Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004830

Physical Location & Seq

Genomic Coordinates Forward (+)

20095706 .. 20106627

Gene Structure 10922 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1208900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2946 bp

ATGGCGTGGTTCAGTGGGAAAGTCTCTTTGGGGAACTTTCCCGATCTCGCCGGGGCCGTGAATAAGCTCCAGGAGAGCGTGAAGAACATCGAGAAGAATTTCGATAGTGCCCTCGGATTCGAAGAAAGAGAGAAAGCCGAATCCGGCCATGAAGCTTCAGGATTATGGCCTTCATCTAGTGAAAGGATTATGTCCTTTATGGGGCAACAAAATGAGGAAAGTAATGTTGAATCATCAGAGAAAGCCGAGTCTTCTGATTCTCCACCTAAGGTTGACAAATCACCTGGGGAAACTGAATCTCTACAGCTAACGTCTACTGTTGAGGATAAAGAAGGGGTCAAGGCTGAAACTTTGCAACAATCTACAACTGAACAGATGGCTGCTAAGGAGGAAAATGAAGTTGTAAAGGAAGAAAAAGATGATAACCCTGCTGCGGTTGTGGGGGAAACAAAGACTGTGATAGCAGAGTCTGAAAAATCTGAATCCGAGTCACCTTCAGTGCTGGTTGAACAACCTGAATCCACTGTTAAGAATGCTGGCCCATCCGATTCTGTTTATTCTCAAGAAGACAACAAGATTTCTGTAGCGGGACCCCCAGAAAATTCAGAATCATTGCAAGAAAAGTCAGGATTTCTTGCAGTAGATCAAGTTGAGGAAGGTAGTACTGCTCTGCTGGGTGAGGAACATTATATAGTCGATATGCATGAGAATTTGGATGAACATAAACCACAAGTAGAGAAGGATGGACATATGACACAGGTTGAAGAGAATGTTGATATGATTTCTCCAGTCAAAGCTGAGCCATCGACTGATAGTCAGCCTGGAGGTTTGGATGAGCCCTCCGTTGAGATTCATACCGTTGGTAGATTATCTACTAATCAACTACCCAGTGTGCATCATTCTGATGATGCTTCGCATACCGTTTCTGAATTGGCTTTGAAAGAGCATAATGCTGTTGTTGAGGAACCTGAAGTTGATCAACGAGCTGATGAGAATGAAGCTGACGTTAAAGAGCAGCATTTGAGCTCAGGAGAAAATGCATCTGACTCTTCAGAGGCTTTGATTGAATTGGAGAAGCTAAAGGTGGAGATGAAAATGATGGAAGCTGCATTGCAAGGTGCTGCAAGACAAGCTCAGGCAAAAGCTGATGAGATTGCAAAGTTTATGAACGAAAATGAGCAATTAAAAGCTGCAATTGAGGATTTGAAGAGAAAATCCAGTGATGCAGAAGTTGAGTCTCTGCGAGAGGAATATCATCAAAGGGTTGCAACACTTGAAAGGAAGGTTTATGCTCTTACTAAGGAAAGGGACACGCTTCGGCGGGAACAGAATAAGAAAAGTGACGCTGCTGCTCTTCTGAAGGAAAAGGATGAAATAATTAATCAAGTTATGGCTGAAGGCGAAGAGCTATCAAAAAAGCAGGCTGCTCAAGAAGCTCAAATTAGGAAATTAAGGGCTCAGATCAGAGAGTTTGAAGAAGAGAAGAAAGGGCTGAGTACTAAACTTCAGGTTGAAGAGAATAAAGTAGAGAGCATTAAGAGGGACAAGATGGCTACAGAAAAGTTGCTGCAGGAGACCATTGAAAAGCACCAAATGGAACTTGCATCACAGAAAGAGTATTATACTAATGCTTTGGCTGCTGCCAAGGAGGCAGAAGCTTTGGCCGAGGCACGTGCCAACGATGAAGCCAGATCGGAACTAGAGAGGCGTCTAAAAGAGGCTGAGGAACGTGAGGCTTTGCTAGTTCAGGCACTTGAAGAATTGCGGCAGACTCTAACTAGAAAGGAGCAGCAGGCAGTATTTAGAGAAGACATGCTTCTCCGTGACATTGAGGATCTTCAGAAACATTATCAAGCAAGTGAGCGCCGATGTGAGGAGTTGATTACCCAAGTACCGGAATCTACCAGGCCTCTTCTTAGGCAGATTGAAGCTATGCAGGAAACAAATTCAAGAAGGGCAGAAGCTTGGGCAGCTGTTGAGAGGTCTCTTAACTCTCGACTCCAGGAAGCAGAGGCCAAAGCTGCAGCTGCTGAGGAAAGAGAACGATCTGTTAATGAGCGCTTGTCTCAAACGTTATCTAGAATTAACGTCCTTGAGGCTCAGATTTCCTGTCTTAGAGCTGAGCAATCCCAGTTAAGTAAGTCTGTTGAAAAGGAGAGACAAAGAGCAGCTGAAAATAGGCAGGAGTACCTTGCAGCAAAGGAGGAAGCTGACACTCAAGAAGGCCGTGCAAACCAGCTTGAAGAAGAAATTAGGGAACTCAGGCGAAAACATAAGCAAGATTTACAAGATGCATTGATGCATAGGGAACTGCTACAGCAGGAGGTAGAAAGGGAGAAAGCTGCTAAGTTAGAGTTGGAAAAGACAGCTCGTGTCCGCTCTGCTACTGTATCTGAACAGACTACTATAACAAGGCACAATTCGGCTTTAGAGAATGGTGTGATTTGCTTGAGCCGAAAACTCTCAAGTGCTAGCAGCCTGGGGAGTATGGAGGAAAGCTATTTTCTGCAAGCATCTTTGGACTCATCCGATGGTTTCTCTGAGAGGAGAAATGCTGGAGAGGCAACTATGAGTCCGTACTATATGAAGAGTATGACACCTAGTGCTTTTGAAGCTTCTCTTCGTCAGAAGGAGGGTGAACTTGCATCTTACATGTCTCGTTTGGCATCCATGGAATCTATTCGTGATTCTCTTGCTGAGGAGTTAGTCAAAATGACAGAACAGTGTGAAAAGTTACGGGCAGAGGCTGGCATGTTGCCTGGTATGCGAGCAGAGCTAGAGGCACTAAGGAGACGGCACTCTGCTGCACTGGAGCTAATGGGCGAACGTGATGAGGAGCTGGAGGAGCTTCGTGCGGATATTGTGGACTTGAAGGAGATGTATAGAGAGCAAGTGAACTTGCTTGTCAACAAGATCCAGATAATGAGTTCATCGGTTGGAGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TMF_DNA_bd TMF_TATA_bd

Protein Analysis

982

Amino Acids

110.26

Weight (kDa)

4.82

Isoelectric Point (pI)

65.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TMF_DNA_bd	PF12329	448 - 515	1.1e-14	TATA element modulatory factor 1 DNA binding
TMF_TATA_bd	PF12325	864 - 967	4.6e-28	TATA element modulatory factor 1 TATA binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004830)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79830 AT1G79830 AT1G79830 AT1G79830 AT1G79830
fragaria_vesca	FvH4_6g31822 FvH4_6g31822 FvH4_6g31822 FvH4_6g31822 FvH4_6g31822 FvH4_6g31822
malus_domestica	MD09G1208900.v1.1 MD17G1190500.v1.1
prunus_persica	Prupe.2G059800_v2.0.a1 Prupe.3G066400_v2.0.a1 Prupe.3G066400_v2.0.a1 Prupe.4G118900_v2.0.a1 Prupe.4G118900_v2.0.a1 Prupe.5G245900_v2.0.a1 Prupe.5G245900_v2.0.a1
pyrus_communis	pycom09g12520
rosa_chinensis	RchiOBHm_Chr2g0139071
rosa_laevigata	RLG00000019788
rosa_multiflora	Rmu_sc0006014.1_g000014
rosa_roxburghii	Rroxscaffold_2G00105700
rosa_rugosa	Rorug02G0354600
rosa_samantha	Rh2AG404600 Rh2BG414600 Rh2CG390900 Rh2DG424300
rosa_wichuraiana	Rw2G033050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	2379
AccI	GTMKAC	1 cut(s)	314
AciI	CCGC	6 cut(s)	434, 587, 1321, 1765, 2377, 2855
AclI	AACGTT	1 cut(s)	2072
AclWI	GGATC	2 cut(s)	1842, 2908
AcoI	YGGCCR	2 cut(s)	145, 1662
AcsI	RAATTY	4 cut(s)	97, 601, 709, 1945
AcuI	CTGAAG	8 cut(s)	141, 480, 990, 1035, 1379, 1416, 1490, 1823
AcvI	CACGTG	1 cut(s)	1673
AcyI	GRCGYC	1 cut(s)	1708
AdeI	CACNNNGTG	1 cut(s)	2603
AfaI	GTAC	5 cut(s)	664, 1498, 1893, 2189, 2579
AfeI	AGCGCT	1 cut(s)	2060
AfiI	CCNNNNNNNGG	4 cut(s)	201, 433, 1873, 1894
AflIII	ACRYGT	1 cut(s)	2652
AhdI	GACNNNNNGTC	1 cut(s)	813
AjnI	CCWGG	7 cut(s)	69, 283, 820, 1904, 2001, 2478, 2758
AjuI	GAANNNNNNNTTGG	2 cut(s)	11, 43
Alw21I	GWGCWC	1 cut(s)	1028
Alw26I	GTCTC	8 cut(s)	28, 1242, 1568, 1989, 2070, 2152, 2661, 2785
AlwI	GGATC	2 cut(s)	1842, 2908
AlwNI	CAGNNNCTG	3 cut(s)	470, 2030, 2747
Aor51HI	AGCGCT	1 cut(s)	2060
AoxI	GGCC	8 cut(s)	54, 145, 167, 538, 1662, 1907, 2013, 2224
ApoI	RAATTY	4 cut(s)	97, 601, 709, 1945
AseI	ATTAAT	1 cut(s)	1380
Asp700I	GAANNNNTTC	2 cut(s)	98, 2679
AspLEI	GCGC	2 cut(s)	1866, 2061
AspS9I	GGNCC	3 cut(s)	54, 539, 590
AsuC2I	CCSGG	1 cut(s)	52
AsuHPI	GGTGA	4 cut(s)	273, 483, 689, 2648
AsuII	TTCGAA	1 cut(s)	120
AsuNHI	GCTAGC	1 cut(s)	2471
AvaII	GGWCC	1 cut(s)	590
AxyI	CCTNAGG	1 cut(s)	267
BaeGI	GKGCMC	1 cut(s)	112
BaeI	ACNNNNGTAYC	2 cut(s)	1875, 1908
BanII	GRGCYC	3 cut(s)	840, 1028, 1459
BauI	CACGAG	1 cut(s)	2370
BbrPI	CACGTG	1 cut(s)	1673
BbsI	GAAGAC	3 cut(s)	243, 573, 1815
Bbv12I	GWGCWC	1 cut(s)	1028
BbvCI	CCTCAGC	3 cut(s)	1722, 2031, 2697
BccI	CCATC	7 cut(s)	370, 550, 737, 811, 1093, 1543, 2525
BceAI	ACGGC	3 cut(s)	41, 2211, 2810
BcgI	CGANNNNNNTGC	2 cut(s)	1655, 1689
BciT130I	CCWGG	7 cut(s)	71, 285, 822, 1906, 2003, 2480, 2760
BclI	TGATCA	1 cut(s)	976
BcnI	CCSGG	1 cut(s)	52
BcoDI	GTCTC	8 cut(s)	28, 1242, 1568, 1989, 2070, 2152, 2661, 2785
BfaI	CTAG	9 cut(s)	177, 1700, 1742, 1779, 2079, 2472, 2601, 2777, 2944
BfmI	CTRYAG	6 cut(s)	302, 582, 1554, 1568, 2022, 2315
BfoI	RGCGCY	2 cut(s)	1867, 2062
BglI	GCCNNNNNGGC	1 cut(s)	1649
BlpI	GCTNAGC	2 cut(s)	798, 2121
BmcAI	AGTACT	2 cut(s)	664, 1498
Bme1390I	CCNGG	8 cut(s)	52, 71, 285, 822, 1906, 2003, 2480, 2760
Bme18I	GGWCC	1 cut(s)	590
BmeRI	GACNNNNNGTC	1 cut(s)	813
BmgT120I	GGNCC	3 cut(s)	54, 539, 590
BmiI	GGNNCC	5 cut(s)	55, 591, 592, 966, 2941
BmrFI	CCNGG	8 cut(s)	52, 71, 285, 822, 1906, 2003, 2480, 2760
BmrI	ACTGGG	2 cut(s)	882, 2125
BmtI	GCTAGC	1 cut(s)	2475
BmuI	ACTGGG	2 cut(s)	882, 2125
BpiI	GAAGAC	3 cut(s)	243, 573, 1815
BpmI	CTGGAG	7 cut(s)	53, 771, 843, 1985, 2577, 2831, 2861
Bpu10I	CCTNAGC	6 cut(s)	384, 1027, 1134, 1722, 2031, 2697
Bpu1102I	GCTNAGC	2 cut(s)	798, 2121
Bpu14I	TTCGAA	1 cut(s)	120
BpuEI	CTTGAG	6 cut(s)	546, 1413, 2114, 2202, 2449, 2470
BpuMI	CCSGG	1 cut(s)	52
BsaAI	YACGTR	1 cut(s)	1673
BsaHI	GRCGYC	1 cut(s)	1708
BsaI	GGTCTC	2 cut(s)	1568, 1989
BsaJI	CCNNGG	7 cut(s)	51, 112, 284, 1644, 1665, 2479, 2670
BsaWI	WCCGGW	1 cut(s)	1894
Bsc4I	CCNNNNNNNGG	4 cut(s)	201, 433, 1873, 1894
Bse1I	ACTGG	5 cut(s)	788, 888, 1218, 2131, 2814
Bse21I	CCTNAGG	1 cut(s)	267
Bse3DI	GCAATG	2 cut(s)	611, 1109
BseBI	CCWGG	7 cut(s)	71, 285, 822, 1906, 2003, 2480, 2760
BseDI	CCNNGG	7 cut(s)	51, 112, 284, 1644, 1665, 2479, 2670
BseGI	GGATG	7 cut(s)	542, 721, 748, 838, 1375, 2525, 2666
BseLI	CCNNNNNNNGG	4 cut(s)	201, 433, 1873, 1894
BseMI	GCAATG	2 cut(s)	611, 1109
BseNI	ACTGG	5 cut(s)	788, 888, 1218, 2131, 2814
BseRI	GAGGAG	5 cut(s)	1889, 2560, 2714, 2849, 2858
BseSI	GKGCMC	1 cut(s)	112
BseYI	CCCAGC	1 cut(s)	673
BsgI	GTGCAG	1 cut(s)	2790
BshFI	GGCC	8 cut(s)	56, 147, 169, 540, 1664, 1909, 2015, 2226
BsiHKAI	GWGCWC	1 cut(s)	1028
BsiSI	CCGG	3 cut(s)	51, 144, 1895
BslFI	GGGAC	3 cut(s)	603, 1322, 1556
BslI	CCNNNNNNNGG	4 cut(s)	201, 433, 1873, 1894
BsmAI	GTCTC	8 cut(s)	28, 1242, 1568, 1989, 2070, 2152, 2661, 2785
BsmBI	CGTCTC	1 cut(s)	2785
BsmFI	GGGAC	3 cut(s)	603, 1322, 1556
BsmI	GAATGC	1 cut(s)	538
BsnI	GGCC	8 cut(s)	56, 147, 169, 540, 1664, 1909, 2015, 2226
Bso31I	GGTCTC	2 cut(s)	1568, 1989
Bsp119I	TTCGAA	1 cut(s)	120
Bsp1286I	GDGCHC	4 cut(s)	112, 840, 1028, 1459
Bsp143I	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
Bsp1720I	GCTNAGC	2 cut(s)	798, 2121
Bsp19I	CCATGG	1 cut(s)	2670
BspACI	CCGC	6 cut(s)	434, 587, 1321, 1765, 2377, 2855
BspANI	GGCC	8 cut(s)	56, 147, 169, 540, 1664, 1909, 2015, 2226
BspLI	GGNNCC	5 cut(s)	55, 591, 592, 966, 2941
BspMAI	CTGCAG	2 cut(s)	1572, 2026
BspOI	GCTAGC	1 cut(s)	2475
BspPI	GGATC	2 cut(s)	1842, 2908
BspQI	GCTCTTC	2 cut(s)	1359, 1398
BspT104I	TTCGAA	1 cut(s)	120
BspTNI	GGTCTC	2 cut(s)	1568, 1989
BsrBI	CCGCTC	1 cut(s)	2379
BsrDI	GCAATG	2 cut(s)	611, 1109
BsrI	ACTGG	5 cut(s)	788, 888, 1218, 2131, 2814
BssECI	CCNNGG	7 cut(s)	51, 112, 284, 1644, 1665, 2479, 2670
BssMI	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
BssNI	GRCGYC	1 cut(s)	1708
BssSI	CACGAG	1 cut(s)	2370
BssT1I	CCWWGG	2 cut(s)	1644, 2670
Bst2BI	CACGAG	1 cut(s)	2370
Bst2UI	CCWGG	7 cut(s)	71, 285, 822, 1906, 2003, 2480, 2760
Bst4CI	ACNGT	7 cut(s)	319, 457, 526, 859, 922, 2389, 2724
BstACI	GRCGYC	1 cut(s)	1708
BstBAI	YACGTR	1 cut(s)	1673
BstBI	TTCGAA	1 cut(s)	120
BstC8I	GCNNGC	7 cut(s)	538, 1422, 1794, 2473, 2511, 2749, 2769
BstDSI	CCRYGG	1 cut(s)	2670
BstF5I	GGATG	7 cut(s)	542, 721, 748, 838, 1375, 2525, 2666
BstH2I	RGCGCY	2 cut(s)	1867, 2062
BstHHI	GCGC	2 cut(s)	1866, 2061
BstKTI	GATC	8 cut(s)	46, 646, 979, 1464, 1694, 1837, 2048, 2916
BstMAI	GTCTC	8 cut(s)	28, 1242, 1568, 1989, 2070, 2152, 2661, 2785
BstMBI	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
BstNI	CCWGG	7 cut(s)	71, 285, 822, 1906, 2003, 2480, 2760
BstNSI	RCATGY	3 cut(s)	1816, 2656, 2755
BstSCI	CCNGG	8 cut(s)	50, 69, 283, 820, 1904, 2001, 2478, 2758
BstSFI	CTRYAG	6 cut(s)	302, 582, 1554, 1568, 2022, 2315
BstSLI	GKGCMC	1 cut(s)	112
BstV2I	GAAGAC	3 cut(s)	243, 573, 1815
BstX2I	RGATCY	2 cut(s)	1834, 2913
BstYI	RGATCY	2 cut(s)	1834, 2913
Bsu36I	CCTNAGG	1 cut(s)	267
BsuRI	GGCC	8 cut(s)	56, 147, 169, 540, 1664, 1909, 2015, 2226
BtgI	CCRYGG	1 cut(s)	2670
BtsCI	GGATG	7 cut(s)	542, 721, 748, 838, 1375, 2525, 2666
BtsIMutI	CAGTG	7 cut(s)	19, 504, 522, 895, 1225, 2729, 2807
Cac8I	GCNNGC	7 cut(s)	538, 1422, 1794, 2473, 2511, 2749, 2769
CaiI	CAGNNNCTG	3 cut(s)	470, 2030, 2747
CfoI	GCGC	2 cut(s)	1866, 2061
Cfr13I	GGNCC	3 cut(s)	54, 539, 590
CseI	GACGC	2 cut(s)	1352, 1697
Csp6I	GTAC	5 cut(s)	663, 1497, 1892, 2188, 2578
CviAII	CATG	6 cut(s)	149, 704, 1813, 2653, 2671, 2752
CviQI	GTAC	5 cut(s)	663, 1497, 1892, 2188, 2578
DpnI	GATC	8 cut(s)	45, 645, 978, 1463, 1693, 1836, 2047, 2915
DpnII	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
DraIII	CACNNNGTG	1 cut(s)	2603
DriI	GACNNNNNGTC	1 cut(s)	813
EaeI	YGGCCR	2 cut(s)	145, 1662
Eam1105I	GACNNNNNGTC	1 cut(s)	813
Ecl136II	GAGCTC	1 cut(s)	1026
Eco130I	CCWWGG	2 cut(s)	1644, 2670
Eco147I	AGGCCT	1 cut(s)	1909
Eco24I	GRGCYC	3 cut(s)	840, 1028, 1459
Eco31I	GGTCTC	2 cut(s)	1568, 1989
Eco47I	GGWCC	1 cut(s)	590
Eco47III	AGCGCT	1 cut(s)	2060
Eco53kI	GAGCTC	1 cut(s)	1026
Eco57I	CTGAAG	8 cut(s)	141, 480, 990, 1035, 1379, 1416, 1490, 1823
Eco72I	CACGTG	1 cut(s)	1673
Eco81I	CCTNAGG	1 cut(s)	267
EcoICRI	GAGCTC	1 cut(s)	1026
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRII	CCWGG	7 cut(s)	69, 283, 820, 1904, 2001, 2478, 2758
EcoT14I	CCWWGG	2 cut(s)	1644, 2670
EcoT22I	ATGCAT	4 cut(s)	705, 1042, 2296, 2304
EcoT38I	GRGCYC	3 cut(s)	840, 1028, 1459
ErhI	CCWWGG	2 cut(s)	1644, 2670
Esp3I	CGTCTC	1 cut(s)	2785
FaeI	CATG	6 cut(s)	152, 707, 1816, 2656, 2674, 2755
FalI	AAGNNNNNCTT	4 cut(s)	1800, 1832, 2604, 2636
FaqI	GGGAC	3 cut(s)	603, 1322, 1556
FatI	CATG	6 cut(s)	148, 703, 1812, 2652, 2670, 2751
FauI	CCCGC	2 cut(s)	580, 1314
FauNDI	CATATG	1 cut(s)	750
FbaI	TGATCA	1 cut(s)	976
FblI	GTMKAC	1 cut(s)	314
FokI	GGATG	7 cut(s)	529, 728, 755, 845, 1382, 2512, 2653
FriOI	GRGCYC	3 cut(s)	840, 1028, 1459
FspBI	CTAG	9 cut(s)	177, 1700, 1742, 1779, 2079, 2472, 2601, 2777, 2944
GlaI	GCGC	2 cut(s)	1865, 2060
GsaI	CCCAGC	1 cut(s)	677
GsuI	CTGGAG	7 cut(s)	53, 771, 843, 1985, 2577, 2831, 2861
HaeII	RGCGCY	2 cut(s)	1867, 2062
HaeIII	GGCC	8 cut(s)	56, 147, 169, 540, 1664, 1909, 2015, 2226
HapII	CCGG	3 cut(s)	51, 144, 1895
HgaI	GACGC	2 cut(s)	1352, 1697
HhaI	GCGC	2 cut(s)	1866, 2061
Hin1I	GRCGYC	1 cut(s)	1708
Hin1II	CATG	6 cut(s)	152, 707, 1816, 2656, 2674, 2755
Hin6I	GCGC	2 cut(s)	1864, 2059
HinP1I	GCGC	2 cut(s)	1864, 2059
HincII	GTYRAC	2 cut(s)	274, 2908
HindII	GTYRAC	2 cut(s)	274, 2908
HindIII	AAGCTT	4 cut(s)	153, 1656, 1962, 2613
HpaII	CCGG	3 cut(s)	51, 144, 1895
HphI	GGTGA	4 cut(s)	273, 483, 689, 2648
Hpy166II	GTNNAC	6 cut(s)	274, 315, 2639, 2866, 2896, 2908
Hpy8I	GTNNAC	6 cut(s)	274, 315, 2639, 2866, 2896, 2908
HpyCH4III	ACNGT	7 cut(s)	319, 457, 526, 859, 922, 2389, 2724
HpyCH4IV	ACGT	7 cut(s)	311, 1005, 1672, 1729, 2072, 2088, 2827
HpySE526I	ACGT	7 cut(s)	311, 1005, 1672, 1729, 2072, 2088, 2827
Hsp92I	GRCGYC	1 cut(s)	1708
Hsp92II	CATG	6 cut(s)	152, 707, 1816, 2656, 2674, 2755
HspAI	GCGC	2 cut(s)	1864, 2059
KflI	GGGWCCC	1 cut(s)	590
Ksp22I	TGATCA	1 cut(s)	976
Kzo9I	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
LguI	GCTCTTC	2 cut(s)	1359, 1398
LmnI	GCTCC	6 cut(s)	72, 1786, 2812, 2836, 2845, 2939
MaeI	CTAG	9 cut(s)	177, 1700, 1742, 1779, 2079, 2472, 2601, 2777, 2944
MaeII	ACGT	7 cut(s)	311, 1005, 1672, 1729, 2072, 2088, 2827
MaeIII	GTNAC	4 cut(s)	489, 1340, 1823, 2733
MalI	GATC	8 cut(s)	45, 645, 978, 1463, 1693, 1836, 2047, 2915
MbiI	CCGCTC	1 cut(s)	2379
MboI	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
MfeI	CAATTG	1 cut(s)	1194
MflI	RGATCY	2 cut(s)	1834, 2913
MhlI	GDGCHC	4 cut(s)	112, 840, 1028, 1459
MlyI	GAGTC	9 cut(s)	257, 476, 497, 1040, 1244, 1765, 1992, 2516, 2581
MmeI	TCCRAC	2 cut(s)	2337, 2917
Mph1103I	ATGCAT	4 cut(s)	705, 1042, 2296, 2304
MroXI	GAANNNNTTC	2 cut(s)	98, 2679
MslI	CAYNNNNRTG	1 cut(s)	903
MspA1I	CMGCKG	3 cut(s)	1973, 2027, 2171
MspI	CCGG	3 cut(s)	51, 144, 1895
MspR9I	CCNGG	8 cut(s)	52, 71, 285, 822, 1906, 2003, 2480, 2760
MunI	CAATTG	1 cut(s)	1194
Mva1269I	GAATGC	1 cut(s)	538
MvaI	CCWGG	7 cut(s)	71, 285, 822, 1906, 2003, 2480, 2760
NciI	CCSGG	1 cut(s)	52
NcoI	CCATGG	1 cut(s)	2670
NdeI	CATATG	1 cut(s)	750
NdeII	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
NheI	GCTAGC	1 cut(s)	2471
NlaIII	CATG	6 cut(s)	152, 707, 1816, 2656, 2674, 2755
NlaIV	GGNNCC	5 cut(s)	55, 591, 592, 966, 2941
NmeAIII	GCCGAG	2 cut(s)	271, 1690
NmuCI	GTSAC	3 cut(s)	489, 1340, 1823
NsiI	ATGCAT	4 cut(s)	705, 1042, 2296, 2304
NspI	RCATGY	3 cut(s)	1816, 2656, 2755
NspV	TTCGAA	1 cut(s)	120
PceI	AGGCCT	1 cut(s)	1909
PciI	ACATGT	1 cut(s)	2652
PciSI	GCTCTTC	2 cut(s)	1359, 1398
PcsI	WCGNNNNNNNCGW	1 cut(s)	54
PctI	GAATGC	1 cut(s)	538
PdmI	GAANNNNTTC	2 cut(s)	98, 2679
PfoI	TCCNGGA	2 cut(s)	69, 2001
PleI	GAGTC	9 cut(s)	256, 475, 496, 1040, 1243, 1765, 1992, 2516, 2580
PmaCI	CACGTG	1 cut(s)	1673
PmlI	CACGTG	1 cut(s)	1673
PpsI	GAGTC	9 cut(s)	256, 475, 496, 1040, 1243, 1765, 1992, 2516, 2580
Ppu21I	YACGTR	1 cut(s)	1673
PpuMI	RGGWCCY	1 cut(s)	590
PscI	ACATGT	1 cut(s)	2652
PshBI	ATTAAT	1 cut(s)	1380
Psp124BI	GAGCTC	1 cut(s)	1028
Psp1406I	AACGTT	1 cut(s)	2072
Psp5II	RGGWCCY	1 cut(s)	590
Psp6I	CCWGG	7 cut(s)	69, 283, 820, 1904, 2001, 2478, 2758
PspCI	CACGTG	1 cut(s)	1673
PspFI	CCCAGC	1 cut(s)	673
PspGI	CCWGG	7 cut(s)	69, 283, 820, 1904, 2001, 2478, 2758
PspN4I	GGNNCC	5 cut(s)	55, 591, 592, 966, 2941
PspPI	GGNCC	3 cut(s)	54, 539, 590
PspPPI	RGGWCCY	1 cut(s)	590
PstI	CTGCAG	2 cut(s)	1572, 2026
PstNI	CAGNNNCTG	3 cut(s)	470, 2030, 2747
PsuI	RGATCY	2 cut(s)	1834, 2913
PvuII	CAGCTG	3 cut(s)	1973, 2027, 2171
RsaI	GTAC	5 cut(s)	664, 1498, 1893, 2189, 2579
RsaNI	GTAC	5 cut(s)	663, 1497, 1892, 2188, 2578
RseI	CAYNNNNRTG	1 cut(s)	903
SacI	GAGCTC	1 cut(s)	1028
SapI	GCTCTTC	2 cut(s)	1359, 1398
Sau3AI	GATC	8 cut(s)	43, 643, 976, 1461, 1691, 1834, 2045, 2913
Sau96I	GGNCC	3 cut(s)	54, 539, 590
ScaI	AGTACT	2 cut(s)	664, 1498
SchI	GAGTC	9 cut(s)	257, 476, 497, 1040, 1244, 1765, 1992, 2516, 2581
ScrFI	CCNGG	8 cut(s)	52, 71, 285, 822, 1906, 2003, 2480, 2760
SduI	GDGCHC	4 cut(s)	112, 840, 1028, 1459
SfcI	CTRYAG	6 cut(s)	302, 582, 1554, 1568, 2022, 2315
SfuI	TTCGAA	1 cut(s)	120
SinI	GGWCC	1 cut(s)	590
SmiMI	CAYNNNNRTG	1 cut(s)	903
SmlI	CTYRAG	6 cut(s)	561, 1428, 2093, 2217, 2449, 2464
SmoI	CTYRAG	6 cut(s)	561, 1428, 2093, 2217, 2449, 2464
SseBI	AGGCCT	1 cut(s)	1909
SsiI	CCGC	6 cut(s)	434, 587, 1321, 1765, 2377, 2855
SspMI	CTAG	9 cut(s)	177, 1700, 1742, 1779, 2079, 2472, 2601, 2777, 2944
SstI	GAGCTC	1 cut(s)	1028
StuI	AGGCCT	1 cut(s)	1909
StyD4I	CCNGG	8 cut(s)	50, 69, 283, 820, 1904, 2001, 2478, 2758
StyI	CCWWGG	2 cut(s)	1644, 2670
TaaI	ACNGT	7 cut(s)	319, 457, 526, 859, 922, 2389, 2724
TaiI	ACGT	7 cut(s)	314, 1008, 1675, 1732, 2075, 2091, 2830
TaqI	TCGA	6 cut(s)	90, 102, 120, 696, 806, 1996
TatI	WGTACW	2 cut(s)	662, 1496
TauI	GCSGC	1 cut(s)	1768
TscAI	CASTG	7 cut(s)	19, 504, 529, 895, 1225, 2729, 2814
TseFI	GTSAC	3 cut(s)	489, 1340, 1823
Tsp45I	GTSAC	3 cut(s)	489, 1340, 1823
TspGWI	ACGGA	3 cut(s)	832, 1811, 2565
TspRI	CASTG	7 cut(s)	19, 504, 529, 895, 1225, 2729, 2814
VpaK11BI	GGWCC	1 cut(s)	590
VspI	ATTAAT	1 cut(s)	1380
XapI	RAATTY	4 cut(s)	97, 601, 709, 1945
XbaI	TCTAGA	1 cut(s)	2078
XceI	RCATGY	3 cut(s)	1816, 2656, 2755
XmiI	GTMKAC	1 cut(s)	314
XmnI	GAANNNNTTC	2 cut(s)	98, 2679
XspI	CTAG	9 cut(s)	177, 1700, 1742, 1779, 2079, 2472, 2601, 2777, 2944
ZrmI	AGTACT	2 cut(s)	664, 1498
Zsp2I	ATGCAT	4 cut(s)	705, 1042, 2296, 2304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.