MD09G1179400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007944
Physical Location & Seq
Reverse (-)
15356322 .. 15358546
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1179400.v1.1.491

Sequence Viewer

Length: 789 bp
ATGCAAAATAAGATCAATGGGAAGCAAGGAAACAAGAAACCAAATACAGTTTCTACTACAACCCATTCTGCAAAACAAGAGCCACGCGAAGAATTCAGTGACTGGCCTCATGGATTACTAGCAATTGGAACTTTTGGAAACAATGAATTGAAAGAAAAAAAAGCTGCTCAAAGCCAAGATATTCAGGAAGATCCGTCTTCATCCGAAGAAATCCTAGATAATTTCACATCAGAGGAAGTAGGTAAATTGCAAAAAGAGTTGACGAAACTCTTAACGCGAAAACCAGACATAGAAAAGCAAATCGCAGATCTTCCGTTGGATAGATTTCTAAATTGCCCGTCGAGCTTGGAGGTTGATCGGAGAACCAGCAACGCCCTTTGCTCCGATTCATCAGATGATCACAAGGATGAAGATATTGAGAAAACTATTAGTGTCATACTTGGTAGATGCAAAGACATTTATGCAGACAATAAGAAGGCAATTGGGAAGAAATCAATTTCTTTTCTTCTTAAGAAGATGTTCGTTTGTCGAAGTGGATTTGCACCAGCATCAAGTTTGAGAGATACACTTCAAGAGTCGAGAATGGAGAAGCTTCTTAGGGTAATGCTTAACAAGAACATCATCAATCCGCAAGGTGCTTCTCGAGCATCATCAATGAAGAAATACCTCGAGGACAGACAGAATCCAACAAAGAAGGGAAATAACAATGAAGATGATACAAAAGAGAAGATTAATGATGGATGTAAATGGGTCAAGACAGATTCTGAATATATAGTACTAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

29.61

Weight (kDa)

7.57

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 668
AccII CGCG 2 cut(s) 87, 277
AciI CCGC 1 cut(s) 629
AclWI GGATC 1 cut(s) 185
AcsI RAATTY 2 cut(s) 92, 783
AfaI GTAC 1 cut(s) 777
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 2 cut(s) 151, 572
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 3 cut(s) 164, 345, 592
AluI AGCT 3 cut(s) 164, 345, 592
AlwI GGATC 1 cut(s) 185
AlwNI CAGNNNCTG 2 cut(s) 102, 764
Ama87I CYCGRG 2 cut(s) 642, 668
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 164
ApoI RAATTY 2 cut(s) 92, 783
AseI ATTAAT 1 cut(s) 732
Asp700I GAANNNNTTC 1 cut(s) 518
AvaI CYCGRG 2 cut(s) 642, 668
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 151
BccI CCATC 1 cut(s) 731
BclI TGATCA 1 cut(s) 397
BfaI CTAG 3 cut(s) 119, 215, 779
BfrI CTTAAG 1 cut(s) 509
BglII AGATCT 1 cut(s) 307
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
BmcAI AGTACT 1 cut(s) 777
BmeT110I CYCGRG 2 cut(s) 642, 668
BmsI GCATC 3 cut(s) 437, 557, 656
BpiI GAAGAC 1 cut(s) 189
Bse1I ACTGG 1 cut(s) 107
BseGI GGATG 3 cut(s) 200, 412, 746
BseNI ACTGG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 151
Bsh1236I CGCG 2 cut(s) 87, 277
BshFI GGCC 1 cut(s) 106
BsiHKCI CYCGRG 2 cut(s) 642, 668
BsnI GGCC 1 cut(s) 106
BsoBI CYCGRG 2 cut(s) 642, 668
Bsp143I GATC 5 cut(s) 12, 190, 307, 355, 397
BspACI CCGC 1 cut(s) 629
BspANI GGCC 1 cut(s) 106
BspFNI CGCG 2 cut(s) 87, 277
BspPI GGATC 1 cut(s) 185
BspTI CTTAAG 1 cut(s) 509
BsrI ACTGG 1 cut(s) 107
BssMI GATC 5 cut(s) 12, 190, 307, 355, 397
Bst4CI ACNGT 1 cut(s) 49
BstAFI CTTAAG 1 cut(s) 509
BstDEI CTNAG 1 cut(s) 596
BstF5I GGATG 3 cut(s) 200, 412, 746
BstFNI CGCG 2 cut(s) 87, 277
BstKTI GATC 5 cut(s) 15, 193, 310, 358, 400
BstMBI GATC 5 cut(s) 12, 190, 307, 355, 397
BstMWI GCNNNNNNNGC 2 cut(s) 342, 644
BstUI CGCG 2 cut(s) 87, 277
BstV1I GCAGC 1 cut(s) 151
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 190, 307
BstYI RGATCY 2 cut(s) 190, 307
BsuRI GGCC 1 cut(s) 106
BtsCI GGATG 3 cut(s) 200, 412, 746
BtsIMutI CAGTG 1 cut(s) 103
CaiI CAGNNNCTG 2 cut(s) 102, 764
Csp6I GTAC 1 cut(s) 776
CviAII CATG 1 cut(s) 110
CviJI RGCY 6 cut(s) 82, 106, 164, 174, 345, 592
CviKI_1 RGCY 6 cut(s) 82, 106, 164, 174, 345, 592
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 1 cut(s) 596
DpnI GATC 5 cut(s) 14, 192, 309, 357, 399
DpnII GATC 5 cut(s) 12, 190, 307, 355, 397
Eco88I CYCGRG 2 cut(s) 642, 668
EcoRI GAATTC 1 cut(s) 92
FaeI CATG 1 cut(s) 113
FaiI YATR 6 cut(s) 111, 290, 437, 462, 771, 773
FatI CATG 1 cut(s) 109
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 3 cut(s) 187, 419, 753
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 3 cut(s) 119, 215, 779
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 1 cut(s) 106
Hin1II CATG 1 cut(s) 113
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 4 cut(s) 386, 575, 682, 761
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 6 cut(s) 205, 232, 360, 385, 394, 766
Hpy188III TCNNGA 5 cut(s) 185, 572, 579, 642, 754
Hpy8I GTNNAC 1 cut(s) 261
Hpy99I CGWCG 1 cut(s) 343
HpyAV CCTTC 2 cut(s) 469, 688
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 6 cut(s) 4, 71, 250, 450, 464, 542
HpyF10VI GCNNNNNNNGC 2 cut(s) 342, 644
HpyF3I CTNAG 1 cut(s) 596
Hsp92II CATG 1 cut(s) 113
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 5 cut(s) 12, 190, 307, 355, 397
LmnI GCTCC 1 cut(s) 386
LpnPI CCDG 5 cut(s) 88, 170, 297, 379, 558
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 3 cut(s) 437, 557, 656
MaeI CTAG 3 cut(s) 119, 215, 779
MaeIII GTNAC 1 cut(s) 98
MalI GATC 5 cut(s) 14, 192, 309, 357, 399
MboI GATC 5 cut(s) 12, 190, 307, 355, 397
MfeI CAATTG 2 cut(s) 123, 480
MflI RGATCY 2 cut(s) 190, 307
MluCI AATT 9 cut(s) 92, 123, 146, 220, 245, 331, 480, 495, 783
MlyI GAGTC 1 cut(s) 584
MmeI TCCRAC 2 cut(s) 297, 710
MnlI CCTC 5 cut(s) 117, 226, 343, 664, 677
MroXI GAANNNNTTC 1 cut(s) 518
MseI TTAA 4 cut(s) 272, 510, 609, 732
MslI CAYNNNNRTG 1 cut(s) 405
MspCI CTTAAG 1 cut(s) 509
MunI CAATTG 2 cut(s) 123, 480
MvnI CGCG 2 cut(s) 87, 277
MwoI GCNNNNNNNGC 2 cut(s) 342, 644
NdeII GATC 5 cut(s) 12, 190, 307, 355, 397
NlaIII CATG 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 98
PaeR7I CTCGAG 2 cut(s) 642, 668
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 3 cut(s) 386, 682, 761
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
PshBI ATTAAT 1 cut(s) 732
PspXI VCTCGAGB 1 cut(s) 668
PstNI CAGNNNCTG 2 cut(s) 102, 764
PsuI RGATCY 2 cut(s) 190, 307
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 1 cut(s) 405
SaqAI TTAA 4 cut(s) 272, 510, 609, 732
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 5 cut(s) 12, 190, 307, 355, 397
ScaI AGTACT 1 cut(s) 777
SchI GAGTC 1 cut(s) 584
SetI ASST 7 cut(s) 166, 244, 347, 354, 594, 637, 669
SfaNI GCATC 3 cut(s) 437, 557, 656
Sfr274I CTCGAG 2 cut(s) 642, 668
SlaI CTCGAG 2 cut(s) 642, 668
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 3 cut(s) 509, 642, 668
SmoI CTYRAG 3 cut(s) 509, 642, 668
Sse9I AATT 9 cut(s) 92, 123, 146, 220, 245, 331, 480, 495, 783
SsiI CCGC 1 cut(s) 629
SspMI CTAG 3 cut(s) 119, 215, 779
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 5 cut(s) 341, 529, 578, 643, 669
TasI AATT 9 cut(s) 92, 123, 146, 220, 245, 331, 480, 495, 783
TatI WGTACW 1 cut(s) 775
TfiI GAWTC 3 cut(s) 386, 682, 761
Tru1I TTAA 4 cut(s) 272, 510, 609, 732
Tru9I TTAA 4 cut(s) 272, 510, 609, 732
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 6 cut(s) 159, 189, 378, 423, 671, 723
TspGWI ACGGA 2 cut(s) 183, 303
TspRI CASTG 1 cut(s) 103
Vha464I CTTAAG 1 cut(s) 509
VspI ATTAAT 1 cut(s) 732
XapI RAATTY 2 cut(s) 92, 783
XhoI CTCGAG 2 cut(s) 642, 668
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 3 cut(s) 119, 215, 779
ZrmI AGTACT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.