Rmu_co8200514.1_g000001

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8200514.1
OG0005735
Physical Location & Seq
Forward (+)
124 .. 663
540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8200514.1_g000001.1.cds

Sequence Viewer

Length: 540 bp
atggctacggtgggggaggaagatgtggagtacgacagtgatccagaggaggttaaacgatcgctggcgatgaggaggcgggcggcggcgagcgacgatgaggaaggtgaaggagacgggagggagaaatcgaggccggatcggagggctcccattcactctgatgatgaatcggacgatcagggtggggcagcggagtatgatgacgacgaagaattggtagaagacgaagaagaagaagaagagattgatgaggaagaagaagagccgtttcatgaggccgcctatgtggagagagggcgtgaaggtgagactaggactgaggttttggcggtgaaggaatcggatggcggtgatgggacgaggtctgtggatggaacggtggatgagcatggagagaatcagaccggagaaggtgaggaggaggaggaggagaagaaggagaatgaaccctttgcagtgcccacagctggggcgttctatatgcatgatgaccgattcagggacaatgccggtggtcggaacaggtatgactcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Btz

Protein Analysis

179

Amino Acids

20.13

Weight (kDa)

4.09

Isoelectric Point (pI)

81.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 79, 83, 86, 194, 282, 332, 351
AclWI GGATC 2 cut(s) 35, 147
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 4 cut(s) 470, 471, 502, 519
AluBI AGCT 1 cut(s) 470
AluI AGCT 1 cut(s) 470
Alw26I GTCTC 2 cut(s) 108, 305
AlwI GGATC 2 cut(s) 35, 147
AoxI GGCC 2 cut(s) 134, 279
ApeKI GCWGC 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 310, 342
AsuHPI GGTGA 5 cut(s) 119, 320, 346, 365, 428
BaeGI GKGCMC 1 cut(s) 465
BanII GRGCYC 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 203
BccI CCATC 3 cut(s) 341, 350, 368
BceAI ACGGC 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 108, 305
BfaI CTAG 1 cut(s) 315
BisI GCNGC 4 cut(s) 84, 87, 192, 282
BlsI GCNGC 4 cut(s) 85, 88, 193, 283
BmiI GGNNCC 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 407
Bsc4I CCNNNNNNNGG 4 cut(s) 470, 471, 502, 519
Bse118I RCCGGY 1 cut(s) 512
BseGI GGATG 3 cut(s) 352, 379, 391
BseLI CCNNNNNNNGG 4 cut(s) 470, 471, 502, 519
BseMII CTCAG 1 cut(s) 312
BseRI GAGGAG 7 cut(s) 62, 88, 434, 437, 440, 443, 446
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 1 cut(s) 203
BseYI CCCAGC 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 136, 281
BsiEI CGRYCG 1 cut(s) 62
BsiSI CCGG 3 cut(s) 137, 408, 513
BslFI GGGAC 2 cut(s) 373, 518
BslI CCNNNNNNNGG 4 cut(s) 470, 471, 502, 519
BsmAI GTCTC 2 cut(s) 108, 305
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 2 cut(s) 373, 518
BsnI GGCC 2 cut(s) 136, 281
Bsp1286I GDGCHC 2 cut(s) 151, 465
Bsp143I GATC 4 cut(s) 40, 59, 139, 178
BspACI CCGC 7 cut(s) 79, 83, 86, 194, 282, 332, 351
BspANI GGCC 2 cut(s) 136, 281
BspCNI CTCAG 1 cut(s) 313
BspHI TCATGA 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 2 cut(s) 35, 147
BspQI GCTCTTC 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 512
BssAI RCCGGY 1 cut(s) 512
BssMI GATC 4 cut(s) 40, 59, 139, 178
Bst4CI ACNGT 3 cut(s) 10, 38, 382
Bst6I CTCTTC 2 cut(s) 237, 258
BstC8I GCNNGC 3 cut(s) 66, 81, 91
BstDEI CTNAG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 352, 379, 391
BstKTI GATC 4 cut(s) 43, 62, 142, 181
BstMAI GTCTC 2 cut(s) 108, 305
BstMBI GATC 4 cut(s) 40, 59, 139, 178
BstMCI CGRYCG 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 465
BstV1I GCAGC 1 cut(s) 203
BstV2I GAAGAC 1 cut(s) 231
BsuRI GGCC 2 cut(s) 136, 281
BtgZI GCGATG 1 cut(s) 83
BtsCI GGATG 3 cut(s) 352, 379, 391
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 43, 465
Cac8I GCNNGC 3 cut(s) 66, 81, 91
CciI TCATGA 1 cut(s) 274
Cfr10I RCCGGY 1 cut(s) 512
Csp6I GTAC 1 cut(s) 31
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviAII CATG 3 cut(s) 275, 392, 488
CviJI RGCY 6 cut(s) 5, 136, 149, 268, 281, 470
CviKI_1 RGCY 6 cut(s) 5, 136, 149, 268, 281, 470
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 321
DpnI GATC 4 cut(s) 42, 61, 141, 180
DpnII GATC 4 cut(s) 40, 59, 139, 178
Eam1104I CTCTTC 2 cut(s) 237, 258
EarI CTCTTC 2 cut(s) 237, 258
Eco24I GRGCYC 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 151
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 3 cut(s) 278, 395, 491
FaiI YATR 9 cut(s) 201, 276, 288, 393, 483, 485, 489, 531, 538
FaqI GGGAC 2 cut(s) 373, 518
FatI CATG 3 cut(s) 274, 391, 487
FauI CCCGC 1 cut(s) 72
Fnu4HI GCNGC 4 cut(s) 84, 87, 192, 282
FokI GGATG 3 cut(s) 359, 386, 398
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 4 cut(s) 84, 87, 192, 282
FspBI CTAG 1 cut(s) 315
GluI GCNGC 4 cut(s) 84, 87, 192, 282
GsaI CCCAGC 1 cut(s) 474
HaeIII GGCC 2 cut(s) 136, 281
HapII CCGG 3 cut(s) 137, 408, 513
Hin1II CATG 3 cut(s) 278, 395, 491
HinfI GANTC 5 cut(s) 170, 341, 400, 498, 533
HpaII CCGG 3 cut(s) 137, 408, 513
HphI GGTGA 5 cut(s) 119, 320, 346, 365, 428
Hpy188I TCNGA 6 cut(s) 144, 163, 175, 346, 405, 522
Hpy188III TCNNGA 2 cut(s) 44, 275
Hpy99I CGWCG 2 cut(s) 98, 212
HpyAV CCTTC 6 cut(s) 98, 104, 299, 331, 407, 433
HpyCH4III ACNGT 3 cut(s) 10, 38, 382
HpyCH4V TGCA 2 cut(s) 458, 487
HpyF3I CTNAG 1 cut(s) 321
Hsp92II CATG 3 cut(s) 278, 395, 491
Kzo9I GATC 4 cut(s) 40, 59, 139, 178
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 9 cut(s) 50, 57, 150, 167, 421, 456, 487, 511, 526
Lsp1109I GCAGC 1 cut(s) 203
MaeI CTAG 1 cut(s) 315
MalI GATC 4 cut(s) 42, 61, 141, 180
MboI GATC 4 cut(s) 40, 59, 139, 178
MhlI GDGCHC 2 cut(s) 151, 465
MluCI AATT 1 cut(s) 215
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 500
Mph1103I ATGCAT 1 cut(s) 489
MseI TTAA 1 cut(s) 54
MslI CAYNNNNRTG 1 cut(s) 162
MspA1I CMGCKG 2 cut(s) 194, 470
MspI CCGG 3 cut(s) 137, 408, 513
NdeII GATC 4 cut(s) 40, 59, 139, 178
NlaIII CATG 3 cut(s) 278, 395, 491
NlaIV GGNNCC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 489
PagI TCATGA 1 cut(s) 274
PciSI GCTCTTC 1 cut(s) 258
PfeI GAWTC 4 cut(s) 170, 341, 400, 498
PflFI GACNNNGTC 1 cut(s) 364
PkrI GCNGC 4 cut(s) 85, 88, 193, 283
Ple19I CGATCG 1 cut(s) 62
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
PspFI CCCAGC 1 cut(s) 470
PspN4I GGNNCC 1 cut(s) 150
PsyI GACNNNGTC 1 cut(s) 364
PvuI CGATCG 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 4 cut(s) 84, 87, 192, 282
Sau3AI GATC 4 cut(s) 40, 59, 139, 178
SchI GAGTC 1 cut(s) 527
SduI GDGCHC 2 cut(s) 151, 465
SetI ASST 8 cut(s) 54, 109, 310, 327, 368, 418, 472, 530
SmiMI CAYNNNNRTG 1 cut(s) 162
Sse9I AATT 1 cut(s) 215
SsiI CCGC 7 cut(s) 79, 83, 86, 194, 282, 332, 351
SspMI CTAG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 10, 38, 382
TaqI TCGA 1 cut(s) 131
TaqII GACCGA 1 cut(s) 510
TasI AATT 1 cut(s) 215
TauI GCSGC 3 cut(s) 86, 89, 284
TfiI GAWTC 4 cut(s) 170, 341, 400, 498
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 2 cut(s) 43, 465
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 3 cut(s) 183, 263, 462
TspRI CASTG 2 cut(s) 43, 465
Tth111I GACNNNGTC 1 cut(s) 364
XspI CTAG 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.