Rw2G053400

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014375
Physical Location & Seq
Reverse (-)
81155131 .. 81157871
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053400.1

Sequence Viewer

Length: 1314 bp
ATGGCATCTCCGCCCCGACGAGTGTCTTCTCCCCTCTTATATTCTCTCGCCTCTCTCCCTCTCTTCCAGGCATTACCCATTTACCCTTCTTCATTTCTCTCTCTCCTCCTCTCTCACTCTCTCTCCTCTTCTTCTTCTTCCTCTTCATCAATGGCTCCACAGGAAGCAGCAGCTAGTGTGGCCCAGAAGAGCTCCTCCATTGATGAACCTGCTCCTCTAAGAAACAGAGTCACAGTTGTGGGCAGTGGCAACTGGGGTAGTGTGGCTGCTAAGCTCATTGCCTCCAACACCATTAGGCTTCCTTCTTTTCATGATGAAGTGAGAATGTGGGTGTTTGAGGAGACATTGCCAAGTGATGAGAAGCTCACAGATGCCATCAACACAAGAAATGAAAATGTAAAATATCTGCCTGGCATAAAACTTGGGAGAAATGTTGTTGCAGATCCAGACCTGTTAATGCAGGTACTCATTTTCTCAGCAAAAGCTTTGCTGACCTTGTCAAAGTTACTCTTGACTGGTTCAAACATGTTGGTGTTTGTTACTCCACATCAATTCATGGAGGGTATATGCAAAAGACTAGCGGGGAATATAAGCGGGGATGTGGAGGCTATTTCCCTTATTAAAGGAATGGAGGTCAAGATGGAAGGCCCATGCATGATCTCTACACTCATCACTGAGCAACTGGGTATCAATTGTTGTGTTCTAATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGTGAAGCCACTGTTGGATACAGAGAAAACAAAGACGTTGCAGAGAAATGGGTTCAACTGTTTAGTACCTCCTACTTCATAGTCACACCTATCCAAGACGTGGAAGGCGTAGAACTGTGTGGTACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGATGGCTTGGACATGGGAAATAACACAAAGGCTGCAATTATGAGAATTGGTCTAAGAGAGATGAAGGCATTTTCCAAAATGTTATTTTCATCTGTCAAGGACAGCACCTTCTTTGAAAGCTGCGGTGTTGCTGATCTCATCACAACATGCTTGGGAGGAAGAAACAGGAAAGTTGCAGAAGCTTTCGCAAAGAATGGAGGGAAAAGATCGTTCGATGAGCTTGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTGTCAACAGCAAAAGAGGTTTATGCAGTTTTAAGCCACCGTGGGTGGTTACAGTTCTTTCCGCTCTTTGCAACAGTTCATGAGATCTGCATTGGCACTCTTCCTCCATCAGCCATCGTCGAACACAGTGAGCGCACACCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

437

Amino Acids

47.32

Weight (kDa)

6.35

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 77 - 259 6.2e-33 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 282 - 430 2.9e-45 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 217, 451, 890
Acc65I GGTACC 1 cut(s) 869
AccB1I GGYRCC 1 cut(s) 869
AccB7I CCANNNNNTGG 1 cut(s) 847
AccBSI CCGCTC 1 cut(s) 1231
AciI CCGC 5 cut(s) 11, 581, 594, 1032, 1229
AclWI GGATC 1 cut(s) 437
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 1 cut(s) 743
AfaI GTAC 3 cut(s) 465, 814, 871
AfiI CCNNNNNNNGG 1 cut(s) 847
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 4 cut(s) 522, 803, 1025, 1133
AjiI CACGTC 1 cut(s) 847
AjnI CCWGG 2 cut(s) 66, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 744, 776
AleI CACNNNNGTG 1 cut(s) 236
AluBI AGCT 9 cut(s) 173, 192, 274, 364, 485, 722, 1029, 1091, 1129
AluI AGCT 9 cut(s) 173, 192, 274, 364, 485, 722, 1029, 1091, 1129
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 437
AoxI GGCC 3 cut(s) 180, 646, 888
ApeKI GCWGC 7 cut(s) 167, 170, 266, 896, 941, 1029, 1144
ApoI RAATTY 1 cut(s) 743
Asp718I GGTACC 1 cut(s) 869
AspLEI GCGC 1 cut(s) 1302
AspS9I GGNCC 2 cut(s) 181, 647
BalI TGGCCA 1 cut(s) 890
BanI GGYRCC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 7 cut(s) 179, 182, 253, 908, 928, 1016, 1131
BccI CCATC 5 cut(s) 383, 634, 905, 1282, 1289
BciT130I CCWGG 2 cut(s) 68, 411
BciVI GTATCC 1 cut(s) 758
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 2 cut(s) 174, 578
BfmI CTRYAG 1 cut(s) 1145
BfuAI ACCTGC 3 cut(s) 217, 451, 890
BfuI GTATCC 1 cut(s) 758
BglII AGATCT 1 cut(s) 1251
BisI GCNGC 7 cut(s) 168, 171, 267, 897, 942, 1030, 1145
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 7 cut(s) 169, 172, 268, 898, 943, 1031, 1146
Bme1390I CCNGG 2 cut(s) 68, 411
BmgBI CACGTC 1 cut(s) 847
BmgT120I GGNCC 2 cut(s) 181, 647
BmiI GGNNCC 2 cut(s) 156, 871
BmrFI CCNGG 2 cut(s) 68, 411
BmrI ACTGGG 2 cut(s) 262, 692
BmsI GCATC 2 cut(s) 14, 361
BmuI ACTGGG 2 cut(s) 262, 692
BoxI GACNNNNGTC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 18
Bpu1102I GCTNAGC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 1207
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 1255, 1285
Bsc4I CCNNNNNNNGG 1 cut(s) 847
Bse1I ACTGG 3 cut(s) 257, 520, 687
Bse3DI GCAATG 2 cut(s) 276, 344
BseBI CCWGG 2 cut(s) 68, 411
BseDI CCNNGG 1 cut(s) 1207
BseGI GGATG 2 cut(s) 604, 916
BseLI CCNNNNNNNGG 1 cut(s) 847
BseMI GCAATG 2 cut(s) 276, 344
BseMII CTCAG 2 cut(s) 489, 666
BseNI ACTGG 3 cut(s) 257, 520, 687
BseRI GAGGAG 6 cut(s) 95, 98, 115, 184, 204, 353
BseXI GCAGC 7 cut(s) 179, 182, 253, 908, 928, 1016, 1131
BshFI GGCC 3 cut(s) 182, 648, 890
BshNI GGYRCC 1 cut(s) 869
BsiHKAI GWGCWC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 847
BsmAI GTCTC 1 cut(s) 335
BsnI GGCC 3 cut(s) 182, 648, 890
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 5 cut(s) 442, 657, 1042, 1115, 1251
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 5 cut(s) 11, 581, 594, 1032, 1229
BspANI GGCC 3 cut(s) 182, 648, 890
BspCNI CTCAG 2 cut(s) 488, 667
BspHI TCATGA 2 cut(s) 310, 1246
BspLI GGNNCC 2 cut(s) 156, 871
BspMAI CTGCAG 1 cut(s) 1149
BspMI ACCTGC 3 cut(s) 217, 451, 890
BspPI GGATC 1 cut(s) 437
BspQI GCTCTTC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 869
BsrBI CCGCTC 1 cut(s) 1231
BsrDI GCAATG 2 cut(s) 276, 344
BsrI ACTGG 3 cut(s) 257, 520, 687
BssECI CCNNGG 1 cut(s) 1207
BssMI GATC 5 cut(s) 442, 657, 1042, 1115, 1251
Bst2UI CCWGG 2 cut(s) 68, 411
Bst4CI ACNGT 8 cut(s) 235, 760, 807, 864, 1208, 1221, 1243, 1295
Bst6I CTCTTC 5 cut(s) 68, 133, 148, 182, 1272
BstDEI CTNAG 5 cut(s) 218, 270, 475, 675, 962
BstDSI CCRYGG 1 cut(s) 1207
BstF5I GGATG 2 cut(s) 604, 916
BstHHI GCGC 1 cut(s) 1302
BstKTI GATC 5 cut(s) 445, 660, 1045, 1118, 1254
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 5 cut(s) 442, 657, 1042, 1115, 1251
BstMWI GCNNNNNNNGC 3 cut(s) 179, 719, 896
BstNI CCWGG 2 cut(s) 68, 411
BstNSI RCATGY 2 cut(s) 529, 1059
BstPAI GACNNNNGTC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 66, 409
BstSFI CTRYAG 1 cut(s) 1145
BstV1I GCAGC 7 cut(s) 179, 182, 253, 908, 928, 1016, 1131
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 2 cut(s) 442, 1251
BstYI RGATCY 2 cut(s) 442, 1251
BsuI GTATCC 1 cut(s) 758
BsuRI GGCC 3 cut(s) 182, 648, 890
BtgI CCRYGG 1 cut(s) 1207
BtrI CACGTC 1 cut(s) 847
BtsCI GGATG 2 cut(s) 604, 916
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 4 cut(s) 250, 672, 756, 1300
BveI ACCTGC 3 cut(s) 217, 451, 890
CciI TCATGA 2 cut(s) 310, 1246
CfoI GCGC 1 cut(s) 1302
Cfr13I GGNCC 2 cut(s) 181, 647
Csp6I GTAC 3 cut(s) 464, 813, 870
CviAII CATG 8 cut(s) 311, 526, 556, 651, 655, 922, 1056, 1247
CviQI GTAC 3 cut(s) 464, 813, 870
DdeI CTNAG 5 cut(s) 218, 270, 475, 675, 962
DpnI GATC 5 cut(s) 444, 659, 1044, 1117, 1253
DpnII GATC 5 cut(s) 442, 657, 1042, 1115, 1251
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 5 cut(s) 68, 133, 148, 182, 1272
EarI CTCTTC 5 cut(s) 68, 133, 148, 182, 1272
Ecl136II GAGCTC 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 192
EcoRII CCWGG 2 cut(s) 66, 409
EcoT22I ATGCAT 1 cut(s) 656
EcoT38I GRGCYC 1 cut(s) 194
FaeI CATG 8 cut(s) 314, 529, 559, 654, 658, 925, 1059, 1250
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FatI CATG 8 cut(s) 310, 525, 555, 650, 654, 921, 1055, 1246
FauI CCCGC 2 cut(s) 574, 587
Fnu4HI GCNGC 7 cut(s) 168, 171, 267, 897, 942, 1030, 1145
FokI GGATG 2 cut(s) 611, 923
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 7 cut(s) 168, 171, 267, 897, 942, 1030, 1145
FspBI CTAG 2 cut(s) 174, 578
GlaI GCGC 1 cut(s) 1301
GluI GCNGC 7 cut(s) 168, 171, 267, 897, 942, 1030, 1145
HaeIII GGCC 3 cut(s) 182, 648, 890
HhaI GCGC 1 cut(s) 1302
Hin1II CATG 8 cut(s) 314, 529, 559, 654, 658, 925, 1059, 1250
Hin6I GCGC 1 cut(s) 1300
HinP1I GCGC 1 cut(s) 1300
HincII GTYRAC 1 cut(s) 1173
HindII GTYRAC 1 cut(s) 1173
HindIII AAGCTT 2 cut(s) 483, 1089
HinfI GANTC 1 cut(s) 228
Hpy166II GTNNAC 1 cut(s) 1173
Hpy188III TCNNGA 5 cut(s) 311, 446, 511, 637, 1247
Hpy8I GTNNAC 1 cut(s) 1173
Hpy99I CGWCG 2 cut(s) 21, 1289
HpyAV CCTTC 6 cut(s) 96, 312, 638, 845, 967, 1027
HpyCH4III ACNGT 8 cut(s) 235, 760, 807, 864, 1208, 1221, 1243, 1295
HpyCH4IV ACGT 2 cut(s) 783, 846
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 719, 896
HpyF3I CTNAG 5 cut(s) 218, 270, 475, 675, 962
HpySE526I ACGT 2 cut(s) 783, 846
Hsp92II CATG 8 cut(s) 314, 529, 559, 654, 658, 925, 1059, 1250
HspAI GCGC 1 cut(s) 1300
KpnI GGTACC 1 cut(s) 873
Kzo9I GATC 5 cut(s) 442, 657, 1042, 1115, 1251
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 4 cut(s) 160, 197, 217, 710
Lsp1109I GCAGC 7 cut(s) 179, 182, 253, 908, 928, 1016, 1131
LweI GCATC 2 cut(s) 14, 361
MaeI CTAG 2 cut(s) 174, 578
MaeII ACGT 2 cut(s) 783, 846
MaeIII GTNAC 5 cut(s) 229, 504, 538, 829, 1215
MalI GATC 5 cut(s) 444, 659, 1044, 1117, 1253
MbiI CCGCTC 1 cut(s) 1231
MboI GATC 5 cut(s) 442, 657, 1042, 1115, 1251
MfeI CAATTG 1 cut(s) 691
MflI RGATCY 2 cut(s) 442, 1251
MhlI GDGCHC 1 cut(s) 194
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 6 cut(s) 551, 691, 743, 945, 954, 1157
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 2 cut(s) 309, 742
Mox20I TGGCCA 1 cut(s) 890
Mph1103I ATGCAT 1 cut(s) 656
MscI TGGCCA 1 cut(s) 890
MseI TTAA 3 cut(s) 455, 621, 1199
MslI CAYNNNNRTG 2 cut(s) 236, 530
Msp20I TGGCCA 1 cut(s) 890
MspR9I CCNGG 2 cut(s) 68, 411
MunI CAATTG 1 cut(s) 691
MvaI CCWGG 2 cut(s) 68, 411
MwoI GCNNNNNNNGC 3 cut(s) 179, 719, 896
NdeII GATC 5 cut(s) 442, 657, 1042, 1115, 1251
NlaIII CATG 8 cut(s) 314, 529, 559, 654, 658, 925, 1059, 1250
NlaIV GGNNCC 2 cut(s) 156, 871
NmuCI GTSAC 2 cut(s) 229, 829
NsiI ATGCAT 1 cut(s) 656
NspI RCATGY 2 cut(s) 529, 1059
OliI CACNNNNGTG 1 cut(s) 236
PagI TCATGA 2 cut(s) 310, 1246
PciI ACATGT 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 852
PflFI GACNNNGTC 1 cut(s) 496
PflMI CCANNNNNTGG 1 cut(s) 847
PkrI GCNGC 7 cut(s) 169, 172, 268, 898, 943, 1031, 1146
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PscI ACATGT 1 cut(s) 525
PshAI GACNNNNGTC 1 cut(s) 22
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 66, 409
PspGI CCWGG 2 cut(s) 66, 409
PspN4I GGNNCC 2 cut(s) 156, 871
PspPI GGNCC 2 cut(s) 181, 647
PstI CTGCAG 1 cut(s) 1149
PsuI RGATCY 2 cut(s) 442, 1251
PsyI GACNNNGTC 1 cut(s) 496
RsaI GTAC 3 cut(s) 465, 814, 871
RsaNI GTAC 3 cut(s) 464, 813, 870
RseI CAYNNNNRTG 2 cut(s) 236, 530
SacI GAGCTC 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 182
SaqAI TTAA 3 cut(s) 455, 621, 1199
SatI GCNGC 7 cut(s) 168, 171, 267, 897, 942, 1030, 1145
Sau3AI GATC 5 cut(s) 442, 657, 1042, 1115, 1251
Sau96I GGNCC 2 cut(s) 181, 647
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 68, 411
SduI GDGCHC 1 cut(s) 194
SfaNI GCATC 2 cut(s) 14, 361
SfcI CTRYAG 1 cut(s) 1145
SmiMI CAYNNNNRTG 2 cut(s) 236, 530
Sse9I AATT 6 cut(s) 551, 691, 743, 945, 954, 1157
SsiI CCGC 5 cut(s) 11, 581, 594, 1032, 1229
SspMI CTAG 2 cut(s) 174, 578
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 2 cut(s) 66, 409
TaaI ACNGT 8 cut(s) 235, 760, 807, 864, 1208, 1221, 1243, 1295
TaiI ACGT 2 cut(s) 786, 849
TaqI TCGA 2 cut(s) 1122, 1287
TasI AATT 6 cut(s) 551, 691, 743, 945, 954, 1157
Tru1I TTAA 3 cut(s) 455, 621, 1199
Tru9I TTAA 3 cut(s) 455, 621, 1199
TscAI CASTG 4 cut(s) 250, 679, 763, 1300
TseFI GTSAC 2 cut(s) 229, 829
TseI GCWGC 7 cut(s) 167, 170, 266, 896, 941, 1029, 1144
Tsp45I GTSAC 2 cut(s) 229, 829
TspRI CASTG 4 cut(s) 250, 679, 763, 1300
Tth111I GACNNNGTC 1 cut(s) 496
Van91I CCANNNNNTGG 1 cut(s) 847
XapI RAATTY 1 cut(s) 743
XceI RCATGY 2 cut(s) 529, 1059
XspI CTAG 2 cut(s) 174, 578
Zsp2I ATGCAT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.