616, 883, 1017, 1208
HspAI GCGC 1 cut(s) 1258
KpnI GGTACC 1 cut(s) 831
Kzo9I GATC 4 cut(s) 615, 1000, 1073, 1209
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 4 cut(s) 166, 203, 223, 668
Lsp1109I GCAGC 7 cut(s) 185, 188, 259, 866, 886, 974, 1089
LweI GCATC 2 cut(s) 14, 367
MaeI CTAG 2 cut(s) 180, 536
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 5 cut(s) 235, 469, 496, 787, 1173
MalI GATC 4 cut(s) 617, 1002, 1075, 1211
MboI GATC 4 cut(s) 615, 1000, 1073, 1209
MfeI CAATTG 1 cut(s) 649
MflI RGATCY 1 cut(s) 1209
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 848
MluCI AATT 6 cut(s) 509, 649, 701, 903, 912, 1115
MluNI TGGCCA 1 cut(s) 848
MlyI GAGTC 1 cut(s) 243
MmeI TCCRAC 2 cut(s) 315, 700
Mox20I TGGCCA 1 cut(s) 848
Mph1103I ATGCAT 1 cut(s) 614
MscI TGGCCA 1 cut(s) 848
MseI TTAA 3 cut(s) 462, 579, 1157
MslI CAYNNNNRTG 2 cut(s) 242, 488
Msp20I TGGCCA 1 cut(s) 848
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 1 cut(s) 417
MunI CAATTG 1 cut(s) 649
MwoI GCNNNNNNNGC 3 cut(s) 185, 677, 854
NciI CCSGG 1 cut(s) 417
NdeII GATC 4 cut(s) 615, 1000, 1073, 1209
NlaIII CATG 8 cut(s) 320, 487, 517, 612, 616, 883, 1017, 1208
NlaIV GGNNCC 2 cut(s) 162, 829
NmuCI GTSAC 3 cut(s) 235, 469, 787
NsiI ATGCAT 1 cut(s) 614
NspI RCATGY 2 cut(s) 487, 1017
OliI CACNNNNGTG 1 cut(s) 242
PagI TCATGA 2 cut(s) 316, 1204
PciI ACATGT 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 1 cut(s) 810
PflMI CCANNNNNTGG 1 cut(s) 805
PkrI GCNGC 7 cut(s) 175, 178, 274, 856, 901, 989, 1104
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PscI ACATGT 1 cut(s) 483
PshAI GACNNNNGTC 1 cut(s) 22
Psp124BI GAGCTC 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 162, 829
PspPI GGNCC 2 cut(s) 187, 605
PstI CTGCAG 1 cut(s) 1107
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 1 cut(s) 1209
RsaI GTAC 2 cut(s) 772, 829
RsaNI GTAC 2 cut(s) 771, 828
RseI CAYNNNNRTG 2 cut(s) 242, 488
SacI GAGCTC 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 3 cut(s) 462, 579, 1157
SatI GCNGC 7 cut(s) 174, 177, 273, 855, 900, 988, 1103
Sau3AI GATC 4 cut(s) 615, 1000, 1073, 1209
Sau96I GGNCC 2 cut(s) 187, 605
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 200
SfaNI GCATC 2 cut(s) 14, 367
SfcI CTRYAG 1 cut(s) 1103
SmiMI CAYNNNNRTG 2 cut(s) 242, 488
Sse9I AATT 6 cut(s) 509, 649, 701, 903, 912, 1115
SsiI CCGC 4 cut(s) 11, 539, 990, 1187
SspMI CTAG 2 cut(s) 180, 536
SstI GAGCTC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 415
TaaI ACNGT 8 cut(s) 241, 718, 765, 822, 1166, 1179, 1201, 1253
TaiI ACGT 1 cut(s) 807
TaqI TCGA 1 cut(s) 1080
TasI AATT 6 cut(s) 509, 649, 701, 903, 912, 1115
Tru1I TTAA 3 cut(s) 462, 579, 1157
Tru9I TTAA 3 cut(s) 462, 579, 1157
TscAI CASTG 4 cut(s) 256, 474, 637, 1258
TseFI GTSAC 3 cut(s) 235, 469, 787
TseI GCWGC 7 cut(s) 173, 176, 272, 854, 899, 987, 1102
Tsp45I GTSAC 3 cut(s) 235, 469, 787
TspRI CASTG 4 cut(s) 256, 474, 637, 1258
Van91I CCANNNNNTGG 1 cut(s) 805
XapI RAATTY 1 cut(s) 701
XceI RCATGY 2 cut(s) 487, 1017
XspI CTAG 2 cut(s) 180, 536
Zsp2I ATGCAT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.