MD17G1197300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005735
Physical Location & Seq
Forward (+)
23638768 .. 23640652
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1197300.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGGCTTCTATGGGTAAGGAGGATGTGGAGTATGAGAGTGATCCGGAGGTAACGAAGCGATCTCTGGCGATGCGGAGAAGGGCAGCGGCCAGCGACGATGAGGAAGGTGAAGAGGAGGCGACGGAGAAGCCGAGGAAGGATCCGAGAGCCGCGGTTCACTCCGATGAATCGGATGATCAGGGTGGGGCGGCTGAGTATGATGATGACGAAGAACTAGAAGAAGAAGAAGGAGAAGAAGAGGAAGAGATTGGTGAAGAAGAAGAGTTTGAGGAGGAAGTTGTGGAAGAAGAGGAGTTGGAAAGATATCAGGAGAGAGGCCGGGAAGGCGAGCTGGGTGGCAGGGTTGAGGTTTTGGCTGTTAAGGAATCGAGTGGTGATGGTCGGAGGTCTGTGGAAGGGTCCGTGGATGAGAATGGGGGGAATGAGGCTGAAGAAGGGGAGGAGAAGAAGGAGAATGAACCATTTGCAGTCCCCACAGCGGGTGCTTTTTATATGCATGATGACCGCTTCAGGGAAAACTCTGGTGGTCGGCACAGGCGAACACGTGGTGGAAGGAAGCTGTGGGAATCCAAAGATGAAAGAAAATGGGGGCACGATAAGTTTGAGGAGATTACTTTGCAGGAAAGGCGCTACGAAGAAGGAAAGAAACCTTCAAAGGGTCCTTATGTAGGTCGCGGAAGAAACCGAGGTATTGATCGTGGGAGTGCCAGAGGAAACAGGTCTAAAGAATATGACAGGTCTAAAGAATATGACAATAATAATCAGAGACAGGTACCCAAGGGTGTTAGAGGGAGAGGGCCCAGGAGGTATGAACCGGCTTTTAGGAATAACAGACAGGCACCGCCAACACAAAAAACAAACAGTGAGTACAACGTATTCTTATGGTCTGGAAAGCCTGCCGAGAAAAACGTACAAAGTAATTTAGAGAGAACATATACTCCCAACTCAAATGCAGAGTCTGGCCAGGTTTCTGCTAGGAAAAAAATTCCTTCGCCTCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Btz

Protein Analysis

335

Amino Acids

38.14

Weight (kDa)

4.87

Isoelectric Point (pI)

66.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Btz PF09405 136 - 220 1.4e-19 CASC3/Barentsz eIF4AIII binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 772
AccB1I GGYRCC 2 cut(s) 772, 838
AccII CGCG 2 cut(s) 152, 675
AccIII TCCGGA 1 cut(s) 43
AciI CCGC 9 cut(s) 73, 86, 150, 152, 188, 479, 505, 675, 842
AclWI GGATC 3 cut(s) 35, 134, 147
AcoI YGGCCR 2 cut(s) 87, 961
AcsI RAATTY 1 cut(s) 984
AcuI CTGAAG 2 cut(s) 450, 493
AcvI CACGTG 1 cut(s) 545
AdeI CACNNNGTG 1 cut(s) 548
AfaI GTAC 3 cut(s) 774, 869, 912
AfiI CCNNNNNNNGG 5 cut(s) 478, 479, 511, 656, 668
AflIII ACRYGT 1 cut(s) 542
AgsI TTSAA 1 cut(s) 654
AjnI CCWGG 2 cut(s) 800, 963
AloI GAACNNNNNNTCC 2 cut(s) 922, 954
AluBI AGCT 3 cut(s) 331, 559, 1001
AluI AGCT 3 cut(s) 331, 559, 1001
Alw26I GTCTC 1 cut(s) 760
AlwI GGATC 3 cut(s) 35, 134, 147
AlwNI CAGNNNCTG 1 cut(s) 959
Aor13HI TCCGGA 1 cut(s) 43
AoxI GGCC 4 cut(s) 87, 316, 797, 961
ApaI GGGCCC 1 cut(s) 801
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 984
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
Asp718I GGTACC 1 cut(s) 772
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 4 cut(s) 399, 659, 797, 798
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 3 cut(s) 119, 263, 386
AvaII GGWCC 2 cut(s) 399, 659
BaeGI GKGCMC 2 cut(s) 594, 801
BalI TGGCCA 1 cut(s) 963
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 2 cut(s) 772, 838
BanII GRGCYC 1 cut(s) 801
BbrPI CACGTG 1 cut(s) 545
BbvI GCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 371
BciT130I CCWGG 2 cut(s) 802, 965
BclI TGATCA 1 cut(s) 175
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 1 cut(s) 760
BfaI CTAG 2 cut(s) 215, 975
BfoI RGCGCY 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 324
BisI GCNGC 4 cut(s) 84, 87, 150, 189
BlsI GCNGC 4 cut(s) 85, 88, 151, 190
Bme1390I CCNGG 3 cut(s) 320, 802, 965
Bme18I GGWCC 2 cut(s) 399, 659
BmgT120I GGNCC 4 cut(s) 399, 659, 797, 798
BmiI GGNNCC 6 cut(s) 141, 400, 660, 774, 799, 840
BmrFI CCNGG 3 cut(s) 320, 802, 965
BmsI GCATC 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 981
BpuMI CCSGG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 545
BsaJI CCNNGG 6 cut(s) 131, 150, 402, 685, 777, 800
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 922, 952
Bsc4I CCNNNNNNNGG 5 cut(s) 478, 479, 511, 656, 668
Bse118I RCCGGY 1 cut(s) 814
BseAI TCCGGA 1 cut(s) 43
BseBI CCWGG 2 cut(s) 802, 965
BseDI CCNNGG 6 cut(s) 131, 150, 402, 685, 777, 800
BseGI GGATG 3 cut(s) 28, 178, 412
BseLI CCNNNNNNNGG 5 cut(s) 478, 479, 511, 656, 668
BseMII CTCAG 1 cut(s) 183
BseRI GAGGAG 5 cut(s) 128, 284, 305, 455, 620
BseSI GKGCMC 2 cut(s) 594, 801
BseXI GCAGC 1 cut(s) 95
BseYI CCCAGC 1 cut(s) 331
Bsh1236I CGCG 2 cut(s) 152, 675
BshFI GGCC 4 cut(s) 89, 318, 799, 963
BshNI GGYRCC 2 cut(s) 772, 838
BsiSI CCGG 3 cut(s) 44, 319, 815
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 5 cut(s) 478, 479, 511, 656, 668
BsmAI GTCTC 1 cut(s) 760
BsmFI GGGAC 1 cut(s) 455
BsnI GGCC 4 cut(s) 89, 318, 799, 963
Bsp120I GGGCCC 1 cut(s) 797
Bsp1286I GDGCHC 2 cut(s) 594, 801
Bsp13I TCCGGA 1 cut(s) 43
Bsp143I GATC 5 cut(s) 40, 59, 139, 175, 694
BspACI CCGC 9 cut(s) 73, 86, 150, 152, 188, 479, 505, 675, 842
BspANI GGCC 4 cut(s) 89, 318, 799, 963
BspCNI CTCAG 1 cut(s) 184
BspEI TCCGGA 1 cut(s) 43
BspFNI CGCG 2 cut(s) 152, 675
BspLI GGNNCC 6 cut(s) 141, 400, 660, 774, 799, 840
BspPI GGATC 3 cut(s) 35, 134, 147
BspT107I GGYRCC 2 cut(s) 772, 838
BsrFI RCCGGY 1 cut(s) 814
BssAI RCCGGY 1 cut(s) 814
BssECI CCNNGG 6 cut(s) 131, 150, 402, 685, 777, 800
BssMI GATC 5 cut(s) 40, 59, 139, 175, 694
BssT1I CCWWGG 1 cut(s) 777
Bst2UI CCWGG 2 cut(s) 802, 965
Bst4CI ACNGT 1 cut(s) 863
Bst6I CTCTTC 5 cut(s) 105, 231, 237, 255, 282
BstBAI YACGTR 1 cut(s) 545
BstC8I GCNNGC 3 cut(s) 91, 329, 897
BstDEI CTNAG 1 cut(s) 192
BstDSI CCRYGG 2 cut(s) 150, 402
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 3 cut(s) 28, 178, 412
BstFNI CGCG 2 cut(s) 152, 675
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 5 cut(s) 43, 62, 142, 178, 697
BstMAI GTCTC 1 cut(s) 760
BstMBI GATC 5 cut(s) 40, 59, 139, 175, 694
BstMWI GCNNNNNNNGC 2 cut(s) 324, 625
BstNI CCWGG 2 cut(s) 802, 965
BstSCI CCNGG 3 cut(s) 318, 800, 963
BstSLI GKGCMC 2 cut(s) 594, 801
BstUI CGCG 2 cut(s) 152, 675
BstV1I GCAGC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 4 cut(s) 89, 318, 799, 963
BtgI CCRYGG 2 cut(s) 150, 402
BtgZI GCGATG 1 cut(s) 83
BtsCI GGATG 3 cut(s) 28, 178, 412
BtsIMutI CAGTG 1 cut(s) 868
Cac8I GCNNGC 3 cut(s) 91, 329, 897
CaiI CAGNNNCTG 1 cut(s) 959
CfoI GCGC 1 cut(s) 630
Cfr10I RCCGGY 1 cut(s) 814
Cfr13I GGNCC 4 cut(s) 399, 659, 797, 798
Cfr42I CCGCGG 1 cut(s) 153
Csp6I GTAC 3 cut(s) 773, 868, 911
CviAII CATG 1 cut(s) 497
CviQI GTAC 3 cut(s) 773, 868, 911
DdeI CTNAG 1 cut(s) 192
DpnI GATC 5 cut(s) 42, 61, 141, 177, 696
DpnII GATC 5 cut(s) 40, 59, 139, 175, 694
DraIII CACNNNGTG 1 cut(s) 548
EaeI YGGCCR 2 cut(s) 87, 961
Eam1104I CTCTTC 5 cut(s) 105, 231, 237, 255, 282
EarI CTCTTC 5 cut(s) 105, 231, 237, 255, 282
Eco130I CCWWGG 1 cut(s) 777
Eco24I GRGCYC 1 cut(s) 801
Eco32I GATATC 1 cut(s) 305
Eco47I GGWCC 2 cut(s) 399, 659
Eco57I CTGAAG 2 cut(s) 450, 493
Eco72I CACGTG 1 cut(s) 545
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoO109I RGGNCCY 2 cut(s) 659, 797
EcoRII CCWGG 2 cut(s) 800, 963
EcoRV GATATC 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 777
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 801
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 1 cut(s) 500
FaqI GGGAC 1 cut(s) 455
FatI CATG 1 cut(s) 496
FauI CCCGC 1 cut(s) 472
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 4 cut(s) 84, 87, 150, 189
FokI GGATG 3 cut(s) 35, 185, 419
FriOI GRGCYC 1 cut(s) 801
Fsp4HI GCNGC 4 cut(s) 84, 87, 150, 189
FspBI CTAG 2 cut(s) 215, 975
GlaI GCGC 1 cut(s) 629
GluI GCNGC 4 cut(s) 84, 87, 150, 189
GsaI CCCAGC 1 cut(s) 335
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 4 cut(s) 89, 318, 799, 963
HapII CCGG 3 cut(s) 44, 319, 815
HhaI GCGC 1 cut(s) 630
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HindIII AAGCTT 1 cut(s) 999
HinfI GANTC 4 cut(s) 167, 365, 566, 956
HpaII CCGG 3 cut(s) 44, 319, 815
HphI GGTGA 3 cut(s) 119, 263, 386
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 5 cut(s) 144, 163, 172, 384, 765
Hpy188III TCNNGA 3 cut(s) 44, 308, 888
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 2 cut(s) 98, 124
HpyCH4III ACNGT 1 cut(s) 863
HpyCH4IV ACGT 3 cut(s) 544, 873, 909
HpyCH4V TGCA 4 cut(s) 467, 496, 619, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 324, 625
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 3 cut(s) 544, 873, 909
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 1 cut(s) 628
Kpn2I TCCGGA 1 cut(s) 43
KpnI GGTACC 1 cut(s) 776
Ksp22I TGATCA 1 cut(s) 175
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 5 cut(s) 40, 59, 139, 175, 694
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 1 cut(s) 60
MaeI CTAG 2 cut(s) 215, 975
MaeII ACGT 3 cut(s) 544, 873, 909
MaeIII GTNAC 1 cut(s) 49
MalI GATC 5 cut(s) 42, 61, 141, 177, 696
MboI GATC 5 cut(s) 40, 59, 139, 175, 694
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 2 cut(s) 594, 801
MlsI TGGCCA 1 cut(s) 963
MluCI AATT 2 cut(s) 919, 984
MluNI TGGCCA 1 cut(s) 963
MlyI GAGTC 1 cut(s) 965
MmeI TCCRAC 2 cut(s) 276, 362
Mox20I TGGCCA 1 cut(s) 963
Mph1103I ATGCAT 1 cut(s) 498
MroI TCCGGA 1 cut(s) 43
MscI TGGCCA 1 cut(s) 963
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 162
Msp20I TGGCCA 1 cut(s) 963
MspA1I CMGCKG 3 cut(s) 86, 152, 479
MspI CCGG 3 cut(s) 44, 319, 815
MspR9I CCNGG 3 cut(s) 320, 802, 965
MvaI CCWGG 2 cut(s) 802, 965
MvnI CGCG 2 cut(s) 152, 675
MwoI GCNNNNNNNGC 2 cut(s) 324, 625
NciI CCSGG 1 cut(s) 320
NdeII GATC 5 cut(s) 40, 59, 139, 175, 694
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 6 cut(s) 141, 400, 660, 774, 799, 840
NmeAIII GCCGAG 2 cut(s) 156, 925
NsiI ATGCAT 1 cut(s) 498
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 535
PfeI GAWTC 3 cut(s) 167, 365, 566
PkrI GCNGC 4 cut(s) 85, 88, 151, 190
PleI GAGTC 1 cut(s) 964
PmaCI CACGTG 1 cut(s) 545
PmlI CACGTG 1 cut(s) 545
PpsI GAGTC 1 cut(s) 964
Ppu21I YACGTR 1 cut(s) 545
PpuMI RGGWCCY 1 cut(s) 659
Psp5II RGGWCCY 1 cut(s) 659
Psp6I CCWGG 2 cut(s) 800, 963
PspCI CACGTG 1 cut(s) 545
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 2 cut(s) 800, 963
PspN4I GGNNCC 6 cut(s) 141, 400, 660, 774, 799, 840
PspOMI GGGCCC 1 cut(s) 797
PspPI GGNCC 4 cut(s) 399, 659, 797, 798
PspPPI RGGWCCY 1 cut(s) 659
PstNI CAGNNNCTG 1 cut(s) 959
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 3 cut(s) 774, 869, 912
RsaNI GTAC 3 cut(s) 773, 868, 911
RseI CAYNNNNRTG 1 cut(s) 162
SacII CCGCGG 1 cut(s) 153
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 4 cut(s) 84, 87, 150, 189
Sau3AI GATC 5 cut(s) 40, 59, 139, 175, 694
Sau96I GGNCC 4 cut(s) 399, 659, 797, 798
SchI GAGTC 1 cut(s) 965
ScrFI CCNGG 3 cut(s) 320, 802, 965
SduI GDGCHC 2 cut(s) 594, 801
SfaNI GCATC 1 cut(s) 60
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 2 cut(s) 399, 659
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 996
SmoI CTYRAG 1 cut(s) 996
Sse9I AATT 2 cut(s) 919, 984
SsiI CCGC 9 cut(s) 73, 86, 150, 152, 188, 479, 505, 675, 842
SspMI CTAG 2 cut(s) 215, 975
StyD4I CCNGG 3 cut(s) 318, 800, 963
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 1 cut(s) 863
TaiI ACGT 3 cut(s) 547, 876, 912
TaqI TCGA 1 cut(s) 368
TasI AATT 2 cut(s) 919, 984
TatI WGTACW 1 cut(s) 867
TauI GCSGC 3 cut(s) 89, 152, 191
TfiI GAWTC 3 cut(s) 167, 365, 566
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 1 cut(s) 868
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 4 cut(s) 180, 471, 591, 825
TspGWI ACGGA 2 cut(s) 137, 391
TspRI CASTG 1 cut(s) 868
VpaK11BI GGWCC 2 cut(s) 399, 659
XagI CCTNNNNNAGG 1 cut(s) 666
XapI RAATTY 1 cut(s) 984
XspI CTAG 2 cut(s) 215, 975
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.