MD09G1200300.v1.1

Belongs to the universal ribosomal protein uS9 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013162
Physical Location & Seq
Reverse (-)
18351473 .. 18354001
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1200300.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGCTCTCTCGTCTTGTGCCCAAACCCTCACATTTCTCTCGCTTAACCCTAATCTCCCCAAAGTTCCATCCCTTACCCCAACCCCAAAACCCTCTTTTCACTACTTTCAATCCAAGGTTCTTCTCCAGTGACGATGCTCAGGGTCCCTCCCAATCAGATGTTTGGAGGCTTTCTCAGGAAACCGATGGAAAGTTCGACCCTTTATTCGGCGGGAGCTCCGGGGACATTTCCGGGATTGCAGAAGTTGAGTCCGGCGAGGCCGATTCCGATTGGTTGAAAGGGAGAGCAGGCGAGGAGTGGGGTACATCGCAGGAGTACAAGCTGTGGAGCTTGGGAGAGGAGGAGAAGAGTGCCTTGTTCGATATCGGAGAGGGTGTACAGACTGTTACTGCCGTTGATGGTGAGACAAGGAGAGAGGAGATTGAGAAGGCTGAACAGGCTGAGAGGCTTGAGAAAGAGGAGCGGGAGCTTACTGCTGTTCTCAAAGGTCCGAATCGTGCCTTTGGTGACCTTATTGCTGCATCTGGAATCACAGATGAAATGCTTGACACTTTGATTGCATTGAAGGATTTTCAAGGGATAAGGGGATTGCCCCCTCTATCAGAGATTGAAGACATTCGTTATGAGAAAAATACCAGAAAATCCTCGAGAGCTGAAATAGAGCGCCAGAAACAGGAGGAAGTTGCCAAAGCAAGAGTGAGAATGGTAGACGACAAGGGGAGAGCTTATGGAACAGGAAGAAGGAAATGCAGTGTAGCACGCGTCTGGATTCAACCTGGTAATGGTAAATTCACAATTAATGATAAACAATTTGACGCCTATTTTCCAATGCTTGATCATCGTGCTGCTCTCCTCCGACCTTTCTCTGAGACAAAAACGTTGGGCCTTTGGGACATCGCTTGTACTGTTCAAGGAGGCGGCACCACAGGTCAAGTTGGAGCAATTCAATTAGGAGTGAGCAGGGCCTTGCAAAATTGGGAACCCGACTTGCGTCCTGCACTCAAAGAATCTGGTTTCTTGACAAGGGACTCGCGTGTGGTGGAAAGGAAAAAACCCGGAAAAGCAAAAGCAAGGAAGAGCTTCCAGTGGGTCAAGCGTTGA

Protein Analysis

367

Amino Acids

40.96

Weight (kDa)

6.89

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S9 PF00380 246 - 366 3e-44 Ribosomal protein S9/S16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49080
fragaria_vesca FvH4_6g32580
malus_domestica MD09G1200300.v1.1 MD15G1412900.v1.1
prunus_persica Prupe.3G056900_v2.0.a1
pyrus_communis pycom09g11790 pycom15g36770
rosa_chinensis RchiOBHm_Chr2g0141031
rosa_laevigata RLG00000019909
rosa_multiflora Rmu_sc0004769.1_g000015 Rmu_sc0012897.1_g000007
rosa_roxburghii Rroxscaffold_2G00104270
rosa_rugosa Rorug02G0366200
rosa_samantha Rh2AG417600 Rh2BG427500 Rh2CG403900 Rh2DG437000
rosa_wichuraiana Rw2G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 920
AccBSI CCGCTC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 708
AccII CGCG 2 cut(s) 762, 1033
AciI CCGC 3 cut(s) 210, 463, 918
AclI AACGTT 1 cut(s) 878
AcsI RAATTY 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 816
AfaI GTAC 4 cut(s) 304, 317, 378, 904
AfiI CCNNNNNNNGG 3 cut(s) 206, 673, 782
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 8 cut(s) 109, 277, 565, 575, 611, 773, 911, 947
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 7 cut(s) 216, 322, 330, 469, 653, 725, 1080
AluI AGCT 7 cut(s) 216, 322, 330, 469, 653, 725, 1080
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 2 cut(s) 398, 863
Ama87I CYCGRG 1 cut(s) 646
AoxI GGCC 3 cut(s) 258, 883, 963
ApeKI GCWGC 2 cut(s) 518, 845
ApoI RAATTY 1 cut(s) 788
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AseI ATTAAT 1 cut(s) 798
Asp700I GAANNNNTTC 2 cut(s) 615, 1079
AspLEI GCGC 1 cut(s) 666
AspS9I GGNCC 4 cut(s) 143, 488, 883, 963
AsuC2I CCSGG 3 cut(s) 220, 232, 1056
AsuHPI GGTGA 2 cut(s) 413, 518
AvaI CYCGRG 1 cut(s) 646
AvaII GGWCC 2 cut(s) 143, 488
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 618
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 505, 832
BccI CCATC 3 cut(s) 75, 179, 392
BceAI ACGGC 1 cut(s) 377
BciT130I CCWGG 1 cut(s) 777
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 3 cut(s) 220, 232, 1056
BcoDI GTCTC 2 cut(s) 398, 863
BfoI RGCGCY 1 cut(s) 667
BisI GCNGC 3 cut(s) 519, 846, 919
BlsI GCNGC 3 cut(s) 520, 847, 920
Bme1390I CCNGG 4 cut(s) 220, 232, 777, 1056
Bme18I GGWCC 2 cut(s) 143, 488
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 4 cut(s) 143, 488, 883, 963
BmiI GGNNCC 4 cut(s) 144, 145, 922, 981
BmrFI CCNGG 4 cut(s) 220, 232, 777, 1056
BmsI GCATC 2 cut(s) 124, 530
BoxI GACNNNNGTC 1 cut(s) 990
BpiI GAAGAC 1 cut(s) 618
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 3 cut(s) 220, 232, 1056
BsaHI GRCGYC 1 cut(s) 816
BsaJI CCNNGG 2 cut(s) 113, 219
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 673, 782
Bse1I ACTGG 2 cut(s) 126, 1084
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 113, 219
BseGI GGATG 1 cut(s) 67
BseLI CCNNNNNNNGG 3 cut(s) 206, 673, 782
BseMII CTCAG 4 cut(s) 152, 188, 432, 858
BseNI ACTGG 2 cut(s) 126, 1084
BseRI GAGGAG 6 cut(s) 308, 353, 356, 431, 473, 842
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 2 cut(s) 505, 832
BsgI GTGCAG 1 cut(s) 981
Bsh1236I CGCG 2 cut(s) 762, 1033
BshFI GGCC 3 cut(s) 260, 885, 965
BshNI GGYRCC 1 cut(s) 920
BsiHKAI GWGCWC 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 646
BsiSI CCGG 4 cut(s) 219, 231, 252, 1056
BslFI GGGAC 4 cut(s) 129, 236, 905, 1040
BslI CCNNNNNNNGG 3 cut(s) 206, 673, 782
BsmAI GTCTC 2 cut(s) 398, 863
BsmFI GGGAC 4 cut(s) 129, 236, 905, 1040
BsnI GGCC 3 cut(s) 260, 885, 965
BsoBI CYCGRG 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 21, 218
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 1 cut(s) 835
BspACI CCGC 3 cut(s) 210, 463, 918
BspANI GGCC 3 cut(s) 260, 885, 965
BspCNI CTCAG 4 cut(s) 151, 187, 433, 859
BspFNI CGCG 2 cut(s) 762, 1033
BspLI GGNNCC 4 cut(s) 144, 145, 922, 981
BspQI GCTCTTC 1 cut(s) 1070
BspT107I GGYRCC 1 cut(s) 920
BsrBI CCGCTC 1 cut(s) 463
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 2 cut(s) 126, 1084
BssECI CCNNGG 2 cut(s) 113, 219
BssMI GATC 1 cut(s) 835
BssNI GRCGYC 1 cut(s) 816
BssT1I CCWWGG 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 2 cut(s) 385, 907
Bst6I CTCTTC 2 cut(s) 341, 1070
BstACI GRCGYC 1 cut(s) 816
BstAUI TGTACA 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 289, 760
BstDEI CTNAG 4 cut(s) 138, 174, 441, 867
BstEII GGTNACC 1 cut(s) 506
BstF5I GGATG 1 cut(s) 67
BstFNI CGCG 2 cut(s) 762, 1033
BstH2I RGCGCY 1 cut(s) 667
BstHHI GCGC 1 cut(s) 666
BstKTI GATC 1 cut(s) 838
BstMAI GTCTC 2 cut(s) 398, 863
BstMBI GATC 1 cut(s) 835
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 1 cut(s) 777
BstPAI GACNNNNGTC 1 cut(s) 990
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 4 cut(s) 218, 230, 775, 1054
BstSLI GKGCMC 1 cut(s) 21
BstUI CGCG 2 cut(s) 762, 1033
BstV1I GCAGC 2 cut(s) 505, 832
BstV2I GAAGAC 1 cut(s) 618
BsuRI GGCC 3 cut(s) 260, 885, 965
BtgZI GCGATG 2 cut(s) 291, 880
BtsCI GGATG 1 cut(s) 67
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 3 cut(s) 133, 757, 1091
Cac8I GCNNGC 2 cut(s) 289, 760
CfoI GCGC 1 cut(s) 666
Cfr13I GGNCC 4 cut(s) 143, 488, 883, 963
CseI GACGC 3 cut(s) 751, 824, 980
CsiI ACCWGGT 1 cut(s) 775
Csp6I GTAC 4 cut(s) 303, 316, 377, 903
CviQI GTAC 4 cut(s) 303, 316, 377, 903
DdeI CTNAG 4 cut(s) 138, 174, 441, 867
DpnI GATC 1 cut(s) 837
DpnII GATC 1 cut(s) 835
Eam1104I CTCTTC 2 cut(s) 341, 1070
EarI CTCTTC 2 cut(s) 341, 1070
Ecl136II GAGCTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 218
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 143, 488
Eco53kI GAGCTC 1 cut(s) 216
Eco88I CYCGRG 1 cut(s) 646
Eco91I GGTNACC 1 cut(s) 506
EcoICRI GAGCTC 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 143, 963
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 775
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 113
FaiI YATR 2 cut(s) 624, 729
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 4 cut(s) 129, 236, 905, 1040
FauI CCCGC 2 cut(s) 203, 456
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 3 cut(s) 519, 846, 919
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 3 cut(s) 519, 846, 919
GlaI GCGC 1 cut(s) 665
GluI GCNGC 3 cut(s) 519, 846, 919
GsuI CTGGAG 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 667
HaeIII GGCC 3 cut(s) 260, 885, 965
HapII CCGG 4 cut(s) 219, 231, 252, 1056
HgaI GACGC 3 cut(s) 751, 824, 980
HhaI GCGC 1 cut(s) 666
Hin1I GRCGYC 1 cut(s) 816
Hin6I GCGC 1 cut(s) 664
HinP1I GCGC 1 cut(s) 664
HinfI GANTC 7 cut(s) 248, 263, 493, 528, 769, 1007, 1028
HpaII CCGG 4 cut(s) 219, 231, 252, 1056
HphI GGTGA 2 cut(s) 413, 518
Hpy166II GTNNAC 2 cut(s) 377, 709
Hpy188I TCNGA 7 cut(s) 157, 268, 368, 492, 604, 857, 868
Hpy188III TCNNGA 5 cut(s) 176, 525, 648, 766, 1018
Hpy8I GTNNAC 2 cut(s) 377, 709
HpyAV CCTTC 3 cut(s) 421, 559, 735
HpyCH4III ACNGT 2 cut(s) 385, 907
HpyCH4IV ACGT 1 cut(s) 878
HpyCH4V TGCA 6 cut(s) 239, 521, 560, 750, 970, 998
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 4 cut(s) 138, 174, 441, 867
HpySE526I ACGT 1 cut(s) 878
Hsp92I GRCGYC 1 cut(s) 816
HspAI GCGC 1 cut(s) 664
KflI GGGWCCC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 1 cut(s) 835
LguI GCTCTTC 1 cut(s) 1070
LmnI GCTCC 6 cut(s) 213, 221, 327, 460, 466, 938
Lsp1109I GCAGC 2 cut(s) 505, 832
LweI GCATC 2 cut(s) 124, 530
MabI ACCWGGT 1 cut(s) 775
MaeII ACGT 1 cut(s) 878
MaeIII GTNAC 3 cut(s) 128, 385, 506
MalI GATC 1 cut(s) 837
MbiI CCGCTC 1 cut(s) 463
MboI GATC 1 cut(s) 835
MboII GAAGA 5 cut(s) 112, 358, 623, 750, 1087
MhlI GDGCHC 2 cut(s) 21, 218
MluCI AATT 6 cut(s) 788, 795, 809, 942, 947, 973
MluI ACGCGT 1 cut(s) 760
MlyI GAGTC 2 cut(s) 257, 1022
MmeI TCCRAC 2 cut(s) 880, 916
MroXI GAANNNNTTC 2 cut(s) 615, 1079
MseI TTAA 2 cut(s) 44, 798
MspI CCGG 4 cut(s) 219, 231, 252, 1056
MspR9I CCNGG 4 cut(s) 220, 232, 777, 1056
MvaI CCWGG 1 cut(s) 777
MvnI CGCG 2 cut(s) 762, 1033
MwoI GCNNNNNNNGC 1 cut(s) 437
NciI CCSGG 3 cut(s) 220, 232, 1056
NdeII GATC 1 cut(s) 835
NlaIV GGNNCC 4 cut(s) 144, 145, 922, 981
NmuCI GTSAC 2 cut(s) 128, 506
PaeR7I CTCGAG 1 cut(s) 646
PciSI GCTCTTC 1 cut(s) 1070
PdmI GAANNNNTTC 2 cut(s) 615, 1079
PfeI GAWTC 5 cut(s) 263, 493, 528, 769, 1007
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 3 cut(s) 520, 847, 920
PleI GAGTC 2 cut(s) 256, 1022
PpsI GAGTC 2 cut(s) 256, 1022
PpuMI RGGWCCY 1 cut(s) 143
PshAI GACNNNNGTC 1 cut(s) 990
PshBI ATTAAT 1 cut(s) 798
Psp124BI GAGCTC 1 cut(s) 218
Psp1406I AACGTT 1 cut(s) 878
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 775
PspEI GGTNACC 1 cut(s) 506
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 4 cut(s) 144, 145, 922, 981
PspPI GGNCC 4 cut(s) 143, 488, 883, 963
PspPPI RGGWCCY 1 cut(s) 143
RsaI GTAC 4 cut(s) 304, 317, 378, 904
RsaNI GTAC 4 cut(s) 303, 316, 377, 903
SacI GAGCTC 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 1070
SaqAI TTAA 2 cut(s) 44, 798
SatI GCNGC 3 cut(s) 519, 846, 919
Sau3AI GATC 1 cut(s) 835
Sau96I GGNCC 4 cut(s) 143, 488, 883, 963
SchI GAGTC 2 cut(s) 257, 1022
ScrFI CCNGG 4 cut(s) 220, 232, 777, 1056
SduI GDGCHC 2 cut(s) 21, 218
SexAI ACCWGGT 1 cut(s) 775
SfaNI GCATC 2 cut(s) 124, 530
Sfr274I CTCGAG 1 cut(s) 646
SinI GGWCC 2 cut(s) 143, 488
SlaI CTCGAG 1 cut(s) 646
SmlI CTYRAG 2 cut(s) 449, 646
SmoI CTYRAG 2 cut(s) 449, 646
Sse9I AATT 6 cut(s) 788, 795, 809, 942, 947, 973
SsiI CCGC 3 cut(s) 210, 463, 918
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 4 cut(s) 218, 230, 775, 1054
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 2 cut(s) 385, 907
TaiI ACGT 1 cut(s) 881
TaqI TCGA 3 cut(s) 195, 360, 647
TasI AATT 6 cut(s) 788, 795, 809, 942, 947, 973
TatI WGTACW 3 cut(s) 315, 376, 902
TauI GCSGC 1 cut(s) 921
TfiI GAWTC 5 cut(s) 263, 493, 528, 769, 1007
Tru1I TTAA 2 cut(s) 44, 798
Tru9I TTAA 2 cut(s) 44, 798
TscAI CASTG 3 cut(s) 133, 757, 1091
TseFI GTSAC 2 cut(s) 128, 506
TseI GCWGC 2 cut(s) 518, 845
Tsp45I GTSAC 2 cut(s) 128, 506
TspDTI ATGAA 1 cut(s) 552
TspRI CASTG 3 cut(s) 133, 757, 1091
VpaK11BI GGWCC 2 cut(s) 143, 488
VspI ATTAAT 1 cut(s) 798
XapI RAATTY 1 cut(s) 788
XhoI CTCGAG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 2 cut(s) 615, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.