MD09G1214900.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005735
Physical Location & Seq
Forward (+)
21182019 .. 21187963
5945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1214900.v1.1.491

Sequence Viewer

Length: 2472 bp
ATGGCTTCGGTGGGCGAGGAGGATGTGGATTATGACAGTGATCCGGAGGAAGCGAAGCGATCTCTGGCGATGCGGAGGAGGGCAGCGGCGAGCGATGATGAGGAAGGTGAAGAGGAGGTGAGGGAGAAGCCAAGGGTGGATCCAAGGGCCGTGGTTCACTCCGATGAATCGGATGATCAGGGTGGGGCGGCTGAGTACGATGATGACGAAGAACTAGAGGATGAAGAAGAAGAGATTGATGAAGAAGAAGAGGTTGAGGAGGAAGTTGTGAGAGAAGAGGACTTGGAAGGATATCGGGATAGCGGCCGTGAAGGCAAGACGGGTGGCAGGGTTGAGGTTTTGGCTGTTACGGAAATGGAAGGTGATGGGCAGAGGTCTGTGGAAGGGTCCGTGGATGGGAATGGGGGTAATCAGGTCGAAGAAGGGGAGGAGAAGAAGGAGAATGAGCCATTTGCAGTGCCGACAGCGGGTGCATTTTACATGCATGATGACCGTTTCAGGGACAATTCTGTTGGTCGGCACAGGCGAACACATGGTGGAAGGAAGCTGTGGGAGTCCAAAGATGAAAGGAAATGGGGGCACGATAAGTTTGAAGAGATTACTTTGCAGGAAAGGCACTACGAAGAGGGAAAGAAACCTTTGAAGGGTCCTTATGTAGGTCGCGGAAGAAACCGAGGTATTGATCGTGGGAGTACCAGAGGAAACAGGTCTAATGAATATGACAATAATAATCTACAGGTACCCAAGGGTGTGAGAGGGAGAGGGCCCAGGAGGTATGAACCGGCTTTTAGGAATAACAGACAGGCACAGCCAACACAAAACAAACAGTCTGGAAAGCCTGCCGAGAAAAACGTACAAAGTAATTCAGAGAGAACATTTACTCCCAACTCAAATGCACAATCTGACCAAGTTTTTGCTAGGAAAAATGTCTTTGCCTCAGGCTTGAATTCAGCTTCTCCTCCTTTTTACCCATCAGGTTCTTCCAGTTCCAATAAAGACATCACTCTTTCCCAAAAGCGGGATGTACAGGCTGGGAGTGGTAAGAGAAATGTTCGGTCTTCTGTCATGCGGGAGAGTTTCAATGTGCCCCAAACCAATGCATTGGTGCGAGGGAAGAATATAGCTGAATCTGTTGATATGGACAAGCTTTATATTAATGACTCTACCAACCCAGCTACTGGGAAGACTTTGACTAACATACAAATGTCACCTTCTGGGTCATTTGTCAATACCACTCAAGCTCCTCAGTCTAGGGTTCAAGGAAGGGGCGTTATCCATGGACAGATGAGTTATCAACCGGTGTCTCAGAACCAAATGACAAGAGCTTCTCCATCAACACATCTCCATGCTGTTCGGCGCAATCCTGCTCAAAATCGATCTCAACTGGCTCTGCAAGCTCCTGCTCAGCAGATGGGAGAACGCCCTGGAAGTGAATCTCAAGCTTCCTCTCCGCCCAAAACAACCATGTCATCAAATTCCTTCGAATCGGGAGAAGTTGAATTACCTTCAGATTCAAGTAAATCTAAAGGTGCCCTGGTTGCGAAGGGAAAAGGCAGTGTTCAGGGTAGTGGAAGGGGCACTCTTCCGTATGGCGGAGCTCCGGTTATGGGACCCACTGGGAGTATGGATGTTAATCATAAAGATATTAACTTTCCTGGTACTCCAGCCTTTTTACCTGTCATGCAATTTGGGGGCCAGCATACTGGGGGTATGGGAGTTCCTGCTGTTGGCATGGCCTTTCCTGGATATGTTGCTCAGCCACAACTTGGCGGTTTAGGAAATTCGGGCGAAATGACATGGTTACCTGTATTGGCGGGTGCTGCAGGAGCACTGGGGGCAACATATTGCCCTTCCTATATTACTGTTGATGGTTCTTATCATGCTCGCCCATTAGGACAAACATCTTCTGTGGGTTCCTTGAGCAAAGAGAATAATCCAAATAAAACCAGTAACGAATGGAAGCCTTCACAAAAGCCTGAGATTGTGGGAGATGAATATGGGCAACGACAAAACAAGCCTCGCAGATACTCACAGATGAATGTGGCCAGTGAAGTACTTCAAAATAACATGGAGCTTTCCTTCGTTCAGCCCTTTGTGATCTTCAGCTTATGTTCTGGGGCACTTTGGGCCAATGTGTTCCAAGATATCTTAGTTATGTATGACAAACACCTGCTCATGCTGTACTCAACCTACGATCAACATTTGCGGCTTGACGGGTGGCATTGCTTGTTTACATTTTCGGTTTTCTTGTGGTTTGGTTTTTTTATCGAAAAGCTCGTGAGAAATGAAGACGAGCCTCCTCATAAACTACTATATAAAGAAAATGGAAAAAAAAGGAGTACAATGAACTGTTGTGCAGCAGCAGCAGTTCCGTCGGTCGGATTTTCAAAAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAGATATAGTTGCATTGGTTTTTTTGTTTATTTATATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Btz

Protein Analysis

824

Amino Acids

91.05

Weight (kDa)

5.7

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Btz PF09405 138 - 211 3.1e-18 CASC3/Barentsz eIF4AIII binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2178
Acc36I ACCTGC 1 cut(s) 2178
Acc65I GGTACC 1 cut(s) 739
AccB1I GGYRCC 2 cut(s) 739, 1529
AccB7I CCANNNNNTGG 2 cut(s) 1178, 1766
AccII CGCG 1 cut(s) 663
AccIII TCCGGA 1 cut(s) 43
AclWI GGATC 3 cut(s) 35, 134, 147
AcoI YGGCCR 2 cut(s) 304, 2043
AcsI RAATTY 3 cut(s) 946, 1474, 1780
AcuI CTGAAG 2 cut(s) 1491, 2086
AdeI CACNNNGTG 1 cut(s) 536
AfaI GTAC 9 cut(s) 197, 694, 741, 855, 1026, 1660, 2055, 2183, 2341
AgeI ACCGGT 1 cut(s) 1297
AgsI TTSAA 9 cut(s) 593, 643, 946, 1081, 1259, 1499, 1515, 2060, 2388
AjnI CCWGG 5 cut(s) 767, 1423, 1533, 1654, 1741
AloI GAACNNNNNNTCC 2 cut(s) 865, 897
Alw21I GWGCWC 2 cut(s) 1600, 1831
Alw26I GTCTC 1 cut(s) 1308
AlwI GGATC 3 cut(s) 35, 134, 147
AlwNI CAGNNNCTG 1 cut(s) 1178
Aor13HI TCCGGA 1 cut(s) 43
AoxI GGCC 7 cut(s) 147, 304, 764, 1693, 1734, 2043, 2127
ApaI GGGCCC 1 cut(s) 768
ApeKI GCWGC 5 cut(s) 83, 1820, 2357, 2360, 2363
ApoI RAATTY 3 cut(s) 946, 1474, 1780
ArsI GACNNNNNNTTYG 2 cut(s) 896, 928
AseI ATTAAT 1 cut(s) 1155
AsiGI ACCGGT 1 cut(s) 1297
Asp700I GAANNNNTTC 1 cut(s) 2055
Asp718I GGTACC 1 cut(s) 739
AspLEI GCGC 1 cut(s) 1359
AspS9I GGNCC 8 cut(s) 147, 387, 647, 764, 765, 1610, 1693, 2127
AsuHPI GGTGA 4 cut(s) 119, 130, 374, 1200
AsuII TTCGAA 1 cut(s) 1482
AvaII GGWCC 3 cut(s) 387, 647, 1610
AxyI CCTNAGG 1 cut(s) 937
BaeGI GKGCMC 6 cut(s) 582, 768, 1089, 1534, 1580, 2122
BalI TGGCCA 1 cut(s) 2045
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 2 cut(s) 739, 1529
BanII GRGCYC 2 cut(s) 768, 1600
BarI GAAGNNNNNNTAC 2 cut(s) 1485, 1517
BauI CACGAG 1 cut(s) 2276
BbsI GAAGAC 3 cut(s) 1050, 1190, 2295
Bbv12I GWGCWC 2 cut(s) 1600, 1831
BbvI GCAGC 5 cut(s) 95, 1807, 2369, 2372, 2375
BccI CCATC 6 cut(s) 359, 389, 979, 1339, 1405, 1862
BceAI ACGGC 2 cut(s) 134, 291
BcgI CGANNNNNNTGC 2 cut(s) 2355, 2389
BciT130I CCWGG 5 cut(s) 769, 1425, 1535, 1656, 1743
BclI TGATCA 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 1308
BfaI CTAG 3 cut(s) 215, 918, 1251
BfmI CTRYAG 2 cut(s) 734, 1821
BfuAI ACCTGC 1 cut(s) 2178
BglI GCCNNNNNGGC 1 cut(s) 312
BisI GCNGC 9 cut(s) 84, 87, 189, 304, 1821, 2207, 2358, 2361, 2364
BlpI GCTNAGC 2 cut(s) 1404, 1755
BlsI GCNGC 9 cut(s) 85, 88, 190, 305, 1822, 2208, 2359, 2362, 2365
BmcAI AGTACT 1 cut(s) 2055
Bme1390I CCNGG 5 cut(s) 769, 1425, 1535, 1656, 1743
Bme18I GGWCC 3 cut(s) 387, 647, 1610
BmgT120I GGNCC 8 cut(s) 147, 387, 647, 764, 765, 1610, 1693, 2127
BmrFI CCNGG 5 cut(s) 769, 1425, 1535, 1656, 1743
BmrI ACTGGG 4 cut(s) 1188, 1626, 1713, 1841
BmsI GCATC 1 cut(s) 60
BmuI ACTGGG 4 cut(s) 1188, 1626, 1713, 1841
BpiI GAAGAC 3 cut(s) 1050, 1190, 2295
BpmI CTGGAG 1 cut(s) 1647
Bpu1102I GCTNAGC 2 cut(s) 1404, 1755
Bpu14I TTCGAA 1 cut(s) 1482
BpuEI CTTGAG 3 cut(s) 1221, 1422, 1939
Bsa29I ATCGAT 1 cut(s) 1375
BsaBI GATNNNNATC 1 cut(s) 1632
BsaWI WCCGGW 3 cut(s) 43, 1297, 1600
BsaXI ACNNNNNCTCC 6 cut(s) 865, 895, 1225, 1255, 1587, 1617
Bse118I RCCGGY 2 cut(s) 781, 1297
Bse1I ACTGG 8 cut(s) 984, 1183, 1389, 1621, 1708, 1836, 1947, 2046
Bse21I CCTNAGG 1 cut(s) 937
Bse3DI GCAATG 1 cut(s) 2221
Bse8I GATNNNNATC 1 cut(s) 1632
BseAI TCCGGA 1 cut(s) 43
BseBI CCWGG 5 cut(s) 769, 1425, 1535, 1656, 1743
BseCI ATCGAT 1 cut(s) 1375
BseGI GGATG 6 cut(s) 28, 178, 226, 400, 1027, 1633
BseJI GATNNNNATC 1 cut(s) 1632
BseMI GCAATG 1 cut(s) 2221
BseMII CTCAG 7 cut(s) 183, 951, 1259, 1319, 1418, 1769, 1968
BseNI ACTGG 8 cut(s) 984, 1183, 1389, 1621, 1708, 1836, 1947, 2046
BseRI GAGGAG 8 cut(s) 32, 91, 128, 272, 443, 948, 1233, 2289
BseSI GKGCMC 6 cut(s) 582, 768, 1089, 1534, 1580, 2122
BseX3I CGGCCG 1 cut(s) 304
BseXI GCAGC 5 cut(s) 95, 1807, 2369, 2372, 2375
BseYI CCCAGC 2 cut(s) 1031, 1171
BsgI GTGCAG 1 cut(s) 2376
Bsh1236I CGCG 1 cut(s) 663
Bsh1285I CGRYCG 2 cut(s) 307, 2379
BshFI GGCC 7 cut(s) 149, 306, 766, 1695, 1736, 2045, 2129
BshNI GGYRCC 2 cut(s) 739, 1529
BshTI ACCGGT 1 cut(s) 1297
BshVI ATCGAT 1 cut(s) 1375
BsiEI CGRYCG 2 cut(s) 307, 2379
BsiHKAI GWGCWC 2 cut(s) 1600, 1831
BsiSI CCGG 4 cut(s) 44, 782, 1298, 1601
BslFI GGGAC 2 cut(s) 515, 1623
BsmAI GTCTC 1 cut(s) 1308
BsmFI GGGAC 2 cut(s) 515, 1623
BsnI GGCC 7 cut(s) 149, 306, 766, 1695, 1736, 2045, 2129
Bsp119I TTCGAA 1 cut(s) 1482
Bsp120I GGGCCC 1 cut(s) 764
Bsp1286I GDGCHC 8 cut(s) 582, 768, 1089, 1534, 1580, 1600, 1831, 2122
Bsp13I TCCGGA 1 cut(s) 43
Bsp1407I TGTACA 1 cut(s) 1024
Bsp143I GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
Bsp1720I GCTNAGC 2 cut(s) 1404, 1755
Bsp19I CCATGG 1 cut(s) 1276
BspANI GGCC 7 cut(s) 149, 306, 766, 1695, 1736, 2045, 2129
BspCNI CTCAG 7 cut(s) 184, 950, 1258, 1318, 1417, 1768, 1969
BspDI ATCGAT 1 cut(s) 1375
BspEI TCCGGA 1 cut(s) 43
BspFNI CGCG 1 cut(s) 663
BspMAI CTGCAG 1 cut(s) 1825
BspMI ACCTGC 1 cut(s) 2178
BspPI GGATC 3 cut(s) 35, 134, 147
BspT104I TTCGAA 1 cut(s) 1482
BspT107I GGYRCC 2 cut(s) 739, 1529
BsrDI GCAATG 1 cut(s) 2221
BsrFI RCCGGY 2 cut(s) 781, 1297
BsrGI TGTACA 1 cut(s) 1024
BsrI ACTGG 8 cut(s) 984, 1183, 1389, 1621, 1708, 1836, 1947, 2046
BssAI RCCGGY 2 cut(s) 781, 1297
BssMI GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
BssSI CACGAG 1 cut(s) 2276
BssT1I CCWWGG 4 cut(s) 131, 143, 744, 1276
Bst2BI CACGAG 1 cut(s) 2276
Bst2UI CCWGG 5 cut(s) 769, 1425, 1535, 1656, 1743
Bst4CI ACNGT 5 cut(s) 38, 494, 828, 1864, 2351
Bst6I CTCTTC 7 cut(s) 105, 225, 243, 270, 588, 618, 1587
BstAUI TGTACA 1 cut(s) 1024
BstBI TTCGAA 1 cut(s) 1482
BstC8I GCNNGC 5 cut(s) 91, 840, 1395, 1697, 1885
BstDEI CTNAG 8 cut(s) 192, 937, 1245, 1305, 1404, 1755, 1977, 2149
BstDSI CCRYGG 3 cut(s) 150, 390, 1276
BstEII GGTNACC 1 cut(s) 1800
BstENI CCTNNNNNAGG 1 cut(s) 654
BstF5I GGATG 6 cut(s) 28, 178, 226, 400, 1027, 1633
BstFNI CGCG 1 cut(s) 663
BstHHI GCGC 1 cut(s) 1359
BstKTI GATC 8 cut(s) 43, 62, 142, 178, 685, 1379, 2100, 2197
BstMAI GTCTC 1 cut(s) 1308
BstMBI GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
BstMCI CGRYCG 2 cut(s) 307, 2379
BstMWI GCNNNNNNNGC 9 cut(s) 312, 613, 1394, 1538, 1820, 1826, 1835, 2126, 2363
BstNI CCWGG 5 cut(s) 769, 1425, 1535, 1656, 1743
BstNSI RCATGY 1 cut(s) 484
BstPI GGTNACC 1 cut(s) 1800
BstSCI CCNGG 5 cut(s) 767, 1423, 1533, 1654, 1741
BstSFI CTRYAG 2 cut(s) 734, 1821
BstSLI GKGCMC 6 cut(s) 582, 768, 1089, 1534, 1580, 2122
BstUI CGCG 1 cut(s) 663
BstV1I GCAGC 5 cut(s) 95, 1807, 2369, 2372, 2375
BstV2I GAAGAC 3 cut(s) 1050, 1190, 2295
BstX2I RGATCY 1 cut(s) 139
BstXI CCANNNNNNTGG 2 cut(s) 1102, 1703
BstYI RGATCY 1 cut(s) 139
BstZI CGGCCG 1 cut(s) 304
Bsu15I ATCGAT 1 cut(s) 1375
Bsu36I CCTNAGG 1 cut(s) 937
BsuRI GGCC 7 cut(s) 149, 306, 766, 1695, 1736, 2045, 2129
BsuTUI ATCGAT 1 cut(s) 1375
BtgI CCRYGG 3 cut(s) 150, 390, 1276
BtgZI GCGATG 2 cut(s) 83, 108
BtsCI GGATG 6 cut(s) 28, 178, 226, 400, 1027, 1633
BtsI GCAGTG 2 cut(s) 462, 1561
BtsIMutI CAGTG 6 cut(s) 43, 462, 1561, 1614, 1829, 2053
BveI ACCTGC 1 cut(s) 2178
Cac8I GCNNGC 5 cut(s) 91, 840, 1395, 1697, 1885
CaiI CAGNNNCTG 1 cut(s) 1178
CfoI GCGC 1 cut(s) 1359
Cfr10I RCCGGY 2 cut(s) 781, 1297
Cfr13I GGNCC 8 cut(s) 147, 387, 647, 764, 765, 1610, 1693, 2127
ClaI ATCGAT 1 cut(s) 1375
Csp6I GTAC 9 cut(s) 196, 693, 740, 854, 1025, 1659, 2054, 2182, 2340
CspAI ACCGGT 1 cut(s) 1297
CspCI CAANNNNNGTGG 4 cut(s) 132, 167, 304, 339
CviQI GTAC 9 cut(s) 196, 693, 740, 854, 1025, 1659, 2054, 2182, 2340
DdeI CTNAG 8 cut(s) 192, 937, 1245, 1305, 1404, 1755, 1977, 2149
DpnI GATC 8 cut(s) 42, 61, 141, 177, 684, 1378, 2099, 2196
DpnII GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
DraIII CACNNNGTG 1 cut(s) 536
EaeI YGGCCR 2 cut(s) 304, 2043
EagI CGGCCG 1 cut(s) 304
Eam1104I CTCTTC 7 cut(s) 105, 225, 243, 270, 588, 618, 1587
EarI CTCTTC 7 cut(s) 105, 225, 243, 270, 588, 618, 1587
EciI GGCGGA 2 cut(s) 1440, 1608
Ecl136II GAGCTC 1 cut(s) 1598
EclXI CGGCCG 1 cut(s) 304
Eco130I CCWWGG 4 cut(s) 131, 143, 744, 1276
Eco24I GRGCYC 2 cut(s) 768, 1600
Eco32I GATATC 2 cut(s) 293, 2146
Eco47I GGWCC 3 cut(s) 387, 647, 1610
Eco52I CGGCCG 1 cut(s) 304
Eco53kI GAGCTC 1 cut(s) 1598
Eco57I CTGAAG 2 cut(s) 1491, 2086
Eco81I CCTNAGG 1 cut(s) 937
Eco91I GGTNACC 1 cut(s) 1800
EcoICRI GAGCTC 1 cut(s) 1598
EcoNI CCTNNNNNAGG 1 cut(s) 654
EcoO109I RGGNCCY 3 cut(s) 647, 764, 1610
EcoO65I GGTNACC 1 cut(s) 1800
EcoRI GAATTC 1 cut(s) 946
EcoRII CCWGG 5 cut(s) 767, 1423, 1533, 1654, 1741
EcoRV GATATC 2 cut(s) 293, 2146
EcoT14I CCWWGG 4 cut(s) 131, 143, 744, 1276
EcoT22I ATGCAT 2 cut(s) 486, 1102
EcoT38I GRGCYC 2 cut(s) 768, 1600
ErhI CCWWGG 4 cut(s) 131, 143, 744, 1276
FaqI GGGAC 2 cut(s) 515, 1623
FauI CCCGC 4 cut(s) 460, 1011, 1062, 1807
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 9 cut(s) 84, 87, 189, 304, 1821, 2207, 2358, 2361, 2364
FokI GGATG 6 cut(s) 35, 185, 233, 407, 1034, 1640
FriOI GRGCYC 2 cut(s) 768, 1600
Fsp4HI GCNGC 9 cut(s) 84, 87, 189, 304, 1821, 2207, 2358, 2361, 2364
FspBI CTAG 3 cut(s) 215, 918, 1251
GlaI GCGC 1 cut(s) 1358
GluI GCNGC 9 cut(s) 84, 87, 189, 304, 1821, 2207, 2358, 2361, 2364
GsaI CCCAGC 2 cut(s) 1035, 1175
GsuI CTGGAG 1 cut(s) 1647
HaeIII GGCC 7 cut(s) 149, 306, 766, 1695, 1736, 2045, 2129
HapII CCGG 4 cut(s) 44, 782, 1298, 1601
HhaI GCGC 1 cut(s) 1359
Hin6I GCGC 1 cut(s) 1357
HinP1I GCGC 1 cut(s) 1357
HindIII AAGCTT 2 cut(s) 1145, 1440
HinfI GANTC 7 cut(s) 167, 554, 1127, 1160, 1433, 1484, 1511
HpaII CCGG 4 cut(s) 44, 782, 1298, 1601
HphI GGTGA 4 cut(s) 119, 130, 374, 1200
Hpy166II GTNNAC 2 cut(s) 157, 2232
Hpy188I TCNGA 7 cut(s) 163, 172, 868, 904, 1308, 1510, 2381
Hpy188III TCNNGA 5 cut(s) 44, 296, 831, 1488, 2278
Hpy8I GTNNAC 2 cut(s) 157, 2232
Hpy99I CGWCG 1 cut(s) 2377
HpyCH4III ACNGT 5 cut(s) 38, 494, 828, 1864, 2351
HpyCH4IV ACGT 1 cut(s) 852
HpyF10VI GCNNNNNNNGC 9 cut(s) 312, 613, 1394, 1538, 1820, 1826, 1835, 2126, 2363
HpyF3I CTNAG 8 cut(s) 192, 937, 1245, 1305, 1404, 1755, 1977, 2149
HpySE526I ACGT 1 cut(s) 852
HspAI GCGC 1 cut(s) 1357
KflI GGGWCCC 1 cut(s) 1610
Kpn2I TCCGGA 1 cut(s) 43
KpnI GGTACC 1 cut(s) 743
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
LmnI GCTCC 6 cut(s) 1246, 1402, 1595, 1603, 1826, 2071
Lsp1109I GCAGC 5 cut(s) 95, 1807, 2369, 2372, 2375
LweI GCATC 1 cut(s) 60
MaeI CTAG 3 cut(s) 215, 918, 1251
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 4 cut(s) 346, 1206, 1800, 1949
MalI GATC 8 cut(s) 42, 61, 141, 177, 684, 1378, 2099, 2196
MboI GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 8 cut(s) 582, 768, 1089, 1534, 1580, 1600, 1831, 2122
MlsI TGGCCA 1 cut(s) 2045
MluCI AATT 7 cut(s) 505, 862, 946, 1474, 1499, 1685, 1780
MluNI TGGCCA 1 cut(s) 2045
MlyI GAGTC 2 cut(s) 563, 1154
MmeI TCCRAC 1 cut(s) 2359
Mox20I TGGCCA 1 cut(s) 2045
Mph1103I ATGCAT 2 cut(s) 486, 1102
MroI TCCGGA 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 2055
MscI TGGCCA 1 cut(s) 2045
MseI TTAA 4 cut(s) 1155, 1632, 1647, 2427
MslI CAYNNNNRTG 3 cut(s) 162, 1202, 1344
Msp20I TGGCCA 1 cut(s) 2045
MspA1I CMGCKG 2 cut(s) 86, 467
MspI CCGG 4 cut(s) 44, 782, 1298, 1601
MspR9I CCNGG 5 cut(s) 769, 1425, 1535, 1656, 1743
MvaI CCWGG 5 cut(s) 769, 1425, 1535, 1656, 1743
MvnI CGCG 1 cut(s) 663
MwoI GCNNNNNNNGC 9 cut(s) 312, 613, 1394, 1538, 1820, 1826, 1835, 2126, 2363
NcoI CCATGG 1 cut(s) 1276
NdeII GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
NmeAIII GCCGAG 1 cut(s) 868
NmuCI GTSAC 1 cut(s) 1206
NsiI ATGCAT 2 cut(s) 486, 1102
NspI RCATGY 1 cut(s) 484
NspV TTCGAA 1 cut(s) 1482
PaqCI CACCTGC 1 cut(s) 2178
PcsI WCGNNNNNNNCGW 3 cut(s) 204, 523, 2274
PdmI GAANNNNTTC 1 cut(s) 2055
PfeI GAWTC 5 cut(s) 167, 1127, 1433, 1484, 1511
PflMI CCANNNNNTGG 2 cut(s) 1178, 1766
PfoI TCCNGGA 1 cut(s) 1741
PinAI ACCGGT 1 cut(s) 1297
PkrI GCNGC 9 cut(s) 85, 88, 190, 305, 1822, 2208, 2359, 2362, 2365
PleI GAGTC 2 cut(s) 562, 1154
PpsI GAGTC 2 cut(s) 562, 1154
PpuMI RGGWCCY 2 cut(s) 647, 1610
PshBI ATTAAT 1 cut(s) 1155
Psp124BI GAGCTC 1 cut(s) 1600
Psp5II RGGWCCY 2 cut(s) 647, 1610
Psp6I CCWGG 5 cut(s) 767, 1423, 1533, 1654, 1741
PspEI GGTNACC 1 cut(s) 1800
PspFI CCCAGC 2 cut(s) 1031, 1171
PspGI CCWGG 5 cut(s) 767, 1423, 1533, 1654, 1741
PspOMI GGGCCC 1 cut(s) 764
PspPI GGNCC 8 cut(s) 147, 387, 647, 764, 765, 1610, 1693, 2127
PspPPI RGGWCCY 2 cut(s) 647, 1610
PstI CTGCAG 1 cut(s) 1825
PstNI CAGNNNCTG 1 cut(s) 1178
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 9 cut(s) 197, 694, 741, 855, 1026, 1660, 2055, 2183, 2341
RsaNI GTAC 9 cut(s) 196, 693, 740, 854, 1025, 1659, 2054, 2182, 2340
RseI CAYNNNNRTG 3 cut(s) 162, 1202, 1344
SacI GAGCTC 1 cut(s) 1600
SaqAI TTAA 4 cut(s) 1155, 1632, 1647, 2427
SatI GCNGC 9 cut(s) 84, 87, 189, 304, 1821, 2207, 2358, 2361, 2364
Sau3AI GATC 8 cut(s) 40, 59, 139, 175, 682, 1376, 2097, 2194
Sau96I GGNCC 8 cut(s) 147, 387, 647, 764, 765, 1610, 1693, 2127
ScaI AGTACT 1 cut(s) 2055
SchI GAGTC 2 cut(s) 563, 1154
ScrFI CCNGG 5 cut(s) 769, 1425, 1535, 1656, 1743
SduI GDGCHC 8 cut(s) 582, 768, 1089, 1534, 1580, 1600, 1831, 2122
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 2 cut(s) 734, 1821
SfuI TTCGAA 1 cut(s) 1482
SinI GGWCC 3 cut(s) 387, 647, 1610
SmiMI CAYNNNNRTG 3 cut(s) 162, 1202, 1344
SmlI CTYRAG 3 cut(s) 1236, 1437, 1918
SmoI CTYRAG 3 cut(s) 1236, 1437, 1918
Sse9I AATT 7 cut(s) 505, 862, 946, 1474, 1499, 1685, 1780
SspMI CTAG 3 cut(s) 215, 918, 1251
SstI GAGCTC 1 cut(s) 1600
StyD4I CCNGG 5 cut(s) 767, 1423, 1533, 1654, 1741
StyI CCWWGG 4 cut(s) 131, 143, 744, 1276
TaaI ACNGT 5 cut(s) 38, 494, 828, 1864, 2351
TaiI ACGT 1 cut(s) 855
TaqI TCGA 4 cut(s) 417, 1375, 1482, 2268
TaqII GACCGA 2 cut(s) 1044, 2365
TasI AATT 7 cut(s) 505, 862, 946, 1474, 1499, 1685, 1780
TatI WGTACW 4 cut(s) 1024, 2053, 2181, 2339
TauI GCSGC 4 cut(s) 89, 191, 306, 2209
TfiI GAWTC 5 cut(s) 167, 1127, 1433, 1484, 1511
Tru1I TTAA 4 cut(s) 1155, 1632, 1647, 2427
Tru9I TTAA 4 cut(s) 1155, 1632, 1647, 2427
TscAI CASTG 6 cut(s) 43, 462, 1561, 1621, 1836, 2053
TseFI GTSAC 1 cut(s) 1206
TseI GCWGC 5 cut(s) 83, 1820, 2357, 2360, 2363
Tsp45I GTSAC 1 cut(s) 1206
TspGWI ACGGA 4 cut(s) 365, 379, 1575, 2361
TspRI CASTG 6 cut(s) 43, 462, 1561, 1621, 1836, 2053
Van91I CCANNNNNTGG 2 cut(s) 1178, 1766
VpaK11BI GGWCC 3 cut(s) 387, 647, 1610
VspI ATTAAT 1 cut(s) 1155
XagI CCTNNNNNAGG 1 cut(s) 654
XapI RAATTY 3 cut(s) 946, 1474, 1780
XceI RCATGY 1 cut(s) 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 1621
XmnI GAANNNNTTC 1 cut(s) 2055
XspI CTAG 3 cut(s) 215, 918, 1251
ZrmI AGTACT 1 cut(s) 2055
Zsp2I ATGCAT 2 cut(s) 486, 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.