pycom17g04280

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0015677
Physical Location & Seq
Reverse (-)
2921661 .. 2923744
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g04280.1

Sequence Viewer

Length: 1041 bp
ATGAGGTTGTTTCCGCTGAATGAAACATCTTTATATCGAAGCGAAACACAGAAAGAGGAGAATGCTTTGGTAACTTCAAGCGCTGACCATTCCATTCGGCTATGGTGGAAGGGTGCTTGCCGAAGATGTTTTAGAGGTCACAGTGGCCCAGTTTCAGCATTGTCAGATAAGTTGTTAGGCGATGGAGCAGGAAAAGTATTGGCAAGTGGAGGGGAAGATGGCACCGTTCGCCTTTGGTCTCTTAGCTCCGGTGGGAGACGTGGCCAGCATGCTTTAAAGGCTACTTTCTATGGGCATGAGAAGCCCATCCGATTGATGTCAGTTGCTGGGCACAACACCTCTCACTTGGTAACTGTTTCAAGAGATTCTAAGGTGAGGGTGTGGGATACAAATGCTTCATCCTTTGCTTGTTCTTCATGCTGTGTGGGGATGACTTCTACTGTTGGTGCCCCTATAGACATGAAGTGTCATGAACATTTGGTCTATGTTGCTGCTGGTTCCTCCGTTATGGCAATTGATTTGAGGACAATGCAAAAAGTTGTCATTGCTGCAGTGGATGCAAAGCTATATTCTTTTCAGGCAAGGCCATCCAAATCTTTATTCTGCATAGGCAGCCATGGCAGAGCGATGCTTTACGATTTAAGGATGAACTGTGGTACCCTGAAATCAGAACCAGTAGCGGAGTTAGAAGCTGGACACAAAGGGCCGGTGAATTACTTGCACATGGACCCATACAAAATTGTCACCGGAAGCCCCAAGGATATGCATGTTAACATTTGGGAGGCTGACACTGGTGCCCTAACAAATTCCTTGAGCTGTGGTCCTGCAGGTGAAACAGACAGAAGCTCTGTGTTTTCTGCCCTAGCAGTAAATGGGAGTCGAATAGTTACTGGAATCGTCAATGTCCTCTATGATGTGGGATGTTTACGCTTTAGGGATTTCACAAATGCTTCTTGCCCTGTCGTGAATCGCGACAAGGGTCATGTTGCAAAGTTTTGGAATCAAGAGTCTTACAGTGATACCGATAGTTCAGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

347

Amino Acids

37.5

Weight (kDa)

8.44

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 16 - 162 2.7e-14 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 16 - 84 1.7e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 18 - 84 4.9e-08 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 20 - 134 1.8e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 20 - 130 4.1e-07 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 23 - 130 2.5e-06 SCAP Beta-propeller
WD40 PF00400 39 - 80 7.7e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 63 - 271 6.9e-20 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 63 - 148 4.2e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 64 - 261 2.4e-13 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 65 - 168 8.4e-07 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 111 - 276 3.1e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 113 - 273 6.4e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49170
malus_domestica MD09G1047900.v1.1 MD17G1048000.v1.1 MD17G1048100.v1.1
prunus_persica Prupe.3G271100_v2.0.a1
pyrus_communis pycom17g04280
rosa_chinensis RchiOBHm_Chr2g0169041
rosa_laevigata RLG00000021826
rosa_multiflora Rmu_ssc0000393.1_g000025
rosa_roxburghii Rroxscaffold_2G00082510
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG608000 Rh2BG620700 Rh2CG589600 Rh2DG631300
rosa_wichuraiana Rw2G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 818
Acc36I ACCTGC 1 cut(s) 818
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 4 cut(s) 221, 446, 656, 794
AccII CGCG 1 cut(s) 972
AciI CCGC 2 cut(s) 14, 680
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 805
AfaI GTAC 1 cut(s) 658
AfeI AGCGCT 1 cut(s) 82
AgsI TTSAA 2 cut(s) 78, 360
AjiI CACGTC 1 cut(s) 260
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AluBI AGCT 5 cut(s) 246, 565, 692, 816, 846
AluI AGCT 5 cut(s) 246, 565, 692, 816, 846
Alw26I GTCTC 2 cut(s) 243, 250
AlwNI CAGNNNCTG 1 cut(s) 326
Aor51HI AGCGCT 1 cut(s) 82
AoxI GGCC 4 cut(s) 145, 262, 584, 704
ApeKI GCWGC 3 cut(s) 491, 548, 612
ApoI RAATTY 1 cut(s) 805
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 4 cut(s) 146, 704, 727, 821
AsuHPI GGTGA 4 cut(s) 385, 721, 736, 842
AvaII GGWCC 2 cut(s) 727, 821
BaeGI GKGCMC 3 cut(s) 333, 451, 799
BaeI ACNNNNGTAYC 2 cut(s) 640, 673
BalI TGGCCA 1 cut(s) 264
BanI GGYRCC 4 cut(s) 221, 446, 656, 794
BbvI GCAGC 3 cut(s) 478, 535, 624
BccI CCATC 4 cut(s) 176, 212, 314, 595
BciVI GTATCC 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 243, 250
BfaI CTAG 1 cut(s) 863
BfmI CTRYAG 3 cut(s) 453, 549, 825
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 818
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 3 cut(s) 492, 549, 613
BlsI GCNGC 3 cut(s) 493, 550, 614
Bme18I GGWCC 2 cut(s) 727, 821
BmgBI CACGTC 1 cut(s) 260
BmgT120I GGNCC 4 cut(s) 146, 704, 727, 821
BmiI GGNNCC 6 cut(s) 223, 448, 499, 658, 729, 796
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 547, 618
BmuI ACTGGG 1 cut(s) 143
BoxI GACNNNNGTC 1 cut(s) 978
BpuEI CTTGAG 1 cut(s) 832
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 2 cut(s) 616, 756
BsaWI WCCGGW 2 cut(s) 248, 746
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 4 cut(s) 149, 674, 796, 895
Bse3DI GCAATG 1 cut(s) 543
BseDI CCNNGG 2 cut(s) 616, 756
BseGI GGATG 7 cut(s) 306, 398, 435, 562, 587, 651, 926
BseMI GCAATG 1 cut(s) 543
BseNI ACTGG 4 cut(s) 149, 674, 796, 895
BseRI GAGGAG 1 cut(s) 71
BseSI GKGCMC 3 cut(s) 333, 451, 799
BseXI GCAGC 3 cut(s) 478, 535, 624
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 972
BshFI GGCC 4 cut(s) 147, 264, 586, 706
BshNI GGYRCC 4 cut(s) 221, 446, 656, 794
BsiSI CCGG 3 cut(s) 249, 707, 747
BsmAI GTCTC 2 cut(s) 243, 250
BsmBI CGTCTC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 4 cut(s) 147, 264, 586, 706
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 3 cut(s) 333, 451, 799
Bsp19I CCATGG 1 cut(s) 616
Bsp68I TCGCGA 1 cut(s) 972
BspACI CCGC 2 cut(s) 14, 680
BspANI GGCC 4 cut(s) 147, 264, 586, 706
BspFNI CGCG 1 cut(s) 972
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 6 cut(s) 223, 448, 499, 658, 729, 796
BspMAI CTGCAG 2 cut(s) 553, 829
BspMI ACCTGC 1 cut(s) 818
BspT107I GGYRCC 4 cut(s) 221, 446, 656, 794
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 706
BsrI ACTGG 4 cut(s) 149, 674, 796, 895
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 2 cut(s) 616, 756
BssT1I CCWWGG 2 cut(s) 616, 756
Bst4CI ACNGT 6 cut(s) 143, 226, 355, 442, 653, 1016
BstAPI GCANNNNNTGC 1 cut(s) 557
BstC8I GCNNGC 3 cut(s) 118, 266, 270
BstDEI CTNAG 2 cut(s) 242, 369
BstDSI CCRYGG 1 cut(s) 616
BstF5I GGATG 7 cut(s) 306, 398, 435, 562, 587, 651, 926
BstFNI CGCG 1 cut(s) 972
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstMAI GTCTC 2 cut(s) 243, 250
BstMWI GCNNNNNNNGC 6 cut(s) 228, 278, 301, 557, 612, 618
BstNSI RCATGY 2 cut(s) 272, 770
BstPAI GACNNNNGTC 1 cut(s) 978
BstSFI CTRYAG 3 cut(s) 453, 549, 825
BstSLI GKGCMC 3 cut(s) 333, 451, 799
BstUI CGCG 1 cut(s) 972
BstV1I GCAGC 3 cut(s) 478, 535, 624
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 4 cut(s) 147, 264, 586, 706
BtgI CCRYGG 1 cut(s) 616
BtgZI GCGATG 2 cut(s) 195, 641
BtrI CACGTC 1 cut(s) 260
BtsCI GGATG 7 cut(s) 306, 398, 435, 562, 587, 651, 926
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 4 cut(s) 148, 558, 789, 1021
BtuMI TCGCGA 1 cut(s) 972
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 3 cut(s) 118, 266, 270
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 469
CfoI GCGC 1 cut(s) 83
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 4 cut(s) 146, 704, 727, 821
Csp6I GTAC 1 cut(s) 657
CviAII CATG 9 cut(s) 269, 296, 417, 460, 470, 617, 724, 767, 983
CviQI GTAC 1 cut(s) 657
DdeI CTNAG 2 cut(s) 242, 369
DraI TTTAAA 1 cut(s) 276
EaeI YGGCCR 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 616, 756
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 727, 821
Eco47III AGCGCT 1 cut(s) 82
EcoT14I CCWWGG 2 cut(s) 616, 756
EcoT22I ATGCAT 1 cut(s) 768
ErhI CCWWGG 2 cut(s) 616, 756
Esp3I CGTCTC 1 cut(s) 250
FaeI CATG 9 cut(s) 272, 299, 420, 463, 473, 620, 727, 770, 986
FatI CATG 9 cut(s) 268, 295, 416, 459, 469, 616, 723, 766, 982
Fnu4HI GCNGC 3 cut(s) 492, 549, 613
FokI GGATG 7 cut(s) 293, 385, 442, 569, 574, 658, 933
Fsp4HI GCNGC 3 cut(s) 492, 549, 613
FspBI CTAG 1 cut(s) 863
GlaI GCGC 1 cut(s) 82
GluI GCNGC 3 cut(s) 492, 549, 613
GsaI CCCAGC 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 4 cut(s) 147, 264, 586, 706
HapII CCGG 3 cut(s) 249, 707, 747
HhaI GCGC 1 cut(s) 83
Hin1II CATG 9 cut(s) 272, 299, 420, 463, 473, 620, 727, 770, 986
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 6 cut(s) 365, 877, 894, 967, 1000, 1007
HpaI GTTAAC 1 cut(s) 772
HpaII CCGG 3 cut(s) 249, 707, 747
HphI GGTGA 4 cut(s) 385, 721, 736, 842
Hpy166II GTNNAC 2 cut(s) 772, 926
Hpy188I TCNGA 4 cut(s) 166, 311, 670, 1033
Hpy188III TCNNGA 5 cut(s) 360, 470, 964, 971, 1004
Hpy8I GTNNAC 2 cut(s) 772, 926
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 6 cut(s) 143, 226, 355, 442, 653, 1016
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 8 cut(s) 532, 551, 560, 606, 721, 766, 827, 989
HpyF10VI GCNNNNNNNGC 6 cut(s) 228, 278, 301, 557, 612, 618
HpyF3I CTNAG 2 cut(s) 242, 369
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 9 cut(s) 272, 299, 420, 463, 473, 620, 727, 770, 986
HspAI GCGC 1 cut(s) 81
KpnI GGTACC 1 cut(s) 660
KspAI GTTAAC 1 cut(s) 772
LmnI GCTCC 2 cut(s) 185, 251
Lsp1109I GCAGC 3 cut(s) 478, 535, 624
LweI GCATC 2 cut(s) 547, 618
MaeI CTAG 1 cut(s) 863
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 5 cut(s) 70, 137, 349, 742, 886
MboII GAAGA 3 cut(s) 135, 227, 405
MfeI CAATTG 1 cut(s) 513
MhlI GDGCHC 3 cut(s) 333, 451, 799
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 4 cut(s) 513, 712, 738, 805
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 2 cut(s) 886, 1016
MnlI CCTC 9 cut(s) 49, 128, 203, 349, 369, 511, 516, 775, 917
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 1 cut(s) 768
MscI TGGCCA 1 cut(s) 264
MseI TTAA 3 cut(s) 275, 641, 771
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 3 cut(s) 249, 707, 747
MunI CAATTG 1 cut(s) 513
Mva1269I GAATGC 1 cut(s) 67
MvnI CGCG 1 cut(s) 972
MwoI GCNNNNNNNGC 6 cut(s) 228, 278, 301, 557, 612, 618
NcoI CCATGG 1 cut(s) 616
NlaIII CATG 9 cut(s) 272, 299, 420, 463, 473, 620, 727, 770, 986
NlaIV GGNNCC 6 cut(s) 223, 448, 499, 658, 729, 796
NmuCI GTSAC 2 cut(s) 137, 742
NruI TCGCGA 1 cut(s) 972
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 2 cut(s) 272, 770
PaeI GCATGC 1 cut(s) 272
PagI TCATGA 1 cut(s) 469
PaqCI CACCTGC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 969
PctI GAATGC 1 cut(s) 67
PfeI GAWTC 4 cut(s) 365, 894, 967, 1000
PkrI GCNGC 3 cut(s) 493, 550, 614
PleI GAGTC 2 cut(s) 885, 1015
PpsI GAGTC 2 cut(s) 885, 1015
PshAI GACNNNNGTC 1 cut(s) 978
PspFI CCCAGC 1 cut(s) 326
PspN4I GGNNCC 6 cut(s) 223, 448, 499, 658, 729, 796
PspPI GGNCC 4 cut(s) 146, 704, 727, 821
PstI CTGCAG 2 cut(s) 553, 829
PstNI CAGNNNCTG 1 cut(s) 326
RruI TCGCGA 1 cut(s) 972
RsaI GTAC 1 cut(s) 658
RsaNI GTAC 1 cut(s) 657
SaqAI TTAA 3 cut(s) 275, 641, 771
SatI GCNGC 3 cut(s) 492, 549, 613
Sau96I GGNCC 4 cut(s) 146, 704, 727, 821
SbfI CCTGCAGG 1 cut(s) 829
SchI GAGTC 2 cut(s) 886, 1016
SdaI CCTGCAGG 1 cut(s) 829
SduI GDGCHC 3 cut(s) 333, 451, 799
SfaNI GCATC 2 cut(s) 547, 618
SfcI CTRYAG 3 cut(s) 453, 549, 825
SinI GGWCC 2 cut(s) 727, 821
SmlI CTYRAG 1 cut(s) 811
SmoI CTYRAG 1 cut(s) 811
SphI GCATGC 1 cut(s) 272
Sse8387I CCTGCAGG 1 cut(s) 829
Sse9I AATT 4 cut(s) 513, 712, 738, 805
SsiI CCGC 2 cut(s) 14, 680
SspMI CTAG 1 cut(s) 863
StyI CCWWGG 2 cut(s) 616, 756
TaaI ACNGT 6 cut(s) 143, 226, 355, 442, 653, 1016
TaiI ACGT 1 cut(s) 262
TaqI TCGA 2 cut(s) 37, 880
TasI AATT 4 cut(s) 513, 712, 738, 805
TfiI GAWTC 4 cut(s) 365, 894, 967, 1000
Tru1I TTAA 3 cut(s) 275, 641, 771
Tru9I TTAA 3 cut(s) 275, 641, 771
TscAI CASTG 4 cut(s) 148, 558, 796, 1021
TseFI GTSAC 2 cut(s) 137, 742
TseI GCWGC 3 cut(s) 491, 548, 612
Tsp45I GTSAC 2 cut(s) 137, 742
TspDTI ATGAA 6 cut(s) 36, 387, 405, 476, 486, 662
TspGWI ACGGA 1 cut(s) 493
TspRI CASTG 4 cut(s) 148, 558, 796, 1021
VpaK11BI GGWCC 2 cut(s) 727, 821
XapI RAATTY 1 cut(s) 805
XceI RCATGY 2 cut(s) 272, 770
XspI CTAG 1 cut(s) 863
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.