MD09G1184500.v1.1

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005411
Physical Location & Seq
Reverse (-)
15995072 .. 15998031
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1184500.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGCGGATGCACTTCCTTCTTCGTCAACTCTCTCTTTACTCACTCTACCCAGCTTGCCTCCTCAAAACCCAACTAGACCACTGCAGCATTCTTGTTTCATCTCTACCGCCCGAAAGCGTGATCTTGTTCGATGCAAGGCCAAGAAGAAAATAAGCTTGGCAGACCAAATTCTTGATTACATCGAGGGGGGTCCCAAGTTAAGGAAATGGTATGGGGCACCTGAGCTTCTACCGAAAGATGGATCCGCAGATGAAGAAGATGAGGATGAATTCACAGAAGGAGAAGAACTCAGGGATGCTGTATTGGTAACTGATGGAGATAGTGAGATGGGACAGATGGTAATATTGTCATTGATCGTCAAAAGAGCTAGAATAAAAGCGCTGGTGAAGGATAAGCGGGTAGCACTTGAAGCGTTTGGGACTTATGTTGAGTCAATGGCCGGAGATTCAAAGGACAAGCCGTTCCTAAAGAGAGTTCTACGAGGAGTTTCCACAGTAATATGCCCAAATGAAGGTTTCTTATCTACCGTTGGGAGCTTGAAAGGGGTAAAACATATAATCCTCTTATCTCAGTTGTCCGTCTACAAAGGTGCCAATGGCATTCAAGCTCTCATGAAAAGCAATGCAAGAAAATTGGCTGAGCAAGATGAATCAATAATGGTGGCCTCTGGAATCCCTTACACCGTGATCAGAGCTGGCTCGTTACAAAATTCTCCTGGTGGAACTCAAGGTTTTAGCTTTGAACAGGGAAGTGCAGCAAAAGGAACCCTTAGCAAGGAGGATGCAGCCTTTATTTGTGCAGAAGCAGTTGGTGTAGTCCCTCAGAGAGGATTCATATTTGAGGTGGTGAATGGAGACGAAAAGGTTTCAGATTGGAAAGAGTTTTTGAACGCAATGCTGAAGAAGTCAGAAGAGCAACCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

309

Amino Acids

33.45

Weight (kDa)

6.25

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 111 - 270 8.9e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 217, 590
AccI GTMKAC 1 cut(s) 582
AciI CCGC 4 cut(s) 5, 108, 246, 397
AclWI GGATC 2 cut(s) 237, 250
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 3 cut(s) 168, 269, 709
AcuI CTGAAG 1 cut(s) 920
AfeI AGCGCT 1 cut(s) 381
AfiI CCNNNNNNNGG 5 cut(s) 201, 239, 512, 775, 827
AgsI TTSAA 6 cut(s) 410, 450, 541, 605, 743, 889
AjnI CCWGG 1 cut(s) 715
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AloI GAACNNNNNNTCC 2 cut(s) 446, 478
AluBI AGCT 8 cut(s) 54, 156, 226, 368, 537, 608, 695, 738
AluI AGCT 8 cut(s) 54, 156, 226, 368, 537, 608, 695, 738
Alw26I GTCTC 1 cut(s) 849
AlwI GGATC 2 cut(s) 237, 250
Aor51HI AGCGCT 1 cut(s) 381
AoxI GGCC 3 cut(s) 138, 438, 663
ApeKI GCWGC 3 cut(s) 85, 755, 785
ApoI RAATTY 3 cut(s) 168, 269, 709
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 397, 859
AvaII GGWCC 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 220
BamHI GGATCC 1 cut(s) 242
BanI GGYRCC 2 cut(s) 217, 590
BbvI GCAGC 3 cut(s) 97, 767, 797
BccI CCATC 4 cut(s) 233, 308, 322, 331
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 717
BclI TGATCA 1 cut(s) 687
BcoDI GTCTC 1 cut(s) 849
BfaI CTAG 2 cut(s) 75, 369
BfmI CTRYAG 1 cut(s) 83
BfoI RGCGCY 1 cut(s) 383
BisI GCNGC 3 cut(s) 86, 756, 786
BlpI GCTNAGC 1 cut(s) 639
BlsI GCNGC 3 cut(s) 87, 757, 787
Bme1390I CCNGG 1 cut(s) 717
Bme18I GGWCC 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 6 cut(s) 192, 193, 219, 244, 592, 766
BmrFI CCNGG 1 cut(s) 717
BmsI GCATC 3 cut(s) 122, 286, 772
BplI GAGNNNNNCTC 2 cut(s) 273, 305
Bpu10I CCTNAGC 2 cut(s) 222, 770
Bpu1102I GCTNAGC 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 711
Bsc4I CCNNNNNNNGG 5 cut(s) 201, 239, 512, 775, 827
Bse3DI GCAATG 2 cut(s) 628, 900
BseBI CCWGG 1 cut(s) 717
BseGI GGATG 4 cut(s) 13, 271, 301, 787
BseLI CCNNNNNNNGG 5 cut(s) 201, 239, 512, 775, 827
BseMI GCAATG 2 cut(s) 628, 900
BseMII CTCAG 5 cut(s) 213, 304, 584, 630, 836
BseRI GAGGAG 2 cut(s) 51, 498
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 3 cut(s) 97, 767, 797
BseYI CCCAGC 1 cut(s) 50
BsgI GTGCAG 2 cut(s) 774, 819
BshFI GGCC 3 cut(s) 140, 440, 665
BshNI GGYRCC 2 cut(s) 217, 590
BsiSI CCGG 1 cut(s) 441
BslFI GGGAC 4 cut(s) 177, 345, 433, 803
BslI CCNNNNNNNGG 5 cut(s) 201, 239, 512, 775, 827
BsmAI GTCTC 1 cut(s) 849
BsmBI CGTCTC 1 cut(s) 849
BsmFI GGGAC 4 cut(s) 177, 345, 433, 803
BsmI GAATGC 2 cut(s) 88, 600
BsnI GGCC 3 cut(s) 140, 440, 665
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 4 cut(s) 121, 242, 354, 687
Bsp1720I GCTNAGC 1 cut(s) 639
BspACI CCGC 4 cut(s) 5, 108, 246, 397
BspANI GGCC 3 cut(s) 140, 440, 665
BspCNI CTCAG 5 cut(s) 214, 303, 583, 631, 835
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 6 cut(s) 192, 193, 219, 244, 592, 766
BspMAI CTGCAG 1 cut(s) 87
BspPI GGATC 2 cut(s) 237, 250
BspQI GCTCTTC 1 cut(s) 906
BspT107I GGYRCC 2 cut(s) 217, 590
BsrDI GCAATG 2 cut(s) 628, 900
BssMI GATC 4 cut(s) 121, 242, 354, 687
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 3 cut(s) 496, 529, 685
Bst6I CTCTTC 1 cut(s) 906
BstC8I GCNNGC 2 cut(s) 56, 697
BstDEI CTNAG 7 cut(s) 222, 290, 570, 639, 770, 822, 921
BstENI CCTNNNNNAGG 2 cut(s) 773, 825
BstF5I GGATG 4 cut(s) 13, 271, 301, 787
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 4 cut(s) 124, 245, 357, 690
BstMAI GTCTC 1 cut(s) 849
BstMBI GATC 4 cut(s) 121, 242, 354, 687
BstMWI GCNNNNNNNGC 1 cut(s) 410
BstNI CCWGG 1 cut(s) 717
BstSCI CCNGG 1 cut(s) 715
BstSFI CTRYAG 1 cut(s) 83
BstSLI GKGCMC 1 cut(s) 220
BstV1I GCAGC 3 cut(s) 97, 767, 797
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 3 cut(s) 140, 440, 665
BtsCI GGATG 4 cut(s) 13, 271, 301, 787
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 1 cut(s) 80
Cac8I GCNNGC 2 cut(s) 56, 697
CciI TCATGA 1 cut(s) 612
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 1 cut(s) 613
DdeI CTNAG 7 cut(s) 222, 290, 570, 639, 770, 822, 921
DpnI GATC 4 cut(s) 123, 244, 356, 689
DpnII GATC 4 cut(s) 121, 242, 354, 687
EaeI YGGCCR 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 906
EarI CTCTTC 1 cut(s) 906
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 191
Eco47III AGCGCT 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 920
EcoNI CCTNNNNNAGG 2 cut(s) 773, 825
EcoO109I RGGNCCY 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 715
Esp3I CGTCTC 1 cut(s) 849
FaeI CATG 1 cut(s) 616
FaiI YATR 7 cut(s) 213, 426, 502, 555, 557, 614, 836
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FaqI GGGAC 4 cut(s) 177, 345, 433, 803
FatI CATG 1 cut(s) 612
FauI CCCGC 1 cut(s) 390
FbaI TGATCA 1 cut(s) 687
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 3 cut(s) 86, 756, 786
FokI GGATG 4 cut(s) 20, 278, 308, 794
Fsp4HI GCNGC 3 cut(s) 86, 756, 786
FspBI CTAG 2 cut(s) 75, 369
GlaI GCGC 1 cut(s) 381
GluI GCNGC 3 cut(s) 86, 756, 786
GsaI CCCAGC 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 3 cut(s) 140, 440, 665
HapII CCGG 1 cut(s) 441
HhaI GCGC 1 cut(s) 382
Hin1II CATG 1 cut(s) 616
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 5 cut(s) 431, 446, 650, 672, 831
HpaII CCGG 1 cut(s) 441
HphI GGTGA 2 cut(s) 397, 859
Hpy166II GTNNAC 2 cut(s) 27, 583
Hpy188I TCNGA 4 cut(s) 692, 825, 871, 910
Hpy188III TCNNGA 3 cut(s) 173, 613, 669
Hpy8I GTNNAC 2 cut(s) 27, 583
HpyAV CCTTC 4 cut(s) 27, 272, 382, 506
HpyCH4III ACNGT 3 cut(s) 496, 529, 685
HpyCH4V TGCA 7 cut(s) 11, 85, 135, 626, 755, 785, 800
HpyF10VI GCNNNNNNNGC 1 cut(s) 410
HpyF3I CTNAG 7 cut(s) 222, 290, 570, 639, 770, 822, 921
Hsp92II CATG 1 cut(s) 616
HspAI GCGC 1 cut(s) 380
KflI GGGWCCC 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 687
Kzo9I GATC 4 cut(s) 121, 242, 354, 687
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 3 cut(s) 97, 767, 797
LweI GCATC 3 cut(s) 122, 286, 772
MaeI CTAG 2 cut(s) 75, 369
MaeIII GTNAC 2 cut(s) 307, 702
MalI GATC 4 cut(s) 123, 244, 356, 689
MboI GATC 4 cut(s) 121, 242, 354, 687
MboII GAAGA 7 cut(s) 12, 157, 266, 269, 296, 913, 923
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 4 cut(s) 168, 269, 632, 709
MlyI GAGTC 1 cut(s) 440
MseI TTAA 1 cut(s) 200
MspI CCGG 1 cut(s) 441
MspR9I CCNGG 1 cut(s) 717
Mva1269I GAATGC 2 cut(s) 88, 600
MvaI CCWGG 1 cut(s) 717
MwoI GCNNNNNNNGC 1 cut(s) 410
NdeII GATC 4 cut(s) 121, 242, 354, 687
NlaIII CATG 1 cut(s) 616
NlaIV GGNNCC 6 cut(s) 192, 193, 219, 244, 592, 766
PagI TCATGA 1 cut(s) 612
PciSI GCTCTTC 1 cut(s) 906
PctI GAATGC 2 cut(s) 88, 600
PfeI GAWTC 4 cut(s) 446, 650, 672, 831
PkrI GCNGC 3 cut(s) 87, 757, 787
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
PpuMI RGGWCCY 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 715
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 6 cut(s) 192, 193, 219, 244, 592, 766
PspPI GGNCC 1 cut(s) 191
PspPPI RGGWCCY 1 cut(s) 191
PstI CTGCAG 1 cut(s) 87
PsuI RGATCY 1 cut(s) 242
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 3 cut(s) 86, 756, 786
Sau3AI GATC 4 cut(s) 121, 242, 354, 687
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 1 cut(s) 220
SfaNI GCATC 3 cut(s) 122, 286, 772
SfcI CTRYAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
Sse9I AATT 4 cut(s) 168, 269, 632, 709
SsiI CCGC 4 cut(s) 5, 108, 246, 397
SspI AATATT 1 cut(s) 345
SspMI CTAG 2 cut(s) 75, 369
StyD4I CCNGG 1 cut(s) 715
TaaI ACNGT 3 cut(s) 496, 529, 685
TaqI TCGA 2 cut(s) 130, 183
TasI AATT 4 cut(s) 168, 269, 632, 709
TfiI GAWTC 4 cut(s) 446, 650, 672, 831
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 1 cut(s) 87
TseI GCWGC 3 cut(s) 85, 755, 785
TspDTI ATGAA 7 cut(s) 88, 267, 282, 525, 629, 663, 823
TspGWI ACGGA 1 cut(s) 568
TspRI CASTG 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 191
XagI CCTNNNNNAGG 2 cut(s) 773, 825
XapI RAATTY 3 cut(s) 168, 269, 709
XmiI GTMKAC 1 cut(s) 582
XspI CTAG 2 cut(s) 75, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.