MD09G1021000.v1.1

Pre-rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008064
Physical Location & Seq
Reverse (-)
1272261 .. 1273875
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1021000.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGAAATCGAGAGAGAGAACAAAAGGAAGAAATCGATGAAGGAAGGTGGGAAGGATGAGAAGCAGCGAGGGGTTTGCTATTTGGGTCGGATTCCGCCGCGCATGGATCCGTCGACGCTTCGTCAGATGCTCTCCCAGTACGGAGAGATACAAAGGCTTTATCTTGCACCTCAAGATTCTTCTGGTCATGTTCAAATCAAAGCTGGTAAATTTCAGAGGCAAAATTTTTCAGAAGGGTGGGTTGAATTTTCTGACAAAAGGGTTGCTGAGAGAGTTGCTAATATGTTAAATGGTGAACAGATAGGTGGGAAGAAGAGGTCATCTTTCTACTATGATCTTTGGAATATCAAGTATTTGAGTAAATTCAAGTGGGATAATCTTACCGAAGAGATTGCTTACAAGAAAGCCACTCGGGAGCAAAAACTAGCTTTAGAAATTTCTGCTGCTAAGAAGGAGCGTGATTTCTATCTCACTAAAGTTGAGAAGTCCCGTGCTCTGAGTGCCATAGATGAAAGATTGAAGAAGAGGCAAAGGCAAAGGGTCGAAGAATATTCAGGGAGCAATCCGGATCTCTCTGATAGCCAACCGGAACATCAGGTAATTCGCCGGTTCCGACAGAAGAAACCTGTTGCAAATAATGCTGCAAAAACCAAACCTCGTCTTTCTGGAGATGTACTGTCAGCGGTATTTGGTGGTTCTTAG

Protein Analysis

234

Amino Acids

27.04

Weight (kDa)

9.9

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 27 - 105 8.8e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 113
AccII CGCG 1 cut(s) 100
AccIII TCCGGA 1 cut(s) 565
AciI CCGC 3 cut(s) 95, 98, 683
AclWI GGATC 3 cut(s) 101, 114, 576
AcsI RAATTY 5 cut(s) 209, 223, 245, 362, 435
AfaI GTAC 2 cut(s) 140, 675
AgsI TTSAA 4 cut(s) 194, 245, 367, 520
AhdI GACNNNNNGTC 1 cut(s) 120
AluBI AGCT 2 cut(s) 203, 428
AluI AGCT 2 cut(s) 203, 428
Alw21I GWGCWC 1 cut(s) 496
AlwI GGATC 3 cut(s) 101, 114, 576
Ama87I CYCGRG 1 cut(s) 411
Aor13HI TCCGGA 1 cut(s) 565
ApeKI GCWGC 3 cut(s) 64, 443, 641
ApoI RAATTY 5 cut(s) 209, 223, 245, 362, 435
AspLEI GCGC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 411
BamHI GGATCC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 3 cut(s) 76, 430, 628
BcgI CGANNNNNNTGC 4 cut(s) 57, 91, 374, 408
BfaI CTAG 1 cut(s) 425
BisI GCNGC 4 cut(s) 65, 98, 444, 642
BlsI GCNGC 4 cut(s) 66, 99, 445, 643
BmeRI GACNNNNNGTC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 411
BmiI GGNNCC 2 cut(s) 108, 611
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 1 cut(s) 117
BmuI ACTGGG 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 465
BsaWI WCCGGW 2 cut(s) 565, 586
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 565
BseCI ATCGAT 1 cut(s) 35
BseGI GGATG 1 cut(s) 61
BseJI GATNNNNATC 1 cut(s) 465
BseMII CTCAG 2 cut(s) 258, 488
BseNI ACTGG 1 cut(s) 136
BseXI GCAGC 3 cut(s) 76, 430, 628
Bsh1236I CGCG 1 cut(s) 100
BshVI ATCGAT 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 496
BsiHKCI CYCGRG 1 cut(s) 411
BsiSI CCGG 3 cut(s) 566, 587, 607
BslFI GGGAC 1 cut(s) 472
BsmFI GGGAC 1 cut(s) 472
BsoBI CYCGRG 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 496
Bsp13I TCCGGA 1 cut(s) 565
Bsp143I GATC 3 cut(s) 106, 334, 568
BspACI CCGC 3 cut(s) 95, 98, 683
BspCNI CTCAG 2 cut(s) 259, 489
BspDI ATCGAT 1 cut(s) 35
BspEI TCCGGA 1 cut(s) 565
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 108, 611
BspPI GGATC 3 cut(s) 101, 114, 576
BsrFI RCCGGY 1 cut(s) 606
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 606
BssMI GATC 3 cut(s) 106, 334, 568
Bst4CI ACNGT 1 cut(s) 678
Bst6I CTCTTC 3 cut(s) 308, 381, 518
BstAPI GCANNNNNTGC 1 cut(s) 638
BstDEI CTNAG 4 cut(s) 267, 447, 497, 699
BstF5I GGATG 1 cut(s) 61
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 3 cut(s) 109, 337, 571
BstMBI GATC 3 cut(s) 106, 334, 568
BstMWI GCNNNNNNNGC 2 cut(s) 500, 638
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 3 cut(s) 76, 430, 628
BstX2I RGATCY 2 cut(s) 106, 568
BstYI RGATCY 2 cut(s) 106, 568
Bsu15I ATCGAT 1 cut(s) 35
BsuTUI ATCGAT 1 cut(s) 35
BtsCI GGATG 1 cut(s) 61
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 606
ClaI ATCGAT 1 cut(s) 35
CseI GACGC 1 cut(s) 124
Csp6I GTAC 2 cut(s) 139, 674
CviAII CATG 2 cut(s) 103, 188
CviJI RGCY 5 cut(s) 157, 203, 407, 428, 582
CviKI_1 RGCY 5 cut(s) 157, 203, 407, 428, 582
CviQI GTAC 2 cut(s) 139, 674
DdeI CTNAG 4 cut(s) 267, 447, 497, 699
DpnI GATC 3 cut(s) 108, 336, 570
DpnII GATC 3 cut(s) 106, 334, 568
DriI GACNNNNNGTC 1 cut(s) 120
Eam1104I CTCTTC 3 cut(s) 308, 381, 518
Eam1105I GACNNNNNGTC 1 cut(s) 120
EarI CTCTTC 3 cut(s) 308, 381, 518
EciI GGCGGA 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 411
FaeI CATG 2 cut(s) 106, 191
FaiI YATR 5 cut(s) 104, 189, 284, 333, 506
FaqI GGGAC 1 cut(s) 472
FatI CATG 2 cut(s) 102, 187
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 4 cut(s) 65, 98, 444, 642
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 4 cut(s) 65, 98, 444, 642
FspBI CTAG 1 cut(s) 425
GlaI GCGC 1 cut(s) 101
GluI GCNGC 4 cut(s) 65, 98, 444, 642
GsuI CTGGAG 1 cut(s) 687
HapII CCGG 3 cut(s) 566, 587, 607
HgaI GACGC 1 cut(s) 124
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 106, 191
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 2 cut(s) 91, 176
HpaII CCGG 3 cut(s) 566, 587, 607
HphI GGTGA 1 cut(s) 305
Hpy166II GTNNAC 2 cut(s) 114, 296
Hpy188I TCNGA 8 cut(s) 90, 126, 216, 232, 253, 498, 577, 614
Hpy188III TCNNGA 5 cut(s) 10, 173, 413, 566, 666
Hpy8I GTNNAC 2 cut(s) 114, 296
Hpy99I CGWCG 2 cut(s) 115, 118
HpyAV CCTTC 5 cut(s) 34, 38, 46, 227, 445
HpyCH4III ACNGT 1 cut(s) 678
HpyCH4V TGCA 3 cut(s) 167, 632, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 500, 638
HpyF3I CTNAG 4 cut(s) 267, 447, 497, 699
Hsp92II CATG 2 cut(s) 106, 191
HspAI GCGC 1 cut(s) 100
Kpn2I TCCGGA 1 cut(s) 565
Kzo9I GATC 3 cut(s) 106, 334, 568
LmnI GCTCC 3 cut(s) 415, 454, 558
Lsp1109I GCAGC 3 cut(s) 76, 430, 628
LweI GCATC 1 cut(s) 117
MaeI CTAG 1 cut(s) 425
MalI GATC 3 cut(s) 108, 336, 570
MboI GATC 3 cut(s) 106, 334, 568
MboII GAAGA 9 cut(s) 40, 171, 322, 325, 398, 532, 535, 557, 631
MflI RGATCY 2 cut(s) 106, 568
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 6 cut(s) 209, 223, 245, 362, 435, 600
MmeI TCCRAC 2 cut(s) 68, 637
MnlI CCTC 6 cut(s) 62, 180, 210, 309, 519, 666
MroI TCCGGA 1 cut(s) 565
MseI TTAA 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 3 cut(s) 566, 587, 607
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 2 cut(s) 500, 638
NdeII GATC 3 cut(s) 106, 334, 568
NlaIII CATG 2 cut(s) 106, 191
NlaIV GGNNCC 2 cut(s) 108, 611
PcsI WCGNNNNNNNCGW 1 cut(s) 610
PfeI GAWTC 2 cut(s) 91, 176
PkrI GCNGC 4 cut(s) 66, 99, 445, 643
PspN4I GGNNCC 2 cut(s) 108, 611
PsuI RGATCY 2 cut(s) 106, 568
RsaI GTAC 2 cut(s) 140, 675
RsaNI GTAC 2 cut(s) 139, 674
SalI GTCGAC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 4 cut(s) 65, 98, 444, 642
Sau3AI GATC 3 cut(s) 106, 334, 568
SduI GDGCHC 1 cut(s) 496
SetI ASST 9 cut(s) 49, 172, 205, 307, 320, 430, 600, 628, 658
SfaNI GCATC 1 cut(s) 117
SgrDI CGTCGACG 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
Sse9I AATT 6 cut(s) 209, 223, 245, 362, 435, 600
SsiI CCGC 3 cut(s) 95, 98, 683
SspI AATATT 1 cut(s) 551
SspMI CTAG 1 cut(s) 425
TaaI ACNGT 1 cut(s) 678
TaqI TCGA 4 cut(s) 9, 35, 113, 543
TasI AATT 6 cut(s) 209, 223, 245, 362, 435, 600
TatI WGTACW 1 cut(s) 673
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 2 cut(s) 91, 176
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseI GCWGC 3 cut(s) 64, 443, 641
TspDTI ATGAA 2 cut(s) 53, 525
TspGWI ACGGA 2 cut(s) 99, 156
XapI RAATTY 5 cut(s) 209, 223, 245, 362, 435
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.