Rh2DG464000

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005411
Physical Location & Seq
Forward (+)
67196333 .. 67199465
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG464000.1

Sequence Viewer

Length: 906 bp
ATGGCGGGTGTACTTCCTTGTTCATCATCAACTCTCTCTCTACTAACTCCACTCAATTTCCATTCTCAAGAGCAATGCAGAGCTTTGTATTCTCAATCTTTAAGCACCCAAAGGTGTGGCCTTTTTGTTTGTGCTGCCAAGAAGAAAGTTAGTTTCACAGACCAAATTCTTGATTACATTGAGGGGGGTCCTAAGTTGAGGAAATGGTATGGGGCACCTGAACTTTTACCTAAGGATGGGTCTGTTGCTGAAGATGAGGACGAATTATCAGAAGGAGATGAAACCAGGGATGCTGTTTTGGTAACCGACGGAGATAGCGAGATGGGGCAGATGGTAATATTGTCATTGATTGTCAAAAGAGCTAGAGTAAAAGCATTGGTGAAGGATAAGCGGGCTGCACTTGAAGCATTTGGAACTTATGTTGAGTCATTCACTGGAGATTCAAGGGACAAGCCATTCCTAAGGAGAGTGCTGAGAGGAGTTTCCACAGTAATATGCCCAAATGAAGGTTTCTTATCTACTGTTGGGAGCTTGAAAGGGGTAAACCATGTCATTCTCTTATCTCAGTTGTCTGTTTACAAAGGTGCCGGTGGCATTCAAGCTCTCATGAAAAGCAATGCAAGAAAATTAGCCGAAGAAGATGAGTCAATACTGATGGCCTCAGGAATCCCTTACACCATCATCAGGGCTGGCTCGTTACAAAATTCTCCAGGTGGAACTCAGGGCTTTAGCTTTGAAAAGGGAACTGCAGCAAAAGGAACTCTTAGCAAAGAGGATGCGGCCTTTATTTGTGTGGAAGCAGTTGATGCAGTCCCCGAGGAAAGATTCATATTTGAGGTGGTGAATGGAGATGACAAGGTTTCAGATTGGAAAGAATGTTTGAATAGACTAATGGAGAAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

301

Amino Acids

32.65

Weight (kDa)

5.36

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 108 - 269 5.6e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 214, 584
AciI CCGC 3 cut(s) 5, 391, 779
AcsI RAATTY 2 cut(s) 165, 703
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 3 cut(s) 236, 506, 684
AgsI TTSAA 6 cut(s) 404, 444, 535, 599, 737, 883
AjnI CCWGG 2 cut(s) 284, 709
AluBI AGCT 5 cut(s) 83, 362, 531, 602, 732
AluI AGCT 5 cut(s) 83, 362, 531, 602, 732
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 3 cut(s) 118, 657, 780
ApeKI GCWGC 3 cut(s) 134, 395, 749
ApoI RAATTY 2 cut(s) 165, 703
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 391, 853
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 1 cut(s) 188
AxyI CCTNAGG 3 cut(s) 231, 461, 661
BaeGI GKGCMC 1 cut(s) 217
BanI GGYRCC 2 cut(s) 214, 584
BbvI GCAGC 3 cut(s) 121, 382, 761
BccI CCATC 5 cut(s) 230, 316, 325, 649, 686
BciT130I CCWGG 2 cut(s) 286, 711
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 747
BisI GCNGC 4 cut(s) 135, 396, 750, 780
BlsI GCNGC 4 cut(s) 136, 397, 751, 781
Bme1390I CCNGG 2 cut(s) 286, 711
Bme18I GGWCC 1 cut(s) 188
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 3 cut(s) 189, 216, 586
BmrFI CCNGG 2 cut(s) 286, 711
BmsI GCATC 3 cut(s) 280, 766, 796
BpmI CTGGAG 2 cut(s) 456, 693
BpuEI CTTGAG 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 285, 816
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 506, 684
Bse118I RCCGGY 1 cut(s) 587
Bse1I ACTGG 1 cut(s) 439
Bse21I CCTNAGG 3 cut(s) 231, 461, 661
Bse3DI GCAATG 2 cut(s) 80, 622
BseBI CCWGG 2 cut(s) 286, 711
BseDI CCNNGG 2 cut(s) 285, 816
BseGI GGATG 3 cut(s) 241, 295, 781
BseLI CCNNNNNNNGG 3 cut(s) 236, 506, 684
BseMI GCAATG 2 cut(s) 80, 622
BseMII CTCAG 4 cut(s) 464, 578, 675, 734
BseNI ACTGG 1 cut(s) 439
BseRI GAGGAG 1 cut(s) 492
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 3 cut(s) 121, 382, 761
BsgI GTGCAG 1 cut(s) 381
BshFI GGCC 3 cut(s) 120, 659, 782
BshNI GGYRCC 2 cut(s) 214, 584
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 1 cut(s) 588
BslFI GGGAC 2 cut(s) 461, 797
BslI CCNNNNNNNGG 3 cut(s) 236, 506, 684
BsmFI GGGAC 2 cut(s) 461, 797
BsmI GAATGC 1 cut(s) 594
BsnI GGCC 3 cut(s) 120, 659, 782
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 217
BspACI CCGC 3 cut(s) 5, 391, 779
BspANI GGCC 3 cut(s) 120, 659, 782
BspCNI CTCAG 4 cut(s) 465, 577, 674, 733
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 3 cut(s) 189, 216, 586
BspMAI CTGCAG 1 cut(s) 751
BspT107I GGYRCC 2 cut(s) 214, 584
BsrDI GCAATG 2 cut(s) 80, 622
BsrFI RCCGGY 1 cut(s) 587
BsrI ACTGG 1 cut(s) 439
BssAI RCCGGY 1 cut(s) 587
BssECI CCNNGG 2 cut(s) 285, 816
Bst2UI CCWGG 2 cut(s) 286, 711
Bst4CI ACNGT 2 cut(s) 490, 523
BstAPI GCANNNNNTGC 1 cut(s) 806
BstC8I GCNNGC 2 cut(s) 393, 691
BstDEI CTNAG 8 cut(s) 192, 231, 461, 473, 564, 661, 720, 764
BstEII GGTNACC 1 cut(s) 301
BstF5I GGATG 3 cut(s) 241, 295, 781
BstMWI GCNNNNNNNGC 2 cut(s) 404, 806
BstNI CCWGG 2 cut(s) 286, 711
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 2 cut(s) 284, 709
BstSFI CTRYAG 1 cut(s) 747
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 3 cut(s) 121, 382, 761
BstXI CCANNNNNNTGG 1 cut(s) 116
Bsu36I CCTNAGG 3 cut(s) 231, 461, 661
BsuRI GGCC 3 cut(s) 120, 659, 782
BtsCI GGATG 3 cut(s) 241, 295, 781
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 2 cut(s) 393, 691
CciI TCATGA 1 cut(s) 606
Cfr10I RCCGGY 1 cut(s) 587
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 11
CviAII CATG 2 cut(s) 548, 607
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 8 cut(s) 192, 231, 461, 473, 564, 661, 720, 764
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 270
Eco81I CCTNAGG 3 cut(s) 231, 461, 661
Eco88I CYCGRG 1 cut(s) 815
Eco91I GGTNACC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 284, 709
FaeI CATG 2 cut(s) 551, 610
FaiI YATR 6 cut(s) 210, 420, 496, 549, 608, 830
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 2 cut(s) 461, 797
FatI CATG 2 cut(s) 547, 606
FauI CCCGC 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 135, 396, 750, 780
FokI GGATG 3 cut(s) 248, 302, 788
Fsp4HI GCNGC 4 cut(s) 135, 396, 750, 780
FspBI CTAG 1 cut(s) 363
GluI GCNGC 4 cut(s) 135, 396, 750, 780
GsuI CTGGAG 2 cut(s) 456, 693
HaeIII GGCC 3 cut(s) 120, 659, 782
HapII CCGG 1 cut(s) 588
Hin1II CATG 2 cut(s) 551, 610
HinfI GANTC 5 cut(s) 425, 440, 644, 666, 825
HpaII CCGG 1 cut(s) 588
HphI GGTGA 2 cut(s) 391, 853
Hpy166II GTNNAC 3 cut(s) 11, 544, 577
Hpy188I TCNGA 2 cut(s) 271, 865
Hpy188III TCNNGA 4 cut(s) 68, 170, 607, 663
Hpy8I GTNNAC 3 cut(s) 11, 544, 577
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 3 cut(s) 266, 376, 500
HpyCH4III ACNGT 2 cut(s) 490, 523
HpyCH4V TGCA 5 cut(s) 78, 398, 620, 749, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 806
HpyF3I CTNAG 8 cut(s) 192, 231, 461, 473, 564, 661, 720, 764
Hsp92II CATG 2 cut(s) 551, 610
LmnI GCTCC 1 cut(s) 528
Lsp1109I GCAGC 3 cut(s) 121, 382, 761
LweI GCATC 3 cut(s) 280, 766, 796
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 301, 696
MboII GAAGA 4 cut(s) 154, 263, 647, 650
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 5 cut(s) 55, 165, 263, 626, 703
MlyI GAGTC 2 cut(s) 434, 653
MnlI CCTC 8 cut(s) 175, 192, 250, 470, 670, 766, 811, 829
MseI TTAA 2 cut(s) 101, 904
MspI CCGG 1 cut(s) 588
MspR9I CCNGG 2 cut(s) 286, 711
Mva1269I GAATGC 1 cut(s) 594
MvaI CCWGG 2 cut(s) 286, 711
MwoI GCNNNNNNNGC 2 cut(s) 404, 806
NlaIII CATG 2 cut(s) 551, 610
NlaIV GGNNCC 3 cut(s) 189, 216, 586
PagI TCATGA 1 cut(s) 606
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 594
PfeI GAWTC 3 cut(s) 440, 666, 825
PkrI GCNGC 4 cut(s) 136, 397, 751, 781
PleI GAGTC 2 cut(s) 433, 652
PpsI GAGTC 2 cut(s) 433, 652
PpuMI RGGWCCY 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 284, 709
PspEI GGTNACC 1 cut(s) 301
PspGI CCWGG 2 cut(s) 284, 709
PspN4I GGNNCC 3 cut(s) 189, 216, 586
PspPI GGNCC 1 cut(s) 188
PspPPI RGGWCCY 1 cut(s) 188
PstI CTGCAG 1 cut(s) 751
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 101, 904
SatI GCNGC 4 cut(s) 135, 396, 750, 780
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 2 cut(s) 434, 653
ScrFI CCNGG 2 cut(s) 286, 711
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 3 cut(s) 280, 766, 796
SfcI CTRYAG 1 cut(s) 747
SinI GGWCC 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 5 cut(s) 55, 165, 263, 626, 703
SsiI CCGC 3 cut(s) 5, 391, 779
SspI AATATT 1 cut(s) 339
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 284, 709
TaaI ACNGT 2 cut(s) 490, 523
TasI AATT 5 cut(s) 55, 165, 263, 626, 703
TatI WGTACW 1 cut(s) 10
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 3 cut(s) 440, 666, 825
Tru1I TTAA 2 cut(s) 101, 904
Tru9I TTAA 2 cut(s) 101, 904
TscAI CASTG 1 cut(s) 439
TseI GCWGC 3 cut(s) 134, 395, 749
TspDTI ATGAA 5 cut(s) 12, 294, 519, 623, 817
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 2 cut(s) 165, 703
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.