Prupe.3G271100_v2.0.a1

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0015677
Physical Location & Seq
Forward (+)
25203562 .. 25206298
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G271100.1

Sequence Viewer

Length: 1527 bp
ATGGAAGCGCCATCATCGTCATCTTCGCCGATGAGTGAGAACCAGAGGCCGTCGTTACCGGCAACAAGCATCACTGATGTGAACGTCGACTCGCTGGCTCAATGCGCCGGCTACCTCAGCCTCCGAGACCTCTCCAACATGTCCATGACCTGCACGTACCTCAAAAAGGTTGCATATTCCGACTCCATCTGGCAACGTTGGTTCAGAGAAAGCTGGCCCCGGCAAACACCTCCCGCCACCTCGCAAACGGCAGGAGTGAGGGAAGCTTATTTGTGTAGGCGCAAGGATTTGCTGCAATTCAAGTTTATCAATCCCCTCGTCGCCGATTTCTATACCAATTCAAGTCCTTTCGACCATGTGTTATTGGACAATAACAATATAATCTTCTCTCAGGGCTCAATGATACAAGTGATAACGATTGATAGCTGGTTAAGTGGAGTAAACTCACCTGTCACTTTAAGTGATCACAATGCAAGAATTACTTGCATGAGGCTGTTTCCACTGAATGAAACATCTTTATATCGAAGGGAAGCACAAAACGAGGAGAATGTTTTGGTAACTTCAAGCTCTGACCATTCCATTCGGCTATGGTGGAAGGGTTCTTGCCGACGATGTTTTAGAGGTCACAGTGGCCCAGTTTCAGCCCTGTCAGATAAATTGTTAGGCAATGGAGCAGGAAAAGTATTGGCAAGTGGAGGGGAAGATGGCACAGTTCGCCTTTGGTCTCTAAGTTCCAGTGGGAAACGTGGCCAGCATGCTTTAAAGGCTACTTTCTATGGGCATGAGAAGCCCATCCGGTTGATGTCAGTTGCTGGGCACAACACCTCTCTCTTGGTATCCGTTTCAAGAGATTCTAAGGTGAGGGTGTGGGATACAAATACATCATCCTCTGTTCGTTCTTCATGCTGTGTGGGGATGACCTCTGTTCTTGGTGCCCCTATAGACATTAAGTGTCATGAACAATTGGTCTATGTTGCTGCTGGTTCCTCTGTTATGGCAATTGATTTGAGGACAATGCAAAAAGTTGTCATTGCTACAGTAGATGCAAAGCTGTACTCTTTTCAGGCAACGCCTTCAAAATCTTTATTCTGTGTAGGCAGCAATGGCAGGGCAATGCTTTATGATATAAGGAAGAACCAGGGAACCCTGAAATCAGAACCAATAGCGGAGTTAGATGGTGGACACACAGCGCCGTTGACATACTTGCACATGGATCCATACAAAATAGTTACAGGAAGCCCGGAGGATTTTTATGTTAATGTTTGGGAGGCAAACACTGGTGCAAAAACTAATTCCTTGGATTGTTCTCATCCAGATGAAACATCTACCAGTTTTGGGTGTCGTGCTCTGGCTGTAAATGGGTGTCGAATTGTTACTGGCATTGCCAACTATGATGAAGATGTGGGATGCTTATGCTTCAGGGACTTCACAAATGCTTCTTGTCCTGTCTTTAATCGTGAAGATGATCATGTTGCAAAGTTTTGGAACCCACAATCTTACAGTGATTCCGATAGTTCATATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box WD40

Protein Analysis

509

Amino Acids

55.93

Weight (kDa)

6.85

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g49170
malus_domestica MD09G1047900.v1.1 MD17G1048000.v1.1 MD17G1048100.v1.1
prunus_persica Prupe.3G271100_v2.0.a1
pyrus_communis pycom17g04280
rosa_chinensis RchiOBHm_Chr2g0169041
rosa_laevigata RLG00000021826
rosa_multiflora Rmu_ssc0000393.1_g000025
rosa_roxburghii Rroxscaffold_2G00082510
rosa_rugosa Rorug02G0536500
rosa_samantha Rh2AG608000 Rh2BG620700 Rh2CG589600 Rh2DG631300
rosa_wichuraiana Rw2G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 932
AccI GTMKAC 1 cut(s) 87
AciI CCGC 2 cut(s) 234, 1166
AclI AACGTT 1 cut(s) 196
AclWI GGATC 2 cut(s) 1208, 1221
AcoI YGGCCR 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 1402
AfaI GTAC 2 cut(s) 158, 1055
AfiI CCNNNNNNNGG 2 cut(s) 166, 1335
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 5 cut(s) 301, 342, 564, 846, 1077
AjnI CCWGG 1 cut(s) 1137
AleI CACNNNNGTG 1 cut(s) 77
AluBI AGCT 5 cut(s) 213, 266, 426, 567, 1051
AluI AGCT 5 cut(s) 213, 266, 426, 567, 1051
Alw21I GWGCWC 1 cut(s) 1348
Alw26I GTCTC 2 cut(s) 120, 729
AlwI GGATC 2 cut(s) 1208, 1221
AlwNI CAGNNNCTG 1 cut(s) 812
AoxI GGCC 4 cut(s) 47, 215, 631, 748
ApeKI GCWGC 3 cut(s) 292, 977, 1098
AspLEI GCGC 4 cut(s) 10, 107, 282, 1192
AspS9I GGNCC 2 cut(s) 216, 632
AsuC2I CCSGG 2 cut(s) 220, 1241
AsuHPI GGTGA 2 cut(s) 438, 871
BaeGI GKGCMC 2 cut(s) 819, 937
BalI TGGCCA 1 cut(s) 750
BamHI GGATCC 1 cut(s) 1213
BanI GGYRCC 1 cut(s) 932
BanII GRGCYC 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 1348
BbvCI CCTCAGC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 279, 964, 1110
BccI CCATC 5 cut(s) 19, 194, 698, 800, 1169
BceAI ACGGC 3 cut(s) 34, 264, 1177
BciT130I CCWGG 1 cut(s) 1139
BciVI GTATCC 2 cut(s) 847, 865
BclI TGATCA 2 cut(s) 463, 1465
BcnI CCSGG 2 cut(s) 220, 1241
BcoDI GTCTC 2 cut(s) 120, 729
BfmI CTRYAG 2 cut(s) 939, 1035
BfoI RGCGCY 2 cut(s) 11, 1193
BfuAI ACCTGC 1 cut(s) 158
BfuI GTATCC 2 cut(s) 847, 865
BisI GCNGC 3 cut(s) 293, 978, 1099
BlsI GCNGC 3 cut(s) 294, 979, 1100
Bme1390I CCNGG 3 cut(s) 220, 1139, 1241
BmgT120I GGNCC 2 cut(s) 216, 632
BmiI GGNNCC 6 cut(s) 218, 934, 985, 1144, 1215, 1487
BmrFI CCNGG 3 cut(s) 220, 1139, 1241
BmrI ACTGGG 1 cut(s) 629
BmsI GCATC 3 cut(s) 78, 1033, 1397
BmuI ACTGGG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 116
BpuMI CCSGG 2 cut(s) 220, 1241
BsaAI YACGTR 1 cut(s) 156
BsaI GGTCTC 2 cut(s) 120, 729
BsaJI CCNNGG 3 cut(s) 218, 1138, 1296
BsaWI WCCGGW 1 cut(s) 795
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 1335
Bse118I RCCGGY 2 cut(s) 58, 107
Bse1I ACTGG 5 cut(s) 635, 735, 1282, 1329, 1381
Bse3DI GCAATG 5 cut(s) 673, 1029, 1108, 1119, 1380
BseBI CCWGG 1 cut(s) 1139
BseDI CCNNGG 3 cut(s) 218, 1138, 1296
BseGI GGATG 5 cut(s) 792, 884, 921, 1309, 1412
BseLI CCNNNNNNNGG 2 cut(s) 166, 1335
BseMI GCAATG 5 cut(s) 673, 1029, 1108, 1119, 1380
BseMII CTCAG 2 cut(s) 130, 404
BseNI ACTGG 5 cut(s) 635, 735, 1282, 1329, 1381
BseRI GAGGAG 1 cut(s) 557
BseSI GKGCMC 2 cut(s) 819, 937
BseXI GCAGC 3 cut(s) 279, 964, 1110
BseYI CCCAGC 1 cut(s) 812
BsgI GTGCAG 1 cut(s) 136
BshFI GGCC 4 cut(s) 49, 217, 633, 750
BshNI GGYRCC 1 cut(s) 932
BsiHKAI GWGCWC 1 cut(s) 1348
BsiSI CCGG 5 cut(s) 59, 108, 220, 796, 1241
BslFI GGGAC 1 cut(s) 1436
BslI CCNNNNNNNGG 2 cut(s) 166, 1335
BsmAI GTCTC 2 cut(s) 120, 729
BsmFI GGGAC 1 cut(s) 1436
BsnI GGCC 4 cut(s) 49, 217, 633, 750
Bso31I GGTCTC 2 cut(s) 120, 729
Bsp1286I GDGCHC 4 cut(s) 398, 819, 937, 1348
Bsp143I GATC 3 cut(s) 463, 1213, 1465
BspACI CCGC 2 cut(s) 234, 1166
BspANI GGCC 4 cut(s) 49, 217, 633, 750
BspCNI CTCAG 2 cut(s) 129, 403
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 6 cut(s) 218, 934, 985, 1144, 1215, 1487
BspMI ACCTGC 1 cut(s) 158
BspPI GGATC 2 cut(s) 1208, 1221
BspT107I GGYRCC 1 cut(s) 932
BspTNI GGTCTC 2 cut(s) 120, 729
BsrDI GCAATG 5 cut(s) 673, 1029, 1108, 1119, 1380
BsrFI RCCGGY 2 cut(s) 58, 107
BsrI ACTGG 5 cut(s) 635, 735, 1282, 1329, 1381
BssAI RCCGGY 2 cut(s) 58, 107
BssECI CCNNGG 3 cut(s) 218, 1138, 1296
BssMI GATC 3 cut(s) 463, 1213, 1465
BssT1I CCWWGG 1 cut(s) 1296
Bst2UI CCWGG 1 cut(s) 1139
Bst4CI ACNGT 4 cut(s) 629, 712, 1039, 1502
BstBAI YACGTR 1 cut(s) 156
BstC8I GCNNGC 5 cut(s) 96, 109, 215, 752, 756
BstDEI CTNAG 4 cut(s) 116, 390, 728, 855
BstENI CCTNNNNNAGG 1 cut(s) 164
BstF5I GGATG 5 cut(s) 792, 884, 921, 1309, 1412
BstH2I RGCGCY 2 cut(s) 11, 1193
BstHHI GCGC 4 cut(s) 10, 107, 282, 1192
BstKTI GATC 3 cut(s) 466, 1216, 1468
BstMAI GTCTC 2 cut(s) 120, 729
BstMBI GATC 3 cut(s) 463, 1213, 1465
BstMWI GCNNNNNNNGC 6 cut(s) 104, 117, 714, 764, 787, 1104
BstNI CCWGG 1 cut(s) 1139
BstNSI RCATGY 2 cut(s) 142, 758
BstSCI CCNGG 3 cut(s) 218, 1137, 1239
BstSFI CTRYAG 2 cut(s) 939, 1035
BstSLI GKGCMC 2 cut(s) 819, 937
BstV1I GCAGC 3 cut(s) 279, 964, 1110
BstX2I RGATCY 1 cut(s) 1213
BstYI RGATCY 1 cut(s) 1213
BsuI GTATCC 2 cut(s) 847, 865
BsuRI GGCC 4 cut(s) 49, 217, 633, 750
BtsCI GGATG 5 cut(s) 792, 884, 921, 1309, 1412
BtsIMutI CAGTG 6 cut(s) 72, 500, 634, 742, 1275, 1507
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 5 cut(s) 96, 109, 215, 752, 756
CaiI CAGNNNCTG 1 cut(s) 812
CciI TCATGA 1 cut(s) 955
CfoI GCGC 4 cut(s) 10, 107, 282, 1192
Cfr10I RCCGGY 2 cut(s) 58, 107
Cfr13I GGNCC 2 cut(s) 216, 632
Csp6I GTAC 2 cut(s) 157, 1054
CviQI GTAC 2 cut(s) 157, 1054
DdeI CTNAG 4 cut(s) 116, 390, 728, 855
DpnI GATC 3 cut(s) 465, 1215, 1467
DpnII GATC 3 cut(s) 463, 1213, 1465
DraI TTTAAA 1 cut(s) 762
EaeI YGGCCR 1 cut(s) 748
Eco130I CCWWGG 1 cut(s) 1296
Eco24I GRGCYC 1 cut(s) 398
Eco31I GGTCTC 2 cut(s) 120, 729
Eco57I CTGAAG 1 cut(s) 1402
EcoNI CCTNNNNNAGG 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 1137
EcoT14I CCWWGG 1 cut(s) 1296
EcoT38I GRGCYC 1 cut(s) 398
ErhI CCWWGG 1 cut(s) 1296
FalI AAGNNNNNCTT 2 cut(s) 466, 498
FaqI GGGAC 1 cut(s) 1436
FauI CCCGC 1 cut(s) 241
FauNDI CATATG 1 cut(s) 1519
FbaI TGATCA 2 cut(s) 463, 1465
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 3 cut(s) 293, 978, 1099
FokI GGATG 5 cut(s) 779, 871, 928, 1296, 1419
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 3 cut(s) 293, 978, 1099
GlaI GCGC 4 cut(s) 9, 106, 281, 1191
GluI GCNGC 3 cut(s) 293, 978, 1099
GsaI CCCAGC 1 cut(s) 816
HaeII RGCGCY 2 cut(s) 11, 1193
HaeIII GGCC 4 cut(s) 49, 217, 633, 750
HapII CCGG 5 cut(s) 59, 108, 220, 796, 1241
HhaI GCGC 4 cut(s) 10, 107, 282, 1192
Hin6I GCGC 4 cut(s) 8, 105, 280, 1190
HinP1I GCGC 4 cut(s) 8, 105, 280, 1190
HincII GTYRAC 2 cut(s) 88, 1197
HindII GTYRAC 2 cut(s) 88, 1197
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 4 cut(s) 89, 182, 851, 1505
HpaII CCGG 5 cut(s) 59, 108, 220, 796, 1241
HphI GGTGA 2 cut(s) 438, 871
Hpy166II GTNNAC 5 cut(s) 82, 88, 442, 1181, 1197
Hpy188I TCNGA 7 cut(s) 125, 181, 206, 571, 652, 1156, 1510
Hpy188III TCNNGA 4 cut(s) 846, 956, 1313, 1457
Hpy8I GTNNAC 5 cut(s) 82, 88, 442, 1181, 1197
Hpy99I CGWCG 4 cut(s) 55, 89, 323, 612
HpyAV CCTTC 3 cut(s) 519, 589, 1083
HpyCH4III ACNGT 4 cut(s) 629, 712, 1039, 1502
HpyCH4IV ACGT 4 cut(s) 84, 155, 196, 745
HpyF10VI GCNNNNNNNGC 6 cut(s) 104, 117, 714, 764, 787, 1104
HpyF3I CTNAG 4 cut(s) 116, 390, 728, 855
HpySE526I ACGT 4 cut(s) 84, 155, 196, 745
HspAI GCGC 4 cut(s) 8, 105, 280, 1190
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 2 cut(s) 463, 1465
Kzo9I GATC 3 cut(s) 463, 1213, 1465
LmnI GCTCC 1 cut(s) 671
Lsp1109I GCAGC 3 cut(s) 279, 964, 1110
LweI GCATC 3 cut(s) 78, 1033, 1397
MaeII ACGT 4 cut(s) 84, 155, 196, 745
MaeIII GTNAC 6 cut(s) 54, 451, 556, 623, 1228, 1372
MalI GATC 3 cut(s) 465, 1215, 1467
MboI GATC 3 cut(s) 463, 1213, 1465
MboII GAAGA 7 cut(s) 15, 376, 713, 891, 1144, 1409, 1472
MfeI CAATTG 2 cut(s) 962, 999
MflI RGATCY 1 cut(s) 1213
MhlI GDGCHC 4 cut(s) 398, 819, 937, 1348
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 8 cut(s) 296, 337, 477, 656, 962, 999, 1291, 1368
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 2 cut(s) 83, 176
MmeI TCCRAC 2 cut(s) 159, 204
Mox20I TGGCCA 1 cut(s) 750
MroNI GCCGGC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 750
MseI TTAA 6 cut(s) 431, 458, 761, 948, 1257, 1452
MslI CAYNNNNRTG 3 cut(s) 77, 143, 1314
Msp20I TGGCCA 1 cut(s) 750
MspI CCGG 5 cut(s) 59, 108, 220, 796, 1241
MspR9I CCNGG 3 cut(s) 220, 1139, 1241
MunI CAATTG 2 cut(s) 962, 999
MvaI CCWGG 1 cut(s) 1139
MwoI GCNNNNNNNGC 6 cut(s) 104, 117, 714, 764, 787, 1104
NaeI GCCGGC 1 cut(s) 109
NciI CCSGG 2 cut(s) 220, 1241
NdeI CATATG 1 cut(s) 1519
NdeII GATC 3 cut(s) 463, 1213, 1465
NgoMIV GCCGGC 1 cut(s) 107
NlaIV GGNNCC 6 cut(s) 218, 934, 985, 1144, 1215, 1487
NmuCI GTSAC 2 cut(s) 451, 623
NspI RCATGY 2 cut(s) 142, 758
OliI CACNNNNGTG 1 cut(s) 77
PaeI GCATGC 1 cut(s) 758
PagI TCATGA 1 cut(s) 955
PciI ACATGT 1 cut(s) 138
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 851, 1505
PkrI GCNGC 3 cut(s) 294, 979, 1100
PleI GAGTC 2 cut(s) 83, 176
PpsI GAGTC 2 cut(s) 83, 176
Ppu21I YACGTR 1 cut(s) 156
PscI ACATGT 1 cut(s) 138
Psp1406I AACGTT 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 1137
PspFI CCCAGC 1 cut(s) 812
PspGI CCWGG 1 cut(s) 1137
PspN4I GGNNCC 6 cut(s) 218, 934, 985, 1144, 1215, 1487
PspPI GGNCC 2 cut(s) 216, 632
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 1 cut(s) 1213
RsaI GTAC 2 cut(s) 158, 1055
RsaNI GTAC 2 cut(s) 157, 1054
RseI CAYNNNNRTG 3 cut(s) 77, 143, 1314
SalI GTCGAC 1 cut(s) 86
SaqAI TTAA 6 cut(s) 431, 458, 761, 948, 1257, 1452
SatI GCNGC 3 cut(s) 293, 978, 1099
Sau3AI GATC 3 cut(s) 463, 1213, 1465
Sau96I GGNCC 2 cut(s) 216, 632
SchI GAGTC 2 cut(s) 83, 176
ScrFI CCNGG 3 cut(s) 220, 1139, 1241
SduI GDGCHC 4 cut(s) 398, 819, 937, 1348
SfaNI GCATC 3 cut(s) 78, 1033, 1397
SfcI CTRYAG 2 cut(s) 939, 1035
SmiMI CAYNNNNRTG 3 cut(s) 77, 143, 1314
SphI GCATGC 1 cut(s) 758
Sse9I AATT 8 cut(s) 296, 337, 477, 656, 962, 999, 1291, 1368
SsiI CCGC 2 cut(s) 234, 1166
StyD4I CCNGG 3 cut(s) 218, 1137, 1239
StyI CCWWGG 1 cut(s) 1296
TaaI ACNGT 4 cut(s) 629, 712, 1039, 1502
TaiI ACGT 4 cut(s) 87, 158, 199, 748
TaqI TCGA 4 cut(s) 87, 351, 523, 1366
TasI AATT 8 cut(s) 296, 337, 477, 656, 962, 999, 1291, 1368
TatI WGTACW 1 cut(s) 1053
TfiI GAWTC 2 cut(s) 851, 1505
Tru1I TTAA 6 cut(s) 431, 458, 761, 948, 1257, 1452
Tru9I TTAA 6 cut(s) 431, 458, 761, 948, 1257, 1452
TscAI CASTG 6 cut(s) 79, 507, 634, 742, 1282, 1507
TseFI GTSAC 2 cut(s) 451, 623
TseI GCWGC 3 cut(s) 292, 977, 1098
Tsp45I GTSAC 2 cut(s) 451, 623
TspDTI ATGAA 6 cut(s) 522, 891, 972, 1332, 1410, 1506
TspGWI ACGGA 1 cut(s) 829
TspRI CASTG 6 cut(s) 79, 507, 634, 742, 1282, 1507
XagI CCTNNNNNAGG 1 cut(s) 164
XceI RCATGY 2 cut(s) 142, 758
XmiI GTMKAC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.