MD17G1048000.v1.1

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015677
Physical Location & Seq
Reverse (-)
3475769 .. 3479841
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1048000.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGGAAGCGCCACTCTCATCTCCACCTCCGCTGATGAATCGGAACCAGAGGTCGCCGTCTCCGCCGGCGACAACCATTGCCGATCTGAACGTGGATTCGCTGGCTCAGTGCGCCAGCTACCTCAGTCTCCAGGACCTCTCCAACTTCGCCATGACCTGCAACTATCTCAAAAAGGTCGCATATTCCGACTCCATCTGGCAGCGCTTCTACAGGGAGCGGTGGCCGCGGAAACCACCGTCCTCCGCCTCGCAAACGCCAGGAGTGAGGGAGGCCTATCTATCCATGCTTAAGGATTCGCTGCAATTCAAGTTTGCCGATCCGATTGTCGTCGATTTCTACACCGCTGCCAATTCAAATCGTTTGGATCACATATTATTGGACAAGAATGATATAATCTTCTCTCAGGGTTCACTGATACGAGTATTAACGATTGATCGTTGGTTAAACGGAACGGACTCGCCCCTCACTTTAATTGATCACAATGCAAGAATTACTTGCATGAGGTTGTTTCCGCTGAATGAAACATCTTTATATCGAAGCGAAACACAGAAAGAGGAGAATGCTTTGGTAACTTCAAGCGCTGACCATTCCATTCGGCTATGGTGGAAGGGTGCTTGCCGAAGATGTTTTAGAGGTCACAGTGGCCCAGTTTCAGCATTGTCAGATAAGTTGTTAGGTGATGGAGCAGGAAAAGTATTGGCAAGTGGAGGGGAAGATGGCACCGTTCGCCTTTGGTCTCTTAGCTCCAGTGGGAGACGTGGCCAGCATGCTTTAAAGGCTACTTTCTATGGGCATGAGAAGCCCATCCGATTGATGTCAGTTGCTGGGCACAATACCTCTCACTTGGTAACCGTTTCAAGAGATTCTAAGGTGAGGGTGTGGGATACAAATGCATCATCCTTTGCTCGTTCTTCATGCTGTGTGGGGATGACTTCTACTCTTGGTGCCCCTATAGACATGAAGTGTCATGAACATTTGGTCTATGTTGCTGCTGGTTCCTCCGTTACAGCAATTGATTTGAGGACAATGCGAAAAATTGTCATTGCTGCAGTGGACGCAGAGCTATATTCTTTTCAGGCAACGCCATCCAAATCTTTATTCTGCATAGGCAGCCATGGCAGAGCGATGCTTTACGATATAAGGATGAACTGTGGTACCCGGAAATCAGAACCAATAGCGGAGTTAGAAGCTGGACACAAAGGGCCGGTGCATTACTTGCACATGGACCCATACAAAATTGTCACCGGAAGCCCCAAGGATATGCATGTTAACATTTGGGAGGCTGACACTGGTGCCCTAACAAATTCCTTGAGCTGTGGTCCTGCAGGTGAAACAGACAGAAGCTCTTTGTTTTCTGCCCTAGCGGTAAATGGGAGTAGAATAGTTACTGGAATCGTCGATGTCCTCGATGATGTGGGATGTTTACGCTTTAGGGATTTCACAAATGCTTCTTGCCCTGTCGTGAATCGCGACAAAGATCATGTTGCAAAGTTTTGGAATCAGGAGTCGTACAGTGATACCGATGGTTCAGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

513

Amino Acids

56.37

Weight (kDa)

7.92

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 168 - 248 1.4e-07 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 181 - 300 2.4e-13 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 182 - 250 3e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 182 - 301 4.5e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 186 - 296 7.7e-07 WDR3 second beta-propeller domain
WD40 PF00400 205 - 246 1.3e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 206 - 302 3.9e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 229 - 436 2e-20 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 229 - 301 1.7e-07 WDR5 beta-propeller domain
WD40_Prp19 PF24814 230 - 427 5.4e-12 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 231 - 333 1.7e-06 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 277 - 442 4.8e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49170
malus_domestica MD09G1047900.v1.1 MD17G1048000.v1.1 MD17G1048100.v1.1
prunus_persica Prupe.3G271100_v2.0.a1
pyrus_communis pycom17g04280
rosa_chinensis RchiOBHm_Chr2g0169041
rosa_laevigata RLG00000021826
rosa_multiflora Rmu_ssc0000393.1_g000025
rosa_roxburghii Rroxscaffold_2G00082510
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG608000 Rh2BG620700 Rh2CG589600 Rh2DG631300
rosa_wichuraiana Rw2G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1316
Acc36I ACCTGC 2 cut(s) 164, 1316
Acc65I GGTACC 1 cut(s) 1154
AccB1I GGYRCC 4 cut(s) 719, 944, 1154, 1292
AccBSI CCGCTC 1 cut(s) 217
AccII CGCG 2 cut(s) 226, 1470
AclWI GGATC 2 cut(s) 311, 372
AcoI YGGCCR 2 cut(s) 221, 760
AcsI RAATTY 1 cut(s) 1303
AfaI GTAC 2 cut(s) 1156, 1511
AfeI AGCGCT 2 cut(s) 203, 580
AflII CTTAAG 1 cut(s) 287
AgsI TTSAA 4 cut(s) 307, 354, 576, 858
AjiI CACGTC 1 cut(s) 758
AjnI CCWGG 2 cut(s) 129, 256
AjuI GAANNNNNNNTTGG 2 cut(s) 1082, 1114
AluBI AGCT 6 cut(s) 117, 744, 1063, 1190, 1314, 1344
AluI AGCT 6 cut(s) 117, 744, 1063, 1190, 1314, 1344
Alw26I GTCTC 4 cut(s) 63, 131, 741, 748
AlwI GGATC 2 cut(s) 311, 372
AlwNI CAGNNNCTG 1 cut(s) 824
Aor51HI AGCGCT 2 cut(s) 203, 580
AoxI GGCC 5 cut(s) 221, 270, 643, 760, 1202
ApeKI GCWGC 6 cut(s) 199, 298, 344, 989, 1046, 1110
ApoI RAATTY 1 cut(s) 1303
Asp718I GGTACC 1 cut(s) 1154
AspLEI GCGC 4 cut(s) 10, 113, 204, 581
AspS9I GGNCC 5 cut(s) 133, 644, 1202, 1225, 1319
AsuC2I CCSGG 1 cut(s) 1159
AsuHPI GGTGA 4 cut(s) 689, 883, 1234, 1340
AvaII GGWCC 3 cut(s) 133, 1225, 1319
BaeGI GKGCMC 3 cut(s) 831, 949, 1297
BaeI ACNNNNGTAYC 2 cut(s) 1138, 1171
BalI TGGCCA 1 cut(s) 762
BanI GGYRCC 4 cut(s) 719, 944, 1154, 1292
BbvI GCAGC 6 cut(s) 211, 285, 331, 976, 1033, 1122
BccI CCATC 6 cut(s) 200, 674, 710, 812, 1093, 1517
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 2 cut(s) 131, 258
BciVI GTATCC 1 cut(s) 877
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 1 cut(s) 1159
BcoDI GTCTC 4 cut(s) 63, 131, 741, 748
BfaI CTAG 1 cut(s) 1361
BfmI CTRYAG 4 cut(s) 208, 951, 1047, 1323
BfoI RGCGCY 3 cut(s) 11, 205, 582
BfrI CTTAAG 1 cut(s) 287
BfuAI ACCTGC 2 cut(s) 164, 1316
BfuI GTATCC 1 cut(s) 877
BisI GCNGC 7 cut(s) 200, 224, 299, 345, 990, 1047, 1111
BlsI GCNGC 7 cut(s) 201, 225, 300, 346, 991, 1048, 1112
Bme1390I CCNGG 3 cut(s) 131, 258, 1159
Bme18I GGWCC 3 cut(s) 133, 1225, 1319
BmgBI CACGTC 1 cut(s) 758
BmgT120I GGNCC 5 cut(s) 133, 644, 1202, 1225, 1319
BmiI GGNNCC 7 cut(s) 44, 721, 946, 997, 1156, 1227, 1294
BmrFI CCNGG 3 cut(s) 131, 258, 1159
BmrI ACTGGG 1 cut(s) 641
BmsI GCATC 2 cut(s) 902, 1116
BmuI ACTGGG 1 cut(s) 641
BpmI CTGGAG 2 cut(s) 113, 730
BpuEI CTTGAG 1 cut(s) 1330
BpuMI CCSGG 1 cut(s) 1159
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 3 cut(s) 224, 1114, 1254
BsaWI WCCGGW 1 cut(s) 1244
Bse118I RCCGGY 2 cut(s) 64, 1204
Bse1I ACTGG 4 cut(s) 647, 747, 1294, 1393
Bse3DI GCAATG 2 cut(s) 75, 1041
BseBI CCWGG 2 cut(s) 131, 258
BseDI CCNNGG 3 cut(s) 224, 1114, 1254
BseGI GGATG 6 cut(s) 804, 896, 933, 1085, 1149, 1424
BseMI GCAATG 2 cut(s) 75, 1041
BseMII CTCAG 3 cut(s) 119, 136, 416
BseNI ACTGG 4 cut(s) 647, 747, 1294, 1393
BseRI GAGGAG 1 cut(s) 569
BseSI GKGCMC 3 cut(s) 831, 949, 1297
BseXI GCAGC 6 cut(s) 211, 285, 331, 976, 1033, 1122
BseYI CCCAGC 1 cut(s) 824
Bsh1236I CGCG 2 cut(s) 226, 1470
BshFI GGCC 5 cut(s) 223, 272, 645, 762, 1204
BshNI GGYRCC 4 cut(s) 719, 944, 1154, 1292
BsiSI CCGG 4 cut(s) 65, 1159, 1205, 1245
BsmAI GTCTC 4 cut(s) 63, 131, 741, 748
BsmBI CGTCTC 2 cut(s) 63, 748
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 5 cut(s) 223, 272, 645, 762, 1204
Bso31I GGTCTC 1 cut(s) 741
Bsp1286I GDGCHC 3 cut(s) 831, 949, 1297
Bsp143I GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
Bsp19I CCATGG 1 cut(s) 1114
Bsp68I TCGCGA 1 cut(s) 1470
BspANI GGCC 5 cut(s) 223, 272, 645, 762, 1204
BspCNI CTCAG 3 cut(s) 118, 135, 415
BspFNI CGCG 2 cut(s) 226, 1470
BspHI TCATGA 1 cut(s) 967
BspLI GGNNCC 7 cut(s) 44, 721, 946, 997, 1156, 1227, 1294
BspMAI CTGCAG 2 cut(s) 1051, 1327
BspMI ACCTGC 2 cut(s) 164, 1316
BspPI GGATC 2 cut(s) 311, 372
BspT107I GGYRCC 4 cut(s) 719, 944, 1154, 1292
BspTI CTTAAG 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 741
BsrBI CCGCTC 1 cut(s) 217
BsrDI GCAATG 2 cut(s) 75, 1041
BsrFI RCCGGY 2 cut(s) 64, 1204
BsrI ACTGG 4 cut(s) 647, 747, 1294, 1393
BssAI RCCGGY 2 cut(s) 64, 1204
BssECI CCNNGG 3 cut(s) 224, 1114, 1254
BssMI GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
BssT1I CCWWGG 2 cut(s) 1114, 1254
Bst2UI CCWGG 2 cut(s) 131, 258
Bst4CI ACNGT 6 cut(s) 237, 641, 724, 853, 1151, 1514
BstAFI CTTAAG 1 cut(s) 287
BstAPI GCANNNNNTGC 1 cut(s) 1216
BstC8I GCNNGC 6 cut(s) 66, 102, 115, 616, 764, 768
BstDEI CTNAG 5 cut(s) 105, 122, 402, 740, 867
BstDSI CCRYGG 2 cut(s) 224, 1114
BstEII GGTNACC 1 cut(s) 847
BstF5I GGATG 6 cut(s) 804, 896, 933, 1085, 1149, 1424
BstFNI CGCG 2 cut(s) 226, 1470
BstH2I RGCGCY 3 cut(s) 11, 205, 582
BstHHI GCGC 4 cut(s) 10, 113, 204, 581
BstKTI GATC 6 cut(s) 85, 319, 367, 436, 478, 1480
BstMAI GTCTC 4 cut(s) 63, 131, 741, 748
BstMBI GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
BstNI CCWGG 2 cut(s) 131, 258
BstNSI RCATGY 2 cut(s) 770, 1268
BstPI GGTNACC 1 cut(s) 847
BstSCI CCNGG 3 cut(s) 129, 256, 1157
BstSFI CTRYAG 4 cut(s) 208, 951, 1047, 1323
BstSLI GKGCMC 3 cut(s) 831, 949, 1297
BstUI CGCG 2 cut(s) 226, 1470
BstV1I GCAGC 6 cut(s) 211, 285, 331, 976, 1033, 1122
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 5 cut(s) 223, 272, 645, 762, 1204
BtgI CCRYGG 2 cut(s) 224, 1114
BtgZI GCGATG 1 cut(s) 1139
BtrI CACGTC 1 cut(s) 758
BtsCI GGATG 6 cut(s) 804, 896, 933, 1085, 1149, 1424
BtsI GCAGTG 1 cut(s) 1056
BtsIMutI CAGTG 7 cut(s) 113, 410, 646, 754, 1056, 1287, 1519
BtuMI TCGCGA 1 cut(s) 1470
BveI ACCTGC 2 cut(s) 164, 1316
Cac8I GCNNGC 6 cut(s) 66, 102, 115, 616, 764, 768
CaiI CAGNNNCTG 1 cut(s) 824
CciI TCATGA 1 cut(s) 967
CfoI GCGC 4 cut(s) 10, 113, 204, 581
Cfr10I RCCGGY 2 cut(s) 64, 1204
Cfr13I GGNCC 5 cut(s) 133, 644, 1202, 1225, 1319
Cfr42I CCGCGG 1 cut(s) 227
CseI GACGC 1 cut(s) 1064
Csp6I GTAC 2 cut(s) 1155, 1510
CviQI GTAC 2 cut(s) 1155, 1510
DdeI CTNAG 5 cut(s) 105, 122, 402, 740, 867
DpnI GATC 6 cut(s) 84, 318, 366, 435, 477, 1479
DpnII GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
DraI TTTAAA 1 cut(s) 774
EaeI YGGCCR 2 cut(s) 221, 760
EciI GGCGGA 2 cut(s) 51, 232
Eco130I CCWWGG 2 cut(s) 1114, 1254
Eco147I AGGCCT 1 cut(s) 272
Eco31I GGTCTC 1 cut(s) 741
Eco47I GGWCC 3 cut(s) 133, 1225, 1319
Eco47III AGCGCT 2 cut(s) 203, 580
Eco91I GGTNACC 1 cut(s) 847
EcoO109I RGGNCCY 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 847
EcoRII CCWGG 2 cut(s) 129, 256
EcoT14I CCWWGG 2 cut(s) 1114, 1254
EcoT22I ATGCAT 2 cut(s) 895, 1266
ErhI CCWWGG 2 cut(s) 1114, 1254
Esp3I CGTCTC 2 cut(s) 63, 748
FalI AAGNNNNNCTT 2 cut(s) 478, 510
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 7 cut(s) 200, 224, 299, 345, 990, 1047, 1111
FokI GGATG 6 cut(s) 791, 883, 940, 1072, 1156, 1431
Fsp4HI GCNGC 7 cut(s) 200, 224, 299, 345, 990, 1047, 1111
FspBI CTAG 1 cut(s) 1361
GlaI GCGC 4 cut(s) 9, 112, 203, 580
GluI GCNGC 7 cut(s) 200, 224, 299, 345, 990, 1047, 1111
GsaI CCCAGC 1 cut(s) 828
GsuI CTGGAG 2 cut(s) 113, 730
HaeII RGCGCY 3 cut(s) 11, 205, 582
HaeIII GGCC 5 cut(s) 223, 272, 645, 762, 1204
HapII CCGG 4 cut(s) 65, 1159, 1205, 1245
HgaI GACGC 1 cut(s) 1064
HhaI GCGC 4 cut(s) 10, 113, 204, 581
Hin6I GCGC 4 cut(s) 8, 111, 202, 579
HinP1I GCGC 4 cut(s) 8, 111, 202, 579
HincII GTYRAC 1 cut(s) 1270
HindII GTYRAC 1 cut(s) 1270
HpaI GTTAAC 1 cut(s) 1270
HpaII CCGG 4 cut(s) 65, 1159, 1205, 1245
HphI GGTGA 4 cut(s) 689, 883, 1234, 1340
Hpy166II GTNNAC 4 cut(s) 410, 1054, 1270, 1424
Hpy188I TCNGA 8 cut(s) 42, 87, 187, 321, 664, 809, 1168, 1531
Hpy188III TCNNGA 5 cut(s) 858, 968, 1462, 1469, 1502
Hpy8I GTNNAC 4 cut(s) 410, 1054, 1270, 1424
Hpy99I CGWCG 2 cut(s) 332, 1400
HpyAV CCTTC 1 cut(s) 601
HpyCH4III ACNGT 6 cut(s) 237, 641, 724, 853, 1151, 1514
HpyCH4IV ACGT 2 cut(s) 90, 757
HpyF3I CTNAG 5 cut(s) 105, 122, 402, 740, 867
HpySE526I ACGT 2 cut(s) 90, 757
HspAI GCGC 4 cut(s) 8, 111, 202, 579
KpnI GGTACC 1 cut(s) 1158
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Ksp22I TGATCA 1 cut(s) 475
KspAI GTTAAC 1 cut(s) 1270
KspI CCGCGG 1 cut(s) 227
Kzo9I GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
LmnI GCTCC 3 cut(s) 214, 683, 749
Lsp1109I GCAGC 6 cut(s) 211, 285, 331, 976, 1033, 1122
LweI GCATC 2 cut(s) 902, 1116
MaeI CTAG 1 cut(s) 1361
MaeII ACGT 2 cut(s) 90, 757
MaeIII GTNAC 6 cut(s) 568, 635, 847, 1003, 1240, 1384
MalI GATC 6 cut(s) 84, 318, 366, 435, 477, 1479
MbiI CCGCTC 1 cut(s) 217
MboI GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
MboII GAAGA 4 cut(s) 388, 633, 725, 903
MfeI CAATTG 1 cut(s) 1011
MhlI GDGCHC 3 cut(s) 831, 949, 1297
MlsI TGGCCA 1 cut(s) 762
MluCI AATT 8 cut(s) 302, 349, 471, 489, 1011, 1035, 1236, 1303
MluNI TGGCCA 1 cut(s) 762
MlyI GAGTC 3 cut(s) 182, 449, 1514
MmeI TCCRAC 2 cut(s) 165, 210
Mox20I TGGCCA 1 cut(s) 762
Mph1103I ATGCAT 2 cut(s) 895, 1266
MreI CGCCGGCG 1 cut(s) 64
MroNI GCCGGC 1 cut(s) 64
MscI TGGCCA 1 cut(s) 762
MseI TTAA 6 cut(s) 288, 425, 443, 470, 773, 1269
Msp20I TGGCCA 1 cut(s) 762
MspA1I CMGCKG 4 cut(s) 31, 226, 344, 514
MspCI CTTAAG 1 cut(s) 287
MspI CCGG 4 cut(s) 65, 1159, 1205, 1245
MspR9I CCNGG 3 cut(s) 131, 258, 1159
MunI CAATTG 1 cut(s) 1011
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 2 cut(s) 131, 258
MvnI CGCG 2 cut(s) 226, 1470
NaeI GCCGGC 1 cut(s) 66
NciI CCSGG 1 cut(s) 1159
NcoI CCATGG 1 cut(s) 1114
NdeII GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
NgoMIV GCCGGC 1 cut(s) 64
NlaIV GGNNCC 7 cut(s) 44, 721, 946, 997, 1156, 1227, 1294
NmuCI GTSAC 2 cut(s) 635, 1240
NruI TCGCGA 1 cut(s) 1470
NsiI ATGCAT 2 cut(s) 895, 1266
NspI RCATGY 2 cut(s) 770, 1268
PaeI GCATGC 1 cut(s) 770
PagI TCATGA 1 cut(s) 967
PaqCI CACCTGC 1 cut(s) 1316
PceI AGGCCT 1 cut(s) 272
PcsI WCGNNNNNNNCGW 3 cut(s) 183, 1404, 1467
PctI GAATGC 1 cut(s) 565
PdiI GCCGGC 1 cut(s) 66
PfeI GAWTC 7 cut(s) 37, 95, 293, 863, 1392, 1465, 1498
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 7 cut(s) 201, 225, 300, 346, 991, 1048, 1112
PleI GAGTC 3 cut(s) 182, 449, 1513
PpsI GAGTC 3 cut(s) 182, 449, 1513
PpuMI RGGWCCY 1 cut(s) 133
Psp5II RGGWCCY 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 129, 256
PspEI GGTNACC 1 cut(s) 847
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 2 cut(s) 129, 256
PspN4I GGNNCC 7 cut(s) 44, 721, 946, 997, 1156, 1227, 1294
PspPI GGNCC 5 cut(s) 133, 644, 1202, 1225, 1319
PspPPI RGGWCCY 1 cut(s) 133
PstI CTGCAG 2 cut(s) 1051, 1327
PstNI CAGNNNCTG 1 cut(s) 824
RruI TCGCGA 1 cut(s) 1470
RsaI GTAC 2 cut(s) 1156, 1511
RsaNI GTAC 2 cut(s) 1155, 1510
SacII CCGCGG 1 cut(s) 227
SaqAI TTAA 6 cut(s) 288, 425, 443, 470, 773, 1269
SatI GCNGC 7 cut(s) 200, 224, 299, 345, 990, 1047, 1111
Sau3AI GATC 6 cut(s) 82, 316, 364, 433, 475, 1477
Sau96I GGNCC 5 cut(s) 133, 644, 1202, 1225, 1319
SbfI CCTGCAGG 1 cut(s) 1327
SchI GAGTC 3 cut(s) 182, 449, 1514
ScrFI CCNGG 3 cut(s) 131, 258, 1159
SdaI CCTGCAGG 1 cut(s) 1327
SduI GDGCHC 3 cut(s) 831, 949, 1297
SfaNI GCATC 2 cut(s) 902, 1116
SfcI CTRYAG 4 cut(s) 208, 951, 1047, 1323
Sfr303I CCGCGG 1 cut(s) 227
SgrAI CRCCGGYG 1 cut(s) 64
SgrBI CCGCGG 1 cut(s) 227
SinI GGWCC 3 cut(s) 133, 1225, 1319
SmlI CTYRAG 2 cut(s) 287, 1309
SmoI CTYRAG 2 cut(s) 287, 1309
SphI GCATGC 1 cut(s) 770
Sse8387I CCTGCAGG 1 cut(s) 1327
Sse9I AATT 8 cut(s) 302, 349, 471, 489, 1011, 1035, 1236, 1303
SseBI AGGCCT 1 cut(s) 272
SspMI CTAG 1 cut(s) 1361
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 129, 256, 1157
StyI CCWWGG 2 cut(s) 1114, 1254
TaaI ACNGT 6 cut(s) 237, 641, 724, 853, 1151, 1514
TaiI ACGT 2 cut(s) 93, 760
TaqI TCGA 4 cut(s) 330, 535, 1398, 1407
TasI AATT 8 cut(s) 302, 349, 471, 489, 1011, 1035, 1236, 1303
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 7 cut(s) 37, 95, 293, 863, 1392, 1465, 1498
Tru1I TTAA 6 cut(s) 288, 425, 443, 470, 773, 1269
Tru9I TTAA 6 cut(s) 288, 425, 443, 470, 773, 1269
TscAI CASTG 7 cut(s) 113, 417, 646, 754, 1056, 1294, 1519
TseFI GTSAC 2 cut(s) 635, 1240
TseI GCWGC 6 cut(s) 199, 298, 344, 989, 1046, 1110
Tsp45I GTSAC 2 cut(s) 635, 1240
TspDTI ATGAA 6 cut(s) 50, 534, 903, 974, 984, 1160
TspGWI ACGGA 3 cut(s) 462, 467, 991
TspRI CASTG 7 cut(s) 113, 417, 646, 754, 1056, 1294, 1519
Vha464I CTTAAG 1 cut(s) 287
VpaK11BI GGWCC 3 cut(s) 133, 1225, 1319
XapI RAATTY 1 cut(s) 1303
XceI RCATGY 2 cut(s) 770, 1268
XspI CTAG 1 cut(s) 1361
Zsp2I ATGCAT 2 cut(s) 895, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.