pycom111g01680

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0014375
Physical Location & Seq
Forward (+)
1039198 .. 1041476
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01680.1

Sequence Viewer

Length: 1107 bp
ATGGCTCCAGAACTTCAGGAGGAAAATTACTCTACTGGCTCTGCTGAACCTCCCCAGAAAACCAGAGTCACCGTCGTAGGCAGTGGCAACTGGGGCAGTGTTGCCGCCAAGCTCATTGCCTCCAACACCCGCAGGCTCTCTTCTTTCCACGATGAAGTAAGAATGTGGGTGTTTGAGGAAACGTTACCAAGTGGTGAGAAGCTCACAGATGCCATCAACCGCAACAATGAGAATGTGAAATATCTGCCTGGCATTAAGCTGGGGAAAAATGTTGTTGCAGACCCGGATCTTGAGAATGCAGTGAACGGTGCGAACATGTTGGTGTTTGTTACTCCACATCAATTTATGGAGGGTATATGCAGGAGGCTTGTTGGGAAGGTAAAAGCAGATGTGGAGGCGATTTCCCTCATCAAAGGAATGGAGGTCAAGATGGAAGGCCCATGCATGATCTCGACACTCATCTCAGATCAATTGGATATTAATTGTTGTGTTCTGATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGTGAAGCCACGGTTGGATACAGAGAAAAAAAAGAGATTGCACAGAAATGGGTTCAGCTGTTTAGTACTTCCTACTTCATAGTCACACCTGTCCAAGACGTGGAAGGAGTAGAACTGTGTGGAACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGACGGATTGGAGATGGGAAATAACACAAAGGCTGCAATTATGAGAATTGGTCTAAGGGAAATGAAGGCATTTTCCAAGATGTTATTCTCATCTGTCAAGGACACCACCTTCTTCGAAAGCTGTGGCGTTGCTGATCTCATCACAACATGCCTGGGAGGAAGAAACAGGAAAGTAGCGGAAGCTTTTGCAAGGAGTGAAGGGAAAAGATCGTTTGATGAGCTCGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTCTCAACCGCAAAAGAGGTTTACGAAGTTTTAAGCCACCGTGGGTGGCTAGATTTCTTCCCACTTTTTGCAACAGTTCATGAGATCTGCATTGGCACTCTTCCTCCAACAGCCATAGTTGAACACAGTGAGCGCACGCCTAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

369

Amino Acids

40.46

Weight (kDa)

5.5

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 23 - 190 4.3e-41 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 212 - 360 1.6e-45 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 680
AccB7I CCANNNNNTGG 1 cut(s) 637
AciI CCGC 5 cut(s) 105, 130, 220, 875, 966
AclI AACGTT 1 cut(s) 182
AclWI GGATC 1 cut(s) 294
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 1 cut(s) 533
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 1 cut(s) 637
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 1 cut(s) 1079
AjiI CACGTC 1 cut(s) 637
AjnI CCWGG 2 cut(s) 247, 849
AjuI GAANNNNNNNTTGG 4 cut(s) 534, 566, 767, 799
AluBI AGCT 8 cut(s) 112, 202, 259, 512, 595, 819, 881, 919
AluI AGCT 8 cut(s) 112, 202, 259, 512, 595, 819, 881, 919
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 1 cut(s) 964
AlwI GGATC 1 cut(s) 294
AoxI GGCC 2 cut(s) 436, 678
ApeKI GCWGC 3 cut(s) 686, 731, 934
ApoI RAATTY 1 cut(s) 533
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 1 cut(s) 1092
AspS9I GGNCC 1 cut(s) 437
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 2 cut(s) 61, 206
AsuII TTCGAA 1 cut(s) 813
BalI TGGCCA 1 cut(s) 680
BanII GRGCYC 1 cut(s) 921
Bbv12I GWGCWC 1 cut(s) 921
BbvI GCAGC 3 cut(s) 698, 718, 921
BccI CCATC 4 cut(s) 221, 424, 490, 706
BciT130I CCWGG 2 cut(s) 249, 851
BciVI GTATCC 1 cut(s) 548
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 964
BfaI CTAG 1 cut(s) 1007
BfmI CTRYAG 1 cut(s) 935
BfuAI ACCTGC 1 cut(s) 680
BfuI GTATCC 1 cut(s) 548
BglII AGATCT 1 cut(s) 1041
BisI GCNGC 4 cut(s) 105, 687, 732, 935
BlsI GCNGC 4 cut(s) 106, 688, 733, 936
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 3 cut(s) 249, 284, 851
BmgBI CACGTC 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 2 cut(s) 6, 661
BmrFI CCNGG 3 cut(s) 249, 284, 851
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 1 cut(s) 199
BmuI ACTGGG 1 cut(s) 100
Bpu14I TTCGAA 1 cut(s) 813
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 546, 850, 997
BsaXI ACNNNNNCTCC 2 cut(s) 1045, 1075
Bsc4I CCNNNNNNNGG 1 cut(s) 637
Bse1I ACTGG 2 cut(s) 40, 95
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 2 cut(s) 249, 851
BseDI CCNNGG 3 cut(s) 546, 850, 997
BseLI CCNNNNNNNGG 1 cut(s) 637
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 2 cut(s) 40, 95
BseXI GCAGC 3 cut(s) 698, 718, 921
BseYI CCCAGC 1 cut(s) 259
BshFI GGCC 2 cut(s) 438, 680
BsiHKAI GWGCWC 1 cut(s) 921
BsiSI CCGG 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 637
BsmAI GTCTC 1 cut(s) 964
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 2 cut(s) 438, 680
Bsp119I TTCGAA 1 cut(s) 813
Bsp1286I GDGCHC 1 cut(s) 921
Bsp143I GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
BspACI CCGC 5 cut(s) 105, 130, 220, 875, 966
BspANI GGCC 2 cut(s) 438, 680
BspCNI CTCAG 1 cut(s) 476
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 2 cut(s) 6, 661
BspMAI CTGCAG 1 cut(s) 939
BspMI ACCTGC 1 cut(s) 680
BspPI GGATC 1 cut(s) 294
BspT104I TTCGAA 1 cut(s) 813
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 2 cut(s) 40, 95
BssECI CCNNGG 3 cut(s) 546, 850, 997
BssMI GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
Bst2UI CCWGG 2 cut(s) 249, 851
Bst4CI ACNGT 7 cut(s) 73, 308, 550, 654, 998, 1033, 1085
Bst6I CTCTTC 2 cut(s) 145, 1062
BstBI TTCGAA 1 cut(s) 813
BstC8I GCNNGC 2 cut(s) 134, 1094
BstDEI CTNAG 2 cut(s) 463, 752
BstDSI CCRYGG 2 cut(s) 546, 997
BstHHI GCGC 1 cut(s) 1092
BstKTI GATC 6 cut(s) 289, 450, 469, 835, 908, 1044
BstMAI GTCTC 1 cut(s) 964
BstMBI GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
BstMWI GCNNNNNNNGC 3 cut(s) 93, 509, 686
BstNI CCWGG 2 cut(s) 249, 851
BstNSI RCATGY 2 cut(s) 319, 849
BstSCI CCNGG 3 cut(s) 247, 282, 849
BstSFI CTRYAG 1 cut(s) 935
BstV1I GCAGC 3 cut(s) 698, 718, 921
BstX2I RGATCY 2 cut(s) 286, 1041
BstYI RGATCY 2 cut(s) 286, 1041
BsuI GTATCC 1 cut(s) 548
BsuRI GGCC 2 cut(s) 438, 680
BtgI CCRYGG 2 cut(s) 546, 997
BtrI CACGTC 1 cut(s) 637
BtsI GCAGTG 3 cut(s) 88, 103, 306
BtsIMutI CAGTG 4 cut(s) 88, 103, 306, 1090
BveI ACCTGC 1 cut(s) 680
Cac8I GCNNGC 2 cut(s) 134, 1094
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 1 cut(s) 1092
Cfr13I GGNCC 1 cut(s) 437
Csp6I GTAC 1 cut(s) 603
CviAII CATG 5 cut(s) 316, 441, 445, 846, 1037
CviQI GTAC 1 cut(s) 603
DdeI CTNAG 2 cut(s) 463, 752
DpnI GATC 6 cut(s) 288, 449, 468, 834, 907, 1043
DpnII GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 2 cut(s) 145, 1062
EarI CTCTTC 2 cut(s) 145, 1062
Ecl136II GAGCTC 1 cut(s) 919
Eco24I GRGCYC 1 cut(s) 921
Eco53kI GAGCTC 1 cut(s) 919
EcoICRI GAGCTC 1 cut(s) 919
EcoRII CCWGG 2 cut(s) 247, 849
EcoT22I ATGCAT 1 cut(s) 446
EcoT38I GRGCYC 1 cut(s) 921
FaeI CATG 5 cut(s) 319, 444, 448, 849, 1040
FatI CATG 5 cut(s) 315, 440, 444, 845, 1036
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 4 cut(s) 105, 687, 732, 935
FriOI GRGCYC 1 cut(s) 921
Fsp4HI GCNGC 4 cut(s) 105, 687, 732, 935
FspBI CTAG 1 cut(s) 1007
GlaI GCGC 1 cut(s) 1091
GluI GCNGC 4 cut(s) 105, 687, 732, 935
GsaI CCCAGC 1 cut(s) 263
HaeIII GGCC 2 cut(s) 438, 680
HapII CCGG 1 cut(s) 284
HhaI GCGC 1 cut(s) 1092
Hin1II CATG 5 cut(s) 319, 444, 448, 849, 1040
Hin6I GCGC 1 cut(s) 1090
HinP1I GCGC 1 cut(s) 1090
HindIII AAGCTT 1 cut(s) 879
HinfI GANTC 1 cut(s) 66
HpaII CCGG 1 cut(s) 284
HphI GGTGA 2 cut(s) 61, 206
Hpy166II GTNNAC 3 cut(s) 304, 700, 979
Hpy188I TCNGA 2 cut(s) 466, 495
Hpy188III TCNNGA 6 cut(s) 8, 17, 290, 427, 451, 1037
Hpy8I GTNNAC 3 cut(s) 304, 700, 979
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 6 cut(s) 370, 428, 635, 757, 817, 890
HpyCH4III ACNGT 7 cut(s) 73, 308, 550, 654, 998, 1033, 1085
HpyCH4IV ACGT 2 cut(s) 182, 636
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 509, 686
HpyF3I CTNAG 2 cut(s) 463, 752
HpySE526I ACGT 2 cut(s) 182, 636
Hsp92II CATG 5 cut(s) 319, 444, 448, 849, 1040
HspAI GCGC 1 cut(s) 1090
Kzo9I GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
LmnI GCTCC 2 cut(s) 10, 500
Lsp1109I GCAGC 3 cut(s) 698, 718, 921
LweI GCATC 1 cut(s) 199
MaeI CTAG 1 cut(s) 1007
MaeII ACGT 2 cut(s) 182, 636
MaeIII GTNAC 4 cut(s) 67, 183, 328, 619
MalI GATC 6 cut(s) 288, 449, 468, 834, 907, 1043
MboI GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
MboII GAAGA 5 cut(s) 132, 802, 870, 1006, 1049
MfeI CAATTG 1 cut(s) 470
MflI RGATCY 2 cut(s) 286, 1041
MhlI GDGCHC 1 cut(s) 921
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 8 cut(s) 25, 341, 470, 481, 533, 735, 744, 947
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 3 cut(s) 147, 532, 1088
Mox20I TGGCCA 1 cut(s) 680
Mph1103I ATGCAT 1 cut(s) 446
MscI TGGCCA 1 cut(s) 680
MseI TTAA 3 cut(s) 255, 480, 989
MslI CAYNNNNRTG 2 cut(s) 320, 583
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 595
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 3 cut(s) 249, 284, 851
MunI CAATTG 1 cut(s) 470
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 2 cut(s) 249, 851
MwoI GCNNNNNNNGC 3 cut(s) 93, 509, 686
NciI CCSGG 1 cut(s) 284
NdeII GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
NlaIII CATG 5 cut(s) 319, 444, 448, 849, 1040
NlaIV GGNNCC 2 cut(s) 6, 661
NmuCI GTSAC 2 cut(s) 67, 619
NsiI ATGCAT 1 cut(s) 446
NspI RCATGY 2 cut(s) 319, 849
NspV TTCGAA 1 cut(s) 813
PagI TCATGA 1 cut(s) 1036
PciI ACATGT 1 cut(s) 315
PctI GAATGC 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 637
PkrI GCNGC 4 cut(s) 106, 688, 733, 936
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PscI ACATGT 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 921
Psp1406I AACGTT 1 cut(s) 182
Psp6I CCWGG 2 cut(s) 247, 849
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 2 cut(s) 247, 849
PspN4I GGNNCC 2 cut(s) 6, 661
PspPI GGNCC 1 cut(s) 437
PstI CTGCAG 1 cut(s) 939
PsuI RGATCY 2 cut(s) 286, 1041
PvuII CAGCTG 1 cut(s) 595
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
RseI CAYNNNNRTG 2 cut(s) 320, 583
SacI GAGCTC 1 cut(s) 921
SaqAI TTAA 3 cut(s) 255, 480, 989
SatI GCNGC 4 cut(s) 105, 687, 732, 935
Sau3AI GATC 6 cut(s) 286, 447, 466, 832, 905, 1041
Sau96I GGNCC 1 cut(s) 437
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 3 cut(s) 249, 284, 851
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 1 cut(s) 935
SfuI TTCGAA 1 cut(s) 813
SmiMI CAYNNNNRTG 2 cut(s) 320, 583
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 8 cut(s) 25, 341, 470, 481, 533, 735, 744, 947
SsiI CCGC 5 cut(s) 105, 130, 220, 875, 966
SspMI CTAG 1 cut(s) 1007
SstI GAGCTC 1 cut(s) 921
StyD4I CCNGG 3 cut(s) 247, 282, 849
TaaI ACNGT 7 cut(s) 73, 308, 550, 654, 998, 1033, 1085
TaiI ACGT 2 cut(s) 185, 639
TaqI TCGA 3 cut(s) 452, 813, 921
TasI AATT 8 cut(s) 25, 341, 470, 481, 533, 735, 744, 947
TatI WGTACW 1 cut(s) 602
TauI GCSGC 1 cut(s) 107
Tru1I TTAA 3 cut(s) 255, 480, 989
Tru9I TTAA 3 cut(s) 255, 480, 989
TscAI CASTG 4 cut(s) 88, 103, 306, 1090
TseFI GTSAC 2 cut(s) 67, 619
TseI GCWGC 3 cut(s) 686, 731, 934
Tsp45I GTSAC 2 cut(s) 67, 619
TspDTI ATGAA 4 cut(s) 168, 604, 776, 1025
TspGWI ACGGA 1 cut(s) 717
TspRI CASTG 4 cut(s) 88, 103, 306, 1090
Van91I CCANNNNNTGG 1 cut(s) 637
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 533
XceI RCATGY 2 cut(s) 319, 849
XspI CTAG 1 cut(s) 1007
ZrmI AGTACT 1 cut(s) 604
Zsp2I ATGCAT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.