MD09G1089400.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007523
Physical Location & Seq
Reverse (-)
6557188 .. 6557922
735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1089400.v1.1.491

Sequence Viewer

Length: 735 bp
ATGCACTCTGGTCCTAACCAACATCCACCGCCACACATGATGCATCAAGATCCAGGTTATCCAGGTCCCAATGGCCGCAATCCTCCTCGCTTTAATGGCCAGTACCGCCCTAATGGGCGCCGCAAAAACAGTTGTCTGAGATGCTACTGTTGGTGTTGTTGTTGCATCTTCGTAACCCTAGTGGTAATCATCGCTGCCGTGGCCATCAGCCTAGTAATTATCAATCCCCGTAAGCCCAAATACAGTATTAGTGACTTCTCTGTCAAGGCATTCAACCTCACCCCTGATTACAGCCTCCATGCGCAATTTGTGGTCACGGTAAAGGCCGAGAATCCCAACAAAAGAATATCCATTGTATATGGGAAGGGCAGCACCGTGCAGCTCAAGTATTCCGGGATGAAACTTTGTACCGGGGTGGTCCCACATTTTCGTCAACCGACGAGAAATACCACCTTGATGGACGTGGACTTGAGAGGAGACTTGAAAGGGAGTGACTTTAAGGGCCCTATCGGGGAGTCACTTATGCAGAAAATCAAGAGTGGCAGGATCCCTGTGGCTGTTTCGGTTAGGGTACCGGTCAATGTAGGACTCGGAGCGTACAATGTATTGCCGGATCCAATCGGGATTTATGTTGACGCCTCCATGGTGGTCAACAATTTGTCCCAGCCAGATAAGAAAGTACAGATTTCAGACCCTAAATATCACTTTAATTTTGGTTATAAGTTCAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

245

Amino Acids

27.15

Weight (kDa)

9.77

Isoelectric Point (pI)

19.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 106 - 193 1.1e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AasI GACNNNNNNGTC 1 cut(s) 260
Acc16I TGCGCA 1 cut(s) 303
Acc65I GGTACC 1 cut(s) 571
AccB1I GGYRCC 2 cut(s) 117, 571
AciI CCGC 4 cut(s) 29, 76, 106, 121
AclWI GGATC 5 cut(s) 44, 541, 554, 608, 621
AcoI YGGCCR 3 cut(s) 73, 97, 201
AcyI GRCGYC 2 cut(s) 118, 636
AfaI GTAC 5 cut(s) 104, 409, 573, 599, 681
AfiI CCNNNNNNNGG 1 cut(s) 511
AgeI ACCGGT 1 cut(s) 574
AgsI TTSAA 3 cut(s) 274, 484, 727
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 2 cut(s) 52, 61
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 5 cut(s) 44, 541, 554, 608, 621
AoxI GGCC 5 cut(s) 73, 97, 201, 324, 502
ApaI GGGCCC 1 cut(s) 506
ApeKI GCWGC 3 cut(s) 194, 369, 379
AsiGI ACCGGT 1 cut(s) 574
Asp718I GGTACC 1 cut(s) 571
AspLEI GCGC 2 cut(s) 120, 304
AspS9I GGNCC 5 cut(s) 11, 65, 418, 502, 503
AsuC2I CCSGG 2 cut(s) 394, 412
AsuHPI GGTGA 1 cut(s) 271
AvaII GGWCC 3 cut(s) 11, 65, 418
BaeGI GKGCMC 1 cut(s) 506
BalI TGGCCA 2 cut(s) 99, 203
BamHI GGATCC 2 cut(s) 546, 613
BanI GGYRCC 2 cut(s) 117, 571
BanII GRGCYC 1 cut(s) 506
BbvI GCAGC 3 cut(s) 181, 381, 391
BccI CCATC 2 cut(s) 212, 451
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 2 cut(s) 54, 63
BcnI CCSGG 2 cut(s) 394, 412
BcoDI GTCTC 1 cut(s) 471
BfaI CTAG 2 cut(s) 179, 212
BfoI RGCGCY 1 cut(s) 121
BisI GCNGC 5 cut(s) 76, 121, 195, 370, 380
BlsI GCNGC 5 cut(s) 77, 122, 196, 371, 381
Bme1390I CCNGG 4 cut(s) 54, 63, 394, 412
Bme18I GGWCC 3 cut(s) 11, 65, 418
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 5 cut(s) 11, 65, 418, 502, 503
BmiI GGNNCC 7 cut(s) 67, 119, 420, 504, 548, 573, 615
BmrFI CCNGG 4 cut(s) 54, 63, 394, 412
BmsI GCATC 4 cut(s) 30, 52, 131, 174
BpuEI CTTGAG 2 cut(s) 368, 490
BpuMI CCSGG 2 cut(s) 394, 412
BsaHI GRCGYC 2 cut(s) 118, 636
BsaJI CCNNGG 3 cut(s) 198, 411, 642
BsaWI WCCGGW 1 cut(s) 574
Bsc4I CCNNNNNNNGG 1 cut(s) 511
Bse118I RCCGGY 1 cut(s) 574
Bse1I ACTGG 1 cut(s) 100
BseBI CCWGG 2 cut(s) 54, 63
BseDI CCNNGG 3 cut(s) 198, 411, 642
BseGI GGATG 2 cut(s) 22, 402
BseLI CCNNNNNNNGG 1 cut(s) 511
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 2 cut(s) 75, 489
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 3 cut(s) 181, 381, 391
BseYI CCCAGC 1 cut(s) 663
BsgI GTGCAG 1 cut(s) 398
BshFI GGCC 5 cut(s) 75, 99, 203, 326, 504
BshNI GGYRCC 2 cut(s) 117, 571
BshTI ACCGGT 1 cut(s) 574
BsiSI CCGG 4 cut(s) 393, 411, 575, 611
BslFI GGGAC 3 cut(s) 51, 404, 646
BslI CCNNNNNNNGG 1 cut(s) 511
BsmAI GTCTC 1 cut(s) 471
BsmFI GGGAC 3 cut(s) 51, 404, 646
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 5 cut(s) 75, 99, 203, 326, 504
Bsp120I GGGCCC 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 3 cut(s) 49, 546, 613
Bsp19I CCATGG 1 cut(s) 642
BspACI CCGC 4 cut(s) 29, 76, 106, 121
BspANI GGCC 5 cut(s) 75, 99, 203, 326, 504
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 7 cut(s) 67, 119, 420, 504, 548, 573, 615
BspPI GGATC 5 cut(s) 44, 541, 554, 608, 621
BspT107I GGYRCC 2 cut(s) 117, 571
BsrFI RCCGGY 1 cut(s) 574
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 574
BssECI CCNNGG 3 cut(s) 198, 411, 642
BssMI GATC 3 cut(s) 49, 546, 613
BssNI GRCGYC 2 cut(s) 118, 636
BssT1I CCWWGG 1 cut(s) 642
Bst2UI CCWGG 2 cut(s) 54, 63
Bst4CI ACNGT 5 cut(s) 131, 149, 245, 319, 376
BstACI GRCGYC 2 cut(s) 118, 636
BstDEI CTNAG 1 cut(s) 137
BstDSI CCRYGG 2 cut(s) 198, 642
BstF5I GGATG 2 cut(s) 22, 402
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 120, 304
BstKTI GATC 3 cut(s) 52, 549, 616
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 3 cut(s) 49, 546, 613
BstMWI GCNNNNNNNGC 3 cut(s) 96, 105, 200
BstNI CCWGG 2 cut(s) 54, 63
BstSCI CCNGG 4 cut(s) 52, 61, 392, 410
BstSLI GKGCMC 1 cut(s) 506
BstV1I GCAGC 3 cut(s) 181, 381, 391
BstX2I RGATCY 3 cut(s) 49, 546, 613
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 3 cut(s) 49, 546, 613
BsuRI GGCC 5 cut(s) 75, 99, 203, 326, 504
BtgI CCRYGG 2 cut(s) 198, 642
BtgZI GCGATG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 22, 402
CfoI GCGC 2 cut(s) 120, 304
Cfr10I RCCGGY 1 cut(s) 574
Cfr13I GGNCC 5 cut(s) 11, 65, 418, 502, 503
CseI GACGC 1 cut(s) 644
Csp6I GTAC 5 cut(s) 103, 408, 572, 598, 680
CspAI ACCGGT 1 cut(s) 574
CviAII CATG 3 cut(s) 37, 299, 643
CviQI GTAC 5 cut(s) 103, 408, 572, 598, 680
DdeI CTNAG 1 cut(s) 137
DinI GGCGCC 1 cut(s) 119
DpnI GATC 3 cut(s) 51, 548, 615
DpnII GATC 3 cut(s) 49, 546, 613
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 3 cut(s) 73, 97, 201
Eco130I CCWWGG 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 506
Eco47I GGWCC 3 cut(s) 11, 65, 418
EcoO109I RGGNCCY 3 cut(s) 65, 502, 503
EcoRII CCWGG 2 cut(s) 52, 61
EcoT14I CCWWGG 1 cut(s) 642
EcoT22I ATGCAT 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 506
EgeI GGCGCC 1 cut(s) 119
EheI GGCGCC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 642
FaeI CATG 3 cut(s) 40, 302, 646
FaiI YATR 8 cut(s) 38, 300, 358, 360, 524, 630, 644, 720
FaqI GGGAC 3 cut(s) 51, 404, 646
FatI CATG 3 cut(s) 36, 298, 642
Fnu4HI GCNGC 5 cut(s) 76, 121, 195, 370, 380
FokI GGATG 2 cut(s) 9, 409
FriOI GRGCYC 1 cut(s) 506
Fsp4HI GCNGC 5 cut(s) 76, 121, 195, 370, 380
FspBI CTAG 2 cut(s) 179, 212
FspI TGCGCA 1 cut(s) 303
GlaI GCGC 2 cut(s) 119, 303
GluI GCNGC 5 cut(s) 76, 121, 195, 370, 380
GsaI CCCAGC 1 cut(s) 667
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 5 cut(s) 75, 99, 203, 326, 504
HapII CCGG 4 cut(s) 393, 411, 575, 611
HgaI GACGC 1 cut(s) 644
HhaI GCGC 2 cut(s) 120, 304
Hin1I GRCGYC 2 cut(s) 118, 636
Hin1II CATG 3 cut(s) 40, 302, 646
Hin6I GCGC 2 cut(s) 118, 302
HinP1I GCGC 2 cut(s) 118, 302
HincII GTYRAC 3 cut(s) 434, 634, 652
HindII GTYRAC 3 cut(s) 434, 634, 652
HinfI GANTC 3 cut(s) 331, 515, 588
HpaII CCGG 4 cut(s) 393, 411, 575, 611
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 4 cut(s) 434, 466, 634, 652
Hpy188I TCNGA 3 cut(s) 138, 593, 691
Hpy188III TCNNGA 4 cut(s) 47, 535, 622, 727
Hpy8I GTNNAC 4 cut(s) 434, 466, 634, 652
Hpy99I CGWCG 1 cut(s) 442
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 5 cut(s) 131, 149, 245, 319, 376
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 5 cut(s) 4, 43, 165, 379, 526
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 105, 200
HpyF3I CTNAG 1 cut(s) 137
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 2 cut(s) 118, 636
Hsp92II CATG 3 cut(s) 40, 302, 646
HspAI GCGC 2 cut(s) 118, 302
KasI GGCGCC 1 cut(s) 117
KpnI GGTACC 1 cut(s) 575
Kzo9I GATC 3 cut(s) 49, 546, 613
LmnI GCTCC 1 cut(s) 593
Lsp1109I GCAGC 3 cut(s) 181, 381, 391
LweI GCATC 4 cut(s) 30, 52, 131, 174
MaeI CTAG 2 cut(s) 179, 212
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 5 cut(s) 172, 251, 313, 491, 516
MalI GATC 3 cut(s) 51, 548, 615
MboI GATC 3 cut(s) 49, 546, 613
MboII GAAGA 1 cut(s) 160
MflI RGATCY 3 cut(s) 49, 546, 613
MhlI GDGCHC 1 cut(s) 506
MlsI TGGCCA 2 cut(s) 99, 203
MluCI AATT 4 cut(s) 216, 305, 655, 709
MluNI TGGCCA 2 cut(s) 99, 203
Mly113I GGCGCC 1 cut(s) 118
MlyI GAGTC 2 cut(s) 524, 582
MnlI CCTC 6 cut(s) 93, 96, 287, 305, 467, 649
Mox20I TGGCCA 2 cut(s) 99, 203
Mph1103I ATGCAT 1 cut(s) 45
MscI TGGCCA 2 cut(s) 99, 203
MseI TTAA 3 cut(s) 93, 498, 708
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 2 cut(s) 99, 203
MspI CCGG 4 cut(s) 393, 411, 575, 611
MspR9I CCNGG 4 cut(s) 54, 63, 394, 412
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 2 cut(s) 54, 63
MwoI GCNNNNNNNGC 3 cut(s) 96, 105, 200
NarI GGCGCC 1 cut(s) 118
NciI CCSGG 2 cut(s) 394, 412
NcoI CCATGG 1 cut(s) 642
NdeII GATC 3 cut(s) 49, 546, 613
NlaIII CATG 3 cut(s) 40, 302, 646
NlaIV GGNNCC 7 cut(s) 67, 119, 420, 504, 548, 573, 615
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 4 cut(s) 251, 313, 491, 516
NsbI TGCGCA 1 cut(s) 303
NsiI ATGCAT 1 cut(s) 45
PctI GAATGC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 392
PinAI ACCGGT 1 cut(s) 574
PkrI GCNGC 5 cut(s) 77, 122, 196, 371, 381
PleI GAGTC 2 cut(s) 523, 582
PluTI GGCGCC 1 cut(s) 121
PpsI GAGTC 2 cut(s) 523, 582
PpuMI RGGWCCY 1 cut(s) 65
PsiI TTATAA 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 52, 61
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 2 cut(s) 52, 61
PspN4I GGNNCC 7 cut(s) 67, 119, 420, 504, 548, 573, 615
PspOMI GGGCCC 1 cut(s) 502
PspPI GGNCC 5 cut(s) 11, 65, 418, 502, 503
PspPPI RGGWCCY 1 cut(s) 65
PsuI RGATCY 3 cut(s) 49, 546, 613
RsaI GTAC 5 cut(s) 104, 409, 573, 599, 681
RsaNI GTAC 5 cut(s) 103, 408, 572, 598, 680
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 3 cut(s) 93, 498, 708
SatI GCNGC 5 cut(s) 76, 121, 195, 370, 380
Sau3AI GATC 3 cut(s) 49, 546, 613
Sau96I GGNCC 5 cut(s) 11, 65, 418, 502, 503
SchI GAGTC 2 cut(s) 524, 582
ScrFI CCNGG 4 cut(s) 54, 63, 394, 412
SduI GDGCHC 1 cut(s) 506
SetI ASST 6 cut(s) 58, 67, 279, 384, 455, 465
SfaNI GCATC 4 cut(s) 30, 52, 131, 174
SfoI GGCGCC 1 cut(s) 119
SinI GGWCC 3 cut(s) 11, 65, 418
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 383, 469
SmoI CTYRAG 2 cut(s) 383, 469
Sse9I AATT 4 cut(s) 216, 305, 655, 709
SsiI CCGC 4 cut(s) 29, 76, 106, 121
SspDI GGCGCC 1 cut(s) 117
SspMI CTAG 2 cut(s) 179, 212
StyD4I CCNGG 4 cut(s) 52, 61, 392, 410
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 5 cut(s) 131, 149, 245, 319, 376
TaiI ACGT 1 cut(s) 465
TasI AATT 4 cut(s) 216, 305, 655, 709
TatI WGTACW 1 cut(s) 679
TauI GCSGC 2 cut(s) 78, 123
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 3 cut(s) 93, 498, 708
Tru9I TTAA 3 cut(s) 93, 498, 708
TseFI GTSAC 4 cut(s) 251, 313, 491, 516
TseI GCWGC 3 cut(s) 194, 369, 379
Tsp45I GTSAC 4 cut(s) 251, 313, 491, 516
TspDTI ATGAA 1 cut(s) 413
VpaK11BI GGWCC 3 cut(s) 11, 65, 418
XspI CTAG 2 cut(s) 179, 212
Zsp2I ATGCAT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.