Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0008313

Physical Location & Seq

Genomic Coordinates Forward (+)

71540522 .. 71545590

Gene Structure 5069 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG495400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1254 bp

ATGGTTAGAGCAGCTGGGAAGCACACATCGGCTCGTTTGGTCTTGATTTGCGTTGGCCTTCTGGGTATTGCTCTCATTGCTGATTTCCTCTGGGCTTCTTCTTCTTCTTCTCGTTTTGCTCTCATTGGCGCCAATTGGGCTCCTCCTGTTTCGCTCCTCACCCCCATAACCATACCCAACAAGGGAGTAGACAGTCAAAAGAACAATACAACTACCGGGAGGGTTTTGTCTGCAACTTTTGCTGATTTGCCTGCACCAGAACTAAAATGGGAAAAGATGGCTGCCGCACCTGTGCCTCGTTTGGATGGAGCTGCTATGCAGATTAAGAATCTTCTATACGTGTTTGCTGGATACGGTACAATTGATTATGTACATTCACATGTTGACATCTACAATTTCAGTGATAATACATGGGGAGGAAGATTTGATATGCCGAAAGAAATGGCACATTCTCATCTAGGAATGGTAACTGATGGAAGGTACATATACATTGTCAATGGACAGTATGGGCCACAGTGCAGAGGCCCCACATCTCATACATTCATCCTTGATACTGAGACAAAGAAGTGGCAGAATATGCCCCCATTACCAGTTCCAAGGTATGCACCAGCAACTCAACTTTGGAGAGGTAGACTCCATGTAATGGGCGGTAGCAAGGAAAATCGACATACACCTGCAGTAGACCATTGGAGTCTTGCTGTAAAAGATGGAAAAGCATTAGAAAAGGAATGGAGGACTGAAGTGCCTATTCCCAAGGGAGGACCTCATAGGGCTTCTATAGTTTATAATGATCGTCTTTATGTTATGGGTGGTCAAGAGGGTGATTTCATGGCAAAACCTGGATCACCTATTTTCAAGTGCTCTCGTAGGAATGAGGTTGTATATGGCGATGTTTACATGCTGGATGATGAAATGAAGTGGAAAGTGTTACCTCCTATGCCTAAGCCAGATTCCCATATTGAATTTGCTTGGGCTCTTGTTGACAATTCTGTTATTATTGTTGGAGGCACAACAGAGAAGCACCCTATAACTAAGAAGATGGTTTTGGTTGGAGAAATGTTCCAGTTTAACTTCGATACTCTGAACTGGTCAGTCATAGGGAAACTACCATTTCGAGTTAAAACCACGCTAGTAGGTTTCTGGAAAGGGTGGTTGTATTTCACATCAGGACAGCGGGACAAAGGACCAGAGGATCCAGCACCAAAGAAAGTGATTGGAGAACTGTGGAGAACAAAGTTAAACCTGGGCTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006091 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009061 GO:0009987 GO:0010498 GO:0012505 GO:0015980 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031410 GO:0031461 GO:0031625 GO:0031982 GO:0031984 GO:0032991 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0050789 GO:0051171 GO:0051246 GO:0051603 GO:0055114 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097708 GO:0098791 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_1 Kelch_4 Kelch_6

Protein Analysis

417

Amino Acids

46.55

Weight (kDa)

9.36

Isoelectric Point (pI)

27.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kelch_KLHDC2_KLHL20_DRC7	PF24681	88 - 194	3.1e-08	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	91 - 236	5.7e-07	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	108 - 276	3.8e-09	Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7	PF24681	146 - 336	1.6e-15	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95	PF25210	150 - 278	4.1e-11	FKB95, Kelch-repeats domain
Beta-prop_FBX42	PF13415	153 - 275	1.1e-06	FBX42, beta-propeller domain
Beta-prop_FBX42	PF13415	210 - 365	1.8e-08	FBX42, beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51540
fragaria_vesca	FvH4_6g37100 FvH4_6g37100 FvH4_6g37100 FvH4_6g37100 FvH4_6g37100
malus_domestica	MD09G1161600.v1.1
prunus_persica	Prupe.3G020700_v2.0.a1 Prupe.3G020700_v2.0.a1 Prupe.3G020700_v2.0.a1 Prupe.3G020700_v2.0.a1
pyrus_communis	pycom09g08010 pycom09g08060 pycom17g14080 pycom17g14120
rosa_chinensis	RchiOBHm_Chr2g0149661
rosa_laevigata	RLG00000020435
rosa_roxburghii	Rroxscaffold_2G00097820
rosa_rugosa	Rorug02G0414300
rosa_samantha	Rh2AG474400 Rh2BG486100 Rh2DG495400
rosa_wichuraiana	Rw2G038680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	786
AarI	CACCTGC	1 cut(s)	682
Acc36I	ACCTGC	1 cut(s)	682
AccB1I	GGYRCC	1 cut(s)	128
AccB7I	CCANNNNNTGG	1 cut(s)	643
AccI	GTMKAC	3 cut(s)	189, 631, 681
AciI	CCGC	3 cut(s)	285, 648, 1174
AclWI	GGATC	3 cut(s)	850, 1187, 1200
AcsI	RAATTY	1 cut(s)	962
AcuI	CTGAAG	1 cut(s)	759
AcyI	GRCGYC	1 cut(s)	129
AfaI	GTAC	3 cut(s)	358, 372, 482
AfiI	CCNNNNNNNGG	3 cut(s)	182, 643, 758
AflIII	ACRYGT	2 cut(s)	339, 379
AgsI	TTSAA	2 cut(s)	856, 962
AjnI	CCWGG	2 cut(s)	838, 1242
AjuI	GAANNNNNNNTTGG	2 cut(s)	1028, 1060
AluBI	AGCT	2 cut(s)	14, 311
AluI	AGCT	2 cut(s)	14, 311
Alw21I	GWGCWC	1 cut(s)	863
Alw26I	GTCTC	1 cut(s)	551
AlwI	GGATC	3 cut(s)	850, 1187, 1200
AoxI	GGCC	3 cut(s)	55, 509, 523
ApeKI	GCWGC	3 cut(s)	11, 281, 311
ApoI	RAATTY	1 cut(s)	962
AspLEI	GCGC	1 cut(s)	131
AspS9I	GGNCC	4 cut(s)	509, 524, 761, 1184
AsuC2I	CCSGG	1 cut(s)	217
AsuHPI	GGTGA	3 cut(s)	151, 833, 837
AvaII	GGWCC	2 cut(s)	761, 1184
BamHI	GGATCC	1 cut(s)	1192
BanI	GGYRCC	1 cut(s)	128
BanII	GRGCYC	3 cut(s)	142, 976, 1250
BauI	CACGAG	1 cut(s)	1249
Bbv12I	GWGCWC	1 cut(s)	863
BbvI	GCAGC	3 cut(s)	23, 268, 298
BccI	CCATC	5 cut(s)	271, 299, 467, 701, 1033
BciT130I	CCWGG	2 cut(s)	840, 1244
BciVI	GTATCC	1 cut(s)	344
BcnI	CCSGG	1 cut(s)	217
BcoDI	GTCTC	1 cut(s)	551
BfaI	CTAG	2 cut(s)	458, 1130
BfmI	CTRYAG	2 cut(s)	675, 777
BfoI	RGCGCY	1 cut(s)	132
BfuAI	ACCTGC	1 cut(s)	682
BfuI	GTATCC	1 cut(s)	344
BglI	GCCNNNNNGGC	1 cut(s)	137
BisI	GCNGC	4 cut(s)	12, 282, 285, 312
BlsI	GCNGC	4 cut(s)	13, 283, 286, 313
Bme1390I	CCNGG	3 cut(s)	217, 840, 1244
Bme18I	GGWCC	2 cut(s)	761, 1184
BmgT120I	GGNCC	4 cut(s)	509, 524, 761, 1184
BmiI	GGNNCC	4 cut(s)	130, 141, 526, 1194
BmrFI	CCNGG	3 cut(s)	217, 840, 1244
BplI	GAGNNNNNCTC	2 cut(s)	618, 650
Bpu10I	CCTNAGC	1 cut(s)	942
BpuMI	CCSGG	1 cut(s)	217
BsaAI	YACGTR	1 cut(s)	340
BsaHI	GRCGYC	1 cut(s)	129
BsaJI	CCNNGG	3 cut(s)	596, 753, 1243
Bsc4I	CCNNNNNNNGG	3 cut(s)	182, 643, 758
Bse1I	ACTGG	3 cut(s)	590, 1063, 1091
Bse3DI	GCAATG	1 cut(s)	75
BseBI	CCWGG	2 cut(s)	840, 1244
BseDI	CCNNGG	3 cut(s)	596, 753, 1243
BseGI	GGATG	3 cut(s)	310, 543, 910
BseLI	CCNNNNNNNGG	3 cut(s)	182, 643, 758
BseMI	GCAATG	1 cut(s)	75
BseMII	CTCAG	1 cut(s)	546
BseNI	ACTGG	3 cut(s)	590, 1063, 1091
BseRI	GAGGAG	2 cut(s)	132, 146
BseXI	GCAGC	3 cut(s)	23, 268, 298
BseYI	CCCAGC	1 cut(s)	14
BsgI	GTGCAG	2 cut(s)	237, 538
BshFI	GGCC	3 cut(s)	57, 511, 525
BshNI	GGYRCC	1 cut(s)	128
BsiHKAI	GWGCWC	1 cut(s)	863
BsiSI	CCGG	1 cut(s)	216
BslFI	GGGAC	1 cut(s)	1190
BslI	CCNNNNNNNGG	3 cut(s)	182, 643, 758
BsmAI	GTCTC	1 cut(s)	551
BsmFI	GGGAC	1 cut(s)	1190
BsnI	GGCC	3 cut(s)	57, 511, 525
Bsp1286I	GDGCHC	4 cut(s)	142, 863, 976, 1250
Bsp1407I	TGTACA	1 cut(s)	370
Bsp143I	GATC	3 cut(s)	790, 842, 1192
BspACI	CCGC	3 cut(s)	285, 648, 1174
BspANI	GGCC	3 cut(s)	57, 511, 525
BspCNI	CTCAG	1 cut(s)	547
BspLI	GGNNCC	4 cut(s)	130, 141, 526, 1194
BspMAI	CTGCAG	1 cut(s)	679
BspMI	ACCTGC	1 cut(s)	682
BspPI	GGATC	3 cut(s)	850, 1187, 1200
BspT107I	GGYRCC	1 cut(s)	128
BsrDI	GCAATG	1 cut(s)	75
BsrGI	TGTACA	1 cut(s)	370
BsrI	ACTGG	3 cut(s)	590, 1063, 1091
BssECI	CCNNGG	3 cut(s)	596, 753, 1243
BssMI	GATC	3 cut(s)	790, 842, 1192
BssNI	GRCGYC	1 cut(s)	129
BssSI	CACGAG	1 cut(s)	1249
BssT1I	CCWWGG	2 cut(s)	596, 753
Bst2BI	CACGAG	1 cut(s)	1249
Bst2UI	CCWGG	2 cut(s)	840, 1244
Bst4CI	ACNGT	5 cut(s)	194, 356, 504, 516, 1224
BstACI	GRCGYC	1 cut(s)	129
BstAPI	GCANNNNNTGC	2 cut(s)	239, 577
BstAUI	TGTACA	1 cut(s)	370
BstBAI	YACGTR	1 cut(s)	340
BstC8I	GCNNGC	1 cut(s)	252
BstDEI	CTNAG	3 cut(s)	555, 942, 1032
BstF5I	GGATG	3 cut(s)	310, 543, 910
BstH2I	RGCGCY	1 cut(s)	132
BstHHI	GCGC	1 cut(s)	131
BstKTI	GATC	3 cut(s)	793, 845, 1195
BstMAI	GTCTC	1 cut(s)	551
BstMBI	GATC	3 cut(s)	790, 842, 1192
BstMWI	GCNNNNNNNGC	4 cut(s)	77, 137, 239, 577
BstNI	CCWGG	2 cut(s)	840, 1244
BstNSI	RCATGY	2 cut(s)	383, 901
BstSCI	CCNGG	3 cut(s)	215, 838, 1242
BstSFI	CTRYAG	2 cut(s)	675, 777
BstV1I	GCAGC	3 cut(s)	23, 268, 298
BstX2I	RGATCY	1 cut(s)	1192
BstYI	RGATCY	1 cut(s)	1192
BsuI	GTATCC	1 cut(s)	344
BsuRI	GGCC	3 cut(s)	57, 511, 525
BtgZI	GCGATG	1 cut(s)	903
BtsCI	GGATG	3 cut(s)	310, 543, 910
BtsIMutI	CAGTG	2 cut(s)	406, 521
BveI	ACCTGC	1 cut(s)	682
Cac8I	GCNNGC	1 cut(s)	252
CfoI	GCGC	1 cut(s)	131
Cfr13I	GGNCC	4 cut(s)	509, 524, 761, 1184
Csp6I	GTAC	3 cut(s)	357, 371, 481
CviAII	CATG	5 cut(s)	380, 411, 638, 829, 898
CviQI	GTAC	3 cut(s)	357, 371, 481
DdeI	CTNAG	3 cut(s)	555, 942, 1032
DinI	GGCGCC	1 cut(s)	130
DpnI	GATC	3 cut(s)	792, 844, 1194
DpnII	GATC	3 cut(s)	790, 842, 1192
Eco130I	CCWWGG	2 cut(s)	596, 753
Eco24I	GRGCYC	3 cut(s)	142, 976, 1250
Eco47I	GGWCC	2 cut(s)	761, 1184
Eco57I	CTGAAG	1 cut(s)	759
EcoO109I	RGGNCCY	2 cut(s)	524, 761
EcoRII	CCWGG	2 cut(s)	838, 1242
EcoT14I	CCWWGG	2 cut(s)	596, 753
EcoT38I	GRGCYC	3 cut(s)	142, 976, 1250
EgeI	GGCGCC	1 cut(s)	130
EheI	GGCGCC	1 cut(s)	130
ErhI	CCWWGG	2 cut(s)	596, 753
FaeI	CATG	5 cut(s)	383, 414, 641, 832, 901
FaqI	GGGAC	1 cut(s)	1190
FatI	CATG	5 cut(s)	379, 410, 637, 828, 897
FauI	CCCGC	1 cut(s)	1167
FblI	GTMKAC	3 cut(s)	189, 631, 681
Fnu4HI	GCNGC	4 cut(s)	12, 282, 285, 312
FokI	GGATG	3 cut(s)	317, 530, 917
FriOI	GRGCYC	3 cut(s)	142, 976, 1250
Fsp4HI	GCNGC	4 cut(s)	12, 282, 285, 312
FspBI	CTAG	2 cut(s)	458, 1130
GlaI	GCGC	1 cut(s)	130
GluI	GCNGC	4 cut(s)	12, 282, 285, 312
GsaI	CCCAGC	1 cut(s)	18
HaeII	RGCGCY	1 cut(s)	132
HaeIII	GGCC	3 cut(s)	57, 511, 525
HapII	CCGG	1 cut(s)	216
HhaI	GCGC	1 cut(s)	131
Hin1I	GRCGYC	1 cut(s)	129
Hin1II	CATG	5 cut(s)	383, 414, 641, 832, 901
Hin6I	GCGC	1 cut(s)	129
HinP1I	GCGC	1 cut(s)	129
HincII	GTYRAC	2 cut(s)	385, 982
HindII	GTYRAC	2 cut(s)	385, 982
HinfI	GANTC	4 cut(s)	328, 633, 691, 950
HpaII	CCGG	1 cut(s)	216
HphI	GGTGA	3 cut(s)	151, 833, 837
Hpy166II	GTNNAC	6 cut(s)	190, 385, 632, 682, 895, 982
Hpy188I	TCNGA	1 cut(s)	1083
Hpy188III	TCNNGA	5 cut(s)	43, 815, 1141, 1167, 1251
Hpy8I	GTNNAC	6 cut(s)	190, 385, 632, 682, 895, 982
HpyAV	CCTTC	2 cut(s)	68, 471
HpyCH4III	ACNGT	5 cut(s)	194, 356, 504, 516, 1224
HpyCH4IV	ACGT	1 cut(s)	339
HpyCH4V	TGCA	6 cut(s)	233, 254, 319, 519, 605, 677
HpyF10VI	GCNNNNNNNGC	4 cut(s)	77, 137, 239, 577
HpyF3I	CTNAG	3 cut(s)	555, 942, 1032
HpySE526I	ACGT	1 cut(s)	339
Hsp92I	GRCGYC	1 cut(s)	129
Hsp92II	CATG	5 cut(s)	383, 414, 641, 832, 901
HspAI	GCGC	1 cut(s)	129
KasI	GGCGCC	1 cut(s)	128
Kzo9I	GATC	3 cut(s)	790, 842, 1192
LmnI	GCTCC	3 cut(s)	145, 159, 308
Lsp1109I	GCAGC	3 cut(s)	23, 268, 298
MaeI	CTAG	2 cut(s)	458, 1130
MaeII	ACGT	1 cut(s)	339
MaeIII	GTNAC	2 cut(s)	466, 927
MalI	GATC	3 cut(s)	792, 844, 1194
MboI	GATC	3 cut(s)	790, 842, 1192
MboII	GAAGA	7 cut(s)	90, 93, 96, 99, 323, 432, 1048
MfeI	CAATTG	2 cut(s)	133, 360
MflI	RGATCY	1 cut(s)	1192
MhlI	GDGCHC	4 cut(s)	142, 863, 976, 1250
MluCI	AATT	5 cut(s)	133, 360, 394, 962, 985
Mly113I	GGCGCC	1 cut(s)	129
MlyI	GAGTC	2 cut(s)	627, 700
MmeI	TCCRAC	2 cut(s)	982, 1030
MseI	TTAA	4 cut(s)	324, 1068, 1119, 1238
MslI	CAYNNNNRTG	1 cut(s)	378
MspA1I	CMGCKG	2 cut(s)	14, 1174
MspI	CCGG	1 cut(s)	216
MspR9I	CCNGG	3 cut(s)	217, 840, 1244
MunI	CAATTG	2 cut(s)	133, 360
MvaI	CCWGG	2 cut(s)	840, 1244
MwoI	GCNNNNNNNGC	4 cut(s)	77, 137, 239, 577
NarI	GGCGCC	1 cut(s)	129
NciI	CCSGG	1 cut(s)	217
NdeII	GATC	3 cut(s)	790, 842, 1192
NlaIII	CATG	5 cut(s)	383, 414, 641, 832, 901
NlaIV	GGNNCC	4 cut(s)	130, 141, 526, 1194
NspI	RCATGY	2 cut(s)	383, 901
PaqCI	CACCTGC	1 cut(s)	682
PciI	ACATGT	1 cut(s)	379
PfeI	GAWTC	2 cut(s)	328, 950
PflMI	CCANNNNNTGG	1 cut(s)	643
PkrI	GCNGC	4 cut(s)	13, 283, 286, 313
PleI	GAGTC	2 cut(s)	627, 699
PluTI	GGCGCC	1 cut(s)	132
PpsI	GAGTC	2 cut(s)	627, 699
Ppu21I	YACGTR	1 cut(s)	340
PpuMI	RGGWCCY	1 cut(s)	761
PscI	ACATGT	1 cut(s)	379
PsiI	TTATAA	1 cut(s)	786
Psp5II	RGGWCCY	1 cut(s)	761
Psp6I	CCWGG	2 cut(s)	838, 1242
PspFI	CCCAGC	1 cut(s)	14
PspGI	CCWGG	2 cut(s)	838, 1242
PspN4I	GGNNCC	4 cut(s)	130, 141, 526, 1194
PspPI	GGNCC	4 cut(s)	509, 524, 761, 1184
PspPPI	RGGWCCY	1 cut(s)	761
PstI	CTGCAG	1 cut(s)	679
PsuI	RGATCY	1 cut(s)	1192
PvuII	CAGCTG	1 cut(s)	14
RsaI	GTAC	3 cut(s)	358, 372, 482
RsaNI	GTAC	3 cut(s)	357, 371, 481
RseI	CAYNNNNRTG	1 cut(s)	378
SaqAI	TTAA	4 cut(s)	324, 1068, 1119, 1238
SatI	GCNGC	4 cut(s)	12, 282, 285, 312
Sau3AI	GATC	3 cut(s)	790, 842, 1192
Sau96I	GGNCC	4 cut(s)	509, 524, 761, 1184
SchI	GAGTC	2 cut(s)	627, 700
ScrFI	CCNGG	3 cut(s)	217, 840, 1244
SduI	GDGCHC	4 cut(s)	142, 863, 976, 1250
SfcI	CTRYAG	2 cut(s)	675, 777
SfoI	GGCGCC	1 cut(s)	130
SinI	GGWCC	2 cut(s)	761, 1184
SmiMI	CAYNNNNRTG	1 cut(s)	378
Sse9I	AATT	5 cut(s)	133, 360, 394, 962, 985
SsiI	CCGC	3 cut(s)	285, 648, 1174
SspDI	GGCGCC	1 cut(s)	128
SspMI	CTAG	2 cut(s)	458, 1130
StyD4I	CCNGG	3 cut(s)	215, 838, 1242
StyI	CCWWGG	2 cut(s)	596, 753
TaaI	ACNGT	5 cut(s)	194, 356, 504, 516, 1224
TaiI	ACGT	1 cut(s)	342
TaqI	TCGA	3 cut(s)	664, 1074, 1114
TasI	AATT	5 cut(s)	133, 360, 394, 962, 985
TatI	WGTACW	1 cut(s)	370
TauI	GCSGC	1 cut(s)	287
TfiI	GAWTC	2 cut(s)	328, 950
Tru1I	TTAA	4 cut(s)	324, 1068, 1119, 1238
Tru9I	TTAA	4 cut(s)	324, 1068, 1119, 1238
TscAI	CASTG	2 cut(s)	406, 521
TseI	GCWGC	3 cut(s)	11, 281, 311
TspDTI	ATGAA	4 cut(s)	532, 817, 924, 929
TspRI	CASTG	2 cut(s)	406, 521
Van91I	CCANNNNNTGG	1 cut(s)	643
VpaK11BI	GGWCC	2 cut(s)	761, 1184
XapI	RAATTY	1 cut(s)	962
XceI	RCATGY	2 cut(s)	383, 901
XcmI	CCANNNNNNNNNTGG	1 cut(s)	264
XmiI	GTMKAC	3 cut(s)	189, 631, 681
XspI	CTAG	2 cut(s)	458, 1130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.