MD09G1042000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007717
Physical Location & Seq
Forward (+)
2730408 .. 2732196
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1042000.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGCGGGAAGCGACGCTCGGAAGCAGCTTCTGAACTTAATTCACGATTTCGCATCCGAAAAGTCGCACGGAGAGCGACGAGTGGTAAGTCTGAGGAAGCGAATCGAAGAGCTCGGATCCGAGCTGGAAATTGCAAATGCGGAGCTCGAAGAAGCCAAGCGCACCAAGGAAACCGCCGAGCAAGAGGTCAAAGGCTTCGAAGTCGAATTGGCGATGAACGAAGCAACGATTCAAACGCTGGAGTTGAGGATTTCTCATACGCAAGATGAGATTTCCGCCGTTGGATCCGAAGTAGAAGCTCTTAAGAACAAAGAAGCTGCTTCGAGGGACGAGTTTATAAGCGAAATGTTTGAGATCAACGCTAAGATAAGAAAGTTCCAAGAGTCAATTGCTGGCCATATTCATGAGGAAGAGTACTGTGGAAGTGCAGAAGAAGAAGATCCCAAGCTAGGAAAGGAGGAGGTTACTGAAGGTGATTTAAGAGAACTTGAGGATATGCTTGCTGGTGTGGTGTCTCAGACAACCAAAGCGGAAGAAGAATACAAAGCTGAACAGAATACTCAAAAGCAGGTCCAGCAGGTGCTGAGTGATTGTGAAAGGAAGGTGTTCTTGATGGAGGAGTTGTTCAAGGCAACGAAAGAAGTGCATGATTTGACAAGATATCCTTTTCAAGAATGCATTGGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04260 ko04261 ko05410 ko05414 map04260 map04261 map05410 map05414
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

26.13

Weight (kDa)

4.71

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AarI CACCTGC 1 cut(s) 568
Acc36I ACCTGC 2 cut(s) 559, 568
AciI CCGC 5 cut(s) 5, 140, 174, 276, 530
AclWI GGATC 5 cut(s) 111, 124, 279, 292, 434
AcoI YGGCCR 1 cut(s) 394
AcuI CTGAAG 1 cut(s) 489
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 449
AflII CTTAAG 1 cut(s) 302
AgsI TTSAA 3 cut(s) 233, 628, 671
AloI GAACNNNNNNTCC 2 cut(s) 608, 640
AluBI AGCT 8 cut(s) 28, 112, 124, 145, 299, 317, 448, 548
AluI AGCT 8 cut(s) 28, 112, 124, 145, 299, 317, 448, 548
Alw21I GWGCWC 2 cut(s) 114, 147
Alw26I GTCTC 1 cut(s) 519
AlwI GGATC 5 cut(s) 111, 124, 279, 292, 434
AlwNI CAGNNNCTG 2 cut(s) 31, 583
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 2 cut(s) 25, 317
Asp700I GAANNNNTTC 1 cut(s) 605
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 571
AsuHPI GGTGA 1 cut(s) 485
AsuII TTCGAA 1 cut(s) 198
AvaII GGWCC 1 cut(s) 571
BalI TGGCCA 1 cut(s) 396
BamHI GGATCC 2 cut(s) 116, 284
BanII GRGCYC 2 cut(s) 114, 147
Bbv12I GWGCWC 2 cut(s) 114, 147
BbvI GCAGC 2 cut(s) 37, 304
BccI CCATC 1 cut(s) 607
BceAI ACGGC 1 cut(s) 263
BcgI CGANNNNNNTGC 2 cut(s) 625, 659
BcoDI GTCTC 1 cut(s) 519
BfaI CTAG 1 cut(s) 449
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 2 cut(s) 559, 568
BisI GCNGC 2 cut(s) 26, 318
BlsI GCNGC 2 cut(s) 27, 319
BmcAI AGTACT 1 cut(s) 416
Bme18I GGWCC 1 cut(s) 571
BmgT120I GGNCC 1 cut(s) 571
BmiI GGNNCC 2 cut(s) 118, 286
BmsI GCATC 1 cut(s) 62
BplI GAGNNNNNCTC 2 cut(s) 238, 270
BpmI CTGGAG 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 608, 638
Bsc4I CCNNNNNNNGG 1 cut(s) 449
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMII CTCAG 3 cut(s) 83, 530, 575
BseRI GAGGAG 2 cut(s) 473, 632
BseXI GCAGC 2 cut(s) 37, 304
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 1 cut(s) 396
BsiHKAI GWGCWC 2 cut(s) 114, 147
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 519
BsmFI GGGAC 1 cut(s) 341
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 1 cut(s) 396
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 2 cut(s) 114, 147
Bsp143I GATC 4 cut(s) 116, 284, 354, 439
BspACI CCGC 5 cut(s) 5, 140, 174, 276, 530
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 3 cut(s) 84, 529, 576
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 118, 286
BspMI ACCTGC 2 cut(s) 559, 568
BspPI GGATC 5 cut(s) 111, 124, 279, 292, 434
BspQI GCTCTTC 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 198
BspTI CTTAAG 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 4 cut(s) 116, 284, 354, 439
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 2 cut(s) 102, 405
BstAFI CTTAAG 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 394, 501
BstDEI CTNAG 4 cut(s) 92, 363, 516, 584
BstF5I GGATG 1 cut(s) 53
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 4 cut(s) 119, 287, 357, 442
BstMAI GTCTC 1 cut(s) 519
BstMBI GATC 4 cut(s) 116, 284, 354, 439
BstMWI GCNNNNNNNGC 2 cut(s) 73, 574
BstV1I GCAGC 2 cut(s) 37, 304
BstX2I RGATCY 3 cut(s) 116, 284, 439
BstYI RGATCY 3 cut(s) 116, 284, 439
BsuRI GGCC 1 cut(s) 396
BtgZI GCGATG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 53
BveI ACCTGC 2 cut(s) 559, 568
Cac8I GCNNGC 2 cut(s) 394, 501
CaiI CAGNNNCTG 2 cut(s) 31, 583
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 571
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 415
CviAII CATG 2 cut(s) 404, 647
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 4 cut(s) 92, 363, 516, 584
DpnI GATC 4 cut(s) 118, 286, 356, 441
DpnII GATC 4 cut(s) 116, 284, 354, 439
EaeI YGGCCR 1 cut(s) 394
Eam1104I CTCTTC 2 cut(s) 102, 405
EarI CTCTTC 2 cut(s) 102, 405
EciI GGCGGA 1 cut(s) 265
Ecl136II GAGCTC 2 cut(s) 112, 145
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 114, 147
Eco32I GATATC 1 cut(s) 662
Eco47I GGWCC 1 cut(s) 571
Eco53kI GAGCTC 2 cut(s) 112, 145
Eco57I CTGAAG 1 cut(s) 489
EcoICRI GAGCTC 2 cut(s) 112, 145
EcoRV GATATC 1 cut(s) 662
EcoT14I CCWWGG 1 cut(s) 165
EcoT22I ATGCAT 1 cut(s) 680
EcoT38I GRGCYC 2 cut(s) 114, 147
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 407, 650
FaiI YATR 6 cut(s) 258, 338, 399, 405, 497, 648
FalI AAGNNNNNCTT 4 cut(s) 593, 625, 649, 681
FaqI GGGAC 1 cut(s) 341
FatI CATG 2 cut(s) 403, 646
Fnu4HI GCNGC 2 cut(s) 26, 318
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 2 cut(s) 114, 147
Fsp4HI GCNGC 2 cut(s) 26, 318
FspBI CTAG 1 cut(s) 449
GlaI GCGC 1 cut(s) 161
GluI GCNGC 2 cut(s) 26, 318
GsuI CTGGAG 1 cut(s) 260
HaeIII GGCC 1 cut(s) 396
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 162
Hin1II CATG 2 cut(s) 407, 650
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 3 cut(s) 102, 229, 383
HphI GGTGA 1 cut(s) 485
Hpy188I TCNGA 8 cut(s) 21, 33, 58, 93, 116, 121, 289, 519
Hpy188III TCNNGA 4 cut(s) 44, 404, 610, 671
Hpy99I CGWCG 2 cut(s) 17, 81
HpyAV CCTTC 2 cut(s) 464, 595
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 4 cut(s) 134, 428, 646, 678
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 574
HpyF3I CTNAG 4 cut(s) 92, 363, 516, 584
Hsp92II CATG 2 cut(s) 407, 650
HspAI GCGC 1 cut(s) 160
Kzo9I GATC 4 cut(s) 116, 284, 354, 439
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 7 cut(s) 110, 224, 378, 489, 554, 563, 587
Lsp1109I GCAGC 2 cut(s) 37, 304
LweI GCATC 1 cut(s) 62
MaeI CTAG 1 cut(s) 449
MaeIII GTNAC 2 cut(s) 463, 683
MalI GATC 4 cut(s) 118, 286, 356, 441
MboI GATC 4 cut(s) 116, 284, 354, 439
MboII GAAGA 8 cut(s) 119, 161, 422, 443, 446, 449, 545, 548
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 3 cut(s) 116, 284, 439
MhlI GDGCHC 2 cut(s) 114, 147
MlsI TGGCCA 1 cut(s) 396
MluCI AATT 4 cut(s) 39, 129, 206, 387
MluNI TGGCCA 1 cut(s) 396
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 9 cut(s) 87, 178, 240, 318, 400, 451, 454, 484, 610
Mox20I TGGCCA 1 cut(s) 396
Mph1103I ATGCAT 1 cut(s) 680
MroXI GAANNNNTTC 1 cut(s) 605
MscI TGGCCA 1 cut(s) 396
MseI TTAA 3 cut(s) 38, 303, 479
MslI CAYNNNNRTG 1 cut(s) 402
Msp20I TGGCCA 1 cut(s) 396
MspCI CTTAAG 1 cut(s) 302
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 680
MwoI GCNNNNNNNGC 2 cut(s) 73, 574
NdeII GATC 4 cut(s) 116, 284, 354, 439
NlaIII CATG 2 cut(s) 407, 650
NlaIV GGNNCC 2 cut(s) 118, 286
NmeAIII GCCGAG 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 680
NspV TTCGAA 1 cut(s) 198
PagI TCATGA 1 cut(s) 403
PaqCI CACCTGC 1 cut(s) 568
PciSI GCTCTTC 1 cut(s) 102
PctI GAATGC 1 cut(s) 680
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 102, 229
PkrI GCNGC 2 cut(s) 27, 319
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
PsiI TTATAA 1 cut(s) 338
Psp124BI GAGCTC 2 cut(s) 114, 147
PspN4I GGNNCC 2 cut(s) 118, 286
PspPI GGNCC 1 cut(s) 571
PstNI CAGNNNCTG 2 cut(s) 31, 583
PsuI RGATCY 3 cut(s) 116, 284, 439
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 2 cut(s) 114, 147
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 3 cut(s) 38, 303, 479
SatI GCNGC 2 cut(s) 26, 318
Sau3AI GATC 4 cut(s) 116, 284, 354, 439
Sau96I GGNCC 1 cut(s) 571
ScaI AGTACT 1 cut(s) 416
SchI GAGTC 1 cut(s) 392
SduI GDGCHC 2 cut(s) 114, 147
SfaNI GCATC 1 cut(s) 62
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 2 cut(s) 302, 488
SmoI CTYRAG 2 cut(s) 302, 488
Sse9I AATT 4 cut(s) 39, 129, 206, 387
SsiI CCGC 5 cut(s) 5, 140, 174, 276, 530
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 2 cut(s) 114, 147
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 419
TaqI TCGA 5 cut(s) 105, 147, 198, 204, 323
TasI AATT 4 cut(s) 39, 129, 206, 387
TatI WGTACW 1 cut(s) 414
TfiI GAWTC 2 cut(s) 102, 229
Tru1I TTAA 3 cut(s) 38, 303, 479
Tru9I TTAA 3 cut(s) 38, 303, 479
TseI GCWGC 2 cut(s) 25, 317
TspDTI ATGAA 2 cut(s) 230, 392
TspGWI ACGGA 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 571
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 449
ZrmI AGTACT 1 cut(s) 416
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.