Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008313

Physical Location & Seq

Genomic Coordinates Reverse (-)

13050757 .. 13055911

Gene Structure 5155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1161600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1287 bp

ATGGTTAGGCCCTCCGCGAAGCACACATCGGCTCGGCTGCTGTTGATATGCGTCGGTCTGCTGGGGATTGCTCTGATTGCCGATTTCCTGTGGGCTTCCTCTCCTCGCTTTATCCGTTCTTATAACCACATTGCCGACAATTGGGCTCCTCCTGTTGATATCTCAAAACCCACTAGCATCCCCTCCCCTAAGAAAACTAGGCATGAAATCCCCACCAAGGGAAGCGTCGATTACAAAAATGATACTGCTCCTGGAAGAGTTTTATCAGCAACCTTCGCTGATTTGCCTGGACCAGAATTAAAATGGGAAGGTATGGCTGAGGCACCCGTACCTCGTTTGGATGGGGCTGCTATACAGATAAAGAATCTTCTCTTCGTGTTTGCTGGATATGGTACCATTGATTATGTACATTCGCATGTTGATATCTACAATTTCAGTGATAACACATGGGGAGAAAGATTTGATATGCCAAAAGAAATGGCACATTCACATCTAGGAATGGTAACCGACGGAAGGTACATATACATTGTCACAGGACAGTATGGGCCACAGTGCAGAGGGCCCACAGCTCATACGTTTGTGCTTGATACTGAGACAAAGCAGTGGCGGGACATGCCCCCTCTACCAGTTCCTAGGTATGCACCAGCAACTCAACTTTGGAAAGGTAGACTACATGTAATGGGTGGTAGTGGGGAGAACCGATATACACCTGAAGTGGACCATTGGAGTCTTGCTGTAAAAGATGGGAAAGTATTAGAAAAGGAATGGAGGACTGAAATTCCTATTCCCCGGGGAGGACCTCATAGGGCTTGTGTAGTTGTTGATGATCGTCTTTATCTTATCGGTGGTCAAGAGGGTGATTTCATGGCCAAACCCGGATCACCTATTTTCAAGTGCTCTCGTAGGAATGAGGTTGTATATAGTGATGTTTACATGATGGATGACGAAATGAAGTGGAAAGAGTTGCCTCCTATGCCTAAACCAGATTCCCATATTGAATTCGCTTGGACTGTTGTTAACAACTCTATTGTTATCGTTGGAGGCACAACTGAGAAGCACCCTGTAACTAAGAAGATGACTTTGGTTGGTGAAATATTCCAGTTCAACTTGAATACACTGTGGTCAGTTATTGGGAAACTTCCATTTCGAGTTAAAACCACACTAGTTGGTTTCTGGAATGGGTGGTTGTATTTTACATCCGGACAGCGAGACAAAGGACCGGATAATCCCCAACCAAAGAAGGTCATCGGAGAGTTGTGGAGGACGAAATTAAAACTGAACTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006091 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009061 GO:0009987 GO:0010498 GO:0012505 GO:0015980 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031410 GO:0031461 GO:0031625 GO:0031982 GO:0031984 GO:0032991 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0050789 GO:0051171 GO:0051246 GO:0051603 GO:0055114 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097708 GO:0098791 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_1 Kelch_4 Kelch_6

Protein Analysis

429

Amino Acids

48.27

Weight (kDa)

9.01

Isoelectric Point (pI)

28.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kelch_KLHDC2_KLHL20_DRC7	PF24681	106 - 206	8.1e-08	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	121 - 288	2.1e-08	Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7	PF24681	158 - 348	1.7e-18	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95	PF25210	162 - 290	4.6e-10	FKB95, Kelch-repeats domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51540
fragaria_vesca	FvH4_6g37100 FvH4_6g37100 FvH4_6g37100 FvH4_6g37100 FvH4_6g37100
malus_domestica	MD09G1161600.v1.1
prunus_persica	Prupe.3G020700_v2.0.a1 Prupe.3G020700_v2.0.a1 Prupe.3G020700_v2.0.a1 Prupe.3G020700_v2.0.a1
pyrus_communis	pycom09g08010 pycom09g08060 pycom17g14080 pycom17g14120
rosa_chinensis	RchiOBHm_Chr2g0149661
rosa_laevigata	RLG00000020435
rosa_roxburghii	Rroxscaffold_2G00097820
rosa_rugosa	Rorug02G0414300
rosa_samantha	Rh2AG474400 Rh2BG486100 Rh2DG495400
rosa_wichuraiana	Rw2G038680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	123
Acc65I	GGTACC	1 cut(s)	392
AccB1I	GGYRCC	2 cut(s)	322, 392
AccI	GTMKAC	1 cut(s)	667
AccII	CGCG	1 cut(s)	17
AccIII	TCCGGA	1 cut(s)	1199
AciI	CCGC	2 cut(s)	15, 607
AclWI	GGATC	1 cut(s)	886
AcoI	YGGCCR	1 cut(s)	867
AcsI	RAATTY	2 cut(s)	777, 998
AcuI	CTGAAG	1 cut(s)	732
AfaI	GTAC	4 cut(s)	330, 394, 408, 518
AfiI	CCNNNNNNNGG	5 cut(s)	141, 217, 218, 513, 794
AflIII	ACRYGT	1 cut(s)	673
AgsI	TTSAA	4 cut(s)	892, 998, 1105, 1111
AhlI	ACTAGT	1 cut(s)	1162
AjnI	CCWGG	2 cut(s)	250, 286
AjuI	GAANNNNNNNTTGG	2 cut(s)	1064, 1096
AluBI	AGCT	1 cut(s)	569
AluI	AGCT	1 cut(s)	569
Alw21I	GWGCWC	1 cut(s)	899
Alw26I	GTCTC	2 cut(s)	587, 1203
AlwI	GGATC	1 cut(s)	886
Ama87I	CYCGRG	1 cut(s)	789
Aor13HI	TCCGGA	1 cut(s)	1199
AoxI	GGCC	4 cut(s)	8, 545, 560, 867
ApaI	GGGCCC	1 cut(s)	564
ApeKI	GCWGC	2 cut(s)	37, 347
ApoI	RAATTY	2 cut(s)	777, 998
Asp718I	GGTACC	1 cut(s)	392
AspA2I	CCTAGG	1 cut(s)	632
AspS9I	GGNCC	8 cut(s)	9, 290, 545, 560, 561, 718, 797, 1217
AsuC2I	CCSGG	3 cut(s)	790, 791, 876
AsuHPI	GGTGA	3 cut(s)	869, 873, 1100
AvaI	CYCGRG	1 cut(s)	789
AvaII	GGWCC	4 cut(s)	290, 718, 797, 1217
AvrII	CCTAGG	1 cut(s)	632
BaeGI	GKGCMC	1 cut(s)	564
BalI	TGGCCA	1 cut(s)	869
BanI	GGYRCC	2 cut(s)	322, 392
BanII	GRGCYC	2 cut(s)	148, 564
BauI	CACGAG	1 cut(s)	1282
Bbv12I	GWGCWC	1 cut(s)	899
BbvCI	CCTCAGC	1 cut(s)	318
BbvI	GCAGC	2 cut(s)	24, 334
BccI	CCATC	3 cut(s)	335, 737, 931
BciT130I	CCWGG	2 cut(s)	252, 288
BcnI	CCSGG	3 cut(s)	790, 791, 876
BcoDI	GTCTC	2 cut(s)	587, 1203
BcuI	ACTAGT	1 cut(s)	1162
BfaI	CTAG	5 cut(s)	174, 198, 494, 633, 1163
BisI	GCNGC	2 cut(s)	38, 348
BlnI	CCTAGG	1 cut(s)	632
BlsI	GCNGC	2 cut(s)	39, 349
Bme1390I	CCNGG	5 cut(s)	252, 288, 790, 791, 876
Bme18I	GGWCC	4 cut(s)	290, 718, 797, 1217
BmeT110I	CYCGRG	1 cut(s)	789
BmgT120I	GGNCC	8 cut(s)	9, 290, 545, 560, 561, 718, 797, 1217
BmiI	GGNNCC	4 cut(s)	147, 324, 394, 562
BmrFI	CCNGG	5 cut(s)	252, 288, 790, 791, 876
BmsI	GCATC	1 cut(s)	186
Bpu10I	CCTNAGC	1 cut(s)	318
BpuMI	CCSGG	3 cut(s)	790, 791, 876
BsaJI	CCNNGG	5 cut(s)	216, 632, 788, 789, 790
BsaWI	WCCGGW	2 cut(s)	1199, 1219
Bsc4I	CCNNNNNNNGG	5 cut(s)	141, 217, 218, 513, 794
Bse1I	ACTGG	2 cut(s)	626, 1099
Bse3DI	GCAATG	1 cut(s)	129
BseAI	TCCGGA	1 cut(s)	1199
BseBI	CCWGG	2 cut(s)	252, 288
BseDI	CCNNGG	5 cut(s)	216, 632, 788, 789, 790
BseGI	GGATG	4 cut(s)	177, 346, 946, 1196
BseLI	CCNNNNNNNGG	5 cut(s)	141, 217, 218, 513, 794
BseMI	GCAATG	1 cut(s)	129
BseMII	CTCAG	3 cut(s)	309, 582, 1041
BseNI	ACTGG	2 cut(s)	626, 1099
BseRI	GAGGAG	2 cut(s)	93, 138
BseSI	GKGCMC	1 cut(s)	564
BseXI	GCAGC	2 cut(s)	24, 334
BseYI	CCCAGC	1 cut(s)	61
BsgI	GTGCAG	1 cut(s)	574
Bsh1236I	CGCG	1 cut(s)	17
BshFI	GGCC	4 cut(s)	10, 547, 562, 869
BshNI	GGYRCC	2 cut(s)	322, 392
BsiHKAI	GWGCWC	1 cut(s)	899
BsiHKCI	CYCGRG	1 cut(s)	789
BsiSI	CCGG	4 cut(s)	790, 876, 1200, 1220
BslFI	GGGAC	1 cut(s)	623
BslI	CCNNNNNNNGG	5 cut(s)	141, 217, 218, 513, 794
BsmAI	GTCTC	2 cut(s)	587, 1203
BsmFI	GGGAC	1 cut(s)	623
BsnI	GGCC	4 cut(s)	10, 547, 562, 869
BsoBI	CYCGRG	1 cut(s)	789
Bsp120I	GGGCCC	1 cut(s)	560
Bsp1286I	GDGCHC	3 cut(s)	148, 564, 899
Bsp13I	TCCGGA	1 cut(s)	1199
Bsp1407I	TGTACA	1 cut(s)	406
Bsp143I	GATC	2 cut(s)	826, 878
BspACI	CCGC	2 cut(s)	15, 607
BspANI	GGCC	4 cut(s)	10, 547, 562, 869
BspCNI	CTCAG	3 cut(s)	310, 583, 1042
BspEI	TCCGGA	1 cut(s)	1199
BspFNI	CGCG	1 cut(s)	17
BspLI	GGNNCC	4 cut(s)	147, 324, 394, 562
BspPI	GGATC	1 cut(s)	886
BspT107I	GGYRCC	2 cut(s)	322, 392
BsrDI	GCAATG	1 cut(s)	129
BsrGI	TGTACA	1 cut(s)	406
BsrI	ACTGG	2 cut(s)	626, 1099
BssECI	CCNNGG	5 cut(s)	216, 632, 788, 789, 790
BssMI	GATC	2 cut(s)	826, 878
BssSI	CACGAG	1 cut(s)	1282
BssT1I	CCWWGG	2 cut(s)	216, 632
Bst2BI	CACGAG	1 cut(s)	1282
Bst2UI	CCWGG	2 cut(s)	252, 288
Bst4CI	ACNGT	4 cut(s)	540, 552, 1012, 1119
Bst6I	CTCTTC	2 cut(s)	250, 377
BstAUI	TGTACA	1 cut(s)	406
BstDEI	CTNAG	5 cut(s)	189, 318, 591, 1050, 1068
BstEII	GGTNACC	1 cut(s)	502
BstF5I	GGATG	4 cut(s)	177, 346, 946, 1196
BstFNI	CGCG	1 cut(s)	17
BstKTI	GATC	2 cut(s)	829, 881
BstMAI	GTCTC	2 cut(s)	587, 1203
BstMBI	GATC	2 cut(s)	826, 878
BstMWI	GCNNNNNNNGC	4 cut(s)	77, 275, 613, 973
BstNI	CCWGG	2 cut(s)	252, 288
BstNSI	RCATGY	3 cut(s)	419, 616, 677
BstPI	GGTNACC	1 cut(s)	502
BstSCI	CCNGG	5 cut(s)	250, 286, 788, 789, 874
BstSLI	GKGCMC	1 cut(s)	564
BstUI	CGCG	1 cut(s)	17
BstV1I	GCAGC	2 cut(s)	24, 334
BsuRI	GGCC	4 cut(s)	10, 547, 562, 869
BtsCI	GGATG	4 cut(s)	177, 346, 946, 1196
BtsI	GCAGTG	1 cut(s)	608
BtsIMutI	CAGTG	4 cut(s)	442, 557, 608, 1115
Cfr13I	GGNCC	8 cut(s)	9, 290, 545, 560, 561, 718, 797, 1217
Cfr9I	CCCGGG	1 cut(s)	789
CseI	GACGC	2 cut(s)	40, 214
Csp6I	GTAC	4 cut(s)	329, 393, 407, 517
CspCI	CAANNNNNGTGG	2 cut(s)	1147, 1182
CviAII	CATG	7 cut(s)	203, 416, 447, 613, 674, 865, 934
CviQI	GTAC	4 cut(s)	329, 393, 407, 517
DdeI	CTNAG	5 cut(s)	189, 318, 591, 1050, 1068
DpnI	GATC	2 cut(s)	828, 880
DpnII	GATC	2 cut(s)	826, 878
EaeI	YGGCCR	1 cut(s)	867
Eam1104I	CTCTTC	2 cut(s)	250, 377
EarI	CTCTTC	2 cut(s)	250, 377
Eco130I	CCWWGG	2 cut(s)	216, 632
Eco24I	GRGCYC	2 cut(s)	148, 564
Eco32I	GATATC	2 cut(s)	160, 424
Eco47I	GGWCC	4 cut(s)	290, 718, 797, 1217
Eco57I	CTGAAG	1 cut(s)	732
Eco88I	CYCGRG	1 cut(s)	789
Eco91I	GGTNACC	1 cut(s)	502
EcoO109I	RGGNCCY	3 cut(s)	9, 560, 797
EcoO65I	GGTNACC	1 cut(s)	502
EcoRI	GAATTC	1 cut(s)	998
EcoRII	CCWGG	2 cut(s)	250, 286
EcoRV	GATATC	2 cut(s)	160, 424
EcoT14I	CCWWGG	2 cut(s)	216, 632
EcoT38I	GRGCYC	2 cut(s)	148, 564
ErhI	CCWWGG	2 cut(s)	216, 632
FaeI	CATG	7 cut(s)	206, 419, 450, 616, 677, 868, 937
FaqI	GGGAC	1 cut(s)	623
FatI	CATG	7 cut(s)	202, 415, 446, 612, 673, 864, 933
FauI	CCCGC	1 cut(s)	600
FblI	GTMKAC	1 cut(s)	667
Fnu4HI	GCNGC	2 cut(s)	38, 348
FokI	GGATG	4 cut(s)	164, 353, 953, 1183
FriOI	GRGCYC	2 cut(s)	148, 564
Fsp4HI	GCNGC	2 cut(s)	38, 348
FspBI	CTAG	5 cut(s)	174, 198, 494, 633, 1163
GluI	GCNGC	2 cut(s)	38, 348
GsaI	CCCAGC	1 cut(s)	65
HaeIII	GGCC	4 cut(s)	10, 547, 562, 869
HapII	CCGG	4 cut(s)	790, 876, 1200, 1220
HgaI	GACGC	2 cut(s)	40, 214
Hin1II	CATG	7 cut(s)	206, 419, 450, 616, 677, 868, 937
HincII	GTYRAC	1 cut(s)	1018
HindII	GTYRAC	1 cut(s)	1018
HinfI	GANTC	3 cut(s)	364, 727, 986
HpaI	GTTAAC	1 cut(s)	1018
HpaII	CCGG	4 cut(s)	790, 876, 1200, 1220
HphI	GGTGA	3 cut(s)	869, 873, 1100
Hpy166II	GTNNAC	4 cut(s)	668, 718, 931, 1018
Hpy188I	TCNGA	2 cut(s)	75, 1250
Hpy188III	TCNNGA	4 cut(s)	851, 1174, 1200, 1284
Hpy8I	GTNNAC	4 cut(s)	668, 718, 931, 1018
Hpy99I	CGWCG	3 cut(s)	56, 230, 512
HpyAV	CCTTC	4 cut(s)	283, 302, 507, 1234
HpyCH4III	ACNGT	4 cut(s)	540, 552, 1012, 1119
HpyCH4IV	ACGT	1 cut(s)	575
HpyCH4V	TGCA	2 cut(s)	555, 641
HpyF10VI	GCNNNNNNNGC	4 cut(s)	77, 275, 613, 973
HpyF3I	CTNAG	5 cut(s)	189, 318, 591, 1050, 1068
HpySE526I	ACGT	1 cut(s)	575
Hsp92II	CATG	7 cut(s)	206, 419, 450, 616, 677, 868, 937
Kpn2I	TCCGGA	1 cut(s)	1199
KpnI	GGTACC	1 cut(s)	396
KspAI	GTTAAC	1 cut(s)	1018
Kzo9I	GATC	2 cut(s)	826, 878
LmnI	GCTCC	2 cut(s)	151, 253
Lsp1109I	GCAGC	2 cut(s)	24, 334
LweI	GCATC	1 cut(s)	186
MaeI	CTAG	5 cut(s)	174, 198, 494, 633, 1163
MaeII	ACGT	1 cut(s)	575
MaeIII	GTNAC	3 cut(s)	502, 529, 1063
MalI	GATC	2 cut(s)	828, 880
MboI	GATC	2 cut(s)	826, 878
MboII	GAAGA	4 cut(s)	267, 359, 364, 1084
MfeI	CAATTG	1 cut(s)	139
MhlI	GDGCHC	3 cut(s)	148, 564, 899
MlsI	TGGCCA	1 cut(s)	869
MluCI	AATT	6 cut(s)	139, 296, 430, 777, 998, 1268
MluNI	TGGCCA	1 cut(s)	869
MlyI	GAGTC	1 cut(s)	736
MmeI	TCCRAC	1 cut(s)	1018
Mox20I	TGGCCA	1 cut(s)	869
MroI	TCCGGA	1 cut(s)	1199
MscI	TGGCCA	1 cut(s)	869
MseI	TTAA	4 cut(s)	299, 1017, 1152, 1271
MslI	CAYNNNNRTG	1 cut(s)	414
Msp20I	TGGCCA	1 cut(s)	869
MspI	CCGG	4 cut(s)	790, 876, 1200, 1220
MspR9I	CCNGG	5 cut(s)	252, 288, 790, 791, 876
MunI	CAATTG	1 cut(s)	139
MvaI	CCWGG	2 cut(s)	252, 288
MvnI	CGCG	1 cut(s)	17
MwoI	GCNNNNNNNGC	4 cut(s)	77, 275, 613, 973
NciI	CCSGG	3 cut(s)	790, 791, 876
NdeII	GATC	2 cut(s)	826, 878
NlaIII	CATG	7 cut(s)	206, 419, 450, 616, 677, 868, 937
NlaIV	GGNNCC	4 cut(s)	147, 324, 394, 562
NmeAIII	GCCGAG	1 cut(s)	13
NmuCI	GTSAC	1 cut(s)	529
NspI	RCATGY	3 cut(s)	419, 616, 677
PciI	ACATGT	1 cut(s)	673
PcsI	WCGNNNNNNNCGW	1 cut(s)	112
PfeI	GAWTC	2 cut(s)	364, 986
PfoI	TCCNGGA	1 cut(s)	250
PkrI	GCNGC	2 cut(s)	39, 349
PleI	GAGTC	1 cut(s)	735
PpsI	GAGTC	1 cut(s)	735
PpuMI	RGGWCCY	1 cut(s)	797
PscI	ACATGT	1 cut(s)	673
PsiI	TTATAA	1 cut(s)	123
Psp5II	RGGWCCY	1 cut(s)	797
Psp6I	CCWGG	2 cut(s)	250, 286
PspEI	GGTNACC	1 cut(s)	502
PspFI	CCCAGC	1 cut(s)	61
PspGI	CCWGG	2 cut(s)	250, 286
PspN4I	GGNNCC	4 cut(s)	147, 324, 394, 562
PspOMI	GGGCCC	1 cut(s)	560
PspPI	GGNCC	8 cut(s)	9, 290, 545, 560, 561, 718, 797, 1217
PspPPI	RGGWCCY	1 cut(s)	797
RsaI	GTAC	4 cut(s)	330, 394, 408, 518
RsaNI	GTAC	4 cut(s)	329, 393, 407, 517
RseI	CAYNNNNRTG	1 cut(s)	414
SaqAI	TTAA	4 cut(s)	299, 1017, 1152, 1271
SatI	GCNGC	2 cut(s)	38, 348
Sau3AI	GATC	2 cut(s)	826, 878
Sau96I	GGNCC	8 cut(s)	9, 290, 545, 560, 561, 718, 797, 1217
SchI	GAGTC	1 cut(s)	736
ScrFI	CCNGG	5 cut(s)	252, 288, 790, 791, 876
SduI	GDGCHC	3 cut(s)	148, 564, 899
SfaNI	GCATC	1 cut(s)	186
SinI	GGWCC	4 cut(s)	290, 718, 797, 1217
SmaI	CCCGGG	1 cut(s)	791
SmiMI	CAYNNNNRTG	1 cut(s)	414
SpeI	ACTAGT	1 cut(s)	1162
Sse9I	AATT	6 cut(s)	139, 296, 430, 777, 998, 1268
SsiI	CCGC	2 cut(s)	15, 607
SspI	AATATT	1 cut(s)	1095
SspMI	CTAG	5 cut(s)	174, 198, 494, 633, 1163
StyD4I	CCNGG	5 cut(s)	250, 286, 788, 789, 874
StyI	CCWWGG	2 cut(s)	216, 632
TaaI	ACNGT	4 cut(s)	540, 552, 1012, 1119
TaiI	ACGT	1 cut(s)	578
TaqI	TCGA	2 cut(s)	228, 1147
TaqII	GACCGA	1 cut(s)	44
TasI	AATT	6 cut(s)	139, 296, 430, 777, 998, 1268
TatI	WGTACW	1 cut(s)	406
TfiI	GAWTC	2 cut(s)	364, 986
Tru1I	TTAA	4 cut(s)	299, 1017, 1152, 1271
Tru9I	TTAA	4 cut(s)	299, 1017, 1152, 1271
TscAI	CASTG	4 cut(s)	442, 557, 608, 1122
TseFI	GTSAC	1 cut(s)	529
TseI	GCWGC	2 cut(s)	37, 347
Tsp45I	GTSAC	1 cut(s)	529
TspDTI	ATGAA	3 cut(s)	219, 853, 965
TspGWI	ACGGA	2 cut(s)	104, 525
TspMI	CCCGGG	1 cut(s)	789
TspRI	CASTG	4 cut(s)	442, 557, 608, 1122
VpaK11BI	GGWCC	4 cut(s)	290, 718, 797, 1217
XapI	RAATTY	2 cut(s)	777, 998
XceI	RCATGY	3 cut(s)	419, 616, 677
XcmI	CCANNNNNNNNNTGG	1 cut(s)	300
XmaI	CCCGGG	1 cut(s)	789
XmaJI	CCTAGG	1 cut(s)	632
XmiI	GTMKAC	1 cut(s)	667
XspI	CTAG	5 cut(s)	174, 198, 494, 633, 1163

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.