pycom111g03290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0007717
Physical Location & Seq
Forward (+)
1997062 .. 1998863
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03290.4

Sequence Viewer

Length: 633 bp
ATGGCGGGAAGCGACGCTCGGAAGCAGCTTCTGAACCTAATTCACGATTTCGCATCCGAAAAGTCGCACGGAGAGCGAAGAGTGGTAAGTTTGAGGAAGCGAATCGAAGAGCTCGGATCCGAGCTGGAAATTGCAAATGCGGAGCTCGAAGAAGCCAAGCGCACCAAGGAAACCGCCGAGCAAGAGGTCAAAGGCTATGAAGTCGAATTGGCGATGAACGAAGCAACGGTTCAAACGCTGAAGCTAAAATCTTTGATTACTGATTCTAATTTTATGCTGTTTTTGTTTGGATCATGCAAAAGTACGAAGAAGCTGCGTCGAGAAGAAGATCCCAAGCTAGGAGAGGAGGAGGTTACTGAAGGTGACTTAAGAGAACTTGAGGATATGCTTGGTGGTGTGGTGTCTCAGACAACCAAAGCGGAAGAAGAATACAAAGCTGAACAGAATACTCAAAAGCAGGTGAAGAAATCGGTCTTGGAAACATTACGAATGGGTCTTTACGGTTCGCTACAAGTCCAGCAGGCGCTGGGTGATTGTGAAAGGAAGGTGTTCTTGATGGAGGAGACGTTCAAGGCAACGAAAGAAGTGCATGATTTGACAAGATATCCTTTTCAAGAATGCATTGGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04260 ko04261 ko05410 ko05414 map04260 map04261 map05410 map05414
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.97

Weight (kDa)

5.13

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 448
Acc36I ACCTGC 1 cut(s) 448
AciI CCGC 4 cut(s) 5, 140, 174, 419
AclWI GGATC 4 cut(s) 111, 124, 298, 323
AcuI CTGAAG 2 cut(s) 260, 378
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 1 cut(s) 338
AflII CTTAAG 1 cut(s) 367
AgsI TTSAA 3 cut(s) 233, 571, 614
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AloI GAACNNNNNNTCC 2 cut(s) 551, 583
AluBI AGCT 8 cut(s) 28, 112, 124, 145, 244, 313, 337, 437
AluI AGCT 8 cut(s) 28, 112, 124, 145, 244, 313, 337, 437
Alw21I GWGCWC 2 cut(s) 114, 147
Alw26I GTCTC 2 cut(s) 408, 557
AlwI GGATC 4 cut(s) 111, 124, 298, 323
AlwNI CAGNNNCTG 2 cut(s) 31, 526
ApeKI GCWGC 2 cut(s) 25, 313
Asp700I GAANNNNTTC 1 cut(s) 548
AspLEI GCGC 2 cut(s) 162, 526
AsuHPI GGTGA 3 cut(s) 374, 472, 542
BamHI GGATCC 1 cut(s) 116
BanII GRGCYC 2 cut(s) 114, 147
Bbv12I GWGCWC 2 cut(s) 114, 147
BbvI GCAGC 2 cut(s) 37, 300
BccI CCATC 1 cut(s) 550
BcgI CGANNNNNNTGC 4 cut(s) 295, 329, 568, 602
BcoDI GTCTC 2 cut(s) 408, 557
BfaI CTAG 1 cut(s) 338
BfoI RGCGCY 1 cut(s) 527
BfrI CTTAAG 1 cut(s) 367
BfuAI ACCTGC 1 cut(s) 448
BisI GCNGC 2 cut(s) 26, 314
BlsI GCNGC 2 cut(s) 27, 315
BmiI GGNNCC 1 cut(s) 118
BmsI GCATC 1 cut(s) 62
BpuEI CTTGAG 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 419
BseRI GAGGAG 3 cut(s) 359, 362, 575
BseXI GCAGC 2 cut(s) 37, 300
BseYI CCCAGC 1 cut(s) 526
BsiHKAI GWGCWC 2 cut(s) 114, 147
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 2 cut(s) 408, 557
BsmBI CGTCTC 1 cut(s) 557
BsmI GAATGC 1 cut(s) 623
Bsp1286I GDGCHC 2 cut(s) 114, 147
Bsp143I GATC 3 cut(s) 116, 290, 328
BspACI CCGC 4 cut(s) 5, 140, 174, 419
BspCNI CTCAG 1 cut(s) 418
BspLI GGNNCC 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 4 cut(s) 111, 124, 298, 323
BspQI GCTCTTC 1 cut(s) 102
BspTI CTTAAG 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 116, 290, 328
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 229, 503
Bst6I CTCTTC 2 cut(s) 73, 102
BstAFI CTTAAG 1 cut(s) 367
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 2 cut(s) 162, 526
BstKTI GATC 3 cut(s) 119, 293, 331
BstMAI GTCTC 2 cut(s) 408, 557
BstMBI GATC 3 cut(s) 116, 290, 328
BstMWI GCNNNNNNNGC 1 cut(s) 73
BstV1I GCAGC 2 cut(s) 37, 300
BstX2I RGATCY 2 cut(s) 116, 328
BstYI RGATCY 2 cut(s) 116, 328
BtgZI GCGATG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 53
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 522
CaiI CAGNNNCTG 2 cut(s) 31, 526
CfoI GCGC 2 cut(s) 162, 526
CseI GACGC 2 cut(s) 23, 305
Csp6I GTAC 1 cut(s) 303
CviAII CATG 2 cut(s) 294, 590
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 1 cut(s) 405
DpnI GATC 3 cut(s) 118, 292, 330
DpnII GATC 3 cut(s) 116, 290, 328
Eam1104I CTCTTC 2 cut(s) 73, 102
EarI CTCTTC 2 cut(s) 73, 102
Ecl136II GAGCTC 2 cut(s) 112, 145
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 114, 147
Eco32I GATATC 1 cut(s) 605
Eco53kI GAGCTC 2 cut(s) 112, 145
Eco57I CTGAAG 2 cut(s) 260, 378
EcoICRI GAGCTC 2 cut(s) 112, 145
EcoRV GATATC 1 cut(s) 605
EcoT14I CCWWGG 1 cut(s) 165
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 2 cut(s) 114, 147
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 557
FaeI CATG 2 cut(s) 297, 593
FaiI YATR 5 cut(s) 198, 275, 295, 386, 591
FalI AAGNNNNNCTT 4 cut(s) 536, 568, 592, 624
FatI CATG 2 cut(s) 293, 589
Fnu4HI GCNGC 2 cut(s) 26, 314
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 2 cut(s) 114, 147
Fsp4HI GCNGC 2 cut(s) 26, 314
FspBI CTAG 1 cut(s) 338
GlaI GCGC 2 cut(s) 161, 525
GluI GCNGC 2 cut(s) 26, 314
GsaI CCCAGC 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 527
HgaI GACGC 2 cut(s) 23, 305
HhaI GCGC 2 cut(s) 162, 526
Hin1II CATG 2 cut(s) 297, 593
Hin6I GCGC 2 cut(s) 160, 524
HinP1I GCGC 2 cut(s) 160, 524
HinfI GANTC 2 cut(s) 102, 263
HphI GGTGA 3 cut(s) 374, 472, 542
Hpy188I TCNGA 6 cut(s) 21, 33, 58, 116, 121, 408
Hpy188III TCNNGA 4 cut(s) 44, 320, 553, 614
Hpy99I CGWCG 2 cut(s) 17, 321
HpyAV CCTTC 2 cut(s) 353, 538
HpyCH4III ACNGT 2 cut(s) 229, 503
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 4 cut(s) 134, 297, 589, 621
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 1 cut(s) 566
Hsp92II CATG 2 cut(s) 297, 593
HspAI GCGC 2 cut(s) 160, 524
Kzo9I GATC 3 cut(s) 116, 290, 328
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 5 cut(s) 110, 443, 506, 512, 530
Lsp1109I GCAGC 2 cut(s) 37, 300
LweI GCATC 1 cut(s) 62
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 3 cut(s) 352, 362, 626
MalI GATC 3 cut(s) 118, 292, 330
MboI GATC 3 cut(s) 116, 290, 328
MboII GAAGA 9 cut(s) 90, 119, 161, 319, 335, 338, 434, 437, 475
MflI RGATCY 2 cut(s) 116, 328
MhlI GDGCHC 2 cut(s) 114, 147
MluCI AATT 4 cut(s) 39, 129, 206, 268
MnlI CCTC 7 cut(s) 87, 178, 337, 340, 343, 373, 553
Mph1103I ATGCAT 1 cut(s) 623
MroXI GAANNNNTTC 1 cut(s) 548
MseI TTAA 1 cut(s) 368
MspCI CTTAAG 1 cut(s) 367
Mva1269I GAATGC 1 cut(s) 623
MwoI GCNNNNNNNGC 1 cut(s) 73
NdeII GATC 3 cut(s) 116, 290, 328
NlaIII CATG 2 cut(s) 297, 593
NlaIV GGNNCC 1 cut(s) 118
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 362
NsiI ATGCAT 1 cut(s) 623
PaqCI CACCTGC 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 102
PctI GAATGC 1 cut(s) 623
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 2 cut(s) 102, 263
PkrI GCNGC 2 cut(s) 27, 315
Psp124BI GAGCTC 2 cut(s) 114, 147
PspFI CCCAGC 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 118
PstNI CAGNNNCTG 2 cut(s) 31, 526
PsuI RGATCY 2 cut(s) 116, 328
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
SacI GAGCTC 2 cut(s) 114, 147
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 368
SatI GCNGC 2 cut(s) 26, 314
Sau3AI GATC 3 cut(s) 116, 290, 328
SduI GDGCHC 2 cut(s) 114, 147
SfaNI GCATC 1 cut(s) 62
SmlI CTYRAG 2 cut(s) 367, 377
SmoI CTYRAG 2 cut(s) 367, 377
Sse9I AATT 4 cut(s) 39, 129, 206, 268
SsiI CCGC 4 cut(s) 5, 140, 174, 419
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 2 cut(s) 114, 147
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 229, 503
TaiI ACGT 1 cut(s) 569
TaqI TCGA 4 cut(s) 105, 147, 204, 319
TaqII GACCGA 1 cut(s) 460
TasI AATT 4 cut(s) 39, 129, 206, 268
TfiI GAWTC 2 cut(s) 102, 263
Tru1I TTAA 1 cut(s) 368
Tru9I TTAA 1 cut(s) 368
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 2 cut(s) 25, 313
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 2 cut(s) 213, 230
TspGWI ACGGA 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.