MD17G1020500.v1.1

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014375
Physical Location & Seq
Forward (+)
1555767 .. 1558716
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1020500.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGGCTCCAGAACTCCAACAGGAGGAAGATTACACTACTGGCCCTGAAGCTGCTCATAAAACCAGAGTCACCGTCGTCGGCAGTGGCAACTGGGGTAGTGTTGCCGCCAAACTCATTGCCTCCAACACCCTCAAGCTCTCTTCTTTCCACGATGAAGTGAGAATGTGGGTGTTTGAGGAAACATTACCAAGTGGTGAGAAGCTCACAGATGCCATCAACCGCAGCAATGAAAATGTGAAATACCTGCCCGGCATTAAGCTGGGGAAAAATGTTGTTGCAGATCCAGACCTTGAGAATGCAGTGAACGGTGCGAACATGTTGGTGTTTGTTACTCCACATCAATTTATGGAGGGTATATGCAGGAGGCTTGTTGGGAAGGTAAAAGCGGATGTGGAGGCAATTTCCCTCATCAAAGAAATGGAGGTCAAGATAGAAGGCCCGTGCATGATCTCGACACTCATCTCACAGCAGTTGGGTATTAATTGTTGTGTTCTGATGGGAGCAAACATAGCTAATGAGATTGCTGTGGAGAAATTTAGTGAAGCCACAGTTGGATACAGAGAGAACAAAGAGATTGCACAGAAATGGGTTCAGCTCTTTAATACTTCCTACTTCATGGTCACACCTATCCAAGATGTGGAAGGGGTAGAACTATGTGGAACCCTGAAAAATGTTGTGGCCATAGCAGCAGGTTTTGTGGATGGGTTGGAGATGGGAAATAACACAAAGGCTGCAATTATGAGAATCGGTCTAAAAGAGATGCAGGCATTTTCCAAGATGTTATTTTCATCTGTCAAGGACACCACCTTCCTTGAAAGCTGTGGTGTCGCTGATCTCATCACAACATGCATGGGAGGAAGAAACAGGAAAGTTGCGGAAGCTTTTGCAAGGAGTGGAGGGAAAAGATCGTTTGATGAGCTTGAAGCAGAAATGCTGCAGGGTCAAAAATTACAGGGTGTCTCAACAGCAAAAGAGGTTTACGAGGTTTTAAGCCACCGCGGGTGGCTAGATTTCTTCCCACTTTTTGCAACAGTTCATGAGATCTGCATTGGCACTCTTCCTCCATCAGCCATAGTTGAACATAGTGAGCGCACGCCTAAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

369

Amino Acids

40.31

Weight (kDa)

5.37

Isoelectric Point (pI)

27.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 23 - 190 1.4e-38 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C PF07479 212 - 360 9.9e-46 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014375)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40610
fragaria_vesca FvH4_6g52110 FvH4_6g52110
malus_domestica MD09G1020100.v1.1 MD17G1020500.v1.1 MD17G1020700.v1.1
prunus_persica Prupe.3G296200_v2.0.a1 Prupe.3G296200_v2.0.a1
pyrus_communis pycom111g01680 pycom17g01730
rosa_chinensis RchiOBHm_Chr2g0173621
rosa_laevigata RLG00000022206
rosa_multiflora Rmu_sc0002538.1_g000016
rosa_roxburghii Rroxscaffold_2G00078740
rosa_rugosa Rorug02G0571700
rosa_samantha Rh2DG676300
rosa_wichuraiana Rw2G053400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 252, 680
AccB7I CCANNNNNTGG 1 cut(s) 637
AccII CGCG 1 cut(s) 999
AciI CCGC 6 cut(s) 105, 220, 386, 875, 997, 999
AclWI GGATC 1 cut(s) 275
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 1 cut(s) 66
AfiI CCNNNNNNNGG 2 cut(s) 22, 637
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 3 cut(s) 815, 923, 1079
AjuI GAANNNNNNNTTGG 2 cut(s) 534, 566
AluBI AGCT 9 cut(s) 50, 136, 202, 259, 512, 595, 819, 881, 919
AluI AGCT 9 cut(s) 50, 136, 202, 259, 512, 595, 819, 881, 919
Alw26I GTCTC 1 cut(s) 964
AlwI GGATC 1 cut(s) 275
AoxI GGCC 3 cut(s) 40, 436, 678
ApeKI GCWGC 5 cut(s) 50, 222, 686, 731, 934
ApoI RAATTY 1 cut(s) 533
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 1 cut(s) 1092
AspS9I GGNCC 2 cut(s) 41, 437
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 2 cut(s) 61, 206
BalI TGGCCA 1 cut(s) 680
BbvI GCAGC 5 cut(s) 37, 234, 698, 718, 921
BccI CCATC 5 cut(s) 221, 490, 695, 706, 1072
BciVI GTATCC 1 cut(s) 548
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 964
BfaI CTAG 2 cut(s) 1007, 1105
BfmI CTRYAG 1 cut(s) 935
BfuAI ACCTGC 2 cut(s) 252, 680
BfuI GTATCC 1 cut(s) 548
BglII AGATCT 1 cut(s) 1041
BisI GCNGC 6 cut(s) 51, 105, 223, 687, 732, 935
BlsI GCNGC 6 cut(s) 52, 106, 224, 688, 733, 936
Bme1390I CCNGG 1 cut(s) 249
BmgT120I GGNCC 2 cut(s) 41, 437
BmiI GGNNCC 2 cut(s) 6, 661
BmrFI CCNGG 1 cut(s) 249
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 2 cut(s) 199, 750
BmuI ACTGGG 1 cut(s) 100
BpuEI CTTGAG 2 cut(s) 116, 311
BpuMI CCSGG 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 997
BsaXI ACNNNNNCTCC 2 cut(s) 1045, 1075
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 637
Bse1I ACTGG 2 cut(s) 43, 95
Bse3DI GCAATG 2 cut(s) 114, 232
BseDI CCNNGG 1 cut(s) 997
BseGI GGATG 2 cut(s) 394, 706
BseLI CCNNNNNNNGG 2 cut(s) 22, 637
BseMI GCAATG 2 cut(s) 114, 232
BseNI ACTGG 2 cut(s) 43, 95
BseXI GCAGC 5 cut(s) 37, 234, 698, 718, 921
BseYI CCCAGC 1 cut(s) 259
Bsh1236I CGCG 1 cut(s) 999
BshFI GGCC 3 cut(s) 42, 438, 680
BsiSI CCGG 1 cut(s) 249
BslI CCNNNNNNNGG 2 cut(s) 22, 637
BsmAI GTCTC 1 cut(s) 964
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 3 cut(s) 42, 438, 680
Bsp143I GATC 5 cut(s) 280, 447, 832, 905, 1041
BspACI CCGC 6 cut(s) 105, 220, 386, 875, 997, 999
BspANI GGCC 3 cut(s) 42, 438, 680
BspFNI CGCG 1 cut(s) 999
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 2 cut(s) 6, 661
BspMAI CTGCAG 1 cut(s) 939
BspMI ACCTGC 2 cut(s) 252, 680
BspPI GGATC 1 cut(s) 275
BsrDI GCAATG 2 cut(s) 114, 232
BsrI ACTGG 2 cut(s) 43, 95
BssECI CCNNGG 1 cut(s) 997
BssMI GATC 5 cut(s) 280, 447, 832, 905, 1041
Bst4CI ACNGT 4 cut(s) 73, 308, 550, 1033
Bst6I CTCTTC 2 cut(s) 145, 1062
BstC8I GCNNGC 2 cut(s) 765, 1094
BstDSI CCRYGG 1 cut(s) 997
BstF5I GGATG 2 cut(s) 394, 706
BstFNI CGCG 1 cut(s) 999
BstHHI GCGC 1 cut(s) 1092
BstKTI GATC 5 cut(s) 283, 450, 835, 908, 1044
BstMAI GTCTC 1 cut(s) 964
BstMBI GATC 5 cut(s) 280, 447, 832, 905, 1041
BstMWI GCNNNNNNNGC 2 cut(s) 509, 686
BstNSI RCATGY 2 cut(s) 319, 849
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 935
BstUI CGCG 1 cut(s) 999
BstV1I GCAGC 5 cut(s) 37, 234, 698, 718, 921
BstX2I RGATCY 2 cut(s) 280, 1041
BstYI RGATCY 2 cut(s) 280, 1041
BsuI GTATCC 1 cut(s) 548
BsuRI GGCC 3 cut(s) 42, 438, 680
BtgI CCRYGG 1 cut(s) 997
BtsCI GGATG 2 cut(s) 394, 706
BtsI GCAGTG 2 cut(s) 88, 306
BtsIMutI CAGTG 2 cut(s) 88, 306
BveI ACCTGC 2 cut(s) 252, 680
Cac8I GCNNGC 2 cut(s) 765, 1094
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 1 cut(s) 1092
Cfr13I GGNCC 2 cut(s) 41, 437
Cfr42I CCGCGG 1 cut(s) 1000
CspCI CAANNNNNGTGG 2 cut(s) 793, 828
CviAII CATG 6 cut(s) 316, 445, 616, 846, 850, 1037
DpnI GATC 5 cut(s) 282, 449, 834, 907, 1043
DpnII GATC 5 cut(s) 280, 447, 832, 905, 1041
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 2 cut(s) 145, 1062
EarI CTCTTC 2 cut(s) 145, 1062
Eco57I CTGAAG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 851
FaeI CATG 6 cut(s) 319, 448, 619, 849, 853, 1040
FatI CATG 6 cut(s) 315, 444, 615, 845, 849, 1036
FauI CCCGC 1 cut(s) 992
Fnu4HI GCNGC 6 cut(s) 51, 105, 223, 687, 732, 935
FokI GGATG 2 cut(s) 401, 713
Fsp4HI GCNGC 6 cut(s) 51, 105, 223, 687, 732, 935
FspBI CTAG 2 cut(s) 1007, 1105
GlaI GCGC 1 cut(s) 1091
GluI GCNGC 6 cut(s) 51, 105, 223, 687, 732, 935
GsaI CCCAGC 1 cut(s) 263
HaeIII GGCC 3 cut(s) 42, 438, 680
HapII CCGG 1 cut(s) 249
HhaI GCGC 1 cut(s) 1092
Hin1II CATG 6 cut(s) 319, 448, 619, 849, 853, 1040
Hin6I GCGC 1 cut(s) 1090
HinP1I GCGC 1 cut(s) 1090
HindIII AAGCTT 1 cut(s) 879
HinfI GANTC 2 cut(s) 66, 744
HpaII CCGG 1 cut(s) 249
HphI GGTGA 2 cut(s) 61, 206
Hpy166II GTNNAC 2 cut(s) 304, 979
Hpy188I TCNGA 1 cut(s) 495
Hpy188III TCNNGA 5 cut(s) 8, 284, 427, 451, 1037
Hpy8I GTNNAC 2 cut(s) 304, 979
Hpy99I CGWCG 2 cut(s) 77, 80
HpyAV CCTTC 4 cut(s) 370, 428, 635, 817
HpyCH4III ACNGT 4 cut(s) 73, 308, 550, 1033
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 686
Hsp92II CATG 6 cut(s) 319, 448, 619, 849, 853, 1040
HspAI GCGC 1 cut(s) 1090
KspI CCGCGG 1 cut(s) 1000
Kzo9I GATC 5 cut(s) 280, 447, 832, 905, 1041
LmnI GCTCC 2 cut(s) 10, 500
Lsp1109I GCAGC 5 cut(s) 37, 234, 698, 718, 921
LweI GCATC 2 cut(s) 199, 750
MaeI CTAG 2 cut(s) 1007, 1105
MaeIII GTNAC 3 cut(s) 67, 328, 619
MalI GATC 5 cut(s) 282, 449, 834, 907, 1043
MboI GATC 5 cut(s) 280, 447, 832, 905, 1041
MboII GAAGA 5 cut(s) 38, 132, 870, 1006, 1049
MflI RGATCY 2 cut(s) 280, 1041
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 6 cut(s) 341, 399, 481, 533, 735, 947
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 4 cut(s) 40, 147, 532, 687
Mox20I TGGCCA 1 cut(s) 680
Mph1103I ATGCAT 1 cut(s) 851
MscI TGGCCA 1 cut(s) 680
MseI TTAA 4 cut(s) 255, 480, 600, 989
MslI CAYNNNNRTG 2 cut(s) 320, 583
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 999
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 301
MvnI CGCG 1 cut(s) 999
MwoI GCNNNNNNNGC 2 cut(s) 509, 686
NciI CCSGG 1 cut(s) 249
NdeII GATC 5 cut(s) 280, 447, 832, 905, 1041
NlaIII CATG 6 cut(s) 319, 448, 619, 849, 853, 1040
NlaIV GGNNCC 2 cut(s) 6, 661
NmuCI GTSAC 2 cut(s) 67, 619
NsiI ATGCAT 1 cut(s) 851
NspI RCATGY 2 cut(s) 319, 849
PagI TCATGA 1 cut(s) 1036
PciI ACATGT 1 cut(s) 315
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 744
PflMI CCANNNNNTGG 1 cut(s) 637
PkrI GCNGC 6 cut(s) 52, 106, 224, 688, 733, 936
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PscI ACATGT 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 6, 661
PspPI GGNCC 2 cut(s) 41, 437
PstI CTGCAG 1 cut(s) 939
PsuI RGATCY 2 cut(s) 280, 1041
RseI CAYNNNNRTG 2 cut(s) 320, 583
SacII CCGCGG 1 cut(s) 1000
SaqAI TTAA 4 cut(s) 255, 480, 600, 989
SatI GCNGC 6 cut(s) 51, 105, 223, 687, 732, 935
Sau3AI GATC 5 cut(s) 280, 447, 832, 905, 1041
Sau96I GGNCC 2 cut(s) 41, 437
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 1 cut(s) 249
SfaNI GCATC 2 cut(s) 199, 750
SfcI CTRYAG 1 cut(s) 935
Sfr303I CCGCGG 1 cut(s) 1000
SgrBI CCGCGG 1 cut(s) 1000
SmiMI CAYNNNNRTG 2 cut(s) 320, 583
SmlI CTYRAG 2 cut(s) 131, 290
SmoI CTYRAG 2 cut(s) 131, 290
Sse9I AATT 6 cut(s) 341, 399, 481, 533, 735, 947
SsiI CCGC 6 cut(s) 105, 220, 386, 875, 997, 999
SspMI CTAG 2 cut(s) 1007, 1105
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 4 cut(s) 73, 308, 550, 1033
TaqI TCGA 1 cut(s) 452
TaqII GACCGA 1 cut(s) 737
TasI AATT 6 cut(s) 341, 399, 481, 533, 735, 947
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 1 cut(s) 744
Tru1I TTAA 4 cut(s) 255, 480, 600, 989
Tru9I TTAA 4 cut(s) 255, 480, 600, 989
TscAI CASTG 2 cut(s) 88, 306
TseFI GTSAC 2 cut(s) 67, 619
TseI GCWGC 5 cut(s) 50, 222, 686, 731, 934
Tsp45I GTSAC 2 cut(s) 67, 619
TspDTI ATGAA 5 cut(s) 168, 243, 604, 777, 1025
TspRI CASTG 2 cut(s) 88, 306
Van91I CCANNNNNTGG 1 cut(s) 637
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 533
XceI RCATGY 2 cut(s) 319, 849
XspI CTAG 2 cut(s) 1007, 1105
Zsp2I ATGCAT 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.