MD09G1196000.v1.1

Occludin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005746
Physical Location & Seq
Forward (+)
17817058 .. 17827031
9974 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1196000.v1.1.491

Sequence Viewer

Length: 3690 bp
ATGTACTCATCCAAGTCCGGCCGAGTTGGCGGCGGCGGCGGCTCAGGCCGCGGACCCGGAGCCGGCAAGCTTGGCCGACCCTCCTTCCCTCCACCCCACCGCTCCTCCGCCCACACCACCAGCCGCCTCTCCCTCGGCGGCGGCTCCAATCCGAGAGGCCGCAACTCAGGCCCCCCCACGGCATCAGCCGCCGCCCCGCCGGCCGTGGAAGAGCAGTTCAGTTTAGTCCCCGGCCGCAACCCACTTGCTTTCTCCGTGATCATTCGTATGGCGCCGGACTTGGTCGAAGAAATCAAGCGCGTCGAGGACCAAGGTGGGGCGCCCCGCATCAAGTTTGGTCCGTTGCCTAATAACCCAATGGGCAATGTTATTGATCTGGGTGGGAAGGAATTTACATTTACATGGGACAAGGAATTGGGTGACCTCTGTGACATTTATGAAGAGCGTGAAAGTGGTGAAGGCGGAAATCGTTTGCTAGTTGAATCAGGATGTGCATGGCGCAAAATTAATGTGCAGCGTATCTTAGATGAGTCAACCAAGAACCGTGTTAAACAGTGGTCAGAGGAAGCTGAGCGCAAGAATAAATCCCGCAAAGCCATTGTGCTAGAACAATGTAAAGCTCCAACAAATCAGTTAAAGCAATATGATACTACAGCAGCTAATCAACCATGGAGGAATTTCAAACATAAAATAGAGCCTCCATTTAAGAAGCAGAAAGTTGAACTGCCTCAAGCTCCCCCTAAAACTACATTTAAAACTGGAACGTCATCAGCAACTACTGCAAAGGGCAGGTACTCATCGTCACCTCTTCCAACTCTACCTGAGCAATCTGGGGCTCTATCATCTCCATTAAAAACTGGAAATAGTTTGAAGAGTCATGCAAGTGCAGAAGATGTTATACCCTTTCAAGTGACTAGAAAAGATAAGCCTGCTCCGAGCTCTGATAAAGAAATCCCAATCAGGAACAGTACTGCACCACGACAAACAGTGGGCAAAGACAATAGTGGAGCTAACCCTACAGATTTGCAGAGTATGTTGATTGTTTTGCTCACGGAGAATTCCAAGGGGATGAGAATAAAGGATTTAGAGAAAGCTATTGCAGATTCATATCCAAACTCTATAAAGAAAATTGAGCCTATTATTAAGAAGATTGCTAATTATCAAGCTCCAGGGAGATATGTTTTGAAATCAGGAGTGGAGTTAGAAAGCTCAAAGAAACCTTTGTCTGAAAGTGGAAGTTCTCCCGAGGAGAAACTTCACCAGGAACCTGCTCCTGATGGTAATCGTGATCAAATGCCTGTTCAAGCACCACACTTTGAGGAGAAAGTTTCTCCTAATGGGTTGGAGGAGCAGGTTCACGAACAATTGAATGCTACAGTTGGTCAGGATTCAAGTGTATTGATTAAAATTGATAACCAACAGCATTCCCTTGATTTGTTTGGTGAAAAAAGGGGCTCCGACAATAATGAAGCGCTAGTAGGAAGCTCTAGCGATACTGGGAGTGACAGTGACAGTGATAGTGACACTAGCGATACCGGAAGTGACAGTGGCAGTCATAGCAGAAGCAAAAGTAAAAGTCCAGCACGAAATGGGAGTGGGAGTAGTAGTGATAGTGAAAGTGATGCATCTTCTAATAGCAAGGAAGGTTTTGATGAGGATGTGGATATTATGGCAAGTGACGATGACAAAGAACTGAAGCATCAATTGCAAGACTCTGAACCAGGGTTTTCTACTTTGCCCGTCCCATGGAGAACTCTTGATGGCAAAGCTGTGCAGAGTGGGAATGATGAGAAGCAAGATGATCATGAAGTTGACGCAGTTGAGATTGAAAAACAGTTGCTTGATGCCGATCAGGTTACTGAATTGGCTCCAGTTAGTAACTCTAATCATAATAAAAGTGAACCTCGAGAAGAAACAAAACCCTTTTCACCTGAGCATGGTGAGCTGCAAGACCACCAAAGTTTGGTAGATCCTTTGTATGGAGGGGAGAGTACAGCCAAGGATGACTTTAGGTATGAGCAGTCTGACAGCTCTGAGAGGATATCTAAAGGTACATCCAAAATGGCATCTGGGATAAAGCACTCTGATGAGAAATCTGAACGCAAAAATAGATTAAAAACAGACGTATCACAGCAACCTTCTCACAGTTTGTCTCCTGATAGACTTTTTGAAGGCTCTCATAAGGACCCTGTCTCTCAAACGATTAGTAGAGATGATAGGGATGGGAATATTGAATCTGGATCCCTAAAGGCTTGCAACCAAGCATTTTCTGGCAGATATACTTCAGAGTTTAAACAATCAAGTCGGAAGTCTTTTGATCATAATTTACAGACAAAAGTTCCTGATTCAGCAGAAAGACCAGATAGATATGCTGATAACTTAGGGCATGGCGGGAAGTACTCTGAAAAGAGCTCTCAAATGCATGAAGGTTTCCTCCTGCAAAAAGATAAACTTCATAGAGATACTCAAAATGAGGATGGTTATGCTAATGAGAGAAAGGTTCCAAGAAATTCGAAGGAAGGTGGTACCCGAGGCAAACAGTCAGTGCCCTTGGATCCTCATTACCAGAAAGACGGTGATGTGGCTGGAAAGATGAAGGGGAATGCACAAGTTTCCAGCCTGTTACTGGGGAGTTCACCAAAGGATAACAGCAGAAATGGTGCAGACACATCCCCTGTTGTTCATGGCAGAGGCAGTAAACTCCAAAGAGAGTTTTCAGACTTGGAGTTGGGCGAACTTCGTGAGCCCTTGCTTGAGGAAACGACAGTTAAGAAGCAATTTGAGAGGAAAAGTTCTTTTAAGCAGTCAGATAACAAAACAAGCACTTCAGAGAACAGGGTTTCTGAATTCAGTAAAGTGCAACCTGCAGGAAAGGCAAATTTTGATTTGGGAAGGCCATCCCCTCCAGATAAGCTTCCAAGTAATCAGGAAGGCTTGAATAAAAAGAGGAACTATGAAGATCGCATTGAGGATTTAACAAGGTCGCAACAGAGAGCTGTGCAGTCTCAGTCGCAACACCCATCACGAGTAGATCATCCTGATGTGGGGCGTTCGTTTAGCAAAACAGTGGATCTTACTAGTAAATCTCGACAGAATGAAGCTGGAGGCAGACAAGCAATTGGTGTGGCTGGACATGGAGAAAGCAATAAGAAAGCAACCTCAAGTGCTCCTCAGCAGCTTGACTCAAAGCGAAGGCTAGTTTCCCACCCCATACAAGAAAGTAAAAGAGATGCATTCAATATAATGGTGGATTCAACTGATGTACCAAAGAAATCAATGGTGGCAGAGGGCATTGACAGTGATAGAAAAAAGAGGGATTCTTCTTCAGATGAGAATAGTTGTTCTTATTCTAAGTATGAGAAAAATAAGCCCGAGTTCAAGGGACCAATAAAAGATTTCTCTCAGTACAAAGAGTATGTGCAAGAATATCGTGATAAGTATGATAGCTATTGCTCGTTGAACAAGACCCTAGAGAGTTACAGAAATGAGTTTGAAAAGCTAGGAAAGGACCTTGATTATGCTACTAAAGGCACAGAGAAATATGAAAACATCCTGAGGCAGCTGAAGGATTCATATCGTCAATGTGGAACGAGACATAAGAGGCTGAAAAGAATATTTATGGTACTTCATGAAGAATTGAAGCACCTTAAGCAAAGGATTAAGGACTTTGCAGTCTCATACATGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Occludin_ELL

Protein Analysis

1230

Amino Acids

135.91

Weight (kDa)

7.4

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Occludin_ELL PF07303 1128 - 1224 4.5e-21 Occludin homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3671
Acc36I ACCTGC 4 cut(s) 780, 1276, 1342, 2872
Acc65I GGTACC 1 cut(s) 2525
AccB1I GGYRCC 3 cut(s) 271, 319, 2525
AccB7I CCANNNNNTGG 1 cut(s) 1963
AccBSI CCGCTC 1 cut(s) 102
AccII CGCG 2 cut(s) 51, 300
AclWI GGATC 6 cut(s) 1964, 2235, 2248, 2549, 2562, 3078
AcoI YGGCCR 4 cut(s) 19, 73, 201, 232
AcsI RAATTY 6 cut(s) 389, 676, 1057, 2509, 2846, 2878
AcuI CTGAAG 5 cut(s) 1715, 2268, 2811, 3309, 3584
AcyI GRCGYC 2 cut(s) 272, 320
AfeI AGCGCT 1 cut(s) 1473
AfiI CCNNNNNNNGG 9 cut(s) 62, 178, 316, 1746, 1937, 1963, 1979, 2626, 3044
AflII CTTAAG 1 cut(s) 3647
AhlI ACTAGT 1 cut(s) 3077
AjnI CCWGG 3 cut(s) 1168, 1260, 1720
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
Alw21I GWGCWC 3 cut(s) 941, 2414, 3169
Alw26I GTCTC 5 cut(s) 2157, 2197, 3009, 3586, 3679
AlwI GGATC 6 cut(s) 1964, 2235, 2248, 2549, 2562, 3078
Ama87I CYCGRG 4 cut(s) 1244, 1905, 2529, 3371
Aor51HI AGCGCT 1 cut(s) 1473
AoxI GGCC 8 cut(s) 19, 46, 73, 157, 169, 201, 232, 2894
ApeKI GCWGC 5 cut(s) 514, 656, 1945, 3175, 3559
ApoI RAATTY 6 cut(s) 389, 676, 1057, 2509, 2846, 2878
AseI ATTAAT 1 cut(s) 507
Asp718I GGTACC 1 cut(s) 2525
AspLEI GCGC 6 cut(s) 274, 300, 322, 501, 576, 1474
AspS9I GGNCC 7 cut(s) 53, 170, 307, 338, 2185, 3383, 3508
AsuC2I CCSGG 2 cut(s) 57, 231
AsuHPI GGTGA 9 cut(s) 431, 467, 795, 1250, 1454, 1920, 1952, 2588, 2628
AsuII TTCGAA 1 cut(s) 2513
AvaI CYCGRG 4 cut(s) 1244, 1905, 2529, 3371
AvaII GGWCC 6 cut(s) 53, 307, 338, 2185, 3383, 3508
AxyI CCTNAGG 1 cut(s) 3554
BaeGI GKGCMC 1 cut(s) 2550
BaeI ACNNNNGTAYC 4 cut(s) 2109, 2142, 3246, 3279
BamHI GGATCC 2 cut(s) 2240, 2554
BanI GGYRCC 3 cut(s) 271, 319, 2525
BanII GRGCYC 5 cut(s) 838, 941, 1457, 2414, 2748
BarI GAAGNNNNNNTAC 2 cut(s) 882, 914
BauI CACGAG 1 cut(s) 3024
Bbv12I GWGCWC 3 cut(s) 941, 2414, 3169
BbvCI CCTCAGC 1 cut(s) 3171
BbvI GCAGC 5 cut(s) 526, 668, 1932, 3187, 3571
BccI CCATC 6 cut(s) 1271, 1754, 2216, 2471, 2905, 3028
BceAI ACGGC 2 cut(s) 188, 195
BcgI CGANNNNNNTGC 2 cut(s) 3410, 3444
BciT130I CCWGG 3 cut(s) 1170, 1262, 1722
BclI TGATCA 4 cut(s) 258, 1288, 1801, 2317
BcnI CCSGG 2 cut(s) 57, 231
BcoDI GTCTC 5 cut(s) 2157, 2197, 3009, 3586, 3679
BcuI ACTAGT 1 cut(s) 3077
BfmI CTRYAG 4 cut(s) 651, 1017, 1374, 2865
BfoI RGCGCY 3 cut(s) 275, 323, 1475
BfrI CTTAAG 1 cut(s) 3647
BfuAI ACCTGC 4 cut(s) 780, 1276, 1342, 2872
BglI GCCNNNNNGGC 2 cut(s) 27, 200
BlpI GCTNAGC 1 cut(s) 570
BmcAI AGTACT 2 cut(s) 970, 2399
Bme1390I CCNGG 5 cut(s) 57, 231, 1170, 1262, 1722
Bme18I GGWCC 6 cut(s) 53, 307, 338, 2185, 3383, 3508
BmeT110I CYCGRG 4 cut(s) 1244, 1905, 2529, 3371
BmgT120I GGNCC 7 cut(s) 53, 170, 307, 338, 2185, 3383, 3508
BmrFI CCNGG 5 cut(s) 57, 231, 1170, 1262, 1722
BmrI ACTGGG 2 cut(s) 1506, 2636
BmsI GCATC 8 cut(s) 191, 336, 1612, 1634, 1708, 1834, 2075, 3220
BmuI ACTGGG 2 cut(s) 1506, 2636
BpmI CTGGAG 4 cut(s) 1152, 1854, 2889, 3123
Bpu10I CCTNAGC 4 cut(s) 43, 822, 1932, 3171
Bpu1102I GCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 2513
BpuEI CTTGAG 3 cut(s) 714, 2774, 3145
BpuMI CCSGG 2 cut(s) 57, 231
BsaBI GATNNNNATC 4 cut(s) 1107, 1281, 1848, 3573
BsaHI GRCGYC 2 cut(s) 272, 320
BsaWI WCCGGW 1 cut(s) 1535
BsaXI ACNNNNNCTCC 8 cut(s) 89, 119, 1492, 1522, 1585, 1591, 1615, 1621
Bsc4I CCNNNNNNNGG 9 cut(s) 62, 178, 316, 1746, 1937, 1963, 1979, 2626, 3044
Bse118I RCCGGY 2 cut(s) 62, 199
Bse1I ACTGG 5 cut(s) 763, 862, 1501, 1871, 2631
Bse21I CCTNAGG 1 cut(s) 3554
Bse3DI GCAATG 1 cut(s) 370
Bse8I GATNNNNATC 4 cut(s) 1107, 1281, 1848, 3573
BseBI CCWGG 3 cut(s) 1170, 1262, 1722
BseJI GATNNNNATC 4 cut(s) 1107, 1281, 1848, 3573
BseLI CCNNNNNNNGG 9 cut(s) 62, 178, 316, 1746, 1937, 1963, 1979, 2626, 3044
BseMI GCAATG 1 cut(s) 370
BseNI ACTGG 5 cut(s) 763, 862, 1501, 1871, 2631
BseRI GAGGAG 5 cut(s) 94, 1262, 1334, 1361, 3159
BseSI GKGCMC 1 cut(s) 2550
BseX3I CGGCCG 3 cut(s) 19, 201, 232
BseXI GCAGC 5 cut(s) 526, 668, 1932, 3187, 3571
BsgI GTGCAG 6 cut(s) 533, 906, 957, 1793, 2682, 3020
Bsh1236I CGCG 2 cut(s) 51, 300
Bsh1285I CGRYCG 3 cut(s) 22, 204, 235
BshFI GGCC 8 cut(s) 21, 48, 75, 159, 171, 203, 234, 2896
BshNI GGYRCC 3 cut(s) 271, 319, 2525
BsiEI CGRYCG 3 cut(s) 22, 204, 235
BsiHKAI GWGCWC 3 cut(s) 941, 2414, 3169
BsiHKCI CYCGRG 4 cut(s) 1244, 1905, 2529, 3371
BsiSI CCGG 7 cut(s) 18, 57, 63, 200, 231, 275, 1536
BslFI GGGAC 4 cut(s) 212, 419, 1727, 3396
BslI CCNNNNNNNGG 9 cut(s) 62, 178, 316, 1746, 1937, 1963, 1979, 2626, 3044
BsmAI GTCTC 5 cut(s) 2157, 2197, 3009, 3586, 3679
BsmFI GGGAC 4 cut(s) 212, 419, 1727, 3396
BsmI GAATGC 4 cut(s) 1375, 1423, 2608, 3233
BsnI GGCC 8 cut(s) 21, 48, 75, 159, 171, 203, 234, 2896
BsoBI CYCGRG 4 cut(s) 1244, 1905, 2529, 3371
Bsp119I TTCGAA 1 cut(s) 2513
Bsp1286I GDGCHC 7 cut(s) 838, 941, 1457, 2414, 2550, 2748, 3169
Bsp1720I GCTNAGC 1 cut(s) 570
Bsp19I CCATGG 2 cut(s) 668, 1745
BspANI GGCC 8 cut(s) 21, 48, 75, 159, 171, 203, 234, 2896
BspFNI CGCG 2 cut(s) 51, 300
BspHI TCATGA 2 cut(s) 1804, 3628
BspMAI CTGCAG 1 cut(s) 2869
BspMI ACCTGC 4 cut(s) 780, 1276, 1342, 2872
BspPI GGATC 6 cut(s) 1964, 2235, 2248, 2549, 2562, 3078
BspQI GCTCTTC 2 cut(s) 204, 435
BspT104I TTCGAA 1 cut(s) 2513
BspT107I GGYRCC 3 cut(s) 271, 319, 2525
BspTI CTTAAG 1 cut(s) 3647
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 370
BsrFI RCCGGY 2 cut(s) 62, 199
BsrI ACTGG 5 cut(s) 763, 862, 1501, 1871, 2631
BssAI RCCGGY 2 cut(s) 62, 199
BssNI GRCGYC 2 cut(s) 272, 320
BssSI CACGAG 1 cut(s) 3024
BssT1I CCWWGG 6 cut(s) 310, 668, 1062, 1745, 1998, 2550
Bst2BI CACGAG 1 cut(s) 3024
Bst2UI CCWGG 3 cut(s) 1170, 1262, 1722
Bst6I CTCTTC 4 cut(s) 204, 435, 813, 866
BstACI GRCGYC 2 cut(s) 272, 320
BstAFI CTTAAG 1 cut(s) 3647
BstAPI GCANNNNNTGC 2 cut(s) 779, 1705
BstBI TTCGAA 1 cut(s) 2513
BstC8I GCNNGC 5 cut(s) 64, 68, 201, 930, 2254
BstDSI CCRYGG 5 cut(s) 49, 177, 204, 668, 1745
BstEII GGTNACC 1 cut(s) 419
BstFNI CGCG 2 cut(s) 51, 300
BstH2I RGCGCY 3 cut(s) 275, 323, 1475
BstHHI GCGC 6 cut(s) 274, 300, 322, 501, 576, 1474
BstMAI GTCTC 5 cut(s) 2157, 2197, 3009, 3586, 3679
BstMCI CGRYCG 3 cut(s) 22, 204, 235
BstNI CCWGG 3 cut(s) 1170, 1262, 1722
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 5 cut(s) 55, 229, 1168, 1260, 1720
BstSFI CTRYAG 4 cut(s) 651, 1017, 1374, 2865
BstSLI GKGCMC 1 cut(s) 2550
BstUI CGCG 2 cut(s) 51, 300
BstV1I GCAGC 5 cut(s) 526, 668, 1932, 3187, 3571
BstX2I RGATCY 4 cut(s) 1969, 2240, 2554, 3070
BstYI RGATCY 4 cut(s) 1969, 2240, 2554, 3070
BstZI CGGCCG 3 cut(s) 19, 201, 232
Bsu36I CCTNAGG 1 cut(s) 3554
BsuRI GGCC 8 cut(s) 21, 48, 75, 159, 171, 203, 234, 2896
BtgI CCRYGG 5 cut(s) 49, 177, 204, 668, 1745
BtsIMutI CAGTG 8 cut(s) 560, 993, 1513, 1519, 1552, 2550, 3072, 3304
BveI ACCTGC 4 cut(s) 780, 1276, 1342, 2872
Cac8I GCNNGC 5 cut(s) 64, 68, 201, 930, 2254
CciI TCATGA 2 cut(s) 1804, 3628
CfoI GCGC 6 cut(s) 274, 300, 322, 501, 576, 1474
Cfr10I RCCGGY 2 cut(s) 62, 199
Cfr13I GGNCC 7 cut(s) 53, 170, 307, 338, 2185, 3383, 3508
Cfr42I CCGCGG 1 cut(s) 52
CseI GACGC 2 cut(s) 289, 1823
CspCI CAANNNNNGTGG 2 cut(s) 3105, 3140
DinI GGCGCC 2 cut(s) 273, 321
DraI TTTAAA 2 cut(s) 754, 2293
DrdI GACNNNNNNGTC 1 cut(s) 3671
DseDI GACNNNNNNGTC 1 cut(s) 3671
EaeI YGGCCR 4 cut(s) 19, 73, 201, 232
EagI CGGCCG 3 cut(s) 19, 201, 232
Eam1104I CTCTTC 4 cut(s) 204, 435, 813, 866
EarI CTCTTC 4 cut(s) 204, 435, 813, 866
EciI GGCGGA 2 cut(s) 97, 477
Ecl136II GAGCTC 2 cut(s) 939, 2412
EclXI CGGCCG 3 cut(s) 19, 201, 232
Eco130I CCWWGG 6 cut(s) 310, 668, 1062, 1745, 1998, 2550
Eco24I GRGCYC 5 cut(s) 838, 941, 1457, 2414, 2748
Eco32I GATATC 1 cut(s) 2043
Eco47I GGWCC 6 cut(s) 53, 307, 338, 2185, 3383, 3508
Eco47III AGCGCT 1 cut(s) 1473
Eco52I CGGCCG 3 cut(s) 19, 201, 232
Eco53kI GAGCTC 2 cut(s) 939, 2412
Eco57I CTGAAG 5 cut(s) 1715, 2268, 2811, 3309, 3584
Eco81I CCTNAGG 1 cut(s) 3554
Eco88I CYCGRG 4 cut(s) 1244, 1905, 2529, 3371
Eco91I GGTNACC 1 cut(s) 419
EcoICRI GAGCTC 2 cut(s) 939, 2412
EcoO109I RGGNCCY 3 cut(s) 170, 2185, 3508
EcoO65I GGTNACC 1 cut(s) 419
EcoRI GAATTC 2 cut(s) 1057, 2846
EcoRII CCWGG 3 cut(s) 1168, 1260, 1720
EcoRV GATATC 1 cut(s) 2043
EcoT14I CCWWGG 6 cut(s) 310, 668, 1062, 1745, 1998, 2550
EcoT22I ATGCAT 3 cut(s) 1627, 2424, 3235
EcoT38I GRGCYC 5 cut(s) 838, 941, 1457, 2414, 2748
EgeI GGCGCC 2 cut(s) 273, 321
EheI GGCGCC 2 cut(s) 273, 321
ErhI CCWWGG 6 cut(s) 310, 668, 1062, 1745, 1998, 2550
FalI AAGNNNNNCTT 4 cut(s) 1991, 2023, 2436, 2468
FaqI GGGAC 4 cut(s) 212, 419, 1727, 3396
FauI CCCGC 4 cut(s) 204, 332, 596, 2384
FbaI TGATCA 4 cut(s) 258, 1288, 1801, 2317
FriOI GRGCYC 5 cut(s) 838, 941, 1457, 2414, 2748
GlaI GCGC 6 cut(s) 273, 299, 321, 500, 575, 1473
GsuI CTGGAG 4 cut(s) 1152, 1854, 2889, 3123
HaeII RGCGCY 3 cut(s) 275, 323, 1475
HaeIII GGCC 8 cut(s) 21, 48, 75, 159, 171, 203, 234, 2896
HapII CCGG 7 cut(s) 18, 57, 63, 200, 231, 275, 1536
HgaI GACGC 2 cut(s) 289, 1823
HhaI GCGC 6 cut(s) 274, 300, 322, 501, 576, 1474
Hin1I GRCGYC 2 cut(s) 272, 320
Hin6I GCGC 6 cut(s) 272, 298, 320, 499, 574, 1472
HinP1I GCGC 6 cut(s) 272, 298, 320, 499, 574, 1472
HincII GTYRAC 2 cut(s) 534, 1813
HindII GTYRAC 2 cut(s) 534, 1813
HindIII AAGCTT 2 cut(s) 68, 2912
HpaII CCGG 7 cut(s) 18, 57, 63, 200, 231, 275, 1536
HphI GGTGA 9 cut(s) 431, 467, 795, 1250, 1454, 1920, 1952, 2588, 2628
Hpy166II GTNNAC 6 cut(s) 534, 1357, 1813, 1901, 2636, 2699
Hpy8I GTNNAC 6 cut(s) 534, 1357, 1813, 1901, 2636, 2699
Hpy99I CGWCG 1 cut(s) 305
HpyCH4IV ACGT 2 cut(s) 764, 2124
HpySE526I ACGT 2 cut(s) 764, 2124
Hsp92I GRCGYC 2 cut(s) 272, 320
HspAI GCGC 6 cut(s) 272, 298, 320, 499, 574, 1472
KasI GGCGCC 2 cut(s) 271, 319
KpnI GGTACC 1 cut(s) 2529
KroI GCCGGC 2 cut(s) 62, 199
KroNI GCCGGC 2 cut(s) 64, 201
Ksp22I TGATCA 4 cut(s) 258, 1288, 1801, 2317
KspI CCGCGG 1 cut(s) 52
LguI GCTCTTC 2 cut(s) 204, 435
Lsp1109I GCAGC 5 cut(s) 526, 668, 1932, 3187, 3571
LweI GCATC 8 cut(s) 191, 336, 1612, 1634, 1708, 1834, 2075, 3220
MaeII ACGT 2 cut(s) 764, 2124
MbiI CCGCTC 1 cut(s) 102
MfeI CAATTG 3 cut(s) 1364, 1703, 3117
MflI RGATCY 4 cut(s) 1969, 2240, 2554, 3070
MhlI GDGCHC 7 cut(s) 838, 941, 1457, 2414, 2550, 2748, 3169
Mly113I GGCGCC 2 cut(s) 272, 320
MlyI GAGTC 4 cut(s) 539, 883, 1706, 3176
MmeI TCCRAC 5 cut(s) 647, 836, 1323, 1482, 2285
Mph1103I ATGCAT 3 cut(s) 1627, 2424, 3235
MroNI GCCGGC 2 cut(s) 62, 199
MslI CAYNNNNRTG 5 cut(s) 266, 400, 882, 2085, 3039
MspA1I CMGCKG 2 cut(s) 51, 3562
MspCI CTTAAG 1 cut(s) 3647
MspI CCGG 7 cut(s) 18, 57, 63, 200, 231, 275, 1536
MspR9I CCNGG 5 cut(s) 57, 231, 1170, 1262, 1722
MssI GTTTAAAC 1 cut(s) 2293
MunI CAATTG 3 cut(s) 1364, 1703, 3117
Mva1269I GAATGC 4 cut(s) 1375, 1423, 2608, 3233
MvaI CCWGG 3 cut(s) 1170, 1262, 1722
MvnI CGCG 2 cut(s) 51, 300
NaeI GCCGGC 2 cut(s) 64, 201
NarI GGCGCC 2 cut(s) 272, 320
NciI CCSGG 2 cut(s) 57, 231
NcoI CCATGG 2 cut(s) 668, 1745
NgoMIV GCCGGC 2 cut(s) 62, 199
NmeAIII GCCGAG 2 cut(s) 47, 114
NmuCI GTSAC 9 cut(s) 419, 428, 801, 910, 1502, 1508, 1520, 1541, 1676
NsiI ATGCAT 3 cut(s) 1627, 2424, 3235
NspV TTCGAA 1 cut(s) 2513
PaeR7I CTCGAG 1 cut(s) 1905
PagI TCATGA 2 cut(s) 1804, 3628
PciSI GCTCTTC 2 cut(s) 204, 435
PctI GAATGC 4 cut(s) 1375, 1423, 2608, 3233
PdiI GCCGGC 2 cut(s) 64, 201
PfeI GAWTC 8 cut(s) 482, 1103, 1388, 2234, 2345, 3251, 3317, 3569
PflFI GACNNNGTC 2 cut(s) 281, 2189
PflMI CCANNNNNTGG 1 cut(s) 1963
PleI GAGTC 4 cut(s) 538, 882, 1706, 3176
PluTI GGCGCC 2 cut(s) 275, 323
PmeI GTTTAAAC 1 cut(s) 2293
PpsI GAGTC 4 cut(s) 538, 882, 1706, 3176
PpuMI RGGWCCY 2 cut(s) 2185, 3508
PshBI ATTAAT 1 cut(s) 507
Psp124BI GAGCTC 2 cut(s) 941, 2414
Psp5II RGGWCCY 2 cut(s) 2185, 3508
Psp6I CCWGG 3 cut(s) 1168, 1260, 1720
PspEI GGTNACC 1 cut(s) 419
PspGI CCWGG 3 cut(s) 1168, 1260, 1720
PspPI GGNCC 7 cut(s) 53, 170, 307, 338, 2185, 3383, 3508
PspPPI RGGWCCY 2 cut(s) 2185, 3508
PstI CTGCAG 1 cut(s) 2869
PsuI RGATCY 4 cut(s) 1969, 2240, 2554, 3070
PsyI GACNNNGTC 2 cut(s) 281, 2189
PvuII CAGCTG 1 cut(s) 3562
RseI CAYNNNNRTG 5 cut(s) 266, 400, 882, 2085, 3039
SacI GAGCTC 2 cut(s) 941, 2414
SacII CCGCGG 1 cut(s) 52
SapI GCTCTTC 2 cut(s) 204, 435
Sau96I GGNCC 7 cut(s) 53, 170, 307, 338, 2185, 3383, 3508
SbfI CCTGCAGG 1 cut(s) 2869
ScaI AGTACT 2 cut(s) 970, 2399
SchI GAGTC 4 cut(s) 539, 883, 1706, 3176
ScrFI CCNGG 5 cut(s) 57, 231, 1170, 1262, 1722
SdaI CCTGCAGG 1 cut(s) 2869
SduI GDGCHC 7 cut(s) 838, 941, 1457, 2414, 2550, 2748, 3169
SfaNI GCATC 8 cut(s) 191, 336, 1612, 1634, 1708, 1834, 2075, 3220
SfcI CTRYAG 4 cut(s) 651, 1017, 1374, 2865
SfoI GGCGCC 2 cut(s) 273, 321
Sfr274I CTCGAG 1 cut(s) 1905
Sfr303I CCGCGG 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 2513
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 6 cut(s) 53, 307, 338, 2185, 3383, 3508
SlaI CTCGAG 1 cut(s) 1905
SmiMI CAYNNNNRTG 5 cut(s) 266, 400, 882, 2085, 3039
SmlI CTYRAG 5 cut(s) 729, 1905, 2753, 3160, 3647
SmoI CTYRAG 5 cut(s) 729, 1905, 2753, 3160, 3647
SpeI ACTAGT 1 cut(s) 3077
Sse8387I CCTGCAGG 1 cut(s) 2869
SspDI GGCGCC 2 cut(s) 271, 319
SspI AATATT 2 cut(s) 2230, 3615
SstI GAGCTC 2 cut(s) 941, 2414
StyD4I CCNGG 5 cut(s) 55, 229, 1168, 1260, 1720
StyI CCWWGG 6 cut(s) 310, 668, 1062, 1745, 1998, 2550
TaiI ACGT 2 cut(s) 767, 2127
TaqI TCGA 5 cut(s) 285, 303, 1906, 2513, 3088
TatI WGTACW 5 cut(s) 3, 968, 1991, 2397, 3405
TfiI GAWTC 8 cut(s) 482, 1103, 1388, 2234, 2345, 3251, 3317, 3569
TscAI CASTG 8 cut(s) 560, 993, 1513, 1519, 1552, 2550, 3072, 3304
TseFI GTSAC 9 cut(s) 419, 428, 801, 910, 1502, 1508, 1520, 1541, 1676
TseI GCWGC 5 cut(s) 514, 656, 1945, 3175, 3559
Tsp45I GTSAC 9 cut(s) 419, 428, 801, 910, 1502, 1508, 1520, 1541, 1676
TspGWI ACGGA 3 cut(s) 244, 330, 1067
TspRI CASTG 8 cut(s) 560, 993, 1513, 1519, 1552, 2550, 3072, 3304
Tth111I GACNNNGTC 2 cut(s) 281, 2189
Van91I CCANNNNNTGG 1 cut(s) 1963
Vha464I CTTAAG 1 cut(s) 3647
VpaK11BI GGWCC 6 cut(s) 53, 307, 338, 2185, 3383, 3508
VspI ATTAAT 1 cut(s) 507
XapI RAATTY 6 cut(s) 389, 676, 1057, 2509, 2846, 2878
XcmI CCANNNNNNNNNTGG 2 cut(s) 2267, 2623
XhoI CTCGAG 1 cut(s) 1905
ZrmI AGTACT 2 cut(s) 970, 2399
Zsp2I ATGCAT 3 cut(s) 1627, 2424, 3235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.