MD09G1047900.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015677
Physical Location & Seq
Reverse (-)
3136412 .. 3138936
2525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1047900.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGCAACGGAGGGGTAAGTCGCGGTTGCTGCCGCCGCCGGCGACGAGCATCAAGGACCTGAATGCGGATACGCTGGGGCAATGCACCAGCTCCATTAGCCTCCAAGACCTCTCCAACTTTGCCGTGACCTGCAAATATCTCAGAAAAATGGCATATTCTGACGCTATTTGGGAGCGGTTGTTCAGGTACTTCTTCTACTCCTTTTTGTTCTTGTCAAGAAAAAATTTGAATAAACATCACATAGACTACCAGGGGTGTGCGCAAATACCACATGAACTGAAGATATTTCGTTTGGCACGACGAGTATTGGTGCGATTTCTAACGGAGATTGACACATTTACACTAGAAGTAGAGGAGCGCTGGCCCCAGAAAACACTACCTTCTGCCATCTCTATAACTTCGGGATTTAGAGAAGCCTATTTATCTCGGCGTATGGATGGGCTGCAGTTCAAGTTTTCCGATCCTTTCACTTTGCAGATGAACACCAACTCAAATCCTTTCGACCAAGTATTATTGACCGAGAAAGATTTAATCTTCTCTCAGGGTTCACTGATACGCATGATAAGGCCTGAGGATTGGTTAAGTAGAGGACCCGTCACTCTTAGTGATCACAGTGAAAGAATCACTTGCATGAGGTTGTTTCCACTGAATGAAACATCTTTGTATCTACATAAACTATATAAACCGGAGAAGGTTTTAGTAACTTCAAGCGATGATAGTTCCATTCGGCTATGGAGGAAGGGTTATTGCTGGCGACAATTTAGGGGGCATAGTAGCCAAGTTACAACCCTCTCAGATAAATTGTTAGGCAATGGAGCCGGAAAAGTATTTGCAAGCGGAGGGGAGGATGGCACGGTTCGCCTTTGGTCTCTTAGCTTTTTCAGGAAACAGGGCAAGCATTCTTTGGGGACTTTCTTCGGGCATAAAAATCCCATCCAGTTGATGTCAGTTTCCATGGACAACCCCTCTCACTTGGTGATGGTGTGGGATACGGAGGCAGCACTGCTTGCCAGTTCTTCGTTTGCCGGTGGGGCGACTTCTGTTCTCGGTTACCCTATAGGCATCAAGTGTCATGAACATCTAGCCTATGTTGCTGCTGGTTCCTCTGTGGTAGCAATCGATTTGAGGACGATGCAGAAAGTTGTCATTGCAGCAGCAGGTGCAATTCTGTATTCTTTTCAAGCAATGCCTTCCAAATCTTTATTCTGCGTAGGCAGCAATGGCAGAGCGATGCTTTACGATACAAGGAAGAACTTGGGCACCTTGAATTCAGAACCAATAGTGGTAGATGGTGGACACGGAGGGCCAGTGACGTACTTGCACATGGATCCCTACAAAATAGTTACAGGAAGTCCGGTGGATACGCATTTTAATGTTTTGGACGTCAACACTGGTGCACTGATCAATTCCTTGACATGTATTGAATATGGAACAAATAACAGCACAAGGTGCTGTGCCCTGGCTGTCAACGGGAGTCGAATTGTTATTGGTAGTGTCAACGATGTTGAAGAAGTCGGATGTCTGCTCTTTAGAGACTTCGCCAATGCAAATCGGATGTCTCCTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

523

Amino Acids

58.3

Weight (kDa)

9.12

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 231 - 321 1.3e-09 WDR5 beta-propeller domain
WD40 PF00400 249 - 290 3.5e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 271 - 499 2.4e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49170
malus_domestica MD09G1047900.v1.1 MD17G1048000.v1.1 MD17G1048100.v1.1
prunus_persica Prupe.3G271100_v2.0.a1
pyrus_communis pycom17g04280
rosa_chinensis RchiOBHm_Chr2g0169041
rosa_laevigata RLG00000021826
rosa_multiflora Rmu_ssc0000393.1_g000025
rosa_roxburghii Rroxscaffold_2G00082510
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG608000 Rh2BG620700 Rh2CG589600 Rh2DG631300
rosa_wichuraiana Rw2G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1148
AatII GACGTC 1 cut(s) 1386
Acc16I TGCGCA 1 cut(s) 261
Acc36I ACCTGC 2 cut(s) 137, 1148
AccB1I GGYRCC 1 cut(s) 1259
AccBSI CCGCTC 1 cut(s) 175
AccII CGCG 1 cut(s) 22
AciI CCGC 6 cut(s) 22, 32, 35, 65, 175, 837
AclWI GGATC 3 cut(s) 455, 1322, 1335
AcsI RAATTY 2 cut(s) 223, 1267
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 1383
AdeI CACNNNGTG 2 cut(s) 976, 1449
AfaI GTAC 2 cut(s) 188, 1316
AfeI AGCGCT 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 64
AflIII ACRYGT 1 cut(s) 1415
AgsI TTSAA 7 cut(s) 229, 451, 708, 1181, 1267, 1424, 1508
AjnI CCWGG 2 cut(s) 249, 1458
AjuI GAANNNNNNNTTGG 2 cut(s) 1187, 1219
AloI GAACNNNNNNTCC 2 cut(s) 164, 196
AluBI AGCT 2 cut(s) 90, 876
AluI AGCT 2 cut(s) 90, 876
Alw21I GWGCWC 1 cut(s) 1399
Alw26I GTCTC 3 cut(s) 873, 1527, 1563
Alw44I GTGCAC 1 cut(s) 1395
AlwI GGATC 3 cut(s) 455, 1322, 1335
Aor51HI AGCGCT 1 cut(s) 359
AoxI GGCC 3 cut(s) 362, 566, 1304
ApaLI GTGCAC 1 cut(s) 1395
ApeKI GCWGC 7 cut(s) 28, 442, 998, 1094, 1151, 1154, 1215
ApoI RAATTY 2 cut(s) 223, 1267
ArsI GACNNNNNNTTYG 1 cut(s) 1541
AspLEI GCGC 2 cut(s) 262, 360
AspS9I GGNCC 4 cut(s) 55, 363, 590, 1304
AsuHPI GGTGA 1 cut(s) 988
AvaII GGWCC 2 cut(s) 55, 590
AxyI CCTNAGG 1 cut(s) 570
BaeGI GKGCMC 3 cut(s) 1262, 1399, 1459
BamHI GGATCC 1 cut(s) 1327
BanI GGYRCC 1 cut(s) 1259
Bbv12I GWGCWC 1 cut(s) 1399
BbvI GCAGC 7 cut(s) 15, 429, 1010, 1081, 1163, 1166, 1227
BccI CCATC 6 cut(s) 395, 431, 842, 941, 973, 1283
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 2 cut(s) 251, 1460
BciVI GTATCC 3 cut(s) 61, 982, 1354
BclI TGATCA 2 cut(s) 607, 1401
BcoDI GTCTC 3 cut(s) 873, 1527, 1563
BfaI CTAG 2 cut(s) 344, 1082
BfmI CTRYAG 2 cut(s) 443, 1056
BfoI RGCGCY 1 cut(s) 361
BfuAI ACCTGC 2 cut(s) 137, 1148
BfuI GTATCC 3 cut(s) 61, 982, 1354
BglI GCCNNNNNGGC 1 cut(s) 1031
BisI GCNGC 9 cut(s) 29, 32, 35, 443, 999, 1095, 1152, 1155, 1216
BlsI GCNGC 9 cut(s) 30, 33, 36, 444, 1000, 1096, 1153, 1156, 1217
Bme1390I CCNGG 2 cut(s) 251, 1460
Bme18I GGWCC 2 cut(s) 55, 590
BmgT120I GGNCC 4 cut(s) 55, 363, 590, 1304
BmiI GGNNCC 6 cut(s) 365, 592, 817, 1102, 1261, 1329
BmrFI CCNGG 2 cut(s) 251, 1460
BmsI GCATC 4 cut(s) 57, 1071, 1122, 1221
Bsa29I ATCGAT 1 cut(s) 1119
BsaHI GRCGYC 1 cut(s) 1383
BsaI GGTCTC 1 cut(s) 873
BsaJI CCNNGG 3 cut(s) 250, 954, 1458
BsaWI WCCGGW 2 cut(s) 685, 1354
BsaXI ACNNNNNCTCC 6 cut(s) 164, 194, 727, 757, 1293, 1323
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse118I RCCGGY 2 cut(s) 37, 1025
Bse1I ACTGG 4 cut(s) 937, 1011, 1307, 1396
Bse21I CCTNAGG 1 cut(s) 570
Bse3DI GCAATG 5 cut(s) 86, 817, 1146, 1191, 1225
BseBI CCWGG 2 cut(s) 251, 1460
BseCI ATCGAT 1 cut(s) 1119
BseDI CCNNGG 3 cut(s) 250, 954, 1458
BseGI GGATG 5 cut(s) 442, 853, 933, 1523, 1560
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 5 cut(s) 86, 817, 1146, 1191, 1225
BseMII CTCAG 4 cut(s) 154, 554, 561, 807
BseNI ACTGG 4 cut(s) 937, 1011, 1307, 1396
BseRI GAGGAG 2 cut(s) 368, 1551
BseSI GKGCMC 3 cut(s) 1262, 1399, 1459
BseXI GCAGC 7 cut(s) 15, 429, 1010, 1081, 1163, 1166, 1227
BseYI CCCAGC 1 cut(s) 73
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 3 cut(s) 364, 568, 1306
BshNI GGYRCC 1 cut(s) 1259
BshVI ATCGAT 1 cut(s) 1119
BsiHKAI GWGCWC 1 cut(s) 1399
BsiSI CCGG 5 cut(s) 38, 686, 819, 1026, 1355
BslFI GGGAC 1 cut(s) 922
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 3 cut(s) 873, 1527, 1563
BsmFI GGGAC 1 cut(s) 922
BsmI GAATGC 2 cut(s) 67, 898
BsnI GGCC 3 cut(s) 364, 568, 1306
Bso31I GGTCTC 1 cut(s) 873
Bsp1286I GDGCHC 3 cut(s) 1262, 1399, 1459
Bsp143I GATC 4 cut(s) 460, 607, 1327, 1401
Bsp19I CCATGG 1 cut(s) 954
BspACI CCGC 6 cut(s) 22, 32, 35, 65, 175, 837
BspANI GGCC 3 cut(s) 364, 568, 1306
BspCNI CTCAG 4 cut(s) 153, 553, 562, 806
BspDI ATCGAT 1 cut(s) 1119
BspFNI CGCG 1 cut(s) 22
BspHI TCATGA 1 cut(s) 1072
BspLI GGNNCC 6 cut(s) 365, 592, 817, 1102, 1261, 1329
BspMAI CTGCAG 1 cut(s) 447
BspMI ACCTGC 2 cut(s) 137, 1148
BspPI GGATC 3 cut(s) 455, 1322, 1335
BspT107I GGYRCC 1 cut(s) 1259
BspTNI GGTCTC 1 cut(s) 873
BsrBI CCGCTC 1 cut(s) 175
BsrDI GCAATG 5 cut(s) 86, 817, 1146, 1191, 1225
BsrFI RCCGGY 2 cut(s) 37, 1025
BsrI ACTGG 4 cut(s) 937, 1011, 1307, 1396
BssAI RCCGGY 2 cut(s) 37, 1025
BssECI CCNNGG 3 cut(s) 250, 954, 1458
BssMI GATC 4 cut(s) 460, 607, 1327, 1401
BssNI GRCGYC 1 cut(s) 1383
BssT1I CCWWGG 1 cut(s) 954
Bst2UI CCWGG 2 cut(s) 251, 1460
Bst4CI ACNGT 2 cut(s) 614, 856
BstACI GRCGYC 1 cut(s) 1383
BstAPI GCANNNNNTGC 3 cut(s) 1007, 1160, 1449
BstC8I GCNNGC 6 cut(s) 39, 362, 752, 835, 896, 1008
BstDEI CTNAG 6 cut(s) 140, 540, 570, 602, 793, 872
BstDSI CCRYGG 1 cut(s) 954
BstEII GGTNACC 1 cut(s) 1049
BstF5I GGATG 5 cut(s) 442, 853, 933, 1523, 1560
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 361
BstHHI GCGC 2 cut(s) 262, 360
BstKTI GATC 4 cut(s) 463, 610, 1330, 1404
BstMAI GTCTC 3 cut(s) 873, 1527, 1563
BstMBI GATC 4 cut(s) 460, 607, 1327, 1401
BstNI CCWGG 2 cut(s) 251, 1460
BstNSI RCATGY 1 cut(s) 1419
BstPI GGTNACC 1 cut(s) 1049
BstSCI CCNGG 2 cut(s) 249, 1458
BstSFI CTRYAG 2 cut(s) 443, 1056
BstSLI GKGCMC 3 cut(s) 1262, 1399, 1459
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 7 cut(s) 15, 429, 1010, 1081, 1163, 1166, 1227
BstX2I RGATCY 1 cut(s) 1327
BstYI RGATCY 1 cut(s) 1327
Bsu15I ATCGAT 1 cut(s) 1119
Bsu36I CCTNAGG 1 cut(s) 570
BsuI GTATCC 3 cut(s) 61, 982, 1354
BsuRI GGCC 3 cut(s) 364, 568, 1306
BsuTUI ATCGAT 1 cut(s) 1119
BtgI CCRYGG 1 cut(s) 954
BtgZI GCGATG 2 cut(s) 726, 1244
BtsCI GGATG 5 cut(s) 442, 853, 933, 1523, 1560
BtsI GCAGTG 1 cut(s) 1001
BtsIMutI CAGTG 7 cut(s) 548, 619, 644, 1001, 1314, 1389, 1397
BveI ACCTGC 2 cut(s) 137, 1148
Cac8I GCNNGC 6 cut(s) 39, 362, 752, 835, 896, 1008
CciI TCATGA 1 cut(s) 1072
CfoI GCGC 2 cut(s) 262, 360
Cfr10I RCCGGY 2 cut(s) 37, 1025
Cfr13I GGNCC 4 cut(s) 55, 363, 590, 1304
ClaI ATCGAT 1 cut(s) 1119
CseI GACGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 187, 1315
CviAII CATG 7 cut(s) 272, 559, 631, 955, 1073, 1324, 1416
CviQI GTAC 2 cut(s) 187, 1315
DdeI CTNAG 6 cut(s) 140, 540, 570, 602, 793, 872
DpnI GATC 4 cut(s) 462, 609, 1329, 1403
DpnII GATC 4 cut(s) 460, 607, 1327, 1401
DraIII CACNNNGTG 2 cut(s) 976, 1449
Eco130I CCWWGG 1 cut(s) 954
Eco147I AGGCCT 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 873
Eco47I GGWCC 2 cut(s) 55, 590
Eco47III AGCGCT 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 570
Eco91I GGTNACC 1 cut(s) 1049
EcoO109I RGGNCCY 2 cut(s) 55, 590
EcoO65I GGTNACC 1 cut(s) 1049
EcoRI GAATTC 1 cut(s) 1267
EcoRII CCWGG 2 cut(s) 249, 1458
EcoT14I CCWWGG 1 cut(s) 954
ErhI CCWWGG 1 cut(s) 954
FaeI CATG 7 cut(s) 275, 562, 634, 958, 1076, 1327, 1419
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FaqI GGGAC 1 cut(s) 922
FatI CATG 7 cut(s) 271, 558, 630, 954, 1072, 1323, 1415
FbaI TGATCA 2 cut(s) 607, 1401
Fnu4HI GCNGC 9 cut(s) 29, 32, 35, 443, 999, 1095, 1152, 1155, 1216
FokI GGATG 4 cut(s) 449, 860, 920, 1530
Fsp4HI GCNGC 9 cut(s) 29, 32, 35, 443, 999, 1095, 1152, 1155, 1216
FspBI CTAG 2 cut(s) 344, 1082
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 2 cut(s) 261, 359
GluI GCNGC 9 cut(s) 29, 32, 35, 443, 999, 1095, 1152, 1155, 1216
GsaI CCCAGC 1 cut(s) 77
HaeII RGCGCY 1 cut(s) 361
HaeIII GGCC 3 cut(s) 364, 568, 1306
HapII CCGG 5 cut(s) 38, 686, 819, 1026, 1355
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 262, 360
Hin1I GRCGYC 1 cut(s) 1383
Hin1II CATG 7 cut(s) 275, 562, 634, 958, 1076, 1327, 1419
Hin6I GCGC 2 cut(s) 260, 358
HinP1I GCGC 2 cut(s) 260, 358
HincII GTYRAC 3 cut(s) 1387, 1468, 1498
HindII GTYRAC 3 cut(s) 1387, 1468, 1498
HinfI GANTC 2 cut(s) 621, 1474
HpaII CCGG 5 cut(s) 38, 686, 819, 1026, 1355
HphI GGTGA 1 cut(s) 988
Hpy166II GTNNAC 6 cut(s) 548, 1295, 1387, 1397, 1468, 1498
Hpy188I TCNGA 7 cut(s) 143, 160, 460, 796, 1273, 1517, 1554
Hpy188III TCNNGA 4 cut(s) 216, 402, 883, 1073
Hpy8I GTNNAC 6 cut(s) 548, 1295, 1387, 1397, 1468, 1498
Hpy99I CGWCG 2 cut(s) 46, 303
HpyAV CCTTC 4 cut(s) 390, 685, 733, 1200
HpyCH4III ACNGT 2 cut(s) 614, 856
HpyCH4IV ACGT 2 cut(s) 1313, 1383
HpyF3I CTNAG 6 cut(s) 140, 540, 570, 602, 793, 872
HpySE526I ACGT 2 cut(s) 1313, 1383
Hsp92I GRCGYC 1 cut(s) 1383
Hsp92II CATG 7 cut(s) 275, 562, 634, 958, 1076, 1327, 1419
HspAI GCGC 2 cut(s) 260, 358
KroI GCCGGC 1 cut(s) 37
KroNI GCCGGC 1 cut(s) 39
Ksp22I TGATCA 2 cut(s) 607, 1401
Kzo9I GATC 4 cut(s) 460, 607, 1327, 1401
LmnI GCTCC 4 cut(s) 95, 172, 355, 815
Lsp1109I GCAGC 7 cut(s) 15, 429, 1010, 1081, 1163, 1166, 1227
LweI GCATC 4 cut(s) 57, 1071, 1122, 1221
MaeI CTAG 2 cut(s) 344, 1082
MaeII ACGT 2 cut(s) 1313, 1383
MaeIII GTNAC 7 cut(s) 124, 595, 700, 781, 1049, 1309, 1342
MalI GATC 4 cut(s) 462, 609, 1329, 1403
MbiI CCGCTC 1 cut(s) 175
MboI GATC 4 cut(s) 460, 607, 1327, 1401
MboII GAAGA 7 cut(s) 184, 292, 526, 907, 1008, 1261, 1520
MflI RGATCY 1 cut(s) 1327
MhlI GDGCHC 3 cut(s) 1262, 1399, 1459
MluCI AATT 7 cut(s) 223, 758, 800, 1164, 1267, 1405, 1479
MlyI GAGTC 1 cut(s) 1483
MmeI TCCRAC 2 cut(s) 138, 1495
MreI CGCCGGCG 1 cut(s) 37
MroNI GCCGGC 1 cut(s) 37
MseI TTAA 3 cut(s) 530, 581, 1371
MslI CAYNNNNRTG 2 cut(s) 629, 1371
MspI CCGG 5 cut(s) 38, 686, 819, 1026, 1355
MspR9I CCNGG 2 cut(s) 251, 1460
Mva1269I GAATGC 2 cut(s) 67, 898
MvaI CCWGG 2 cut(s) 251, 1460
MvnI CGCG 1 cut(s) 22
NaeI GCCGGC 1 cut(s) 39
NcoI CCATGG 1 cut(s) 954
NdeII GATC 4 cut(s) 460, 607, 1327, 1401
NgoMIV GCCGGC 1 cut(s) 37
NlaIII CATG 7 cut(s) 275, 562, 634, 958, 1076, 1327, 1419
NlaIV GGNNCC 6 cut(s) 365, 592, 817, 1102, 1261, 1329
NmeAIII GCCGAG 1 cut(s) 406
NmuCI GTSAC 3 cut(s) 124, 595, 1309
NsbI TGCGCA 1 cut(s) 261
NspI RCATGY 1 cut(s) 1419
PagI TCATGA 1 cut(s) 1072
PaqCI CACCTGC 1 cut(s) 1148
PceI AGGCCT 1 cut(s) 568
PciI ACATGT 1 cut(s) 1415
PcsI WCGNNNNNNNCGW 1 cut(s) 295
PctI GAATGC 2 cut(s) 67, 898
PdiI GCCGGC 1 cut(s) 39
PfeI GAWTC 1 cut(s) 621
PkrI GCNGC 9 cut(s) 30, 33, 36, 444, 1000, 1096, 1153, 1156, 1217
PleI GAGTC 1 cut(s) 1482
PpsI GAGTC 1 cut(s) 1482
PpuMI RGGWCCY 2 cut(s) 55, 590
PscI ACATGT 1 cut(s) 1415
Psp5II RGGWCCY 2 cut(s) 55, 590
Psp6I CCWGG 2 cut(s) 249, 1458
PspEI GGTNACC 1 cut(s) 1049
PspFI CCCAGC 1 cut(s) 73
PspGI CCWGG 2 cut(s) 249, 1458
PspN4I GGNNCC 6 cut(s) 365, 592, 817, 1102, 1261, 1329
PspPI GGNCC 4 cut(s) 55, 363, 590, 1304
PspPPI RGGWCCY 2 cut(s) 55, 590
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 1327
RsaI GTAC 2 cut(s) 188, 1316
RsaNI GTAC 2 cut(s) 187, 1315
RseI CAYNNNNRTG 2 cut(s) 629, 1371
SaqAI TTAA 3 cut(s) 530, 581, 1371
SatI GCNGC 9 cut(s) 29, 32, 35, 443, 999, 1095, 1152, 1155, 1216
Sau3AI GATC 4 cut(s) 460, 607, 1327, 1401
Sau96I GGNCC 4 cut(s) 55, 363, 590, 1304
SchI GAGTC 1 cut(s) 1483
ScrFI CCNGG 2 cut(s) 251, 1460
SduI GDGCHC 3 cut(s) 1262, 1399, 1459
SfaNI GCATC 4 cut(s) 57, 1071, 1122, 1221
SfcI CTRYAG 2 cut(s) 443, 1056
SgrAI CRCCGGYG 1 cut(s) 37
SinI GGWCC 2 cut(s) 55, 590
SmiMI CAYNNNNRTG 2 cut(s) 629, 1371
Sse9I AATT 7 cut(s) 223, 758, 800, 1164, 1267, 1405, 1479
SseBI AGGCCT 1 cut(s) 568
SsiI CCGC 6 cut(s) 22, 32, 35, 65, 175, 837
SspMI CTAG 2 cut(s) 344, 1082
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 2 cut(s) 249, 1458
StyI CCWWGG 1 cut(s) 954
TaaI ACNGT 2 cut(s) 614, 856
TaiI ACGT 2 cut(s) 1316, 1386
TaqI TCGA 3 cut(s) 501, 1119, 1477
TaqII GACCGA 1 cut(s) 533
TasI AATT 7 cut(s) 223, 758, 800, 1164, 1267, 1405, 1479
TauI GCSGC 2 cut(s) 34, 37
TfiI GAWTC 1 cut(s) 621
Tru1I TTAA 3 cut(s) 530, 581, 1371
Tru9I TTAA 3 cut(s) 530, 581, 1371
TscAI CASTG 7 cut(s) 555, 619, 651, 1008, 1314, 1396, 1404
TseFI GTSAC 3 cut(s) 124, 595, 1309
TseI GCWGC 7 cut(s) 28, 442, 998, 1094, 1151, 1154, 1215
Tsp45I GTSAC 3 cut(s) 124, 595, 1309
TspDTI ATGAA 4 cut(s) 288, 494, 666, 1089
TspGWI ACGGA 4 cut(s) 22, 338, 1007, 1314
TspRI CASTG 7 cut(s) 555, 619, 651, 1008, 1314, 1396, 1404
VneI GTGCAC 1 cut(s) 1395
VpaK11BI GGWCC 2 cut(s) 55, 590
XapI RAATTY 2 cut(s) 223, 1267
XceI RCATGY 1 cut(s) 1419
XspI CTAG 2 cut(s) 344, 1082
ZraI GACGTC 1 cut(s) 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.