Rroxscaffold_2G00100870

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005411
Physical Location & Seq
Reverse (-)
22756645 .. 22760016
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00100870.1

Sequence Viewer

Length: 996 bp
ATGTCGTTTATCCTCCCTTGCAGCATTAGCGGCAACTTGAAAAGTAGAAAACGGTTGGATGTGAGAGACAGTGGGAAGAAAAAACCAATGGCGGGTGTACTTCCTTGTGCATCATCAACTCTCTCTCTACTAACTCCACTCAATTTCCATTCTCAAGAGCAATGCAGAGCTTTGTATTCTCACTCTTTAAGCACCCAAAGGTGTGGCCTTTTTGTTTGTGCTGCCAAGAAGAAAGTTAGTTTCACAGACCAAATTCTTGATTACATTGAGGGGGGTCCTAAGTTGAGGAAATGGTATGGGGCACCTGAACTTTTACCTAAGGATGGGTCTGTTGCTGAAGATGAGGACGAATTATCAGAAGGAGATGAAACCAGGGATGCTGTTTTGGTAACTGACGGAGATAGCGAGATGGGGCAGATGGTAATATTGTCGTTGATTGTCAAAAGAGCTAGAGTAAAAGCATTGGTGAAGGATAAGCGGGCTGCACTTGAAGCATTTGGAACTTATGTTGAGTCATTGACTGGAGATTCAAGGGACAAGCCATTCCTAAGGAGAGCGCTGAGAGGAGTTTCCACAGTAATATGCCCAAATGAAGGTTTCTTATCTACTGTTGGGAGCTTGAAAGGGGTAAACCATGTCATTCTCTTATCTCAGCAGTTGTCTGTTTACAAAGGTGCCGGTGGCATTCAAGCTCTCATGAAAAGCAATGCAAGAAAATTAGCCGAAGAAGATGAGTCAATACTGATGGCCTCGGGAATCCCTTACACCATCATCAGGGCTGGCTCATTACAAAATTCTCCAGGTGGAACTCAGGGCTTTAGCTTTGAAAAGGGAACTGCAGCAAAAGGAACTCTTAGCAAAGAGGATGCGGCCTTTATTTGTGTGGAAGCAGTTGATGCAGTCCCCGAGGAAAGATTCATATTTGAGGTGGTGAATGGAGATGACAAGGTTTCAGATTGGAAAGAATGTTTGAATAGACTAATGGAGAAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

331

Amino Acids

35.95

Weight (kDa)

7.51

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 137 - 299 7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 301, 674
AciI CCGC 4 cut(s) 30, 92, 478, 869
AcsI RAATTY 2 cut(s) 252, 793
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 1 cut(s) 99
AfeI AGCGCT 1 cut(s) 558
AfiI CCNNNNNNNGG 4 cut(s) 92, 323, 593, 774
AgsI TTSAA 7 cut(s) 40, 491, 531, 622, 689, 827, 973
AjnI CCWGG 2 cut(s) 371, 799
AluBI AGCT 5 cut(s) 170, 449, 618, 692, 822
AluI AGCT 5 cut(s) 170, 449, 618, 692, 822
Alw26I GTCTC 1 cut(s) 60
Ama87I CYCGRG 2 cut(s) 751, 905
Aor51HI AGCGCT 1 cut(s) 558
AoxI GGCC 3 cut(s) 205, 747, 870
ApeKI GCWGC 4 cut(s) 21, 221, 482, 839
ApoI RAATTY 2 cut(s) 252, 793
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 478, 943
AvaI CYCGRG 2 cut(s) 751, 905
AvaII GGWCC 1 cut(s) 275
AxyI CCTNAGG 2 cut(s) 318, 548
BaeGI GKGCMC 1 cut(s) 304
BanI GGYRCC 2 cut(s) 301, 674
BbvI GCAGC 4 cut(s) 33, 208, 469, 851
BccI CCATC 5 cut(s) 317, 403, 412, 739, 776
BciT130I CCWGG 2 cut(s) 373, 801
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 1 cut(s) 450
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 560
BisI GCNGC 6 cut(s) 22, 31, 222, 483, 840, 870
BlsI GCNGC 6 cut(s) 23, 32, 223, 484, 841, 871
Bme1390I CCNGG 2 cut(s) 373, 801
Bme18I GGWCC 1 cut(s) 275
BmeT110I CYCGRG 2 cut(s) 751, 905
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 3 cut(s) 276, 303, 676
BmrFI CCNGG 2 cut(s) 373, 801
BmsI GCATC 4 cut(s) 119, 367, 856, 886
BpmI CTGGAG 2 cut(s) 543, 783
BpuEI CTTGAG 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 372, 750, 906
Bsc4I CCNNNNNNNGG 4 cut(s) 92, 323, 593, 774
Bse118I RCCGGY 1 cut(s) 677
Bse1I ACTGG 1 cut(s) 526
Bse21I CCTNAGG 2 cut(s) 318, 548
Bse3DI GCAATG 2 cut(s) 167, 712
BseBI CCWGG 2 cut(s) 373, 801
BseDI CCNNGG 3 cut(s) 372, 750, 906
BseGI GGATG 4 cut(s) 64, 328, 382, 871
BseLI CCNNNNNNNGG 4 cut(s) 92, 323, 593, 774
BseMI GCAATG 2 cut(s) 167, 712
BseMII CTCAG 3 cut(s) 551, 665, 824
BseNI ACTGG 1 cut(s) 526
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 4 cut(s) 33, 208, 469, 851
BsgI GTGCAG 1 cut(s) 468
BshFI GGCC 3 cut(s) 207, 749, 872
BshNI GGYRCC 2 cut(s) 301, 674
BsiHKCI CYCGRG 2 cut(s) 751, 905
BsiSI CCGG 1 cut(s) 678
BslFI GGGAC 2 cut(s) 548, 887
BslI CCNNNNNNNGG 4 cut(s) 92, 323, 593, 774
BsmAI GTCTC 1 cut(s) 60
BsmFI GGGAC 2 cut(s) 548, 887
BsmI GAATGC 1 cut(s) 684
BsnI GGCC 3 cut(s) 207, 749, 872
BsoBI CYCGRG 2 cut(s) 751, 905
Bsp1286I GDGCHC 1 cut(s) 304
BspACI CCGC 4 cut(s) 30, 92, 478, 869
BspANI GGCC 3 cut(s) 207, 749, 872
BspCNI CTCAG 3 cut(s) 552, 664, 823
BspHI TCATGA 1 cut(s) 696
BspLI GGNNCC 3 cut(s) 276, 303, 676
BspMAI CTGCAG 1 cut(s) 841
BspT107I GGYRCC 2 cut(s) 301, 674
BsrDI GCAATG 2 cut(s) 167, 712
BsrFI RCCGGY 1 cut(s) 677
BsrI ACTGG 1 cut(s) 526
BssAI RCCGGY 1 cut(s) 677
BssECI CCNNGG 3 cut(s) 372, 750, 906
Bst2UI CCWGG 2 cut(s) 373, 801
Bst4CI ACNGT 4 cut(s) 54, 71, 577, 610
BstAPI GCANNNNNTGC 1 cut(s) 896
BstC8I GCNNGC 2 cut(s) 480, 781
BstDEI CTNAG 7 cut(s) 279, 318, 548, 560, 651, 810, 854
BstF5I GGATG 4 cut(s) 64, 328, 382, 871
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 1 cut(s) 559
BstMAI GTCTC 1 cut(s) 60
BstMWI GCNNNNNNNGC 4 cut(s) 27, 30, 491, 896
BstNI CCWGG 2 cut(s) 373, 801
BstSCI CCNGG 2 cut(s) 371, 799
BstSFI CTRYAG 1 cut(s) 837
BstSLI GKGCMC 1 cut(s) 304
BstV1I GCAGC 4 cut(s) 33, 208, 469, 851
BstXI CCANNNNNNTGG 1 cut(s) 203
Bsu36I CCTNAGG 2 cut(s) 318, 548
BsuRI GGCC 3 cut(s) 207, 749, 872
BtsCI GGATG 4 cut(s) 64, 328, 382, 871
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 2 cut(s) 480, 781
CciI TCATGA 1 cut(s) 696
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 1 cut(s) 677
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 98
CviAII CATG 2 cut(s) 635, 697
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 7 cut(s) 279, 318, 548, 560, 651, 810, 854
Eco47I GGWCC 1 cut(s) 275
Eco47III AGCGCT 1 cut(s) 558
Eco57I CTGAAG 1 cut(s) 357
Eco81I CCTNAGG 2 cut(s) 318, 548
Eco88I CYCGRG 2 cut(s) 751, 905
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 371, 799
FaeI CATG 2 cut(s) 638, 700
FaiI YATR 6 cut(s) 297, 507, 583, 636, 698, 920
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 2 cut(s) 548, 887
FatI CATG 2 cut(s) 634, 696
FauI CCCGC 2 cut(s) 85, 471
Fnu4HI GCNGC 6 cut(s) 22, 31, 222, 483, 840, 870
FokI GGATG 4 cut(s) 71, 335, 389, 878
Fsp4HI GCNGC 6 cut(s) 22, 31, 222, 483, 840, 870
FspBI CTAG 1 cut(s) 450
GlaI GCGC 1 cut(s) 558
GluI GCNGC 6 cut(s) 22, 31, 222, 483, 840, 870
GsuI CTGGAG 2 cut(s) 543, 783
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 3 cut(s) 207, 749, 872
HapII CCGG 1 cut(s) 678
HhaI GCGC 1 cut(s) 559
Hin1II CATG 2 cut(s) 638, 700
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HinfI GANTC 5 cut(s) 512, 527, 734, 756, 915
HpaII CCGG 1 cut(s) 678
HphI GGTGA 2 cut(s) 478, 943
Hpy166II GTNNAC 3 cut(s) 98, 631, 667
Hpy188I TCNGA 2 cut(s) 358, 955
Hpy188III TCNNGA 4 cut(s) 155, 257, 697, 753
Hpy8I GTNNAC 3 cut(s) 98, 631, 667
HpyAV CCTTC 3 cut(s) 353, 463, 587
HpyCH4III ACNGT 4 cut(s) 54, 71, 577, 610
HpyCH4V TGCA 7 cut(s) 21, 110, 165, 485, 710, 839, 899
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 30, 491, 896
HpyF3I CTNAG 7 cut(s) 279, 318, 548, 560, 651, 810, 854
Hsp92II CATG 2 cut(s) 638, 700
HspAI GCGC 1 cut(s) 557
LmnI GCTCC 1 cut(s) 615
Lsp1109I GCAGC 4 cut(s) 33, 208, 469, 851
LweI GCATC 4 cut(s) 119, 367, 856, 886
MaeI CTAG 1 cut(s) 450
MaeIII GTNAC 1 cut(s) 388
MboII GAAGA 5 cut(s) 88, 241, 350, 737, 740
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 5 cut(s) 142, 252, 350, 716, 793
MlyI GAGTC 2 cut(s) 521, 743
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 9 cut(s) 23, 262, 279, 337, 557, 760, 856, 901, 919
MseI TTAA 2 cut(s) 188, 994
MspI CCGG 1 cut(s) 678
MspR9I CCNGG 2 cut(s) 373, 801
Mva1269I GAATGC 1 cut(s) 684
MvaI CCWGG 2 cut(s) 373, 801
MwoI GCNNNNNNNGC 4 cut(s) 27, 30, 491, 896
NlaIII CATG 2 cut(s) 638, 700
NlaIV GGNNCC 3 cut(s) 276, 303, 676
PagI TCATGA 1 cut(s) 696
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 684
PfeI GAWTC 3 cut(s) 527, 756, 915
PkrI GCNGC 6 cut(s) 23, 32, 223, 484, 841, 871
PleI GAGTC 2 cut(s) 520, 742
PpsI GAGTC 2 cut(s) 520, 742
PpuMI RGGWCCY 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 371, 799
PspGI CCWGG 2 cut(s) 371, 799
PspN4I GGNNCC 3 cut(s) 276, 303, 676
PspPI GGNCC 1 cut(s) 275
PspPPI RGGWCCY 1 cut(s) 275
PstI CTGCAG 1 cut(s) 841
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 188, 994
SatI GCNGC 6 cut(s) 22, 31, 222, 483, 840, 870
Sau96I GGNCC 1 cut(s) 275
SchI GAGTC 2 cut(s) 521, 743
ScrFI CCNGG 2 cut(s) 373, 801
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 4 cut(s) 119, 367, 856, 886
SfcI CTRYAG 1 cut(s) 837
SinI GGWCC 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse9I AATT 5 cut(s) 142, 252, 350, 716, 793
SsiI CCGC 4 cut(s) 30, 92, 478, 869
SspI AATATT 1 cut(s) 426
SspMI CTAG 1 cut(s) 450
StyD4I CCNGG 2 cut(s) 371, 799
TaaI ACNGT 4 cut(s) 54, 71, 577, 610
TasI AATT 5 cut(s) 142, 252, 350, 716, 793
TatI WGTACW 1 cut(s) 97
TauI GCSGC 2 cut(s) 33, 872
TfiI GAWTC 3 cut(s) 527, 756, 915
Tru1I TTAA 2 cut(s) 188, 994
Tru9I TTAA 2 cut(s) 188, 994
TscAI CASTG 1 cut(s) 76
TseI GCWGC 4 cut(s) 21, 221, 482, 839
TspDTI ATGAA 4 cut(s) 381, 606, 713, 907
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 2 cut(s) 252, 793
XspI CTAG 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.