MD09G1192800.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011096
Physical Location & Seq
Forward (+)
17187674 .. 17188618
945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1192800.v1.1.491

Sequence Viewer

Length: 945 bp
ATGAGTAGTAGTAGCCATAGCCATAAACACACTCATCACATTTCCACGACAACAACCACAACACCACCAACCAAAGGCAACGCCAACGCCAACACGCAGGCTTGCGCTGCGTGCAAATACCAGCGCAGAAAGTGCGCCTCCGACTGCATTCTAGCCCCCTACTTTGCCCACGACCGCCAGCGCCAGTTCCTCAACGCTCACAAGCTCTTCGGTGTCAGCAACATCAAGAAAATCATCAAACACCTTGACCAGCCGGACAAGGACGATGCCATGCGCACCATCATCTTCCAGTCTGACGTTCGCGCCAACGACCCTGTTGGCGGTTGCTACAGAATCATTCGTGAGCTGCAGCGCTTGATCGAGTACAACAAGGCCGAGCTCGACATTGTTCTTCACCAGCTAGCCATATGCAGAGCACAGGCACAACAAGTTCATCAACAACAACACCAAAACCAGATTCCAATGCCCGAAATCGGAGATCAAAACGCGTGCGATCCGTTGAAAGTTGTGGACCCTTGGAGCTTGTACGGCAACCCAATAGGGCATTTTTATTACATTCAACAACCTCAGCCTCCTCACCATGCAGTTGCACTGCAACAAGAACAACAACAACAACAAGTGCCTTATGTGCTTGTTCAAAACAATACTAATAGCAACAACAATAGTGACATTACTAATGGGGACCCACATCCATATCCACATCAGCATTTGCATGAAGACTTGAGCATGTGGGCCATGCAGGATTCTGTCTCTTGCTTGGGTATCAAGCAATGTGGTGGTGGGATGAATGGGATTACCAATGTATGTGAGGATTTGAAGCCGTTCGTTGATCAGATTGCTTGTGGTGACGATCATGAGAGAAATAATGGGGAGGTGATCGATATTAATGGTGATCATCAACAAAGGTTCTTAGTGACAAACACAGAATTACTCATGTCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

315

Amino Acids

35.45

Weight (kDa)

6.26

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 34 - 129 1.2e-34 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 275
AccII CGCG 2 cut(s) 303, 488
AciI CCGC 2 cut(s) 175, 321
AclWI GGATC 1 cut(s) 488
AfaI GTAC 2 cut(s) 365, 527
AfeI AGCGCT 1 cut(s) 353
AfiI CCNNNNNNNGG 3 cut(s) 74, 320, 473
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 4 cut(s) 502, 560, 638, 817
AjuI GAANNNNNNNTTGG 2 cut(s) 441, 473
AluBI AGCT 5 cut(s) 205, 346, 379, 400, 522
AluI AGCT 5 cut(s) 205, 346, 379, 400, 522
Alw21I GWGCWC 2 cut(s) 381, 418
Alw26I GTCTC 1 cut(s) 754
AlwI GGATC 1 cut(s) 488
Aor51HI AGCGCT 1 cut(s) 353
AoxI GGCC 2 cut(s) 372, 732
ApeKI GCWGC 3 cut(s) 107, 346, 349
AseI ATTAAT 1 cut(s) 885
Asp700I GAANNNNTTC 1 cut(s) 821
AspLEI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
AspS9I GGNCC 3 cut(s) 511, 682, 732
AsuHPI GGTGA 5 cut(s) 386, 569, 857, 886, 902
AsuNHI GCTAGC 1 cut(s) 400
AvaII GGWCC 2 cut(s) 511, 682
BanII GRGCYC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 2 cut(s) 381, 418
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 3 cut(s) 94, 333, 361
BccI CCATC 1 cut(s) 287
BceAI ACGGC 2 cut(s) 544, 805
BclI TGATCA 2 cut(s) 829, 892
BcoDI GTCTC 1 cut(s) 754
BfaI CTAG 2 cut(s) 152, 401
BfmI CTRYAG 2 cut(s) 328, 347
BfoI RGCGCY 2 cut(s) 184, 355
BisI GCNGC 3 cut(s) 108, 347, 350
BlsI GCNGC 3 cut(s) 109, 348, 351
Bme18I GGWCC 2 cut(s) 511, 682
BmgT120I GGNCC 3 cut(s) 511, 682, 732
BmiI GGNNCC 3 cut(s) 513, 683, 684
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 404
BpiI GAAGAC 1 cut(s) 723
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 742
Bsa29I ATCGAT 1 cut(s) 879
BsaJI CCNNGG 1 cut(s) 515
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 320, 473
Bse1I ACTGG 2 cut(s) 184, 289
Bse3DI GCAATG 1 cut(s) 776
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 1 cut(s) 515
BseGI GGATG 3 cut(s) 688, 789, 938
BseLI CCNNNNNNNGG 3 cut(s) 74, 320, 473
BseMI GCAATG 1 cut(s) 776
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 2 cut(s) 184, 289
BseRI GAGGAG 1 cut(s) 564
BseXI GCAGC 3 cut(s) 94, 333, 361
Bsh1236I CGCG 2 cut(s) 303, 488
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 2 cut(s) 374, 734
BshVI ATCGAT 1 cut(s) 879
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 381, 418
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 3 cut(s) 74, 320, 473
BsmAI GTCTC 1 cut(s) 754
BsmFI GGGAC 1 cut(s) 695
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 2 cut(s) 374, 734
Bsp1286I GDGCHC 2 cut(s) 381, 418
Bsp143I GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
BspACI CCGC 2 cut(s) 175, 321
BspANI GGCC 2 cut(s) 374, 734
BspCNI CTCAG 1 cut(s) 580
BspDI ATCGAT 1 cut(s) 879
BspFNI CGCG 2 cut(s) 303, 488
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 3 cut(s) 513, 683, 684
BspMAI CTGCAG 1 cut(s) 351
BspOI GCTAGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 488
BspQI GCTCTTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 776
BsrI ACTGG 2 cut(s) 184, 289
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
BssT1I CCWWGG 1 cut(s) 515
Bst6I CTCTTC 1 cut(s) 212
BstAPI GCANNNNNTGC 1 cut(s) 132
BstC8I GCNNGC 6 cut(s) 99, 103, 112, 179, 402, 490
BstDEI CTNAG 2 cut(s) 567, 910
BstF5I GGATG 3 cut(s) 688, 789, 938
BstFNI CGCG 2 cut(s) 303, 488
BstH2I RGCGCY 2 cut(s) 184, 355
BstHHI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
BstKTI GATC 7 cut(s) 360, 481, 496, 832, 853, 879, 895
BstMAI GTCTC 1 cut(s) 754
BstMBI GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 5 cut(s) 107, 111, 132, 528, 628
BstNSI RCATGY 1 cut(s) 730
BstSFI CTRYAG 2 cut(s) 328, 347
BstUI CGCG 2 cut(s) 303, 488
BstV1I GCAGC 3 cut(s) 94, 333, 361
BstV2I GAAGAC 1 cut(s) 723
Bsu15I ATCGAT 1 cut(s) 879
BsuRI GGCC 2 cut(s) 374, 734
BsuTUI ATCGAT 1 cut(s) 879
BtsCI GGATG 3 cut(s) 688, 789, 938
BtsI GCAGTG 1 cut(s) 590
BtsIMutI CAGTG 1 cut(s) 590
Cac8I GCNNGC 6 cut(s) 99, 103, 112, 179, 402, 490
CciI TCATGA 1 cut(s) 853
CfoI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
Cfr13I GGNCC 3 cut(s) 511, 682, 732
ClaI ATCGAT 1 cut(s) 879
Csp6I GTAC 2 cut(s) 364, 526
CspCI CAANNNNNGTGG 2 cut(s) 754, 789
CviAII CATG 7 cut(s) 271, 581, 713, 727, 736, 854, 934
CviQI GTAC 2 cut(s) 364, 526
DdeI CTNAG 2 cut(s) 567, 910
DpnI GATC 7 cut(s) 359, 480, 495, 831, 852, 878, 894
DpnII GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 515
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 2 cut(s) 511, 682
Eco47III AGCGCT 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 379
EcoICRI GAGCTC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 515
FaeI CATG 7 cut(s) 274, 584, 716, 730, 739, 857, 937
FaqI GGGAC 1 cut(s) 695
FatI CATG 7 cut(s) 270, 580, 712, 726, 735, 853, 933
FauNDI CATATG 1 cut(s) 407
FbaI TGATCA 2 cut(s) 829, 892
Fnu4HI GCNGC 3 cut(s) 108, 347, 350
FokI GGATG 3 cut(s) 675, 796, 925
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 3 cut(s) 108, 347, 350
FspAI RTGCGCAY 1 cut(s) 275
FspBI CTAG 2 cut(s) 152, 401
FspI TGCGCA 1 cut(s) 275
GlaI GCGC 7 cut(s) 106, 125, 136, 182, 275, 304, 353
GluI GCNGC 3 cut(s) 108, 347, 350
HaeII RGCGCY 2 cut(s) 184, 355
HaeIII GGCC 2 cut(s) 374, 734
HapII CCGG 1 cut(s) 254
HhaI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
Hin1II CATG 7 cut(s) 274, 584, 716, 730, 739, 857, 937
Hin6I GCGC 7 cut(s) 105, 124, 135, 181, 274, 303, 352
HinP1I GCGC 7 cut(s) 105, 124, 135, 181, 274, 303, 352
HinfI GANTC 3 cut(s) 333, 457, 743
HpaII CCGG 1 cut(s) 254
HphI GGTGA 5 cut(s) 386, 569, 857, 886, 902
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 4 cut(s) 142, 295, 476, 834
Hpy188III TCNNGA 3 cut(s) 226, 341, 854
Hpy8I GTNNAC 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 9 cut(s) 114, 147, 349, 411, 584, 590, 595, 712, 739
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 111, 132, 528, 628
HpyF3I CTNAG 2 cut(s) 567, 910
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 7 cut(s) 274, 584, 716, 730, 739, 857, 937
HspAI GCGC 7 cut(s) 105, 124, 135, 181, 274, 303, 352
KflI GGGWCCC 1 cut(s) 682
Ksp22I TGATCA 2 cut(s) 829, 892
Kzo9I GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 1 cut(s) 519
Lsp1109I GCAGC 3 cut(s) 94, 333, 361
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 152, 401
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 3 cut(s) 665, 845, 913
MalI GATC 7 cut(s) 359, 480, 495, 831, 852, 878, 894
MboI GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
MboII GAAGA 4 cut(s) 199, 277, 383, 728
MhlI GDGCHC 2 cut(s) 381, 418
MluCI AATT 1 cut(s) 926
MluI ACGCGT 1 cut(s) 486
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 7 cut(s) 148, 200, 576, 582, 585, 802, 865
MroXI GAANNNNTTC 1 cut(s) 821
MseI TTAA 1 cut(s) 885
MslI CAYNNNNRTG 1 cut(s) 711
MspI CCGG 1 cut(s) 254
Mva1269I GAATGC 1 cut(s) 147
MvnI CGCG 2 cut(s) 303, 488
MwoI GCNNNNNNNGC 5 cut(s) 107, 111, 132, 528, 628
NdeI CATATG 1 cut(s) 407
NdeII GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 7 cut(s) 274, 584, 716, 730, 739, 857, 937
NlaIV GGNNCC 3 cut(s) 513, 683, 684
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 3 cut(s) 665, 845, 913
NsbI TGCGCA 1 cut(s) 275
NspI RCATGY 1 cut(s) 730
PagI TCATGA 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 212
PctI GAATGC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 3 cut(s) 333, 457, 743
PkrI GCNGC 3 cut(s) 109, 348, 351
PpuMI RGGWCCY 1 cut(s) 682
PshBI ATTAAT 1 cut(s) 885
Psp124BI GAGCTC 1 cut(s) 381
Psp5II RGGWCCY 1 cut(s) 682
PspN4I GGNNCC 3 cut(s) 513, 683, 684
PspPI GGNCC 3 cut(s) 511, 682, 732
PspPPI RGGWCCY 1 cut(s) 682
PstI CTGCAG 1 cut(s) 351
RsaI GTAC 2 cut(s) 365, 527
RsaNI GTAC 2 cut(s) 364, 526
RseI CAYNNNNRTG 1 cut(s) 711
SacI GAGCTC 1 cut(s) 381
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 885
SatI GCNGC 3 cut(s) 108, 347, 350
Sau3AI GATC 7 cut(s) 357, 478, 493, 829, 850, 876, 892
Sau96I GGNCC 3 cut(s) 511, 682, 732
SduI GDGCHC 2 cut(s) 381, 418
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 328, 347
SinI GGWCC 2 cut(s) 511, 682
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 721
SmoI CTYRAG 1 cut(s) 721
Sse9I AATT 1 cut(s) 926
SsiI CCGC 2 cut(s) 175, 321
SspMI CTAG 2 cut(s) 152, 401
SstI GAGCTC 1 cut(s) 381
StyI CCWWGG 1 cut(s) 515
TaiI ACGT 1 cut(s) 300
TaqI TCGA 3 cut(s) 360, 381, 879
TasI AATT 1 cut(s) 926
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 3 cut(s) 333, 457, 743
Tru1I TTAA 1 cut(s) 885
Tru9I TTAA 1 cut(s) 885
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 3 cut(s) 665, 845, 913
TseI GCWGC 3 cut(s) 107, 346, 349
Tsp45I GTSAC 3 cut(s) 665, 845, 913
TspDTI ATGAA 3 cut(s) 422, 729, 800
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 1 cut(s) 597
VpaK11BI GGWCC 2 cut(s) 511, 682
VspI ATTAAT 1 cut(s) 885
XceI RCATGY 1 cut(s) 730
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 2 cut(s) 152, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.