Rorug02G0354600

Golgin candidate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004830
Physical Location & Seq
Forward (+)
44602250 .. 44605267
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0354600.1

Sequence Viewer

Length: 1233 bp
ATGATGAGTGGTTGCAAGAGAACGGCACTAATGCCGCGGCAATCGTCGTACAATTCCCTCTACGTCAACCCCCTCAACGACCCAAAACACAACCGCAGTTCCAGCGAAGGCGACGATCATTCCAAACCAAACCAGTTGGCTTTGGCTTCGAACGACGAAGACAAAGAGAACGCAACGCCCAACAGCAGCAGCAACGACAAGGAGAACTTGCTGGCGAAGAAGCCACTGAAGCAGAATCAACTGAACAAGTCTTTCTCCACCGGCTGCGGCGGCGGCGGTGGTGGGGCCTTGAAGCCGTCGTCGCTTCAGTTCTGTATGCAGATGAGTGAGCCGGAGAAGGAGCTCTTTGGTGGGTCCAAGATGTGGGACCAGCCCAGTGTGACGTCGGACAACTCTAGTTCTTTGAAGATATGGGACTACTCGGATTCTGAGGCTGCTCCTGCTTCTTCTTGGTCCACTCTGCCTAATAAGTCTTTGCTGTGTAGGCCATTACCTATGGATGTAGGAAGGTGCACTTGTGTTATAGTGAAGGAAAAATCTCCAGATGGGGGCTCTCTGTATTCTCTCTATACCAATGAAGGCAAGGGAAGGCAGGACAGGAAACTAGCTGTTGCACACCATAAGCGGCGTAATGGAAAGTCTGAGTTAACTATTGCTCAAAATACGAAGGGAATATTGTCACATTCAGATGATAGTTTTATTGGGATGGTAAATGCTAATCTCATGGGTTCAAAGTACCACATATGGGATCAGGGGAAGCAACCAAATTGCTTGAGTAAACAGTCTAAATCACTTCTTGCTGCTGTATCATTCATACCCACAATTTCAACTTGGACAGGAAGTTACAGACGTATAAGGGCATATGTACCCAAGCACCAATCGATGCAGTTGAAGAATACTGCTCAGGTACAACACATTAAGGGACTGCCAACAGACTGGGAGGCGAAAATGGACAAAATCCATCAGCTATACTCAAGGGTTCCACTTTACAATAAAATTTCAAAGCAATACGAGTTGGACTTCAGAGATAGGGGAAGAGCTGGGCTTAGAATCCAAAGTTCAGTTAAAAATTTCCAGCTGACTCTGGAGGAGAACGGAAAGCAGACAATCCTGCAGTTAGGAAGGGTTGGGAAGTCAAAATATGTGATGGATTACAGATATCCTTTGACAGGCTACCAAGCATTTTGCATTTGTCTGGCTTCTATTGATTCAAAGCTCTGTTGCTCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMF_DNA_bd TMF_TATA_bd

Protein Analysis

410

Amino Acids

45.61

Weight (kDa)

9.39

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 160 - 404 5.2e-49 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 363
AccII CGCG 1 cut(s) 37
AciI CCGC 8 cut(s) 35, 37, 94, 267, 270, 273, 276, 625
AclWI GGATC 1 cut(s) 756
AcsI RAATTY 2 cut(s) 996, 1069
AcuI CTGAAG 3 cut(s) 248, 290, 1006
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 4 cut(s) 50, 737, 867, 909
AfiI CCNNNNNNNGG 4 cut(s) 363, 548, 745, 1169
AgsI TTSAA 7 cut(s) 292, 406, 732, 828, 892, 1002, 1212
AluBI AGCT 6 cut(s) 343, 608, 967, 1040, 1078, 1216
AluI AGCT 6 cut(s) 343, 608, 967, 1040, 1078, 1216
Alw21I GWGCWC 2 cut(s) 345, 515
Alw44I GTGCAC 1 cut(s) 511
AlwI GGATC 1 cut(s) 756
AoxI GGCC 2 cut(s) 285, 485
ApaLI GTGCAC 1 cut(s) 511
ApeKI GCWGC 5 cut(s) 186, 189, 264, 434, 800
ApoI RAATTY 2 cut(s) 996, 1069
AspS9I GGNCC 4 cut(s) 285, 354, 367, 453
AsuII TTCGAA 1 cut(s) 149
AvaII GGWCC 3 cut(s) 354, 367, 453
BaeGI GKGCMC 1 cut(s) 515
BanII GRGCYC 2 cut(s) 345, 554
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 2 cut(s) 345, 515
BbvI GCAGC 5 cut(s) 198, 201, 251, 421, 787
BccI CCATC 4 cut(s) 539, 700, 969, 1141
BceAI ACGGC 2 cut(s) 39, 280
BfaI CTAG 2 cut(s) 396, 605
BfmI CTRYAG 1 cut(s) 1112
Bme18I GGWCC 3 cut(s) 354, 367, 453
BmgT120I GGNCC 4 cut(s) 285, 354, 367, 453
BmiI GGNNCC 4 cut(s) 286, 355, 368, 981
BmrI ACTGGG 2 cut(s) 369, 946
BmsI GCATC 1 cut(s) 873
BmuI ACTGGG 2 cut(s) 369, 946
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 2 cut(s) 525, 1106
Bpu10I CCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 149
BpuEI CTTGAG 2 cut(s) 793, 958
Bsa29I ATCGAT 1 cut(s) 881
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 4 cut(s) 363, 548, 745, 1169
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 133, 375, 941
BseCI ATCGAT 1 cut(s) 881
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 2 cut(s) 505, 711
BseLI CCNNNNNNNGG 4 cut(s) 363, 548, 745, 1169
BseMII CTCAG 3 cut(s) 420, 633, 917
BseNI ACTGG 3 cut(s) 133, 375, 941
BseRI GAGGAG 1 cut(s) 1103
BseSI GKGCMC 1 cut(s) 515
BseXI GCAGC 5 cut(s) 198, 201, 251, 421, 787
BseYI CCCAGC 1 cut(s) 1040
Bsh1236I CGCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 287, 487
BshVI ATCGAT 1 cut(s) 881
BsiHKAI GWGCWC 2 cut(s) 345, 515
BsiSI CCGG 2 cut(s) 261, 332
BslFI GGGAC 3 cut(s) 380, 428, 936
BslI CCNNNNNNNGG 4 cut(s) 363, 548, 745, 1169
BsmFI GGGAC 3 cut(s) 380, 428, 936
BsnI GGCC 2 cut(s) 287, 487
Bsp119I TTCGAA 1 cut(s) 149
Bsp1286I GDGCHC 3 cut(s) 345, 515, 554
Bsp143I GATC 2 cut(s) 115, 748
BspACI CCGC 8 cut(s) 35, 37, 94, 267, 270, 273, 276, 625
BspANI GGCC 2 cut(s) 287, 487
BspCNI CTCAG 3 cut(s) 421, 634, 916
BspDI ATCGAT 1 cut(s) 881
BspFNI CGCG 1 cut(s) 37
BspLI GGNNCC 4 cut(s) 286, 355, 368, 981
BspMAI CTGCAG 1 cut(s) 1116
BspPI GGATC 1 cut(s) 756
BspQI GCTCTTC 1 cut(s) 1030
BspT104I TTCGAA 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 3 cut(s) 133, 375, 941
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 2 cut(s) 115, 748
BssNI GRCGYC 1 cut(s) 383
Bst4CI ACNGT 1 cut(s) 783
Bst6I CTCTTC 1 cut(s) 1030
BstACI GRCGYC 1 cut(s) 383
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 5 cut(s) 429, 642, 903, 1046, 1225
BstDSI CCRYGG 1 cut(s) 35
BstENI CCTNNNNNAGG 1 cut(s) 1167
BstF5I GGATG 2 cut(s) 505, 711
BstFNI CGCG 1 cut(s) 37
BstKTI GATC 2 cut(s) 118, 751
BstMBI GATC 2 cut(s) 115, 748
BstMWI GCNNNNNNNGC 7 cut(s) 102, 229, 270, 273, 301, 440, 484
BstSFI CTRYAG 1 cut(s) 1112
BstSLI GKGCMC 1 cut(s) 515
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 5 cut(s) 198, 201, 251, 421, 787
BstV2I GAAGAC 1 cut(s) 165
BstXI CCANNNNNNTGG 1 cut(s) 936
Bsu15I ATCGAT 1 cut(s) 881
BsuRI GGCC 2 cut(s) 287, 487
BsuTUI ATCGAT 1 cut(s) 881
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 2 cut(s) 505, 711
BtsIMutI CAGTG 2 cut(s) 224, 382
Cac8I GCNNGC 1 cut(s) 213
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 4 cut(s) 285, 354, 367, 453
Cfr42I CCGCGG 1 cut(s) 38
ClaI ATCGAT 1 cut(s) 881
Csp6I GTAC 4 cut(s) 49, 736, 866, 908
CviAII CATG 1 cut(s) 724
CviQI GTAC 4 cut(s) 49, 736, 866, 908
DdeI CTNAG 5 cut(s) 429, 642, 903, 1046, 1225
DpnI GATC 2 cut(s) 117, 750
DpnII GATC 2 cut(s) 115, 748
Eam1104I CTCTTC 1 cut(s) 1030
EarI CTCTTC 1 cut(s) 1030
Ecl136II GAGCTC 1 cut(s) 343
Eco24I GRGCYC 2 cut(s) 345, 554
Eco32I GATATC 1 cut(s) 1160
Eco47I GGWCC 3 cut(s) 354, 367, 453
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 3 cut(s) 248, 290, 1006
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 1167
EcoO109I RGGNCCY 1 cut(s) 285
EcoRV GATATC 1 cut(s) 1160
EcoT38I GRGCYC 2 cut(s) 345, 554
FaeI CATG 1 cut(s) 727
FalI AAGNNNNNCTT 6 cut(s) 191, 223, 329, 361, 499, 531
FaqI GGGAC 3 cut(s) 380, 428, 936
FatI CATG 1 cut(s) 723
FauNDI CATATG 2 cut(s) 743, 862
FokI GGATG 2 cut(s) 512, 718
FriOI GRGCYC 2 cut(s) 345, 554
FspBI CTAG 2 cut(s) 396, 605
GsaI CCCAGC 1 cut(s) 1044
GsuI CTGGAG 2 cut(s) 525, 1106
HaeIII GGCC 2 cut(s) 287, 487
HapII CCGG 2 cut(s) 261, 332
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 1 cut(s) 727
HincII GTYRAC 2 cut(s) 67, 648
HindII GTYRAC 2 cut(s) 67, 648
HinfI GANTC 5 cut(s) 235, 425, 1050, 1081, 1208
HpaI GTTAAC 1 cut(s) 648
HpaII CCGG 2 cut(s) 261, 332
Hpy166II GTNNAC 5 cut(s) 67, 456, 513, 648, 779
Hpy188I TCNGA 6 cut(s) 388, 424, 430, 643, 688, 1025
Hpy188III TCNNGA 2 cut(s) 542, 1085
Hpy8I GTNNAC 5 cut(s) 67, 456, 513, 648, 779
Hpy99I CGWCG 6 cut(s) 49, 116, 158, 301, 304, 388
HpyAV CCTTC 8 cut(s) 101, 331, 501, 523, 572, 582, 661, 1116
HpyCH4III ACNGT 1 cut(s) 783
HpyCH4IV ACGT 3 cut(s) 63, 383, 850
HpyCH4V TGCA 7 cut(s) 15, 319, 513, 614, 886, 1114, 1188
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 229, 270, 273, 301, 440, 484
HpyF3I CTNAG 5 cut(s) 429, 642, 903, 1046, 1225
HpySE526I ACGT 3 cut(s) 63, 383, 850
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 1 cut(s) 727
KspAI GTTAAC 1 cut(s) 648
KspI CCGCGG 1 cut(s) 38
Kzo9I GATC 2 cut(s) 115, 748
LguI GCTCTTC 1 cut(s) 1030
LmnI GCTCC 2 cut(s) 340, 442
Lsp1109I GCAGC 5 cut(s) 198, 201, 251, 421, 787
LweI GCATC 1 cut(s) 873
MaeI CTAG 2 cut(s) 396, 605
MaeII ACGT 3 cut(s) 63, 383, 850
MaeIII GTNAC 3 cut(s) 379, 678, 842
MalI GATC 2 cut(s) 117, 750
MboI GATC 2 cut(s) 115, 748
MboII GAAGA 6 cut(s) 170, 229, 418, 438, 904, 1047
MhlI GDGCHC 3 cut(s) 345, 515, 554
MluCI AATT 5 cut(s) 52, 766, 822, 996, 1069
MlyI GAGTC 1 cut(s) 1075
MmeI TCCRAC 2 cut(s) 366, 996
MnlI CCTC 5 cut(s) 68, 83, 424, 934, 1081
MseI TTAA 3 cut(s) 647, 918, 1065
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 2 cut(s) 37, 1078
MspI CCGG 2 cut(s) 261, 332
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 7 cut(s) 102, 229, 270, 273, 301, 440, 484
NdeI CATATG 2 cut(s) 743, 862
NdeII GATC 2 cut(s) 115, 748
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 4 cut(s) 286, 355, 368, 981
NmuCI GTSAC 2 cut(s) 379, 678
NspV TTCGAA 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 1030
PfeI GAWTC 4 cut(s) 235, 425, 1050, 1208
PflMI CCANNNNNTGG 1 cut(s) 363
PleI GAGTC 1 cut(s) 1075
PpsI GAGTC 1 cut(s) 1075
Psp124BI GAGCTC 1 cut(s) 345
PspFI CCCAGC 1 cut(s) 1040
PspN4I GGNNCC 4 cut(s) 286, 355, 368, 981
PspPI GGNCC 4 cut(s) 285, 354, 367, 453
PstI CTGCAG 1 cut(s) 1116
PvuII CAGCTG 1 cut(s) 1078
RsaI GTAC 4 cut(s) 50, 737, 867, 909
RsaNI GTAC 4 cut(s) 49, 736, 866, 908
RseI CAYNNNNRTG 1 cut(s) 687
SacI GAGCTC 1 cut(s) 345
SacII CCGCGG 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 1030
SaqAI TTAA 3 cut(s) 647, 918, 1065
Sau3AI GATC 2 cut(s) 115, 748
Sau96I GGNCC 4 cut(s) 285, 354, 367, 453
SchI GAGTC 1 cut(s) 1075
SduI GDGCHC 3 cut(s) 345, 515, 554
SfaNI GCATC 1 cut(s) 873
SfcI CTRYAG 1 cut(s) 1112
Sfr303I CCGCGG 1 cut(s) 38
SfuI TTCGAA 1 cut(s) 149
SgrBI CCGCGG 1 cut(s) 38
SinI GGWCC 3 cut(s) 354, 367, 453
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 772, 973
SmoI CTYRAG 2 cut(s) 772, 973
Sse9I AATT 5 cut(s) 52, 766, 822, 996, 1069
SsiI CCGC 8 cut(s) 35, 37, 94, 267, 270, 273, 276, 625
SspI AATATT 1 cut(s) 675
SspMI CTAG 2 cut(s) 396, 605
SstI GAGCTC 1 cut(s) 345
TaaI ACNGT 1 cut(s) 783
TaiI ACGT 3 cut(s) 66, 386, 853
TaqI TCGA 2 cut(s) 149, 881
TasI AATT 5 cut(s) 52, 766, 822, 996, 1069
TauI GCSGC 6 cut(s) 37, 40, 270, 273, 276, 628
TfiI GAWTC 4 cut(s) 235, 425, 1050, 1208
Tru1I TTAA 3 cut(s) 647, 918, 1065
Tru9I TTAA 3 cut(s) 647, 918, 1065
TscAI CASTG 2 cut(s) 231, 382
TseFI GTSAC 2 cut(s) 379, 678
TseI GCWGC 5 cut(s) 186, 189, 264, 434, 800
Tsp45I GTSAC 2 cut(s) 379, 678
TspDTI ATGAA 2 cut(s) 591, 802
TspGWI ACGGA 1 cut(s) 1110
TspRI CASTG 2 cut(s) 231, 382
Van91I CCANNNNNTGG 1 cut(s) 363
VneI GTGCAC 1 cut(s) 511
VpaK11BI GGWCC 3 cut(s) 354, 367, 453
XagI CCTNNNNNAGG 1 cut(s) 1167
XapI RAATTY 2 cut(s) 996, 1069
XspI CTAG 2 cut(s) 396, 605
ZraI GACGTC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.