MD17G1048100.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015677
Physical Location & Seq
Reverse (-)
3496812 .. 3499679
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1048100.v1.1.491

Sequence Viewer

Length: 963 bp
ATGTCGAAACGAAACACAGAAAGAGGGAGTGTTTTGGTAACTTCAAGCCTTGACCATTCCATTCGGCTATGGTGGAAGGGTGCTTGCCGTAGATGTTTTAGAGGTTCCATTGGCCCCATTTCAGCATTGTCAGATAAGTTGTTAGGCGATGGAACAGGAAAAGTATTGGCAAGTGGAGGGAAAGATGGCAATGTTAGCCTTTGGTCTCTTAGCTCCTGTGGTAGGCGTGGCCAACATGCTTTAAAGGCTACTTCCTATGGGCGTGAGAAGCCCATTCGATTGATGTCTGTTGCTGGGCACAACACCTCTCACTTGGTAACCGTTTCAAGAGATTCTAAGGTGAGTGTGTGGGATACAAATGCTCGTTCTTCATGCTGTGTGGGGATGACTTCTACTCTTGGTGCCCCTATAGACATGAAGTGTCATGAACATTTGGTCTATGTTGCTGCTGGTTCCTCCATTACGGCAATTGATTTGAGGAAAAAGGAAAAAGTTGCCATTGCTGCAGTGGATGCAAAGATATGTTCTTTTCAGGCAATGCCTTCCAAATCTTTATTCTGCATAGGCAGCCATGGCAGAGCGATGCTTTACGATATAAGGATGAACTGTGGCACCCAGAAATCAGAACCAATAGCGGAGTTAGAGGCTGGACACAAGGGGCCGGTGAATTACTTGCACATGGACCCATACAAAATAGTCACCGGAAGCCCGAAGGATCAGCATGTTAAAATTTGGGAGGCCGACACTGGTGCCCGAACCAATTCCTTGACCTGTGGTCCAGAAGGTGTAACAAATAGAAGATCTGAGCTTGGTGCCCTAGCTGTAAATGGGAGTCGAATAGTTACTGGAATCGTCAATGTCTGCGATGATGTGAGAGGATGTTTACGCTTTAGGGATTTCATAGATGCTTCTTGCACTGACATGAATCGCGATGAACCTCCTGCTACAAGGTTTTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

321

Amino Acids

34.64

Weight (kDa)

9.25

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 7 - 153 1.8e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 121 7.5e-06 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 52 - 191 3.8e-13 THOC3 beta-propeller domain
WD40_Prp19 PF24814 52 - 246 1.7e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 95 - 258 1.2e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 101 - 261 7.2e-07 WDHD1 first WD40 domain
Beta-prop_EIPR1 PF23609 142 - 245 4.3e-06 EIPR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49170
malus_domestica MD09G1047900.v1.1 MD17G1048000.v1.1 MD17G1048100.v1.1
prunus_persica Prupe.3G271100_v2.0.a1
pyrus_communis pycom17g04280
rosa_chinensis RchiOBHm_Chr2g0169041
rosa_laevigata RLG00000021826
rosa_multiflora Rmu_ssc0000393.1_g000025
rosa_roxburghii Rroxscaffold_2G00082510
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG608000 Rh2BG620700 Rh2CG589600 Rh2DG631300
rosa_wichuraiana Rw2G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 401, 611, 749, 812
AccII CGCG 1 cut(s) 930
AciI CCGC 1 cut(s) 635
AclWI GGATC 1 cut(s) 723
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 1 cut(s) 729
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 2 cut(s) 45, 327
AhdI GACNNNNNGTC 1 cut(s) 774
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AluBI AGCT 3 cut(s) 213, 808, 821
AluI AGCT 3 cut(s) 213, 808, 821
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 1 cut(s) 723
AoxI GGCC 4 cut(s) 112, 229, 659, 738
ApeKI GCWGC 3 cut(s) 446, 503, 567
ApoI RAATTY 1 cut(s) 729
Asp700I GAANNNNTTC 1 cut(s) 760
AspS9I GGNCC 4 cut(s) 113, 659, 682, 776
AsuHPI GGTGA 3 cut(s) 352, 676, 691
AvaII GGWCC 2 cut(s) 682, 776
BaeGI GKGCMC 4 cut(s) 300, 406, 754, 817
BalI TGGCCA 1 cut(s) 231
BanI GGYRCC 4 cut(s) 401, 611, 749, 812
BbvI GCAGC 3 cut(s) 433, 490, 579
BccI CCATC 2 cut(s) 143, 179
BceAI ACGGC 2 cut(s) 72, 480
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciVI GTATCC 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 1 cut(s) 818
BfmI CTRYAG 2 cut(s) 408, 504
BfuI GTATCC 1 cut(s) 346
BglII AGATCT 1 cut(s) 800
BisI GCNGC 3 cut(s) 447, 504, 568
BlsI GCNGC 3 cut(s) 448, 505, 569
Bme18I GGWCC 2 cut(s) 682, 776
BmeRI GACNNNNNGTC 1 cut(s) 774
BmgT120I GGNCC 4 cut(s) 113, 659, 682, 776
BmiI GGNNCC 9 cut(s) 106, 115, 403, 454, 613, 660, 684, 751, 814
BmsI GCATC 3 cut(s) 502, 573, 895
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 571
BsaWI WCCGGW 1 cut(s) 701
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 661
Bse1I ACTGG 2 cut(s) 751, 850
Bse3DI GCAATG 3 cut(s) 196, 498, 543
BseDI CCNNGG 1 cut(s) 571
BseGI GGATG 4 cut(s) 390, 517, 606, 884
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMI GCAATG 3 cut(s) 196, 498, 543
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 2 cut(s) 751, 850
BseSI GKGCMC 4 cut(s) 300, 406, 754, 817
BseXI GCAGC 3 cut(s) 433, 490, 579
BseYI CCCAGC 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 930
BshFI GGCC 4 cut(s) 114, 231, 661, 740
BshNI GGYRCC 4 cut(s) 401, 611, 749, 812
BsiSI CCGG 2 cut(s) 662, 702
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 4 cut(s) 114, 231, 661, 740
Bso31I GGTCTC 1 cut(s) 210
Bsp1286I GDGCHC 4 cut(s) 300, 406, 754, 817
Bsp143I GATC 2 cut(s) 715, 800
Bsp19I CCATGG 1 cut(s) 571
Bsp68I TCGCGA 1 cut(s) 930
BspACI CCGC 1 cut(s) 635
BspANI GGCC 4 cut(s) 114, 231, 661, 740
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 930
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 9 cut(s) 106, 115, 403, 454, 613, 660, 684, 751, 814
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 1 cut(s) 723
BspT107I GGYRCC 4 cut(s) 401, 611, 749, 812
BspTNI GGTCTC 1 cut(s) 210
BsrDI GCAATG 3 cut(s) 196, 498, 543
BsrFI RCCGGY 1 cut(s) 661
BsrI ACTGG 2 cut(s) 751, 850
BssAI RCCGGY 1 cut(s) 661
BssECI CCNNGG 1 cut(s) 571
BssMI GATC 2 cut(s) 715, 800
BssT1I CCWWGG 1 cut(s) 571
Bst4CI ACNGT 2 cut(s) 322, 608
BstAPI GCANNNNNTGC 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 3 cut(s) 209, 336, 804
BstDSI CCRYGG 1 cut(s) 571
BstEII GGTNACC 1 cut(s) 316
BstENI CCTNNNNNAGG 1 cut(s) 220
BstF5I GGATG 4 cut(s) 390, 517, 606, 884
BstFNI CGCG 1 cut(s) 930
BstKTI GATC 2 cut(s) 718, 803
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 2 cut(s) 715, 800
BstMWI GCNNNNNNNGC 7 cut(s) 195, 245, 268, 503, 512, 567, 573
BstNSI RCATGY 2 cut(s) 239, 725
BstPI GGTNACC 1 cut(s) 316
BstSFI CTRYAG 2 cut(s) 408, 504
BstSLI GKGCMC 4 cut(s) 300, 406, 754, 817
BstUI CGCG 1 cut(s) 930
BstV1I GCAGC 3 cut(s) 433, 490, 579
BstX2I RGATCY 1 cut(s) 800
BstYI RGATCY 1 cut(s) 800
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 4 cut(s) 114, 231, 661, 740
BtgI CCRYGG 1 cut(s) 571
BtgZI GCGATG 4 cut(s) 162, 596, 879, 945
BtsCI GGATG 4 cut(s) 390, 517, 606, 884
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 3 cut(s) 513, 744, 915
BtuMI TCGCGA 1 cut(s) 930
Cac8I GCNNGC 1 cut(s) 85
CciI TCATGA 1 cut(s) 424
Cfr10I RCCGGY 1 cut(s) 661
Cfr13I GGNCC 4 cut(s) 113, 659, 682, 776
CviAII CATG 8 cut(s) 236, 372, 415, 425, 572, 679, 722, 922
DdeI CTNAG 3 cut(s) 209, 336, 804
DpnI GATC 2 cut(s) 717, 802
DpnII GATC 2 cut(s) 715, 800
DraI TTTAAA 1 cut(s) 243
DriI GACNNNNNGTC 1 cut(s) 774
EaeI YGGCCR 1 cut(s) 229
Eam1105I GACNNNNNGTC 1 cut(s) 774
Eco130I CCWWGG 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 2 cut(s) 682, 776
Eco91I GGTNACC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 571
FaeI CATG 8 cut(s) 239, 375, 418, 428, 575, 682, 725, 925
FatI CATG 8 cut(s) 235, 371, 414, 424, 571, 678, 721, 921
Fnu4HI GCNGC 3 cut(s) 447, 504, 568
FokI GGATG 4 cut(s) 397, 524, 613, 891
Fsp4HI GCNGC 3 cut(s) 447, 504, 568
FspBI CTAG 1 cut(s) 818
GluI GCNGC 3 cut(s) 447, 504, 568
GsaI CCCAGC 1 cut(s) 297
HaeIII GGCC 4 cut(s) 114, 231, 661, 740
HapII CCGG 2 cut(s) 662, 702
Hin1II CATG 8 cut(s) 239, 375, 418, 428, 575, 682, 725, 925
HinfI GANTC 4 cut(s) 332, 832, 849, 925
HpaII CCGG 2 cut(s) 662, 702
HphI GGTGA 3 cut(s) 352, 676, 691
Hpy166II GTNNAC 1 cut(s) 884
Hpy188I TCNGA 3 cut(s) 133, 625, 805
Hpy188III TCNNGA 4 cut(s) 327, 425, 779, 929
Hpy8I GTNNAC 1 cut(s) 884
HpyAV CCTTC 4 cut(s) 70, 552, 706, 776
HpyCH4III ACNGT 2 cut(s) 322, 608
HpyCH4V TGCA 5 cut(s) 506, 515, 561, 676, 915
HpyF10VI GCNNNNNNNGC 7 cut(s) 195, 245, 268, 503, 512, 567, 573
HpyF3I CTNAG 3 cut(s) 209, 336, 804
Hsp92II CATG 8 cut(s) 239, 375, 418, 428, 575, 682, 725, 925
Kzo9I GATC 2 cut(s) 715, 800
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 3 cut(s) 433, 490, 579
LweI GCATC 3 cut(s) 502, 573, 895
MaeI CTAG 1 cut(s) 818
MaeIII GTNAC 5 cut(s) 37, 316, 697, 787, 841
MalI GATC 2 cut(s) 717, 802
MboI GATC 2 cut(s) 715, 800
MboII GAAGA 2 cut(s) 360, 810
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 1 cut(s) 800
MhlI GDGCHC 4 cut(s) 300, 406, 754, 817
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 4 cut(s) 468, 667, 729, 760
MluNI TGGCCA 1 cut(s) 231
MlyI GAGTC 1 cut(s) 841
Mox20I TGGCCA 1 cut(s) 231
MroXI GAANNNNTTC 1 cut(s) 760
MscI TGGCCA 1 cut(s) 231
MseI TTAA 2 cut(s) 242, 726
MslI CAYNNNNRTG 1 cut(s) 920
Msp20I TGGCCA 1 cut(s) 231
MspI CCGG 2 cut(s) 662, 702
MunI CAATTG 1 cut(s) 468
MvnI CGCG 1 cut(s) 930
MwoI GCNNNNNNNGC 7 cut(s) 195, 245, 268, 503, 512, 567, 573
NcoI CCATGG 1 cut(s) 571
NdeII GATC 2 cut(s) 715, 800
NlaIII CATG 8 cut(s) 239, 375, 418, 428, 575, 682, 725, 925
NlaIV GGNNCC 9 cut(s) 106, 115, 403, 454, 613, 660, 684, 751, 814
NmuCI GTSAC 1 cut(s) 697
NruI TCGCGA 1 cut(s) 930
NspI RCATGY 2 cut(s) 239, 725
PagI TCATGA 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 760
PfeI GAWTC 3 cut(s) 332, 849, 925
PkrI GCNGC 3 cut(s) 448, 505, 569
PleI GAGTC 1 cut(s) 840
PpsI GAGTC 1 cut(s) 840
PspEI GGTNACC 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 293
PspN4I GGNNCC 9 cut(s) 106, 115, 403, 454, 613, 660, 684, 751, 814
PspPI GGNCC 4 cut(s) 113, 659, 682, 776
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 1 cut(s) 800
RruI TCGCGA 1 cut(s) 930
RseI CAYNNNNRTG 1 cut(s) 920
SaqAI TTAA 2 cut(s) 242, 726
SatI GCNGC 3 cut(s) 447, 504, 568
Sau3AI GATC 2 cut(s) 715, 800
Sau96I GGNCC 4 cut(s) 113, 659, 682, 776
SchI GAGTC 1 cut(s) 841
SduI GDGCHC 4 cut(s) 300, 406, 754, 817
SfaNI GCATC 3 cut(s) 502, 573, 895
SfcI CTRYAG 2 cut(s) 408, 504
SinI GGWCC 2 cut(s) 682, 776
SmiMI CAYNNNNRTG 1 cut(s) 920
Sse9I AATT 4 cut(s) 468, 667, 729, 760
SsiI CCGC 1 cut(s) 635
SspMI CTAG 1 cut(s) 818
StyI CCWWGG 1 cut(s) 571
TaaI ACNGT 2 cut(s) 322, 608
TaqI TCGA 3 cut(s) 5, 277, 835
TasI AATT 4 cut(s) 468, 667, 729, 760
TfiI GAWTC 3 cut(s) 332, 849, 925
Tru1I TTAA 2 cut(s) 242, 726
Tru9I TTAA 2 cut(s) 242, 726
TscAI CASTG 3 cut(s) 513, 751, 922
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 3 cut(s) 446, 503, 567
Tsp45I GTSAC 1 cut(s) 697
TspDTI ATGAA 7 cut(s) 360, 431, 441, 617, 889, 938, 948
TspRI CASTG 3 cut(s) 513, 751, 922
VpaK11BI GGWCC 2 cut(s) 682, 776
XagI CCTNNNNNAGG 1 cut(s) 220
XapI RAATTY 1 cut(s) 729
XceI RCATGY 2 cut(s) 239, 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 505
XmnI GAANNNNTTC 1 cut(s) 760
XspI CTAG 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.