Rw2G032040

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005735
Physical Location & Seq
Reverse (-)
52813828 .. 52817881
4054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032040.1

Sequence Viewer

Length: 2055 bp
ATGGCTACGGTGGGGGAGGAAGATGTGGAGTACGACAGTGATCCAGAGGAGGTTAAACGATCGCTGGCGATGAGGAGGCGGGCGGCGGCGAGCGACGATGAGGAAGGTGAAGGAGAGGGGAGGGAGAAATCGAGGCCGGATCGGAGGGCTCCCATTCACTCTGATGATGAATCGGACGATCAGGGTGGGGCAGCGGAGTATGACGACGACGAAGAATTGGTAGAAGACGAAGAAGAAGAAGAAGAGATTGATGAGGAAGAAGAAGAGCCGTTTCATGAGGCCGCCTATGTGGAGAGAGGGCGTGAAGGTGAGACTAGGACTGAGGTTTTGGCGGTGAAGGAATCGGATGGCGGTGATGGGACGAGGTCTGTGGATGGAACGGTGGATGAGCATGGAGAGAATCAGACCCGAGAAGGTGAGGAGGAGGAGGAGGAGAAGAAGGAGAATGAACCCTTTGCAGTGCCCACAGCTGGGGCGTTCTATATGCATGATGACCGATTCAGGGACAATGCCGGTGGTCGGAACAGGCGAACACATGGTGGAAGGAAGCTATGGGAGTCAAAAGATGACAGGAAGTGGGGGCATGACAAGTTTGAGGAGATAACTTTGCACGAAAGGCACCACGAAGAGGGCAGGAGACCTTCTAGGGGAAATTTTCGGGGTAGAGGAAGAAACCGAGGTATTGATCGTGGGTACGCTACAGGAAACAGGTCTAAAGAGTATGAGAATAATAATCAGAGACCGGTACCCAAGGGTGTGAGAGGGAGAGGGCCCAGAAGATATGAACCTGCTTTTAGGAATAACAGACAGACGCCTTCCACACAAAATAGACAGTCTGGAAAGCCTGTTGACAAAACTTCACAAACTAATTCAGAGAGGACTTTTGCTCCCCCATCGAATGCAGAATCTGACTCAGTTCCTGCTAGGAAAAATGTCTTTGCATCAAGCCTGAATTCAGCTTCTCCTCCTTTTTATCCATCAGGATCCTCCAATAAGGAGATCAATCTTAATCAAAAAAGGGATGCACATGCTGGGAGTGCTAAGAGGAATGCTCGTCCATCTGTATCAGTGGAGAATTTTTCTGGGCGACAAACCAATGCATATCTGCGAGGGAAGAATGTAGCTGAATCTGTTGGCGTTGGAATGGAAAAGCTTTATATTGATGATTCGAAGAATCCAGGAGCTGGGAAGACATTGACCACTGTACAAATGCCATCATCTGGGTCTTCAATTGTCAACGCTACTCAACCTTCTCAGTCTAGGGTTCAGGGAAGGGGTGCTGTCACAGGACAGATGAGTTATCGACCTGTCGTTTCTCAGAACCAGATGCCAAGAGCTCCATCACAACAGTTCCATGCTGTTCAGCGTAATCCCGTCCAAAATCGGTCCCAACTAGCTCTTCAAGCTCCTCCCCAGCAGGTGGGACAACGCCCTGGAAGTGGATCTCAATCCTCCTCTCCACCTAAAACACCTGGAGAAGTTGAACCTGCAGATTCAAGTAAATCTAAAGGTGCACTAGTTGCAAAGGGAAAAGGCAGTGCTCTAGGTAATGGAAGGGGAGCACTTACTTACAATGGGGCTCAGGTTATTGGGGCTTCTGGAGGCATGGGTGTCAATCATGGAGATCAGAACTTTCCTGGGACTCCTGCCTTTTTGCCTGTTATGCAATTTGGTGGCCAGCACCCTGGGGGTATGGGAGTTCCTGCTGTTGGCATGGCATTCCCTGGATATGTTGCTCAGCCACAACTTGGTGGTGGTATGGGAAATTCTGAAATGACCTGGTTACCTGTATTAGCGGGTGCTGCAGGAGCTTTAGGGGCTGCCTATTGTCCACCCTATATTACTGTTGATGGTTCTTATCATGCTCGCCCGTCAGGACAGACATCTGTGGGTTCCTTGAGCAAAGAAAATAATGCAAACAAAACCAATAATGAATGGAAGCCTTCACAAAAGCCTGTGGGCGATGATTACGGGCAGCGACAAAACAAGCCTCGTAGATATTCTCAGATGAATGTCGCCAGTGAAGTTCTTCAAAATAATACGGAGCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Btz

Protein Analysis

684

Amino Acids

74.32

Weight (kDa)

5.72

Isoelectric Point (pI)

57.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Btz PF09405 119 - 224 6.2e-20 CASC3/Barentsz eIF4AIII binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1408
Acc36I ACCTGC 3 cut(s) 796, 1408, 1495
Acc65I GGTACC 1 cut(s) 745
AccB1I GGYRCC 2 cut(s) 618, 745
AccB7I CCANNNNNTGG 4 cut(s) 1184, 1220, 1420, 1748
AciI CCGC 8 cut(s) 79, 83, 86, 194, 282, 332, 351, 1796
AclWI GGATC 5 cut(s) 35, 147, 978, 991, 1450
AcoI YGGCCR 1 cut(s) 1675
AcsI RAATTY 4 cut(s) 652, 952, 1075, 1765
AcyI GRCGYC 1 cut(s) 812
AdeI CACNNNGTG 1 cut(s) 539
AfaI GTAC 4 cut(s) 32, 695, 747, 1206
AgeI ACCGGT 1 cut(s) 742
AgsI TTSAA 5 cut(s) 1230, 1403, 1484, 1497, 2033
AhlI ACTAGT 1 cut(s) 1516
AjnI CCWGG 7 cut(s) 1177, 1432, 1471, 1636, 1684, 1723, 1778
AjuI GAANNNNNNNTTGG 2 cut(s) 1383, 1415
Alw21I GWGCWC 4 cut(s) 1339, 1516, 1543, 1564
Alw26I GTCTC 3 cut(s) 305, 631, 733
Alw44I GTGCAC 1 cut(s) 1512
AlwI GGATC 5 cut(s) 35, 147, 978, 991, 1450
AlwNI CAGNNNCTG 3 cut(s) 908, 920, 1184
Ama87I CYCGRG 1 cut(s) 408
AoxI GGCC 4 cut(s) 134, 279, 770, 1675
ApaI GGGCCC 1 cut(s) 774
ApaLI GTGCAC 1 cut(s) 1512
ApeKI GCWGC 4 cut(s) 191, 1802, 1820, 1975
ApoI RAATTY 4 cut(s) 652, 952, 1075, 1765
ArsI GACNNNNNNTTYG 2 cut(s) 310, 342
AsiGI ACCGGT 1 cut(s) 742
Asp700I GAANNNNTTC 1 cut(s) 2028
Asp718I GGTACC 1 cut(s) 745
AspS9I GGNCC 3 cut(s) 770, 771, 1386
AsuHPI GGTGA 5 cut(s) 119, 320, 346, 365, 428
AsuII TTCGAA 1 cut(s) 1169
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 1386
BaeGI GKGCMC 3 cut(s) 465, 774, 1516
BalI TGGCCA 1 cut(s) 1677
BamHI GGATCC 1 cut(s) 983
BanI GGYRCC 2 cut(s) 618, 745
BanII GRGCYC 4 cut(s) 151, 774, 1339, 1582
BbsI GAAGAC 3 cut(s) 231, 1196, 1218
Bbv12I GWGCWC 4 cut(s) 1339, 1516, 1543, 1564
BbvI GCAGC 4 cut(s) 203, 1789, 1807, 1987
BccI CCATC 9 cut(s) 341, 350, 368, 901, 985, 1066, 1222, 1348, 1844
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
BcoDI GTCTC 3 cut(s) 305, 631, 733
BcuI ACTAGT 1 cut(s) 1516
BfaI CTAG 7 cut(s) 315, 645, 924, 1260, 1394, 1517, 1544
BfmI CTRYAG 3 cut(s) 699, 1488, 1803
BfuAI ACCTGC 3 cut(s) 796, 1408, 1495
BisI GCNGC 7 cut(s) 84, 87, 192, 282, 1803, 1821, 1976
BlpI GCTNAGC 1 cut(s) 1737
BlsI GCNGC 7 cut(s) 85, 88, 193, 283, 1804, 1822, 1977
Bme1390I CCNGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
Bme18I GGWCC 1 cut(s) 1386
BmeT110I CYCGRG 1 cut(s) 408
BmgT120I GGNCC 3 cut(s) 770, 771, 1386
BmiI GGNNCC 7 cut(s) 150, 620, 747, 772, 985, 1388, 1894
BmrFI CCNGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
BmsI GCATC 3 cut(s) 950, 1012, 1317
BpiI GAAGAC 3 cut(s) 231, 1196, 1218
BplI GAGNNNNNCTC 2 cut(s) 1036, 1068
BpmI CTGGAG 2 cut(s) 1494, 1620
Bpu10I CCTNAGC 1 cut(s) 1581
Bpu1102I GCTNAGC 1 cut(s) 1737
Bpu14I TTCGAA 1 cut(s) 1169
BpuEI CTTGAG 1 cut(s) 1918
BsaBI GATNNNNATC 1 cut(s) 1447
BsaHI GRCGYC 1 cut(s) 812
BsaI GGTCTC 2 cut(s) 631, 733
BsaJI CCNNGG 7 cut(s) 676, 750, 1432, 1637, 1684, 1685, 1723
BsaWI WCCGGW 1 cut(s) 742
BsaXI ACNNNNNCTCC 2 cut(s) 871, 901
Bse118I RCCGGY 2 cut(s) 512, 742
Bse1I ACTGG 1 cut(s) 2019
Bse8I GATNNNNATC 1 cut(s) 1447
BseBI CCWGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
BseDI CCNNGG 7 cut(s) 676, 750, 1432, 1637, 1684, 1685, 1723
BseGI GGATG 4 cut(s) 352, 379, 391, 1027
BseJI GATNNNNATC 1 cut(s) 1447
BseMII CTCAG 7 cut(s) 312, 927, 1268, 1331, 1595, 1751, 2018
BseNI ACTGG 1 cut(s) 2019
BseSI GKGCMC 3 cut(s) 465, 774, 1516
BseXI GCAGC 4 cut(s) 203, 1789, 1807, 1987
BseYI CCCAGC 4 cut(s) 470, 1031, 1184, 1413
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 4 cut(s) 136, 281, 772, 1677
BshNI GGYRCC 2 cut(s) 618, 745
BshTI ACCGGT 1 cut(s) 742
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 4 cut(s) 1339, 1516, 1543, 1564
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 3 cut(s) 137, 513, 743
BslFI GGGAC 5 cut(s) 373, 518, 1372, 1437, 1654
BsmAI GTCTC 3 cut(s) 305, 631, 733
BsmFI GGGAC 5 cut(s) 373, 518, 1372, 1437, 1654
BsmI GAATGC 3 cut(s) 904, 1054, 1718
BsnI GGCC 4 cut(s) 136, 281, 772, 1677
Bso31I GGTCTC 2 cut(s) 631, 733
BsoBI CYCGRG 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 1169
Bsp120I GGGCCC 1 cut(s) 770
Bsp1286I GDGCHC 8 cut(s) 151, 465, 774, 1339, 1516, 1543, 1564, 1582
Bsp1407I TGTACA 1 cut(s) 1204
Bsp143I GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
Bsp1720I GCTNAGC 1 cut(s) 1737
BspACI CCGC 8 cut(s) 79, 83, 86, 194, 282, 332, 351, 1796
BspANI GGCC 4 cut(s) 136, 281, 772, 1677
BspCNI CTCAG 7 cut(s) 313, 926, 1267, 1330, 1594, 1750, 2017
BspHI TCATGA 1 cut(s) 274
BspLI GGNNCC 7 cut(s) 150, 620, 747, 772, 985, 1388, 1894
BspMAI CTGCAG 2 cut(s) 1492, 1807
BspMI ACCTGC 3 cut(s) 796, 1408, 1495
BspPI GGATC 5 cut(s) 35, 147, 978, 991, 1450
BspQI GCTCTTC 2 cut(s) 258, 1404
BspT104I TTCGAA 1 cut(s) 1169
BspT107I GGYRCC 2 cut(s) 618, 745
BspTNI GGTCTC 2 cut(s) 631, 733
BsrFI RCCGGY 2 cut(s) 512, 742
BsrGI TGTACA 1 cut(s) 1204
BsrI ACTGG 1 cut(s) 2019
BssAI RCCGGY 2 cut(s) 512, 742
BssECI CCNNGG 7 cut(s) 676, 750, 1432, 1637, 1684, 1685, 1723
BssMI GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
BssNI GRCGYC 1 cut(s) 812
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
Bst4CI ACNGT 7 cut(s) 10, 38, 382, 834, 1204, 1350, 1846
Bst6I CTCTTC 4 cut(s) 237, 258, 621, 1404
BstACI GRCGYC 1 cut(s) 812
BstAPI GCANNNNNTGC 1 cut(s) 1520
BstAUI TGTACA 1 cut(s) 1204
BstBI TTCGAA 1 cut(s) 1169
BstC8I GCNNGC 5 cut(s) 66, 81, 91, 1679, 1867
BstDEI CTNAG 8 cut(s) 321, 913, 1041, 1254, 1317, 1581, 1737, 2004
BstEII GGTNACC 1 cut(s) 1782
BstF5I GGATG 4 cut(s) 352, 379, 391, 1027
BstKTI GATC 9 cut(s) 43, 62, 142, 181, 688, 986, 1002, 1445, 1627
BstMAI GTCTC 3 cut(s) 305, 631, 733
BstMBI GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 8 cut(s) 616, 1037, 1403, 1520, 1663, 1802, 1808, 1817
BstNI CCWGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
BstNSI RCATGY 1 cut(s) 1031
BstPI GGTNACC 1 cut(s) 1782
BstSCI CCNGG 7 cut(s) 1177, 1432, 1471, 1636, 1684, 1723, 1778
BstSFI CTRYAG 3 cut(s) 699, 1488, 1803
BstSLI GKGCMC 3 cut(s) 465, 774, 1516
BstV1I GCAGC 4 cut(s) 203, 1789, 1807, 1987
BstV2I GAAGAC 3 cut(s) 231, 1196, 1218
BstX2I RGATCY 2 cut(s) 983, 1442
BstXI CCANNNNNNTGG 1 cut(s) 1685
BstYI RGATCY 2 cut(s) 983, 1442
BsuRI GGCC 4 cut(s) 136, 281, 772, 1677
BtgZI GCGATG 2 cut(s) 83, 1977
BtsCI GGATG 4 cut(s) 352, 379, 391, 1027
BtsI GCAGTG 2 cut(s) 465, 1543
BtsIMutI CAGTG 6 cut(s) 43, 465, 1074, 1200, 1543, 2026
BveI ACCTGC 3 cut(s) 796, 1408, 1495
Cac8I GCNNGC 5 cut(s) 66, 81, 91, 1679, 1867
CaiI CAGNNNCTG 3 cut(s) 908, 920, 1184
CciI TCATGA 1 cut(s) 274
Cfr10I RCCGGY 2 cut(s) 512, 742
Cfr13I GGNCC 3 cut(s) 770, 771, 1386
CseI GACGC 1 cut(s) 820
CsiI ACCWGGT 1 cut(s) 1778
Csp6I GTAC 4 cut(s) 31, 694, 746, 1205
CspAI ACCGGT 1 cut(s) 742
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviQI GTAC 4 cut(s) 31, 694, 746, 1205
DdeI CTNAG 8 cut(s) 321, 913, 1041, 1254, 1317, 1581, 1737, 2004
DpnI GATC 9 cut(s) 42, 61, 141, 180, 687, 985, 1001, 1444, 1626
DpnII GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
DraIII CACNNNGTG 1 cut(s) 539
EaeI YGGCCR 1 cut(s) 1675
Eam1104I CTCTTC 4 cut(s) 237, 258, 621, 1404
EarI CTCTTC 4 cut(s) 237, 258, 621, 1404
Ecl136II GAGCTC 1 cut(s) 1337
Eco130I CCWWGG 1 cut(s) 750
Eco24I GRGCYC 4 cut(s) 151, 774, 1339, 1582
Eco31I GGTCTC 2 cut(s) 631, 733
Eco47I GGWCC 1 cut(s) 1386
Eco53kI GAGCTC 1 cut(s) 1337
Eco88I CYCGRG 1 cut(s) 408
Eco91I GGTNACC 1 cut(s) 1782
EcoICRI GAGCTC 1 cut(s) 1337
EcoO109I RGGNCCY 1 cut(s) 770
EcoO65I GGTNACC 1 cut(s) 1782
EcoRI GAATTC 1 cut(s) 952
EcoRII CCWGG 7 cut(s) 1177, 1432, 1471, 1636, 1684, 1723, 1778
EcoT14I CCWWGG 1 cut(s) 750
EcoT22I ATGCAT 2 cut(s) 489, 1102
EcoT38I GRGCYC 4 cut(s) 151, 774, 1339, 1582
ErhI CCWWGG 1 cut(s) 750
FaqI GGGAC 5 cut(s) 373, 518, 1372, 1437, 1654
FauI CCCGC 2 cut(s) 72, 1789
Fnu4HI GCNGC 7 cut(s) 84, 87, 192, 282, 1803, 1821, 1976
FokI GGATG 4 cut(s) 359, 386, 398, 1034
FriOI GRGCYC 4 cut(s) 151, 774, 1339, 1582
Fsp4HI GCNGC 7 cut(s) 84, 87, 192, 282, 1803, 1821, 1976
FspBI CTAG 7 cut(s) 315, 645, 924, 1260, 1394, 1517, 1544
GluI GCNGC 7 cut(s) 84, 87, 192, 282, 1803, 1821, 1976
GsaI CCCAGC 4 cut(s) 474, 1035, 1188, 1417
GsuI CTGGAG 2 cut(s) 1494, 1620
HaeIII GGCC 4 cut(s) 136, 281, 772, 1677
HapII CCGG 3 cut(s) 137, 513, 743
HgaI GACGC 1 cut(s) 820
Hin1I GRCGYC 1 cut(s) 812
HincII GTYRAC 2 cut(s) 850, 1237
HindII GTYRAC 2 cut(s) 850, 1237
HindIII AAGCTT 1 cut(s) 1151
HpaII CCGG 3 cut(s) 137, 513, 743
HphI GGTGA 5 cut(s) 119, 320, 346, 365, 428
Hpy166II GTNNAC 4 cut(s) 850, 1237, 1514, 1832
Hpy188III TCNNGA 6 cut(s) 44, 275, 837, 981, 1599, 1875
Hpy8I GTNNAC 4 cut(s) 850, 1237, 1514, 1832
Hpy99I CGWCG 3 cut(s) 98, 209, 212
HpyCH4III ACNGT 7 cut(s) 10, 38, 382, 834, 1204, 1350, 1846
HpyF10VI GCNNNNNNNGC 8 cut(s) 616, 1037, 1403, 1520, 1663, 1802, 1808, 1817
HpyF3I CTNAG 8 cut(s) 321, 913, 1041, 1254, 1317, 1581, 1737, 2004
Hsp92I GRCGYC 1 cut(s) 812
KpnI GGTACC 1 cut(s) 749
Kzo9I GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
LguI GCTCTTC 2 cut(s) 258, 1404
LmnI GCTCC 8 cut(s) 154, 892, 1181, 1342, 1411, 1559, 1808, 2044
Lsp1109I GCAGC 4 cut(s) 203, 1789, 1807, 1987
LweI GCATC 3 cut(s) 950, 1012, 1317
MabI ACCWGGT 1 cut(s) 1778
MaeI CTAG 7 cut(s) 315, 645, 924, 1260, 1394, 1517, 1544
MaeIII GTNAC 2 cut(s) 1282, 1782
MalI GATC 9 cut(s) 42, 61, 141, 180, 687, 985, 1001, 1444, 1626
MboI GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
MfeI CAATTG 1 cut(s) 1230
MflI RGATCY 2 cut(s) 983, 1442
MhlI GDGCHC 8 cut(s) 151, 465, 774, 1339, 1516, 1543, 1564, 1582
MlsI TGGCCA 1 cut(s) 1677
MluCI AATT 8 cut(s) 215, 652, 868, 952, 1075, 1230, 1667, 1765
MluNI TGGCCA 1 cut(s) 1677
MlyI GAGTC 3 cut(s) 566, 905, 1636
MmeI TCCRAC 2 cut(s) 500, 1120
Mox20I TGGCCA 1 cut(s) 1677
Mph1103I ATGCAT 2 cut(s) 489, 1102
MroXI GAANNNNTTC 1 cut(s) 2028
MscI TGGCCA 1 cut(s) 1677
MseI TTAA 2 cut(s) 54, 1008
MslI CAYNNNNRTG 1 cut(s) 162
Msp20I TGGCCA 1 cut(s) 1677
MspA1I CMGCKG 2 cut(s) 194, 470
MspI CCGG 3 cut(s) 137, 513, 743
MspR9I CCNGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
MunI CAATTG 1 cut(s) 1230
Mva1269I GAATGC 3 cut(s) 904, 1054, 1718
MvaI CCWGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
MwoI GCNNNNNNNGC 8 cut(s) 616, 1037, 1403, 1520, 1663, 1802, 1808, 1817
NdeII GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
NlaIV GGNNCC 7 cut(s) 150, 620, 747, 772, 985, 1388, 1894
NmuCI GTSAC 1 cut(s) 1282
NsiI ATGCAT 2 cut(s) 489, 1102
NspI RCATGY 1 cut(s) 1031
NspV TTCGAA 1 cut(s) 1169
PagI TCATGA 1 cut(s) 274
PaqCI CACCTGC 1 cut(s) 1408
PasI CCCWGGG 1 cut(s) 1685
PciSI GCTCTTC 2 cut(s) 258, 1404
PcsI WCGNNNNNNNCGW 1 cut(s) 526
PctI GAATGC 3 cut(s) 904, 1054, 1718
PdmI GAANNNNTTC 1 cut(s) 2028
PfeI GAWTC 9 cut(s) 170, 341, 400, 498, 905, 1127, 1166, 1174, 1493
PflFI GACNNNGTC 1 cut(s) 364
PflMI CCANNNNNTGG 4 cut(s) 1184, 1220, 1420, 1748
PfoI TCCNGGA 1 cut(s) 1177
PinAI ACCGGT 1 cut(s) 742
PkrI GCNGC 7 cut(s) 85, 88, 193, 283, 1804, 1822, 1977
Ple19I CGATCG 1 cut(s) 62
PleI GAGTC 3 cut(s) 565, 905, 1636
PpsI GAGTC 3 cut(s) 565, 905, 1636
Psp124BI GAGCTC 1 cut(s) 1339
Psp6I CCWGG 7 cut(s) 1177, 1432, 1471, 1636, 1684, 1723, 1778
PspEI GGTNACC 1 cut(s) 1782
PspFI CCCAGC 4 cut(s) 470, 1031, 1184, 1413
PspGI CCWGG 7 cut(s) 1177, 1432, 1471, 1636, 1684, 1723, 1778
PspN4I GGNNCC 7 cut(s) 150, 620, 747, 772, 985, 1388, 1894
PspOMI GGGCCC 1 cut(s) 770
PspPI GGNCC 3 cut(s) 770, 771, 1386
PstI CTGCAG 2 cut(s) 1492, 1807
PstNI CAGNNNCTG 3 cut(s) 908, 920, 1184
PsuI RGATCY 2 cut(s) 983, 1442
PsyI GACNNNGTC 1 cut(s) 364
PvuI CGATCG 1 cut(s) 62
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 4 cut(s) 32, 695, 747, 1206
RsaNI GTAC 4 cut(s) 31, 694, 746, 1205
RseI CAYNNNNRTG 1 cut(s) 162
SacI GAGCTC 1 cut(s) 1339
SapI GCTCTTC 2 cut(s) 258, 1404
SaqAI TTAA 2 cut(s) 54, 1008
SatI GCNGC 7 cut(s) 84, 87, 192, 282, 1803, 1821, 1976
Sau3AI GATC 9 cut(s) 40, 59, 139, 178, 685, 983, 999, 1442, 1624
Sau96I GGNCC 3 cut(s) 770, 771, 1386
SchI GAGTC 3 cut(s) 566, 905, 1636
ScrFI CCNGG 7 cut(s) 1179, 1434, 1473, 1638, 1686, 1725, 1780
SduI GDGCHC 8 cut(s) 151, 465, 774, 1339, 1516, 1543, 1564, 1582
SexAI ACCWGGT 1 cut(s) 1778
SfaNI GCATC 3 cut(s) 950, 1012, 1317
SfcI CTRYAG 3 cut(s) 699, 1488, 1803
SfuI TTCGAA 1 cut(s) 1169
SinI GGWCC 1 cut(s) 1386
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 1897
SmoI CTYRAG 1 cut(s) 1897
SpeI ACTAGT 1 cut(s) 1516
Sse9I AATT 8 cut(s) 215, 652, 868, 952, 1075, 1230, 1667, 1765
SsiI CCGC 8 cut(s) 79, 83, 86, 194, 282, 332, 351, 1796
SspMI CTAG 7 cut(s) 315, 645, 924, 1260, 1394, 1517, 1544
SstI GAGCTC 1 cut(s) 1339
StyD4I CCNGG 7 cut(s) 1177, 1432, 1471, 1636, 1684, 1723, 1778
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 7 cut(s) 10, 38, 382, 834, 1204, 1350, 1846
TaqI TCGA 4 cut(s) 131, 896, 1169, 1303
TaqII GACCGA 2 cut(s) 510, 1374
TasI AATT 8 cut(s) 215, 652, 868, 952, 1075, 1230, 1667, 1765
TatI WGTACW 1 cut(s) 1204
TauI GCSGC 3 cut(s) 86, 89, 284
TfiI GAWTC 9 cut(s) 170, 341, 400, 498, 905, 1127, 1166, 1174, 1493
Tru1I TTAA 2 cut(s) 54, 1008
Tru9I TTAA 2 cut(s) 54, 1008
TscAI CASTG 6 cut(s) 43, 465, 1074, 1207, 1543, 2026
TseFI GTSAC 1 cut(s) 1282
TseI GCWGC 4 cut(s) 191, 1802, 1820, 1975
Tsp45I GTSAC 1 cut(s) 1282
TspDTI ATGAA 6 cut(s) 183, 263, 462, 798, 1947, 2024
TspRI CASTG 6 cut(s) 43, 465, 1074, 1207, 1543, 2026
Tth111I GACNNNGTC 1 cut(s) 364
Van91I CCANNNNNTGG 4 cut(s) 1184, 1220, 1420, 1748
VneI GTGCAC 1 cut(s) 1512
VpaK11BI GGWCC 1 cut(s) 1386
XapI RAATTY 4 cut(s) 652, 952, 1075, 1765
XceI RCATGY 1 cut(s) 1031
XmnI GAANNNNTTC 1 cut(s) 2028
XspI CTAG 7 cut(s) 315, 645, 924, 1260, 1394, 1517, 1544
Zsp2I ATGCAT 2 cut(s) 489, 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.