MD09G1134600.v1.1
MYB Family

Myb family transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012464
Physical Location & Seq
Forward (+)
10349711 .. 10351965
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1134600.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGTTGGTTTTGGCTGTGGTGGTGATGATGAACAGGATTTTCAAGGATCTTCACCGACTCCTACCGTCACGCATAGCAGTGATAGCGTTGTTGTGGAGGGTGAGGAGGCGGCAGCAGTGGCGGAGGAGGAGGAGGAGGTTGATCGCGAAACGTGTGATCTTCAGCTGCCTCAAGACCGAGTTCTGGCTGCGAAGCTCAGACCCCGCCTTCGATGGACTCCTGAGCTTCATGCCTGCTTTGTTGATGCTGTCAATCAGCTTGGAGGCCTTCATAAGGCAACTCCGAAGAAAATCCAGGAGGCAATGGGTGTCCAGGGGATAACTCTTTTCCACTTGAAGAGCCACCTCCAGAAATACAGACTTGGAAGGCACGGGGTGAAGGAATGGAGGGAGGGGACTCAAACCTTTTCACAGGACTCGGAAGAAGGCCGAAGCACCAGTAGTACTTCCTCTCCCTCGCCCAAGCTATCCCGGAAAAAGCACTCTTATAAACTAGTGAAGGAAGCTCTAGATGAAGAAAGAGATAGTATGCAAGTTGAGGTTGGGAGGCGATTGCATATCCGTCAGGATGCTGAGCGGAGATTCATGAATTTCGCGATGGAGAATGCATGTAAGAAGCTTGCTGATCAGTTTATTGGTGCTGCGACAGCCGCAGCCATACTTAACGGCATTGCAGGTGTAGGAACTTTGGAACTATACCCTGCATATCAGATGACAGAGGGAACGGGTATGCAGCCTAGTCTTGAAGGCCACACGACCTCTCACGGGCATTCGGAGAATTCATCTGTCGAAGATTTCCATACTCGTGCCGAAGATGACGAGAAAACGGAGGAGAGCTTGGACAATGACCCTGCAGAAGCTTACCTGATCAACCCGGATCCAGATATGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

298

Amino Acids

32.85

Weight (kDa)

4.97

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 69 - 120 1.3e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06020 AT3G12730 AT3G12730
fragaria_vesca FvH4_6g40170 FvH4_6g40170 FvH4_6g40170
malus_domestica MD09G1134600.v1.1 MD17G1123500.v1.1
prunus_persica Prupe.3G192100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0155181
rosa_laevigata RLG00000020824
rosa_multiflora Rmu_sc0004976.1_g000035
rosa_roxburghii Rroxscaffold_2G00093890
rosa_rugosa Rorug02G0445800
rosa_samantha Rh2AG512000 Rh2BG522800 Rh2CG497000 Rh2DG532500
rosa_wichuraiana Rw2G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AarI CACCTGC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 577
AccII CGCG 2 cut(s) 147, 596
AciI CCGC 5 cut(s) 110, 122, 205, 577, 651
AclWI GGATC 3 cut(s) 55, 872, 885
AcsI RAATTY 2 cut(s) 589, 778
AcuI CTGAAG 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 1 cut(s) 445
AfiI CCNNNNNNNGG 3 cut(s) 184, 274, 765
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 3 cut(s) 44, 337, 746
AhlI ACTAGT 1 cut(s) 493
AjnI CCWGG 2 cut(s) 294, 312
AluBI AGCT 9 cut(s) 166, 196, 226, 259, 466, 506, 619, 837, 860
AluI AGCT 9 cut(s) 166, 196, 226, 259, 466, 506, 619, 837, 860
AlwI GGATC 3 cut(s) 55, 872, 885
AoxI GGCC 3 cut(s) 265, 427, 748
ApeKI GCWGC 6 cut(s) 113, 166, 188, 641, 653, 733
ApoI RAATTY 2 cut(s) 589, 778
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AsuC2I CCSGG 2 cut(s) 472, 875
AsuHPI GGTGA 4 cut(s) 35, 45, 113, 388
BamHI GGATCC 1 cut(s) 877
BauI CACGAG 1 cut(s) 804
BbvI GCAGC 6 cut(s) 125, 153, 175, 628, 665, 745
BccI CCATC 2 cut(s) 207, 592
BceAI ACGGC 1 cut(s) 682
BciT130I CCWGG 2 cut(s) 296, 314
BclI TGATCA 2 cut(s) 625, 867
BcnI CCSGG 2 cut(s) 472, 875
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 3 cut(s) 494, 509, 738
BfmI CTRYAG 1 cut(s) 852
BfuAI ACCTGC 1 cut(s) 665
BisI GCNGC 8 cut(s) 111, 114, 167, 189, 642, 651, 654, 734
BlpI GCTNAGC 1 cut(s) 573
BlsI GCNGC 8 cut(s) 112, 115, 168, 190, 643, 652, 655, 735
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 4 cut(s) 296, 314, 472, 875
BmiI GGNNCC 1 cut(s) 879
BmrFI CCNGG 4 cut(s) 296, 314, 472, 875
BmsI GCATC 2 cut(s) 235, 559
BpmI CTGGAG 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 156
BpuMI CCSGG 2 cut(s) 472, 875
BsaJI CCNNGG 1 cut(s) 313
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 274, 765
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 2 cut(s) 309, 669
BseBI CCWGG 2 cut(s) 296, 314
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 1 cut(s) 574
BseLI CCNNNNNNNGG 3 cut(s) 184, 274, 765
BseMI GCAATG 2 cut(s) 309, 669
BseMII CTCAG 3 cut(s) 211, 213, 564
BseNI ACTGG 1 cut(s) 438
BseRI GAGGAG 6 cut(s) 119, 140, 143, 146, 149, 845
BseXI GCAGC 6 cut(s) 125, 153, 175, 628, 665, 745
Bsh1236I CGCG 2 cut(s) 147, 596
BshFI GGCC 3 cut(s) 267, 429, 750
BsiSI CCGG 2 cut(s) 472, 875
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 3 cut(s) 184, 274, 765
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 2 cut(s) 610, 769
BsnI GGCC 3 cut(s) 267, 429, 750
Bsp143I GATC 6 cut(s) 47, 142, 157, 625, 867, 877
Bsp1720I GCTNAGC 1 cut(s) 573
Bsp68I TCGCGA 2 cut(s) 147, 596
BspACI CCGC 5 cut(s) 110, 122, 205, 577, 651
BspANI GGCC 3 cut(s) 267, 429, 750
BspCNI CTCAG 3 cut(s) 210, 214, 565
BspFNI CGCG 2 cut(s) 147, 596
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 1 cut(s) 879
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 1 cut(s) 665
BspPI GGATC 3 cut(s) 55, 872, 885
BspQI GCTCTTC 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 577
BsrDI GCAATG 2 cut(s) 309, 669
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 6 cut(s) 47, 142, 157, 625, 867, 877
BssSI CACGAG 1 cut(s) 804
Bst2BI CACGAG 1 cut(s) 804
Bst2UI CCWGG 2 cut(s) 296, 314
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 235, 621
BstDEI CTNAG 3 cut(s) 197, 222, 573
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 574
BstFNI CGCG 2 cut(s) 147, 596
BstKTI GATC 6 cut(s) 50, 145, 160, 628, 870, 880
BstMBI GATC 6 cut(s) 47, 142, 157, 625, 867, 877
BstMWI GCNNNNNNNGC 4 cut(s) 84, 119, 647, 650
BstNI CCWGG 2 cut(s) 296, 314
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 4 cut(s) 294, 312, 470, 873
BstSFI CTRYAG 1 cut(s) 852
BstUI CGCG 2 cut(s) 147, 596
BstV1I GCAGC 6 cut(s) 125, 153, 175, 628, 665, 745
BstX2I RGATCY 2 cut(s) 47, 877
BstYI RGATCY 2 cut(s) 47, 877
BsuRI GGCC 3 cut(s) 267, 429, 750
BtgZI GCGATG 1 cut(s) 611
BtsCI GGATG 1 cut(s) 574
BtsI GCAGTG 2 cut(s) 85, 123
BtsIMutI CAGTG 2 cut(s) 85, 123
BtuMI TCGCGA 2 cut(s) 147, 596
BveI ACCTGC 1 cut(s) 665
Cac8I GCNNGC 2 cut(s) 235, 621
CciI TCATGA 1 cut(s) 585
Csp6I GTAC 1 cut(s) 444
CviAII CATG 3 cut(s) 230, 586, 609
CviQI GTAC 1 cut(s) 444
DdeI CTNAG 3 cut(s) 197, 222, 573
DpnI GATC 6 cut(s) 49, 144, 159, 627, 869, 879
DpnII GATC 6 cut(s) 47, 142, 157, 625, 867, 877
DraIII CACNNNGTG 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
EciI GGCGGA 1 cut(s) 137
Eco147I AGGCCT 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 146
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 294, 312
EcoT22I ATGCAT 1 cut(s) 610
FaeI CATG 3 cut(s) 233, 589, 612
FaqI GGGAC 1 cut(s) 409
FatI CATG 3 cut(s) 229, 585, 608
FauI CCCGC 1 cut(s) 212
FbaI TGATCA 2 cut(s) 625, 867
Fnu4HI GCNGC 8 cut(s) 111, 114, 167, 189, 642, 651, 654, 734
FokI GGATG 1 cut(s) 581
Fsp4HI GCNGC 8 cut(s) 111, 114, 167, 189, 642, 651, 654, 734
FspBI CTAG 3 cut(s) 494, 509, 738
GluI GCNGC 8 cut(s) 111, 114, 167, 189, 642, 651, 654, 734
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 3 cut(s) 267, 429, 750
HapII CCGG 2 cut(s) 472, 875
Hin1II CATG 3 cut(s) 233, 589, 612
HindIII AAGCTT 2 cut(s) 617, 858
HinfI GANTC 5 cut(s) 58, 217, 397, 416, 582
HpaII CCGG 2 cut(s) 472, 875
HphI GGTGA 4 cut(s) 35, 45, 113, 388
Hpy188I TCNGA 5 cut(s) 200, 285, 421, 711, 775
HpyAV CCTTC 7 cut(s) 218, 278, 360, 373, 419, 493, 740
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 7 cut(s) 532, 556, 608, 674, 704, 733, 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 119, 647, 650
HpyF3I CTNAG 3 cut(s) 197, 222, 573
HpySE526I ACGT 1 cut(s) 152
Hsp92II CATG 3 cut(s) 233, 589, 612
Ksp22I TGATCA 2 cut(s) 625, 867
Kzo9I GATC 6 cut(s) 47, 142, 157, 625, 867, 877
LguI GCTCTTC 1 cut(s) 332
Lsp1109I GCAGC 6 cut(s) 125, 153, 175, 628, 665, 745
LweI GCATC 2 cut(s) 235, 559
MaeI CTAG 3 cut(s) 494, 509, 738
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 67
MalI GATC 6 cut(s) 49, 144, 159, 627, 869, 879
MbiI CCGCTC 1 cut(s) 577
MboI GATC 6 cut(s) 47, 142, 157, 625, 867, 877
MboII GAAGA 8 cut(s) 42, 152, 298, 349, 434, 527, 803, 824
MflI RGATCY 2 cut(s) 47, 877
MluCI AATT 2 cut(s) 589, 778
MlyI GAGTC 4 cut(s) 52, 211, 391, 410
Mph1103I ATGCAT 1 cut(s) 610
MseI TTAA 1 cut(s) 663
MslI CAYNNNNRTG 2 cut(s) 78, 804
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 2 cut(s) 472, 875
MspR9I CCNGG 4 cut(s) 296, 314, 472, 875
Mva1269I GAATGC 2 cut(s) 610, 769
MvaI CCWGG 2 cut(s) 296, 314
MvnI CGCG 2 cut(s) 147, 596
MwoI GCNNNNNNNGC 4 cut(s) 84, 119, 647, 650
NciI CCSGG 2 cut(s) 472, 875
NdeII GATC 6 cut(s) 47, 142, 157, 625, 867, 877
NlaIII CATG 3 cut(s) 233, 589, 612
NlaIV GGNNCC 1 cut(s) 879
NmuCI GTSAC 1 cut(s) 67
NruI TCGCGA 2 cut(s) 147, 596
NsiI ATGCAT 1 cut(s) 610
NspI RCATGY 1 cut(s) 612
PagI TCATGA 1 cut(s) 585
PaqCI CACCTGC 1 cut(s) 665
PceI AGGCCT 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 332
PctI GAATGC 2 cut(s) 610, 769
PfeI GAWTC 1 cut(s) 582
PfoI TCCNGGA 2 cut(s) 294, 470
PkrI GCNGC 8 cut(s) 112, 115, 168, 190, 643, 652, 655, 735
PleI GAGTC 4 cut(s) 52, 211, 391, 410
PpsI GAGTC 4 cut(s) 52, 211, 391, 410
PsiI TTATAA 1 cut(s) 489
Psp6I CCWGG 2 cut(s) 294, 312
PspGI CCWGG 2 cut(s) 294, 312
PspN4I GGNNCC 1 cut(s) 879
PstI CTGCAG 1 cut(s) 856
PsuI RGATCY 2 cut(s) 47, 877
PvuII CAGCTG 1 cut(s) 166
RruI TCGCGA 2 cut(s) 147, 596
RsaI GTAC 1 cut(s) 445
RsaNI GTAC 1 cut(s) 444
RseI CAYNNNNRTG 2 cut(s) 78, 804
SapI GCTCTTC 1 cut(s) 332
SaqAI TTAA 1 cut(s) 663
SatI GCNGC 8 cut(s) 111, 114, 167, 189, 642, 651, 654, 734
Sau3AI GATC 6 cut(s) 47, 142, 157, 625, 867, 877
ScaI AGTACT 1 cut(s) 445
SchI GAGTC 4 cut(s) 52, 211, 391, 410
ScrFI CCNGG 4 cut(s) 296, 314, 472, 875
SfaNI GCATC 2 cut(s) 235, 559
SfcI CTRYAG 1 cut(s) 852
SmiMI CAYNNNNRTG 2 cut(s) 78, 804
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 2 cut(s) 589, 778
SseBI AGGCCT 1 cut(s) 267
SsiI CCGC 5 cut(s) 110, 122, 205, 577, 651
SspMI CTAG 3 cut(s) 494, 509, 738
StuI AGGCCT 1 cut(s) 267
StyD4I CCNGG 4 cut(s) 294, 312, 470, 873
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 155
TaqI TCGA 2 cut(s) 211, 789
TaqII GACCGA 1 cut(s) 192
TasI AATT 2 cut(s) 589, 778
TatI WGTACW 1 cut(s) 443
TauI GCSGC 2 cut(s) 113, 653
TfiI GAWTC 1 cut(s) 582
Tru1I TTAA 1 cut(s) 663
Tru9I TTAA 1 cut(s) 663
TscAI CASTG 2 cut(s) 85, 123
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 6 cut(s) 113, 166, 188, 641, 653, 733
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 7 cut(s) 45, 218, 260, 528, 574, 602, 771
TspGWI ACGGA 2 cut(s) 551, 842
TspRI CASTG 2 cut(s) 85, 123
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 2 cut(s) 589, 778
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 1 cut(s) 612
XspI CTAG 3 cut(s) 494, 509, 738
ZrmI AGTACT 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.