MD15G1412900.v1.1

Belongs to the universal ribosomal protein uS9 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013162
Physical Location & Seq
Reverse (-)
51237673 .. 51240423
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1412900.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGCTCTCTCGTCTTCTACCCAAACCCTCACATTTCTCTCGCCTAACCCTAATCTCCCCAAATTTCCATCCCTTCCCCCAACCCCAAAACCCTCTTTTCGCTACTTTCACTCCAAGGTTCTTCTCCAGTGACGATGGTAAGGGTCCCTCCCAATCAGATGTTTGGAGGCTTTCTCAGGAAACCGATGGAAAGTTTGACCTTTTATTCGGCCAGAGCTCCGGGGACATTTCCGGGATTGCAGAAGTTGAATCCAGCGAGGCCGATTCCGACTGGTTGAAGGGGAAAGGAGGCGAGGAGTGGGATACATCGCAGGAGTACAAGATGTGGAGCTTGGGAGAGGAGGCGAAGAGTGACTTGTTCGATATCGGAGAGGGTGTACAGACTGTTACTGTCATTGACAGTGAGACAAGTAGAGAGGAGATTGAGAAGGCTGAACAGGCTAAGAGGCTTGAGAAAGAGGAGCAGGAGCTTACTGCTGTTCTCAAAGGTCCGAATCGTGCCTTTGGTGACCTTATTGCTGCATCTGGAATCACAGACGAAATGCTTGACACTTTGATTGCATTGAAGGATTTTCAAGGGATAAAGGGATTGCCCCCTCTTTCAGAGATTGAAGACATTCGTTATGAGAAAAATACCAGAAAATCCTCGAGAGCTGAAATAGAGCGCCAGAAACAGGAGGAAGTTGCCAAAGCAAGAGTGAGAACAGTAGACGACAAGGGGAGAGCTTATGGAACAGGAAGAAGGAAATGTAGTGTAGCACGTGTCTGGATTCAACCTGGTAATGGTAAATTCACAATTAATGATAAACAATTTGACACCTATTTTCCAATGCTTGATCATCGTGCTGCTCTCCTCCGACCTTTCTCTGAGACAAAAACGTTGGGCCTTTGGGACATCGCTTGTAGTGTCCAAGGAGGTGGCACCACAGGTCAAGTTGGAGCAATTCAATTAGGAGTGAGCAGGGCCTTGCAAAATTGGGAACCTGATTTGCGTCCTGCTCTAAAAGAATCTGGTTTCTTGACAAGGGACTCGCGTGTGGTGGAAAGGAAAAAACCCGGAAAAGCAAAAGCAAGGAAGAGCTTCCAGTGGGTCAAGCGTTGA

Protein Analysis

367

Amino Acids

41.0

Weight (kDa)

6.42

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S9 PF00380 246 - 366 4.8e-44 Ribosomal protein S9/S16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49080
fragaria_vesca FvH4_6g32580
malus_domestica MD09G1200300.v1.1 MD15G1412900.v1.1
prunus_persica Prupe.3G056900_v2.0.a1
pyrus_communis pycom09g11790 pycom15g36770
rosa_chinensis RchiOBHm_Chr2g0141031
rosa_laevigata RLG00000019909
rosa_multiflora Rmu_sc0004769.1_g000015 Rmu_sc0012897.1_g000007
rosa_roxburghii Rroxscaffold_2G00104270
rosa_rugosa Rorug02G0366200
rosa_samantha Rh2AG417600 Rh2BG427500 Rh2CG403900 Rh2DG437000
rosa_wichuraiana Rw2G034180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 920
AccI GTMKAC 1 cut(s) 708
AccII CGCG 1 cut(s) 1033
AclI AACGTT 1 cut(s) 878
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 61, 788
AcvI CACGTG 1 cut(s) 761
AfaI GTAC 2 cut(s) 317, 378
AfiI CCNNNNNNNGG 2 cut(s) 673, 782
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 7 cut(s) 248, 277, 565, 575, 611, 773, 947
AjnI CCWGG 1 cut(s) 775
AluBI AGCT 6 cut(s) 216, 330, 469, 653, 725, 1080
AluI AGCT 6 cut(s) 216, 330, 469, 653, 725, 1080
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 2 cut(s) 398, 863
Ama87I CYCGRG 1 cut(s) 646
AoxI GGCC 4 cut(s) 208, 258, 883, 963
ApeKI GCWGC 2 cut(s) 518, 845
ApoI RAATTY 2 cut(s) 61, 788
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AseI ATTAAT 1 cut(s) 798
Asp700I GAANNNNTTC 2 cut(s) 615, 1079
AspLEI GCGC 1 cut(s) 666
AspS9I GGNCC 4 cut(s) 143, 488, 883, 963
AsuC2I CCSGG 3 cut(s) 220, 232, 1056
AsuHPI GGTGA 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 646
AvaII GGWCC 2 cut(s) 143, 488
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 218
BbrPI CACGTG 1 cut(s) 761
BbsI GAAGAC 2 cut(s) 5, 618
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 505, 832
BccI CCATC 3 cut(s) 75, 128, 179
BciT130I CCWGG 1 cut(s) 777
BciVI GTATCC 1 cut(s) 295
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 3 cut(s) 220, 232, 1056
BcoDI GTCTC 2 cut(s) 398, 863
BfoI RGCGCY 1 cut(s) 667
BfuI GTATCC 1 cut(s) 295
BisI GCNGC 2 cut(s) 519, 846
BlsI GCNGC 2 cut(s) 520, 847
Bme1390I CCNGG 4 cut(s) 220, 232, 777, 1056
Bme18I GGWCC 2 cut(s) 143, 488
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 4 cut(s) 143, 488, 883, 963
BmiI GGNNCC 4 cut(s) 144, 145, 922, 981
BmrFI CCNGG 4 cut(s) 220, 232, 777, 1056
BmsI GCATC 1 cut(s) 530
BpiI GAAGAC 2 cut(s) 5, 618
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 3 cut(s) 220, 232, 1056
BsaAI YACGTR 1 cut(s) 761
BsaJI CCNNGG 3 cut(s) 113, 219, 910
BsaXI ACNNNNNCTCC 4 cut(s) 94, 124, 360, 390
Bsc4I CCNNNNNNNGG 2 cut(s) 673, 782
Bse1I ACTGG 3 cut(s) 126, 275, 1084
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 3 cut(s) 113, 219, 910
BseGI GGATG 1 cut(s) 67
BseLI CCNNNNNNNGG 2 cut(s) 673, 782
BseMII CTCAG 2 cut(s) 188, 858
BseNI ACTGG 3 cut(s) 126, 275, 1084
BseRI GAGGAG 5 cut(s) 308, 353, 431, 473, 842
BseXI GCAGC 2 cut(s) 505, 832
Bsh1236I CGCG 1 cut(s) 1033
BshFI GGCC 4 cut(s) 210, 260, 885, 965
BshNI GGYRCC 1 cut(s) 920
BsiHKAI GWGCWC 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 646
BsiSI CCGG 3 cut(s) 219, 231, 1056
BslFI GGGAC 4 cut(s) 129, 236, 905, 1040
BslI CCNNNNNNNGG 2 cut(s) 673, 782
BsmAI GTCTC 2 cut(s) 398, 863
BsmFI GGGAC 4 cut(s) 129, 236, 905, 1040
BsnI GGCC 4 cut(s) 210, 260, 885, 965
BsoBI CYCGRG 1 cut(s) 646
Bsp1286I GDGCHC 1 cut(s) 218
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 1 cut(s) 835
BspANI GGCC 4 cut(s) 210, 260, 885, 965
BspCNI CTCAG 2 cut(s) 187, 859
BspFNI CGCG 1 cut(s) 1033
BspLI GGNNCC 4 cut(s) 144, 145, 922, 981
BspQI GCTCTTC 1 cut(s) 1070
BspT107I GGYRCC 1 cut(s) 920
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 3 cut(s) 126, 275, 1084
BssECI CCNNGG 3 cut(s) 113, 219, 910
BssMI GATC 1 cut(s) 835
BssT1I CCWWGG 2 cut(s) 113, 910
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 4 cut(s) 385, 391, 401, 706
Bst6I CTCTTC 2 cut(s) 341, 1070
BstAUI TGTACA 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 761
BstDEI CTNAG 3 cut(s) 174, 441, 867
BstEII GGTNACC 1 cut(s) 506
BstF5I GGATG 1 cut(s) 67
BstFNI CGCG 1 cut(s) 1033
BstH2I RGCGCY 1 cut(s) 667
BstHHI GCGC 1 cut(s) 666
BstKTI GATC 1 cut(s) 838
BstMAI GTCTC 2 cut(s) 398, 863
BstMBI GATC 1 cut(s) 835
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 1 cut(s) 777
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 4 cut(s) 218, 230, 775, 1054
BstUI CGCG 1 cut(s) 1033
BstV1I GCAGC 2 cut(s) 505, 832
BstV2I GAAGAC 2 cut(s) 5, 618
BstXI CCANNNNNNTGG 1 cut(s) 917
BsuI GTATCC 1 cut(s) 295
BsuRI GGCC 4 cut(s) 210, 260, 885, 965
BtgZI GCGATG 2 cut(s) 291, 880
BtsCI GGATG 1 cut(s) 67
BtsIMutI CAGTG 3 cut(s) 133, 406, 1091
CfoI GCGC 1 cut(s) 666
Cfr13I GGNCC 4 cut(s) 143, 488, 883, 963
CseI GACGC 1 cut(s) 980
CsiI ACCWGGT 1 cut(s) 775
Csp6I GTAC 2 cut(s) 316, 377
CviQI GTAC 2 cut(s) 316, 377
DdeI CTNAG 3 cut(s) 174, 441, 867
DpnI GATC 1 cut(s) 837
DpnII GATC 1 cut(s) 835
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 2 cut(s) 341, 1070
EarI CTCTTC 2 cut(s) 341, 1070
Ecl136II GAGCTC 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 113, 910
Eco24I GRGCYC 1 cut(s) 218
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 143, 488
Eco53kI GAGCTC 1 cut(s) 216
Eco72I CACGTG 1 cut(s) 761
Eco88I CYCGRG 1 cut(s) 646
Eco91I GGTNACC 1 cut(s) 506
EcoICRI GAGCTC 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 143, 963
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 775
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 2 cut(s) 113, 910
EcoT38I GRGCYC 1 cut(s) 218
ErhI CCWWGG 2 cut(s) 113, 910
FaiI YATR 2 cut(s) 624, 729
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 4 cut(s) 129, 236, 905, 1040
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 2 cut(s) 519, 846
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 2 cut(s) 519, 846
GlaI GCGC 1 cut(s) 665
GluI GCNGC 2 cut(s) 519, 846
GsuI CTGGAG 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 667
HaeIII GGCC 4 cut(s) 210, 260, 885, 965
HapII CCGG 3 cut(s) 219, 231, 1056
HgaI GACGC 1 cut(s) 980
HhaI GCGC 1 cut(s) 666
Hin6I GCGC 1 cut(s) 664
HinP1I GCGC 1 cut(s) 664
HinfI GANTC 7 cut(s) 248, 263, 493, 528, 769, 1007, 1028
HpaII CCGG 3 cut(s) 219, 231, 1056
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 2 cut(s) 377, 709
Hpy188I TCNGA 7 cut(s) 157, 268, 368, 492, 604, 857, 868
Hpy188III TCNNGA 5 cut(s) 176, 525, 648, 766, 1018
Hpy8I GTNNAC 2 cut(s) 377, 709
HpyAV CCTTC 5 cut(s) 82, 271, 421, 559, 735
HpyCH4III ACNGT 4 cut(s) 385, 391, 401, 706
HpyCH4IV ACGT 2 cut(s) 760, 878
HpyCH4V TGCA 4 cut(s) 239, 521, 560, 970
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 3 cut(s) 174, 441, 867
HpySE526I ACGT 2 cut(s) 760, 878
HspAI GCGC 1 cut(s) 664
KflI GGGWCCC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 1 cut(s) 835
LguI GCTCTTC 1 cut(s) 1070
LmnI GCTCC 5 cut(s) 221, 327, 460, 466, 938
Lsp1109I GCAGC 2 cut(s) 505, 832
LweI GCATC 1 cut(s) 530
MabI ACCWGGT 1 cut(s) 775
MaeII ACGT 2 cut(s) 760, 878
MaeIII GTNAC 4 cut(s) 128, 350, 385, 506
MalI GATC 1 cut(s) 837
MboI GATC 1 cut(s) 835
MboII GAAGA 6 cut(s) 5, 112, 358, 623, 750, 1087
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 7 cut(s) 61, 788, 795, 809, 942, 947, 973
MlyI GAGTC 1 cut(s) 1022
MmeI TCCRAC 3 cut(s) 291, 880, 916
MroXI GAANNNNTTC 2 cut(s) 615, 1079
MseI TTAA 1 cut(s) 798
MspI CCGG 3 cut(s) 219, 231, 1056
MspR9I CCNGG 4 cut(s) 220, 232, 777, 1056
MvaI CCWGG 1 cut(s) 777
MvnI CGCG 1 cut(s) 1033
MwoI GCNNNNNNNGC 1 cut(s) 437
NciI CCSGG 3 cut(s) 220, 232, 1056
NdeII GATC 1 cut(s) 835
NlaIV GGNNCC 4 cut(s) 144, 145, 922, 981
NmuCI GTSAC 3 cut(s) 128, 350, 506
PaeR7I CTCGAG 1 cut(s) 646
PciSI GCTCTTC 1 cut(s) 1070
PdmI GAANNNNTTC 2 cut(s) 615, 1079
PfeI GAWTC 6 cut(s) 248, 263, 493, 528, 769, 1007
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 2 cut(s) 520, 847
PleI GAGTC 1 cut(s) 1022
PmaCI CACGTG 1 cut(s) 761
PmlI CACGTG 1 cut(s) 761
PpsI GAGTC 1 cut(s) 1022
Ppu21I YACGTR 1 cut(s) 761
PpuMI RGGWCCY 1 cut(s) 143
PshBI ATTAAT 1 cut(s) 798
Psp124BI GAGCTC 1 cut(s) 218
Psp1406I AACGTT 1 cut(s) 878
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 775
PspCI CACGTG 1 cut(s) 761
PspEI GGTNACC 1 cut(s) 506
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 4 cut(s) 144, 145, 922, 981
PspPI GGNCC 4 cut(s) 143, 488, 883, 963
PspPPI RGGWCCY 1 cut(s) 143
RsaI GTAC 2 cut(s) 317, 378
RsaNI GTAC 2 cut(s) 316, 377
SacI GAGCTC 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 1070
SaqAI TTAA 1 cut(s) 798
SatI GCNGC 2 cut(s) 519, 846
Sau3AI GATC 1 cut(s) 835
Sau96I GGNCC 4 cut(s) 143, 488, 883, 963
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 4 cut(s) 220, 232, 777, 1056
SduI GDGCHC 1 cut(s) 218
SexAI ACCWGGT 1 cut(s) 775
SfaNI GCATC 1 cut(s) 530
Sfr274I CTCGAG 1 cut(s) 646
SinI GGWCC 2 cut(s) 143, 488
SlaI CTCGAG 1 cut(s) 646
SmlI CTYRAG 2 cut(s) 449, 646
SmoI CTYRAG 2 cut(s) 449, 646
Sse9I AATT 7 cut(s) 61, 788, 795, 809, 942, 947, 973
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 4 cut(s) 218, 230, 775, 1054
StyI CCWWGG 2 cut(s) 113, 910
TaaI ACNGT 4 cut(s) 385, 391, 401, 706
TaiI ACGT 2 cut(s) 763, 881
TaqI TCGA 2 cut(s) 360, 647
TasI AATT 7 cut(s) 61, 788, 795, 809, 942, 947, 973
TatI WGTACW 2 cut(s) 315, 376
TfiI GAWTC 6 cut(s) 248, 263, 493, 528, 769, 1007
Tru1I TTAA 1 cut(s) 798
Tru9I TTAA 1 cut(s) 798
TscAI CASTG 3 cut(s) 133, 406, 1091
TseFI GTSAC 3 cut(s) 128, 350, 506
TseI GCWGC 2 cut(s) 518, 845
Tsp45I GTSAC 3 cut(s) 128, 350, 506
TspRI CASTG 3 cut(s) 133, 406, 1091
VpaK11BI GGWCC 2 cut(s) 143, 488
VspI ATTAAT 1 cut(s) 798
XapI RAATTY 2 cut(s) 61, 788
XhoI CTCGAG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 2 cut(s) 615, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.