pycom09g10930

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0011096
Physical Location & Seq
Forward (+)
9028632 .. 9029588
957 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10930.1

Sequence Viewer

Length: 957 bp
ATGAGTAGTAGTAGCCATAGCCATAAACACACTCATCACATTTCCACGACAACAACCAAAACACCACCAACCAAAGGCAACGCCAACGCCAACACGCAGGCTTGCGCTGCGTGCAAATACCAGCGCAGAAAGTGCGCCTCTGACTGCATTCTAGCCCCCTACTTCCCCCACGACCGCCAGCGCCAGTTCCTTAACGCTCACAAGCTCTTCGGTGTCAGCAACATCAAGAAAATTATCAAACACCTTGACCAGCCGGACAAGGACGATGCCATGCGCACCATCATCTTCCAGTCTGACGTTCGCGCCAACGACCCTGTTGGCGGTTGCTACAGAATCATTTGCGAGCTGCAGCGCTTGATCGAGTACAACAAGGCCGAGCTTGACATTGTTCTTCACCAGCTAGCCATATGCAGAGCACAAGCACAAGAAGTTTATCAACAACAACATCAAAACCCAATTCAAATGCCGGAAATCGAAGATCAAAACGCGTGCGATCCGTCAATTAAAGTGGACCCTTGGAGCTTGTACGGCAACCCAATGGGGCATTTTTATTACATTCAACAACCTCAGCCTCCTCACCATGCAGTTGCACTGCAACAAGAACAACCGCAACAACAACAACAACAACGAGTGCCTTATGTGTTTGTTCAAAACAATACTAATAGCAACAACAATAGTGACAGTACCAATGGGGACCCACATCCATATCCACATCAACATTTGTATGAAGACTTGAGCATGTGGGCCATGCAGGATTCTGTGTCTTGCTTGGATATCAAGCAATGTGGTGGTGGGATGAATGGGATTACCAACGTATGTGAGGATTTGAAGCCGTTCGTTGATCAGATTGCTTGTGGTGACGATCATGAGAGAAATAATGGGGAGGTGATCGATATTAATGGCGATCATCAACAAAGGTTATTAGTGACAAACACAGAATTACTCATGTCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

319

Amino Acids

36.12

Weight (kDa)

6.02

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 34 - 129 1.3e-35 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 275
AccII CGCG 2 cut(s) 303, 488
AciI CCGC 3 cut(s) 175, 321, 608
AclWI GGATC 1 cut(s) 488
AfaI GTAC 3 cut(s) 365, 527, 685
AfeI AGCGCT 1 cut(s) 353
AfiI CCNNNNNNNGG 2 cut(s) 74, 320
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 4 cut(s) 461, 560, 650, 829
AluBI AGCT 5 cut(s) 205, 346, 379, 400, 522
AluI AGCT 5 cut(s) 205, 346, 379, 400, 522
Alw21I GWGCWC 1 cut(s) 418
AlwI GGATC 1 cut(s) 488
Aor51HI AGCGCT 1 cut(s) 353
AoxI GGCC 2 cut(s) 372, 744
ApeKI GCWGC 3 cut(s) 107, 346, 349
AseI ATTAAT 1 cut(s) 897
Asp700I GAANNNNTTC 1 cut(s) 833
AspLEI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
AspS9I GGNCC 3 cut(s) 511, 694, 744
AsuHPI GGTGA 4 cut(s) 386, 569, 869, 898
AsuNHI GCTAGC 1 cut(s) 400
AvaII GGWCC 2 cut(s) 511, 694
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 418
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 3 cut(s) 94, 333, 361
BccI CCATC 1 cut(s) 287
BceAI ACGGC 2 cut(s) 544, 817
BclI TGATCA 1 cut(s) 841
BfaI CTAG 2 cut(s) 152, 401
BfmI CTRYAG 2 cut(s) 328, 347
BfoI RGCGCY 2 cut(s) 184, 355
BisI GCNGC 3 cut(s) 108, 347, 350
BlsI GCNGC 3 cut(s) 109, 348, 351
Bme18I GGWCC 2 cut(s) 511, 694
BmgT120I GGNCC 3 cut(s) 511, 694, 744
BmiI GGNNCC 3 cut(s) 513, 695, 696
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 404
BpiI GAAGAC 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 754
Bsa29I ATCGAT 1 cut(s) 891
BsaJI CCNNGG 1 cut(s) 515
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 320
Bse1I ACTGG 2 cut(s) 184, 289
Bse3DI GCAATG 1 cut(s) 788
BseCI ATCGAT 1 cut(s) 891
BseDI CCNNGG 1 cut(s) 515
BseGI GGATG 3 cut(s) 700, 801, 950
BseLI CCNNNNNNNGG 2 cut(s) 74, 320
BseMI GCAATG 1 cut(s) 788
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 2 cut(s) 184, 289
BseRI GAGGAG 1 cut(s) 564
BseXI GCAGC 3 cut(s) 94, 333, 361
Bsh1236I CGCG 2 cut(s) 303, 488
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 2 cut(s) 374, 746
BshVI ATCGAT 1 cut(s) 891
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 2 cut(s) 254, 467
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 2 cut(s) 74, 320
BsmFI GGGAC 1 cut(s) 707
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 2 cut(s) 374, 746
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
BspACI CCGC 3 cut(s) 175, 321, 608
BspANI GGCC 2 cut(s) 374, 746
BspCNI CTCAG 1 cut(s) 580
BspDI ATCGAT 1 cut(s) 891
BspFNI CGCG 2 cut(s) 303, 488
BspHI TCATGA 1 cut(s) 865
BspLI GGNNCC 3 cut(s) 513, 695, 696
BspMAI CTGCAG 1 cut(s) 351
BspOI GCTAGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 488
BspQI GCTCTTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 788
BsrI ACTGG 2 cut(s) 184, 289
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
BssT1I CCWWGG 1 cut(s) 515
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 1 cut(s) 212
BstAPI GCANNNNNTGC 1 cut(s) 132
BstC8I GCNNGC 7 cut(s) 99, 103, 112, 179, 344, 402, 490
BstDEI CTNAG 1 cut(s) 567
BstF5I GGATG 3 cut(s) 700, 801, 950
BstFNI CGCG 2 cut(s) 303, 488
BstH2I RGCGCY 2 cut(s) 184, 355
BstHHI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
BstKTI GATC 7 cut(s) 360, 481, 496, 844, 865, 891, 907
BstMBI GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 4 cut(s) 107, 111, 132, 528
BstNSI RCATGY 1 cut(s) 742
BstSFI CTRYAG 2 cut(s) 328, 347
BstUI CGCG 2 cut(s) 303, 488
BstV1I GCAGC 3 cut(s) 94, 333, 361
BstV2I GAAGAC 1 cut(s) 735
Bsu15I ATCGAT 1 cut(s) 891
BsuRI GGCC 2 cut(s) 374, 746
BsuTUI ATCGAT 1 cut(s) 891
BtsCI GGATG 3 cut(s) 700, 801, 950
BtsI GCAGTG 1 cut(s) 590
BtsIMutI CAGTG 1 cut(s) 590
Cac8I GCNNGC 7 cut(s) 99, 103, 112, 179, 344, 402, 490
CciI TCATGA 1 cut(s) 865
CfoI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
Cfr13I GGNCC 3 cut(s) 511, 694, 744
ClaI ATCGAT 1 cut(s) 891
Csp6I GTAC 3 cut(s) 364, 526, 684
CspCI CAANNNNNGTGG 4 cut(s) 489, 524, 766, 801
CviAII CATG 6 cut(s) 271, 581, 739, 748, 866, 946
CviQI GTAC 3 cut(s) 364, 526, 684
DdeI CTNAG 1 cut(s) 567
DpnI GATC 7 cut(s) 359, 480, 495, 843, 864, 890, 906
DpnII GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 515
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 2 cut(s) 511, 694
Eco47III AGCGCT 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 694
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 515
FaeI CATG 6 cut(s) 274, 584, 742, 751, 869, 949
FaqI GGGAC 1 cut(s) 707
FatI CATG 6 cut(s) 270, 580, 738, 747, 865, 945
FauNDI CATATG 1 cut(s) 407
FbaI TGATCA 1 cut(s) 841
Fnu4HI GCNGC 3 cut(s) 108, 347, 350
FokI GGATG 3 cut(s) 687, 808, 937
Fsp4HI GCNGC 3 cut(s) 108, 347, 350
FspAI RTGCGCAY 1 cut(s) 275
FspBI CTAG 2 cut(s) 152, 401
FspI TGCGCA 1 cut(s) 275
GlaI GCGC 7 cut(s) 106, 125, 136, 182, 275, 304, 353
GluI GCNGC 3 cut(s) 108, 347, 350
HaeII RGCGCY 2 cut(s) 184, 355
HaeIII GGCC 2 cut(s) 374, 746
HapII CCGG 2 cut(s) 254, 467
HhaI GCGC 7 cut(s) 107, 126, 137, 183, 276, 305, 354
Hin1II CATG 6 cut(s) 274, 584, 742, 751, 869, 949
Hin6I GCGC 7 cut(s) 105, 124, 135, 181, 274, 303, 352
HinP1I GCGC 7 cut(s) 105, 124, 135, 181, 274, 303, 352
HinfI GANTC 2 cut(s) 333, 755
HpaII CCGG 2 cut(s) 254, 467
HphI GGTGA 4 cut(s) 386, 569, 869, 898
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 3 cut(s) 142, 295, 846
Hpy188III TCNNGA 2 cut(s) 226, 866
Hpy8I GTNNAC 1 cut(s) 511
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 2 cut(s) 297, 813
HpyCH4V TGCA 8 cut(s) 114, 147, 349, 411, 584, 590, 595, 751
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 111, 132, 528
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 2 cut(s) 297, 813
Hsp92II CATG 6 cut(s) 274, 584, 742, 751, 869, 949
HspAI GCGC 7 cut(s) 105, 124, 135, 181, 274, 303, 352
KflI GGGWCCC 1 cut(s) 694
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 1 cut(s) 519
Lsp1109I GCAGC 3 cut(s) 94, 333, 361
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 152, 401
MaeII ACGT 2 cut(s) 297, 813
MaeIII GTNAC 3 cut(s) 677, 857, 925
MalI GATC 7 cut(s) 359, 480, 495, 843, 864, 890, 906
MboI GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
MboII GAAGA 5 cut(s) 199, 277, 383, 488, 740
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 4 cut(s) 231, 456, 501, 938
MluI ACGCGT 1 cut(s) 486
MnlI CCTC 6 cut(s) 148, 576, 582, 585, 814, 877
MroXI GAANNNNTTC 1 cut(s) 833
MseI TTAA 3 cut(s) 192, 504, 897
MslI CAYNNNNRTG 1 cut(s) 723
MspI CCGG 2 cut(s) 254, 467
Mva1269I GAATGC 1 cut(s) 147
MvnI CGCG 2 cut(s) 303, 488
MwoI GCNNNNNNNGC 4 cut(s) 107, 111, 132, 528
NdeI CATATG 1 cut(s) 407
NdeII GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 6 cut(s) 274, 584, 742, 751, 869, 949
NlaIV GGNNCC 3 cut(s) 513, 695, 696
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 3 cut(s) 677, 857, 925
NsbI TGCGCA 1 cut(s) 275
NspI RCATGY 1 cut(s) 742
PagI TCATGA 1 cut(s) 865
PciSI GCTCTTC 1 cut(s) 212
PctI GAATGC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 2 cut(s) 333, 755
PkrI GCNGC 3 cut(s) 109, 348, 351
PpuMI RGGWCCY 1 cut(s) 694
PshBI ATTAAT 1 cut(s) 897
Psp5II RGGWCCY 1 cut(s) 694
PspN4I GGNNCC 3 cut(s) 513, 695, 696
PspPI GGNCC 3 cut(s) 511, 694, 744
PspPPI RGGWCCY 1 cut(s) 694
PstI CTGCAG 1 cut(s) 351
RsaI GTAC 3 cut(s) 365, 527, 685
RsaNI GTAC 3 cut(s) 364, 526, 684
RseI CAYNNNNRTG 1 cut(s) 723
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 3 cut(s) 192, 504, 897
SatI GCNGC 3 cut(s) 108, 347, 350
Sau3AI GATC 7 cut(s) 357, 478, 493, 841, 862, 888, 904
Sau96I GGNCC 3 cut(s) 511, 694, 744
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 2 cut(s) 328, 347
SinI GGWCC 2 cut(s) 511, 694
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 1 cut(s) 733
SmoI CTYRAG 1 cut(s) 733
Sse9I AATT 4 cut(s) 231, 456, 501, 938
SsiI CCGC 3 cut(s) 175, 321, 608
SspMI CTAG 2 cut(s) 152, 401
StyI CCWWGG 1 cut(s) 515
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 2 cut(s) 300, 816
TaqI TCGA 3 cut(s) 360, 474, 891
TasI AATT 4 cut(s) 231, 456, 501, 938
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 2 cut(s) 333, 755
Tru1I TTAA 3 cut(s) 192, 504, 897
Tru9I TTAA 3 cut(s) 192, 504, 897
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 3 cut(s) 677, 857, 925
TseI GCWGC 3 cut(s) 107, 346, 349
Tsp45I GTSAC 3 cut(s) 677, 857, 925
TspDTI ATGAA 2 cut(s) 741, 812
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 1 cut(s) 597
VpaK11BI GGWCC 2 cut(s) 511, 694
VspI ATTAAT 1 cut(s) 897
XceI RCATGY 1 cut(s) 742
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 2 cut(s) 152, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.